AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0825250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825250 MtrunA17_Chr5g0446831 99.020 204 2 0 1 204 1 204 2.13e-148 410
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825250 AT5G55940.1 71.845 206 56 2 1 204 3 208 3.20e-107 306
Msa0825250 AT5G51620.4 45.714 105 42 2 70 171 1 93 4.48e-22 88.6
Msa0825250 AT5G51620.3 76.000 50 12 0 70 119 1 50 4.83e-22 85.1
Msa0825250 AT5G51620.1 71.698 53 15 0 70 122 1 53 4.41e-21 84.7
Msa0825250 AT5G51620.2 49.206 63 32 0 70 132 1 63 2.20e-12 60.8

Find 71 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATGTAGGGATATTAATT+TGG 0.219499 5_3:+65005195 Msa0825250:three_prime_UTR
AAACTCTGAAAATTGTTAAT+TGG 0.225404 5_3:-65005110 None:intergenic
ATCAGCAAATGTGATCTTAT+TGG 0.252981 5_3:+65004702 Msa0825250:CDS
TTATGGTTTAGCTTTATGTT+AGG 0.282779 5_3:+65004614 Msa0825250:intron
ATTGGAGATCTCTCTCATTC+TGG 0.297316 5_3:+65002909 Msa0825250:CDS
TCCACAAGCTGCTATTCTAT+TGG 0.321743 5_3:+65003541 Msa0825250:CDS
ATCCATTATCCTGTTTCATA+TGG 0.347732 5_3:+65005275 Msa0825250:three_prime_UTR
AATCCCTCTTCTTCCTCAAT+TGG 0.350775 5_3:+65002891 Msa0825250:CDS
AACCATATGAAACAGGATAA+TGG 0.358913 5_3:-65005277 None:intergenic
TATGGTTTAGCTTTATGTTA+GGG 0.373331 5_3:+65004615 Msa0825250:intron
ACTATATTTCATCTGAGAAA+TGG 0.381066 5_3:+65004725 Msa0825250:CDS
GGGATTTGAGAGTGGAATAG+AGG 0.397989 5_3:-65002874 None:intergenic
AATTCTTGTAACGAACAATC+AGG 0.409195 5_3:+65005015 Msa0825250:three_prime_UTR
ACCAACTATGTTCAACCCTT+TGG 0.413858 5_3:-65003435 None:intergenic
CACCCACTTGCAAAGTAGAT+TGG 0.423722 5_3:-65004962 None:intergenic
CAAAGTAGATTGGATACAAA+TGG 0.423795 5_3:-65004952 None:intergenic
ACTTCCCATGCTTCTGATGA+TGG 0.424911 5_3:-65002707 None:intergenic
CAGAGTTTCCCTTCCACAAA+GGG 0.426807 5_3:+65005125 Msa0825250:three_prime_UTR
ACCAAAGGGTTGAACATAGT+TGG 0.427209 5_3:+65003434 Msa0825250:CDS
CTTAGCAAACCATATGAAAC+AGG 0.429072 5_3:-65005284 None:intergenic
TTAGGGATGCATCTAAGAAC+TGG 0.438779 5_3:+65004632 Msa0825250:CDS
GATTTCAACGATTTCGTTGG+AGG 0.453950 5_3:-65002839 None:intergenic
CGAGATCAGTTGAAGAGCCC+TGG 0.457489 5_3:-65004920 None:intergenic
ACGCCGTTGACGGCGGATGT+AGG 0.461476 5_3:-65002796 None:intergenic
CGCCGTTGACGGCGGATGTA+GGG 0.461819 5_3:-65002795 None:intergenic
GCCGATCAAGACGCCGTTGA+CGG 0.463051 5_3:-65002806 None:intergenic
TCCCTACATAAAAGTTAGAA+AGG 0.473936 5_3:-65005184 None:intergenic
GCCGTCAACGGCGTCTTGAT+CGG 0.478430 5_3:+65002805 Msa0825250:CDS
CTGTTTCATATGGTTTGCTA+AGG 0.482939 5_3:+65005285 Msa0825250:three_prime_UTR
GGATTTGAGAGTGGAATAGA+GGG 0.487935 5_3:-65002873 None:intergenic
AAAATCATCAGACCTCTCAT+TGG 0.502605 5_3:-65003468 None:intergenic
ATCTCCAATTGAGGAAGAAG+AGG 0.512179 5_3:-65002895 None:intergenic
ATGGCTCTTTGAGAGAGAAT+CGG 0.512595 5_3:-65004681 None:intergenic
GGTGATTTCAACGATTTCGT+TGG 0.519237 5_3:-65002842 None:intergenic
TTCAACGATTTCGTTGGAGG+AGG 0.520719 5_3:-65002836 None:intergenic
AAAATATGGCCCTTTGTGGA+AGG 0.526000 5_3:-65005134 None:intergenic
TCAGGGATTGGCTAAACCCA+GGG 0.530286 5_3:+65004903 Msa0825250:intron
TGCATCTAAGAACTGGAAGT+TGG 0.530544 5_3:+65004639 Msa0825250:CDS
TTCAGGGATTGGCTAAACCC+AGG 0.536967 5_3:+65004902 Msa0825250:intron
ATCATCCATCATCAGAAGCA+TGG 0.541246 5_3:+65002702 Msa0825250:exon
CCTACTTGCTTATATCATCA+AGG 0.542389 5_3:-65004774 None:intergenic
CACCCTACATCCGCCGTCAA+CGG 0.543335 5_3:+65002793 Msa0825250:CDS
GAAGAAGAGGGATTTGAGAG+TGG 0.543708 5_3:-65002882 None:intergenic
CAGGGCTCTTCAACTGATCT+CGG 0.551747 5_3:+65004921 Msa0825250:exon
AAAGAAAATAGCGCAAAGGA+AGG 0.559428 5_3:-65005358 None:intergenic
TCATCCATCATCAGAAGCAT+GGG 0.563935 5_3:+65002703 Msa0825250:exon
GAGATCAGTTGAAGAGCCCT+GGG 0.564845 5_3:-65004919 None:intergenic
TATCCAATCTACTTTGCAAG+TGG 0.565605 5_3:+65004959 Msa0825250:three_prime_UTR
GTGGAATAGAGGGACGGCAT+CGG 0.567477 5_3:-65002863 None:intergenic
GTGGAAAGTAACGACAGATA+TGG 0.568666 5_3:-65003523 None:intergenic
AAATATGGCCCTTTGTGGAA+GGG 0.568800 5_3:-65005133 None:intergenic
GAGTAGCAGTGCTATAATGC+AGG 0.572270 5_3:+65003686 Msa0825250:CDS
GGTGATGTTCAAGCATATCA+TGG 0.573813 5_3:+65004981 Msa0825250:three_prime_UTR
ACCAATAGAATAGCAGCTTG+TGG 0.585214 5_3:-65003542 None:intergenic
AATAAGATCACATTTGCTGA+TGG 0.585234 5_3:-65004700 None:intergenic
AAATAGCGCAAAGGAAGGAA+AGG 0.588028 5_3:-65005353 None:intergenic
ATCCAATCTACTTTGCAAGT+GGG 0.591536 5_3:+65004960 Msa0825250:three_prime_UTR
ATGAGAGAGATCTCCAATTG+AGG 0.598752 5_3:-65002904 None:intergenic
ACTTTATCAGATGTACAAGC+AGG 0.605745 5_3:+65005076 Msa0825250:three_prime_UTR
TCAGAGTTTCCCTTCCACAA+AGG 0.611334 5_3:+65005124 Msa0825250:three_prime_UTR
ATTCTTGTAACGAACAATCA+GGG 0.613229 5_3:+65005016 Msa0825250:three_prime_UTR
TCTCCAATTGAGGAAGAAGA+GGG 0.626207 5_3:-65002894 None:intergenic
GATCAAGACGCCGTTGACGG+CGG 0.635153 5_3:-65002803 None:intergenic
AGGGAATAGATCTTGACTTG+AGG 0.636762 5_3:+65005035 Msa0825250:three_prime_UTR
ATTGGATACAAATGGACTGA+GGG 0.643356 5_3:-65004944 None:intergenic
GGTGGTGTGGCAAAGAACAT+TGG 0.647028 5_3:+65003497 Msa0825250:CDS
TTGAGAGTGGAATAGAGGGA+CGG 0.654117 5_3:-65002869 None:intergenic
AAGTAACGATTTGAAGTACG+AGG 0.660885 5_3:+65002726 Msa0825250:CDS
TTTCGTTGGAGGAGGAGATG+CGG 0.668526 5_3:-65002828 None:intergenic
TTCACAACACAGTCACACGA+AGG 0.671673 5_3:-65002674 None:intergenic
GATTGGATACAAATGGACTG+AGG 0.671901 5_3:-65004945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTGTTTTATGAATTTTATTT+GGG + chr5_3:65003756-65003775 Msa0825250:intron 15.0%
!! TTTAATAACAAAAAAAGGAA+AGG - chr5_3:65004466-65004485 None:intergenic 15.0%
!!! TTTTAGACTTATATTTTCTA+AGG + chr5_3:65005320-65005339 Msa0825250:three_prime_UTR 15.0%
!!! ATTTAGCAGATAACAATTTT+GGG - chr5_3:65003028-65003047 None:intergenic 20.0%
!!! AATTTAGCAGATAACAATTT+TGG - chr5_3:65003029-65003048 None:intergenic 20.0%
!!! AATTAGTGAATTTTCATGTT+TGG + chr5_3:65003194-65003213 Msa0825250:intron 20.0%
!! AAAATTGCAATCATATTTGT+TGG - chr5_3:65003262-65003281 None:intergenic 20.0%
!!! GCTGTTTTATGAATTTTATT+TGG + chr5_3:65003755-65003774 Msa0825250:intron 20.0%
!! TTCACAGTAAATATAGAATT+CGG + chr5_3:65003922-65003941 Msa0825250:intron 20.0%
!!! AAAGAACTCCTTTTTTATAA+TGG + chr5_3:65004031-65004050 Msa0825250:intron 20.0%
!! CAATGATTCCATTATAAAAA+AGG - chr5_3:65004042-65004061 None:intergenic 20.0%
!! AATCAAGAAGAAAAAAGATT+GGG - chr5_3:65004828-65004847 None:intergenic 20.0%
!! AAATCAAGAAGAAAAAAGAT+TGG - chr5_3:65004829-65004848 None:intergenic 20.0%
!!! TATTATCCAATTTTGTTTTC+AGG + chr5_3:65004885-65004904 Msa0825250:intron 20.0%
!!! ATTATCCAATTTTGTTTTCA+GGG + chr5_3:65004886-65004905 Msa0825250:intron 20.0%
!!! AAACTCTGAAAATTGTTAAT+TGG - chr5_3:65005113-65005132 None:intergenic 20.0%
!! TTTATGTAGGGATATTAATT+TGG + chr5_3:65005195-65005214 Msa0825250:three_prime_UTR 20.0%
! TAACATGCAATTTAATCATC+TGG + chr5_3:65003076-65003095 Msa0825250:intron 25.0%
! AACATGCAATTTAATCATCT+GGG + chr5_3:65003077-65003096 Msa0825250:intron 25.0%
! ACAAAAAAATCAGAAAACTG+TGG - chr5_3:65003366-65003385 None:intergenic 25.0%
! TCTAGATAATGTTCAATCAA+GGG + chr5_3:65003784-65003803 Msa0825250:intron 25.0%
! TCACAGTAAATATAGAATTC+GGG + chr5_3:65003923-65003942 Msa0825250:intron 25.0%
! CAAATATCTAGAAATCCTTT+AGG + chr5_3:65003990-65004009 Msa0825250:intron 25.0%
! ATACGCATATTATAGTTCAA+AGG - chr5_3:65004068-65004087 None:intergenic 25.0%
!!! TTTTGACTTATGCTGAAAAT+AGG - chr5_3:65004205-65004224 None:intergenic 25.0%
!!! GATCTAAAATTTTGATGAGA+TGG + chr5_3:65004269-65004288 Msa0825250:intron 25.0%
! CTTACTCTGCTTAAATATAT+AGG + chr5_3:65004321-65004340 Msa0825250:intron 25.0%
! TTTGTAATCAGTACAAACAT+TGG - chr5_3:65004377-65004396 None:intergenic 25.0%
! TAACAAAAAAAGGAAAGGAA+AGG - chr5_3:65004461-65004480 None:intergenic 25.0%
!!! AAAGATTGCCTTTTTTCTTA+TGG + chr5_3:65004597-65004616 Msa0825250:intron 25.0%
! AAGCTAAACCATAAGAAAAA+AGG - chr5_3:65004608-65004627 None:intergenic 25.0%
!!! TTATGGTTTAGCTTTATGTT+AGG + chr5_3:65004614-65004633 Msa0825250:intron 25.0%
!!! TATGGTTTAGCTTTATGTTA+GGG + chr5_3:65004615-65004634 Msa0825250:intron 25.0%
! ACTATATTTCATCTGAGAAA+TGG + chr5_3:65004725-65004744 Msa0825250:CDS 25.0%
!! TGTAAAGTTGATTATAACTG+CGG - chr5_3:65004858-65004877 None:intergenic 25.0%
!! TTCCTTTCTAACTTTTATGT+AGG + chr5_3:65005182-65005201 Msa0825250:three_prime_UTR 25.0%
!! TCCTTTCTAACTTTTATGTA+GGG + chr5_3:65005183-65005202 Msa0825250:three_prime_UTR 25.0%
!! TGTTTGTGTTTGTGTTTTTG+TGG - chr5_3:65002639-65002658 None:intergenic 30.0%
! AGGAATACTTTTCTACCAAA+GGG + chr5_3:65003420-65003439 Msa0825250:CDS 30.0%
CTCTAGATAATGTTCAATCA+AGG + chr5_3:65003783-65003802 Msa0825250:intron 30.0%
CACAGTAAATATAGAATTCG+GGG + chr5_3:65003924-65003943 Msa0825250:intron 30.0%
! TTTTGCATTTGACAATGTCT+GGG + chr5_3:65004101-65004120 Msa0825250:intron 30.0%
ATCTTGAAATTCAGATCTGA+TGG + chr5_3:65004138-65004157 Msa0825250:intron 30.0%
! TTTTCAGCATAAGTCAAAAG+AGG + chr5_3:65004206-65004225 Msa0825250:intron 30.0%
TTTCAGCATAAGTCAAAAGA+GGG + chr5_3:65004207-65004226 Msa0825250:intron 30.0%
GATCAATATATTGCAATGTG+TGG - chr5_3:65004253-65004272 None:intergenic 30.0%
TGTAATCTCAATCCACTATA+AGG + chr5_3:65004345-65004364 Msa0825250:intron 30.0%
AAAGGAAAGGAAAGGAAAAT+AGG - chr5_3:65004453-65004472 None:intergenic 30.0%
AAAGATGGATCTACATCATA+TGG - chr5_3:65004560-65004579 None:intergenic 30.0%
! ATGTAGATCCATCTTTTTGT+TGG + chr5_3:65004564-65004583 Msa0825250:intron 30.0%
TAATGCAACCAACAAAAAGA+TGG - chr5_3:65004575-65004594 None:intergenic 30.0%
! AATAAGATCACATTTGCTGA+TGG - chr5_3:65004703-65004722 None:intergenic 30.0%
ATCAGCAAATGTGATCTTAT+TGG + chr5_3:65004702-65004721 Msa0825250:CDS 30.0%
AAAAAGATTGGGAGAATGAA+GGG - chr5_3:65004817-65004836 None:intergenic 30.0%
AAAAAAGATTGGGAGAATGA+AGG - chr5_3:65004818-65004837 None:intergenic 30.0%
CAAAGTAGATTGGATACAAA+TGG - chr5_3:65004955-65004974 None:intergenic 30.0%
AATTCTTGTAACGAACAATC+AGG + chr5_3:65005015-65005034 Msa0825250:three_prime_UTR 30.0%
ATTCTTGTAACGAACAATCA+GGG + chr5_3:65005016-65005035 Msa0825250:three_prime_UTR 30.0%
AGCCTTTGCAACAAAAAATA+TGG - chr5_3:65005151-65005170 None:intergenic 30.0%
TCCCTACATAAAAGTTAGAA+AGG - chr5_3:65005187-65005206 None:intergenic 30.0%
ATCCATTATCCTGTTTCATA+TGG + chr5_3:65005275-65005294 Msa0825250:three_prime_UTR 30.0%
AACCATATGAAACAGGATAA+TGG - chr5_3:65005280-65005299 None:intergenic 30.0%
! AAGTAACGATTTGAAGTACG+AGG + chr5_3:65002726-65002745 Msa0825250:CDS 35.0%
!! TTTTGGGTCAGAATTCAAAG+AGG - chr5_3:65003012-65003031 None:intergenic 35.0%
ATGCAATTTAATCATCTGGG+TGG + chr5_3:65003080-65003099 Msa0825250:intron 35.0%
!! GGTGGATCTTTATTTCTGTT+GGG + chr5_3:65003098-65003117 Msa0825250:intron 35.0%
CAACATGTGAGTGTTATGTT+TGG + chr5_3:65003307-65003326 Msa0825250:intron 35.0%
ATTGTCTGTGTGATAGATAG+AGG + chr5_3:65003400-65003419 Msa0825250:intron 35.0%
! GAGGAATACTTTTCTACCAA+AGG + chr5_3:65003419-65003438 Msa0825250:CDS 35.0%
!! GTTATTTTCATGCCAATGAG+AGG + chr5_3:65003456-65003475 Msa0825250:CDS 35.0%
AAAATCATCAGACCTCTCAT+TGG - chr5_3:65003471-65003490 None:intergenic 35.0%
AGTAAATATAGAATTCGGGG+TGG + chr5_3:65003927-65003946 Msa0825250:intron 35.0%
GGTGGATGAGAATCAAATTT+GGG + chr5_3:65003945-65003964 Msa0825250:intron 35.0%
GTGGATGAGAATCAAATTTG+GGG + chr5_3:65003946-65003965 Msa0825250:intron 35.0%
TGGATGAGAATCAAATTTGG+GGG + chr5_3:65003947-65003966 Msa0825250:intron 35.0%
ACATTGTCAAATGCAAAAGG+AGG - chr5_3:65004099-65004118 None:intergenic 35.0%
CAGACATTGTCAAATGCAAA+AGG - chr5_3:65004102-65004121 None:intergenic 35.0%
! CTTTTGCATTTGACAATGTC+TGG + chr5_3:65004100-65004119 Msa0825250:intron 35.0%
ACTTATGCTGAAAATAGGAG+GGG - chr5_3:65004200-65004219 None:intergenic 35.0%
GACTTATGCTGAAAATAGGA+GGG - chr5_3:65004201-65004220 None:intergenic 35.0%
TGACTTATGCTGAAAATAGG+AGG - chr5_3:65004202-65004221 None:intergenic 35.0%
CATTGGATGAAACCTTATAG+TGG - chr5_3:65004360-65004379 None:intergenic 35.0%
GGAATTAAGGCAAGTATTCA+GGG - chr5_3:65004424-65004443 None:intergenic 35.0%
AAAGGAAAGGAAAATAGGAG+GGG - chr5_3:65004448-65004467 None:intergenic 35.0%
GAAAGGAAAGGAAAATAGGA+GGG - chr5_3:65004449-65004468 None:intergenic 35.0%
GGATCTACATCATATGGTAA+AGG - chr5_3:65004554-65004573 None:intergenic 35.0%
CCTACTTGCTTATATCATCA+AGG - chr5_3:65004777-65004796 None:intergenic 35.0%
! CCTTGATGATATAAGCAAGT+AGG + chr5_3:65004774-65004793 Msa0825250:intron 35.0%
CCAATCCCTGAAAACAAAAT+TGG - chr5_3:65004894-65004913 None:intergenic 35.0%
!!! CCAATTTTGTTTTCAGGGAT+TGG + chr5_3:65004891-65004910 Msa0825250:intron 35.0%
!! ATTGGATACAAATGGACTGA+GGG - chr5_3:65004947-65004966 None:intergenic 35.0%
TATCCAATCTACTTTGCAAG+TGG + chr5_3:65004959-65004978 Msa0825250:three_prime_UTR 35.0%
ATCCAATCTACTTTGCAAGT+GGG + chr5_3:65004960-65004979 Msa0825250:three_prime_UTR 35.0%
ACTTTATCAGATGTACAAGC+AGG + chr5_3:65005076-65005095 Msa0825250:three_prime_UTR 35.0%
ACAAAAAATATGGCCCTTTG+TGG - chr5_3:65005141-65005160 None:intergenic 35.0%
!!! GGCCATATTTTTTGTTGCAA+AGG + chr5_3:65005146-65005165 Msa0825250:three_prime_UTR 35.0%
CTTAGCAAACCATATGAAAC+AGG - chr5_3:65005287-65005306 None:intergenic 35.0%
!! CTGTTTCATATGGTTTGCTA+AGG + chr5_3:65005285-65005304 Msa0825250:three_prime_UTR 35.0%
! GTTTTTGTGGACTTCGATGT+TGG - chr5_3:65002626-65002645 None:intergenic 40.0%
ATCATCCATCATCAGAAGCA+TGG + chr5_3:65002702-65002721 Msa0825250:exon 40.0%
! TCATCCATCATCAGAAGCAT+GGG + chr5_3:65002703-65002722 Msa0825250:exon 40.0%
GATTTCAACGATTTCGTTGG+AGG - chr5_3:65002842-65002861 None:intergenic 40.0%
GGTGATTTCAACGATTTCGT+TGG - chr5_3:65002845-65002864 None:intergenic 40.0%
GGATTTGAGAGTGGAATAGA+GGG - chr5_3:65002876-65002895 None:intergenic 40.0%
AATCCCTCTTCTTCCTCAAT+TGG + chr5_3:65002891-65002910 Msa0825250:CDS 40.0%
TCTCCAATTGAGGAAGAAGA+GGG - chr5_3:65002897-65002916 None:intergenic 40.0%
ATCTCCAATTGAGGAAGAAG+AGG - chr5_3:65002898-65002917 None:intergenic 40.0%
ATGAGAGAGATCTCCAATTG+AGG - chr5_3:65002907-65002926 None:intergenic 40.0%
ATTGGAGATCTCTCTCATTC+TGG + chr5_3:65002909-65002928 Msa0825250:CDS 40.0%
!! GGGTGGATCTTTATTTCTGT+TGG + chr5_3:65003097-65003116 Msa0825250:intron 40.0%
! CATATTTGTTGGTGTCCTGT+TGG - chr5_3:65003251-65003270 None:intergenic 40.0%
ACCAAAGGGTTGAACATAGT+TGG + chr5_3:65003434-65003453 Msa0825250:CDS 40.0%
ACCAACTATGTTCAACCCTT+TGG - chr5_3:65003438-65003457 None:intergenic 40.0%
! AGGTCTGATGATTTTGAGCT+TGG + chr5_3:65003476-65003495 Msa0825250:CDS 40.0%
! TCTGATGATTTTGAGCTTGG+TGG + chr5_3:65003479-65003498 Msa0825250:CDS 40.0%
GTGGAAAGTAACGACAGATA+TGG - chr5_3:65003526-65003545 None:intergenic 40.0%
TCCACAAGCTGCTATTCTAT+TGG + chr5_3:65003541-65003560 Msa0825250:CDS 40.0%
ACCAATAGAATAGCAGCTTG+TGG - chr5_3:65003545-65003564 None:intergenic 40.0%
GGGTGGATGAGAATCAAATT+TGG + chr5_3:65003944-65003963 Msa0825250:intron 40.0%
! ATTTGGGGGTGAATTTTGTC+TGG + chr5_3:65003961-65003980 Msa0825250:intron 40.0%
! TGGCAAGTCTTATAATCACG+AGG + chr5_3:65004158-65004177 Msa0825250:intron 40.0%
CTTATGCTGAAAATAGGAGG+GGG - chr5_3:65004199-65004218 None:intergenic 40.0%
ATTCAGGGCTATATGGAAGA+CGG - chr5_3:65004409-65004428 None:intergenic 40.0%
GGGAATTAAGGCAAGTATTC+AGG - chr5_3:65004425-65004444 None:intergenic 40.0%
AAGGAAAGGAAAATAGGAGG+GGG - chr5_3:65004447-65004466 None:intergenic 40.0%
GGAAAGGAAAGGAAAATAGG+AGG - chr5_3:65004450-65004469 None:intergenic 40.0%
TTAGGGATGCATCTAAGAAC+TGG + chr5_3:65004632-65004651 Msa0825250:CDS 40.0%
TGCATCTAAGAACTGGAAGT+TGG + chr5_3:65004639-65004658 Msa0825250:CDS 40.0%
! ATGGCTCTTTGAGAGAGAAT+CGG - chr5_3:65004684-65004703 None:intergenic 40.0%
!! GATTGGATACAAATGGACTG+AGG - chr5_3:65004948-65004967 None:intergenic 40.0%
GGTGATGTTCAAGCATATCA+TGG + chr5_3:65004981-65005000 Msa0825250:three_prime_UTR 40.0%
! AGGGAATAGATCTTGACTTG+AGG + chr5_3:65005035-65005054 Msa0825250:three_prime_UTR 40.0%
AAATATGGCCCTTTGTGGAA+GGG - chr5_3:65005136-65005155 None:intergenic 40.0%
AAAATATGGCCCTTTGTGGA+AGG - chr5_3:65005137-65005156 None:intergenic 40.0%
! TGGAGTTTTCTTGCTTTGCT+TGG + chr5_3:65005215-65005234 Msa0825250:three_prime_UTR 40.0%
TTCACAACACAGTCACACGA+AGG - chr5_3:65002677-65002696 None:intergenic 45.0%
ACTTCCCATGCTTCTGATGA+TGG - chr5_3:65002710-65002729 None:intergenic 45.0%
TTCAACGATTTCGTTGGAGG+AGG - chr5_3:65002839-65002858 None:intergenic 45.0%
TTGAGAGTGGAATAGAGGGA+CGG - chr5_3:65002872-65002891 None:intergenic 45.0%
GGGATTTGAGAGTGGAATAG+AGG - chr5_3:65002877-65002896 None:intergenic 45.0%
GAAGAAGAGGGATTTGAGAG+TGG - chr5_3:65002885-65002904 None:intergenic 45.0%
GAGATCTCTCTCATTCTGGT+TGG + chr5_3:65002913-65002932 Msa0825250:intron 45.0%
TCTCTCTCTCTCTCTCTCTT+AGG + chr5_3:65002970-65002989 Msa0825250:intron 45.0%
CTCTCTCTCTCTCTCTCTTA+GGG + chr5_3:65002971-65002990 Msa0825250:intron 45.0%
!!! TGATTTTGAGCTTGGTGGTG+TGG + chr5_3:65003484-65003503 Msa0825250:CDS 45.0%
GAGTAGCAGTGCTATAATGC+AGG + chr5_3:65003686-65003705 Msa0825250:CDS 45.0%
!! TGTTCAATCAAGGGAGCGTT+TGG + chr5_3:65003793-65003812 Msa0825250:intron 45.0%
!! GTTCAATCAAGGGAGCGTTT+GGG + chr5_3:65003794-65003813 Msa0825250:intron 45.0%
GGAGGGGGAATTAAGTATTC+AGG - chr5_3:65004184-65004203 None:intergenic 45.0%
GGCAAGTATTCAGGGCTATA+TGG - chr5_3:65004416-65004435 None:intergenic 45.0%
AAATAGGAGGGGGGGAATTA+AGG - chr5_3:65004437-65004456 None:intergenic 45.0%
AGGAAAGGAAAATAGGAGGG+GGG - chr5_3:65004446-65004465 None:intergenic 45.0%
ATTGGGAGAATGAAGGGTAG+CGG - chr5_3:65004811-65004830 None:intergenic 45.0%
CACCCACTTGCAAAGTAGAT+TGG - chr5_3:65004965-65004984 None:intergenic 45.0%
TCAGAGTTTCCCTTCCACAA+AGG + chr5_3:65005124-65005143 Msa0825250:three_prime_UTR 45.0%
CAGAGTTTCCCTTCCACAAA+GGG + chr5_3:65005125-65005144 Msa0825250:three_prime_UTR 45.0%
!!! TTTATTTTAATAACAAAAAA+AGG - chr5_3:65004471-65004490 None:intergenic 5.0%
TTTCGTTGGAGGAGGAGATG+CGG - chr5_3:65002831-65002850 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+TGG - chr5_3:65002940-65002959 None:intergenic 50.0%
GTGTTGTGTTCGACACCAAC+AGG + chr5_3:65003233-65003252 Msa0825250:intron 50.0%
GGTGGTGTGGCAAAGAACAT+TGG + chr5_3:65003497-65003516 Msa0825250:CDS 50.0%
CTGCGCAGGATAAGTCCTAA+AGG - chr5_3:65004008-65004027 None:intergenic 50.0%
AGGAGTTCTTTGATCTGCGC+AGG - chr5_3:65004022-65004041 None:intergenic 50.0%
GGAAAGGAAAATAGGAGGGG+GGG - chr5_3:65004445-65004464 None:intergenic 50.0%
!! TTCAGGGATTGGCTAAACCC+AGG + chr5_3:65004902-65004921 Msa0825250:intron 50.0%
!! TCAGGGATTGGCTAAACCCA+GGG + chr5_3:65004903-65004922 Msa0825250:intron 50.0%
GAGATCAGTTGAAGAGCCCT+GGG - chr5_3:65004922-65004941 None:intergenic 50.0%
CAGGGCTCTTCAACTGATCT+CGG + chr5_3:65004921-65004940 Msa0825250:exon 50.0%
GTGGAATAGAGGGACGGCAT+CGG - chr5_3:65002866-65002885 None:intergenic 55.0%
GGGCTATATGGAAGACGGCA+TGG - chr5_3:65004404-65004423 None:intergenic 55.0%
CGAGATCAGTTGAAGAGCCC+TGG - chr5_3:65004923-65004942 None:intergenic 55.0%
CACCCTACATCCGCCGTCAA+CGG + chr5_3:65002793-65002812 Msa0825250:CDS 60.0%
GATCAAGACGCCGTTGACGG+CGG - chr5_3:65002806-65002825 None:intergenic 60.0%
GCCGTCAACGGCGTCTTGAT+CGG + chr5_3:65002805-65002824 Msa0825250:CDS 60.0%
GCCGATCAAGACGCCGTTGA+CGG - chr5_3:65002809-65002828 None:intergenic 60.0%
! GGGAGAATGAAGGGTAGCGG+TGG - chr5_3:65004808-65004827 None:intergenic 60.0%
CGCCGTTGACGGCGGATGTA+GGG - chr5_3:65002798-65002817 None:intergenic 65.0%
ACGCCGTTGACGGCGGATGT+AGG - chr5_3:65002799-65002818 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 65002608 65005368 65002608 ID=Msa0825250;Name=Msa0825250
chr5_3 mRNA 65002608 65005368 65002608 ID=Msa0825250-mRNA-1;Parent=Msa0825250;Name=Msa0825250-mRNA-1;_AED=0.04;_eAED=0.04;_QI=113|1|1|1|1|1|5|431|204
chr5_3 exon 65002608 65002930 65002608 ID=Msa0825250-mRNA-1:exon:20340;Parent=Msa0825250-mRNA-1
chr5_3 exon 65003416 65003562 65003416 ID=Msa0825250-mRNA-1:exon:20341;Parent=Msa0825250-mRNA-1
chr5_3 exon 65003648 65003707 65003648 ID=Msa0825250-mRNA-1:exon:20342;Parent=Msa0825250-mRNA-1
chr5_3 exon 65004625 65004791 65004625 ID=Msa0825250-mRNA-1:exon:20343;Parent=Msa0825250-mRNA-1
chr5_3 exon 65004907 65005368 65004907 ID=Msa0825250-mRNA-1:exon:20344;Parent=Msa0825250-mRNA-1
chr5_3 five_prime_UTR 65002608 65002720 65002608 ID=Msa0825250-mRNA-1:five_prime_utr;Parent=Msa0825250-mRNA-1
chr5_3 CDS 65002721 65002930 65002721 ID=Msa0825250-mRNA-1:cds;Parent=Msa0825250-mRNA-1
chr5_3 CDS 65003416 65003562 65003416 ID=Msa0825250-mRNA-1:cds;Parent=Msa0825250-mRNA-1
chr5_3 CDS 65003648 65003707 65003648 ID=Msa0825250-mRNA-1:cds;Parent=Msa0825250-mRNA-1
chr5_3 CDS 65004625 65004791 65004625 ID=Msa0825250-mRNA-1:cds;Parent=Msa0825250-mRNA-1
chr5_3 CDS 65004907 65004937 65004907 ID=Msa0825250-mRNA-1:cds;Parent=Msa0825250-mRNA-1
chr5_3 three_prime_UTR 65004938 65005368 65004938 ID=Msa0825250-mRNA-1:three_prime_utr;Parent=Msa0825250-mRNA-1
Gene Sequence

>Msa0825250

ATGGGAAGTAACGATTTGAAGTACGAGGTTTCACAAAACGCATACATCAAACTCGTTCTTCACTCTCTCAAACACCCTACATCCGCCGTCAACGGCGTCTTGATCGGCCGCATCTCCTCCTCCAACGAAATCGTTGAAATCACCGATGCCGTCCCTCTATTCCACTCTCAAATCCCTCTTCTTCCTCAATTGGAGATCTCTCTCATTCTGATAGAGGAATACTTTTCTACCAAAGGGTTGAACATAGTTGGTTATTTTCATGCCAATGAGAGGTCTGATGATTTTGAGCTTGGTGGTGTGGCAAAGAACATTGGTGACCATATCTGTCGTTACTTTCCACAAGCTGCTATTCTATTGTTAGATAACAAGAAGCTTGAAGCTTTGAAAAAGAGCAAGAGTAGCAGTGCTATAATGCAGCTTTATGTTAGGGATGCATCTAAGAACTGGAAGTTGGTTCAGTCAGATGCAAACAACCGATTCTCTCTCAAAGAGCCATCAGCAAATGTGATCTTATTGGACTATATTTCATCTGAGAAATGGAATGACATTGTAGATTTTGATGATCACCTTGATGATATAAGCAAGGATTGGCTAAACCCAGGGCTCTTCAACTGA
Protein sequence

>Msa0825250

MGSNDLKYEVSQNAYIKLVLHSLKHPTSAVNGVLIGRISSSNEIVEITDAVPLFHSQIPLLPQLEISLILIEEYFSTKGLNIVGYFHANERSDDFELGGVAKNIGDHICRYFPQAAILLLDNKKLEALKKSKSSSAIMQLYVRDASKNWKLVQSDANNRFSLKEPSANVILLDYISSEKWNDIVDFDDHLDDISKDWLNPGLFN*