AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0862000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0862000 MtrunA17_Chr5g0442451 97.758 223 4 1 1 223 1 222 2.78e-157 434
Msa0862000 MtrunA17_Chr3g0095581 79.204 226 42 1 3 223 5 230 8.78e-129 362
Msa0862000 MtrunA17_Chr1g0204711 59.016 61 17 2 3 58 44 101 9.08e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0862000 AT2G36900.1 60.455 220 85 1 4 223 8 225 1.98e-92 270
Msa0862000 AT5G50440.1 57.919 221 88 1 3 223 4 219 4.79e-87 256
Msa0862000 AT5G50440.2 57.919 221 88 1 3 223 4 219 4.79e-87 256
Msa0862000 AT2G36900.3 60.377 212 82 1 4 215 8 217 1.14e-85 253
Msa0862000 AT2G36900.2 61.576 203 76 1 4 206 8 208 8.75e-84 248

Find 75 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCGACACCCTAGATTTC+TGG 0.167373 5_4:+61517750 Msa0862000:three_prime_UTR
TGTTCGGAGAAGAAGCTTTC+TGG 0.233566 5_4:-61515023 None:intergenic
TAAAGAAAGAGCAGAGCTTC+TGG 0.253589 5_4:+61516453 Msa0862000:CDS
TCGCAATTCTGCACGTGAAT+TGG 0.274320 5_4:+61516633 Msa0862000:CDS
CCAGAGATCGCGTTGAGATT+TGG 0.282150 5_4:-61515191 None:intergenic
CGAATCGTTAAGAGAGAGTT+TGG 0.318595 5_4:+61516390 Msa0862000:CDS
AACGAAACAGAGTTCAGTTT+TGG 0.325806 5_4:-61514942 None:intergenic
CTTGTTGATTTGATAATATC+TGG 0.329918 5_4:+61517860 Msa0862000:three_prime_UTR
ACAATTCCGCCCAGAAATCT+AGG 0.335764 5_4:-61517760 None:intergenic
CCAAATCTCAACGCGATCTC+TGG 0.360106 5_4:+61515191 Msa0862000:CDS
GGGAACAAAACGTGAGAATA+TGG 0.360893 5_4:-61514909 None:intergenic
AATCAACTCTCGCGATCAAT+TGG 0.373716 5_4:+61515166 Msa0862000:CDS
TCATTTCGACGCATAGAGAT+TGG 0.377146 5_4:-61515144 None:intergenic
ACAATACTTTCATCTATTCA+TGG 0.382319 5_4:+61516679 Msa0862000:CDS
CACCGACACCCTAGATTTCT+GGG 0.384074 5_4:+61517751 Msa0862000:three_prime_UTR
GATAAATCACTAAGCTACTT+TGG 0.388001 5_4:-61518014 None:intergenic
GTTCGAGTCCGTCGCGTGTT+CGG 0.400245 5_4:-61515039 None:intergenic
CCTCCTTCCATGGAGGTTAT+CGG 0.409912 5_4:-61514968 None:intergenic
CAGATTATGTATGTCTTTGA+AGG 0.410349 5_4:-61517708 None:intergenic
GAAGAAGCTTTCTGGCGGTT+TGG 0.418799 5_4:-61515015 None:intergenic
GATTTAAGGTCTATCAAGGC+TGG 0.442049 5_4:+61517649 Msa0862000:three_prime_UTR
TTCATCTATTCATGGACAAA+GGG 0.449639 5_4:+61516687 Msa0862000:CDS
AAAGAAAGAGCAGAGCTTCT+GGG 0.453337 5_4:+61516454 Msa0862000:CDS
GGAGGAGGAAGAAGATGAGT+TGG 0.459191 5_4:-61515083 None:intergenic
CAAACTCTCTCTTAACGATT+CGG 0.459459 5_4:-61516389 None:intergenic
CGTTCCACCGCCTCCTTCCA+TGG 0.462353 5_4:-61514978 None:intergenic
AAGGAATCATAAACGAATGA+TGG 0.468381 5_4:+61516426 Msa0862000:CDS
TGGACAAAGGGAACGCTTGA+AGG 0.477190 5_4:+61516699 Msa0862000:CDS
ACCTCCATGGAAGGAGGCGG+TGG 0.487164 5_4:+61514974 Msa0862000:exon
GACGTAACCGTGTTGATCAA+TGG 0.491291 5_4:+61517497 Msa0862000:CDS
CTCAACGCGATCTCTGGAAA+AGG 0.491381 5_4:+61515197 Msa0862000:CDS
GCATCAACGATTTCTTGCAT+TGG 0.500872 5_4:+61517962 Msa0862000:three_prime_UTR
TGGGCGGAATTGTCAATGAA+TGG 0.501434 5_4:+61517770 Msa0862000:three_prime_UTR
AAGATGAGTTGGAATATTCA+AGG 0.504949 5_4:-61515072 None:intergenic
TTGGATGTTCTCAATACAGC+GGG 0.513191 5_4:+61517442 Msa0862000:CDS
TTTCATCTATTCATGGACAA+AGG 0.521012 5_4:+61516686 Msa0862000:CDS
GCTAACGGCGAAGGAGAGAT+CGG 0.523265 5_4:-61515104 None:intergenic
TTAGTATAATTGAAGACTAG+AGG 0.529829 5_4:-61518072 None:intergenic
AGTTTGTTCAGATTCGTGAC+AGG 0.541998 5_4:+61517801 Msa0862000:three_prime_UTR
GATCAATGGATCAAATATGC+GGG 0.542412 5_4:+61517511 Msa0862000:CDS
AGGAGGCGGTGGAACGCTAT+CGG 0.543763 5_4:+61514985 Msa0862000:CDS
CTGAGTAATGTCTTTGCTAA+CGG 0.551034 5_4:-61515119 None:intergenic
AATGTCTTTGCTAACGGCGA+AGG 0.556807 5_4:-61515113 None:intergenic
TTGGAAAATGCTAATGCACT+TGG 0.557309 5_4:+61516652 Msa0862000:CDS
CAAGGCTGGACCAGCAGAGT+AGG 0.558029 5_4:+61517663 Msa0862000:three_prime_UTR
CGCCCAGAAATCTAGGGTGT+CGG 0.558180 5_4:-61517753 None:intergenic
CGTTGCTCCGATAACCTCCA+TGG 0.562884 5_4:+61514961 Msa0862000:exon
TAGGGTGTCGGTGATAAACA+TGG 0.567746 5_4:-61517741 None:intergenic
GGCGAAGGAGAGATCGGAGG+AGG 0.576045 5_4:-61515098 None:intergenic
ATTGTTGCAATCAATTACAC+TGG 0.579992 5_4:+61518040 Msa0862000:three_prime_UTR
TTGGCATGCTTTGCAATCGA+GGG 0.582855 5_4:+61517981 Msa0862000:three_prime_UTR
TAGTATAATTGAAGACTAGA+GGG 0.587407 5_4:-61518071 None:intergenic
GTTTGGAGAAATATAACTCA+AGG 0.589752 5_4:+61516407 Msa0862000:CDS
CAATTCCGCCCAGAAATCTA+GGG 0.594420 5_4:-61517759 None:intergenic
ACATTTCTCAAGTTCCTCTG+TGG 0.595784 5_4:+61517609 Msa0862000:three_prime_UTR
AATATTCTCAAAACATGACT+AGG 0.597046 5_4:-61516580 None:intergenic
ATTGGCATGCTTTGCAATCG+AGG 0.600103 5_4:+61517980 Msa0862000:three_prime_UTR
GCTCCGATAACCTCCATGGA+AGG 0.605506 5_4:+61514965 Msa0862000:exon
GAGTGCGCATAGGAAAGCAT+TGG 0.620492 5_4:+61517423 Msa0862000:intron
CCGATAACCTCCATGGAAGG+AGG 0.624833 5_4:+61514968 Msa0862000:exon
CTTCTTCTCCGAACACGCGA+CGG 0.630301 5_4:+61515031 Msa0862000:CDS
TCTCAATTAGCCTCAAAACA+CGG 0.632637 5_4:-61517474 None:intergenic
ATAACCTCCATGGAAGGAGG+CGG 0.636247 5_4:+61514971 Msa0862000:exon
TCTTTGTGCAGAGTGCGCAT+AGG 0.655474 5_4:+61517413 Msa0862000:intron
TGATCAATGGATCAAATATG+CGG 0.660838 5_4:+61517510 Msa0862000:CDS
ATCAATGGATCAAATATGCG+GGG 0.662317 5_4:+61517512 Msa0862000:CDS
ATTTGATCCATTGATCAACA+CGG 0.672954 5_4:-61517504 None:intergenic
AGGGTGTCGGTGATAAACAT+GGG 0.673587 5_4:-61517740 None:intergenic
TCCACCGCCTCCTTCCATGG+AGG 0.681217 5_4:-61514975 None:intergenic
TGATACGCAAGCGATGCTGT+CGG 0.681369 5_4:+61516609 Msa0862000:CDS
TCGGAGAAGAAGCTTTCTGG+CGG 0.686541 5_4:-61515020 None:intergenic
AACGGCGAAGGAGAGATCGG+AGG 0.707495 5_4:-61515101 None:intergenic
ATTGGATGTTCTCAATACAG+CGG 0.713965 5_4:+61517441 Msa0862000:CDS
CGACACCCTAGATTTCTGGG+CGG 0.771483 5_4:+61517754 Msa0862000:three_prime_UTR
TGGATGTTCTCAATACAGCG+GGG 0.796958 5_4:+61517443 Msa0862000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTCTCAAATTATTAT+TGG + chr5_4:61516120-61516139 Msa0862000:intron 10.0%
!!! TTATGTTTTGTTAAATTTCA+AGG + chr5_4:61515915-61515934 Msa0862000:intron 15.0%
!! ATTTCTTAAATTATTACTGA+TGG + chr5_4:61516228-61516247 Msa0862000:intron 15.0%
!!! ATGATTTTTGTTGTTAATTA+TGG + chr5_4:61516540-61516559 Msa0862000:intron 15.0%
!!! TATTTCATGTTTGTTATTTT+TGG + chr5_4:61516867-61516886 Msa0862000:intron 15.0%
!!! ATTTCATGTTTGTTATTTTT+GGG + chr5_4:61516868-61516887 Msa0862000:intron 15.0%
!!! GTGTATAAAATTTTGATTTA+AGG + chr5_4:61517635-61517654 Msa0862000:three_prime_UTR 15.0%
!!! GTATCGATTATTAATCTTAA+CGG + chr5_4:61515264-61515283 Msa0862000:intron 20.0%
!! TAATCTGTAATTTCAATTCT+CGG + chr5_4:61515957-61515976 Msa0862000:intron 20.0%
!!! TTTCATGTTTGTTATTTTTG+GGG + chr5_4:61516869-61516888 Msa0862000:intron 20.0%
!!! AATTACAATATACATTTTGC+TGG - chr5_4:61516922-61516941 None:intergenic 20.0%
!! AATTATTCCTAGTTGAAATT+TGG - chr5_4:61517148-61517167 None:intergenic 20.0%
!!! TGAATTTAATCATTTTAGCA+AGG - chr5_4:61517838-61517857 None:intergenic 20.0%
!!! CAAGTGTCAAAATTTTTTTT+TGG + chr5_4:61518099-61518118 Msa0862000:three_prime_UTR 20.0%
!!! TCAAGTTTTGTTAAATGTCA+AGG + chr5_4:61515394-61515413 Msa0862000:intron 25.0%
!! ATCCTGTCTAAGATTATTAT+TGG + chr5_4:61515581-61515600 Msa0862000:intron 25.0%
! TACCAATAATAATCTTAGAC+AGG - chr5_4:61515586-61515605 None:intergenic 25.0%
!! TATTCTAGTTATTAGGATCT+TGG + chr5_4:61515823-61515842 Msa0862000:intron 25.0%
!! TGTTCTAATTGTGATGTTTT+TGG + chr5_4:61516335-61516354 Msa0862000:intron 25.0%
! TTAATTATGGTGAAACAGAA+CGG + chr5_4:61516553-61516572 Msa0862000:intron 25.0%
! AATATTCTCAAAACATGACT+AGG - chr5_4:61516583-61516602 None:intergenic 25.0%
! ACAATACTTTCATCTATTCA+TGG + chr5_4:61516679-61516698 Msa0862000:CDS 25.0%
!! AATAGCTTGAATTGATGATT+AGG + chr5_4:61516767-61516786 Msa0862000:intron 25.0%
!!! TTCATGTTTGTTATTTTTGG+GGG + chr5_4:61516870-61516889 Msa0862000:intron 25.0%
! ATAAGTAGCTATTCTATTGT+TGG + chr5_4:61516943-61516962 Msa0862000:intron 25.0%
!! AAACATGTAAAGAGGATTTT+TGG + chr5_4:61517040-61517059 Msa0862000:intron 25.0%
! TGTTAACATGAATGCTAATA+TGG - chr5_4:61517330-61517349 None:intergenic 25.0%
! AGATATAAACCAAATATGAC+AGG - chr5_4:61517396-61517415 None:intergenic 25.0%
!!! ATGTCAATTTTTCAACAACT+GGG - chr5_4:61517593-61517612 None:intergenic 25.0%
!!! AATGTCAATTTTTCAACAAC+TGG - chr5_4:61517594-61517613 None:intergenic 25.0%
!!! TTTTGATTTAAGGTCTATCA+AGG + chr5_4:61517645-61517664 Msa0862000:three_prime_UTR 25.0%
!!! CTTGTTGATTTGATAATATC+TGG + chr5_4:61517860-61517879 Msa0862000:three_prime_UTR 25.0%
! TAGTATAATTGAAGACTAGA+GGG - chr5_4:61518074-61518093 None:intergenic 25.0%
! TTAGTATAATTGAAGACTAG+AGG - chr5_4:61518075-61518094 None:intergenic 25.0%
! AAGATGAGTTGGAATATTCA+AGG - chr5_4:61515075-61515094 None:intergenic 30.0%
! CAGATTTTCAATGTTCACTA+CGG - chr5_4:61515461-61515480 None:intergenic 30.0%
TTCGAACTGACAATTTAGAT+TGG + chr5_4:61515639-61515658 Msa0862000:intron 30.0%
GTTCTAATTATGATGCCTTT+AGG + chr5_4:61515752-61515771 Msa0862000:intron 30.0%
! ATCCACGTATTCTAGTTATT+AGG + chr5_4:61515816-61515835 Msa0862000:intron 30.0%
ATCCTAATAACTAGAATACG+TGG - chr5_4:61515821-61515840 None:intergenic 30.0%
CATAGAGTCAATTGAAACAA+CGG - chr5_4:61515874-61515893 None:intergenic 30.0%
! TGCAGTTACTATTCTTTTCT+AGG + chr5_4:61516006-61516025 Msa0862000:intron 30.0%
!!! AATTGTGATGTTTTTGGTGT+AGG + chr5_4:61516341-61516360 Msa0862000:intron 30.0%
GTTTGGAGAAATATAACTCA+AGG + chr5_4:61516407-61516426 Msa0862000:CDS 30.0%
AAGGAATCATAAACGAATGA+TGG + chr5_4:61516426-61516445 Msa0862000:CDS 30.0%
TAATTATGGTGAAACAGAAC+GGG + chr5_4:61516554-61516573 Msa0862000:intron 30.0%
TTTCATCTATTCATGGACAA+AGG + chr5_4:61516686-61516705 Msa0862000:CDS 30.0%
TTCATCTATTCATGGACAAA+GGG + chr5_4:61516687-61516706 Msa0862000:CDS 30.0%
AAACCTAAACGATAGCATAA+TGG - chr5_4:61516806-61516825 None:intergenic 30.0%
!!! TTTGTTATTTTTGGGGGTTA+TGG + chr5_4:61516876-61516895 Msa0862000:intron 30.0%
!!! TTGTTATTTTTGGGGGTTAT+GGG + chr5_4:61516877-61516896 Msa0862000:intron 30.0%
! CAATATACATTTTGCTGGAA+AGG - chr5_4:61516917-61516936 None:intergenic 30.0%
TAAAGTGAATGTGAGACATA+AGG - chr5_4:61516980-61516999 None:intergenic 30.0%
GACAACTTAAACATGTAAAG+AGG + chr5_4:61517032-61517051 Msa0862000:intron 30.0%
! CTAGGAATAATTTTTGTCGA+TGG + chr5_4:61517156-61517175 Msa0862000:intron 30.0%
! TCTGCAGATTTGAGATTATA+TGG - chr5_4:61517197-61517216 None:intergenic 30.0%
TAACATCTAAGAATTGAGCA+GGG - chr5_4:61517262-61517281 None:intergenic 30.0%
ATAACATCTAAGAATTGAGC+AGG - chr5_4:61517263-61517282 None:intergenic 30.0%
!!! TGATCTACTAGTGTTACTTT+TGG + chr5_4:61517354-61517373 Msa0862000:intron 30.0%
ACTAACATACCTGTCATATT+TGG + chr5_4:61517384-61517403 Msa0862000:intron 30.0%
ATTTGATCCATTGATCAACA+CGG - chr5_4:61517507-61517526 None:intergenic 30.0%
TGATCAATGGATCAAATATG+CGG + chr5_4:61517510-61517529 Msa0862000:CDS 30.0%
!! TCTTGTTTGCTTTTGTTCTA+TGG + chr5_4:61517554-61517573 Msa0862000:CDS 30.0%
! ATTTTATACACTTTCCACAG+AGG - chr5_4:61517626-61517645 None:intergenic 30.0%
CAGATTATGTATGTCTTTGA+AGG - chr5_4:61517711-61517730 None:intergenic 30.0%
!! TTTAATCATTTTAGCAAGGC+TGG - chr5_4:61517834-61517853 None:intergenic 30.0%
GATAAATCACTAAGCTACTT+TGG - chr5_4:61518017-61518036 None:intergenic 30.0%
ATTGTTGCAATCAATTACAC+TGG + chr5_4:61518040-61518059 Msa0862000:three_prime_UTR 30.0%
!!! TTCAGTTTTGGAAACGAAAC+GGG - chr5_4:61514933-61514952 None:intergenic 35.0%
!!! GTTCAGTTTTGGAAACGAAA+CGG - chr5_4:61514934-61514953 None:intergenic 35.0%
! AACGAAACAGAGTTCAGTTT+TGG - chr5_4:61514945-61514964 None:intergenic 35.0%
CTGAGTAATGTCTTTGCTAA+CGG - chr5_4:61515122-61515141 None:intergenic 35.0%
GTAATCACATATGTACGTGT+CGG - chr5_4:61515556-61515575 None:intergenic 35.0%
GAACTGACAATTTAGATTGG+AGG + chr5_4:61515642-61515661 Msa0862000:intron 35.0%
! TGTTCTAGTTGTTAGGATCT+TGG + chr5_4:61515728-61515747 Msa0862000:intron 35.0%
TCAATTCTCGGTAGTGAAAT+TGG + chr5_4:61515969-61515988 Msa0862000:intron 35.0%
CAATTCTCGGTAGTGAAATT+GGG + chr5_4:61515970-61515989 Msa0862000:intron 35.0%
TTCATACCGACACTTTAGAT+TGG + chr5_4:61516171-61516190 Msa0862000:intron 35.0%
! TGCATGTTGTTCTAGTTGTT+AGG + chr5_4:61516305-61516324 Msa0862000:intron 35.0%
!!! ATGTTTTTGGTGTAGGAAAG+TGG + chr5_4:61516348-61516367 Msa0862000:intron 35.0%
CAAACTCTCTCTTAACGATT+CGG - chr5_4:61516392-61516411 None:intergenic 35.0%
AATTATGGTGAAACAGAACG+GGG + chr5_4:61516555-61516574 Msa0862000:intron 35.0%
TTGGAAAATGCTAATGCACT+TGG + chr5_4:61516652-61516671 Msa0862000:CDS 35.0%
! AGCTTGAATTGATGATTAGG+AGG + chr5_4:61516770-61516789 Msa0862000:intron 35.0%
! GCTTGAATTGATGATTAGGA+GGG + chr5_4:61516771-61516790 Msa0862000:intron 35.0%
TCACCATTATGCTATCGTTT+AGG + chr5_4:61516800-61516819 Msa0862000:intron 35.0%
!!! TTTACCTTGTTTTGTTGCTC+TGG + chr5_4:61516996-61517015 Msa0862000:intron 35.0%
TGCAGTTCCAAATTTCAACT+AGG + chr5_4:61517138-61517157 Msa0862000:intron 35.0%
AACATCTAAGAATTGAGCAG+GGG - chr5_4:61517261-61517280 None:intergenic 35.0%
ATGTTCTTCATCAGCTTAGT+TGG + chr5_4:61517281-61517300 Msa0862000:intron 35.0%
!! TTCATCAGCTTAGTTGGTTT+TGG + chr5_4:61517287-61517306 Msa0862000:intron 35.0%
ATTGGATGTTCTCAATACAG+CGG + chr5_4:61517441-61517460 Msa0862000:CDS 35.0%
! TCTCAATTAGCCTCAAAACA+CGG - chr5_4:61517477-61517496 None:intergenic 35.0%
GATCAATGGATCAAATATGC+GGG + chr5_4:61517511-61517530 Msa0862000:CDS 35.0%
ATCAATGGATCAAATATGCG+GGG + chr5_4:61517512-61517531 Msa0862000:CDS 35.0%
!! GTTTTCTTTTACAAGTGCTG+AGG + chr5_4:61517928-61517947 Msa0862000:three_prime_UTR 35.0%
GGGAACAAAACGTGAGAATA+TGG - chr5_4:61514912-61514931 None:intergenic 40.0%
!!! TCAGTTTTGGAAACGAAACG+GGG - chr5_4:61514932-61514951 None:intergenic 40.0%
TCATTTCGACGCATAGAGAT+TGG - chr5_4:61515147-61515166 None:intergenic 40.0%
AATCAACTCTCGCGATCAAT+TGG + chr5_4:61515166-61515185 Msa0862000:CDS 40.0%
TCGAAGTACAGACATAGACA+TGG - chr5_4:61515624-61515643 None:intergenic 40.0%
! CACGTGTTGTTCTAGTTGTT+AGG + chr5_4:61515721-61515740 Msa0862000:intron 40.0%
TCGGTATGAAGCACAGATAT+CGG - chr5_4:61516161-61516180 None:intergenic 40.0%
ATACCGACACTTTAGATTGG+AGG + chr5_4:61516174-61516193 Msa0862000:intron 40.0%
! ACACCTCCAATCTAAAGTGT+CGG - chr5_4:61516180-61516199 None:intergenic 40.0%
! CTTTAGATTGGAGGTGTGTT+TGG + chr5_4:61516183-61516202 Msa0862000:intron 40.0%
CGAATCGTTAAGAGAGAGTT+TGG + chr5_4:61516390-61516409 Msa0862000:CDS 40.0%
TAAAGAAAGAGCAGAGCTTC+TGG + chr5_4:61516453-61516472 Msa0862000:CDS 40.0%
AAAGAAAGAGCAGAGCTTCT+GGG + chr5_4:61516454-61516473 Msa0862000:CDS 40.0%
TCCTATTTCCTGCTTTCACT+CGG + chr5_4:61516727-61516746 Msa0862000:intron 40.0%
!!! AGAGGATTTTTGGAACTGCA+CGG + chr5_4:61517050-61517069 Msa0862000:intron 40.0%
GTGATTTCAAAGCTTGGGTT+AGG - chr5_4:61517096-61517115 None:intergenic 40.0%
! TTTGAAATCACCACCCTTTG+AGG + chr5_4:61517105-61517124 Msa0862000:intron 40.0%
TTGAAATCACCACCCTTTGA+GGG + chr5_4:61517106-61517125 Msa0862000:intron 40.0%
ACATCATGCATGTTCCATGA+GGG - chr5_4:61517221-61517240 None:intergenic 40.0%
TTGGATGTTCTCAATACAGC+GGG + chr5_4:61517442-61517461 Msa0862000:CDS 40.0%
! GGATATCAAACCGTGTTTTG+AGG + chr5_4:61517464-61517483 Msa0862000:CDS 40.0%
ACATTTCTCAAGTTCCTCTG+TGG + chr5_4:61517609-61517628 Msa0862000:three_prime_UTR 40.0%
! GATTTAAGGTCTATCAAGGC+TGG + chr5_4:61517649-61517668 Msa0862000:three_prime_UTR 40.0%
! TTCTGAAAAACCTACTCTGC+TGG - chr5_4:61517676-61517695 None:intergenic 40.0%
! AGTTTGTTCAGATTCGTGAC+AGG + chr5_4:61517801-61517820 Msa0862000:three_prime_UTR 40.0%
!!! TCTTTTACAAGTGCTGAGGA+TGG + chr5_4:61517932-61517951 Msa0862000:three_prime_UTR 40.0%
GCATCAACGATTTCTTGCAT+TGG + chr5_4:61517962-61517981 Msa0862000:three_prime_UTR 40.0%
TGTTCGGAGAAGAAGCTTTC+TGG - chr5_4:61515026-61515045 None:intergenic 45.0%
AATGTCTTTGCTAACGGCGA+AGG - chr5_4:61515116-61515135 None:intergenic 45.0%
ACATCTTCGAGTAGACGTGT+CGG - chr5_4:61515493-61515512 None:intergenic 45.0%
ACGTCTACTCGAAGATGTGT+CGG + chr5_4:61515495-61515514 Msa0862000:intron 45.0%
CACGTTCAATAACACACGTG+CGG - chr5_4:61515521-61515540 None:intergenic 45.0%
GACACTACACTGACACCTAA+AGG - chr5_4:61515770-61515789 None:intergenic 45.0%
GGTAGATATAGCACCATCGA+AGG + chr5_4:61516027-61516046 Msa0862000:intron 45.0%
ATATAGCACCATCGAAGGTG+TGG + chr5_4:61516032-61516051 Msa0862000:intron 45.0%
TCGCAATTCTGCACGTGAAT+TGG + chr5_4:61516633-61516652 Msa0862000:CDS 45.0%
CGTCTCGTTTCTATTGTGAC+TGG + chr5_4:61516837-61516856 Msa0862000:intron 45.0%
CTCGTTTCTATTGTGACTGG+TGG + chr5_4:61516840-61516859 Msa0862000:intron 45.0%
GAAGCCAGAGCAACAAAACA+AGG - chr5_4:61517003-61517022 None:intergenic 45.0%
! CTTGGGTTAGGAGCAAGAAA+TGG - chr5_4:61517084-61517103 None:intergenic 45.0%
GGGTGGTGATTTCAAAGCTT+GGG - chr5_4:61517101-61517120 None:intergenic 45.0%
AGGGTGGTGATTTCAAAGCT+TGG - chr5_4:61517102-61517121 None:intergenic 45.0%
TGAAATCACCACCCTTTGAG+GGG + chr5_4:61517107-61517126 Msa0862000:intron 45.0%
TCAAATCTGCAGATCCCTCA+TGG + chr5_4:61517204-61517223 Msa0862000:intron 45.0%
GACATCATGCATGTTCCATG+AGG - chr5_4:61517222-61517241 None:intergenic 45.0%
ATCTAAGAATTGAGCAGGGG+CGG - chr5_4:61517258-61517277 None:intergenic 45.0%
TGGATGTTCTCAATACAGCG+GGG + chr5_4:61517443-61517462 Msa0862000:CDS 45.0%
! GACGTAACCGTGTTGATCAA+TGG + chr5_4:61517497-61517516 Msa0862000:CDS 45.0%
!! AGGGTGTCGGTGATAAACAT+GGG - chr5_4:61517743-61517762 None:intergenic 45.0%
!! TAGGGTGTCGGTGATAAACA+TGG - chr5_4:61517744-61517763 None:intergenic 45.0%
CAATTCCGCCCAGAAATCTA+GGG - chr5_4:61517762-61517781 None:intergenic 45.0%
ACAATTCCGCCCAGAAATCT+AGG - chr5_4:61517763-61517782 None:intergenic 45.0%
TGGGCGGAATTGTCAATGAA+TGG + chr5_4:61517770-61517789 Msa0862000:three_prime_UTR 45.0%
ATTGGCATGCTTTGCAATCG+AGG + chr5_4:61517980-61517999 Msa0862000:three_prime_UTR 45.0%
TTGGCATGCTTTGCAATCGA+GGG + chr5_4:61517981-61518000 Msa0862000:three_prime_UTR 45.0%
CCTCCTTCCATGGAGGTTAT+CGG - chr5_4:61514971-61514990 None:intergenic 50.0%
ATAACCTCCATGGAAGGAGG+CGG + chr5_4:61514971-61514990 Msa0862000:exon 50.0%
! GAAGAAGCTTTCTGGCGGTT+TGG - chr5_4:61515018-61515037 None:intergenic 50.0%
TCGGAGAAGAAGCTTTCTGG+CGG - chr5_4:61515023-61515042 None:intergenic 50.0%
GGAGGAGGAAGAAGATGAGT+TGG - chr5_4:61515086-61515105 None:intergenic 50.0%
CCAGAGATCGCGTTGAGATT+TGG - chr5_4:61515194-61515213 None:intergenic 50.0%
CCAAATCTCAACGCGATCTC+TGG + chr5_4:61515191-61515210 Msa0862000:CDS 50.0%
! CTCAACGCGATCTCTGGAAA+AGG + chr5_4:61515197-61515216 Msa0862000:CDS 50.0%
ATACACCACCACACCTTCGA+TGG - chr5_4:61516043-61516062 None:intergenic 50.0%
TGATACGCAAGCGATGCTGT+CGG + chr5_4:61516609-61516628 Msa0862000:CDS 50.0%
TGGACAAAGGGAACGCTTGA+AGG + chr5_4:61516699-61516718 Msa0862000:CDS 50.0%
GCCGAGTGAAAGCAGGAAAT+AGG - chr5_4:61516731-61516750 None:intergenic 50.0%
CTTTGTTGCCGAGTGAAAGC+AGG - chr5_4:61516738-61516757 None:intergenic 50.0%
GGGTTATGGGTGAACTGAAG+TGG + chr5_4:61516890-61516909 Msa0862000:intron 50.0%
TCTAAGAATTGAGCAGGGGC+GGG - chr5_4:61517257-61517276 None:intergenic 50.0%
TCTTTGTGCAGAGTGCGCAT+AGG + chr5_4:61517413-61517432 Msa0862000:intron 50.0%
! GAGTGCGCATAGGAAAGCAT+TGG + chr5_4:61517423-61517442 Msa0862000:intron 50.0%
TCACCGACACCCTAGATTTC+TGG + chr5_4:61517750-61517769 Msa0862000:three_prime_UTR 50.0%
CACCGACACCCTAGATTTCT+GGG + chr5_4:61517751-61517770 Msa0862000:three_prime_UTR 50.0%
CGTTGCTCCGATAACCTCCA+TGG + chr5_4:61514961-61514980 Msa0862000:exon 55.0%
GCTCCGATAACCTCCATGGA+AGG + chr5_4:61514965-61514984 Msa0862000:exon 55.0%
CCGATAACCTCCATGGAAGG+AGG + chr5_4:61514968-61514987 Msa0862000:exon 55.0%
CTTCTTCTCCGAACACGCGA+CGG + chr5_4:61515031-61515050 Msa0862000:CDS 55.0%
GCTAACGGCGAAGGAGAGAT+CGG - chr5_4:61515107-61515126 None:intergenic 55.0%
! TAGCACCATCGAAGGTGTGG+TGG + chr5_4:61516035-61516054 Msa0862000:intron 55.0%
! AATCACCACCCTTTGAGGGG+TGG + chr5_4:61517110-61517129 Msa0862000:intron 55.0%
GCAGTAGACCACCCCTCAAA+GGG - chr5_4:61517121-61517140 None:intergenic 55.0%
TGCAGTAGACCACCCCTCAA+AGG - chr5_4:61517122-61517141 None:intergenic 55.0%
CTAAGAATTGAGCAGGGGCG+GGG - chr5_4:61517256-61517275 None:intergenic 55.0%
! CGCCCAGAAATCTAGGGTGT+CGG - chr5_4:61517756-61517775 None:intergenic 55.0%
! CGACACCCTAGATTTCTGGG+CGG + chr5_4:61517754-61517773 Msa0862000:three_prime_UTR 55.0%
AGGAGGCGGTGGAACGCTAT+CGG + chr5_4:61514985-61515004 Msa0862000:CDS 60.0%
GTTCGAGTCCGTCGCGTGTT+CGG - chr5_4:61515042-61515061 None:intergenic 60.0%
AACGGCGAAGGAGAGATCGG+AGG - chr5_4:61515104-61515123 None:intergenic 60.0%
GTAGACCACCCCTCAAAGGG+TGG - chr5_4:61517118-61517137 None:intergenic 60.0%
CAAGGCTGGACCAGCAGAGT+AGG + chr5_4:61517663-61517682 Msa0862000:three_prime_UTR 60.0%
ACCTCCATGGAAGGAGGCGG+TGG + chr5_4:61514974-61514993 Msa0862000:exon 65.0%
TCCACCGCCTCCTTCCATGG+AGG - chr5_4:61514978-61514997 None:intergenic 65.0%
CGTTCCACCGCCTCCTTCCA+TGG - chr5_4:61514981-61515000 None:intergenic 65.0%
GGCGAAGGAGAGATCGGAGG+AGG - chr5_4:61515101-61515120 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 61514836 61518141 61514836 ID=Msa0862000;Name=Msa0862000
chr5_4 mRNA 61514836 61518141 61514836 ID=Msa0862000-mRNA-1;Parent=Msa0862000;Name=Msa0862000-mRNA-1;_AED=0.18;_eAED=0.18;_QI=144|1|1|1|1|1|4|556|223
chr5_4 exon 61514836 61515218 61514836 ID=Msa0862000-mRNA-1:exon:19086;Parent=Msa0862000-mRNA-1
chr5_4 exon 61516363 61516483 61516363 ID=Msa0862000-mRNA-1:exon:19087;Parent=Msa0862000-mRNA-1
chr5_4 exon 61516571 61516720 61516571 ID=Msa0862000-mRNA-1:exon:19088;Parent=Msa0862000-mRNA-1
chr5_4 exon 61517424 61518141 61517424 ID=Msa0862000-mRNA-1:exon:19089;Parent=Msa0862000-mRNA-1
chr5_4 five_prime_UTR 61514836 61514979 61514836 ID=Msa0862000-mRNA-1:five_prime_utr;Parent=Msa0862000-mRNA-1
chr5_4 CDS 61514980 61515218 61514980 ID=Msa0862000-mRNA-1:cds;Parent=Msa0862000-mRNA-1
chr5_4 CDS 61516363 61516483 61516363 ID=Msa0862000-mRNA-1:cds;Parent=Msa0862000-mRNA-1
chr5_4 CDS 61516571 61516720 61516571 ID=Msa0862000-mRNA-1:cds;Parent=Msa0862000-mRNA-1
chr5_4 CDS 61517424 61517585 61517424 ID=Msa0862000-mRNA-1:cds;Parent=Msa0862000-mRNA-1
chr5_4 three_prime_UTR 61517586 61518141 61517586 ID=Msa0862000-mRNA-1:three_prime_utr;Parent=Msa0862000-mRNA-1
Gene Sequence

>Msa0862000

ATGGAAGGAGGCGGTGGAACGCTATCGGAGATTCACCAAACCGCCAGAAAGCTTCTTCTCCGAACACGCGACGGACTCGAACGTCTCGAACGCCTTGAATATTCCAACTCATCTTCTTCCTCCTCCGATCTCTCCTTCGCCGTTAGCAAAGACATTACTCAGATCCAATCTCTATGCGTCGAAATGAATCAACTCTCGCGATCAATTGGCGCCAAATCTCAACGCGATCTCTGGAAAAGGAAAGTGGAACAAATAGCTGAAGAAGCCGAATCGTTAAGAGAGAGTTTGGAGAAATATAACTCAAGGAATCATAAACGAATGATGGAAGCTAAAGAAAGAGCAGAGCTTCTGGGAAGAGTTAACGGGGATCCTAGTCATGTTTTGAGAATATTCGACGATGATACGCAAGCGATGCTGTCGGTTCGCAATTCTGCACGTGAATTGGAAAATGCTAATGCACTTGGAGAGACAATACTTTCATCTATTCATGGACAAAGGGAACGCTTGAAGAGTGCGCATAGGAAAGCATTGGATGTTCTCAATACAGCGGGGATATCAAACCGTGTTTTGAGGCTAATTGAGAGACGTAACCGTGTTGATCAATGGATCAAATATGCGGGGATGATTTTGACTGTCATTTTCTTGTTTGCTTTTGTTCTATGGAGACGTTAA
Protein sequence

>Msa0862000

MEGGGGTLSEIHQTARKLLLRTRDGLERLERLEYSNSSSSSSDLSFAVSKDITQIQSLCVEMNQLSRSIGAKSQRDLWKRKVEQIAEEAESLRESLEKYNSRNHKRMMEAKERAELLGRVNGDPSHVLRIFDDDTQAMLSVRNSARELENANALGETILSSIHGQRERLKSAHRKALDVLNTAGISNRVLRLIERRNRVDQWIKYAGMILTVIFLFAFVLWRR*