AlfalfaGEDB | Msa0882220

M. sativa cultivar zhongmu-4 / Msa0882220

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0882220	KEH16541.1	78.947	114	24	0	1	114	1	114	5.76e-61	192

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0882220	G7K0A2	78.947	114	24	0	1	114	1	114	2.75e-61	192

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TFs/TRs:

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Gene ID	Type	Classification
Msa0882220	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0882210	Msa0882220	0.951340	2.512474e-109	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0882220	MtrunA17_Chr5g0421921	78.947	114	24	0	1	114	1	114	5.30e-65	192
Msa0882220	MtrunA17_Chr5g0421731	78.947	114	24	0	1	114	1	114	5.30e-65	192
Msa0882220	MtrunA17_Chr5g0421851	78.947	114	24	0	1	114	76	189	2.64e-64	193
Msa0882220	MtrunA17_Chr3g0077421	45.614	114	59	2	2	112	87	200	1.55e-26	97.4
Msa0882220	MtrunA17_Chr3g0077441	45.614	114	59	2	2	112	87	200	1.55e-26	97.4
Msa0882220	MtrunA17_Chr5g0429931	46.226	106	56	1	8	112	141	246	1.42e-25	96.3
Msa0882220	MtrunA17_Chr3g0077431	40.708	113	65	1	2	112	87	199	3.50e-25	94.0
Msa0882220	MtrunA17_Chr3g0077451	40.708	113	65	1	2	112	126	238	1.02e-24	94.0
Msa0882220	MtrunA17_Chr3g0077411	42.241	116	59	1	5	112	406	521	4.13e-21	87.0
Msa0882220	MtrunA17_Chr3g0077411	37.255	102	62	2	2	102	143	243	5.00e-12	60.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 15 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGTGGGAAGTTTATG+CGG	0.671676	6_1:-31157567	Msa0882220:CDS
AAATGATAACTATCTGTGTC+AGG	0.428925	6_1:-31157489	Msa0882220:CDS
AAATTCTGATGGAAAAGAGT+GGG	0.611424	6_1:-31157579	Msa0882220:CDS
AATGATAACTATCTGTGTCA+GGG	0.592595	6_1:-31157488	Msa0882220:CDS
AATGGCAGAAAGTGCAAGAA+TGG	0.476083	6_1:-31157810	Msa0882220:CDS
AGAAAGTGCAAGAATGGCTG+CGG	0.611130	6_1:-31157804	Msa0882220:CDS
AGTCACTAGGGGAAATAATG+CGG	0.682062	6_1:-31157744	Msa0882220:intron
ATACTGCTCCGAAATTCTGA+TGG	0.547080	6_1:-31157590	Msa0882220:CDS
ATTATGACATTTAGAACCAC+TGG	0.459360	6_1:+31157431	None:intergenic
ATTGATTGAGACAAATCCAG+TGG	0.656775	6_1:-31157447	Msa0882220:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTATAGCAAAAGTCACTAG+GGG	-	chr6_1:31157495-31157514	Msa0882220:CDS	30.0%
AGCATCAACATACTACAAAA+TGG	+	chr6_1:31157604-31157623	None:intergenic	30.0%
AAATTCTGATGGAAAAGAGT+GGG	-	chr6_1:31157671-31157690	Msa0882220:intron	30.0%
AAATGATAACTATCTGTGTC+AGG	-	chr6_1:31157761-31157780	Msa0882220:CDS	30.0%
AATGATAACTATCTGTGTCA+GGG	-	chr6_1:31157762-31157781	Msa0882220:CDS	30.0%
GAAATTCTGATGGAAAAGAG+TGG	-	chr6_1:31157670-31157689	Msa0882220:intron	35.0%
AAAAGAGTGGGAAGTTTATG+CGG	-	chr6_1:31157683-31157702	Msa0882220:intron	35.0%
GAAAAGTTTCGTACGAATGA+TGG	-	chr6_1:31157705-31157724	Msa0882220:intron	35.0%
ATTGATTGAGACAAATCCAG+TGG	-	chr6_1:31157803-31157822	Msa0882220:CDS	35.0%
AGTCACTAGGGGAAATAATG+CGG	-	chr6_1:31157506-31157525	Msa0882220:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	31157403	31157869	31157403	ID=Msa0882220;Name=Msa0882220
chr6_1	mRNA	31157403	31157869	31157403	ID=Msa0882220-mRNA-1;Parent=Msa0882220;Name=Msa0882220-mRNA-1;_AED=0.30;_eAED=0.30;_QI=38\|1\|0.5\|1\|1\|1\|2\|0\|114
chr6_1	exon	31157403	31157660	31157403	ID=Msa0882220-mRNA-1:exon:7725;Parent=Msa0882220-mRNA-1
chr6_1	exon	31157745	31157869	31157745	ID=Msa0882220-mRNA-1:exon:7724;Parent=Msa0882220-mRNA-1
chr6_1	five_prime_UTR	31157832	31157869	31157832	ID=Msa0882220-mRNA-1:five_prime_utr;Parent=Msa0882220-mRNA-1
chr6_1	CDS	31157745	31157831	31157745	ID=Msa0882220-mRNA-1:cds;Parent=Msa0882220-mRNA-1
chr6_1	CDS	31157403	31157660	31157403	ID=Msa0882220-mRNA-1:cds;Parent=Msa0882220-mRNA-1

Gene Sequence	>Msa0882220 ATGGCAGAAAGTGCAAGAATGGCTGCGGAAGCTTATCACACAGCAAATCCACATTTTATAGCAAAAGTCACTAGGGGAAATAATGCGTATGTTGATGCTACTTTTGCTTCACGCCATCTGAAAGAAAATGATTCTATACTGCTCCGAAATTCTGATGGAAAAGAGTGGGAAGTTTATGCGGAAAAGTTTCGTACGAATGATGGTCAAATGATAATGACTAAATTTCATATATTCAAAAATGATAACTATCTGTGTCAGGGAGATTACTGTGGGTTTGAATTGATTGAGACAAATCCAGTGGTTCTAAATGTCATAATGTTTCGTGTGAATGACTATGAAAATTAA
Protein sequence	>Msa0882220 MAESARMAAEAYHTANPHFIAKVTRGNNAYVDATFASRHLKENDSILLRNSDGKEWEVYAEKFRTNDGQMIMTKFHIFKNDNYLCQGDYCGFELIETNPVVLNVIMFRVNDYEN*