AlfalfaGEDB | Msa0912580

M. sativa cultivar zhongmu-4 / Msa0912580

KEGG: -
GO: GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
PFAM: PF12799.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0912580	XP_024641548.1	79.828	699	131	1	9	697	209	907	0.0	1114

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0912580	sp\|Q6JN46\|EIX2_SOLLC	40.974	698	368	14	9	696	346	1009	1.53e-144	451
Msa0912580	sp\|Q6JN46\|EIX2_SOLLC	25.160	469	244	15	210	597	132	574	4.18e-13	76.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014790	Msa0912580	0.808786	2.609705e-50	-8.615850e-47
Msa0063100	Msa0912580	0.810963	8.876655e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0912580	MtrunA17_Chr6g0465151	79.828	699	131	1	9	697	209	907	0.0	1114
Msa0912580	MtrunA17_Chr6g0465151	28.446	341	193	10	292	610	54	365	3.93e-12	69.7
Msa0912580	MtrunA17_Chr6g0465181	82.200	691	107	3	16	696	459	1143	0.0	1099
Msa0912580	MtrunA17_Chr6g0465181	27.955	533	288	24	99	597	119	589	1.28e-12	71.6
Msa0912580	MtrunA17_Chr6g0465291	78.827	699	138	1	9	697	505	1203	0.0	1097
Msa0912580	MtrunA17_Chr6g0465291	38.849	139	83	2	326	463	123	260	7.91e-16	82.0
Msa0912580	MtrunA17_Chr6g0465291	26.677	656	387	26	9	598	163	790	4.78e-15	79.3
Msa0912580	MtrunA17_Chr6g0465291	28.646	384	187	14	271	599	139	490	1.50e-13	74.7
Msa0912580	MtrunA17_Chr6g0465291	28.966	435	246	18	206	606	149	554	2.61e-13	73.9
Msa0912580	MtrunA17_Chr6g0465291	26.280	586	275	24	99	602	137	647	7.54e-13	72.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0912580	AT2G34930.1	33.431	682	363	14	9	628	250	902	6.19e-90	300
Msa0912580	AT2G34930.1	33.119	311	150	11	316	600	114	392	1.20e-18	91.3
Msa0912580	AT2G34930.1	26.429	420	222	12	5	410	544	890	4.25e-18	89.4
Msa0912580	AT2G34930.1	26.148	501	318	16	139	614	82	555	3.76e-16	83.2
Msa0912580	AT4G04220.1	31.751	674	393	18	11	632	110	768	1.56e-68	240
Msa0912580	AT4G04220.1	30.310	419	225	14	233	617	88	473	2.50e-25	112
Msa0912580	AT2G32680.1	30.202	692	394	19	11	632	152	824	1.00e-66	236
Msa0912580	AT2G32680.1	30.030	333	173	9	320	598	104	430	3.66e-18	89.7
Msa0912580	AT2G33060.1	31.517	679	386	22	9	664	125	747	2.86e-66	234
Msa0912580	AT2G33060.1	28.329	413	190	14	239	598	92	451	2.08e-16	84.0

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Find 122 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTGCAAGCATTACTATTG+AGG	0.482917	6_2:-21576932	Msa0912580:CDS
AAGATTATCTTCGTAACTTG+TGG	0.536246	6_2:+21576197	None:intergenic
AATGAGAATATTGAGAGATC+AGG	0.485114	6_2:+21577680	None:intergenic
AATGCATTCTTGACATGGAA+AGG	0.590497	6_2:-21576549	Msa0912580:CDS
AATGGCAAGCTACCACCGTC+AGG	0.520737	6_2:+21577913	None:intergenic
AATGGTATAGATGCAATGTT+AGG	0.474510	6_2:-21577092	Msa0912580:CDS
AATTCACTTCCAATCCAATA+AGG	0.245192	6_2:+21576813	None:intergenic
AATTCCTAAATCATATATTT+TGG	0.149552	6_2:-21577845	Msa0912580:CDS
AATTGAATGCATTCTTGACA+TGG	0.572667	6_2:-21576554	Msa0912580:CDS
AATTGCAGATTTGATTCAAT+TGG	0.402521	6_2:-21576436	Msa0912580:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTTAGATCTCAGTACTAA+TGG	-	chr6_2:21575975-21575994	Msa0912580:CDS	30.0%
TGATGTGAATATAGAAAGGT+CGG	+	chr6_2:21576018-21576037	None:intergenic	30.0%
GATAAATTCTCCAACTGAAA+TGG	+	chr6_2:21576104-21576123	None:intergenic	30.0%
ATCACATCAAACTCTTTAGA+AGG	-	chr6_2:21576125-21576144	Msa0912580:CDS	30.0%
TAGCACATCTAGATAATTGA+TGG	+	chr6_2:21576243-21576262	None:intergenic	30.0%
AATGAGAATATTGAGAGATC+AGG	+	chr6_2:21576319-21576338	None:intergenic	30.0%
ATAAGTTCGAACTCTTTAAG+TGG	-	chr6_2:21576427-21576446	Msa0912580:CDS	30.0%
ACATTGACATACCAGAAAAA+TGG	+	chr6_2:21576468-21576487	None:intergenic	30.0%
TTCTGGTATGTCAATGTTAA+AGG	-	chr6_2:21576471-21576490	Msa0912580:CDS	30.0%
TGAATCCATTTAGGAAATGT+AGG	+	chr6_2:21576598-21576617	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	21575948	21578070	21575948	ID=Msa0912580;Name=Msa0912580
chr6_2	mRNA	21575948	21578070	21575948	ID=Msa0912580-mRNA-1;Parent=Msa0912580;Name=Msa0912580-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|0\|0.5\|2\|0\|697
chr6_2	exon	21577835	21578070	21577835	ID=Msa0912580-mRNA-1:exon:4297;Parent=Msa0912580-mRNA-1
chr6_2	exon	21575948	21577805	21575948	ID=Msa0912580-mRNA-1:exon:4296;Parent=Msa0912580-mRNA-1
chr6_2	CDS	21577835	21578070	21577835	ID=Msa0912580-mRNA-1:cds;Parent=Msa0912580-mRNA-1
chr6_2	CDS	21575948	21577805	21575948	ID=Msa0912580-mRNA-1:cds;Parent=Msa0912580-mRNA-1

Gene Sequence

>Msa0912580

CGGACCAGCTTGTTTGTTCAAAAGAGGTTGTTAGATCTCAGTACTAATGGAATCACTGGAACACTACCCGACCTTTCTATATTCACATCATTGAAAACATTGGATTTTTCAGGTAATAAGTTGAGTGGAAAGATTCCTGACGGTGGTAGCTTGCCATTTCAGTTGGAGAATTTATCCATCACATCAAACTCTTTAGAAGGGGTAATTCCTAAATCATATATTTTGGATGAATGCATATTCAATGGTGAGCTTCAAGTAATAATCCATCAATTATCTAGATGTGCTAGATACTCACTTCAACAATTAGATCTAAGTAGTAATCAAATCAATGGCACCCTACCTGATCTCTCAATATTCTCATTTTTGGAAATATTTGATATTTCTGAAAATAGGCTAAACGGGAAGATATTTGAAGATATTCGATTTCCAACTACACTAAGGACACTTGAAATAAGTTCGAACTCTTTAAGTGGTGTGATCTTTGATTTCCATTTTTCTGGTATGTCAATGTTAAAGGATTTAGACTTGTCAGACAATTTACTAGCATTGAGATTTACTGAAAATTGGGTCCCGCCTTTTCAACTGCACAATATAGGATTGAGATCATGCAAACTTGGTCCTACATTTCCTAAATGGATTCAGACACAAAAATACCTGCAGGATCTTGATATTTCGAATGCTGGAATTTCAGATAATGTTCCAGAGGGGTTTTGGGCTATACTCTCATTACAAGAGTGCTTGAAAATCAACATTTCAAACAATAATCTCAAAGGATCAATTCCAAATCTTCAAGTCAAAAATCACTGTTCATATTTATATTTTTCATCAAATGGATTTGAAGGGCCCATTCCACCGTTTCTCCAAGGTTCCATGGTTATCGATATGTCCAAAAATAAATTCTCTGATTGTCTTCCATTCTTATGTGCAAATGGTATAGATGCAATGTTAGGCCAGTTTGACCTTTCAAATAATCAATTATCTGGACGAATTCCAGACTGTTGGAGCAATTTCAAGGCATTAGCTTATCTAGATTTGAGCCACAACAATTTTTCAGGGAAGATTCCTACTTCAATGGGATCACTTGTTAAACTGCAAGCATTACTATTGAGGAACAACAGCTTAACAGAGGAAATGCCTTTCTCCTTAATGAACTGCACACAGTTAGTAATGCTTGATTTGCGAGAAAACAGATTAGAAGGACTCGTCCCTTATTGGATTGGAAGTGAATTAAAAGAGCTGCAAGTTTTAAGCTTACAAAGGAATCAATTCTTTGGAAGTTTACCATTGGAACTCTGCTATCTACAAAGCATTCAACTTTTTGATCTCTCATTAAACAATCTATCTGGACGAATTCCCAAATGCATAAAAAATCTCACTTCAATGACTCAAAAAGCTTCATTACATAATTTTACCAATCATTATTATGTAATAAGCCAAGGATATGTGGGTTATGGACAACAATATGAATTGAATGCATTCTTGACATGGAAAGGTGTAGAACATGTGTTCAACAACAATGGATTGGTTCTTCTAAAAGTCATACATCTCTCAAGCAATCATTTTTCAAAAGAGATTCCACATGAAATTGCAGATTTGATTCAATTGGTCTCATTGAATTTATCAAGAAACAACTTTAATGGGAAATTTCCTTCAAATATTGGAAAGCTAACATCACTTGACTTTCTTGATTTATCACGAAATAAGCTGATTGGTTTAATTCCTTCTAGTCTCTCCCAAATAGAACCGCTTGGTGTGTTAGATTTGTCACATAATCAATTATCTGGAGAGATTCCAACGAGTACACAACTACAAAGTTTCAACGCCACAAGTTACGAAGATAATCTTGATCTTTGTGGTCCACCACTTGTAAAATTATGTACTCAGGGGGAGCCGCCACATGATCCAAAAGTAGAAGTTCAAGACGATGAAGATTTGCTTCTCAATCGTGGATTTTACATCAGTCCGATATTCAGATTTATTATAGGCTTCTGGGGAGTATTTAGTTCAATCCTAATCAAGCGTTCTTGGAGACATGCTTATTTCAAGTTCATCAATAATTTAGTGGATAATATTTACGTAAAATGCAGATGGTGA

Protein sequence

>Msa0912580

RTSLFVQKRLLDLSTNGITGTLPDLSIFTSLKTLDFSGNKLSGKIPDGGSLPFQLENLSITSNSLEGVIPKSYILDECIFNGELQVIIHQLSRCARYSLQQLDLSSNQINGTLPDLSIFSFLEIFDISENRLNGKIFEDIRFPTTLRTLEISSNSLSGVIFDFHFSGMSMLKDLDLSDNLLALRFTENWVPPFQLHNIGLRSCKLGPTFPKWIQTQKYLQDLDISNAGISDNVPEGFWAILSLQECLKINISNNNLKGSIPNLQVKNHCSYLYFSSNGFEGPIPPFLQGSMVIDMSKNKFSDCLPFLCANGIDAMLGQFDLSNNQLSGRIPDCWSNFKALAYLDLSHNNFSGKIPTSMGSLVKLQALLLRNNSLTEEMPFSLMNCTQLVMLDLRENRLEGLVPYWIGSELKELQVLSLQRNQFFGSLPLELCYLQSIQLFDLSLNNLSGRIPKCIKNLTSMTQKASLHNFTNHYYVISQGYVGYGQQYELNAFLTWKGVEHVFNNNGLVLLKVIHLSSNHFSKEIPHEIADLIQLVSLNLSRNNFNGKFPSNIGKLTSLDFLDLSRNKLIGLIPSSLSQIEPLGVLDLSHNQLSGEIPTSTQLQSFNATSYEDNLDLCGPPLVKLCTQGEPPHDPKVEVQDDEDLLLNRGFYISPIFRFIIGFWGVFSSILIKRSWRHAYFKFINNLVDNIYVKCRW*