AlfalfaGEDB | Msa0886960

M. sativa cultivar zhongmu-4 / Msa0886960

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886960	XP_013452170.2	83.714	350	31	3	1	338	1	336	0.0	580

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886960	sp\|Q9T072\|BH025_ARATH	40.756	238	115	7	105	336	111	328	1.34e-43	155

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886960	A0A072UAR9	83.714	350	31	3	1	338	1	336	0.0	579

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TFs/TRs:

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Gene ID	Type	Classification
Msa0886960	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0886960	Msa0988460	0.898244	6.412368e-77	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886960	MtrunA17_Chr6g0468491	82.622	328	31	3	23	338	1	314	0.0	536
Msa0886960	MtrunA17_Chr6g0468461	76.190	336	45	6	1	321	1	316	2.99e-170	477
Msa0886960	MtrunA17_Chr2g0307131	67.222	360	90	3	1	337	1	355	1.25e-161	455
Msa0886960	MtrunA17_Chr2g0307141	64.932	365	96	5	1	338	1	360	4.10e-151	429
Msa0886960	MtrunA17_Chr4g0033391	62.216	352	84	9	1	336	1	319	2.40e-136	390
Msa0886960	MtrunA17_Chr4g0033291	60.598	368	76	7	1	338	1	329	1.49e-133	383
Msa0886960	MtrunA17_Chr4g0033281	63.173	353	86	7	1	337	1	325	3.65e-131	377
Msa0886960	MtrunA17_Chr4g0054581	56.443	388	110	7	1	338	1	379	2.23e-125	364
Msa0886960	MtrunA17_Chr4g0054621	54.237	354	134	11	1	338	1	342	1.34e-113	333
Msa0886960	MtrunA17_Chr7g0225901	60.678	295	57	5	23	313	1	240	7.85e-113	328

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886960	AT4G37850.2	40.756	238	115	7	105	336	111	328	1.36e-44	155
Msa0886960	AT4G37850.1	40.756	238	115	7	105	336	111	328	1.36e-44	155
Msa0886960	AT2G22760.1	44.615	195	95	6	151	333	102	295	2.92e-44	154
Msa0886960	AT2G22750.3	32.964	361	179	13	7	335	3	332	2.39e-37	137
Msa0886960	AT2G22760.2	43.678	174	86	5	151	313	102	274	3.53e-37	135
Msa0886960	AT2G22750.2	43.367	196	103	3	146	335	111	304	4.72e-37	135
Msa0886960	AT2G22750.5	43.367	196	103	3	146	335	135	328	5.16e-37	135
Msa0886960	AT2G22750.4	32.964	361	178	14	7	335	3	331	4.15e-36	133
Msa0886960	AT2G22750.1	43.367	196	102	4	146	335	111	303	8.80e-36	132
Msa0886960	AT2G22770.1	34.728	239	131	5	110	331	87	317	3.88e-35	130

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Find 54 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTCTCGTACTCATCA+TGG	0.528225	6_1:+47612904	Msa0886960:CDS
AAACAACAATGTTTCACTTC+AGG	0.379077	6_1:+47612625	Msa0886960:CDS
AAATTATGGTAGAGATTCAA+AGG	0.513560	6_1:+47613417	Msa0886960:CDS
AACAACAATGTTTCACTTCA+GGG	0.435505	6_1:+47612626	Msa0886960:CDS
AACTGAACAGATGATTTAGA+TGG	0.536454	6_1:-47612799	None:intergenic
AATAACAGTGTCTTACCTTT+TGG	0.250719	6_1:+47613458	Msa0886960:CDS
AATGCTGTCATCACATGATG+AGG	0.580177	6_1:-47613294	None:intergenic
AATTAGTAGTGGGTGAATTT+GGG	0.256712	6_1:-47612846	None:intergenic
ACCCAATTTGAATGAGACAA+TGG	0.623594	6_1:+47612147	Msa0886960:exon
ACGTATCATCAACCAACAGC+TGG	0.606784	6_1:+47612194	Msa0886960:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCATTGTCTCATTCAAATT+GGG	-	chr6_1:47612151-47612170	None:intergenic	30.0%
TTCCATTGTCTCATTCAAAT+TGG	-	chr6_1:47612152-47612171	None:intergenic	30.0%
AAACAACAATGTTTCACTTC+AGG	+	chr6_1:47612625-47612644	Msa0886960:CDS	30.0%
AACAACAATGTTTCACTTCA+GGG	+	chr6_1:47612626-47612645	Msa0886960:CDS	30.0%
AACTGAACAGATGATTTAGA+TGG	-	chr6_1:47612802-47612821	None:intergenic	30.0%
TCAGTTTCAAATTCCATCTT+TGG	+	chr6_1:47612816-47612835	Msa0886960:CDS	30.0%
TACTAATTCCTCACAAAACT+TGG	+	chr6_1:47612861-47612880	Msa0886960:CDS	30.0%
TAATGTACCTTCTTAAGGTT+AGG	-	chr6_1:47613006-47613025	None:intergenic	30.0%
TCCATTGTCTCATTCAAATT+GGG	-	chr6_1:47612151-47612170	None:intergenic	30.0%
TTCCATTGTCTCATTCAAAT+TGG	-	chr6_1:47612152-47612171	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	47612104	47614097	47612104	ID=Msa0886960;Name=Msa0886960
chr6_1	mRNA	47612104	47614097	47612104	ID=Msa0886960-mRNA-1;Parent=Msa0886960;Name=Msa0886960-mRNA-1;_AED=0.01;_eAED=0.01;_QI=62\|1\|1\|1\|1\|1\|4\|232\|338
chr6_1	exon	47612104	47612228	47612104	ID=Msa0886960-mRNA-1:exon:9966;Parent=Msa0886960-mRNA-1
chr6_1	exon	47612514	47613017	47612514	ID=Msa0886960-mRNA-1:exon:9967;Parent=Msa0886960-mRNA-1
chr6_1	exon	47613146	47613514	47613146	ID=Msa0886960-mRNA-1:exon:9968;Parent=Msa0886960-mRNA-1
chr6_1	exon	47613785	47614097	47613785	ID=Msa0886960-mRNA-1:exon:9969;Parent=Msa0886960-mRNA-1
chr6_1	five_prime_UTR	47612104	47612165	47612104	ID=Msa0886960-mRNA-1:five_prime_utr;Parent=Msa0886960-mRNA-1
chr6_1	CDS	47612166	47612228	47612166	ID=Msa0886960-mRNA-1:cds;Parent=Msa0886960-mRNA-1
chr6_1	CDS	47612514	47613017	47612514	ID=Msa0886960-mRNA-1:cds;Parent=Msa0886960-mRNA-1
chr6_1	CDS	47613146	47613514	47613146	ID=Msa0886960-mRNA-1:cds;Parent=Msa0886960-mRNA-1
chr6_1	CDS	47613785	47613865	47613785	ID=Msa0886960-mRNA-1:cds;Parent=Msa0886960-mRNA-1
chr6_1	three_prime_UTR	47613866	47614097	47613866	ID=Msa0886960-mRNA-1:three_prime_utr;Parent=Msa0886960-mRNA-1

Gene Sequence

>Msa0886960

ATGGAAGAAATCAACAACACAACAATGAACGTATCATCAACCAACAGCTGGTTATCTGATTTAGAAATGGATGAATACAATTTATTTCCTGAGAAAAGCAACCTGAATTTCCTTGATACAGATGTGGTAGAGTTTCTCTCACATGACATAGGTAATGTATTCCAAGAACAAAACAAACAACAATGTTTCACTTCAGGGTGCACTCCCGCATCTCTTAGTAACTCATCAAGTTATGAAACAAATTTGAACTCTTTTGACTTTGATTTTGATAAGCCAAATATGGAGCTGAAAAAAATTGATCATAGCAACAAAATCAATGAAACCTTTTCACCAAAACTTTCTTCATCACCATCTAAATCATCTGTTCAGTTTCAAATTCCATCTTTGGACAACACCCCAAATTCACCCACTACTAATTCCTCACAAAACTTGGAAACAAAAACCTCAGAGAGCAAAAGGTCTCGTACTCATCATGGTCAAGATCATATCATGGCTGAGAGAAAGAGAAGAGAGAAACTCACTCAGAACTTCATTGCTCTTGCGGCTCTTGTTCCTAACCTTAAGAAGGTGGACAAGTATTCTGTATTAGTTGACACCATCAAATACTTAAAAGAGCTTAAAAAGCGTCTGAAAGTTTTGGAAGAACAAAATGAGAAAACAAAAATAGAGTCACCGATAGTCTTGACCAAACCAGGCCTCTGCAACGATGACAACCCCTCATCATGTGATGACAGCATTGATTGTGTTGTTGGTTTACCATTTCAAGTGGAAGCAAAAGTCTTAGGAAAACATATGTTGATTCGGATCCAATGTCAAGAGCACAGGGGCCTTCTAGTTAAAATTATGGTAGAGATTCAAAGGTTTCAACTATTTGTTATCAATAACAGTGTCTTACCTTTTGGAGATTCTATCCTCGACATTACTATTATTGCTCAGTTGGGTGAAGGCTACAGCATGAGCATAAAGGAACTTGTGAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCGTAA

Protein sequence

>Msa0886960

MEEINNTTMNVSSTNSWLSDLEMDEYNLFPEKSNLNFLDTDVVEFLSHDIGNVFQEQNKQQCFTSGCTPASLSNSSSYETNLNSFDFDFDKPNMELKKIDHSNKINETFSPKLSSSPSKSSVQFQIPSLDNTPNSPTTNSSQNLETKTSESKRSRTHHGQDHIMAERKRREKLTQNFIALAALVPNLKKVDKYSVLVDTIKYLKELKKRLKVLEEQNEKTKIESPIVLTKPGLCNDDNPSSCDDSIDCVVGLPFQVEAKVLGKHMLIRIQCQEHRGLLVKIMVEIQRFQLFVINNSVLPFGDSILDITIIAQLGEGYSMSIKELVKNLRMAALKFMSS*