AlfalfaGEDB | Msa0923130

M. sativa cultivar zhongmu-4 / Msa0923130

KEGG: K04733
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0923130	XP_004487573.1	87.678	211	26	0	186	396	59	269	9.70e-130	392

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0923130	sp\|F4JEQ2\|PBL23_ARATH	47.059	221	103	3	186	396	41	257	4.21e-58	199

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0923130	Msa0965710	0.924738	4.715806e-90	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0923130	MtrunA17_Chr2g0320111	91.943	211	17	0	186	396	60	270	4.19e-131	385
Msa0923130	MtrunA17_Chr1g0191631	51.777	197	89	2	201	396	133	324	7.86e-67	221
Msa0923130	MtrunA17_Chr1g0213991	54.082	196	80	3	204	396	83	271	1.01e-59	203
Msa0923130	MtrunA17_Chr7g0237401	52.577	194	86	2	204	396	79	267	2.21e-58	199
Msa0923130	MtrunA17_Chr8g0364671	49.239	197	91	2	202	396	47	236	8.41e-58	195
Msa0923130	MtrunA17_Chr5g0413981	48.500	200	91	3	202	396	47	239	3.18e-57	194
Msa0923130	MtrunA17_Chr2g0286281	52.577	194	86	2	204	396	75	263	1.69e-56	196
Msa0923130	MtrunA17_Chr1g0194541	50.256	195	90	2	204	396	277	466	8.79e-56	196
Msa0923130	MtrunA17_Chr7g0251771	46.479	213	101	3	185	396	25	225	1.01e-55	191
Msa0923130	MtrunA17_Chr5g0443691	49.744	195	91	2	204	396	85	274	3.13e-55	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0923130	AT3G58690.1	79.426	209	41	1	188	396	57	263	3.59e-114	342
Msa0923130	AT3G20530.2	47.059	221	103	3	186	396	41	257	1.33e-59	199
Msa0923130	AT1G54820.3	49.505	202	93	3	201	396	130	328	3.76e-59	198
Msa0923130	AT1G54820.4	49.505	202	93	3	201	396	130	328	3.76e-59	198
Msa0923130	AT3G20530.1	47.059	221	103	3	186	396	41	257	4.28e-59	199
Msa0923130	AT1G54820.2	49.505	202	93	3	201	396	130	328	2.16e-58	198
Msa0923130	AT1G20650.1	50.256	195	92	1	202	396	62	251	3.77e-58	197
Msa0923130	AT1G54820.1	49.505	202	93	3	201	396	130	328	5.34e-58	199
Msa0923130	AT3G07070.1	47.847	209	103	2	189	396	50	253	1.45e-57	196
Msa0923130	AT5G13160.1	52.332	193	86	2	205	396	73	260	3.86e-57	196

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Find 91 sgRNAs with CRISPR-Local

Find 419 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTCTGTGAATCACTGG+AGG	0.643898	6_2:+62921101	None:intergenic
AAGATTTGAGGGAACGATTC+TGG	0.232976	6_2:+62923459	None:intergenic
AAGCAGCTGCATTCTGCTAC+TGG	0.362535	6_2:-62921798	Msa0923130:CDS
AAGCTTGGACCTGATAGAAT+CGG	0.550115	6_2:-62921014	Msa0923130:CDS
AAGTCAAATGTAGTCGGTCC+TGG	0.519215	6_2:-62921765	Msa0923130:CDS
AATAGCACTTGAAGCTGCCA+AGG	0.561234	6_2:-62921153	Msa0923130:CDS
AATCGAAGTTGCTCTGAAAC+AGG	0.426285	6_2:-62923299	Msa0923130:intron
ACAATAACAAACTTAATAAG+CGG	0.583350	6_2:+62922770	None:intergenic
ACATAACACTATTATACAAG+CGG	0.640043	6_2:+62918340	None:intergenic
ACATGTCCACCGATTCTATC+AGG	0.359424	6_2:+62921005	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTGAGGATATGAGGATTT+AGG	+	chr6_2:62917135-62917154	None:intergenic	30.0%
TGAGAAAAGGAAAGATTTGA+GGG	+	chr6_2:62917273-62917292	None:intergenic	30.0%
TTGAGAAAAGGAAAGATTTG+AGG	+	chr6_2:62917274-62917293	None:intergenic	30.0%
GATTCATAATTCACCAGAAA+AGG	+	chr6_2:62917803-62917822	None:intergenic	30.0%
TGTTTGTAAGTTTATCACAC+TGG	-	chr6_2:62917976-62917995	Msa0923130:intron	30.0%
TGCACAATTGAGATTAATGT+TGG	-	chr6_2:62918029-62918048	Msa0923130:intron	30.0%
TTCTCAATAATCATGAGTGT+CGG	-	chr6_2:62918586-62918605	Msa0923130:intron	30.0%
GATTAATTGCATCTCATTGT+AGG	-	chr6_2:62919238-62919257	Msa0923130:intron	30.0%
GTGTATGAATTTATGGCAAA+TGG	-	chr6_2:62919341-62919360	Msa0923130:intron	30.0%
TATGAATTTATGGCAAATGG+TGG	-	chr6_2:62919344-62919363	Msa0923130:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	62917064	62923676	62917064	ID=Msa0923130;Name=Msa0923130
chr6_2	mRNA	62917064	62923676	62917064	ID=Msa0923130-mRNA-1;Parent=Msa0923130;Name=Msa0923130-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0\|0\|0\|0.3\|1\|1\|10\|0\|488
chr6_2	exon	62923474	62923676	62923474	ID=Msa0923130-mRNA-1:exon:9806;Parent=Msa0923130-mRNA-1
chr6_2	exon	62923300	62923348	62923300	ID=Msa0923130-mRNA-1:exon:9805;Parent=Msa0923130-mRNA-1
chr6_2	exon	62922760	62922831	62922760	ID=Msa0923130-mRNA-1:exon:9804;Parent=Msa0923130-mRNA-1
chr6_2	exon	62922425	62922579	62922425	ID=Msa0923130-mRNA-1:exon:9803;Parent=Msa0923130-mRNA-1
chr6_2	exon	62921659	62921965	62921659	ID=Msa0923130-mRNA-1:exon:9802;Parent=Msa0923130-mRNA-1
chr6_2	exon	62921339	62921480	62921339	ID=Msa0923130-mRNA-1:exon:9801;Parent=Msa0923130-mRNA-1
chr6_2	exon	62920963	62921221	62920963	ID=Msa0923130-mRNA-1:exon:9800;Parent=Msa0923130-mRNA-1
chr6_2	exon	62918347	62918446	62918347	ID=Msa0923130-mRNA-1:exon:9799;Parent=Msa0923130-mRNA-1
chr6_2	exon	62917267	62917357	62917267	ID=Msa0923130-mRNA-1:exon:9798;Parent=Msa0923130-mRNA-1
chr6_2	exon	62917064	62917152	62917064	ID=Msa0923130-mRNA-1:exon:9797;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62923474	62923676	62923474	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62923300	62923348	62923300	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62922760	62922831	62922760	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62922425	62922579	62922425	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62921659	62921965	62921659	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62921339	62921480	62921339	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62920963	62921221	62920963	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62918347	62918446	62918347	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62917267	62917357	62917267	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1
chr6_2	CDS	62917064	62917152	62917064	ID=Msa0923130-mRNA-1:cds;Parent=Msa0923130-mRNA-1

Gene Sequence

>Msa0923130

ATGAACTCTAGCTCCAAAAATGCAGAAATAGAACTCCAAACAGCTTCACTGAATCTCCTCAAATCACTCCTAAATCCTCATATCCTCAAATCAAACCAAAATTTCACTGAATCTTTCTCAAATCATACCAAAATCAAATCTCAAATCTTCACTGAAATCAAACCTCAGTACCAAAATCGAACCTCCTTCCTTCTTCCAGAATCTGAACTCACTCAGATTTCTGAATCCAAAATCGAAGTTGCTCTGAAACAGGTGGTTAATAAGTTGATGAGAAAGAAGCTGATTATGCATCCGCTTATTAAGTTTGTTATTGTCGCAGTGTTTGAAACAGGACAGAAGCTGGAGTGTGTGCCAGACAGCGCCCCTGCACTCTCAAATCCGGACTGCTGTCAACAACAAGCACACATGCAACAGCCCCATTTCGTAGATCAACAGATCACAGCAGCAGGACGAACCAGCATCACCAAAAGAAAATGGAGTATGAATGATAATGATTGTCACATATGGATTTGTATATTGGAATGTGATGTATATACAATATTGTTACTACAAGTTGTTACTGAGAAAAGTGATTTTTCAAACCTACAAGTTGTTACTGAGAAAGGACTTAAGGTGTTCACTTTCAAGCAGCTGCATTCTGCTACTGGAGCCTTCAGCAAGTCAAATGTAGTCGGTCCTGGTGGATTTAGACTTGTTTACCGAGGAGTGGTCAACGATGGAAGGAAGGTTGCGGTTAAATTAATGGATCGGGCAGGAAAGCAAGGAGAAGAAGAGTTTAAATTTGAGGTGGAATTGCTTAATCGGTTGCATTCTCCGTATTTGCTTGCATTGCTTGGATACTTCTCTGATCATAATCATAAGCTGCTGGTGTATGAATTTATGGCAAATGGTGGTCTGCAGGAACATCTTTATCCTGCCAGCAATAACAATTCCAGTGTCATGTCTGTTAATCTAGATTGGGAAACTCGGTTAAGAATAGCACTTGAAGCTGCCAAGGGTTTGGAATATCTTCATGAACATGTCAGTCCTCCAGTGATTCACAGAGATTTCAAGAGCAGCAACATCCTCTTGGACAAAAAATTCCATGCTAAAGTTTCTGATTTTGGATTGGCAAAGCTTGGACCTGATAGAATCGGTGGACATGTTTCAACTCGTGTTTTAGGCACTCAAGGATATGTTGCCCCCGACTGCTTTGCCAAAAAAGTGTCGTCTTATGAGTTACTATCTTGTTCTCCCATCGTTCGATCTCTGGCACTGCAAACATCTGCAACCCGCTTGTATAATAGTTTTTTTGGAGGTGTGGCTACGGATTGTTGTAAGAGGTTGCTTTGGTTTAAGCCACTTTATGAGGCAGTTTATCTCTTTGTTTTTGGGGCAGCCGAATCAGCTGCACGCTTTGTGGACGATTTTAATATTGCAAATGCAGCTGCTCCACGCTCGATTCCAGTCACAGAAAACACCAATTAG

Protein sequence

>Msa0923130

MNSSSKNAEIELQTASLNLLKSLLNPHILKSNQNFTESFSNHTKIKSQIFTEIKPQYQNRTSFLLPESELTQISESKIEVALKQVVNKLMRKKLIMHPLIKFVIVAVFETGQKLECVPDSAPALSNPDCCQQQAHMQQPHFVDQQITAAGRTSITKRKWSMNDNDCHIWICILECDVYTILLLQVVTEKSDFSNLQVVTEKGLKVFTFKQLHSATGAFSKSNVVGPGGFRLVYRGVVNDGRKVAVKLMDRAGKQGEEEFKFEVELLNRLHSPYLLALLGYFSDHNHKLLVYEFMANGGLQEHLYPASNNNSSVMSVNLDWETRLRIALEAAKGLEYLHEHVSPPVIHRDFKSSNILLDKKFHAKVSDFGLAKLGPDRIGGHVSTRVLGTQGYVAPDCFAKKVSSYELLSCSPIVRSLALQTSATRLYNSFFGGVATDCCKRLLWFKPLYEAVYLFVFGAAESAARFVDDFNIANAAAPRSIPVTENTN*