AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0897160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0897160 MtrunA17_Chr6g0482641 88.421 285 30 2 1 285 1 282 0.0 529
Msa0897160 MtrunA17_Chr6g0475211 54.333 427 123 8 42 431 134 525 2.22e-142 417
Msa0897160 MtrunA17_Chr3g0078341 54.567 427 122 8 42 431 130 521 1.95e-141 414
Msa0897160 MtrunA17_Chr6g0482631 78.070 114 15 1 328 431 1 114 1.73e-51 169
Msa0897160 MtrunA17_Chr1g0149891 35.000 320 120 7 123 432 91 332 2.76e-45 160
Msa0897160 MtrunA17_Chr6g0450581 33.230 322 161 8 119 432 27 302 1.59e-42 152
Msa0897160 MtrunA17_Chr1g0149901 35.106 282 96 6 161 432 1 205 1.18e-38 139
Msa0897160 MtrunA17_Chr6g0476071 47.826 161 60 4 145 303 3 141 1.12e-37 135
Msa0897160 MtrunA17_Chr1g0159581 37.561 205 87 6 231 432 34 200 3.86e-29 113
Msa0897160 MtrunA17_Chr1g0149081 33.523 176 72 2 161 329 1 138 6.70e-22 91.3
Msa0897160 MtrunA17_Chr6g0476081 46.296 108 22 4 327 432 11 84 1.36e-13 66.2
Msa0897160 MtrunA17_Chr6g0450531 43.478 69 36 1 231 296 34 102 3.77e-13 65.9
Msa0897160 MtrunA17_Chr7g0223571 42.718 103 31 3 328 429 1 76 1.02e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0897160 AT5G51740.1 43.340 473 187 15 1 432 1 433 1.10e-109 330
Msa0897160 AT5G51740.2 43.340 473 187 15 1 432 44 476 4.19e-109 330
Msa0897160 AT5G51740.3 42.623 366 165 11 1 327 44 403 2.74e-84 264

Find 72 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGACTGAATTGGGAGATTT+TGG 0.167567 6_1:-88616223 Msa0897160:CDS
CACCGTGTGTTTACAAAATA+TGG 0.176661 6_1:-88616589 Msa0897160:CDS
GAGACTGAGCGGAGTGATTT+AGG 0.187812 6_1:-88616369 Msa0897160:CDS
AATGTTTCCCTTGTTCTATT+TGG 0.195503 6_1:-88616636 Msa0897160:CDS
CCAAAGGTGGTCCTTATAAT+AGG 0.266161 6_1:-88616788 Msa0897160:CDS
ATAGCGAGATCGGGAAATTC+TGG 0.277309 6_1:+88616824 None:intergenic
CTTGTTCTATTTGGAGTTGT+TGG 0.286663 6_1:-88616627 Msa0897160:CDS
GCAACCTTATACGTCGCATT+TGG 0.332750 6_1:-88616247 Msa0897160:CDS
CCATCCAAATGCGACGTATA+AGG 0.337352 6_1:+88616243 None:intergenic
CGCATTTGGATGGACTGAAT+TGG 0.338297 6_1:-88616233 Msa0897160:CDS
TGCCTTCAACTTCAACTTCA+AGG 0.342590 6_1:-88616881 Msa0897160:CDS
CTATTTGGAGTTGTTGGTTC+AGG 0.346705 6_1:-88616621 Msa0897160:CDS
ATGAGAAACTTGATGACAAA+TGG 0.362150 6_1:-88616296 Msa0897160:CDS
CTCTGAGAAAACCTATTATA+AGG 0.371133 6_1:+88616777 None:intergenic
ACACTTTGAGGGTGTGTTGC+CGG 0.386983 6_1:+88616450 None:intergenic
AAGGAAAAGTTGAAGGGAAT+TGG 0.390236 6_1:-88616329 Msa0897160:CDS
AGACTGAGCGGAGTGATTTA+GGG 0.391676 6_1:-88616368 Msa0897160:CDS
TGCCAAGAGGGGCAAAAGAT+TGG 0.400180 6_1:-88616674 Msa0897160:CDS
CTGAGCTTTAGCGAGCGATT+CGG 0.424071 6_1:+88614251 None:intergenic
GATCGGGAAATTCTGGAGTT+AGG 0.430128 6_1:+88616831 None:intergenic
AAACTAGAATCACGTGTGTT+CGG 0.433419 6_1:+88616547 None:intergenic
GCATTTGGATGGACTGAATT+GGG 0.433979 6_1:-88616232 Msa0897160:CDS
ACCCTCAAAGTGTGAGAGTT+GGG 0.436760 6_1:-88616440 Msa0897160:CDS
ACGTGTGTTCGGTTTGTGTA+AGG 0.437622 6_1:+88616558 None:intergenic
CGAAATTAAAATAGCGAGAT+CGG 0.438044 6_1:+88616814 None:intergenic
GAGAATGAAGGAAAAGTTGA+AGG 0.438255 6_1:-88616336 Msa0897160:CDS
TGAATTGCTCTTTCAAGCTC+AGG 0.442207 6_1:-88614286 Msa0897160:CDS
ATCCCAACTCTCACACTTTG+AGG 0.443157 6_1:+88616438 None:intergenic
CCTATTATAAGGACCACCTT+TGG 0.446812 6_1:+88616788 None:intergenic
TTTGGCGACCTTTCGGAGAA+AGG 0.449893 6_1:-88615684 Msa0897160:intron
ACTAAAATGTTCAAGCAAAC+CGG 0.458500 6_1:+88616140 None:intergenic
AAACTGCTCAGCACTGTGTC+TGG 0.465687 6_1:+88615774 None:intergenic
GCTCGCTAAAGCTCAGGTAA+TGG 0.469980 6_1:-88614244 Msa0897160:CDS
TGATGACAAATGGGTTAAGA+AGG 0.473472 6_1:-88616286 Msa0897160:CDS
CCTTATACGTCGCATTTGGA+TGG 0.481840 6_1:-88616243 Msa0897160:CDS
GAGCGGAGTGATTTAGGGTA+TGG 0.481841 6_1:-88616363 Msa0897160:CDS
AAACAAAAGGCATCAACAAA+AGG 0.483071 6_1:+88616189 None:intergenic
GAAATTAAAATAGCGAGATC+GGG 0.493255 6_1:+88616815 None:intergenic
GCTTCAGCTTTAGATATATG+AGG 0.496330 6_1:+88614363 None:intergenic
TCCCAACTCTCACACTTTGA+GGG 0.505571 6_1:+88616439 None:intergenic
TGAGAAACTTGATGACAAAT+GGG 0.508994 6_1:-88616295 Msa0897160:CDS
AGCCTTGAAGTTGAAGTTGA+AGG 0.510117 6_1:+88616879 None:intergenic
GTCTCCAAGCTTCTTCTCCA+TGG 0.512009 6_1:+88616518 None:intergenic
TGATCCTCGTGTGGCTCCTA+CGG 0.513329 6_1:-88614448 Msa0897160:CDS
CGAATCGCTCGCTAAAGCTC+AGG 0.514358 6_1:-88614250 Msa0897160:CDS
TTTAATTTCGCTACCAAAGG+TGG 0.527691 6_1:-88616801 Msa0897160:CDS
CTTCTATTAGTCGCATCAGC+AGG 0.528066 6_1:-88614474 Msa0897160:CDS
AATGCCATGGAGAAGAAGCT+TGG 0.530715 6_1:-88616522 Msa0897160:CDS
GGGTATGGTTCAGAGAATGA+AGG 0.538268 6_1:-88616348 Msa0897160:CDS
TGAAGGGAATTGGCAGATGG+AGG 0.541512 6_1:-88616319 Msa0897160:CDS
CTTCCCACCAGGTAAACAAA+AGG 0.543528 6_1:+88616176 None:intergenic
GGGAAGATAGTTGTATTCAC+CGG 0.553263 6_1:-88616159 Msa0897160:CDS
CAACAACTCCAAATAGAACA+AGG 0.568302 6_1:+88616628 None:intergenic
AGAATGAAGGAAAAGTTGAA+GGG 0.571867 6_1:-88616335 Msa0897160:CDS
CACCCTCAAAGTGTGAGAGT+TGG 0.577769 6_1:-88616441 Msa0897160:CDS
GATTCTAGTTTCGAATGCCA+TGG 0.579969 6_1:-88616535 Msa0897160:CDS
TCTCAGAGAATAGGAACAAG+TGG 0.583572 6_1:-88616762 Msa0897160:CDS
AGTTGAAGGGAATTGGCAGA+TGG 0.592584 6_1:-88616322 Msa0897160:CDS
AATCATTACCTTTCTCCGAA+AGG 0.593435 6_1:+88615676 None:intergenic
ATGAATTTCTCTAAGACCGT+AGG 0.593712 6_1:+88614432 None:intergenic
GATGACAAATGGGTTAAGAA+GGG 0.606043 6_1:-88616285 Msa0897160:CDS
CTGTGTGTTTGAAAAGATGA+AGG 0.609312 6_1:-88616496 Msa0897160:CDS
AGCAGTTTACACATAATCAG+TGG 0.621012 6_1:-88615759 Msa0897160:CDS
TATATCTAAAGCTGAAGCCG+AGG 0.624435 6_1:-88614358 Msa0897160:CDS
CAAATGGGTTAAGAAGGGTA+AGG 0.625766 6_1:-88616280 Msa0897160:CDS
AACAACTCCAAATAGAACAA+GGG 0.652012 6_1:+88616629 None:intergenic
AATACAACTATCTTCCCACC+AGG 0.658440 6_1:+88616165 None:intergenic
AGCAGGCTATGATCCTCGTG+TGG 0.661195 6_1:-88614457 Msa0897160:CDS
GATGAAGAAAACATTGAACA+AGG 0.665144 6_1:-88616391 Msa0897160:CDS
TGTATTGCATGCTCATGCTG+AGG 0.686657 6_1:-88614322 Msa0897160:CDS
CATTGAACAAGGAGACTGAG+CGG 0.761321 6_1:-88616380 Msa0897160:CDS
AAGACCGTAGGAGCCACACG+AGG 0.773543 6_1:+88614444 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAAATTAATAACAATTTT+AGG - chr6_1:88614402-88614421 Msa0897160:CDS 10.0%
!! TTAATAATTCATTGATATAT+AGG - chr6_1:88615532-88615551 Msa0897160:intron 10.0%
!!! ATAGAAAATTGTATAATTTT+AGG - chr6_1:88616519-88616538 Msa0897160:CDS 10.0%
!!! TTTTTAACCAAATTATACTA+AGG - chr6_1:88616081-88616100 Msa0897160:intron 15.0%
!! AGACTATTTGATAGATATTA+TGG - chr6_1:88615132-88615151 Msa0897160:intron 20.0%
!! TTAAGCTATTTATCCAAATA+AGG - chr6_1:88615259-88615278 Msa0897160:intron 20.0%
!! TTGGTTATAAAGATAAACAA+TGG + chr6_1:88615492-88615511 None:intergenic 20.0%
!! AAAAAAGTAAACTTAAAACG+CGG - chr6_1:88615790-88615809 Msa0897160:CDS 20.0%
!! GAATGATTATTAACTGTTAT+AGG - chr6_1:88615859-88615878 Msa0897160:intron 20.0%
!! TAGTTATCCTTAGTATAATT+TGG + chr6_1:88616091-88616110 None:intergenic 20.0%
!! AAATTATACTAAGGATAACT+AGG - chr6_1:88616090-88616109 Msa0897160:intron 20.0%
!! CTAATTTCTAATACTTGATT+TGG + chr6_1:88616143-88616162 None:intergenic 20.0%
!! CAAATCAAGTATTAGAAATT+AGG - chr6_1:88616141-88616160 Msa0897160:CDS 20.0%
!! TAACAAAAGATTACAAACTA+GGG + chr6_1:88616408-88616427 None:intergenic 20.0%
!! ATAACAAAAGATTACAAACT+AGG + chr6_1:88616409-88616428 None:intergenic 20.0%
!!! TGATCTAAAATTATAGGAAT+AGG - chr6_1:88616452-88616471 Msa0897160:CDS 20.0%
! TGAAAAATAGAGTTATGAGA+TGG + chr6_1:88614287-88614306 None:intergenic 25.0%
!!! TATTTTAATTTCGCTACCAA+AGG - chr6_1:88614335-88614354 Msa0897160:CDS 25.0%
!! ATAAAATGCCACACATTTTT+AGG + chr6_1:88614446-88614465 None:intergenic 25.0%
!! TTCCATATTTTGTAAACACA+CGG + chr6_1:88614555-88614574 None:intergenic 25.0%
! GTTATAAAGATAAACAATGG+AGG + chr6_1:88615489-88615508 None:intergenic 25.0%
! GAACATCACAAAAATTCAAT+TGG + chr6_1:88615511-88615530 None:intergenic 25.0%
! GAAGTGAAAAACATGAAATT+AGG - chr6_1:88615611-88615630 Msa0897160:intron 25.0%
!! GGTCAAAGACTAATAAATTT+TGG - chr6_1:88615632-88615651 Msa0897160:intron 25.0%
! ATAATCATTCATTTATGCGA+AGG + chr6_1:88615849-88615868 None:intergenic 25.0%
! TAGGAATGATTAAATGTTCT+CGG - chr6_1:88615878-88615897 Msa0897160:intron 25.0%
!! AACCAACTTTTATAGAGATT+TGG - chr6_1:88616021-88616040 Msa0897160:intron 25.0%
!!! TTCTAATACTTGATTTGGTT+CGG + chr6_1:88616138-88616157 None:intergenic 25.0%
!! ATAATTTTAGATCATGTCAC+AGG + chr6_1:88616446-88616465 None:intergenic 25.0%
!! GTGACATGATCTAAAATTAT+AGG - chr6_1:88616446-88616465 Msa0897160:CDS 25.0%
!! TGTATAATTTTAGGCGTTTT+AGG - chr6_1:88616528-88616547 Msa0897160:CDS 25.0%
! AAAATTTATTGACGACATTC+AGG - chr6_1:88616615-88616634 Msa0897160:CDS 25.0%
! AAAAAGAAGCTGATTACATT+GGG - chr6_1:88616642-88616661 Msa0897160:CDS 25.0%
GAAATTAAAATAGCGAGATC+GGG + chr6_1:88614327-88614346 None:intergenic 30.0%
CGAAATTAAAATAGCGAGAT+CGG + chr6_1:88614328-88614347 None:intergenic 30.0%
! CTCTGAGAAAACCTATTATA+AGG + chr6_1:88614365-88614384 None:intergenic 30.0%
! AATAGGTTTTCTCAGAGAAT+AGG - chr6_1:88614368-88614387 Msa0897160:CDS 30.0%
! AGAACAAGGGAAACATTTTT+AGG + chr6_1:88614500-88614519 None:intergenic 30.0%
AATGTTTCCCTTGTTCTATT+TGG - chr6_1:88614503-88614522 Msa0897160:CDS 30.0%
AACAACTCCAAATAGAACAA+GGG + chr6_1:88614513-88614532 None:intergenic 30.0%
! CTTTAAAAATGAGTTGTTGC+CGG - chr6_1:88614670-88614689 Msa0897160:intron 30.0%
GATGAAGAAAACATTGAACA+AGG - chr6_1:88614748-88614767 Msa0897160:intron 30.0%
AGAATGAAGGAAAAGTTGAA+GGG - chr6_1:88614804-88614823 Msa0897160:intron 30.0%
! ATGAGAAACTTGATGACAAA+TGG - chr6_1:88614843-88614862 Msa0897160:intron 30.0%
! TGAGAAACTTGATGACAAAT+GGG - chr6_1:88614844-88614863 Msa0897160:intron 30.0%
!! AAACAAAAGGCATCAACAAA+AGG + chr6_1:88614953-88614972 None:intergenic 30.0%
ACTAAAATGTTCAAGCAAAC+CGG + chr6_1:88615002-88615021 None:intergenic 30.0%
GCAAAAACAATGACCTTATT+TGG + chr6_1:88615275-88615294 None:intergenic 30.0%
AAATCTAACACTTTACTGCT+TGG - chr6_1:88615319-88615338 Msa0897160:intron 30.0%
! ATAGGTAATGTTTGTTAGAC+AGG - chr6_1:88615550-88615569 Msa0897160:intron 30.0%
TTTGTTAGACAGGTTAGAAT+AGG - chr6_1:88615560-88615579 Msa0897160:intron 30.0%
CAACCCTTGATTTAAAGAAA+GGG + chr6_1:88615816-88615835 None:intergenic 30.0%
!! ATTATTCTAGAGGTACTACT+AGG + chr6_1:88615922-88615941 None:intergenic 30.0%
!! CCTCTAGAATAATTATCGTT+CGG - chr6_1:88615929-88615948 Msa0897160:intron 30.0%
! AGTTCAATTTTTCTGAACTG+TGG + chr6_1:88615979-88615998 None:intergenic 30.0%
CACCAAATCTCTATAAAAGT+TGG + chr6_1:88616026-88616045 None:intergenic 30.0%
TGGTTTAGAAACTACAGAAA+TGG + chr6_1:88616051-88616070 None:intergenic 30.0%
TGTAGTTTCTAAACCAAACT+GGG - chr6_1:88616055-88616074 Msa0897160:intron 30.0%
TGGTTAAAAAAATCCCAGTT+TGG + chr6_1:88616071-88616090 None:intergenic 30.0%
TAAATTGAACTAAACCGAAC+TGG - chr6_1:88616219-88616238 Msa0897160:CDS 30.0%
CTATATCAACCAAGAAATGT+GGG + chr6_1:88616503-88616522 None:intergenic 30.0%
TCTATATCAACCAAGAAATG+TGG + chr6_1:88616504-88616523 None:intergenic 30.0%
! TTAGGATGCAATATTGATTG+TGG - chr6_1:88616546-88616565 Msa0897160:CDS 30.0%
! ATGCAATATTGATTGTGGAA+AGG - chr6_1:88616551-88616570 Msa0897160:CDS 30.0%
GAAAAAGAAGCTGATTACAT+TGG - chr6_1:88616641-88616660 Msa0897160:CDS 30.0%
!! TACAGCTTTATATTTTGCCT+CGG + chr6_1:88616801-88616820 None:intergenic 30.0%
! TTTAATTTCGCTACCAAAGG+TGG - chr6_1:88614338-88614357 Msa0897160:CDS 35.0%
CTGTGTATCCTAAAAATGTG+TGG - chr6_1:88614435-88614454 Msa0897160:CDS 35.0%
CAACAACTCCAAATAGAACA+AGG + chr6_1:88614514-88614533 None:intergenic 35.0%
! CTTGTTCTATTTGGAGTTGT+TGG - chr6_1:88614512-88614531 Msa0897160:intron 35.0%
CACCGTGTGTTTACAAAATA+TGG - chr6_1:88614550-88614569 Msa0897160:intron 35.0%
AAACTAGAATCACGTGTGTT+CGG + chr6_1:88614595-88614614 None:intergenic 35.0%
CTGTGTGTTTGAAAAGATGA+AGG - chr6_1:88614643-88614662 Msa0897160:intron 35.0%
GAGAATGAAGGAAAAGTTGA+AGG - chr6_1:88614803-88614822 Msa0897160:intron 35.0%
AAGGAAAAGTTGAAGGGAAT+TGG - chr6_1:88614810-88614829 Msa0897160:intron 35.0%
!! TGATGACAAATGGGTTAAGA+AGG - chr6_1:88614853-88614872 Msa0897160:intron 35.0%
!! GATGACAAATGGGTTAAGAA+GGG - chr6_1:88614854-88614873 Msa0897160:intron 35.0%
! GCAGAGATAGCAACTTTTAT+CGG - chr6_1:88615028-88615047 Msa0897160:intron 35.0%
ATTCGCTCTTTATGTCTGTT+TGG - chr6_1:88615069-88615088 Msa0897160:intron 35.0%
! CACTTTACTGCTTGGTAATT+AGG - chr6_1:88615327-88615346 Msa0897160:intron 35.0%
! TTACTGCTTGGTAATTAGGT+TGG - chr6_1:88615331-88615350 Msa0897160:intron 35.0%
AGCAGTTTACACATAATCAG+TGG - chr6_1:88615380-88615399 Msa0897160:intron 35.0%
!! GACTGTCATCACTTTTCTTT+TGG - chr6_1:88615437-88615456 Msa0897160:intron 35.0%
AATCATTACCTTTCTCCGAA+AGG + chr6_1:88615466-88615485 None:intergenic 35.0%
GCAACCCTTGATTTAAAGAA+AGG + chr6_1:88615817-88615836 None:intergenic 35.0%
CCGAACGATAATTATTCTAG+AGG + chr6_1:88615932-88615951 None:intergenic 35.0%
GAATAATTATCGTTCGGTTC+AGG - chr6_1:88615935-88615954 Msa0897160:intron 35.0%
!! TCTCTATAAAAGTTGGTTCG+AGG + chr6_1:88616019-88616038 None:intergenic 35.0%
CTGTAGTTTCTAAACCAAAC+TGG - chr6_1:88616054-88616073 Msa0897160:intron 35.0%
TGAACTATACGAAACCAGTT+CGG + chr6_1:88616236-88616255 None:intergenic 35.0%
GTAATTAAGCCCACATTTCT+TGG - chr6_1:88616491-88616510 Msa0897160:CDS 35.0%
! TATTGATTGTGGAAAGGTGA+AGG - chr6_1:88616557-88616576 Msa0897160:CDS 35.0%
TTTATTGACGACATTCAGGA+TGG - chr6_1:88616619-88616638 Msa0897160:CDS 35.0%
!! ATGAATTTCTCTAAGACCGT+AGG + chr6_1:88616710-88616729 None:intergenic 35.0%
!! GCTTCAGCTTTAGATATATG+AGG + chr6_1:88616779-88616798 None:intergenic 35.0%
TGCCTTCAACTTCAACTTCA+AGG - chr6_1:88614258-88614277 Msa0897160:CDS 40.0%
AGCCTTGAAGTTGAAGTTGA+AGG + chr6_1:88614263-88614282 None:intergenic 40.0%
CCTATTATAAGGACCACCTT+TGG + chr6_1:88614354-88614373 None:intergenic 40.0%
CCAAAGGTGGTCCTTATAAT+AGG - chr6_1:88614351-88614370 Msa0897160:CDS 40.0%
TCTCAGAGAATAGGAACAAG+TGG - chr6_1:88614377-88614396 Msa0897160:CDS 40.0%
! TGTGGCATTTTATGCCAAGA+GGG - chr6_1:88614453-88614472 Msa0897160:CDS 40.0%
!! CTATTTGGAGTTGTTGGTTC+AGG - chr6_1:88614518-88614537 Msa0897160:intron 40.0%
!! GATTCTAGTTTCGAATGCCA+TGG - chr6_1:88614604-88614623 Msa0897160:intron 40.0%
!! CAAATGGGTTAAGAAGGGTA+AGG - chr6_1:88614859-88614878 Msa0897160:intron 40.0%
!! GCATTTGGATGGACTGAATT+GGG - chr6_1:88614907-88614926 Msa0897160:intron 40.0%
! TGGACTGAATTGGGAGATTT+TGG - chr6_1:88614916-88614935 Msa0897160:intron 40.0%
! ACTGAATTGGGAGATTTTGG+TGG - chr6_1:88614919-88614938 Msa0897160:intron 40.0%
!! GTTGATGCCTTTTGTTTACC+TGG - chr6_1:88614956-88614975 Msa0897160:intron 40.0%
!! ATGCCTTTTGTTTACCTGGT+GGG - chr6_1:88614960-88614979 Msa0897160:intron 40.0%
AATACAACTATCTTCCCACC+AGG + chr6_1:88614977-88614996 None:intergenic 40.0%
GGGAAGATAGTTGTATTCAC+CGG - chr6_1:88614980-88614999 Msa0897160:intron 40.0%
! CTTTTCTTTTGGCGACCTTT+CGG - chr6_1:88615448-88615467 Msa0897160:intron 40.0%
TTGGCTCAATTAGTGTTACC+AGG - chr6_1:88615651-88615670 Msa0897160:intron 40.0%
!! CTTGCGAGTCAACTTGTTTT+GGG + chr6_1:88615698-88615717 None:intergenic 40.0%
CGGCCCTTTCTTTAAATCAA+GGG - chr6_1:88615810-88615829 Msa0897160:intron 40.0%
TCTGAACTGTGGTAACAGTT+CGG + chr6_1:88615968-88615987 None:intergenic 40.0%
TGCAGTTCAGTTCAGCAATT+TGG + chr6_1:88616188-88616207 None:intergenic 40.0%
CTAGTTCATAACAATGCTGC+AGG + chr6_1:88616349-88616368 None:intergenic 40.0%
AACTAGTGAAACGTATGAGC+TGG - chr6_1:88616363-88616382 Msa0897160:CDS 40.0%
TGTGGGCTTAATTACACAAG+TGG + chr6_1:88616486-88616505 None:intergenic 40.0%
! TATATCTAAAGCTGAAGCCG+AGG - chr6_1:88616781-88616800 Msa0897160:CDS 40.0%
TGAATTGCTCTTTCAAGCTC+AGG - chr6_1:88616853-88616872 Msa0897160:CDS 40.0%
!! GATCGGGAAATTCTGGAGTT+AGG + chr6_1:88614311-88614330 None:intergenic 45.0%
ATAGCGAGATCGGGAAATTC+TGG + chr6_1:88614318-88614337 None:intergenic 45.0%
! GTGTGGCATTTTATGCCAAG+AGG - chr6_1:88614452-88614471 Msa0897160:CDS 45.0%
! GTGGCATTTTATGCCAAGAG+GGG - chr6_1:88614454-88614473 Msa0897160:CDS 45.0%
! AACCAATCTTTTGCCCCTCT+TGG + chr6_1:88614470-88614489 None:intergenic 45.0%
! ACGTGTGTTCGGTTTGTGTA+AGG + chr6_1:88614584-88614603 None:intergenic 45.0%
AATGCCATGGAGAAGAAGCT+TGG - chr6_1:88614617-88614636 Msa0897160:intron 45.0%
ACCCTCAAAGTGTGAGAGTT+GGG - chr6_1:88614699-88614718 Msa0897160:intron 45.0%
TCCCAACTCTCACACTTTGA+GGG + chr6_1:88614703-88614722 None:intergenic 45.0%
ATCCCAACTCTCACACTTTG+AGG + chr6_1:88614704-88614723 None:intergenic 45.0%
CATTGAACAAGGAGACTGAG+CGG - chr6_1:88614759-88614778 Msa0897160:intron 45.0%
AGACTGAGCGGAGTGATTTA+GGG - chr6_1:88614771-88614790 Msa0897160:intron 45.0%
GGGTATGGTTCAGAGAATGA+AGG - chr6_1:88614791-88614810 Msa0897160:intron 45.0%
AGTTGAAGGGAATTGGCAGA+TGG - chr6_1:88614817-88614836 Msa0897160:intron 45.0%
GCAACCTTATACGTCGCATT+TGG - chr6_1:88614892-88614911 Msa0897160:intron 45.0%
CCATCCAAATGCGACGTATA+AGG + chr6_1:88614899-88614918 None:intergenic 45.0%
CCTTATACGTCGCATTTGGA+TGG - chr6_1:88614896-88614915 Msa0897160:intron 45.0%
!! CGCATTTGGATGGACTGAAT+TGG - chr6_1:88614906-88614925 Msa0897160:intron 45.0%
!! GATGCCTTTTGTTTACCTGG+TGG - chr6_1:88614959-88614978 Msa0897160:intron 45.0%
CTTCCCACCAGGTAAACAAA+AGG + chr6_1:88614966-88614985 None:intergenic 45.0%
! AGCAACTTTTATCGGACGTG+AGG - chr6_1:88615036-88615055 Msa0897160:intron 45.0%
GGAGGCCAAAATCGTAAACT+CGG + chr6_1:88615677-88615696 None:intergenic 45.0%
!! GCTTGCGAGTCAACTTGTTT+TGG + chr6_1:88615699-88615718 None:intergenic 45.0%
!!! GCGAGTCTTTTGAGTTTGTG+CGG + chr6_1:88615733-88615752 None:intergenic 45.0%
GCGGCCCTTTCTTTAAATCA+AGG - chr6_1:88615809-88615828 Msa0897160:intron 45.0%
GGTTCGAGGCAATTCGATAT+GGG + chr6_1:88616005-88616024 None:intergenic 45.0%
TGGTTCGAGGCAATTCGATA+TGG + chr6_1:88616006-88616025 None:intergenic 45.0%
GATTGTGGAAAGGTGAAGGA+CGG - chr6_1:88616561-88616580 Msa0897160:CDS 45.0%
! GGTGAAGGACGGTAATTTGT+TGG - chr6_1:88616572-88616591 Msa0897160:CDS 45.0%
CTTCTATTAGTCGCATCAGC+AGG - chr6_1:88616665-88616684 Msa0897160:CDS 45.0%
TGTATTGCATGCTCATGCTG+AGG - chr6_1:88616817-88616836 Msa0897160:CDS 45.0%
TGCCAAGAGGGGCAAAAGAT+TGG - chr6_1:88614465-88614484 Msa0897160:CDS 50.0%
GTCTCCAAGCTTCTTCTCCA+TGG + chr6_1:88614624-88614643 None:intergenic 50.0%
! ACACTTTGAGGGTGTGTTGC+CGG + chr6_1:88614692-88614711 None:intergenic 50.0%
CACCCTCAAAGTGTGAGAGT+TGG - chr6_1:88614698-88614717 Msa0897160:intron 50.0%
GAGACTGAGCGGAGTGATTT+AGG - chr6_1:88614770-88614789 Msa0897160:intron 50.0%
! GAGCGGAGTGATTTAGGGTA+TGG - chr6_1:88614776-88614795 Msa0897160:intron 50.0%
TGAAGGGAATTGGCAGATGG+AGG - chr6_1:88614820-88614839 Msa0897160:intron 50.0%
AAACTGCTCAGCACTGTGTC+TGG + chr6_1:88615368-88615387 None:intergenic 50.0%
TTTGGCGACCTTTCGGAGAA+AGG - chr6_1:88615455-88615474 Msa0897160:intron 50.0%
CCAAAATCGTAAACTCGGCC+TGG + chr6_1:88615672-88615691 None:intergenic 50.0%
! CCAGGCCGAGTTTACGATTT+TGG - chr6_1:88615669-88615688 Msa0897160:intron 50.0%
!!! GCGAGTCAACTTGTTTTGGG+AGG + chr6_1:88615695-88615714 None:intergenic 50.0%
GTGATACGCCCAATCCTCAT+CGG - chr6_1:88616297-88616316 Msa0897160:CDS 50.0%
TGATACGCCCAATCCTCATC+GGG - chr6_1:88616298-88616317 Msa0897160:CDS 50.0%
! CTGTAACAATGCCGCTGGTT+TGG + chr6_1:88616324-88616343 None:intergenic 50.0%
AGGTTCTGTAACAATGCCGC+TGG + chr6_1:88616329-88616348 None:intergenic 50.0%
! CTGAGCTTTAGCGAGCGATT+CGG + chr6_1:88616891-88616910 None:intergenic 50.0%
GCTCGCTAAAGCTCAGGTAA+TGG - chr6_1:88616895-88616914 Msa0897160:CDS 50.0%
GGTTTGGCCCGATGAGGATT+GGG + chr6_1:88616308-88616327 None:intergenic 55.0%
! TGGTTTGGCCCGATGAGGAT+TGG + chr6_1:88616309-88616328 None:intergenic 55.0%
AGCAGGCTATGATCCTCGTG+TGG - chr6_1:88616682-88616701 Msa0897160:CDS 55.0%
TGATCCTCGTGTGGCTCCTA+CGG - chr6_1:88616691-88616710 Msa0897160:CDS 55.0%
CGAATCGCTCGCTAAAGCTC+AGG - chr6_1:88616889-88616908 Msa0897160:CDS 55.0%
TCCTCATCGGGCCAAACCAG+CGG - chr6_1:88616310-88616329 Msa0897160:CDS 60.0%
AAGACCGTAGGAGCCACACG+AGG + chr6_1:88616698-88616717 None:intergenic 60.0%
!! GCCGCTGGTTTGGCCCGATG+AGG + chr6_1:88616314-88616333 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 88614206 88616955 88614206 ID=Msa0897160;Name=Msa0897160
chr6_1 mRNA 88614206 88616955 88614206 ID=Msa0897160-mRNA-1;Parent=Msa0897160;Name=Msa0897160-mRNA-1;_AED=0.12;_eAED=0.13;_QI=0|0.5|0.33|0.66|1|1|3|0|432
chr6_1 exon 88616104 88616955 88616104 ID=Msa0897160-mRNA-1:exon:15020;Parent=Msa0897160-mRNA-1
chr6_1 exon 88615685 88615812 88615685 ID=Msa0897160-mRNA-1:exon:15019;Parent=Msa0897160-mRNA-1
chr6_1 exon 88614206 88614524 88614206 ID=Msa0897160-mRNA-1:exon:15018;Parent=Msa0897160-mRNA-1
chr6_1 CDS 88616104 88616955 88616104 ID=Msa0897160-mRNA-1:cds;Parent=Msa0897160-mRNA-1
chr6_1 CDS 88615685 88615812 88615685 ID=Msa0897160-mRNA-1:cds;Parent=Msa0897160-mRNA-1
chr6_1 CDS 88614206 88614524 88614206 ID=Msa0897160-mRNA-1:cds;Parent=Msa0897160-mRNA-1
Gene Sequence

>Msa0897160

ATGGCTTGGTTTAGAAGAACTGAAAAGCTTATTGTTCAAGCTTTTCGTGCATTGCCTTCAACTTCAACTTCAAGGCTTCCATCTCATAACTCTATTTTTCAGCCTAACTCCAGAATTTCCCGATCTCGCTATTTTAATTTCGCTACCAAAGGTGGTCCTTATAATAGGTTTTCTCAGAGAATAGGAACAAGTGGTTTTGAAATTAATAACAATTTTAGGAGATTTTACTCTGTGTATCCTAAAAATGTGTGGCATTTTATGCCAAGAGGGGCAAAAGATTGGTTTCAAGATCCTAAAAATGTTTCCCTTGTTCTATTTGGAGTTGTTGGTTCAGGTGTTTTAATCACCGTGTGTTTACAAAATATGGAAACTATTCCTTACACAAACCGAACACACGTGATTCTAGTTTCGAATGCCATGGAGAAGAAGCTTGGAGACTGTGTGTTTGAAAAGATGAAGGCAGACTTTAAAAATGAGTTGTTGCCGGCAACACACCCTCAAAGTGTGAGAGTTGGGATGATAGCAAACAATATTCTCGATGCGATGAAGAAAACATTGAACAAGGAGACTGAGCGGAGTGATTTAGGGTATGGTTCAGAGAATGAAGGAAAAGTTGAAGGGAATTGGCAGATGGAGGATGAGAAACTTGATGACAAATGGGTTAAGAAGGGTAAGGAACAAGATTCGCAACCTTATACGTCGCATTTGGATGGACTGAATTGGGAGATTTTGGTGGTGAATAAGCCTTTTGTTGATGCCTTTTGTTTACCTGGTGGGAAGATAGTTGTATTCACCGGTTTGCTTGAACATTTTAGTAGTGATGCAGAGATAGCAACTTTTATCGGACGTGAGGTTGGACATGTTGTAGCCAGACACAGTGCTGAGCAGTTTACACATAATCAGTGGTTTTCAGAACTTAGACATAGTAGTGATAATAACAGACTGTCATCACTTTTCTTTTGGCGACCTTTCGGAGAAAGGATGGAAAAAGAAGCTGATTACATTGGGCTTCTATTAGTCGCATCAGCAGGCTATGATCCTCGTGTGGCTCCTACGGTCTTAGAGAAATTCATTGATCGTGATTCTATGCTTACAGATTATCGCTCTACTCAACATCGACCTCATATATCTAAAGCTGAAGCCGAGGCAAAATATAAAGCTGTATTGCATGCTCATGCTGAGGAAAAAAAAAGAGCTGAATTGCTCTTTCAAGCTCAGGTAAGAAAAAGAGCCGAATCGCTCGCTAAAGCTCAGGTAATGGATGAAGCTCTTACTATATATGAGAATGTAAGAGCTTGA
Protein sequence

>Msa0897160

MAWFRRTEKLIVQAFRALPSTSTSRLPSHNSIFQPNSRISRSRYFNFATKGGPYNRFSQRIGTSGFEINNNFRRFYSVYPKNVWHFMPRGAKDWFQDPKNVSLVLFGVVGSGVLITVCLQNMETIPYTNRTHVILVSNAMEKKLGDCVFEKMKADFKNELLPATHPQSVRVGMIANNILDAMKKTLNKETERSDLGYGSENEGKVEGNWQMEDEKLDDKWVKKGKEQDSQPYTSHLDGLNWEILVVNKPFVDAFCLPGGKIVVFTGLLEHFSSDAEIATFIGREVGHVVARHSAEQFTHNQWFSELRHSSDNNRLSSLFFWRPFGERMEKEADYIGLLLVASAGYDPRVAPTVLEKFIDRDSMLTDYRSTQHRPHISKAEAEAKYKAVLHAHAEEKKRAELLFQAQVRKRAESLAKAQVMDEALTIYENVRA*