AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0817470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817470 MtrunA17_Chr5g0436201 97.183 213 4 2 1 213 1 211 4.29e-151 417
Msa0817470 MtrunA17_Chr3g0112791 56.422 218 68 5 1 213 22 217 1.29e-69 211
Msa0817470 MtrunA17_Chr7g0240961 53.431 204 85 6 13 213 38 234 2.15e-62 194
Msa0817470 MtrunA17_Chr2g0284111 38.686 137 65 3 87 211 153 282 2.77e-29 110
Msa0817470 MtrunA17_Chr4g0045171 37.222 180 81 5 44 211 139 298 3.15e-28 107
Msa0817470 MtrunA17_Chr8g0379191 36.667 180 82 5 44 211 150 309 7.82e-27 104
Msa0817470 MtrunA17_Chr3g0112811 60.241 83 23 2 59 136 46 123 3.28e-21 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817470 AT5G05830.1 63.580 162 54 3 54 213 45 203 6.31e-68 207
Msa0817470 AT5G01070.1 53.077 130 58 1 87 213 75 204 4.57e-42 141
Msa0817470 AT2G37950.1 50.769 130 56 3 87 213 81 205 2.66e-39 134
Msa0817470 AT5G59000.1 38.967 213 68 7 52 211 26 229 3.41e-33 119
Msa0817470 AT2G34200.2 44.944 89 38 2 87 168 99 183 3.87e-17 77.0
Msa0817470 AT2G34200.1 44.944 89 38 2 87 168 99 183 3.87e-17 77.0

Find 74 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACACAATTCATTAACCTTT+TGG 0.244579 5_3:-52777711 Msa0817470:five_prime_UTR
GAGGCTTGGTTCAAGATTAA+AGG 0.278430 5_3:-52777192 Msa0817470:CDS
TGTCTCTCCATAGTTTGATT+TGG 0.297388 5_3:-52775307 Msa0817470:three_prime_UTR
TAGGATTTGTCATCTTAGTA+TGG 0.302408 5_3:-52777301 Msa0817470:CDS
TTCTGATGTTAAGGTGCATT+TGG 0.316270 5_3:-52777343 Msa0817470:CDS
GAATTTCCTTCTAGCATGTA+TGG 0.346946 5_3:-52775456 Msa0817470:CDS
TTACATGAAGATTCTCATCT+TGG 0.360541 5_3:-52777603 Msa0817470:five_prime_UTR
TACATCTCCAAATCAAACTA+TGG 0.373623 5_3:+52775300 None:intergenic
TTTAGTTTAATGTTGTGTTT+TGG 0.374492 5_3:-52775059 Msa0817470:three_prime_UTR
GATATGACAAATCATGAATC+TGG 0.374563 5_3:-52777279 Msa0817470:CDS
ATAAGGTGGAGAGGGATTGT+AGG 0.391162 5_3:-52777320 Msa0817470:CDS
CCCTCCATTGTACCACCAAC+TGG 0.414352 5_3:-52775530 Msa0817470:CDS
TTTGATCCTCCTCATTTAAC+CGG 0.432004 5_3:+52777478 None:intergenic
ATAAGCATTGTGCTGAGGCT+TGG 0.438291 5_3:-52777206 Msa0817470:CDS
TCTTGGTTGTTTCAAAATGC+AGG 0.445406 5_3:-52777586 Msa0817470:exon
AAACTATCTTCATCAAACTT+TGG 0.447618 5_3:-52777659 Msa0817470:five_prime_UTR
GATCAAAGGTGTGAGATTGT+AGG 0.451686 5_3:-52777462 Msa0817470:CDS
TGTAGAGGTTTCTGATGTTA+AGG 0.455190 5_3:-52777352 Msa0817470:CDS
ATGTTCAGTGGAGTTGGATC+TGG 0.459424 5_3:-52777382 Msa0817470:CDS
ACAATACAATTTCTACATGA+TGG 0.464179 5_3:+52775125 None:intergenic
TGTATATATAATTAATATTG+AGG 0.468168 5_3:-52775036 Msa0817470:three_prime_UTR
CTATCTTCATCAAACTTTGG+TGG 0.476920 5_3:-52777656 Msa0817470:five_prime_UTR
ACATCCTTTGATACTATCCT+AGG 0.479401 5_3:+52775206 None:intergenic
TTTCAAAATGCAGGTAAATG+AGG 0.481861 5_3:-52777577 Msa0817470:exon
TCATGAATCTGGAATTCCTA+TGG 0.482383 5_3:-52777268 Msa0817470:CDS
ATCAGAATGTTCAGTGGAGT+TGG 0.482682 5_3:-52777388 Msa0817470:CDS
TGAAGCAAGTAACATGATAG+AGG 0.503631 5_3:-52775363 Msa0817470:three_prime_UTR
CGAGTTTCTGATGGTGGTGA+AGG 0.510573 5_3:+52775506 None:intergenic
TCTGGAATTCCTATGGAGCT+TGG 0.510778 5_3:-52777261 Msa0817470:CDS
TGTTAAGGTGCATTTGGATA+AGG 0.511468 5_3:-52777337 Msa0817470:CDS
TAGAACATCGTTCATTGCTC+CGG 0.512528 5_3:+52777511 None:intergenic
GCATTTGGATAAGGTGGAGA+GGG 0.524811 5_3:-52777328 Msa0817470:CDS
TAAGGTGCATTTGGATAAGG+TGG 0.527254 5_3:-52777334 Msa0817470:CDS
TCAAACTATGGAGAGACATA+AGG 0.529923 5_3:+52775312 None:intergenic
GTTGAAATTGATAGACATGT+TGG 0.534239 5_3:-52775104 Msa0817470:three_prime_UTR
GGATCTGGATGGTGGTGTAG+AGG 0.539432 5_3:-52777367 Msa0817470:CDS
CTCCTCATTTAACCGGTCAC+CGG 0.540911 5_3:+52777485 None:intergenic
ATATTACTCTACACTGTGGT+AGG 0.547584 5_3:+52777741 None:intergenic
TCCAGTTGGTGGTACAATGG+AGG 0.550684 5_3:+52775529 None:intergenic
CCAGTTGGTGGTACAATGGA+GGG 0.553362 5_3:+52775530 None:intergenic
CATTTGAACTTCCATAGCAC+CGG 0.554330 5_3:+52775580 None:intergenic
GATGTTCTAACTCCGGTGAC+CGG 0.563674 5_3:-52777497 Msa0817470:CDS
CTCCGGTGACCGGTTAAATG+AGG 0.569406 5_3:-52777487 Msa0817470:CDS
ACACAATGTAGCCGGTGCTA+TGG 0.576578 5_3:-52775591 Msa0817470:CDS
TCAATTGCACACAATGTAGC+CGG 0.585989 5_3:-52775599 Msa0817470:CDS
GGTGGTGAAGGTCCAGTTGG+TGG 0.586158 5_3:+52775518 None:intergenic
TTGAATTCCTGTGTAACTTG+AGG 0.588291 5_3:-52775387 Msa0817470:exon
TCAACCTAGGATAGTATCAA+AGG 0.589792 5_3:-52775210 Msa0817470:three_prime_UTR
TGCTCATAAGCATTGTGCTG+AGG 0.590807 5_3:-52777211 Msa0817470:CDS
TGCATTTGGATAAGGTGGAG+AGG 0.590996 5_3:-52777329 Msa0817470:CDS
GGTTAAATGAGGAGGATCAA+AGG 0.593905 5_3:-52777476 Msa0817470:CDS
TAATTAATATTGAGGAGTGA+AGG 0.594633 5_3:-52775028 None:intergenic
GGACAAGTTTCATGTTGTTG+TGG 0.595194 5_3:-52777556 Msa0817470:CDS
CTTCACCACCATCAGAAACT+CGG 0.596501 5_3:-52775505 Msa0817470:CDS
AAATTCCGAGTTTCTGATGG+TGG 0.598667 5_3:+52775500 None:intergenic
GCTTCATCCTCAAGTTACAC+AGG 0.600922 5_3:+52775380 None:intergenic
AGGTCCAGTTGGTGGTACAA+TGG 0.605202 5_3:+52775526 None:intergenic
GATGGTGGTGAAGGTCCAGT+TGG 0.613111 5_3:+52775515 None:intergenic
TAGACAACAATGTATGTCAC+CGG 0.620225 5_3:-52777530 Msa0817470:CDS
AATGAACGATGTTCTAACTC+CGG 0.623265 5_3:-52777504 Msa0817470:CDS
AACAGAACTTGTGAAATCTG+TGG 0.623786 5_3:-52775623 Msa0817470:intron
CGGTGACCGGTTAAATGAGG+AGG 0.626754 5_3:-52777484 Msa0817470:CDS
GAAGCAAGTAACATGATAGA+GGG 0.636600 5_3:-52775362 Msa0817470:three_prime_UTR
ATCTGAATCAGAATGTTCAG+TGG 0.639058 5_3:-52777394 Msa0817470:CDS
GTGGAGTTGGATCTGGATGG+TGG 0.642032 5_3:-52777375 Msa0817470:CDS
TGATAGACATGTTGGACATG+TGG 0.646479 5_3:-52775096 Msa0817470:three_prime_UTR
TGATAGAGGGTTATGAACAC+AGG 0.646635 5_3:-52775349 Msa0817470:three_prime_UTR
AAGTTACACAGGAATTCAAG+AGG 0.657142 5_3:+52775391 None:intergenic
TCAGTGGAGTTGGATCTGGA+TGG 0.667485 5_3:-52777378 Msa0817470:CDS
AGTTACACAGGAATTCAAGA+GGG 0.673281 5_3:+52775392 None:intergenic
GGAGTGTCTGAGAAACAAAG+AGG 0.682144 5_3:-52777441 Msa0817470:CDS
TAGACATGTTGGACATGTGG+AGG 0.701825 5_3:-52775093 Msa0817470:three_prime_UTR
CAGTTGGTGGTACAATGGAG+GGG 0.714794 5_3:+52775531 None:intergenic
AAAGATATTACTCTACACTG+TGG 0.726392 5_3:+52777737 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATATATAATTAATATTG+AGG - chr5_3:52777817-52777836 Msa0817470:five_prime_UTR 10.0%
!!! TGATCATAAATTTTTATGTT+TGG + chr5_3:52776033-52776052 None:intergenic 15.0%
!! AAATTGAACATATAATTGTA+TGG - chr5_3:52776726-52776745 Msa0817470:intron 15.0%
!! AATATATTGAAATCAAAACT+TGG + chr5_3:52776816-52776835 None:intergenic 15.0%
!!! ATGTTTTTGCAAAAATGATT+TGG - chr5_3:52775615-52775634 Msa0817470:CDS 20.0%
!! TAATCTATGTTTGAATAGTT+TGG - chr5_3:52775924-52775943 Msa0817470:intron 20.0%
!!! TTTTAGGTAAAATTGATTTG+TGG - chr5_3:52775999-52776018 Msa0817470:intron 20.0%
!!! ATTCTTCTTTTGTAATCAAA+AGG - chr5_3:52776421-52776440 Msa0817470:intron 20.0%
!! AATGGTTGAAAGAAAAATAT+TGG - chr5_3:52776501-52776520 Msa0817470:intron 20.0%
!! AATGTAAAGTGAAATTTGAA+AGG - chr5_3:52777044-52777063 Msa0817470:intron 20.0%
!! CAAGTTAAATTGTTTAATGT+TGG - chr5_3:52777112-52777131 Msa0817470:intron 20.0%
!!! TTTAGTTTAATGTTGTGTTT+TGG - chr5_3:52777794-52777813 Msa0817470:five_prime_UTR 20.0%
! AAACTATCTTCATCAAACTT+TGG - chr5_3:52775194-52775213 Msa0817470:three_prime_UTR 25.0%
! ACATATCAACTCAAATATAG+TGG + chr5_3:52775762-52775781 None:intergenic 25.0%
! ATCTACAATTTCATTCAATG+TGG + chr5_3:52775812-52775831 None:intergenic 25.0%
!! ACAATGTATAAGAGTATGTT+TGG - chr5_3:52775837-52775856 Msa0817470:intron 25.0%
! TATGACAATAAACATAAGCT+AGG - chr5_3:52775967-52775986 Msa0817470:intron 25.0%
!!! AAATTTTTATGTTTGGCTTC+CGG + chr5_3:52776026-52776045 None:intergenic 25.0%
! AAACAAAATCTTAAGACATC+CGG + chr5_3:52776137-52776156 None:intergenic 25.0%
!!! TAAGATTTTGTTTGAAGATG+CGG - chr5_3:52776144-52776163 Msa0817470:intron 25.0%
!! CTCTTATGTTTTTCTTTTTG+TGG - chr5_3:52776237-52776256 Msa0817470:intron 25.0%
! AATAGCTTAAGATAAGGTAA+AGG + chr5_3:52776287-52776306 None:intergenic 25.0%
! AAGTCAAATAGCTTAAGATA+AGG + chr5_3:52776293-52776312 None:intergenic 25.0%
! TGAGCTAGATTATAATAGAA+AGG - chr5_3:52776467-52776486 Msa0817470:intron 25.0%
! GAGCTAGATTATAATAGAAA+GGG - chr5_3:52776468-52776487 Msa0817470:intron 25.0%
! GATTATAATAGAAAGGGTTA+AGG - chr5_3:52776474-52776493 Msa0817470:intron 25.0%
!!! CTGCTTTTATCTTCAATTAT+GGG - chr5_3:52776620-52776639 Msa0817470:intron 25.0%
!!! ATTGAGCTGATTTTTCAATT+TGG + chr5_3:52776662-52776681 None:intergenic 25.0%
!!! GGGATTCTTTTTTCTTTTAT+TGG + chr5_3:52776700-52776719 None:intergenic 25.0%
! AAAAGAAAAAAGAATCCCTT+TGG - chr5_3:52776702-52776721 Msa0817470:intron 25.0%
! AAAGAAAAAAGAATCCCTTT+GGG - chr5_3:52776703-52776722 Msa0817470:intron 25.0%
! TATATGTTCAATTTCCCAAA+GGG + chr5_3:52776720-52776739 None:intergenic 25.0%
! TTATATGTTCAATTTCCCAA+AGG + chr5_3:52776721-52776740 None:intergenic 25.0%
! ACTTTGTACAATTTCACTAT+AGG - chr5_3:52776907-52776926 Msa0817470:intron 25.0%
! ATATCTAATGATCAAACTAC+AGG - chr5_3:52777002-52777021 Msa0817470:intron 25.0%
! ACTACAGGAAGATTAATATA+CGG - chr5_3:52777017-52777036 Msa0817470:intron 25.0%
! AGATAGAACTGATTATTGAT+AGG - chr5_3:52777084-52777103 Msa0817470:intron 25.0%
! ACAATACAATTTCTACATGA+TGG + chr5_3:52777731-52777750 None:intergenic 25.0%
AAAGATATTACTCTACACTG+TGG + chr5_3:52775119-52775138 None:intergenic 30.0%
! CACACAATTCATTAACCTTT+TGG - chr5_3:52775142-52775161 Msa0817470:three_prime_UTR 30.0%
CTCAACAAAAAAGAACCAAA+AGG + chr5_3:52775160-52775179 None:intergenic 30.0%
TTACATGAAGATTCTCATCT+TGG - chr5_3:52775250-52775269 Msa0817470:three_prime_UTR 30.0%
TTTCAAAATGCAGGTAAATG+AGG - chr5_3:52775276-52775295 Msa0817470:three_prime_UTR 30.0%
TAGGATTTGTCATCTTAGTA+TGG - chr5_3:52775552-52775571 Msa0817470:CDS 30.0%
GATATGACAAATCATGAATC+TGG - chr5_3:52775574-52775593 Msa0817470:CDS 30.0%
TTCACAACTTCATTCCAAAA+GGG + chr5_3:52775710-52775729 None:intergenic 30.0%
ATTCACAACTTCATTCCAAA+AGG + chr5_3:52775711-52775730 None:intergenic 30.0%
!!! AGCTAGGTATATTAGCTTTT+AGG - chr5_3:52775983-52776002 Msa0817470:intron 30.0%
ACAAACATGGAACTAAACAT+AGG - chr5_3:52776091-52776110 Msa0817470:intron 30.0%
AGAGAATCCTAAAACACTAT+AGG + chr5_3:52776221-52776240 None:intergenic 30.0%
! TTCTTTTTGTGGAAAAGTTG+TGG - chr5_3:52776248-52776267 Msa0817470:intron 30.0%
ACTATGTAATGTCTTGATAG+TGG - chr5_3:52776386-52776405 Msa0817470:intron 30.0%
AGAAAGGGTTAAGGTAATAA+TGG - chr5_3:52776483-52776502 Msa0817470:intron 30.0%
CCATAATTGAAGATAAAAGC+AGG + chr5_3:52776622-52776641 None:intergenic 30.0%
!! CCTGCTTTTATCTTCAATTA+TGG - chr5_3:52776619-52776638 Msa0817470:intron 30.0%
! TTTTTGCCTTTGTCATATCA+TGG - chr5_3:52777420-52777439 Msa0817470:CDS 30.0%
AAAAAGCCATGATATGACAA+AGG + chr5_3:52777429-52777448 None:intergenic 30.0%
TACATCTCCAAATCAAACTA+TGG + chr5_3:52777556-52777575 None:intergenic 30.0%
GTTGAAATTGATAGACATGT+TGG - chr5_3:52777749-52777768 Msa0817470:five_prime_UTR 30.0%
ATATTACTCTACACTGTGGT+AGG + chr5_3:52775115-52775134 None:intergenic 35.0%
CTATCTTCATCAAACTTTGG+TGG - chr5_3:52775197-52775216 Msa0817470:three_prime_UTR 35.0%
! TCTTGGTTGTTTCAAAATGC+AGG - chr5_3:52775267-52775286 Msa0817470:three_prime_UTR 35.0%
TAGACAACAATGTATGTCAC+CGG - chr5_3:52775323-52775342 Msa0817470:three_prime_UTR 35.0%
AATGAACGATGTTCTAACTC+CGG - chr5_3:52775349-52775368 Msa0817470:three_prime_UTR 35.0%
TTTGATCCTCCTCATTTAAC+CGG + chr5_3:52775378-52775397 None:intergenic 35.0%
ATCTGAATCAGAATGTTCAG+TGG - chr5_3:52775459-52775478 Msa0817470:CDS 35.0%
TGTAGAGGTTTCTGATGTTA+AGG - chr5_3:52775501-52775520 Msa0817470:CDS 35.0%
TTCTGATGTTAAGGTGCATT+TGG - chr5_3:52775510-52775529 Msa0817470:CDS 35.0%
TGTTAAGGTGCATTTGGATA+AGG - chr5_3:52775516-52775535 Msa0817470:CDS 35.0%
! TCATGAATCTGGAATTCCTA+TGG - chr5_3:52775585-52775604 Msa0817470:CDS 35.0%
! AGTATTAGTACACACCCTTT+TGG - chr5_3:52775693-52775712 Msa0817470:intron 35.0%
! AAATCGATTCACTTCTGTGA+CGG + chr5_3:52775868-52775887 None:intergenic 35.0%
!! GGTAAAATTGATTTGTGGAC+CGG - chr5_3:52776004-52776023 Msa0817470:intron 35.0%
TACTAACCCAAACATGATTC+TGG - chr5_3:52776063-52776082 Msa0817470:intron 35.0%
ACTAACCCAAACATGATTCT+GGG - chr5_3:52776064-52776083 Msa0817470:intron 35.0%
GAAGTGAGAGATACATATAC+CGG - chr5_3:52776115-52776134 Msa0817470:intron 35.0%
! CGATGATCCTATAGTGTTTT+AGG - chr5_3:52776211-52776230 Msa0817470:intron 35.0%
TTGTGGAAAAGTTGTGGATA+AGG - chr5_3:52776254-52776273 Msa0817470:intron 35.0%
GGCAGCAAATGAATATACTA+AGG + chr5_3:52776333-52776352 None:intergenic 35.0%
! TCATTTGCTGCCAAATACTT+AGG - chr5_3:52776341-52776360 Msa0817470:intron 35.0%
GCCAAATACTTAGGATAATG+CGG - chr5_3:52776350-52776369 Msa0817470:intron 35.0%
ACCGCATTATCCTAAGTATT+TGG + chr5_3:52776354-52776373 None:intergenic 35.0%
ATCCTAGTTGGGATGTAAAT+GGG + chr5_3:52776544-52776563 None:intergenic 35.0%
AATCCTAGTTGGGATGTAAA+TGG + chr5_3:52776545-52776564 None:intergenic 35.0%
ACTATGCTTGAATCCTAGTT+GGG + chr5_3:52776555-52776574 None:intergenic 35.0%
AACTATGCTTGAATCCTAGT+TGG + chr5_3:52776556-52776575 None:intergenic 35.0%
! CTGCTAAGAAGTATGAAACT+TGG - chr5_3:52776750-52776769 Msa0817470:intron 35.0%
TGTGTTTCAATTACTTCTCG+AGG - chr5_3:52776851-52776870 Msa0817470:intron 35.0%
TTTCACTATAGGAGCTACTA+AGG - chr5_3:52776918-52776937 Msa0817470:intron 35.0%
TCATTAGATATACGCGCATT+TGG + chr5_3:52776993-52777012 None:intergenic 35.0%
! GCGAATAAAGTGTCAAACTT+AGG + chr5_3:52777140-52777159 None:intergenic 35.0%
AACAGAACTTGTGAAATCTG+TGG - chr5_3:52777230-52777249 Msa0817470:CDS 35.0%
CAAAAATTCCGAGTTTCTGA+TGG + chr5_3:52777359-52777378 None:intergenic 35.0%
! CATCAGAAACTCGGAATTTT+TGG - chr5_3:52777357-52777376 Msa0817470:CDS 35.0%
GAATTTCCTTCTAGCATGTA+TGG - chr5_3:52777397-52777416 Msa0817470:CDS 35.0%
CAAAAACCATACATGCTAGA+AGG + chr5_3:52777406-52777425 None:intergenic 35.0%
AGTTACACAGGAATTCAAGA+GGG + chr5_3:52777464-52777483 None:intergenic 35.0%
AAGTTACACAGGAATTCAAG+AGG + chr5_3:52777465-52777484 None:intergenic 35.0%
TTGAATTCCTGTGTAACTTG+AGG - chr5_3:52777466-52777485 Msa0817470:CDS 35.0%
TGAAGCAAGTAACATGATAG+AGG - chr5_3:52777490-52777509 Msa0817470:CDS 35.0%
GAAGCAAGTAACATGATAGA+GGG - chr5_3:52777491-52777510 Msa0817470:CDS 35.0%
TCAAACTATGGAGAGACATA+AGG + chr5_3:52777544-52777563 None:intergenic 35.0%
TGTCTCTCCATAGTTTGATT+TGG - chr5_3:52777546-52777565 Msa0817470:CDS 35.0%
!!! TCTCTTGTTTTGTTCAACCT+AGG - chr5_3:52777630-52777649 Msa0817470:five_prime_UTR 35.0%
TCAACCTAGGATAGTATCAA+AGG - chr5_3:52777643-52777662 Msa0817470:five_prime_UTR 35.0%
ACATCCTTTGATACTATCCT+AGG + chr5_3:52777650-52777669 None:intergenic 35.0%
CATCTCTCTCTCTCTCTATA+GGG - chr5_3:52775056-52775075 Msa0817470:three_prime_UTR 40.0%
GGACAAGTTTCATGTTGTTG+TGG - chr5_3:52775297-52775316 Msa0817470:three_prime_UTR 40.0%
TAGAACATCGTTCATTGCTC+CGG + chr5_3:52775345-52775364 None:intergenic 40.0%
GGTTAAATGAGGAGGATCAA+AGG - chr5_3:52775377-52775396 Msa0817470:three_prime_UTR 40.0%
! GATCAAAGGTGTGAGATTGT+AGG - chr5_3:52775391-52775410 Msa0817470:exon 40.0%
ATCAGAATGTTCAGTGGAGT+TGG - chr5_3:52775465-52775484 Msa0817470:CDS 40.0%
TAAGGTGCATTTGGATAAGG+TGG - chr5_3:52775519-52775538 Msa0817470:CDS 40.0%
CAAAAACATCCAAGCTCCAT+AGG + chr5_3:52775604-52775623 None:intergenic 40.0%
! GAGGCTTGGTTCAAGATTAA+AGG - chr5_3:52775661-52775680 Msa0817470:intron 40.0%
TTCCTCCCAGAATCATGTTT+GGG + chr5_3:52776072-52776091 None:intergenic 40.0%
CAGTCTTTAGATCACTTGTG+TGG - chr5_3:52776171-52776190 Msa0817470:intron 40.0%
ATCCCATTTACATCCCAACT+AGG - chr5_3:52776539-52776558 Msa0817470:intron 40.0%
TCAATTGCACACAATGTAGC+CGG - chr5_3:52777254-52777273 Msa0817470:CDS 40.0%
CATTTGAACTTCCATAGCAC+CGG + chr5_3:52777276-52777295 None:intergenic 40.0%
AAATTCCGAGTTTCTGATGG+TGG + chr5_3:52777356-52777375 None:intergenic 40.0%
!!! AGAAACTCGGAATTTTTGGC+AGG - chr5_3:52777361-52777380 Msa0817470:CDS 40.0%
!!! GAAACTCGGAATTTTTGGCA+GGG - chr5_3:52777362-52777381 Msa0817470:CDS 40.0%
TGATAGAGGGTTATGAACAC+AGG - chr5_3:52777504-52777523 Msa0817470:CDS 40.0%
TGATAGACATGTTGGACATG+TGG - chr5_3:52777757-52777776 Msa0817470:five_prime_UTR 40.0%
GCATCTCTCTCTCTCTCTAT+AGG - chr5_3:52775055-52775074 Msa0817470:three_prime_UTR 45.0%
! GGAGTGTCTGAGAAACAAAG+AGG - chr5_3:52775412-52775431 Msa0817470:CDS 45.0%
ATGTTCAGTGGAGTTGGATC+TGG - chr5_3:52775471-52775490 Msa0817470:CDS 45.0%
TGCATTTGGATAAGGTGGAG+AGG - chr5_3:52775524-52775543 Msa0817470:CDS 45.0%
GCATTTGGATAAGGTGGAGA+GGG - chr5_3:52775525-52775544 Msa0817470:CDS 45.0%
ATAAGGTGGAGAGGGATTGT+AGG - chr5_3:52775533-52775552 Msa0817470:CDS 45.0%
! TCTGGAATTCCTATGGAGCT+TGG - chr5_3:52775592-52775611 Msa0817470:CDS 45.0%
! TGCTCATAAGCATTGTGCTG+AGG - chr5_3:52775642-52775661 Msa0817470:intron 45.0%
! ATAAGCATTGTGCTGAGGCT+TGG - chr5_3:52775647-52775666 Msa0817470:intron 45.0%
AACCCAAACATGATTCTGGG+AGG - chr5_3:52776067-52776086 Msa0817470:intron 45.0%
GTTCCTCCCAGAATCATGTT+TGG + chr5_3:52776073-52776092 None:intergenic 45.0%
! GATTCTGGGAGGAACAAACA+TGG - chr5_3:52776078-52776097 Msa0817470:intron 45.0%
CTTCACCACCATCAGAAACT+CGG - chr5_3:52777348-52777367 Msa0817470:CDS 45.0%
!!! GGAATTTTTGGCAGGGACAT+CGG - chr5_3:52777369-52777388 Msa0817470:CDS 45.0%
GCTTCATCCTCAAGTTACAC+AGG + chr5_3:52777476-52777495 None:intergenic 45.0%
TAGACATGTTGGACATGTGG+AGG - chr5_3:52777760-52777779 Msa0817470:five_prime_UTR 45.0%
GATGTTCTAACTCCGGTGAC+CGG - chr5_3:52775356-52775375 Msa0817470:three_prime_UTR 50.0%
CTCCTCATTTAACCGGTCAC+CGG + chr5_3:52775371-52775390 None:intergenic 50.0%
TCAGTGGAGTTGGATCTGGA+TGG - chr5_3:52775475-52775494 Msa0817470:CDS 50.0%
! ACACAATGTAGCCGGTGCTA+TGG - chr5_3:52777262-52777281 Msa0817470:CDS 50.0%
!! CAGTTGGTGGTACAATGGAG+GGG + chr5_3:52777325-52777344 None:intergenic 50.0%
!! CCAGTTGGTGGTACAATGGA+GGG + chr5_3:52777326-52777345 None:intergenic 50.0%
!! TCCAGTTGGTGGTACAATGG+AGG + chr5_3:52777327-52777346 None:intergenic 50.0%
!! AGGTCCAGTTGGTGGTACAA+TGG + chr5_3:52777330-52777349 None:intergenic 50.0%
!! CGAGTTTCTGATGGTGGTGA+AGG + chr5_3:52777350-52777369 None:intergenic 50.0%
CTCCGGTGACCGGTTAAATG+AGG - chr5_3:52775366-52775385 Msa0817470:three_prime_UTR 55.0%
CGGTGACCGGTTAAATGAGG+AGG - chr5_3:52775369-52775388 Msa0817470:three_prime_UTR 55.0%
GTGGAGTTGGATCTGGATGG+TGG - chr5_3:52775478-52775497 Msa0817470:CDS 55.0%
! GGATCTGGATGGTGGTGTAG+AGG - chr5_3:52775486-52775505 Msa0817470:CDS 55.0%
CCCTCCATTGTACCACCAAC+TGG - chr5_3:52777323-52777342 Msa0817470:CDS 55.0%
! GATGGTGGTGAAGGTCCAGT+TGG + chr5_3:52777341-52777360 None:intergenic 55.0%
GGTGGTGAAGGTCCAGTTGG+TGG + chr5_3:52777338-52777357 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 52775029 52777846 52775029 ID=Msa0817470;Name=Msa0817470
chr5_3 mRNA 52775029 52777846 52775029 ID=Msa0817470-mRNA-1;Parent=Msa0817470;Name=Msa0817470-mRNA-1;_AED=0.00;_eAED=0.00;_QI=254|1|1|1|1|1|2|377|213
chr5_3 exon 52775029 52775640 52775029 ID=Msa0817470-mRNA-1:exon:15858;Parent=Msa0817470-mRNA-1
chr5_3 exon 52777186 52777846 52777186 ID=Msa0817470-mRNA-1:exon:15857;Parent=Msa0817470-mRNA-1
chr5_3 five_prime_UTR 52777593 52777846 52777593 ID=Msa0817470-mRNA-1:five_prime_utr;Parent=Msa0817470-mRNA-1
chr5_3 CDS 52777186 52777592 52777186 ID=Msa0817470-mRNA-1:cds;Parent=Msa0817470-mRNA-1
chr5_3 CDS 52775406 52775640 52775406 ID=Msa0817470-mRNA-1:cds;Parent=Msa0817470-mRNA-1
chr5_3 three_prime_UTR 52775029 52775405 52775029 ID=Msa0817470-mRNA-1:three_prime_utr;Parent=Msa0817470-mRNA-1
Gene Sequence

>Msa0817470

ATGCAGGTAAATGAGGACAAGTTTCATGTTGTTGTGGACATAGACAACAATGTATGTCACCGGAGCAATGAACGATGTTCTAACTCCGGTGACCGGTTAAATGAGGAGGATCAAAGGTGTGAGATTGTAGGAGTGTCTGAGAAACAAAGAGGATCTTGTGATTCAGATGATAATTCATCTGAATCAGAATGTTCAGTGGAGTTGGATCTGGATGGTGGTGTAGAGGTTTCTGATGTTAAGGTGCATTTGGATAAGGTGGAGAGGGATTGTAGGATTTGTCATCTTAGTATGGATATGACAAATCATGAATCTGGAATTCCTATGGAGCTTGGATGTTTTTGCAAAAATGATTTGGCTGCTGCTCATAAGCATTGTGCTGAGGCTTGGTTCAAGATTAAAGGCAATAAAACTTGTGAAATCTGTGGATCAATTGCACACAATGTAGCCGGTGCTATGGAAGTTCAAATGACAGAACAATTTAATGAATCAAGTGACCCCTCCATTGTACCACCAACTGGACCTTCACCACCATCAGAAACTCGGAATTTTTGGCAGGGACATCGGTTTTTGAATTTCCTTCTAGCATGTATGGTTTTTGCCTTTGTCATATCATGGCTTTTTCACTTTAATGTTCCCTCTTGA
Protein sequence

>Msa0817470

MQVNEDKFHVVVDIDNNVCHRSNERCSNSGDRLNEEDQRCEIVGVSEKQRGSCDSDDNSSESECSVELDLDGGVEVSDVKVHLDKVERDCRICHLSMDMTNHESGIPMELGCFCKNDLAAAHKHCAEAWFKIKGNKTCEICGSIAHNVAGAMEVQMTEQFNESSDPSIVPPTGPSPPSETRNFWQGHRFLNFLLACMVFAFVISWLFHFNVPS*