AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0857170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0857170 MtrunA17_Chr5g0441741 99.087 219 2 0 1 219 1 219 1.51e-164 452
Msa0857170 MtrunA17_Chr4g0064371 62.780 223 79 1 1 219 1 223 4.10e-111 317
Msa0857170 MtrunA17_Chr4g0026121 55.963 218 96 0 1 218 1 218 2.31e-94 275
Msa0857170 MtrunA17_Chr1g0169861 36.321 212 132 3 3 212 56 266 7.69e-49 160
Msa0857170 MtrunA17_Chr4g0010271 38.208 212 128 3 3 212 6 216 7.34e-48 156
Msa0857170 MtrunA17_Chr1g0210521 35.319 235 124 5 2 212 5 235 9.55e-47 154
Msa0857170 MtrunA17_Chr7g0237561 38.947 190 113 3 3 190 6 194 2.71e-45 150
Msa0857170 MtrunA17_Chr2g0296021 36.019 211 132 3 4 212 7 216 2.49e-44 147
Msa0857170 MtrunA17_Chr2g0291651 38.421 190 114 3 3 190 10 198 4.56e-43 144
Msa0857170 MtrunA17_Chr4g0043521 37.368 190 116 3 3 190 8 196 2.71e-42 142
Msa0857170 MtrunA17_Chr7g0237521 34.737 190 121 3 3 190 6 194 3.70e-36 126
Msa0857170 MtrunA17_Chr4g0010211 44.660 103 55 2 66 166 7 109 9.43e-24 91.3
Msa0857170 MtrunA17_Chr1g0169851 33.099 142 78 3 59 183 12 153 9.49e-22 87.4
Msa0857170 MtrunA17_Chr4g0058841 23.841 151 111 2 28 178 45 191 4.40e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0857170 AT5G11150.1 75.455 220 53 1 1 219 1 220 3.03e-135 379
Msa0857170 AT5G11150.2 75.455 220 53 1 1 219 1 220 5.08e-135 378
Msa0857170 AT4G32150.1 79.817 218 44 0 1 218 1 218 1.11e-129 364
Msa0857170 AT2G25340.1 77.982 218 48 0 1 218 1 218 2.31e-127 358
Msa0857170 AT5G22360.1 56.164 219 95 1 1 218 1 219 8.63e-98 284
Msa0857170 AT1G04750.1 37.264 212 130 3 3 212 6 216 1.58e-46 153
Msa0857170 AT1G04760.2 35.377 212 134 3 3 212 6 216 3.37e-46 152
Msa0857170 AT1G04760.1 35.377 212 134 3 3 212 6 216 3.37e-46 152
Msa0857170 AT2G33120.1 39.474 190 112 3 3 190 6 194 1.76e-45 150
Msa0857170 AT3G54300.3 34.188 234 128 5 2 212 5 235 5.25e-45 150
Msa0857170 AT3G54300.2 34.188 234 128 5 2 212 5 235 5.25e-45 150
Msa0857170 AT3G54300.1 34.188 234 128 5 2 212 5 235 5.25e-45 150
Msa0857170 AT2G32670.1 36.321 212 132 3 3 212 71 281 1.14e-43 148
Msa0857170 AT2G33120.2 37.374 198 113 4 3 190 6 202 2.40e-42 143
Msa0857170 AT4G15780.1 36.364 187 106 4 4 186 7 184 8.77e-36 125
Msa0857170 AT2G33110.1 34.419 215 128 7 3 212 6 212 5.29e-32 116
Msa0857170 AT2G33110.2 38.750 160 91 4 3 158 6 162 4.85e-30 110
Msa0857170 AT1G04750.2 34.058 138 89 2 77 212 1 138 3.89e-24 93.6
Msa0857170 AT1G04750.4 34.058 138 89 2 77 212 1 138 3.89e-24 93.6
Msa0857170 AT1G04750.3 34.058 138 89 2 77 212 1 138 3.89e-24 93.6
Msa0857170 AT2G33120.3 44.262 61 34 0 130 190 52 112 1.15e-12 63.5
Msa0857170 AT5G58060.1 22.727 154 115 2 28 181 45 194 4.20e-11 60.5

Find 69 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGCCTTGCAACAAATTTC+TGG 0.200994 5_4:+52497510 Msa0857170:three_prime_UTR
CCGTTATTCACGATTCAAAA+TGG 0.202220 5_4:+52493986 Msa0857170:exon
GCAACAAATTTCTGGTGTTT+TGG 0.204706 5_4:+52497518 Msa0857170:three_prime_UTR
GTTATCATTATTGTGTTATC+TGG 0.216320 5_4:-52493847 None:intergenic
TACGTGTGTATCGTTGTTTC+CGG 0.244994 5_4:-52494114 None:intergenic
AGAAAATATTGATAAAGTTT+TGG 0.259450 5_4:+52495649 Msa0857170:CDS
GATAGGATTAATCGGCTTAA+AGG 0.299245 5_4:+52494377 Msa0857170:CDS
CAGAGGTTTGTAAGGAGTTA+TGG 0.338003 5_4:+52494257 Msa0857170:CDS
AAAGTGTTGTTATAGCTTGA+TGG 0.353719 5_4:+52497669 Msa0857170:three_prime_UTR
AATATATGCATATAATTTAT+CGG 0.361766 5_4:-52497791 None:intergenic
GCAATTGAAATCAAAACAAA+TGG 0.381148 5_4:-52497459 None:intergenic
CATATAATTTATCGGGCAAC+TGG 0.390738 5_4:-52497783 None:intergenic
GTGTCCTTTCATATCATCTC+TGG 0.400774 5_4:+52497391 Msa0857170:three_prime_UTR
AGATTCATCAGAGGTTTGTA+AGG 0.404505 5_4:+52494249 Msa0857170:CDS
AGGTGATAGATTGGAATTGC+TGG 0.413308 5_4:+52495676 Msa0857170:CDS
TTTGGATAGAGGTGATAGAT+TGG 0.413502 5_4:+52495667 Msa0857170:CDS
CGCGCTGGTGGCGCGTGGAA+CGG 0.419354 5_4:+52494019 Msa0857170:CDS
ACGTGTGTATCGTTGTTTCC+GGG 0.420034 5_4:-52494113 None:intergenic
GGCGGCGTTTGTTGTTGTTC+CGG 0.443043 5_4:-52494060 None:intergenic
CGTTATTCACGATTCAAAAT+GGG 0.447571 5_4:+52493987 Msa0857170:exon
CCTATCAGCATTCGGATCAC+TGG 0.457235 5_4:-52494360 None:intergenic
CGAATGCTGATAGGATTAAT+CGG 0.459876 5_4:+52494369 Msa0857170:CDS
AGAACTGGAACAAACATCAT+TGG 0.465201 5_4:+52497631 Msa0857170:three_prime_UTR
ACCGTCTTGCTTCTAGTGAA+TGG 0.468398 5_4:+52497353 Msa0857170:exon
CCAGTGATCCGAATGCTGAT+AGG 0.476298 5_4:+52494360 Msa0857170:CDS
ATATATGCATATAATTTATC+GGG 0.479632 5_4:-52497790 None:intergenic
TGGACTCACGGTGCTGTGTA+TGG 0.483242 5_4:+52494181 Msa0857170:CDS
GAACTGGAACAAACATCATT+GGG 0.485467 5_4:+52497632 Msa0857170:three_prime_UTR
CAAACGCCGCCGCAATCGCG+CGG 0.490314 5_4:+52494072 Msa0857170:CDS
CGATTAATCCTATCAGCATT+CGG 0.491371 5_4:-52494368 None:intergenic
CTTCTATTTATCTTCAATTG+TGG 0.501020 5_4:+52497427 Msa0857170:three_prime_UTR
GTCGCTTTAGAAGCACTGTC+TGG 0.502008 5_4:+52495753 Msa0857170:CDS
TGTGATAGCATATGTTGTGT+TGG 0.514806 5_4:+52497305 Msa0857170:CDS
TAGAAGCAAGACGGTAGTGC+AGG 0.518587 5_4:-52497345 None:intergenic
CTCTACGCGCTGGTGGCGCG+TGG 0.524194 5_4:+52494014 Msa0857170:CDS
AACACCAGAAATTTGTTGCA+AGG 0.525105 5_4:-52497514 None:intergenic
GCTTAAAGGTGAAATGTCTC+AGG 0.529462 5_4:+52494391 Msa0857170:CDS
ATCTCTTCAAGAAACGCAAA+CGG 0.535896 5_4:-52494230 None:intergenic
GAGGATTTGCCGCGCGATTG+CGG 0.538753 5_4:-52494081 None:intergenic
CGTGTGTATCGTTGTTTCCG+GGG 0.552205 5_4:-52494112 None:intergenic
CAAATCCTCGAGAAAATCCC+CGG 0.556101 5_4:+52494095 Msa0857170:CDS
TTAGAAGCACTGTCTGGTGG+AGG 0.558410 5_4:+52495759 Msa0857170:CDS
CAATTGAAATCAAAACAAAT+GGG 0.562866 5_4:-52497458 None:intergenic
ACCTTTCAATGAGCTACGAC+GGG 0.566127 5_4:-52497748 None:intergenic
TTATAGCTTGATGGAGTCAT+CGG 0.567492 5_4:+52497678 Msa0857170:three_prime_UTR
ATTTATCTTCAATTGTGGCG+TGG 0.571192 5_4:+52497432 Msa0857170:three_prime_UTR
GCTGGTGGCGCGTGGAACGG+TGG 0.578906 5_4:+52494022 Msa0857170:CDS
GATAAAGCTGCTAATATGCA+AGG 0.579433 5_4:+52495701 Msa0857170:CDS
CAACATATGCTATCACAAGT+AGG 0.588389 5_4:-52497299 None:intergenic
GATTTGCCGCGCGATTGCGG+CGG 0.588839 5_4:-52494078 None:intergenic
GCTTTAGAAGCACTGTCTGG+TGG 0.589136 5_4:+52495756 Msa0857170:CDS
TGATAGATTGGAATTGCTGG+TGG 0.593278 5_4:+52495679 Msa0857170:CDS
AAGACGGTAGTGCAGGCCCA+TGG 0.602690 5_4:-52497338 None:intergenic
TCCCGTCGTAGCTCATTGAA+AGG 0.606260 5_4:+52497747 Msa0857170:three_prime_UTR
GAAACGAACCGATGGACTCA+CGG 0.607839 5_4:+52494169 Msa0857170:CDS
CAAGCCAGAGATGATATGAA+AGG 0.608146 5_4:-52497395 None:intergenic
ACACAGCACCGTGAGTCCAT+CGG 0.624165 5_4:-52494177 None:intergenic
GACCTTTCAATGAGCTACGA+CGG 0.624182 5_4:-52497749 None:intergenic
TTTCACGTGAAACGAACCGA+TGG 0.628828 5_4:+52494161 Msa0857170:CDS
ACGTATCGTATTCACAAGAT+CGG 0.630773 5_4:+52494132 Msa0857170:CDS
GTGGTTCTAGCAGAGTTCAC+CGG 0.634337 5_4:+52494041 Msa0857170:CDS
GGGTATACTCTACGCGCTGG+TGG 0.634486 5_4:+52494007 Msa0857170:CDS
AATGGGTATACTCTACGCGC+TGG 0.637918 5_4:+52494004 Msa0857170:exon
TGTAAGGAGTTATGGACGTG+CGG 0.656982 5_4:+52494265 Msa0857170:CDS
TTCTTGAAGAGATTCATCAG+AGG 0.658130 5_4:+52494240 Msa0857170:CDS
ACCATTCACTAGAAGCAAGA+CGG 0.663304 5_4:-52497354 None:intergenic
TGGCGGATGATAGCGTTGGA+CGG 0.663803 5_4:+52494201 Msa0857170:CDS
TGTATGGCGGATGATAGCGT+TGG 0.668509 5_4:+52494197 Msa0857170:CDS
ACTCACGGTGCTGTGTATGG+CGG 0.734585 5_4:+52494184 Msa0857170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTCTTACAAATAAAA+AGG - chr5_4:52497072-52497091 None:intergenic 10.0%
!! TAAATAATTCAAAAAGAAAA+TGG - chr5_4:52497708-52497727 None:intergenic 10.0%
!! AATATATGCATATAATTTAT+CGG - chr5_4:52497794-52497813 None:intergenic 10.0%
!!! AAATTGATTTTGATATGTTT+GGG + chr5_4:52494784-52494803 Msa0857170:intron 15.0%
!! AATATAATAACAACATAGAT+TGG + chr5_4:52495312-52495331 Msa0857170:intron 15.0%
!!! AGAAAATATTGATAAAGTTT+TGG + chr5_4:52495649-52495668 Msa0857170:CDS 15.0%
!! ATAATATAACACCAAAAAAT+TGG + chr5_4:52497606-52497625 Msa0857170:three_prime_UTR 15.0%
!! ATATATGCATATAATTTATC+GGG - chr5_4:52497793-52497812 None:intergenic 15.0%
!!! TATGAATGATGAATTTTCTA+GGG + chr5_4:52494310-52494329 Msa0857170:CDS 20.0%
!!! TAACATCTTTTTATTAAACC+GGG - chr5_4:52494516-52494535 None:intergenic 20.0%
!!! GTAACATCTTTTTATTAAAC+CGG - chr5_4:52494517-52494536 None:intergenic 20.0%
!!! GAAATTGATTTTGATATGTT+TGG + chr5_4:52494783-52494802 Msa0857170:intron 20.0%
!!! AATTGATTTTGATATGTTTG+GGG + chr5_4:52494785-52494804 Msa0857170:intron 20.0%
!!! AAAATCAATTTTAAATCTGC+AGG - chr5_4:52494910-52494929 None:intergenic 20.0%
!! GTTGGAAATATATAGAAAAA+TGG + chr5_4:52495205-52495224 Msa0857170:intron 20.0%
!!! GACAAAGATTTTATCTTTAT+AGG - chr5_4:52495907-52495926 None:intergenic 20.0%
!!! TCTGTTTTAATTATCATTCT+TGG + chr5_4:52495967-52495986 Msa0857170:intron 20.0%
!! AGGAAATTAAAATATTGTTG+TGG - chr5_4:52496599-52496618 None:intergenic 20.0%
!!! TTTTTGAGGTTATAATAAGA+AGG - chr5_4:52496619-52496638 None:intergenic 20.0%
!! GAATAAAATATACAACACTT+TGG - chr5_4:52496697-52496716 None:intergenic 20.0%
!!! AATTTTGAAATTTCATGTCA+TGG + chr5_4:52497136-52497155 Msa0857170:intron 20.0%
!! CAATTGAAATCAAAACAAAT+GGG - chr5_4:52497461-52497480 None:intergenic 20.0%
! GTTATCATTATTGTGTTATC+TGG - chr5_4:52493850-52493869 None:intergenic 25.0%
!! CTATGAATGATGAATTTTCT+AGG + chr5_4:52494309-52494328 Msa0857170:CDS 25.0%
! GTTGAAAATTTGAATTTACC+CGG + chr5_4:52494495-52494514 Msa0857170:intron 25.0%
! AATTAAGTGTATGTTTCGAT+TGG + chr5_4:52494576-52494595 Msa0857170:intron 25.0%
! AAATAGAAACAAACACACTT+AGG + chr5_4:52495131-52495150 Msa0857170:intron 25.0%
! CATTATTCTGATTGAACTTA+AGG - chr5_4:52495548-52495567 None:intergenic 25.0%
! GAATAATGTTTGTCAAACTT+AGG + chr5_4:52495560-52495579 Msa0857170:intron 25.0%
!!! GTTTCTATTTTTTTCCTTAC+AGG + chr5_4:52495610-52495629 Msa0857170:intron 25.0%
!!! ATTGATAAAGTTTTGGATAG+AGG + chr5_4:52495656-52495675 Msa0857170:CDS 25.0%
! CTTTATAGGTTCAACTTATA+TGG - chr5_4:52495893-52495912 None:intergenic 25.0%
! AAGATAAAATCTTTGTCTCT+TGG + chr5_4:52495910-52495929 Msa0857170:intron 25.0%
! AGATAAAATCTTTGTCTCTT+GGG + chr5_4:52495911-52495930 Msa0857170:intron 25.0%
! AATTAAAACAGACGAAGAAA+AGG - chr5_4:52495959-52495978 None:intergenic 25.0%
!! ATAGGATTTGTCAATTTTCA+TGG + chr5_4:52495998-52496017 Msa0857170:intron 25.0%
! TCACATTTATTAATCTCTGA+GGG - chr5_4:52496051-52496070 None:intergenic 25.0%
! ATCACATTTATTAATCTCTG+AGG - chr5_4:52496052-52496071 None:intergenic 25.0%
!! TCATTTTATCGTTTATCTTC+CGG - chr5_4:52496366-52496385 None:intergenic 25.0%
!!! TTGAGATTTTGTTTTTGGAA+AGG - chr5_4:52496565-52496584 None:intergenic 25.0%
! AAAAAACATCAGTAGAATCA+AGG + chr5_4:52496954-52496973 Msa0857170:intron 25.0%
! CTTCTATTTATCTTCAATTG+TGG + chr5_4:52497427-52497446 Msa0857170:three_prime_UTR 25.0%
! GCAATTGAAATCAAAACAAA+TGG - chr5_4:52497462-52497481 None:intergenic 25.0%
! CCATTTTGAATCGTGAATAA+CGG - chr5_4:52493989-52494008 None:intergenic 30.0%
CGTTATTCACGATTCAAAAT+GGG + chr5_4:52493987-52494006 Msa0857170:exon 30.0%
! AGCTTTTGTCAAGATTATGA+TGG + chr5_4:52494660-52494679 Msa0857170:intron 30.0%
GTTGTTCGTAAGTTTATGTT+TGG - chr5_4:52495095-52495114 None:intergenic 30.0%
GAACTCAAAAATTGTATTGG+GGG - chr5_4:52495382-52495401 None:intergenic 30.0%
GGAACTCAAAAATTGTATTG+GGG - chr5_4:52495383-52495402 None:intergenic 30.0%
GGGAACTCAAAAATTGTATT+GGG - chr5_4:52495384-52495403 None:intergenic 30.0%
ATAAAAAATCCCCCTCATTT+GGG - chr5_4:52495406-52495425 None:intergenic 30.0%
AATAAAAAATCCCCCTCATT+TGG - chr5_4:52495407-52495426 None:intergenic 30.0%
AAGAAATAGAAGACATTGAG+AGG - chr5_4:52495461-52495480 None:intergenic 30.0%
!! TTGAACTTAAGGCTTTGTTT+GGG - chr5_4:52495537-52495556 None:intergenic 30.0%
!! ATTGAACTTAAGGCTTTGTT+TGG - chr5_4:52495538-52495557 None:intergenic 30.0%
CATAACATTTCTTACCTGTA+AGG - chr5_4:52495627-52495646 None:intergenic 30.0%
GTTCTACTGGGAAAAAAATT+CGG - chr5_4:52496223-52496242 None:intergenic 30.0%
! AACAGCAAAATAGTTCTACT+GGG - chr5_4:52496235-52496254 None:intergenic 30.0%
! TAACAGCAAAATAGTTCTAC+TGG - chr5_4:52496236-52496255 None:intergenic 30.0%
! GTAATTTTTCGTTGCATATC+TGG + chr5_4:52496307-52496326 Msa0857170:intron 30.0%
! TCAGCACATTGTTTGTTAAA+GGG - chr5_4:52496343-52496362 None:intergenic 30.0%
TTCCATACAGTTTCAAATTG+CGG + chr5_4:52496391-52496410 Msa0857170:intron 30.0%
TGAAATTGAGACAATGAACT+AGG + chr5_4:52496648-52496667 Msa0857170:intron 30.0%
!! GAATTCTAAAGCTCTTTCTA+AGG - chr5_4:52496864-52496883 None:intergenic 30.0%
!! AGAGCTTTAGAATTCCAAAT+TGG + chr5_4:52496869-52496888 Msa0857170:intron 30.0%
TAATTGAATTCTTACCAGAC+AGG - chr5_4:52497112-52497131 None:intergenic 30.0%
!!! ATGGTGATTGTTTTTAGTAG+TGG + chr5_4:52497155-52497174 Msa0857170:intron 30.0%
!!! TTTTGATTTCAATTGCTGCT+GGG + chr5_4:52497466-52497485 Msa0857170:three_prime_UTR 30.0%
!!! CAACAAATTTCTGGTGTTTT+GGG + chr5_4:52497519-52497538 Msa0857170:three_prime_UTR 30.0%
! TTTTCATTACTGAGGTTTGA+AGG + chr5_4:52497548-52497567 Msa0857170:three_prime_UTR 30.0%
!! AAAGTGTTGTTATAGCTTGA+TGG + chr5_4:52497669-52497688 Msa0857170:three_prime_UTR 30.0%
CCGTTATTCACGATTCAAAA+TGG + chr5_4:52493986-52494005 Msa0857170:exon 35.0%
ACGTATCGTATTCACAAGAT+CGG + chr5_4:52494132-52494151 Msa0857170:CDS 35.0%
ATCTCTTCAAGAAACGCAAA+CGG - chr5_4:52494233-52494252 None:intergenic 35.0%
TTCTTGAAGAGATTCATCAG+AGG + chr5_4:52494240-52494259 Msa0857170:CDS 35.0%
AGATTCATCAGAGGTTTGTA+AGG + chr5_4:52494249-52494268 Msa0857170:CDS 35.0%
CGATTAATCCTATCAGCATT+CGG - chr5_4:52494371-52494390 None:intergenic 35.0%
CGAATGCTGATAGGATTAAT+CGG + chr5_4:52494369-52494388 Msa0857170:CDS 35.0%
! GATAGGATTAATCGGCTTAA+AGG + chr5_4:52494377-52494396 Msa0857170:CDS 35.0%
!!! CAGTGAGTTTTGAGAAATCA+CGG + chr5_4:52494599-52494618 Msa0857170:intron 35.0%
! TGCGTGTTTGGATTCATTTT+TGG + chr5_4:52494728-52494747 Msa0857170:intron 35.0%
!! GCGTGTTTGGATTCATTTTT+GGG + chr5_4:52494729-52494748 Msa0857170:intron 35.0%
AGTCAAAAGTGATTCTGTAG+TGG + chr5_4:52494754-52494773 Msa0857170:intron 35.0%
AAGTCAGAATCAAACTCTAG+AGG - chr5_4:52494989-52495008 None:intergenic 35.0%
GATGTAAAAAACGCGTTTAG+AGG - chr5_4:52495035-52495054 None:intergenic 35.0%
!! AAACACACTTAGGCTTTGTT+TGG + chr5_4:52495141-52495160 Msa0857170:intron 35.0%
!! AACACACTTAGGCTTTGTTT+GGG + chr5_4:52495142-52495161 Msa0857170:intron 35.0%
TCAAAAATTGTATTGGGGGA+GGG - chr5_4:52495378-52495397 None:intergenic 35.0%
GGGGAACTCAAAAATTGTAT+TGG - chr5_4:52495385-52495404 None:intergenic 35.0%
TAAAAAATCCCCCTCATTTG+GGG - chr5_4:52495405-52495424 None:intergenic 35.0%
TTTGGATAGAGGTGATAGAT+TGG + chr5_4:52495667-52495686 Msa0857170:CDS 35.0%
GATAAAGCTGCTAATATGCA+AGG + chr5_4:52495701-52495720 Msa0857170:CDS 35.0%
TCATTCTTGGCATGATCAAT+AGG + chr5_4:52495980-52495999 Msa0857170:intron 35.0%
ACTATCTTCAGCTTAAGCTT+AGG + chr5_4:52496140-52496159 Msa0857170:intron 35.0%
! GTCAGCACATTGTTTGTTAA+AGG - chr5_4:52496344-52496363 None:intergenic 35.0%
! TTAACAAACAATGTGCTGAC+CGG + chr5_4:52496344-52496363 Msa0857170:intron 35.0%
TCCATACAGTTTCAAATTGC+GGG + chr5_4:52496392-52496411 Msa0857170:intron 35.0%
! TCATATTTTTCACCGCATTG+CGG - chr5_4:52496487-52496506 None:intergenic 35.0%
!!! TAGTTTTGACCGCATTGAAT+TGG - chr5_4:52496518-52496537 None:intergenic 35.0%
!!! GCGGTTTGAGATTTTGTTTT+TGG - chr5_4:52496570-52496589 None:intergenic 35.0%
AATATTGTTGTGGCAATGTG+CGG - chr5_4:52496589-52496608 None:intergenic 35.0%
!!! TTTCAACACACTGGTTTTTG+AGG - chr5_4:52496633-52496652 None:intergenic 35.0%
TTGTCTCAATTTCAACACAC+TGG - chr5_4:52496642-52496661 None:intergenic 35.0%
! TGCTGCAAAGTTTGAAACTT+AGG + chr5_4:52496751-52496770 Msa0857170:intron 35.0%
TTGAAACTTAGGTTTACACG+AGG + chr5_4:52496762-52496781 Msa0857170:intron 35.0%
TGAAACTTAGGTTTACACGA+GGG + chr5_4:52496763-52496782 Msa0857170:intron 35.0%
CTCTTTGTAGGAGAATTCAA+AGG + chr5_4:52496908-52496927 Msa0857170:intron 35.0%
AAACTGAAAGCAAGATTTGC+TGG + chr5_4:52496931-52496950 Msa0857170:intron 35.0%
GATCGATGAATTGTAATCTG+AGG - chr5_4:52497227-52497246 None:intergenic 35.0%
CAACATATGCTATCACAAGT+AGG - chr5_4:52497302-52497321 None:intergenic 35.0%
TGTGATAGCATATGTTGTGT+TGG + chr5_4:52497305-52497324 Msa0857170:CDS 35.0%
ATTTATCTTCAATTGTGGCG+TGG + chr5_4:52497432-52497451 Msa0857170:three_prime_UTR 35.0%
!!! GTTTTGATTTCAATTGCTGC+TGG + chr5_4:52497465-52497484 Msa0857170:three_prime_UTR 35.0%
AAAGCCTTGCAACAAATTTC+TGG + chr5_4:52497510-52497529 Msa0857170:three_prime_UTR 35.0%
!! AACACCAGAAATTTGTTGCA+AGG - chr5_4:52497517-52497536 None:intergenic 35.0%
!!! GCAACAAATTTCTGGTGTTT+TGG + chr5_4:52497518-52497537 Msa0857170:three_prime_UTR 35.0%
! GGTGGTATTTTTCATTACTG+AGG + chr5_4:52497540-52497559 Msa0857170:three_prime_UTR 35.0%
ACCAAAAAATTGGAGAGAAC+TGG + chr5_4:52497616-52497635 Msa0857170:three_prime_UTR 35.0%
!!! TCCAGTTCTCTCCAATTTTT+TGG - chr5_4:52497620-52497639 None:intergenic 35.0%
AGAACTGGAACAAACATCAT+TGG + chr5_4:52497631-52497650 Msa0857170:three_prime_UTR 35.0%
GAACTGGAACAAACATCATT+GGG + chr5_4:52497632-52497651 Msa0857170:three_prime_UTR 35.0%
TTATAGCTTGATGGAGTCAT+CGG + chr5_4:52497678-52497697 Msa0857170:three_prime_UTR 35.0%
CATATAATTTATCGGGCAAC+TGG - chr5_4:52497786-52497805 None:intergenic 35.0%
! TACGTGTGTATCGTTGTTTC+CGG - chr5_4:52494117-52494136 None:intergenic 40.0%
CAGAGGTTTGTAAGGAGTTA+TGG + chr5_4:52494257-52494276 Msa0857170:CDS 40.0%
!!! TAGGGTTTTGAATCAGCAGA+TGG + chr5_4:52494328-52494347 Msa0857170:CDS 40.0%
GCTTAAAGGTGAAATGTCTC+AGG + chr5_4:52494391-52494410 Msa0857170:CDS 40.0%
!!! TGAGTTTTGAGAAATCACGG+TGG + chr5_4:52494602-52494621 Msa0857170:intron 40.0%
GTTTGGACTGACAGTGAATT+TGG - chr5_4:52494701-52494720 None:intergenic 40.0%
CAAACACGCACACATATGTT+TGG - chr5_4:52494718-52494737 None:intergenic 40.0%
CAAACATATGTGTGCGTGTT+TGG + chr5_4:52494716-52494735 Msa0857170:intron 40.0%
! GTAGCTTTTGCTTGCAATCA+TGG + chr5_4:52494864-52494883 Msa0857170:intron 40.0%
!! TTAGGCTTTGTTTGGGAGTT+TGG + chr5_4:52495149-52495168 Msa0857170:intron 40.0%
CTCAAAAATTGTATTGGGGG+AGG - chr5_4:52495379-52495398 None:intergenic 40.0%
!!! TTTTTGAGTTCCCCCAAATG+AGG + chr5_4:52495391-52495410 Msa0857170:intron 40.0%
!!! TTTTGAGTTCCCCCAAATGA+GGG + chr5_4:52495392-52495411 Msa0857170:intron 40.0%
AAAAAATCCCCCTCATTTGG+GGG - chr5_4:52495404-52495423 None:intergenic 40.0%
TAGAAGACATTGAGAGGAGA+GGG - chr5_4:52495455-52495474 None:intergenic 40.0%
ATAGAAGACATTGAGAGGAG+AGG - chr5_4:52495456-52495475 None:intergenic 40.0%
!!! GAGGGTTTTGGAAAAAAGGA+AGG - chr5_4:52495493-52495512 None:intergenic 40.0%
!! TAAGGCTTTGTTTGGGAGTT+TGG - chr5_4:52495530-52495549 None:intergenic 40.0%
AGGTGATAGATTGGAATTGC+TGG + chr5_4:52495676-52495695 Msa0857170:CDS 40.0%
TGATAGATTGGAATTGCTGG+TGG + chr5_4:52495679-52495698 Msa0857170:CDS 40.0%
! ATTAATCTCTGAGGGTTCGA+AGG - chr5_4:52496043-52496062 None:intergenic 40.0%
ATCTTCAGCTTAAGCTTAGG+TGG + chr5_4:52496143-52496162 Msa0857170:intron 40.0%
AACTAGTCAGAGTTAGAGAG+AGG + chr5_4:52496281-52496300 Msa0857170:intron 40.0%
ACTAGTCAGAGTTAGAGAGA+GGG + chr5_4:52496282-52496301 Msa0857170:intron 40.0%
! ACCCGCAATTTGAAACTGTA+TGG - chr5_4:52496396-52496415 None:intergenic 40.0%
! CATATTTTTCACCGCATTGC+GGG - chr5_4:52496486-52496505 None:intergenic 40.0%
ATCCCATGACCAATTCAATG+CGG + chr5_4:52496506-52496525 Msa0857170:intron 40.0%
AAACTTACGCTGCTAAGCAA+TGG + chr5_4:52496719-52496738 Msa0857170:intron 40.0%
ATTCCAAATTGGCGTATCCT+AGG + chr5_4:52496880-52496899 Msa0857170:intron 40.0%
TCCTAGGCAATTCTCTTTGT+AGG + chr5_4:52496896-52496915 Msa0857170:intron 40.0%
TCCTACAAAGAGAATTGCCT+AGG - chr5_4:52496900-52496919 None:intergenic 40.0%
ACAAAATGCTTCCATGTTGC+AGG + chr5_4:52497259-52497278 Msa0857170:intron 40.0%
ATAAGTGCAATCCTGCAACA+TGG - chr5_4:52497273-52497292 None:intergenic 40.0%
!!! TGTTGGCTTTTGTTTGCCAT+GGG + chr5_4:52497322-52497341 Msa0857170:CDS 40.0%
ACCATTCACTAGAAGCAAGA+CGG - chr5_4:52497357-52497376 None:intergenic 40.0%
GTGTCCTTTCATATCATCTC+TGG + chr5_4:52497391-52497410 Msa0857170:three_prime_UTR 40.0%
CAAGCCAGAGATGATATGAA+AGG - chr5_4:52497398-52497417 None:intergenic 40.0%
!!! CAAATTTCTGGTGTTTTGGG+TGG + chr5_4:52497522-52497541 Msa0857170:three_prime_UTR 40.0%
CAAATCCTCGAGAAAATCCC+CGG + chr5_4:52494095-52494114 Msa0857170:CDS 45.0%
! ACGTGTGTATCGTTGTTTCC+GGG - chr5_4:52494116-52494135 None:intergenic 45.0%
TTTCACGTGAAACGAACCGA+TGG + chr5_4:52494161-52494180 Msa0857170:CDS 45.0%
TGTAAGGAGTTATGGACGTG+CGG + chr5_4:52494265-52494284 Msa0857170:CDS 45.0%
!! GCTTTGTTTGGGAGTTTGGA+GGG + chr5_4:52495153-52495172 Msa0857170:intron 45.0%
! CTTTGTTTGGGAGTTTGGAG+GGG + chr5_4:52495154-52495173 Msa0857170:intron 45.0%
! TTGTATTGGGGGAGGGTTTT+GGG - chr5_4:52495371-52495390 None:intergenic 45.0%
! ATTGTATTGGGGGAGGGTTT+TGG - chr5_4:52495372-52495391 None:intergenic 45.0%
! TTTGAGTTCCCCCAAATGAG+GGG + chr5_4:52495393-52495412 Msa0857170:intron 45.0%
GGAAAAAAGGAAGGAGCAAG+TGG - chr5_4:52495484-52495503 None:intergenic 45.0%
!!! AGGGGAGGGTTTTGGAAAAA+AGG - chr5_4:52495497-52495516 None:intergenic 45.0%
! CTTTGTTTGGGAGTTTGGAG+GGG - chr5_4:52495154-52495173 None:intergenic 45.0%
!! GCTTTGTTTGGGAGTTTGGA+GGG - chr5_4:52495153-52495172 None:intergenic 45.0%
CAAACTTAGGAATGTGCCAG+TGG + chr5_4:52495573-52495592 Msa0857170:intron 45.0%
TTCAGCTTAAGCTTAGGTGG+TGG + chr5_4:52496146-52496165 Msa0857170:intron 45.0%
TCAGCTTAAGCTTAGGTGGT+GGG + chr5_4:52496147-52496166 Msa0857170:intron 45.0%
GACCGCATTGAATTGGTCAT+GGG - chr5_4:52496511-52496530 None:intergenic 45.0%
TGACCGCATTGAATTGGTCA+TGG - chr5_4:52496512-52496531 None:intergenic 45.0%
ACTATCACCCTTGTAGATGC+AGG + chr5_4:52496534-52496553 Msa0857170:intron 45.0%
CTATCACCCTTGTAGATGCA+GGG + chr5_4:52496535-52496554 Msa0857170:intron 45.0%
GGAATACCCTGCATCTACAA+GGG - chr5_4:52496544-52496563 None:intergenic 45.0%
AGGAATACCCTGCATCTACA+AGG - chr5_4:52496545-52496564 None:intergenic 45.0%
!! GGTCGAAGCTTTTGTTTCGA+CGG - chr5_4:52496843-52496862 None:intergenic 45.0%
TTGCCTAGGATACGCCAATT+TGG - chr5_4:52496886-52496905 None:intergenic 45.0%
TTAAAGCAAGTGTGCCTGTC+TGG + chr5_4:52497095-52497114 Msa0857170:intron 45.0%
!!! GTGTTGGCTTTTGTTTGCCA+TGG + chr5_4:52497321-52497340 Msa0857170:CDS 45.0%
ACCGTCTTGCTTCTAGTGAA+TGG + chr5_4:52497353-52497372 Msa0857170:exon 45.0%
ACCTTTCAATGAGCTACGAC+GGG - chr5_4:52497751-52497770 None:intergenic 45.0%
GACCTTTCAATGAGCTACGA+CGG - chr5_4:52497752-52497771 None:intergenic 45.0%
!! TAAAAAAAAAAACTTATAAA+CGG - chr5_4:52495861-52495880 None:intergenic 5.0%
AATGGGTATACTCTACGCGC+TGG + chr5_4:52494004-52494023 Msa0857170:exon 50.0%
!! GTGGTTCTAGCAGAGTTCAC+CGG + chr5_4:52494041-52494060 Msa0857170:CDS 50.0%
! TGTTTCCGGGGATTTTCTCG+AGG - chr5_4:52494103-52494122 None:intergenic 50.0%
! CGTGTGTATCGTTGTTTCCG+GGG - chr5_4:52494115-52494134 None:intergenic 50.0%
! GAAACGAACCGATGGACTCA+CGG + chr5_4:52494169-52494188 Msa0857170:CDS 50.0%
!! TGTATGGCGGATGATAGCGT+TGG + chr5_4:52494197-52494216 Msa0857170:CDS 50.0%
CCTATCAGCATTCGGATCAC+TGG - chr5_4:52494363-52494382 None:intergenic 50.0%
CCAGTGATCCGAATGCTGAT+AGG + chr5_4:52494360-52494379 Msa0857170:CDS 50.0%
!! GGCTTTGTTTGGGAGTTTGG+AGG + chr5_4:52495152-52495171 Msa0857170:intron 50.0%
! TGTATTGGGGGAGGGTTTTG+GGG - chr5_4:52495370-52495389 None:intergenic 50.0%
TTGAGTTCCCCCAAATGAGG+GGG + chr5_4:52495394-52495413 Msa0857170:intron 50.0%
ACATTGAGAGGAGAGGGCTT+TGG - chr5_4:52495449-52495468 None:intergenic 50.0%
!! GGCTTTGTTTGGGAGTTTGG+AGG - chr5_4:52495152-52495171 None:intergenic 50.0%
ACCTGAGCAAAGACAACCAC+TGG - chr5_4:52495592-52495611 None:intergenic 50.0%
!! GTCGCTTTAGAAGCACTGTC+TGG + chr5_4:52495753-52495772 Msa0857170:CDS 50.0%
!! GCTTTAGAAGCACTGTCTGG+TGG + chr5_4:52495756-52495775 Msa0857170:CDS 50.0%
!! TTAGAAGCACTGTCTGGTGG+AGG + chr5_4:52495759-52495778 Msa0857170:CDS 50.0%
CAGCTTAAGCTTAGGTGGTG+GGG + chr5_4:52496148-52496167 Msa0857170:intron 50.0%
GGGGATTGAGTAGAGGTTCT+AGG + chr5_4:52496167-52496186 Msa0857170:intron 50.0%
CAGTTTCAAATTGCGGGTCG+TGG + chr5_4:52496398-52496417 Msa0857170:intron 50.0%
TCACAATTGTGCCCGCAATG+CGG + chr5_4:52496472-52496491 Msa0857170:intron 50.0%
TAGAAGCAAGACGGTAGTGC+AGG - chr5_4:52497348-52497367 None:intergenic 50.0%
TCCCGTCGTAGCTCATTGAA+AGG + chr5_4:52497747-52497766 Msa0857170:three_prime_UTR 50.0%
!! GGCGGCGTTTGTTGTTGTTC+CGG - chr5_4:52494063-52494082 None:intergenic 55.0%
ACACAGCACCGTGAGTCCAT+CGG - chr5_4:52494180-52494199 None:intergenic 55.0%
!! TGGACTCACGGTGCTGTGTA+TGG + chr5_4:52494181-52494200 Msa0857170:CDS 55.0%
!! ACTCACGGTGCTGTGTATGG+CGG + chr5_4:52494184-52494203 Msa0857170:CDS 55.0%
! TGGCGGATGATAGCGTTGGA+CGG + chr5_4:52494201-52494220 Msa0857170:CDS 55.0%
!!! TGGACGTGCGGTTTTGACTG+CGG + chr5_4:52494277-52494296 Msa0857170:CDS 55.0%
TGTTTGGGAGTTTGGAGGGG+AGG + chr5_4:52495157-52495176 Msa0857170:intron 55.0%
GTTTGGGAGTTTGGAGGGGA+GGG + chr5_4:52495158-52495177 Msa0857170:intron 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG + chr5_4:52495159-52495178 Msa0857170:intron 55.0%
!!! GGGTTTTGGGGGGTTATGGA+GGG - chr5_4:52495358-52495377 None:intergenic 55.0%
!!! AGGGTTTTGGGGGGTTATGG+AGG - chr5_4:52495359-52495378 None:intergenic 55.0%
!!! TATTGGGGGAGGGTTTTGGG+GGG - chr5_4:52495368-52495387 None:intergenic 55.0%
!! GTATTGGGGGAGGGTTTTGG+GGG - chr5_4:52495369-52495388 None:intergenic 55.0%
! TTGAGAGGAGAGGGCTTTGG+AGG - chr5_4:52495446-52495465 None:intergenic 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG - chr5_4:52495159-52495178 None:intergenic 55.0%
GTTTGGGAGTTTGGAGGGGA+GGG - chr5_4:52495158-52495177 None:intergenic 55.0%
TGTTTGGGAGTTTGGAGGGG+AGG - chr5_4:52495157-52495176 None:intergenic 55.0%
GCCAGTGGTTGTCTTTGCTC+AGG + chr5_4:52495588-52495607 Msa0857170:intron 55.0%
!! AGGTGGTGGGGATTGAGTAG+AGG + chr5_4:52496160-52496179 Msa0857170:intron 55.0%
GGGTATACTCTACGCGCTGG+TGG + chr5_4:52494007-52494026 Msa0857170:CDS 60.0%
GAGGATTTGCCGCGCGATTG+CGG - chr5_4:52494084-52494103 None:intergenic 60.0%
GAGTTTGGAGGGGAGGGGAA+GGG + chr5_4:52495164-52495183 Msa0857170:intron 60.0%
!!! GGGAGGGTTTTGGGGGGTTA+TGG - chr5_4:52495362-52495381 None:intergenic 60.0%
CTTGGGTCGAGCGAAGCTGT+TGG + chr5_4:52495928-52495947 Msa0857170:intron 60.0%
AAGACGGTAGTGCAGGCCCA+TGG - chr5_4:52497341-52497360 None:intergenic 60.0%
GATTTGCCGCGCGATTGCGG+CGG - chr5_4:52494081-52494100 None:intergenic 65.0%
GGAGTTTGGAGGGGAGGGGA+AGG + chr5_4:52495163-52495182 Msa0857170:intron 65.0%
TTTGGAGGGGAGGGGAAGGG+AGG + chr5_4:52495167-52495186 Msa0857170:intron 65.0%
TTGGAGGGGAGGGGAAGGGA+GGG + chr5_4:52495168-52495187 Msa0857170:intron 65.0%
GGAAGGGAGGGGAGGGCTTT+GGG + chr5_4:52495180-52495199 Msa0857170:intron 65.0%
GAAGGGAGGGGAGGGCTTTG+GGG + chr5_4:52495181-52495200 Msa0857170:intron 65.0%
! AAGGGAGGGGAGGGCTTTGG+GGG + chr5_4:52495182-52495201 Msa0857170:intron 65.0%
GAGTTTGGAGGGGAGGGGAG+GGG - chr5_4:52495515-52495534 None:intergenic 65.0%
GGAGTTTGGAGGGGAGGGGA+GGG - chr5_4:52495163-52495182 None:intergenic 65.0%
CAAACGCCGCCGCAATCGCG+CGG + chr5_4:52494072-52494091 Msa0857170:CDS 70.0%
TGGAGGGGAGGGGAAGGGAG+GGG + chr5_4:52495169-52495188 Msa0857170:intron 70.0%
GGGAAGGGAGGGGAGGGCTT+TGG + chr5_4:52495179-52495198 Msa0857170:intron 70.0%
!! AGGGAGGGGAGGGCTTTGGG+GGG + chr5_4:52495183-52495202 Msa0857170:intron 70.0%
!! AGGGGAGGGCTTTGGGGGGT+TGG + chr5_4:52495187-52495206 Msa0857170:intron 70.0%
! GGGAGGGGAGGGGAGGGTTT+TGG - chr5_4:52495505-52495524 None:intergenic 70.0%
TTGGAGGGGAGGGGAGGGGA+GGG - chr5_4:52495511-52495530 None:intergenic 70.0%
TTTGGAGGGGAGGGGAGGGG+AGG - chr5_4:52495512-52495531 None:intergenic 70.0%
GGGAGTTTGGAGGGGAGGGG+AGG - chr5_4:52495162-52495181 None:intergenic 70.0%
! CTCTACGCGCTGGTGGCGCG+TGG + chr5_4:52494014-52494033 Msa0857170:CDS 75.0%
! CGCGCTGGTGGCGCGTGGAA+CGG + chr5_4:52494019-52494038 Msa0857170:CDS 75.0%
! GCTGGTGGCGCGTGGAACGG+TGG + chr5_4:52494022-52494041 Msa0857170:CDS 75.0%
AGGGGAGGGGAAGGGAGGGG+AGG + chr5_4:52495172-52495191 Msa0857170:intron 75.0%
GGGGAGGGGAAGGGAGGGGA+GGG + chr5_4:52495173-52495192 Msa0857170:intron 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 52493840 52497836 52493840 ID=Msa0857170;Name=Msa0857170
chr5_4 mRNA 52493840 52497836 52493840 ID=Msa0857170-mRNA-1;Parent=Msa0857170;Name=Msa0857170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=165|1|1|1|1|1|3|468|219
chr5_4 exon 52493840 52494412 52493840 ID=Msa0857170-mRNA-1:exon:16388;Parent=Msa0857170-mRNA-1
chr5_4 exon 52495632 52495795 52495632 ID=Msa0857170-mRNA-1:exon:16389;Parent=Msa0857170-mRNA-1
chr5_4 exon 52497281 52497836 52497281 ID=Msa0857170-mRNA-1:exon:16390;Parent=Msa0857170-mRNA-1
chr5_4 five_prime_UTR 52493840 52494004 52493840 ID=Msa0857170-mRNA-1:five_prime_utr;Parent=Msa0857170-mRNA-1
chr5_4 CDS 52494005 52494412 52494005 ID=Msa0857170-mRNA-1:cds;Parent=Msa0857170-mRNA-1
chr5_4 CDS 52495632 52495795 52495632 ID=Msa0857170-mRNA-1:cds;Parent=Msa0857170-mRNA-1
chr5_4 CDS 52497281 52497368 52497281 ID=Msa0857170-mRNA-1:cds;Parent=Msa0857170-mRNA-1
chr5_4 three_prime_UTR 52497369 52497836 52497369 ID=Msa0857170-mRNA-1:three_prime_utr;Parent=Msa0857170-mRNA-1
Gene Sequence

>Msa0857170

ATGGGTATACTCTACGCGCTGGTGGCGCGTGGAACGGTGGTTCTAGCAGAGTTCACCGGAACAACAACAAACGCCGCCGCAATCGCGCGGCAAATCCTCGAGAAAATCCCCGGAAACAACGATACACACGTATCGTATTCACAAGATCGGTATATTTTTCACGTGAAACGAACCGATGGACTCACGGTGCTGTGTATGGCGGATGATAGCGTTGGACGGAGAATTCCGTTTGCGTTTCTTGAAGAGATTCATCAGAGGTTTGTAAGGAGTTATGGACGTGCGGTTTTGACTGCGGAAGCATATGCTATGAATGATGAATTTTCTAGGGTTTTGAATCAGCAGATGGAGTATTTCTCCAGTGATCCGAATGCTGATAGGATTAATCGGCTTAAAGGTGAAATGTCTCAGGTAAGAAATGTTATGATAGAAAATATTGATAAAGTTTTGGATAGAGGTGATAGATTGGAATTGCTGGTGGATAAAGCTGCTAATATGCAAGGAAATACTTTTCGCTTCAAAAAGCAAGCTCGTCGCTTTAGAAGCACTGTCTGGTGGAGGAATGTCAAGCTCACGATTGCACTTATAGTCATCCTACTTGTGATAGCATATGTTGTGTTGGCTTTTGTTTGCCATGGGCCTGCACTACCGTCTTGCTTCTAG
Protein sequence

>Msa0857170

MGILYALVARGTVVLAEFTGTTTNAAAIARQILEKIPGNNDTHVSYSQDRYIFHVKRTDGLTVLCMADDSVGRRIPFAFLEEIHQRFVRSYGRAVLTAEAYAMNDEFSRVLNQQMEYFSSDPNADRINRLKGEMSQVRNVMIENIDKVLDRGDRLELLVDKAANMQGNTFRFKKQARRFRSTVWWRNVKLTIALIVILLVIAYVVLAFVCHGPALPSCF*