AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0852640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0852640 MtrunA17_Chr5g0429111 94.099 322 3 1 1 306 1 322 0.0 572
Msa0852640 MtrunA17_Chr3g0128091 61.184 304 69 8 28 306 5 284 3.46e-90 270
Msa0852640 MtrunA17_Chr8g0343041 57.277 213 48 3 133 306 81 289 1.73e-52 174
Msa0852640 MtrunA17_Chr8g0386231 34.028 288 139 8 52 305 50 320 3.90e-27 108
Msa0852640 MtrunA17_Chr1g0180631 27.469 324 179 4 38 305 9 332 2.97e-24 100
Msa0852640 MtrunA17_Chr3g0132051 30.292 274 122 7 56 305 37 265 9.44e-21 89.7
Msa0852640 MtrunA17_Chr3g0132051 32.432 185 99 5 31 189 10 194 1.10e-14 72.8
Msa0852640 MtrunA17_Chr4g0028461 25.912 274 160 5 56 305 69 323 7.81e-19 85.1
Msa0852640 MtrunA17_Chr3g0132061 33.125 160 82 3 56 190 37 196 1.42e-18 84.3
Msa0852640 MtrunA17_Chr3g0094841 22.182 275 135 5 50 305 22 236 3.11e-15 73.9
Msa0852640 MtrunA17_Chr8g0337951 24.823 282 159 6 43 306 19 265 1.40e-14 72.4
Msa0852640 MtrunA17_Chr2g0327311 27.647 170 93 4 52 191 64 233 2.50e-14 72.4
Msa0852640 MtrunA17_Chr2g0327311 37.363 91 53 1 32 118 142 232 6.33e-11 62.4
Msa0852640 MtrunA17_Chr2g0290471 30.142 282 126 7 50 305 32 268 2.63e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0852640 AT5G24580.4 58.545 275 76 5 56 306 59 319 4.78e-92 276
Msa0852640 AT5G24580.1 58.545 275 76 5 56 306 59 319 4.78e-92 276
Msa0852640 AT5G24580.2 58.545 275 76 5 56 306 58 318 5.33e-92 276
Msa0852640 AT5G24580.3 57.455 275 76 6 56 306 59 316 7.55e-88 265
Msa0852640 AT5G24580.5 56.098 246 70 5 85 306 1 232 1.86e-72 223
Msa0852640 AT5G50740.5 30.576 278 140 7 56 305 30 282 1.50e-26 106
Msa0852640 AT5G50740.1 30.576 278 140 7 56 305 30 282 1.50e-26 106
Msa0852640 AT5G50740.3 30.576 278 140 7 56 305 37 289 9.14e-26 104
Msa0852640 AT5G63530.2 32.919 161 82 3 56 190 61 221 1.71e-19 87.8
Msa0852640 AT5G63530.1 32.919 161 82 3 56 190 76 236 2.05e-19 87.8
Msa0852640 AT2G36950.1 27.405 343 146 13 56 305 53 385 1.77e-14 73.6
Msa0852640 AT3G02960.1 27.322 183 112 4 29 190 9 191 1.98e-13 69.3
Msa0852640 AT5G03380.1 38.710 93 56 1 31 123 3 94 9.29e-11 62.4

Find 99 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGTAACAATTCTGATTT+TGG 0.113182 5_4:-43835556 None:intergenic
AGCACCACTCTCTTCTTTCT+TGG 0.193164 5_4:-43838089 None:intergenic
ACGCGCGCGGATTTGCTAAT+TGG 0.218649 5_4:+43838546 Msa0852640:three_prime_UTR
TCTTCTTTCTTGTTCTCTTC+TGG 0.221644 5_4:-43837983 None:intergenic
ACCTGAGAATTAACAACTTC+TGG 0.221996 5_4:-43836515 None:intergenic
TCTGGTTCAGGTTCTGGTTT+AGG 0.243629 5_4:-43837902 None:intergenic
TGATACTTCATACACCAAAA+TGG 0.245374 5_4:+43835576 Msa0852640:exon
CTTAACCTATGTCGTAATTT+AGG 0.248364 5_4:+43838697 Msa0852640:three_prime_UTR
AAAGCCAGAAGAAGAGAAAA+AGG 0.250871 5_4:+43836138 Msa0852640:CDS
ATAAAGGAGTTTAAGATATA+TGG 0.250909 5_4:-43835456 None:intergenic
CAAGTATATAGAGAATTAAA+AGG 0.274034 5_4:-43835485 None:intergenic
CTCTTCTTTCTTTGGTTCCT+CGG 0.291585 5_4:-43838017 None:intergenic
ACACATGGTTCTGGTGGTTT+AGG 0.318590 5_4:-43836166 None:intergenic
TCTTGCTTCTCCTCAACCTT+TGG 0.319046 5_4:-43836049 None:intergenic
TTTCATGGATCTGGATTCGC+TGG 0.341121 5_4:+43838511 Msa0852640:three_prime_UTR
TATGAAATGCTGCATGCATT+AGG 0.356350 5_4:-43838229 None:intergenic
CTTTCTCTCTCTTTCCCACT+TGG 0.370964 5_4:+43835232 Msa0852640:five_prime_UTR
AACAAAGTATACCTCTCATT+TGG 0.383717 5_4:-43837059 None:intergenic
TTAGCTTCTTCTTCTGCTGC+AGG 0.384645 5_4:-43837959 None:intergenic
AAAGAAGAAGAAAGCAAAGA+AGG 0.390606 5_4:+43837929 Msa0852640:CDS
AATTAAAAGGGATATTATAA+AGG 0.396378 5_4:-43835472 None:intergenic
AGGAAAAGAAGAGGAAAAGA+AGG 0.400221 5_4:+43836099 Msa0852640:CDS
GGGACAGGTTCTCCTTCTGC+TGG 0.400570 5_4:-43836494 None:intergenic
CCCAGAAGTTGTTAATTCTC+AGG 0.407079 5_4:+43836514 Msa0852640:CDS
TAAAAGGGTTTGAAGATTGA+AGG 0.408404 5_4:-43835313 None:intergenic
TCTTTCTTTGGTTCCTCGGT+TGG 0.414826 5_4:-43838013 None:intergenic
CCTGAGAATTAACAACTTCT+GGG 0.425360 5_4:-43836514 None:intergenic
TAGTGGCTTTGCATGTGATC+AGG 0.427973 5_4:+43835386 Msa0852640:five_prime_UTR
AATGGGTGAGGAAGCAAAAC+AGG 0.430083 5_4:+43835594 Msa0852640:exon
AAGATATATGGCTGCCTGGC+TGG 0.431834 5_4:-43835444 None:intergenic
GATACTTCATACACCAAAAT+GGG 0.437255 5_4:+43835577 Msa0852640:exon
GGGTGAGAATAAAGAAGAAA+AGG 0.462764 5_4:+43838042 Msa0852640:CDS
AGAGGAGAAGAAAGAAGAAA+AGG 0.465810 5_4:+43836069 Msa0852640:CDS
TCTCCTTCTGCTGGAGGCAA+TGG 0.469954 5_4:-43836485 None:intergenic
GGCAGAAGGAGGAAAAGAAG+AGG 0.483712 5_4:+43836090 Msa0852640:CDS
GGTGAGAATAAAGAAGAAAA+GGG 0.485122 5_4:+43838043 Msa0852640:CDS
TTATCAGCTTTATAGACAAA+AGG 0.486698 5_4:-43835270 None:intergenic
TTTGTGGATTTGCATTGTGT+TGG 0.488520 5_4:+43836193 Msa0852640:CDS
AAAGAAGAAAAGGCAGAAGG+AGG 0.491619 5_4:+43836079 Msa0852640:CDS
GCAAAGCCACTAACTGCAAA+AGG 0.494035 5_4:-43835375 None:intergenic
GTGAGAATAAAGAAGAAAAG+GGG 0.501789 5_4:+43838044 Msa0852640:CDS
AGTTACCATAAAAGGTATAG+TGG 0.502480 5_4:+43836400 Msa0852640:CDS
AAGTATATAGAGAATTAAAA+GGG 0.507048 5_4:-43835484 None:intergenic
AAAAGGGTTTGAAGATTGAA+GGG 0.507620 5_4:-43835312 None:intergenic
AAAAGAAGAGGAAAAGAAGG+AGG 0.513975 5_4:+43836102 Msa0852640:CDS
GTACGTTCAATCACATAAAG+TGG 0.523350 5_4:-43838175 None:intergenic
GAATTAACAACTTCTGGGAC+AGG 0.524343 5_4:-43836509 None:intergenic
GCATGTGATCAGGAATCACT+TGG 0.527910 5_4:+43835396 Msa0852640:five_prime_UTR
TATACTTAAGCATACAAAAG+AGG 0.530265 5_4:-43835521 None:intergenic
ATTGTATTGCATATAGTTTG+AGG 0.532202 5_4:-43836425 None:intergenic
TTCATGGATCTGGATTCGCT+GGG 0.532741 5_4:+43838512 Msa0852640:three_prime_UTR
AAGAAAGAAGAAAAGGCAGA+AGG 0.538785 5_4:+43836076 Msa0852640:CDS
GCAGTAACAGAGTTTAGCAC+AGG 0.539831 5_4:+43837794 Msa0852640:CDS
ACAGGTTCTCCTTCTGCTGG+AGG 0.543046 5_4:-43836491 None:intergenic
AAAGAAGGAGGAAAGCAAAG+AGG 0.546725 5_4:+43836114 Msa0852640:CDS
GAGGAACCAAAGAAAGAAGA+GGG 0.549886 5_4:+43838019 Msa0852640:CDS
ACAGGGAAAGTAACTGTGAC+AGG 0.550976 5_4:+43837812 Msa0852640:CDS
TTTGTTGGTGGAATATGGAG+AGG 0.551259 5_4:+43838306 Msa0852640:three_prime_UTR
TCTCCATTGCCTCCAGCAGA+AGG 0.554781 5_4:+43836482 Msa0852640:CDS
TCACTGAATAGTTGAGGAGG+TGG 0.557788 5_4:-43838199 None:intergenic
GTTTAAGATATATGGCTGCC+TGG 0.568992 5_4:-43835448 None:intergenic
AATCTTAATCACTACCAGCC+AGG 0.570376 5_4:+43835430 Msa0852640:five_prime_UTR
CGAGGAACCAAAGAAAGAAG+AGG 0.574021 5_4:+43838018 Msa0852640:CDS
GTGGTTAATATTGATGAAGA+AGG 0.577972 5_4:+43838118 Msa0852640:CDS
ACTGACCTAAATTACGACAT+AGG 0.583759 5_4:-43838702 None:intergenic
AACTGCAAAAGGAATCACTA+AGG 0.586211 5_4:-43835364 None:intergenic
GTGGGAAAGAGAGAGAAAGT+AGG 0.593575 5_4:-43835228 None:intergenic
AGTAACTGTGACAGGAACAA+TGG 0.597289 5_4:+43837820 Msa0852640:CDS
AATGGATGCAAACAAGCTAG+TGG 0.597513 5_4:+43837838 Msa0852640:CDS
TTCATACACCAAAATGGGTG+AGG 0.598463 5_4:+43835582 Msa0852640:exon
ATAGGAACAACCAAAGGTTG+AGG 0.604946 5_4:+43836039 Msa0852640:intron
ACAAAAGGAGATTAATAGTG+AGG 0.608483 5_4:-43835255 None:intergenic
TCATCACTGAATAGTTGAGG+AGG 0.609687 5_4:-43838202 None:intergenic
ATCAAAATCGATCATCGAGA+CGG 0.609950 5_4:-43838606 None:intergenic
AGATTAATAGTGAGGCCAAG+TGG 0.610996 5_4:-43835247 None:intergenic
GATTAATAGTGAGGCCAAGT+GGG 0.614938 5_4:-43835246 None:intergenic
AAAAGTACACATGGTTCTGG+TGG 0.618037 5_4:-43836172 None:intergenic
AGGAACCAAAGAAAGAAGAG+GGG 0.622785 5_4:+43838020 Msa0852640:CDS
GATCCATGAAAAGCAACTTG+AGG 0.632435 5_4:-43838499 None:intergenic
GGAACCAAAGAAAGAAGAGG+GGG 0.638940 5_4:+43838021 Msa0852640:CDS
AATTTAGGTCAGTAGTGACA+AGG 0.639913 5_4:+43838712 Msa0852640:three_prime_UTR
TGAGAATAAAGAAGAAAAGG+GGG 0.645103 5_4:+43838045 Msa0852640:CDS
TTGAGTATAGGAACAACCAA+AGG 0.645154 5_4:+43836033 Msa0852640:intron
ATTTAGGTCAGTAGTGACAA+GGG 0.645898 5_4:+43838713 Msa0852640:three_prime_UTR
TGAGGGAGTAGTTATTGACA+TGG 0.646592 5_4:+43836367 Msa0852640:CDS
AAAGGTTGAGGAGAAGCAAG+AGG 0.647168 5_4:+43836051 Msa0852640:CDS
AGAAAGATACTCCAAATGAG+AGG 0.650465 5_4:+43837048 Msa0852640:CDS
CAGTAACAGAGTTTAGCACA+GGG 0.652827 5_4:+43837795 Msa0852640:CDS
AGATCTATAATGAAAATGAG+AGG 0.657418 5_4:+43836235 Msa0852640:CDS
GAAACCAAGAAAGAAGAGAG+TGG 0.657886 5_4:+43838085 Msa0852640:CDS
CGTTCAATCACATAAAGTGG+AGG 0.659105 5_4:-43838172 None:intergenic
AGAGAGTGGTGCTACTGCTG+TGG 0.672236 5_4:+43838099 Msa0852640:CDS
TCATGGATCTGGATTCGCTG+GGG 0.674384 5_4:+43838513 Msa0852640:three_prime_UTR
TTTAGGTCAGTAGTGACAAG+GGG 0.683844 5_4:+43838714 Msa0852640:three_prime_UTR
GAGAATAAAGAAGAAAAGGG+GGG 0.694515 5_4:+43838046 Msa0852640:CDS
GAACCAAAGAAAGAAGAGGG+GGG 0.694939 5_4:+43838022 Msa0852640:CDS
AGGGATGAAGCAGTGATGTG+AGG 0.703564 5_4:-43835293 None:intergenic
GGGTAATGATACTACGCGCG+CGG 0.716245 5_4:+43838533 Msa0852640:three_prime_UTR
AGAAGAAGAGAAACCAACCG+AGG 0.717508 5_4:+43838000 Msa0852640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTATAAACTGAAATAA+AGG - chr5_4:43836902-43836921 None:intergenic 10.0%
!! TTAATGATAAAATATTACAA+AGG + chr5_4:43837255-43837274 Msa0852640:intron 10.0%
!! TAATGATAAAATATTACAAA+GGG + chr5_4:43837256-43837275 Msa0852640:intron 10.0%
!! TTAATTAATGCAAATAAATA+TGG - chr5_4:43837736-43837755 None:intergenic 10.0%
!! TAATAATATACATAAAAAAG+AGG - chr5_4:43838398-43838417 None:intergenic 10.0%
!! AATTAAAAGGGATATTATAA+AGG - chr5_4:43835475-43835494 None:intergenic 15.0%
!! AAGTATATAGAGAATTAAAA+GGG - chr5_4:43835487-43835506 None:intergenic 15.0%
!!! TAAATCATGATTTTAAATTG+CGG + chr5_4:43835749-43835768 Msa0852640:intron 15.0%
!! TTAAGAGATCATATAAAAAA+AGG + chr5_4:43836563-43836582 Msa0852640:intron 15.0%
!! TAAGAGATCATATAAAAAAA+GGG + chr5_4:43836564-43836583 Msa0852640:intron 15.0%
!!! ATTTTTTTTTCTTTTCATTG+AGG - chr5_4:43837236-43837255 None:intergenic 15.0%
!! AATGATAAAATATTACAAAG+GGG + chr5_4:43837257-43837276 Msa0852640:intron 15.0%
!!! ATGATTCATAAAATTTTGTT+CGG - chr5_4:43837625-43837644 None:intergenic 15.0%
!!! AGAACAATATGAATTTTTTT+TGG + chr5_4:43838280-43838299 Msa0852640:three_prime_UTR 15.0%
!!! TTGTACAACATTTTTTTTTA+TGG - chr5_4:43838458-43838477 None:intergenic 15.0%
!!! ATAAAGGAGTTTAAGATATA+TGG - chr5_4:43835459-43835478 None:intergenic 20.0%
!! CAAGTATATAGAGAATTAAA+AGG - chr5_4:43835488-43835507 None:intergenic 20.0%
!! AAATCTTTGTACCATAAAAA+AGG + chr5_4:43835781-43835800 Msa0852640:intron 20.0%
!!! ATTCTTTTTTGATTGAGTAT+AGG + chr5_4:43836021-43836040 Msa0852640:intron 20.0%
!! AAAAATGAAGTTACCATAAA+AGG + chr5_4:43836392-43836411 Msa0852640:CDS 20.0%
!! TGATCTCTTAAAAATAAAAG+AGG - chr5_4:43836554-43836573 None:intergenic 20.0%
!!! AGGTTATCATAATCTAATTT+TGG + chr5_4:43837505-43837524 Msa0852640:intron 20.0%
!!! AAACTTGTATTGATTAATGT+AGG + chr5_4:43837761-43837780 Msa0852640:intron 20.0%
!!! AACTATTTTTGCTTGTTTTT+TGG - chr5_4:43837882-43837901 None:intergenic 20.0%
!!! AATTTTTTTTGGAGTTTTGT+TGG + chr5_4:43838291-43838310 Msa0852640:three_prime_UTR 20.0%
!! TTATCAGCTTTATAGACAAA+AGG - chr5_4:43835273-43835292 None:intergenic 25.0%
! TATACTTAAGCATACAAAAG+AGG - chr5_4:43835524-43835543 None:intergenic 25.0%
!! TCAAGTAACAATTCTGATTT+TGG - chr5_4:43835559-43835578 None:intergenic 25.0%
! CTATTATAACTCAACTAAGA+GGG - chr5_4:43835655-43835674 None:intergenic 25.0%
! TCTATTATAACTCAACTAAG+AGG - chr5_4:43835656-43835675 None:intergenic 25.0%
! AGATCTATAATGAAAATGAG+AGG + chr5_4:43836235-43836254 Msa0852640:CDS 25.0%
!! GTGTTTATCTTTCTTTTATC+AGG + chr5_4:43836341-43836360 Msa0852640:intron 25.0%
! ATTGTATTGCATATAGTTTG+AGG - chr5_4:43836428-43836447 None:intergenic 25.0%
! ATCGACAAACATATGTATTA+GGG - chr5_4:43836663-43836682 None:intergenic 25.0%
!! AATTTTACATTTGTCAGTGT+CGG + chr5_4:43836714-43836733 Msa0852640:intron 25.0%
! AATAAGCAGAAAAGAACTTA+TGG + chr5_4:43836838-43836857 Msa0852640:intron 25.0%
!! TTTTACTTCTTATCGAGAAA+AGG + chr5_4:43837114-43837133 Msa0852640:intron 25.0%
! TGATACAAAACATGAAAAGA+TGG - chr5_4:43837326-43837345 None:intergenic 25.0%
!! ATTCTTTTGAACAAATAGGT+TGG + chr5_4:43837395-43837414 Msa0852640:intron 25.0%
!!! ACTATTTCTCTTACTTTTTG+AGG - chr5_4:43837471-43837490 None:intergenic 25.0%
! AAAAAGTAAGAGAAATAGTG+AGG + chr5_4:43837472-43837491 Msa0852640:intron 25.0%
! AAAAGTAAGAGAAATAGTGA+GGG + chr5_4:43837473-43837492 Msa0852640:intron 25.0%
ACAAAAGGAGATTAATAGTG+AGG - chr5_4:43835258-43835277 None:intergenic 30.0%
AAAAGGGTTTGAAGATTGAA+GGG - chr5_4:43835315-43835334 None:intergenic 30.0%
TAAAAGGGTTTGAAGATTGA+AGG - chr5_4:43835316-43835335 None:intergenic 30.0%
TGATACTTCATACACCAAAA+TGG + chr5_4:43835576-43835595 Msa0852640:exon 30.0%
GATACTTCATACACCAAAAT+GGG + chr5_4:43835577-43835596 Msa0852640:exon 30.0%
GCAGACAACAATTTAAACAA+TGG + chr5_4:43835884-43835903 Msa0852640:intron 30.0%
! CCATTTTATAACTCTTGTCA+AGG - chr5_4:43835919-43835938 None:intergenic 30.0%
CCTTGACAAGAGTTATAAAA+TGG + chr5_4:43835916-43835935 Msa0852640:intron 30.0%
ACATTCGTGAGTTTGTAAAA+TGG + chr5_4:43835959-43835978 Msa0852640:intron 30.0%
!! AGTTTACATTAGTTCTGTCA+TGG + chr5_4:43835991-43836010 Msa0852640:intron 30.0%
! AAATCCACAAAAAGTACACA+TGG - chr5_4:43836184-43836203 None:intergenic 30.0%
! TTTCTTTTATCAGGAGTTGA+GGG + chr5_4:43836350-43836369 Msa0852640:intron 30.0%
! AGTTACCATAAAAGGTATAG+TGG + chr5_4:43836400-43836419 Msa0852640:CDS 30.0%
CATATAAAAAAAGGGAAAGC+TGG + chr5_4:43836572-43836591 Msa0852640:intron 30.0%
GATCGACAAACATATGTATT+AGG - chr5_4:43836664-43836683 None:intergenic 30.0%
!! CTTATTTTCATACGCTTTTG+AGG - chr5_4:43836824-43836843 None:intergenic 30.0%
AACAAAGTATACCTCTCATT+TGG - chr5_4:43837062-43837081 None:intergenic 30.0%
AAAGGACATGTAGAGATAAA+AGG + chr5_4:43837132-43837151 Msa0852640:intron 30.0%
AAGGACATGTAGAGATAAAA+GGG + chr5_4:43837133-43837152 Msa0852640:intron 30.0%
! TTGATGGTTCATCCTTTAAT+TGG - chr5_4:43837284-43837303 None:intergenic 30.0%
AAAAGAATGCCACAATAAGA+GGG - chr5_4:43837383-43837402 None:intergenic 30.0%
! TGGCATTCTTTTGAACAAAT+AGG + chr5_4:43837391-43837410 Msa0852640:intron 30.0%
AAAGTAAGAGAAATAGTGAG+GGG + chr5_4:43837474-43837493 Msa0852640:intron 30.0%
TGTCTTAAGCATTTGATTCA+TGG - chr5_4:43837685-43837704 None:intergenic 30.0%
! CTTTCTTCTTCTTTCTTTTC+TGG - chr5_4:43837923-43837942 None:intergenic 30.0%
AAAGAAGAAGAAAGCAAAGA+AGG + chr5_4:43837929-43837948 Msa0852640:CDS 30.0%
GGTGAGAATAAAGAAGAAAA+GGG + chr5_4:43838043-43838062 Msa0852640:CDS 30.0%
GTGAGAATAAAGAAGAAAAG+GGG + chr5_4:43838044-43838063 Msa0852640:CDS 30.0%
TGAGAATAAAGAAGAAAAGG+GGG + chr5_4:43838045-43838064 Msa0852640:CDS 30.0%
GTGGTTAATATTGATGAAGA+AGG + chr5_4:43838118-43838137 Msa0852640:CDS 30.0%
! TTTTCATCACTGAATAGTTG+AGG - chr5_4:43838208-43838227 None:intergenic 30.0%
!!! TTTTTTTGGAGTTTTGTTGG+TGG + chr5_4:43838294-43838313 Msa0852640:three_prime_UTR 30.0%
GAGGAAAAAATGTGTCTAAA+TGG + chr5_4:43838325-43838344 Msa0852640:three_prime_UTR 30.0%
CTTAACCTATGTCGTAATTT+AGG + chr5_4:43838697-43838716 Msa0852640:three_prime_UTR 30.0%
AACTGCAAAAGGAATCACTA+AGG - chr5_4:43835367-43835386 None:intergenic 35.0%
TTGAGTATAGGAACAACCAA+AGG + chr5_4:43836033-43836052 Msa0852640:intron 35.0%
AGAGGAGAAGAAAGAAGAAA+AGG + chr5_4:43836069-43836088 Msa0852640:CDS 35.0%
AAGAAAGAAGAAAAGGCAGA+AGG + chr5_4:43836076-43836095 Msa0852640:CDS 35.0%
AGGAAAAGAAGAGGAAAAGA+AGG + chr5_4:43836099-43836118 Msa0852640:CDS 35.0%
AAAAGAAGAGGAAAAGAAGG+AGG + chr5_4:43836102-43836121 Msa0852640:CDS 35.0%
AAAGCCAGAAGAAGAGAAAA+AGG + chr5_4:43836138-43836157 Msa0852640:CDS 35.0%
! TCTTCCTTTTTCTCTTCTTC+TGG - chr5_4:43836145-43836164 None:intergenic 35.0%
! ACAAAAAGTACACATGGTTC+TGG - chr5_4:43836178-43836197 None:intergenic 35.0%
!! AGAACCATGTGTACTTTTTG+TGG + chr5_4:43836177-43836196 Msa0852640:CDS 35.0%
TTTGTGGATTTGCATTGTGT+TGG + chr5_4:43836193-43836212 Msa0852640:CDS 35.0%
! CTTTCTTTTATCAGGAGTTG+AGG + chr5_4:43836349-43836368 Msa0852640:intron 35.0%
!!! TGCTCTTCTCTTTGTTTTCT+TGG - chr5_4:43836453-43836472 None:intergenic 35.0%
CCTGAGAATTAACAACTTCT+GGG - chr5_4:43836517-43836536 None:intergenic 35.0%
ACCTGAGAATTAACAACTTC+TGG - chr5_4:43836518-43836537 None:intergenic 35.0%
! ATATGTATTAGGGTCTTGTC+CGG - chr5_4:43836653-43836672 None:intergenic 35.0%
AACATGTCCTTAATCCACAA+TGG + chr5_4:43836937-43836956 Msa0852640:intron 35.0%
ACATGTCCTTAATCCACAAT+GGG + chr5_4:43836938-43836957 Msa0852640:intron 35.0%
! CAAATTCCCATTGTGGATTA+AGG - chr5_4:43836947-43836966 None:intergenic 35.0%
AATCCACAATGGGAATTTGT+TGG + chr5_4:43836948-43836967 Msa0852640:intron 35.0%
AGAAAGATACTCCAAATGAG+AGG + chr5_4:43837048-43837067 Msa0852640:CDS 35.0%
AGGACATGTAGAGATAAAAG+GGG + chr5_4:43837134-43837153 Msa0852640:intron 35.0%
TTACAAAGGGGTCCAATTAA+AGG + chr5_4:43837269-43837288 Msa0852640:intron 35.0%
TGAACCATCAATCCTGAATT+TGG + chr5_4:43837293-43837312 Msa0852640:intron 35.0%
GAACCATCAATCCTGAATTT+GGG + chr5_4:43837294-43837313 Msa0852640:intron 35.0%
! ACCATCAATTAATGCTGACT+TGG - chr5_4:43837357-43837376 None:intergenic 35.0%
AAGTCAGCATTAATTGATGG+TGG + chr5_4:43837356-43837375 Msa0852640:intron 35.0%
CAAAAGAATGCCACAATAAG+AGG - chr5_4:43837384-43837403 None:intergenic 35.0%
AAGTAAGAGAAATAGTGAGG+GGG + chr5_4:43837475-43837494 Msa0852640:intron 35.0%
!! ATTTTGTTCGGTGAATGACA+TGG - chr5_4:43837613-43837632 None:intergenic 35.0%
GCATTTGATTCATGGTCAAA+CGG - chr5_4:43837677-43837696 None:intergenic 35.0%
!!! TCTTCTTTCTTTTCTGGTTC+AGG - chr5_4:43837917-43837936 None:intergenic 35.0%
TCTTCTTTCTTGTTCTCTTC+TGG - chr5_4:43837986-43838005 None:intergenic 35.0%
GGGTGAGAATAAAGAAGAAA+AGG + chr5_4:43838042-43838061 Msa0852640:CDS 35.0%
GAGAATAAAGAAGAAAAGGG+GGG + chr5_4:43838046-43838065 Msa0852640:CDS 35.0%
GTACGTTCAATCACATAAAG+TGG - chr5_4:43838178-43838197 None:intergenic 35.0%
TATGAAATGCTGCATGCATT+AGG - chr5_4:43838232-43838251 None:intergenic 35.0%
!!! TATCCTCAAGTTGCTTTTCA+TGG + chr5_4:43838496-43838515 Msa0852640:three_prime_UTR 35.0%
ATCAAAATCGATCATCGAGA+CGG - chr5_4:43838609-43838628 None:intergenic 35.0%
!!! GATGATCGATTTTGATTGAG+CGG + chr5_4:43838613-43838632 Msa0852640:three_prime_UTR 35.0%
ACTGACCTAAATTACGACAT+AGG - chr5_4:43838705-43838724 None:intergenic 35.0%
GATTAATAGTGAGGCCAAGT+GGG - chr5_4:43835249-43835268 None:intergenic 40.0%
AGATTAATAGTGAGGCCAAG+TGG - chr5_4:43835250-43835269 None:intergenic 40.0%
! GTGATTCCTTTTGCAGTTAG+TGG + chr5_4:43835369-43835388 Msa0852640:five_prime_UTR 40.0%
AATCTTAATCACTACCAGCC+AGG + chr5_4:43835430-43835449 Msa0852640:five_prime_UTR 40.0%
! GTTTAAGATATATGGCTGCC+TGG - chr5_4:43835451-43835470 None:intergenic 40.0%
TTCATACACCAAAATGGGTG+AGG + chr5_4:43835582-43835601 Msa0852640:exon 40.0%
! TTTTGCTTCCTCACCCATTT+TGG - chr5_4:43835593-43835612 None:intergenic 40.0%
ATAGGAACAACCAAAGGTTG+AGG + chr5_4:43836039-43836058 Msa0852640:intron 40.0%
AAAGAAGAAAAGGCAGAAGG+AGG + chr5_4:43836079-43836098 Msa0852640:CDS 40.0%
AAAGAAGGAGGAAAGCAAAG+AGG + chr5_4:43836114-43836133 Msa0852640:CDS 40.0%
!! AAAAGTACACATGGTTCTGG+TGG - chr5_4:43836175-43836194 None:intergenic 40.0%
CATGATGCATGCATGACATT+TGG - chr5_4:43836304-43836323 None:intergenic 40.0%
TGAGGGAGTAGTTATTGACA+TGG + chr5_4:43836367-43836386 Msa0852640:CDS 40.0%
! AGGCTCCACTATACCTTTTA+TGG - chr5_4:43836408-43836427 None:intergenic 40.0%
!! GAATTAACAACTTCTGGGAC+AGG - chr5_4:43836512-43836531 None:intergenic 40.0%
CCCAGAAGTTGTTAATTCTC+AGG + chr5_4:43836514-43836533 Msa0852640:CDS 40.0%
ACACCAACAAATTCCCATTG+TGG - chr5_4:43836954-43836973 None:intergenic 40.0%
!! TGGGAATTTGTTGGTGTTTC+AGG + chr5_4:43836957-43836976 Msa0852640:intron 40.0%
!! TTGTTGGTGTTTCAGGTTAG+TGG + chr5_4:43836964-43836983 Msa0852640:intron 40.0%
ACATTAAGTTCCACTGTCTC+TGG - chr5_4:43836991-43837010 None:intergenic 40.0%
TAATGTGAACATGCACTGTG+AGG + chr5_4:43837005-43837024 Msa0852640:intron 40.0%
TTGCCCAAATTCAGGATTGA+TGG - chr5_4:43837300-43837319 None:intergenic 40.0%
GCCAAGTCAGCATTAATTGA+TGG + chr5_4:43837353-43837372 Msa0852640:intron 40.0%
!! AGGTTGGTGATTGTGTAATG+TGG + chr5_4:43837411-43837430 Msa0852640:intron 40.0%
!! GGTTGGTGATTGTGTAATGT+GGG + chr5_4:43837412-43837431 Msa0852640:intron 40.0%
ACAATCTCATGCAGAGAACA+TGG - chr5_4:43837447-43837466 None:intergenic 40.0%
CAGTAACAGAGTTTAGCACA+GGG + chr5_4:43837795-43837814 Msa0852640:CDS 40.0%
AGTAACTGTGACAGGAACAA+TGG + chr5_4:43837820-43837839 Msa0852640:CDS 40.0%
AATGGATGCAAACAAGCTAG+TGG + chr5_4:43837838-43837857 Msa0852640:CDS 40.0%
!!! TTCTTTTCTGGTTCAGGTTC+TGG - chr5_4:43837911-43837930 None:intergenic 40.0%
! CTCTTCTTTCTTTGGTTCCT+CGG - chr5_4:43838020-43838039 None:intergenic 40.0%
GAGGAACCAAAGAAAGAAGA+GGG + chr5_4:43838019-43838038 Msa0852640:CDS 40.0%
AGGAACCAAAGAAAGAAGAG+GGG + chr5_4:43838020-43838039 Msa0852640:CDS 40.0%
GAAACCAAGAAAGAAGAGAG+TGG + chr5_4:43838085-43838104 Msa0852640:CDS 40.0%
CGTTCAATCACATAAAGTGG+AGG - chr5_4:43838175-43838194 None:intergenic 40.0%
TCATCACTGAATAGTTGAGG+AGG - chr5_4:43838205-43838224 None:intergenic 40.0%
!!! GGAGTTTTGTTGGTGGAATA+TGG + chr5_4:43838301-43838320 Msa0852640:three_prime_UTR 40.0%
! TTTGTTGGTGGAATATGGAG+AGG + chr5_4:43838306-43838325 Msa0852640:three_prime_UTR 40.0%
GATCCATGAAAAGCAACTTG+AGG - chr5_4:43838502-43838521 None:intergenic 40.0%
!!! CAAGTTGCTTTTCATGGATC+TGG + chr5_4:43838502-43838521 Msa0852640:three_prime_UTR 40.0%
GTGGGAAAGAGAGAGAAAGT+AGG - chr5_4:43835231-43835250 None:intergenic 45.0%
CTTTCTCTCTCTTTCCCACT+TGG + chr5_4:43835232-43835251 Msa0852640:five_prime_UTR 45.0%
GCAAAGCCACTAACTGCAAA+AGG - chr5_4:43835378-43835397 None:intergenic 45.0%
! TAGTGGCTTTGCATGTGATC+AGG + chr5_4:43835386-43835405 Msa0852640:five_prime_UTR 45.0%
GCATGTGATCAGGAATCACT+TGG + chr5_4:43835396-43835415 Msa0852640:five_prime_UTR 45.0%
AATGGGTGAGGAAGCAAAAC+AGG + chr5_4:43835594-43835613 Msa0852640:exon 45.0%
GTCTACATACATCATTCGCG+AGG + chr5_4:43835676-43835695 Msa0852640:intron 45.0%
CATAAAAAAGGCCGCTGATG+CGG + chr5_4:43835793-43835812 Msa0852640:intron 45.0%
GTTGTCTGCAATCGCAATTG+CGG - chr5_4:43835873-43835892 None:intergenic 45.0%
TCTTGCTTCTCCTCAACCTT+TGG - chr5_4:43836052-43836071 None:intergenic 45.0%
AAAGGTTGAGGAGAAGCAAG+AGG + chr5_4:43836051-43836070 Msa0852640:CDS 45.0%
!! ACACATGGTTCTGGTGGTTT+AGG - chr5_4:43836169-43836188 None:intergenic 45.0%
GATGGACATTGCCCAAATTC+AGG - chr5_4:43837308-43837327 None:intergenic 45.0%
ATGCAGAGAACATGGACCAT+GGG - chr5_4:43837439-43837458 None:intergenic 45.0%
TAAGAGAAATAGTGAGGGGG+AGG + chr5_4:43837478-43837497 Msa0852640:intron 45.0%
AAGAGAAATAGTGAGGGGGA+GGG + chr5_4:43837479-43837498 Msa0852640:intron 45.0%
CCATGGTTAGGTGAATGACA+TGG - chr5_4:43837648-43837667 None:intergenic 45.0%
CCATGTCATTCACCTAACCA+TGG + chr5_4:43837645-43837664 Msa0852640:intron 45.0%
AAACGGACATGACCATGGTT+AGG - chr5_4:43837660-43837679 None:intergenic 45.0%
GCAGTAACAGAGTTTAGCAC+AGG + chr5_4:43837794-43837813 Msa0852640:CDS 45.0%
ACAGGGAAAGTAACTGTGAC+AGG + chr5_4:43837812-43837831 Msa0852640:CDS 45.0%
!! TCTGGTTCAGGTTCTGGTTT+AGG - chr5_4:43837905-43837924 None:intergenic 45.0%
TTAGCTTCTTCTTCTGCTGC+AGG - chr5_4:43837962-43837981 None:intergenic 45.0%
AGAAGAAGAGAAACCAACCG+AGG + chr5_4:43838000-43838019 Msa0852640:CDS 45.0%
! TCTTTCTTTGGTTCCTCGGT+TGG - chr5_4:43838016-43838035 None:intergenic 45.0%
CGAGGAACCAAAGAAAGAAG+AGG + chr5_4:43838018-43838037 Msa0852640:CDS 45.0%
GGAACCAAAGAAAGAAGAGG+GGG + chr5_4:43838021-43838040 Msa0852640:CDS 45.0%
GAACCAAAGAAAGAAGAGGG+GGG + chr5_4:43838022-43838041 Msa0852640:CDS 45.0%
AGCACCACTCTCTTCTTTCT+TGG - chr5_4:43838092-43838111 None:intergenic 45.0%
TCACTGAATAGTTGAGGAGG+TGG - chr5_4:43838202-43838221 None:intergenic 45.0%
TTTCATGGATCTGGATTCGC+TGG + chr5_4:43838511-43838530 Msa0852640:three_prime_UTR 45.0%
TTCATGGATCTGGATTCGCT+GGG + chr5_4:43838512-43838531 Msa0852640:three_prime_UTR 45.0%
!!! TTTAGAAATATATTTTAAAA+GGG - chr5_4:43835331-43835350 None:intergenic 5.0%
!!! TTTTAGAAATATATTTTAAA+AGG - chr5_4:43835332-43835351 None:intergenic 5.0%
AGGGATGAAGCAGTGATGTG+AGG - chr5_4:43835296-43835315 None:intergenic 50.0%
AAGATATATGGCTGCCTGGC+TGG - chr5_4:43835447-43835466 None:intergenic 50.0%
!!! CGCATCAGCGGCCTTTTTTA+TGG - chr5_4:43835795-43835814 None:intergenic 50.0%
CAACCATTAGACCGCATCAG+CGG - chr5_4:43835807-43835826 None:intergenic 50.0%
GGCAGAAGGAGGAAAAGAAG+AGG + chr5_4:43836090-43836109 Msa0852640:CDS 50.0%
!! GATGGTGGACCCTCTTATTG+TGG + chr5_4:43837371-43837390 Msa0852640:intron 50.0%
ATTGTGTAATGTGGGCCCCA+TGG + chr5_4:43837420-43837439 Msa0852640:intron 50.0%
TGCAGAGAACATGGACCATG+GGG - chr5_4:43837438-43837457 None:intergenic 50.0%
CATGCAGAGAACATGGACCA+TGG - chr5_4:43837440-43837459 None:intergenic 50.0%
AATAGTGAGGGGGAGGGAAA+AGG + chr5_4:43837485-43837504 Msa0852640:intron 50.0%
TGGTCAAACGGACATGACCA+TGG - chr5_4:43837665-43837684 None:intergenic 50.0%
TCACCCCCCTCTTCTTTCTT+TGG - chr5_4:43838028-43838047 None:intergenic 50.0%
TCATGGATCTGGATTCGCTG+GGG + chr5_4:43838513-43838532 Msa0852640:three_prime_UTR 50.0%
TCTCCATTGCCTCCAGCAGA+AGG + chr5_4:43836482-43836501 Msa0852640:CDS 55.0%
TCTCCTTCTGCTGGAGGCAA+TGG - chr5_4:43836488-43836507 None:intergenic 55.0%
ACAGGTTCTCCTTCTGCTGG+AGG - chr5_4:43836494-43836513 None:intergenic 55.0%
!! TGAAGCACCGACGCAGATAC+CGG + chr5_4:43836631-43836650 Msa0852640:intron 55.0%
GTCTTGTCCGGTATCTGCGT+CGG - chr5_4:43836641-43836660 None:intergenic 55.0%
! GGTTAGTGGACCAGAGACAG+TGG + chr5_4:43836978-43836997 Msa0852640:intron 55.0%
!! AGAGAGTGGTGCTACTGCTG+TGG + chr5_4:43838099-43838118 Msa0852640:CDS 55.0%
GGGTAATGATACTACGCGCG+CGG + chr5_4:43838533-43838552 Msa0852640:three_prime_UTR 55.0%
!! ACGCGCGCGGATTTGCTAAT+TGG + chr5_4:43838546-43838565 Msa0852640:three_prime_UTR 55.0%
AGGCCGCTGATGCGGTCTAA+TGG + chr5_4:43835801-43835820 Msa0852640:intron 60.0%
GGGACAGGTTCTCCTTCTGC+TGG - chr5_4:43836497-43836516 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 43835179 43838733 43835179 ID=Msa0852640;Name=Msa0852640
chr5_4 mRNA 43835179 43838733 43835179 ID=Msa0852640-mRNA-1;Parent=Msa0852640;Name=Msa0852640-mRNA-1;_AED=0.12;_eAED=0.12;_QI=416|0.75|0.8|1|1|1|5|481|306
chr5_4 exon 43835179 43835615 43835179 ID=Msa0852640-mRNA-1:exon:13871;Parent=Msa0852640-mRNA-1
chr5_4 exon 43836043 43836256 43836043 ID=Msa0852640-mRNA-1:exon:13872;Parent=Msa0852640-mRNA-1
chr5_4 exon 43836363 43836535 43836363 ID=Msa0852640-mRNA-1:exon:13873;Parent=Msa0852640-mRNA-1
chr5_4 exon 43837027 43837069 43837027 ID=Msa0852640-mRNA-1:exon:13874;Parent=Msa0852640-mRNA-1
chr5_4 exon 43837783 43838733 43837783 ID=Msa0852640-mRNA-1:exon:13875;Parent=Msa0852640-mRNA-1
chr5_4 five_prime_UTR 43835179 43835594 43835179 ID=Msa0852640-mRNA-1:five_prime_utr;Parent=Msa0852640-mRNA-1
chr5_4 CDS 43835595 43835615 43835595 ID=Msa0852640-mRNA-1:cds;Parent=Msa0852640-mRNA-1
chr5_4 CDS 43836043 43836256 43836043 ID=Msa0852640-mRNA-1:cds;Parent=Msa0852640-mRNA-1
chr5_4 CDS 43836363 43836535 43836363 ID=Msa0852640-mRNA-1:cds;Parent=Msa0852640-mRNA-1
chr5_4 CDS 43837027 43837069 43837027 ID=Msa0852640-mRNA-1:cds;Parent=Msa0852640-mRNA-1
chr5_4 CDS 43837783 43838252 43837783 ID=Msa0852640-mRNA-1:cds;Parent=Msa0852640-mRNA-1
chr5_4 three_prime_UTR 43838253 43838733 43838253 ID=Msa0852640-mRNA-1:three_prime_utr;Parent=Msa0852640-mRNA-1
Gene Sequence

>Msa0852640

ATGGGTGAGGAAGCAAAACAGGAACAACCAAAGGTTGAGGAGAAGCAAGAGGAGAAGAAAGAAGAAAAGGCAGAAGGAGGAAAAGAAGAGGAAAAGAAGGAGGAAAGCAAAGAGGAAAAGCCAGAAGAAGAGAAAAAGGAAGAGCCTAAACCACCAGAACCATGTGTACTTTTTGTGGATTTGCATTGTGTTGGATGTGCAAAGAAGATTCAAAGATCTATAATGAAAATGAGAGGAGTTGAGGGAGTAGTTATTGACATGGCTAAAAATGAAGTTACCATAAAAGGTATAGTGGAGCCTCAAACTATATGCAATACAATTACCAAGAAAACAAAGAGAAGAGCAAATGTTATATCTCCATTGCCTCCAGCAGAAGGAGAACCTGTCCCAGAAGTTGTTAATTCTCAGGCTTGTGCTGAGCAACTCAAGAGAAAGATACTCCAAATGAGAGGAGTTCAAACAGCAGTAACAGAGTTTAGCACAGGGAAAGTAACTGTGACAGGAACAATGGATGCAAACAAGCTAGTGGATTATGTCTATAGAAGAACCAAAAAACAAGCAAAAATAGTTCCTAAACCAGAACCTGAACCAGAAAAGAAAGAAGAAGAAAGCAAAGAAGGTGAAAAACCTGCAGCAGAAGAAGAAGCTAAACCAGAAGAGAACAAGAAAGAAGAAGAGAAACCAACCGAGGAACCAAAGAAAGAAGAGGGGGGTGAGAATAAAGAAGAAAAGGGGGGTGAAGAAAGTAAAGAAGAAACCAAGAAAGAAGAGAGTGGTGCTACTGCTGTGGTTAATATTGATGAAGAAGGAATGAAAAGAATGATGTATTATTATCAATATCCTCCACTTTATGTGATTGAACGTACTCCACCTCCTCAACTATTCAGTGATGAAAATCCTAATGCATGCAGCATTTCATAA
Protein sequence

>Msa0852640

MGEEAKQEQPKVEEKQEEKKEEKAEGGKEEEKKEESKEEKPEEEKKEEPKPPEPCVLFVDLHCVGCAKKIQRSIMKMRGVEGVVIDMAKNEVTIKGIVEPQTICNTITKKTKRRANVISPLPPAEGEPVPEVVNSQACAEQLKRKILQMRGVQTAVTEFSTGKVTVTGTMDANKLVDYVYRRTKKQAKIVPKPEPEPEKKEEESKEGEKPAAEEEAKPEENKKEEEKPTEEPKKEEGGENKEEKGGEESKEETKKEESGATAVVNIDEEGMKRMMYYYQYPPLYVIERTPPPQLFSDENPNACSIS*