AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0884960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0884960 MtrunA17_Chr6g0465211 58.730 252 39 6 1 187 4 255 1.48e-79 256
Msa0884960 MtrunA17_Chr6g0465261 60.891 202 69 4 1 194 4 203 2.89e-70 213
Msa0884960 MtrunA17_Chr6g0465181 50.000 268 47 8 1 185 4 267 8.84e-62 207
Msa0884960 MtrunA17_Chr6g0465141 59.218 179 60 5 4 176 1 172 9.10e-56 177
Msa0884960 MtrunA17_Chr6g0465141 66.667 57 18 1 99 154 196 252 2.25e-13 66.6
Msa0884960 MtrunA17_Chr6g0465141 54.667 75 30 2 99 173 173 243 8.26e-11 59.7
Msa0884960 MtrunA17_Chr6g0465131 61.236 178 58 5 1 173 4 175 1.77e-53 183
Msa0884960 MtrunA17_Chr6g0465131 64.815 108 19 3 99 187 179 286 1.06e-32 124
Msa0884960 MtrunA17_Chr6g0465231 60.556 180 59 3 1 174 4 177 2.73e-53 182
Msa0884960 MtrunA17_Chr6g0465231 60.000 95 21 3 99 187 228 311 4.51e-22 93.2
Msa0884960 MtrunA17_Chr6g0464631 59.444 180 59 5 1 174 4 175 2.91e-52 180
Msa0884960 MtrunA17_Chr6g0464631 59.434 106 34 4 91 187 217 322 2.91e-26 105
Msa0884960 MtrunA17_Chr6g0465281 57.065 184 63 3 1 173 4 182 5.67e-52 168
Msa0884960 MtrunA17_Chr6g0465281 70.769 65 13 2 99 157 186 250 1.82e-17 78.6
Msa0884960 MtrunA17_Chr6g0465291 58.523 176 64 4 1 174 25 193 4.89e-51 176
Msa0884960 MtrunA17_Chr6g0465291 66.990 103 24 3 95 187 216 318 1.36e-30 117
Msa0884960 MtrunA17_Chr5g0436591 39.000 200 75 6 27 198 30 210 1.92e-28 107
Msa0884960 MtrunA17_Chr4g0004161 36.052 233 74 8 36 194 21 252 1.35e-26 106
Msa0884960 MtrunA17_Chr2g0284871 34.101 217 101 6 8 185 8 221 1.05e-24 100
Msa0884960 MtrunA17_Chr6g0455681 33.163 196 103 6 16 185 8 201 1.23e-24 97.8
Msa0884960 MtrunA17_Chr5g0439491 33.333 237 81 8 36 195 47 283 6.56e-24 98.2
Msa0884960 MtrunA17_Chr5g0420311 40.230 174 61 6 30 170 32 195 1.46e-23 94.4
Msa0884960 MtrunA17_Chr7g0219371 33.478 230 100 9 13 196 8 230 1.92e-23 97.1
Msa0884960 MtrunA17_Chr6g0455851 39.062 192 88 7 13 179 3 190 2.38e-23 97.1
Msa0884960 MtrunA17_Chr6g0455761 34.896 192 100 6 16 185 8 196 5.83e-23 95.9
Msa0884960 MtrunA17_Chr5g0429421 35.450 189 100 6 3 185 1 173 7.46e-23 95.5
Msa0884960 MtrunA17_Chr4g0019841 33.684 190 91 4 15 172 16 202 3.42e-22 93.6
Msa0884960 MtrunA17_Chr2g0284861 40.698 172 89 5 15 179 19 184 3.59e-22 93.6
Msa0884960 MtrunA17_Chr5g0420241 41.667 120 64 3 30 143 32 151 5.91e-22 88.2
Msa0884960 MtrunA17_Chr5g0439451 46.789 109 51 2 93 194 196 304 8.45e-22 92.4
Msa0884960 MtrunA17_Chr5g0439451 34.171 199 97 5 4 171 3 198 1.85e-17 80.1
Msa0884960 MtrunA17_Chr6g0455731 41.176 153 81 6 16 164 8 155 1.41e-21 91.7
Msa0884960 MtrunA17_Chr6g0455771 35.897 195 97 7 16 185 8 199 1.59e-21 91.3
Msa0884960 MtrunA17_Chr6g0455721 35.632 174 104 4 16 185 8 177 1.88e-21 91.7
Msa0884960 MtrunA17_Chr5g0439461 46.364 110 51 2 93 194 190 299 3.41e-21 90.9
Msa0884960 MtrunA17_Chr5g0439461 40.136 147 69 7 28 168 29 162 3.02e-16 76.6
Msa0884960 MtrunA17_Chr6g0455781 35.897 195 97 7 16 185 8 199 3.45e-21 90.9
Msa0884960 MtrunA17_Chr2g0284741 31.481 216 104 4 14 187 13 226 7.10e-21 89.7
Msa0884960 MtrunA17_Chr6g0455671 35.583 163 92 4 16 173 8 162 9.72e-21 89.4
Msa0884960 MtrunA17_Chr6g0455881 39.007 141 80 2 26 164 19 155 9.91e-21 89.4
Msa0884960 MtrunA17_Chr4g0022061 38.562 153 75 4 34 172 58 205 1.17e-20 88.2
Msa0884960 MtrunA17_Chr6g0463251 38.919 185 82 8 35 199 37 210 1.31e-20 89.0
Msa0884960 MtrunA17_Chr3g0087421 42.969 128 55 3 34 143 31 158 1.50e-20 89.0
Msa0884960 MtrunA17_Chr3g0097411 37.017 181 102 8 14 183 20 199 1.97e-20 88.6
Msa0884960 MtrunA17_Chr5g0439471 50.962 104 43 3 99 195 71 173 4.48e-20 86.7
Msa0884960 MtrunA17_Chr2g0284791 34.965 143 89 2 36 175 36 177 4.59e-20 83.6
Msa0884960 MtrunA17_Chr1g0203251 30.986 213 92 5 15 172 8 220 7.25e-20 87.0
Msa0884960 MtrunA17_Chr4g0019801 40.132 152 79 4 33 172 26 177 9.07e-20 86.7
Msa0884960 MtrunA17_Chr6g0455701 33.508 191 104 7 16 185 8 196 9.50e-20 86.7
Msa0884960 MtrunA17_Chr3g0106171 40.157 127 57 2 35 142 32 158 1.35e-19 86.3
Msa0884960 MtrunA17_Chr1g0161341 40.909 110 54 3 34 137 1 105 2.01e-19 79.3
Msa0884960 MtrunA17_Chr8g0367351 38.125 160 78 6 39 196 37 177 2.77e-19 85.1
Msa0884960 MtrunA17_Chr6g0455811 33.714 175 88 6 35 185 30 200 5.87e-19 84.3
Msa0884960 MtrunA17_Chr4g0008241 40.741 135 74 3 15 147 14 144 6.12e-19 84.3
Msa0884960 MtrunA17_Chr5g0403401 40.741 135 60 5 38 168 29 147 1.38e-18 83.2
Msa0884960 MtrunA17_Chr8g0380831 40.000 135 75 3 15 147 14 144 1.41e-18 83.2
Msa0884960 MtrunA17_Chr8g0380811 39.041 146 67 5 12 147 8 141 1.48e-18 83.2
Msa0884960 MtrunA17_Chr6g0455691 32.461 191 106 6 16 185 8 196 2.42e-18 82.4
Msa0884960 MtrunA17_Chr4g0019861 37.654 162 78 4 33 172 26 186 3.13e-18 82.0
Msa0884960 MtrunA17_Chr6g0455801 30.882 204 107 6 16 193 8 203 4.36e-18 81.6
Msa0884960 MtrunA17_Chr6g0463281 30.769 208 90 6 34 199 87 282 5.97e-18 81.3
Msa0884960 MtrunA17_Chr3g0104561 35.802 162 86 6 33 186 32 183 8.66e-18 80.9
Msa0884960 MtrunA17_Chr6g0464831 36.025 161 92 7 33 183 7 166 9.22e-18 78.6
Msa0884960 MtrunA17_Chr7g0219431 42.373 118 62 4 22 137 1 114 9.84e-18 75.5
Msa0884960 MtrunA17_Chr4g0022041 37.037 135 55 4 34 142 32 162 1.27e-17 80.1
Msa0884960 MtrunA17_Chr4g0019811 35.811 148 81 4 33 172 19 160 2.10e-17 78.6
Msa0884960 MtrunA17_Chr1g0202001 31.977 172 94 4 16 169 52 218 2.52e-17 79.3
Msa0884960 MtrunA17_Chr2g0304331 30.000 180 88 3 29 172 71 248 2.61e-17 79.7
Msa0884960 MtrunA17_Chr3g0097761 32.632 190 119 6 1 185 1 186 2.73e-17 79.3
Msa0884960 MtrunA17_Chr1g0190571 37.143 140 77 3 34 170 25 156 3.21e-17 79.3
Msa0884960 MtrunA17_Chr4g0008231 51.316 76 36 1 13 87 12 87 3.90e-17 74.3
Msa0884960 MtrunA17_Chr6g0463301 44.915 118 54 4 35 146 37 149 4.99e-17 78.6
Msa0884960 MtrunA17_Chr8g0366461 40.000 105 54 2 39 142 37 133 6.72e-17 78.2
Msa0884960 MtrunA17_Chr5g0441481 31.884 207 89 9 36 194 8 210 1.80e-16 75.1
Msa0884960 MtrunA17_Chr3g0097321 33.735 166 101 6 28 185 26 190 2.30e-16 76.6
Msa0884960 MtrunA17_Chr5g0408621 38.060 134 67 4 38 169 76 195 2.89e-16 76.6
Msa0884960 MtrunA17_Chr3g0097461 34.177 158 98 5 33 185 31 187 3.15e-16 76.3
Msa0884960 MtrunA17_Chr3g0097551 35.714 126 78 3 28 151 26 150 4.11e-16 72.4
Msa0884960 MtrunA17_Chr3g0097471 33.099 142 87 3 33 169 32 170 1.09e-15 72.0
Msa0884960 MtrunA17_Chr3g0093881 32.632 190 103 7 9 185 18 195 1.26e-15 74.7
Msa0884960 MtrunA17_Chr3g0087811 30.769 195 91 5 11 173 8 190 2.52e-15 73.2
Msa0884960 MtrunA17_Chr4g0058861 47.000 100 44 3 44 142 39 130 5.34e-15 72.8
Msa0884960 MtrunA17_Chr8g0362951 33.136 169 75 6 27 168 19 176 1.05e-14 70.1
Msa0884960 MtrunA17_Chr5g0408611 37.121 132 67 4 38 167 29 146 1.08e-14 72.0
Msa0884960 MtrunA17_Chr3g0097701 35.503 169 95 8 29 185 27 193 1.10e-14 72.0
Msa0884960 MtrunA17_Chr4g0032941 33.529 170 74 5 36 171 24 188 1.11e-14 71.2
Msa0884960 MtrunA17_Chr3g0097311 40.187 107 60 4 26 129 23 128 1.69e-14 71.2
Msa0884960 MtrunA17_Chr7g0216791 37.857 140 54 5 35 143 12 149 3.04e-14 69.7
Msa0884960 MtrunA17_Chr3g0095011 32.727 165 99 6 32 185 28 191 3.24e-14 70.5
Msa0884960 MtrunA17_Chr8g0362861 32.044 181 102 8 27 198 19 187 4.74e-14 68.2
Msa0884960 MtrunA17_Chr3g0097271 33.544 158 92 6 33 185 32 181 5.41e-14 69.3
Msa0884960 MtrunA17_Chr3g0113951 36.296 135 65 5 19 148 127 245 5.84e-14 69.7
Msa0884960 MtrunA17_Chr3g0097361 32.353 170 96 8 26 185 24 184 7.60e-14 69.3
Msa0884960 MtrunA17_Chr3g0097261 36.641 131 74 5 28 152 26 153 1.05e-13 68.9
Msa0884960 MtrunA17_Chr6g0455791 37.179 156 73 5 45 179 112 263 1.94e-13 67.4
Msa0884960 MtrunA17_Chr2g0304281 29.480 173 84 3 36 172 32 202 2.18e-13 68.2
Msa0884960 MtrunA17_Chr8g0363261 29.878 164 101 5 27 187 19 171 2.22e-13 67.8
Msa0884960 MtrunA17_Chr7g0216731 34.559 136 60 4 35 143 41 174 2.27e-13 67.8
Msa0884960 MtrunA17_Chr5g0419221 42.857 98 46 3 38 133 41 130 2.40e-13 68.2
Msa0884960 MtrunA17_Chr8g0363531 30.387 181 105 8 27 198 39 207 3.31e-13 67.0
Msa0884960 MtrunA17_Chr3g0097651 33.333 159 92 7 33 185 31 181 3.58e-13 67.4
Msa0884960 MtrunA17_Chr4g0023641 32.143 168 100 6 19 180 17 176 3.93e-13 66.2
Msa0884960 MtrunA17_Chr8g0363021 33.088 136 80 4 15 147 24 151 4.76e-13 64.3
Msa0884960 MtrunA17_Chr2g0278871 37.500 136 70 4 40 173 35 157 5.57e-13 66.6
Msa0884960 MtrunA17_Chr5g0407241 37.313 134 68 4 38 169 47 166 5.99e-13 67.0
Msa0884960 MtrunA17_Chr8g0362881 32.727 165 73 7 27 161 19 175 7.10e-13 65.1
Msa0884960 MtrunA17_Chr3g0097341 31.875 160 92 5 33 185 32 181 7.12e-13 66.6
Msa0884960 MtrunA17_Chr8g0363691 33.121 157 81 6 16 169 18 153 7.55e-13 66.6
Msa0884960 MtrunA17_Chr7g0215761 35.329 167 72 8 35 172 29 188 7.69e-13 66.6
Msa0884960 MtrunA17_Chr4g0013511 33.333 168 74 6 27 167 19 175 1.08e-12 65.1
Msa0884960 MtrunA17_Chr5g0408561 39.796 98 49 3 38 133 30 119 1.16e-12 65.9
Msa0884960 MtrunA17_Chr2g0304481 29.480 173 84 3 36 172 32 202 1.16e-12 65.9
Msa0884960 MtrunA17_Chr8g0363451 32.192 146 85 5 27 169 39 173 1.17e-12 65.5
Msa0884960 MtrunA17_Chr7g0216801 35.971 139 56 5 35 142 52 188 1.18e-12 65.9
Msa0884960 MtrunA17_Chr6g0464211 35.714 126 71 3 38 161 41 158 1.22e-12 65.9
Msa0884960 MtrunA17_Chr7g0232171 33.793 145 78 3 38 164 41 185 1.31e-12 65.9
Msa0884960 MtrunA17_Chr7g0216771 36.429 140 56 5 35 143 34 171 1.40e-12 65.5
Msa0884960 MtrunA17_Chr3g0097721 33.333 126 63 5 9 128 5 115 1.42e-12 65.5
Msa0884960 MtrunA17_Chr8g0363061 31.098 164 99 5 27 187 19 171 1.66e-12 65.5
Msa0884960 MtrunA17_Chr3g0097291 33.566 143 76 5 33 169 7 136 1.87e-12 65.1
Msa0884960 MtrunA17_Chr7g0216641 35.714 140 57 5 35 143 34 171 2.21e-12 65.1
Msa0884960 MtrunA17_Chr8g0372351 39.474 114 62 5 36 142 30 143 2.28e-12 64.7
Msa0884960 MtrunA17_Chr8g0351951 26.154 195 109 4 33 195 19 210 2.76e-12 64.3
Msa0884960 MtrunA17_Chr1g0176281 31.677 161 96 5 12 169 5 154 2.81e-12 63.2
Msa0884960 MtrunA17_Chr1g0176291 31.677 161 96 5 12 169 5 154 2.81e-12 63.2
Msa0884960 MtrunA17_Chr8g0372311 39.474 114 62 5 36 142 30 143 2.81e-12 64.3
Msa0884960 MtrunA17_Chr8g0372321 39.474 114 62 5 36 142 30 143 3.32e-12 64.3
Msa0884960 MtrunA17_Chr8g0363631 32.143 168 76 6 27 167 40 196 3.49e-12 63.5
Msa0884960 MtrunA17_Chr8g0362411 32.192 146 85 5 27 169 19 153 3.67e-12 63.2
Msa0884960 MtrunA17_Chr3g0097511 35.556 90 48 3 26 113 23 104 4.27e-12 61.2
Msa0884960 MtrunA17_Chr8g0363131 32.886 149 65 5 27 148 19 159 4.33e-12 63.5
Msa0884960 MtrunA17_Chr0c09g0493541 33.929 168 83 7 11 170 14 161 5.05e-12 63.2
Msa0884960 MtrunA17_Chr8g0363111 32.192 146 85 5 27 169 19 153 5.07e-12 64.3
Msa0884960 MtrunA17_Chr8g0363681 31.765 170 78 6 27 169 20 178 5.19e-12 63.2
Msa0884960 MtrunA17_Chr2g0278891 39.604 101 49 3 44 142 36 126 7.65e-12 63.5
Msa0884960 MtrunA17_Chr2g0278831 35.664 143 76 4 11 151 9 137 8.18e-12 63.5
Msa0884960 MtrunA17_Chr5g0446201 32.847 137 63 5 36 143 63 199 8.18e-12 62.0
Msa0884960 MtrunA17_Chr7g0216701 37.931 145 65 6 35 164 34 168 1.01e-11 63.2
Msa0884960 MtrunA17_Chr5g0393671 33.088 136 77 4 38 171 26 149 1.05e-11 61.6
Msa0884960 MtrunA17_Chr7g0232211 33.103 145 79 3 39 165 33 177 1.18e-11 63.2
Msa0884960 MtrunA17_Chr3g0127671 35.433 127 73 2 39 164 68 186 1.21e-11 63.2
Msa0884960 MtrunA17_Chr8g0363311 31.548 168 77 6 27 167 40 196 1.22e-11 62.0
Msa0884960 MtrunA17_Chr3g0127701 32.903 155 88 4 12 164 4 144 1.26e-11 63.2
Msa0884960 MtrunA17_Chr8g0363251 31.548 168 77 6 27 167 40 196 1.27e-11 62.0
Msa0884960 MtrunA17_Chr8g0362931 32.143 168 76 6 27 167 39 195 1.28e-11 63.2
Msa0884960 MtrunA17_Chr8g0363381 31.548 168 77 6 27 167 36 192 1.30e-11 61.6
Msa0884960 MtrunA17_Chr8g0363231 36.207 116 63 4 35 147 27 134 1.40e-11 61.6
Msa0884960 MtrunA17_Chr8g0386631 32.609 138 79 4 37 171 25 151 1.44e-11 62.8
Msa0884960 MtrunA17_Chr5g0408811 36.923 130 72 3 38 165 17 138 1.46e-11 62.8
Msa0884960 MtrunA17_Chr8g0361231 32.716 162 91 6 15 173 8 154 1.60e-11 62.8
Msa0884960 MtrunA17_Chr5g0439291 35.172 145 74 5 39 164 44 187 1.67e-11 62.8
Msa0884960 MtrunA17_Chr7g0217371 33.784 148 64 6 28 143 26 171 1.67e-11 62.8
Msa0884960 MtrunA17_Chr5g0413291 36.301 146 73 5 37 165 31 173 1.82e-11 62.4
Msa0884960 MtrunA17_Chr4g0051961 39.450 109 56 3 36 142 28 128 2.15e-11 62.4
Msa0884960 MtrunA17_Chr7g0214121 29.293 198 108 7 1 196 33 200 2.15e-11 61.2
Msa0884960 MtrunA17_Chr3g0095181 33.577 137 67 5 11 140 16 135 3.25e-11 58.9
Msa0884960 MtrunA17_Chr2g0284121 29.412 187 81 6 16 171 22 188 4.14e-11 61.6
Msa0884960 MtrunA17_Chr8g0363001 31.757 148 83 6 27 169 19 153 4.45e-11 60.1
Msa0884960 MtrunA17_Chr8g0362961 30.822 146 87 5 27 169 20 154 5.31e-11 61.2
Msa0884960 MtrunA17_Chr2g0284161 33.125 160 89 4 15 167 11 159 5.57e-11 60.5
Msa0884960 MtrunA17_Chr1g0200911 38.462 117 60 4 43 156 34 141 6.28e-11 60.8
Msa0884960 MtrunA17_Chr1g0169841 34.646 127 61 5 44 148 39 165 6.77e-11 60.8
Msa0884960 MtrunA17_Chr7g0216941 34.507 142 74 4 35 164 27 161 6.85e-11 60.5
Msa0884960 MtrunA17_Chr5g0409241 31.325 166 78 5 4 143 1 156 9.35e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0884960 AT2G34930.1 38.776 147 84 4 30 171 27 172 6.84e-17 78.6
Msa0884960 AT5G46330.2 38.168 131 68 4 35 161 25 146 2.07e-15 74.3
Msa0884960 AT5G46330.1 38.168 131 68 4 35 161 25 146 2.07e-15 74.3
Msa0884960 AT3G20820.1 30.952 168 81 4 36 171 20 184 4.68e-14 70.1
Msa0884960 AT3G28040.1 35.948 153 70 4 42 170 38 186 6.20e-14 70.1
Msa0884960 AT3G28890.1 29.121 182 96 4 16 164 17 198 4.45e-13 67.4
Msa0884960 AT3G28890.2 29.121 182 96 4 16 164 17 198 4.45e-13 67.4
Msa0884960 AT1G33600.1 30.556 180 87 6 15 169 15 181 2.28e-12 65.5
Msa0884960 AT1G33600.2 30.556 180 87 6 15 169 15 181 2.28e-12 65.5
Msa0884960 AT2G02220.1 36.232 138 74 4 7 142 6 131 3.97e-12 64.7
Msa0884960 AT3G12145.1 35.000 140 70 7 36 170 24 147 4.73e-12 63.9
Msa0884960 AT3G47110.1 30.714 140 87 3 14 151 14 145 5.35e-12 64.3
Msa0884960 AT5G62710.1 34.091 132 74 4 42 171 29 149 7.90e-12 63.9
Msa0884960 AT1G33670.1 30.601 183 89 7 15 170 13 184 8.62e-12 63.5
Msa0884960 AT1G33610.1 29.032 186 99 6 9 167 1 180 8.63e-12 63.5
Msa0884960 AT3G47570.1 37.333 150 66 6 38 167 23 164 1.01e-11 63.5
Msa0884960 AT3G05360.1 29.570 186 101 5 11 171 12 192 1.18e-11 63.5
Msa0884960 AT3G05660.1 35.329 167 73 5 36 171 30 192 1.52e-11 63.2
Msa0884960 AT3G25560.2 31.579 152 90 4 35 182 37 178 1.63e-11 62.8
Msa0884960 AT3G25560.1 33.557 149 80 5 35 174 37 175 2.21e-11 62.4
Msa0884960 AT3G25560.3 33.557 149 80 5 35 174 37 175 2.83e-11 62.4
Msa0884960 AT3G11080.2 30.270 185 93 4 16 164 18 202 3.65e-11 62.0
Msa0884960 AT3G11080.1 30.270 185 93 4 16 164 18 202 3.65e-11 62.0
Msa0884960 AT5G23400.1 34.167 120 73 4 27 142 22 139 3.68e-11 62.0
Msa0884960 AT4G18640.1 36.232 138 65 6 37 167 26 147 3.73e-11 62.0
Msa0884960 AT5G20480.1 32.847 137 78 4 38 171 29 154 3.80e-11 62.0
Msa0884960 AT5G20480.2 32.847 137 78 4 38 171 29 154 3.80e-11 62.0
Msa0884960 AT2G26330.1 33.544 158 84 4 27 164 13 169 5.67e-11 61.6
Msa0884960 AT1G71400.1 28.643 199 103 8 15 188 17 201 5.81e-11 61.2
Msa0884960 AT1G72180.1 34.074 135 73 3 40 173 34 153 7.82e-11 61.2

Find 40 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAAGATAAAGCTCTTTA+AGG 0.141094 6_1:+39219663 None:intergenic
GCTGGCACTCCTTCAATTTA+AGG 0.234035 6_1:-39219948 Msa0884960:CDS
GTAATTATGCTCAACCTTCA+TGG 0.312735 6_1:-39219830 Msa0884960:CDS
ACCATTTGCAACCAACTATC+AGG 0.314145 6_1:+39219548 None:intergenic
AAACTTAGTTCTCTCAGTTT+TGG 0.323889 6_1:+39219514 None:intergenic
AACAATATTTCTCATCTGTT+TGG 0.324745 6_1:-39220032 Msa0884960:CDS
CATTTGACATGTTGTCATCT+TGG 0.348502 6_1:-39219910 Msa0884960:CDS
AACCTTAAAGAGCTTTATCT+TGG 0.363323 6_1:-39219665 Msa0884960:CDS
CTTCAATTTAAGGCTGGCCT+TGG 0.428575 6_1:-39219938 Msa0884960:CDS
CAAGATGACAACATGTCAAA+TGG 0.461468 6_1:+39219911 None:intergenic
TTTGCTCAAAAGAGGTCGTT+AGG 0.462068 6_1:-39219988 Msa0884960:CDS
GCACTCCTTCAATTTAAGGC+TGG 0.478360 6_1:-39219944 Msa0884960:CDS
CAAGGCCAGCCTTAAATTGA+AGG 0.486764 6_1:+39219939 None:intergenic
TGCTGCGAATGGTATGGAAT+TGG 0.493083 6_1:-39219875 Msa0884960:CDS
CCAGCTTGTTTGCTCAAAAG+AGG 0.494443 6_1:-39219996 Msa0884960:CDS
TCCTGATAGTTGGTTGCAAA+TGG 0.497415 6_1:-39219549 Msa0884960:CDS
ATACTGACTCCATGTGAAGA+TGG 0.506721 6_1:+39219576 None:intergenic
AATGACAACAGAGGTCTAGA+TGG 0.515349 6_1:-39219791 Msa0884960:CDS
GTGCATTCAAAGTGAGAGGC+TGG 0.520512 6_1:-39219966 Msa0884960:CDS
GAAGATTGCTGCGAATGGTA+TGG 0.521402 6_1:-39219881 Msa0884960:CDS
GTAAGCTTTCCAAGTTGAGA+TGG 0.534824 6_1:+39219689 None:intergenic
TTCTTTAACCCATCTTCACA+TGG 0.537186 6_1:-39219585 Msa0884960:CDS
GAACTAAGTTTACAAAGTTG+TGG 0.544896 6_1:-39219502 Msa0884960:CDS
CAGTATCTAATCCTGATAGT+TGG 0.546534 6_1:-39219559 Msa0884960:CDS
TATAATCATAATAACCATGA+AGG 0.550240 6_1:+39219816 None:intergenic
TACTGACTCCATGTGAAGAT+GGG 0.551864 6_1:+39219577 None:intergenic
GGAAGCATTCCATCTCAACT+TGG 0.565265 6_1:-39219698 Msa0884960:CDS
AATGGATAAAATGATCAGAA+AGG 0.571001 6_1:+39219479 None:intergenic
ATTGGTTGCAGCAACCTCAC+AGG 0.574637 6_1:-39219857 Msa0884960:CDS
CGACAGAAGATTGCTGCGAA+TGG 0.577784 6_1:-39219886 Msa0884960:CDS
GGTGTGATTTCTCATCGACT+TGG 0.582316 6_1:-39219770 Msa0884960:CDS
GAGATCAAGAAATTGCAAGT+TGG 0.584491 6_1:+39219739 None:intergenic
GATAGTTGGTTGCAAATGGT+TGG 0.610586 6_1:-39219545 Msa0884960:CDS
TTTGCTCTGACAATTGACAG+TGG 0.638691 6_1:-39219632 Msa0884960:CDS
TTAGGTGCATTCAAAGTGAG+AGG 0.642069 6_1:-39219970 Msa0884960:CDS
GATTATATTAATGACAACAG+AGG 0.687487 6_1:-39219800 Msa0884960:CDS
GCTCTGACAATTGACAGTGG+AGG 0.692478 6_1:-39219629 Msa0884960:CDS
CAATTGACAGTGGAGGTCAG+TGG 0.727682 6_1:-39219622 Msa0884960:CDS
ACATGTCAAATGGATCACCA+AGG 0.750683 6_1:+39219921 None:intergenic
GCATAATTACATGACCTGTG+AGG 0.782227 6_1:+39219843 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATCGATTTTAAACAAAA+TGG + chr6_1:39219511-39219530 None:intergenic 20.0%
!! TATAATCATAATAACCATGA+AGG + chr6_1:39219750-39219769 None:intergenic 20.0%
! AACAATATTTCTCATCTGTT+TGG - chr6_1:39219531-39219550 Msa0884960:CDS 25.0%
! GATTATATTAATGACAACAG+AGG - chr6_1:39219763-39219782 Msa0884960:CDS 25.0%
! AACCTTAAAGAGCTTTATCT+TGG - chr6_1:39219898-39219917 Msa0884960:CDS 30.0%
AACCAAGATAAAGCTCTTTA+AGG + chr6_1:39219903-39219922 None:intergenic 30.0%
!! AAACTTAGTTCTCTCAGTTT+TGG + chr6_1:39220052-39220071 None:intergenic 30.0%
GAACTAAGTTTACAAAGTTG+TGG - chr6_1:39220061-39220080 Msa0884960:CDS 30.0%
CAAGATGACAACATGTCAAA+TGG + chr6_1:39219655-39219674 None:intergenic 35.0%
CATTTGACATGTTGTCATCT+TGG - chr6_1:39219653-39219672 Msa0884960:CDS 35.0%
GTAATTATGCTCAACCTTCA+TGG - chr6_1:39219733-39219752 Msa0884960:CDS 35.0%
GAGATCAAGAAATTGCAAGT+TGG + chr6_1:39219827-39219846 None:intergenic 35.0%
TGCTTCCTTCGAAAAAATTG+TGG + chr6_1:39219852-39219871 None:intergenic 35.0%
TTCTTTAACCCATCTTCACA+TGG - chr6_1:39219978-39219997 Msa0884960:CDS 35.0%
CAGTATCTAATCCTGATAGT+TGG - chr6_1:39220004-39220023 Msa0884960:CDS 35.0%
TTTGCTCAAAAGAGGTCGTT+AGG - chr6_1:39219575-39219594 Msa0884960:CDS 40.0%
TTAGGTGCATTCAAAGTGAG+AGG - chr6_1:39219593-39219612 Msa0884960:CDS 40.0%
ACATGTCAAATGGATCACCA+AGG + chr6_1:39219645-39219664 None:intergenic 40.0%
GCATAATTACATGACCTGTG+AGG + chr6_1:39219723-39219742 None:intergenic 40.0%
AATGACAACAGAGGTCTAGA+TGG - chr6_1:39219772-39219791 Msa0884960:CDS 40.0%
!!! CTCAGCCACAATTTTTTCGA+AGG - chr6_1:39219844-39219863 Msa0884960:CDS 40.0%
! GTAAGCTTTCCAAGTTGAGA+TGG + chr6_1:39219877-39219896 None:intergenic 40.0%
TTTGCTCTGACAATTGACAG+TGG - chr6_1:39219931-39219950 Msa0884960:CDS 40.0%
!! TACTGACTCCATGTGAAGAT+GGG + chr6_1:39219989-39220008 None:intergenic 40.0%
!! ATACTGACTCCATGTGAAGA+TGG + chr6_1:39219990-39220009 None:intergenic 40.0%
TCCTGATAGTTGGTTGCAAA+TGG - chr6_1:39220014-39220033 Msa0884960:CDS 40.0%
ACCATTTGCAACCAACTATC+AGG + chr6_1:39220018-39220037 None:intergenic 40.0%
GATAGTTGGTTGCAAATGGT+TGG - chr6_1:39220018-39220037 Msa0884960:CDS 40.0%
! CCTCTTTTGAGCAAACAAGC+TGG + chr6_1:39219570-39219589 None:intergenic 45.0%
! CCAGCTTGTTTGCTCAAAAG+AGG - chr6_1:39219567-39219586 Msa0884960:CDS 45.0%
GCTGGCACTCCTTCAATTTA+AGG - chr6_1:39219615-39219634 Msa0884960:CDS 45.0%
! GCACTCCTTCAATTTAAGGC+TGG - chr6_1:39219619-39219638 Msa0884960:CDS 45.0%
CAAGGCCAGCCTTAAATTGA+AGG + chr6_1:39219627-39219646 None:intergenic 45.0%
!! CTTCAATTTAAGGCTGGCCT+TGG - chr6_1:39219625-39219644 Msa0884960:CDS 45.0%
GAAGATTGCTGCGAATGGTA+TGG - chr6_1:39219682-39219701 Msa0884960:CDS 45.0%
TGCTGCGAATGGTATGGAAT+TGG - chr6_1:39219688-39219707 Msa0884960:CDS 45.0%
! GGTGTGATTTCTCATCGACT+TGG - chr6_1:39219793-39219812 Msa0884960:CDS 45.0%
!! GGAAGCATTCCATCTCAACT+TGG - chr6_1:39219865-39219884 Msa0884960:CDS 45.0%
GTGCATTCAAAGTGAGAGGC+TGG - chr6_1:39219597-39219616 Msa0884960:CDS 50.0%
CGACAGAAGATTGCTGCGAA+TGG - chr6_1:39219677-39219696 Msa0884960:CDS 50.0%
ATTGGTTGCAGCAACCTCAC+AGG - chr6_1:39219706-39219725 Msa0884960:CDS 50.0%
GCTCTGACAATTGACAGTGG+AGG - chr6_1:39219934-39219953 Msa0884960:CDS 50.0%
CAATTGACAGTGGAGGTCAG+TGG - chr6_1:39219941-39219960 Msa0884960:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 39219493 39220092 39219493 ID=Msa0884960;Name=Msa0884960
chr6_1 mRNA 39219493 39220092 39219493 ID=Msa0884960-mRNA-1;Parent=Msa0884960;Name=Msa0884960-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|199
chr6_1 exon 39219493 39220092 39219493 ID=Msa0884960-mRNA-1:exon:9059;Parent=Msa0884960-mRNA-1
chr6_1 CDS 39219493 39220092 39219493 ID=Msa0884960-mRNA-1:cds;Parent=Msa0884960-mRNA-1
Gene Sequence

>Msa0884960

ATGTCAATCATGAATCCATTTTGTTTAAAATCGATTCAAACAATATTTCTCATCTGTTTGGTGTTGCAAGCAGACCAGCTTGTTTGCTCAAAAGAGGTCGTTAGGTGCATTCAAAGTGAGAGGCTGGCACTCCTTCAATTTAAGGCTGGCCTTGGTGATCCATTTGACATGTTGTCATCTTGGACGACAGAAGATTGCTGCGAATGGTATGGAATTGGTTGCAGCAACCTCACAGGTCATGTAATTATGCTCAACCTTCATGGTTATTATGATTATATTAATGACAACAGAGGTCTAGATGGTGTGATTTCTCATCGACTTGGAGATCTCTCCAACTTGCAATTTCTTGATCTCAGCCACAATTTTTTCGAAGGAAGCATTCCATCTCAACTTGGAAAGCTTACGAACCTTAAAGAGCTTTATCTTGGTTGCAGCTTGTTTGCTCTGACAATTGACAGTGGAGGTCAGTGGTTGTCTAATCTTACTTCTTTAACCCATCTTCACATGGAGTCAGTATCTAATCCTGATAGTTGGTTGCAAATGGTTGGTAAAGTTACCAAAACTGAGAGAACTAAGTTTACAAAGTTGTGGCCTTTCTGA
Protein sequence

>Msa0884960

MSIMNPFCLKSIQTIFLICLVLQADQLVCSKEVVRCIQSERLALLQFKAGLGDPFDMLSSWTTEDCCEWYGIGCSNLTGHVIMLNLHGYYDYINDNRGLDGVISHRLGDLSNLQFLDLSHNFFEGSIPSQLGKLTNLKELYLGCSLFALTIDSGGQWLSNLTSLTHLHMESVSNPDSWLQMVGKVTKTERTKFTKLWPF*