AlfalfaGEDB | Msa0827230

M. sativa cultivar zhongmu-4 / Msa0827230

KEGG: -
GO: GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827230	XP_039684665.1	88.768	552	60	1	1	550	12	563	0.0	977

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827230	sp\|O64793\|Y1675_ARATH	31.614	601	312	17	24	556	22	591	7.57e-71	246

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827230	A0A396GKK2	88.768	552	60	1	1	550	12	563	0.0	977

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0827230	Msa1189410	0.939153	2.048050e-99	-8.615850e-47
Msa0827230	Msa1243600	0.869201	3.698202e-66	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0827230	MtrunA17_Chr8g0357881	88.768	552	60	1	1	550	12	563	977
Msa0827230	MtrunA17_Chr8g0357801	83.659	563	83	6	1	556	6	566	946
Msa0827230	MtrunA17_Chr8g0358071	73.524	559	137	6	1	550	7	563	840
Msa0827230	MtrunA17_Chr8g0358031	72.906	561	140	6	1	550	7	566	827
Msa0827230	MtrunA17_Chr8g0358081	71.454	557	138	7	1	550	6	548	782
Msa0827230	MtrunA17_Chr8g0357961	68.467	574	145	7	3	550	8	571	780
Msa0827230	MtrunA17_Chr8g0358101	70.557	557	146	6	1	550	6	551	773
Msa0827230	MtrunA17_Chr8g0358121	71.685	558	136	8	1	550	6	549	772
Msa0827230	MtrunA17_Chr8g0357991	68.858	578	141	9	1	550	6	572	761
Msa0827230	MtrunA17_Chr8g0357931	65.244	492	151	8	1	483	6	486	616

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827230	AT1G67520.1	32.852	554	292	14	24	556	22	516	1.60e-74	248
Msa0827230	AT3G16030.3	30.097	618	340	15	27	555	25	639	3.71e-72	243
Msa0827230	AT3G16030.8	30.097	618	340	15	27	555	25	639	3.71e-72	243
Msa0827230	AT3G16030.5	30.097	618	340	15	27	555	25	639	3.71e-72	243
Msa0827230	AT3G16030.4	29.853	613	338	15	27	550	25	634	6.14e-70	239
Msa0827230	AT3G16030.7	29.853	613	338	15	27	550	25	634	7.18e-70	239
Msa0827230	AT3G16030.6	29.853	613	338	15	27	550	25	634	6.06e-69	239
Msa0827230	AT3G16030.1	30.198	606	324	14	27	550	25	613	6.13e-69	238
Msa0827230	AT1G11340.2	30.741	553	292	18	74	552	136	671	3.07e-63	221
Msa0827230	AT1G11340.3	30.741	553	292	18	74	552	136	671	7.66e-63	221

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Find 184 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAATAACAAACCTGT+GGG	0.611114	5_3:+68382633	None:intergenic
AAAATACTATAAAATTACTA+AGG	0.518564	5_3:+68382721	None:intergenic
AAAATTATAAGATGTTCCCA+GGG	0.656440	5_3:-68385585	Msa0827230:CDS
AAAATTATACAATTTACTTT+AGG	0.316531	5_3:+68386472	None:intergenic
AAATGCTGGCCTTCTTGGTA+AGG	0.471963	5_3:+68382977	None:intergenic
AAATTGTGAATTGTATATGT+TGG	0.577998	5_3:+68384162	None:intergenic
AACATCTCGACAAGGAATTG+TGG	0.550299	5_3:-68384392	Msa0827230:CDS
AACCAATGACCAATTATGTC+CGG	0.495276	5_3:+68385828	None:intergenic
AACCATAAGCCACATTGTCT+TGG	0.487692	5_3:+68385383	None:intergenic
AACCGGACATAATTGGTCAT+TGG	0.393190	5_3:-68385830	Msa0827230:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTACTTTATTGTATCACC+TGG	+	chr5_3:68382870-68382889	None:intergenic	30.0%
AACTGCTGAATTTAAGTCAA+TGG	+	chr5_3:68383027-68383046	None:intergenic	30.0%
GGTGAAGAGTAAATGATTAT+TGG	+	chr5_3:68383104-68383123	None:intergenic	30.0%
TTCAACAACTTCACACAAAT+GGG	-	chr5_3:68383177-68383196	Msa0827230:intron	30.0%
TAACACCTAACTTCATTGTT+GGG	+	chr5_3:68383253-68383272	None:intergenic	30.0%
TTAACACCTAACTTCATTGT+TGG	+	chr5_3:68383254-68383273	None:intergenic	30.0%
GTATCAGTACATTATTGACA+AGG	-	chr5_3:68383469-68383488	Msa0827230:intron	30.0%
CAAAATTATAAGATGTTCCC+AGG	-	chr5_3:68383524-68383543	Msa0827230:three_prime_UTR	30.0%
TGTTATGGATATAATCGTGA+TGG	-	chr5_3:68383611-68383630	Msa0827230:three_prime_UTR	30.0%
TCCCATTACGATAAAATTCT+TGG	+	chr5_3:68383802-68383821	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	68382622	68386510	68382622	ID=Msa0827230;Name=Msa0827230
chr5_3	mRNA	68382622	68386510	68382622	ID=Msa0827230-mRNA-1;Parent=Msa0827230;Name=Msa0827230-mRNA-1;_AED=0.05;_eAED=0.05;_QI=155\|1\|1\|1\|0.8\|0.83\|6\|1445\|559
chr5_3	exon	68382622	68383186	68382622	ID=Msa0827230-mRNA-1:exon:21439;Parent=Msa0827230-mRNA-1
chr5_3	exon	68383268	68383418	68383268	ID=Msa0827230-mRNA-1:exon:21438;Parent=Msa0827230-mRNA-1
chr5_3	exon	68383493	68384461	68383493	ID=Msa0827230-mRNA-1:exon:21437;Parent=Msa0827230-mRNA-1
chr5_3	exon	68384603	68384790	68384603	ID=Msa0827230-mRNA-1:exon:21436;Parent=Msa0827230-mRNA-1
chr5_3	exon	68384863	68384970	68384863	ID=Msa0827230-mRNA-1:exon:21435;Parent=Msa0827230-mRNA-1
chr5_3	exon	68385212	68386510	68385212	ID=Msa0827230-mRNA-1:exon:21434;Parent=Msa0827230-mRNA-1
chr5_3	five_prime_UTR	68386356	68386510	68386356	ID=Msa0827230-mRNA-1:five_prime_utr;Parent=Msa0827230-mRNA-1
chr5_3	CDS	68385212	68386355	68385212	ID=Msa0827230-mRNA-1:cds;Parent=Msa0827230-mRNA-1
chr5_3	CDS	68384863	68384970	68384863	ID=Msa0827230-mRNA-1:cds;Parent=Msa0827230-mRNA-1
chr5_3	CDS	68384603	68384790	68384603	ID=Msa0827230-mRNA-1:cds;Parent=Msa0827230-mRNA-1
chr5_3	CDS	68384222	68384461	68384222	ID=Msa0827230-mRNA-1:cds;Parent=Msa0827230-mRNA-1
chr5_3	three_prime_UTR	68383493	68384221	68383493	ID=Msa0827230-mRNA-1:three_prime_utr;Parent=Msa0827230-mRNA-1
chr5_3	three_prime_UTR	68383268	68383418	68383268	ID=Msa0827230-mRNA-1:three_prime_utr;Parent=Msa0827230-mRNA-1
chr5_3	three_prime_UTR	68382622	68383186	68382622	ID=Msa0827230-mRNA-1:three_prime_utr;Parent=Msa0827230-mRNA-1

Gene Sequence

>Msa0827230

ATGAAGAAACTAGTTGCATTGATATGCTTATGGTTGTGGTGGAGTTCTACTTCTATTATTTGTGCTAATGCAACAAATGATAGTTTGAAGCCAGGTGATACAATAAAGTACAAATCCAAGCTATGTTCTAAACAAGGCAAGTATTGTCTATATTTCAATCGAACATTAGATAGCGAGGATGCTCACTTGTTCATTGGTGTTAATGCTGATTATGGAAAAGTAGTGTGGATGCATGATAGAAATCATTCCATTGACTTAAATTCAGCAGTTCTATCATTAGACTACTCTGGTGTGCTCAAAATAGAATCACAAAATAGGAATTTACCAATAATCATTTACTCTTCACCTCAACCCTCCAACAACACTGTCGCCACTATGTTAGACACCGGTAACTTTGTGCTTCAACAACTTCACACAAATGGGACAAAGAGTATATTATGGCAGAGTTTTGATTATCCCACAGCCACTTTGATCCCAACAATGAAGTTAGGTGTTAACCGAAAAACCGGACATAATTGGTCATTGGTTTCATGGTTGGCTCCGTCACTACCAAATTCAGGTGGATTTAGTCTTGAATGGGAACCTAACGAAGGTGAATTGAACATCAAGCAACGCGGCAAAGTGTATTGGAAAAGCGGAAAACGTAGAAGCAATGGTTTATTCGAGAACATTCCAGTGAAAATTCAACGAGTGTATCAGTACATTATTGACAAGGATGAAGACTCTTTCACTTTCGAAATCAAAGATCAAAATTATAAGATGTTCCCAGGGTGGGAACTGTTCTCAACAGGGATGTTAACGAGTAGTGAAGGAGAGATTGCTAATGCAGATGTGTGTTATGGATATAATCGTGATGGAGGGTGCCAAAAATGGGAGGATATACCAACTTGCAGGGAACCTGGTGAGGTGTTTCAAATAAAGAAAGGCAGACCCAAAACCAGGGCAACCGTCCAAGACAATGTGGCTTATGGTTATAGTGACTGTAAGCTCAGTTGTTGGAGAAACTGTGACTGCAATGGGTTCCAAGAATTTTATCGTAATGGGACTGGATGTATATTTTATTCTTCGAATTCGACAAAAGATGTTGATTGGGAGTATCCAAATATCTTTAACGTCATGGTAAAGCCAACGCTAAATCACCATCGTAAAAACATGTGGATATTGATAGGTGCTGCAATAGCAGCTGCTATACTCATATTATGCACACTCCTTTTATGTGTAGCCAAAAGGAAACAAAAATATGCGCGTAAAGATAAGAAAAGTAAAAGAAAGGAAGATAAGAGCAATGATTTAGCGGAATTCTACGATATCAAAGATCTTGAAGATGACTTCAAGGGACATGATATAAAAGTTTTTAACTATACATCAATTTTGGAAGCAACTATGGACTTTTCACCTGAAAACAAGTTAGGACAAGGAGGTTATGGACCTGTTTATAAGGGAATTTTGCCAACAGGGCAAGAAATTGCTGTAAAAAGACTTTCAAAAACATCTCGACAAGGAATTGTGGAATTCAAAAATGAATTGGTACTTATATGTGAACTTCAGCACAGAAATCTTGTACAACTACTTGGTTGTTGCATTCATGAAGAAGAAAGGATTCTAATATATGAATACATGTCAAACAAAAGCTTGGATTTCTATCTATTTGGTGAGAATGTGTTGGACAAATTCATATAG

Protein sequence

>Msa0827230

MKKLVALICLWLWWSSTSIICANATNDSLKPGDTIKYKSKLCSKQGKYCLYFNRTLDSEDAHLFIGVNADYGKVVWMHDRNHSIDLNSAVLSLDYSGVLKIESQNRNLPIIIYSSPQPSNNTVATMLDTGNFVLQQLHTNGTKSILWQSFDYPTATLIPTMKLGVNRKTGHNWSLVSWLAPSLPNSGGFSLEWEPNEGELNIKQRGKVYWKSGKRRSNGLFENIPVKIQRVYQYIIDKDEDSFTFEIKDQNYKMFPGWELFSTGMLTSSEGEIANADVCYGYNRDGGCQKWEDIPTCREPGEVFQIKKGRPKTRATVQDNVAYGYSDCKLSCWRNCDCNGFQEFYRNGTGCIFYSSNSTKDVDWEYPNIFNVMVKPTLNHHRKNMWILIGAAIAAAILILCTLLLCVAKRKQKYARKDKKSKRKEDKSNDLAEFYDIKDLEDDFKGHDIKVFNYTSILEATMDFSPENKLGQGGYGPVYKGILPTGQEIAVKRLSKTSRQGIVEFKNELVLICELQHRNLVQLLGCCIHEEERILIYEYMSNKSLDFYLFGENVLDKFI*