AlfalfaGEDB | Msa0874610

M. sativa cultivar zhongmu-4 / Msa0874610

KEGG: -
GO: GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958
PFAM: PF12819.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874610	KEH25125.1	87.731	432	45	2	1	424	1	432	0.0	773

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874610	sp\|Q9SCZ4\|FERON_ARATH	40.330	424	217	16	18	426	21	423	1.01e-75	256

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874610	A0A072UH49	87.731	432	45	2	1	424	1	432	0.0	773

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0342060	Msa0874610	0.938856	3.361763e-99	-8.615850e-47
Msa0874610	Msa1448840	0.804050	2.601286e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874610	MtrunA17_Chr6g0455311	87.731	432	45	2	1	424	1	432	0.0	768
Msa0874610	MtrunA17_Chr6g0455321	84.719	445	49	6	1	433	1	438	0.0	747
Msa0874610	MtrunA17_Chr5g0420171	38.427	445	240	14	1	428	2	429	1.39e-83	273
Msa0874610	MtrunA17_Chr7g0219781	39.636	439	231	19	26	438	31	461	7.17e-78	259
Msa0874610	MtrunA17_Chr7g0219671	40.380	421	222	17	26	424	29	442	3.82e-75	251
Msa0874610	MtrunA17_Chr7g0219601	39.467	413	215	17	26	413	29	431	7.16e-74	248
Msa0874610	MtrunA17_Chr5g0420151	36.343	443	253	12	1	428	2	430	1.53e-73	246
Msa0874610	MtrunA17_Chr7g0245131	37.875	433	237	13	11	428	10	425	2.38e-73	247
Msa0874610	MtrunA17_Chr4g0025961	38.652	445	231	18	7	422	5	436	1.16e-72	245
Msa0874610	MtrunA17_Chr7g0219591	40.559	429	206	19	26	425	29	437	1.71e-72	238

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0874610	AT3G51550.1	40.330	424	217	16	18	426	21	423	1.02e-76	256
Msa0874610	AT5G39000.1	37.379	412	228	15	27	426	26	419	3.67e-68	233
Msa0874610	AT5G38990.1	36.552	435	248	17	4	427	1	418	5.40e-63	219
Msa0874610	AT5G39020.1	36.010	411	233	15	26	425	22	413	1.94e-62	216
Msa0874610	AT5G39030.1	34.515	423	244	15	11	420	5	407	7.10e-57	201
Msa0874610	AT5G39030.2	35.135	407	231	15	27	420	15	401	2.66e-56	199
Msa0874610	AT5G28680.1	32.952	437	257	17	10	433	7	420	1.67e-52	189
Msa0874610	AT5G28680.2	32.952	437	257	17	10	433	7	420	1.67e-52	189
Msa0874610	AT3G04690.1	33.907	407	227	18	35	426	31	410	1.24e-51	186
Msa0874610	AT1G30570.1	27.465	426	260	19	7	412	9	405	3.46e-34	136

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Find 100 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGGTCAATTGGTTCGT+AGG	0.516426	6_1:+10402590	None:intergenic
AAAATGCTTAAAGAGTTTCC+CGG	0.508755	6_1:+10400195	None:intergenic
AAAATTCAAATGCATGCTTA+TGG	0.358421	6_1:-10400608	Msa0874610:CDS
AAACCGTTGGTATAAGTAGT+AGG	0.406261	6_1:+10402339	None:intergenic
AAAGAATATGTTATTAACGT+TGG	0.468220	6_1:-10402210	Msa0874610:CDS
AAATGCTTAAAGAGTTTCCC+GGG	0.557329	6_1:+10400196	None:intergenic
AAATGGACACAAAATCAAAA+AGG	0.416019	6_1:-10400704	Msa0874610:CDS
AAATGGTTTCACACTTCTTA+AGG	0.289321	6_1:-10402283	Msa0874610:CDS
AACCGTTGGTATAAGTAGTA+GGG	0.547927	6_1:+10402340	None:intergenic
AACCTCAATTGTGGCACAAC+AGG	0.373259	6_1:-10402567	Msa0874610:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATGATGATAAGGTTTCTT+TGG	+	chr6_1:10400244-10400263	None:intergenic	30.0%
ATGTGATGAAGATGATGATA+AGG	+	chr6_1:10400253-10400272	None:intergenic	30.0%
AAATGGTTTCACACTTCTTA+AGG	-	chr6_1:10400734-10400753	Msa0874610:CDS	30.0%
AATAGTATATGCTGGTATGA+TGG	+	chr6_1:10400982-10401001	None:intergenic	30.0%
TATGTGTCTTTATATGCCAT+CGG	-	chr6_1:10401129-10401148	Msa0874610:intron	30.0%
TTTGATTCTGTAATCTGCAA+TGG	+	chr6_1:10401915-10401934	None:intergenic	30.0%
CACATTTCCTTGTAGATAAA+CGG	+	chr6_1:10402269-10402288	None:intergenic	30.0%
GTCTCAACCAATTATGTTAT+TGG	+	chr6_1:10402722-10402741	None:intergenic	30.0%
ACATAATTGGTTGAGACAAA+TGG	-	chr6_1:10402725-10402744	Msa0874610:five_prime_UTR	30.0%
CTTGATCTTAAAATCACTAC+AGG	+	chr6_1:10402752-10402771	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	10400202	10402837	10400202	ID=Msa0874610;Name=Msa0874610
chr6_1	mRNA	10400202	10402837	10400202	ID=Msa0874610-mRNA-1;Parent=Msa0874610;Name=Msa0874610-mRNA-1;_AED=0.19;_eAED=0.19;_QI=149\|0.5\|0.33\|0.66\|1\|1\|3\|0\|439
chr6_1	exon	10400202	10400243	10400202	ID=Msa0874610-mRNA-1:exon:3237;Parent=Msa0874610-mRNA-1
chr6_1	exon	10400517	10401118	10400517	ID=Msa0874610-mRNA-1:exon:3236;Parent=Msa0874610-mRNA-1
chr6_1	exon	10402013	10402837	10402013	ID=Msa0874610-mRNA-1:exon:3235;Parent=Msa0874610-mRNA-1
chr6_1	five_prime_UTR	10402689	10402837	10402689	ID=Msa0874610-mRNA-1:five_prime_utr;Parent=Msa0874610-mRNA-1
chr6_1	CDS	10402013	10402688	10402013	ID=Msa0874610-mRNA-1:cds;Parent=Msa0874610-mRNA-1
chr6_1	CDS	10400517	10401118	10400517	ID=Msa0874610-mRNA-1:cds;Parent=Msa0874610-mRNA-1
chr6_1	CDS	10400202	10400243	10400202	ID=Msa0874610-mRNA-1:cds;Parent=Msa0874610-mRNA-1

Gene Sequence

>Msa0874610

ATGAAACCATTGATAAACTTCAAAACCATAATCATCACCCTCTTATACTTTTCCACCTCCCTCTTACACCTTCAAGCCTACGAACCAATTGACCGTTTTAACCTCAATTGTGGCACAACAGGAAACTCTACCGACGGACAAAGAACATGGACAGGAGACATAAACTCAAAACTTTTGTCATCATTACAAAACAACCCAACAACAGTTTCTGTTCCAAAAACAACACAAAACAAAGTTCCATATTCATCAGCTCGTTTATCACACTCAATATTCAACTATTCTTTACCCGTCAGTACAGGTCCTAAATTCCTCCGTCTGTTTTTCTACCCTACTACTTATACCAACGGTTTTAACCGTTATGATGCTTTTTTTACCGTTATTTCAAATGGTTTCACACTTCTTAAGGATTTTAATGCTTCATTAAACGCTGATGTTGAAGGTGTTGATACTATATTGAAAGAATATGTTATTAACGTTGGTGATGATCAAAGGCTTGATTTGAGTTTTATTCCAAGTAGTGGAAATTCTAATAATTATGGTTTTATTAATGGAATTGAAGTCTTATCCATGCCTGATGATCTATACTATACACCACTTGAGGATCCTGGATTTTCACTTGTGGGGTCCACCATCATACCAGCATATACTATTCCAACCAGTGTTGCACTAGAGACTGATTACAGAATCAAAGTTGGAGTGCAAGAAAACTCACCAAACATTCAAGACACAGGCATGCTCAGAAACTGGAACATCAATGACACAACCTATTTAAGAACACCAACATCACGTGACTTCGTCCTAGCAGACATATCAGGTACGAGGAACATAACTGTAACACCCGATTACATAGCACCAAAAGACCTCTTCAAATCATCACGTGACTTAGGAACCAATGCTACTCTCAACAGACTCTTGAACCTAACATGGGAATTCCCCGTTGATTCCGGCTTCACTTACTTTGTCAGGCTCCATTTCTGTGAAGTCGACCCGAACATAACTAGGGCGGGCGATAGAGTCTTCACCGTTTATCTACAAGGAAATGTGGCTGAAGAACAAGCTGATGTTTTGAAATGGACACAAAATCAAAAAGGTCTTGCAGTGCAACGAAACTACGCAGTTTTGATTCCAAAGAGTGAAAGTAATAATAACAAAAAAGTTAACCTCAAAATTCAAATGCATGCTTATGGTGGTAATGGTGACCCATTTTTAAACGGATTAGAAATTTTCAAAATCAGTGATGTTGTTTCGAAGAATCTTGCTGGAGCGAATCCAGATCCGGAAAAGGGTCTTGTTAACGAGTGTTCCCGGGAAACTCTTTAA

Protein sequence

>Msa0874610

MKPLINFKTIIITLLYFSTSLLHLQAYEPIDRFNLNCGTTGNSTDGQRTWTGDINSKLLSSLQNNPTTVSVPKTTQNKVPYSSARLSHSIFNYSLPVSTGPKFLRLFFYPTTYTNGFNRYDAFFTVISNGFTLLKDFNASLNADVEGVDTILKEYVINVGDDQRLDLSFIPSSGNSNNYGFINGIEVLSMPDDLYYTPLEDPGFSLVGSTIIPAYTIPTSVALETDYRIKVGVQENSPNIQDTGMLRNWNINDTTYLRTPTSRDFVLADISGTRNITVTPDYIAPKDLFKSSRDLGTNATLNRLLNLTWEFPVDSGFTYFVRLHFCEVDPNITRAGDRVFTVYLQGNVAEEQADVLKWTQNQKGLAVQRNYAVLIPKSESNNNKKVNLKIQMHAYGGNGDPFLNGLEIFKISDVVSKNLAGANPDPEKGLVNECSRETL*