AlfalfaGEDB | Msa0809990

M. sativa cultivar zhongmu-4 / Msa0809990

KEGG: K11254
GO: GO:0000228 GO:0000785 GO:0000786 GO:0000788 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0031497 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0034622 GO:0034728 GO:0042393 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044815 GO:0051276 GO:0065003 GO:0065004 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0097159 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0809990	sp\|P0CG89\|H4_SOYBN	100.000	103	0	0	1	103	1	103	3.06e-67	199

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0809990	A0A453MZH1	100.000	103	0	0	1	103	31	133	3.54e-66	204

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0809990	Msa1373100	0.812252	4.655853e-51	-8.615850e-47
Msa0809990	Msa1422950	0.812006	5.268378e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
Msa0809990	MtrunA17_Chr4g0072411	100.000	103	1	103	59	161	5.39e-69	203
Msa0809990	MtrunA17_Chr5g0425951	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr4g0030431	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr3g0100121	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr3g0113681	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr2g0327641	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr3g0103351	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr8g0352791	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr3g0103821	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr3g0104031	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr7g0225731	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr3g0103801	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr3g0103881	100.000	103	1	103	1	103	2.26e-68	199
Msa0809990	MtrunA17_Chr7g0262791	100.000	103	1	103	1	103	2.26e-68	199

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0809990	AT3G53730.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT1G07820.2	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT1G07820.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT5G59690.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT3G46320.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT2G28740.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT1G07660.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT3G45930.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT5G59970.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT5G59970.2	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0809990	AT1G07660.2	83.495	103	1	1	103	1	86	4.52e-51	155

Find 28 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGAACCCTTTATGGATT+TGG	0.242143	5_3:-37858193	Msa0809990:CDS
AGGCAAGGAAGAACCCTTTA+TGG	0.262279	5_3:-37858199	Msa0809990:CDS
GAAGAACCCTTTATGGATTT+GGG	0.291468	5_3:-37858192	Msa0809990:CDS
GGCAAAGGAGGAAAGGGTTT+AGG	0.384869	5_3:-37858463	Msa0809990:CDS
AAGACGACGAATCGCGGGTT+TGG	0.418134	5_3:+37858384	None:intergenic
GTTCTTCGAGATAACATTCA+AGG	0.432889	5_3:-37858412	Msa0809990:CDS
TCGCAAAACGGTGACTGCTA+TGG	0.458269	5_3:-37858242	Msa0809990:CDS
ATCTTGCAATCAATCATGTC+AGG	0.507815	5_3:-37858490	None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG	0.517349	5_3:-37858457	Msa0809990:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	5_3:-37858470	Msa0809990:CDS
CCGCGATTCGTCGTCTTGCA+AGG	0.525617	5_3:-37858378	Msa0809990:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	5_3:-37858469	Msa0809990:CDS
CTTGCAAGACGACGAATCGC+GGG	0.554223	5_3:+37858379	None:intergenic
TGAACATGCTCGTCGCAAAA+CGG	0.556730	5_3:-37858254	Msa0809990:CDS
CAATCAATCATGTCAGGAAG+AGG	0.559993	5_3:-37858484	None:intergenic
CCTTGCAAGACGACGAATCG+CGG	0.571292	5_3:+37858378	None:intergenic
ATCATGTCAGGAAGAGGCAA+AGG	0.584537	5_3:-37858478	Msa0809990:CDS
AAGAACCCTTTATGGATTTG+GGG	0.597685	5_3:-37858191	Msa0809990:CDS
GTTTATGCACTCAAGAGGCA+AGG	0.602465	5_3:-37858214	Msa0809990:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.604597	5_3:-37858475	Msa0809990:CDS
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	5_3:-37858370	Msa0809990:CDS
AGGAGGAAAGGGTTTAGGAA+AGG	0.616515	5_3:-37858458	Msa0809990:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	5_3:-37858373	Msa0809990:CDS
ATGTTGTTTATGCACTCAAG+AGG	0.622074	5_3:-37858219	Msa0809990:CDS
AGAACCCTTTATGGATTTGG+GGG	0.634757	5_3:-37858190	Msa0809990:CDS
GGTGGTGTAAAGCGTATCAG+TGG	0.649124	5_3:-37858352	Msa0809990:CDS
CTCATCTATGAAGAAACTCG+TGG	0.682677	5_3:-37858328	Msa0809990:CDS
GGAAAGGGTTTAGGAAAGGG+CGG	0.700891	5_3:-37858454	Msa0809990:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTCTTCGAGATAACATTCA+AGG	-	chr5_3:37858249-37858268	Msa0809990:CDS	35.0%
!	ATGTTGTTTATGCACTCAAG+AGG	-	chr5_3:37858442-37858461	Msa0809990:CDS	35.0%
	GAAGAACCCTTTATGGATTT+GGG	-	chr5_3:37858469-37858488	Msa0809990:CDS	35.0%
	AAGAACCCTTTATGGATTTG+GGG	-	chr5_3:37858470-37858489	Msa0809990:CDS	35.0%
	CTCATCTATGAAGAAACTCG+TGG	-	chr5_3:37858333-37858352	Msa0809990:CDS	40.0%
	GGAAGAACCCTTTATGGATT+TGG	-	chr5_3:37858468-37858487	Msa0809990:CDS	40.0%
	AGAACCCTTTATGGATTTGG+GGG	-	chr5_3:37858471-37858490	Msa0809990:CDS	40.0%
	CTAACCCCCAAATCCATAAA+GGG	+	chr5_3:37858478-37858497	None:intergenic	40.0%
	AGGAGGAAAGGGTTTAGGAA+AGG	-	chr5_3:37858203-37858222	Msa0809990:CDS	45.0%
	GGAGGAAAGGGTTTAGGAAA+GGG	-	chr5_3:37858204-37858223	Msa0809990:CDS	45.0%
	TGAACATGCTCGTCGCAAAA+CGG	-	chr5_3:37858407-37858426	Msa0809990:CDS	45.0%
	GTTTATGCACTCAAGAGGCA+AGG	-	chr5_3:37858447-37858466	Msa0809990:CDS	45.0%
	AGGCAAGGAAGAACCCTTTA+TGG	-	chr5_3:37858462-37858481	Msa0809990:CDS	45.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr5_3:37858186-37858205	Msa0809990:CDS	50.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr5_3:37858191-37858210	Msa0809990:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr5_3:37858192-37858211	Msa0809990:CDS	50.0%
	GGCAAAGGAGGAAAGGGTTT+AGG	-	chr5_3:37858198-37858217	Msa0809990:CDS	50.0%
	GGAAAGGGTTTAGGAAAGGG+CGG	-	chr5_3:37858207-37858226	Msa0809990:CDS	50.0%
	GGTGGTGTAAAGCGTATCAG+TGG	-	chr5_3:37858309-37858328	Msa0809990:CDS	50.0%
	TCGCAAAACGGTGACTGCTA+TGG	-	chr5_3:37858419-37858438	Msa0809990:CDS	50.0%
	AAGACGACGAATCGCGGGTT+TGG	+	chr5_3:37858280-37858299	None:intergenic	55.0%
	CTTGCAAGACGACGAATCGC+GGG	+	chr5_3:37858285-37858304	None:intergenic	55.0%
	CCTTGCAAGACGACGAATCG+CGG	+	chr5_3:37858286-37858305	None:intergenic	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	-	chr5_3:37858288-37858307	Msa0809990:CDS	55.0%
	CCGCGATTCGTCGTCTTGCA+AGG	-	chr5_3:37858283-37858302	Msa0809990:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	-	chr5_3:37858291-37858310	Msa0809990:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	37858186	37858497	37858186	ID=Msa0809990;Name=Msa0809990
chr5_3	mRNA	37858186	37858497	37858186	ID=Msa0809990-mRNA-1;Parent=Msa0809990;Name=Msa0809990-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|103
chr5_3	exon	37858186	37858497	37858186	ID=Msa0809990-mRNA-1:exon:11599;Parent=Msa0809990-mRNA-1
chr5_3	CDS	37858186	37858497	37858186	ID=Msa0809990-mRNA-1:cds;Parent=Msa0809990-mRNA-1

Gene Sequence	>Msa0809990 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGCGGTGCAAAGCGTCATCGCAAAGTTCTTCGAGATAACATTCAAGGTATCACCAAACCCGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTAAAGCGTATCAGTGGTCTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATATTTCTTGAGAATGTTATTCGTGATGCTGTCACTTACACTGAACATGCTCGTCGCAAAACGGTGACTGCTATGGATGTTGTTTATGCACTCAAGAGGCAAGGAAGAACCCTTTATGGATTTGGGGGTTAG
Protein sequence	>Msa0809990 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*