AlfalfaGEDB | Msa0816550

M. sativa cultivar zhongmu-4 / Msa0816550

KEGG: K09422
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816550	XP_003616388.1	95.872	218	4	1	1	218	1	213	1.30e-149	424

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816550	sp\|F1B281\|EBIII_PETHY	55.440	193	80	2	2	194	3	189	5.02e-73	225

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816550	G7KBP3	95.872	218	4	1	1	218	1	213	6.23e-150	424

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TFs/TRs:

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Gene ID	Type	Classification
Msa0816550	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0816520	Msa0816550	0.896485	3.524614e-76	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816550	MtrunA17_Chr5g0435101	95.872	218	4	1	1	218	1	213	1.20e-153	424
Msa0816550	MtrunA17_Chr4g0044421	72.917	144	38	1	1	143	1	144	1.44e-72	218
Msa0816550	MtrunA17_Chr4g0037141	72.800	125	34	0	2	126	3	127	1.53e-69	214
Msa0816550	MtrunA17_Chr3g0131121	71.200	125	36	0	2	126	3	127	1.21e-68	212
Msa0816550	MtrunA17_Chr8g0385321	68.000	125	40	0	2	126	3	127	1.24e-64	202
Msa0816550	MtrunA17_Chr2g0281071	68.000	125	39	1	2	125	3	127	2.93e-62	197
Msa0816550	MtrunA17_Chr4g0048511	67.200	125	40	1	2	125	3	127	1.67e-61	196
Msa0816550	MtrunA17_Chr1g0193371	65.600	125	43	0	2	126	3	127	4.21e-61	195
Msa0816550	MtrunA17_Chr5g0410961	66.400	125	42	0	2	126	3	127	5.90e-61	194
Msa0816550	MtrunA17_Chr3g0115821	62.400	125	47	0	2	126	3	127	7.93e-61	194

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816550	AT4G38620.1	51.905	210	79	3	2	194	3	207	9.67e-72	220
Msa0816550	AT1G22640.1	73.016	126	34	0	2	127	3	128	3.10e-71	218
Msa0816550	AT2G16720.1	48.908	229	96	6	2	218	3	222	2.68e-70	216
Msa0816550	AT4G09460.1	72.000	125	35	0	2	126	3	127	2.33e-69	212
Msa0816550	AT4G34990.1	50.490	204	88	3	2	199	3	199	1.26e-67	209
Msa0816550	AT1G35515.1	50.244	205	95	3	2	200	3	206	3.14e-66	203
Msa0816550	AT4G28110.1	66.129	124	42	0	2	125	3	126	6.33e-64	200
Msa0816550	AT4G21440.1	60.274	146	51	1	2	147	3	141	7.98e-64	202
Msa0816550	AT5G16770.4	66.400	125	42	0	2	126	3	127	5.72e-63	199
Msa0816550	AT5G16770.3	66.400	125	42	0	2	126	3	127	5.72e-63	199

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Find 51 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATCAACAAAGGTGCT+TGG	0.548391	5_3:-51035680	Msa0816550:CDS
AAAGAAACATCATTATTAAC+GGG	0.455198	5_3:+51034243	None:intergenic
AACTAATTACCAGCAGCCTT+GGG	0.489756	5_3:+51035588	None:intergenic
AAGATTCCACGTTTGATATC+TGG	0.258514	5_3:+51035318	None:intergenic
AAGTTCATGGTGAAGGATGT+TGG	0.511367	5_3:-51035620	Msa0816550:CDS
AATAACCACAAACTACACAA+AGG	0.683810	5_3:-51034363	Msa0816550:CDS
ACCTATTGCCAAGGAGGGCA+TGG	0.478073	5_3:+51035262	None:intergenic
ACCTCATTATCTGTACGTCC+AGG	0.536658	5_3:+51034444	None:intergenic
ACTGTAGACACATGAGGACT+AGG	0.574965	5_3:+51034336	None:intergenic
AGGATTGTTATACCAAATTG+TGG	0.488222	5_3:-51034138	Msa0816550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GACAAAGAAAACATCAACAA+AGG	-	chr5_3:51034113-51034132	Msa0816550:CDS	30.0%
TATATACAAGTTCATGGTGA+AGG	-	chr5_3:51034173-51034192	Msa0816550:CDS	30.0%
AAGTTCAACGACTCTTTAAT+AGG	-	chr5_3:51034342-51034361	Msa0816550:CDS	30.0%
AATACTTTAGGTCAATCGTT+TGG	-	chr5_3:51034757-51034776	Msa0816550:intron	30.0%
AACATATTTCCTTATTAGGG+AGG	+	chr5_3:51035192-51035211	None:intergenic	30.0%
AATAACCACAAACTACACAA+AGG	-	chr5_3:51035437-51035456	Msa0816550:intron	30.0%
ATAACAATCCTATTTGGAGA+AGG	+	chr5_3:51035653-51035672	None:intergenic	30.0%
AGGATTGTTATACCAAATTG+TGG	-	chr5_3:51035662-51035681	Msa0816550:CDS	30.0%
GGATTGTTATACCAAATTGT+GGG	-	chr5_3:51035663-51035682	Msa0816550:CDS	30.0%
GACAAAGAAAACATCAACAA+AGG	-	chr5_3:51034113-51034132	Msa0816550:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	51034095	51035727	51034095	ID=Msa0816550;Name=Msa0816550
chr5_3	mRNA	51034095	51035727	51034095	ID=Msa0816550-mRNA-1;Parent=Msa0816550;Name=Msa0816550-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|3\|0\|218
chr5_3	exon	51035598	51035727	51035598	ID=Msa0816550-mRNA-1:exon:15314;Parent=Msa0816550-mRNA-1
chr5_3	exon	51035264	51035393	51035264	ID=Msa0816550-mRNA-1:exon:15313;Parent=Msa0816550-mRNA-1
chr5_3	exon	51034095	51034491	51034095	ID=Msa0816550-mRNA-1:exon:15312;Parent=Msa0816550-mRNA-1
chr5_3	CDS	51035598	51035727	51035598	ID=Msa0816550-mRNA-1:cds;Parent=Msa0816550-mRNA-1
chr5_3	CDS	51035264	51035393	51035264	ID=Msa0816550-mRNA-1:cds;Parent=Msa0816550-mRNA-1
chr5_3	CDS	51034095	51034491	51034095	ID=Msa0816550-mRNA-1:cds;Parent=Msa0816550-mRNA-1

Gene Sequence

>Msa0816550

ATGAGAAAGCCTTGTTGTGACAAAGAAAACATCAACAAAGGTGCTTGGTCTAAGCAAGAAGATCAAAAGCTCATTGATTATATACAAGTTCATGGTGAAGGATGTTGGGGCTCCATTCCCAAGGCTGCTGGGTTGCATCGTTGTGGCAAAAGTTGTAGGCTAAGATGGTTAAACTACTTACGACCAGATATCAAACGTGGAATCTTTGCACAAGATGAAGAAGACCTTATCATCAAACTCCATGCCCTCCTTGGCAATAGGTGGGCACTCATAGCTGGAAGGTTGCCTGGACGTACAGATAATGAGGTGAAAAACTATTGGAATTCTCATATTCGTAGAAAGCTAATAAAAATGGGAATTGATCCAAATAACCACAAACTACACAAAGGCTTTCCTAGTCCTCATGTGTCTACAGTGGGCACATCATCATGTGTTGAGTCCATGAATAAAGAGAATAATAATCTCTCCATCAAGTCGTGTGATGCACCCGTTAATAATGATGTTTCTTTCGTAAAGAAAGATACTACATCAATAATTAATTCTTCATCATCTTTGAATCTTGACTTAACCATTGCTTTACCTTCTCCAAATAGGATTGTTATACCAAATTGTGGGTCTCCTAAGACTAGGGATATGGATATTGATCTTAATTGTTAA

Protein sequence

>Msa0816550

MRKPCCDKENINKGAWSKQEDQKLIDYIQVHGEGCWGSIPKAAGLHRCGKSCRLRWLNYLRPDIKRGIFAQDEEDLIIKLHALLGNRWALIAGRLPGRTDNEVKNYWNSHIRRKLIKMGIDPNNHKLHKGFPSPHVSTVGTSSCVESMNKENNNLSIKSCDAPVNNDVSFVKKDTTSIINSSSSLNLDLTIALPSPNRIVIPNCGSPKTRDMDIDLNC*