Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0928760 | KEH17201.1 | 56.164 | 73 | 27 | 2 | 71 | 143 | 20 | 87 | 5.39e-14 | 73.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0928760 | Msa0981370 | 0.810376 | 1.188769e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAATGTCAATTGAAAATT+TGG | -0.012007 | 6_2:+74818155 | Msa0928760:CDS |
| ACATTGTAGCAGAATTATTA+TGG | 0.270811 | 6_2:+74817347 | Msa0928760:CDS |
| CCAATGTCAATTGAAAATTT+GGG | 0.374516 | 6_2:+74818156 | Msa0928760:CDS |
| GAACACAAGGCTCTGATACT+AGG | 0.385177 | 6_2:+74818268 | Msa0928760:CDS |
| GGTGGTACTGGTTGGAGTGT+GGG | 0.427888 | 6_2:-74818234 | Msa0928770:CDS |
| CTAGGGCGGACTCTGGTACT+AGG | 0.435065 | 6_2:+74818286 | Msa0928760:CDS |
| CATCATCAAGCTGGTGGTAC+TGG | 0.442761 | 6_2:-74818246 | Msa0928770:CDS |
| AAAGAAATTCGCCATCCATC+AGG | 0.452746 | 6_2:-74818331 | Msa0928770:CDS |
| GCTTGTTCCATGGAAAATCA+TGG | 0.459645 | 6_2:-74817391 | None:intergenic |
| ATCTCCCATGTTCGAGCAGT+TGG | 0.472506 | 6_2:-74818376 | Msa0928770:CDS |
| TCTTTAAACGCTGCTGAAAG+AGG | 0.481700 | 6_2:+74818349 | Msa0928760:CDS |
| AACACAAGGCTCTGATACTA+GGG | 0.504137 | 6_2:+74818269 | Msa0928760:CDS |
| TGGTGGTACTGGTTGGAGTG+TGG | 0.506497 | 6_2:-74818235 | Msa0928770:CDS |
| GAAGTCCAACTGCTCGAACA+TGG | 0.522553 | 6_2:+74818371 | Msa0928760:CDS |
| GGTACTAGGACAGTACTTCA+AGG | 0.530190 | 6_2:+74818300 | Msa0928760:CDS |
| ATCAAGCTGGTGGTACTGGT+TGG | 0.538603 | 6_2:-74818242 | Msa0928770:CDS |
| TTCTTTGCAGCAAATTAAAG+CGG | 0.552212 | 6_2:-74817425 | None:intergenic |
| TCCATGGAACAAGCTTGAGA+TGG | 0.568341 | 6_2:+74817400 | Msa0928760:CDS |
| AAGTCCAACTGCTCGAACAT+GGG | 0.569234 | 6_2:+74818372 | Msa0928760:CDS |
| CCTTGTGTTCATCATCAAGC+TGG | 0.574865 | 6_2:-74818255 | Msa0928770:CDS |
| TTCATCTTCATCATCATACT+CGG | 0.589999 | 6_2:-74817319 | None:intergenic |
| TGTGTTCATCATCAAGCTGG+TGG | 0.597960 | 6_2:-74818252 | Msa0928770:CDS |
| TCTGATACTAGGGCGGACTC+TGG | 0.597979 | 6_2:+74818279 | Msa0928760:CDS |
| ACCATCTCAAGCTTGTTCCA+TGG | 0.602002 | 6_2:-74817401 | None:intergenic |
| CCAGCTTGATGATGAACACA+AGG | 0.607587 | 6_2:+74818255 | Msa0928760:CDS |
| TATTGATGATGATGTTGCTG+TGG | 0.643532 | 6_2:-74818195 | Msa0928770:CDS |
| ACAAGGCTCTGATACTAGGG+CGG | 0.690381 | 6_2:+74818272 | Msa0928760:CDS |
| ATTGCCAACATTAACATGTG+CGG | 0.698684 | 6_2:-74817457 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAATTAATATTTTTTT+TGG | - | chr6_2:74817979-74817998 | None:intergenic | 0.0% |
| !!! | TTTTAAATTAATATTTTTTT+TGG | - | chr6_2:74817979-74817998 | None:intergenic | 0.0% |
| !!! | TAAATTAATATTTTTTTTGG+CGG | - | chr6_2:74817976-74817995 | None:intergenic | 10.0% |
| !!! | TAAATTAATATTTTTTTTGG+CGG | - | chr6_2:74817976-74817995 | None:intergenic | 10.0% |
| !!! | GCAATTTATGACTTTTAAAA+TGG | + | chr6_2:74817520-74817539 | Msa0928760:intron | 20.0% |
| !! | ATATCTAAGAAAATGTCTTA+CGG | - | chr6_2:74817550-74817569 | None:intergenic | 20.0% |
| !!! | GCAATTTATGACTTTTAAAA+TGG | + | chr6_2:74817520-74817539 | Msa0928760:intron | 20.0% |
| !! | ATATCTAAGAAAATGTCTTA+CGG | - | chr6_2:74817550-74817569 | None:intergenic | 20.0% |
| ! | ACATTGTAGCAGAATTATTA+TGG | + | chr6_2:74817347-74817366 | Msa0928760:CDS | 25.0% |
| !!! | TTTAAAACCATGATTTTCCA+TGG | + | chr6_2:74817384-74817403 | Msa0928760:CDS | 25.0% |
| !!! | AAGTTTGTTACAAGAACAAA+AGG | + | chr6_2:74817636-74817655 | Msa0928760:intron | 25.0% |
| ! | TGAAGAACTCTTAGAAATTT+GGG | + | chr6_2:74817670-74817689 | Msa0928760:intron | 25.0% |
| !!! | CTTTAAAAACCGGTTTTATA+GGG | - | chr6_2:74817707-74817726 | None:intergenic | 25.0% |
| !!! | ACTTTAAAAACCGGTTTTAT+AGG | - | chr6_2:74817708-74817727 | None:intergenic | 25.0% |
| !!! | ACATTTGGTTTTCTTTTTAG+AGG | - | chr6_2:74817809-74817828 | None:intergenic | 25.0% |
| ! | CTATCATGAATTAGTGTTAT+AGG | - | chr6_2:74817848-74817867 | None:intergenic | 25.0% |
| ! | TTGAACTAATAACTATGTGT+GGG | - | chr6_2:74818054-74818073 | None:intergenic | 25.0% |
| ! | TTGAAAATGTACAAGATTGT+TGG | - | chr6_2:74818093-74818112 | None:intergenic | 25.0% |
| !! | CCAAATTTTCAATTGACATT+GGG | - | chr6_2:74818158-74818177 | None:intergenic | 25.0% |
| ! | CCAATGTCAATTGAAAATTT+GGG | + | chr6_2:74818156-74818175 | Msa0928760:CDS | 25.0% |
| ! | ACATTGTAGCAGAATTATTA+TGG | + | chr6_2:74817347-74817366 | Msa0928760:CDS | 25.0% |
| !!! | TTTAAAACCATGATTTTCCA+TGG | + | chr6_2:74817384-74817403 | Msa0928760:CDS | 25.0% |
| !!! | AAGTTTGTTACAAGAACAAA+AGG | + | chr6_2:74817636-74817655 | Msa0928760:intron | 25.0% |
| ! | TGAAGAACTCTTAGAAATTT+GGG | + | chr6_2:74817670-74817689 | Msa0928760:intron | 25.0% |
| !!! | CTTTAAAAACCGGTTTTATA+GGG | - | chr6_2:74817707-74817726 | None:intergenic | 25.0% |
| !!! | ACTTTAAAAACCGGTTTTAT+AGG | - | chr6_2:74817708-74817727 | None:intergenic | 25.0% |
| !!! | ACATTTGGTTTTCTTTTTAG+AGG | - | chr6_2:74817809-74817828 | None:intergenic | 25.0% |
| ! | CTATCATGAATTAGTGTTAT+AGG | - | chr6_2:74817848-74817867 | None:intergenic | 25.0% |
| ! | TTGAACTAATAACTATGTGT+GGG | - | chr6_2:74818054-74818073 | None:intergenic | 25.0% |
| ! | TTGAAAATGTACAAGATTGT+TGG | - | chr6_2:74818093-74818112 | None:intergenic | 25.0% |
| !! | CCAAATTTTCAATTGACATT+GGG | - | chr6_2:74818158-74818177 | None:intergenic | 25.0% |
| ! | CCAATGTCAATTGAAAATTT+GGG | + | chr6_2:74818156-74818175 | Msa0928760:CDS | 25.0% |
| TTCATCTTCATCATCATACT+CGG | - | chr6_2:74817322-74817341 | None:intergenic | 30.0% | |
| TTCTTTGCAGCAAATTAAAG+CGG | - | chr6_2:74817428-74817447 | None:intergenic | 30.0% | |
| GTGAAGAACTCTTAGAAATT+TGG | + | chr6_2:74817669-74817688 | Msa0928760:intron | 30.0% | |
| GAAGAACTCTTAGAAATTTG+GGG | + | chr6_2:74817671-74817690 | Msa0928760:intron | 30.0% | |
| CTAAATCATCCCTATAAAAC+CGG | + | chr6_2:74817695-74817714 | Msa0928760:intron | 30.0% | |
| GAGAGAAAGTTGTCAAAATA+TGG | + | chr6_2:74817732-74817751 | Msa0928760:intron | 30.0% | |
| !! | TGGTTTTCTTTTTAGAGGTT+TGG | - | chr6_2:74817804-74817823 | None:intergenic | 30.0% |
| TCATGAATTAGTGTTATAGG+TGG | - | chr6_2:74817845-74817864 | None:intergenic | 30.0% | |
| CTTGAACTAATAACTATGTG+TGG | - | chr6_2:74818055-74818074 | None:intergenic | 30.0% | |
| CCCAATGTCAATTGAAAATT+TGG | + | chr6_2:74818155-74818174 | Msa0928760:CDS | 30.0% | |
| ! | CCCAAATTTTCAATTGACAT+TGG | - | chr6_2:74818159-74818178 | None:intergenic | 30.0% |
| ! | TTCAAGGTATATTTTCCTGA+TGG | + | chr6_2:74818316-74818335 | Msa0928760:CDS | 30.0% |
| TTCATCTTCATCATCATACT+CGG | - | chr6_2:74817322-74817341 | None:intergenic | 30.0% | |
| TTCTTTGCAGCAAATTAAAG+CGG | - | chr6_2:74817428-74817447 | None:intergenic | 30.0% | |
| GTGAAGAACTCTTAGAAATT+TGG | + | chr6_2:74817669-74817688 | Msa0928760:intron | 30.0% | |
| GAAGAACTCTTAGAAATTTG+GGG | + | chr6_2:74817671-74817690 | Msa0928760:intron | 30.0% | |
| CTAAATCATCCCTATAAAAC+CGG | + | chr6_2:74817695-74817714 | Msa0928760:intron | 30.0% | |
| GAGAGAAAGTTGTCAAAATA+TGG | + | chr6_2:74817732-74817751 | Msa0928760:intron | 30.0% | |
| !! | TGGTTTTCTTTTTAGAGGTT+TGG | - | chr6_2:74817804-74817823 | None:intergenic | 30.0% |
| TCATGAATTAGTGTTATAGG+TGG | - | chr6_2:74817845-74817864 | None:intergenic | 30.0% | |
| CTTGAACTAATAACTATGTG+TGG | - | chr6_2:74818055-74818074 | None:intergenic | 30.0% | |
| CCCAATGTCAATTGAAAATT+TGG | + | chr6_2:74818155-74818174 | Msa0928760:CDS | 30.0% | |
| ! | CCCAAATTTTCAATTGACAT+TGG | - | chr6_2:74818159-74818178 | None:intergenic | 30.0% |
| ! | TTCAAGGTATATTTTCCTGA+TGG | + | chr6_2:74818316-74818335 | Msa0928760:CDS | 30.0% |
| ATTGCCAACATTAACATGTG+CGG | - | chr6_2:74817460-74817479 | None:intergenic | 35.0% | |
| TAATGTTGGCAATTGCTTGT+TGG | + | chr6_2:74817467-74817486 | Msa0928760:intron | 35.0% | |
| AATGTTGGCAATTGCTTGTT+GGG | + | chr6_2:74817468-74817487 | Msa0928760:intron | 35.0% | |
| ! | CTCTCTCTCACTTTAAAAAC+CGG | - | chr6_2:74817717-74817736 | None:intergenic | 35.0% |
| TGTGGGACTTCTATAATTGA+TGG | - | chr6_2:74818037-74818056 | None:intergenic | 35.0% | |
| !! | TATTGATGATGATGTTGCTG+TGG | - | chr6_2:74818198-74818217 | Msa0928770:CDS | 35.0% |
| ! | AGGTATATTTTCCTGATGGA+TGG | + | chr6_2:74818320-74818339 | Msa0928760:CDS | 35.0% |
| ATTGCCAACATTAACATGTG+CGG | - | chr6_2:74817460-74817479 | None:intergenic | 35.0% | |
| TAATGTTGGCAATTGCTTGT+TGG | + | chr6_2:74817467-74817486 | Msa0928760:intron | 35.0% | |
| AATGTTGGCAATTGCTTGTT+GGG | + | chr6_2:74817468-74817487 | Msa0928760:intron | 35.0% | |
| ! | CTCTCTCTCACTTTAAAAAC+CGG | - | chr6_2:74817717-74817736 | None:intergenic | 35.0% |
| TGTGGGACTTCTATAATTGA+TGG | - | chr6_2:74818037-74818056 | None:intergenic | 35.0% | |
| !! | TATTGATGATGATGTTGCTG+TGG | - | chr6_2:74818198-74818217 | Msa0928770:CDS | 35.0% |
| ! | AGGTATATTTTCCTGATGGA+TGG | + | chr6_2:74818320-74818339 | Msa0928760:CDS | 35.0% |
| GCTTGTTCCATGGAAAATCA+TGG | - | chr6_2:74817394-74817413 | None:intergenic | 40.0% | |
| ! | CTCTTTTGCACATGAACTTC+AGG | - | chr6_2:74817877-74817896 | None:intergenic | 40.0% |
| ! | AACACAAGGCTCTGATACTA+GGG | + | chr6_2:74818269-74818288 | Msa0928760:CDS | 40.0% |
| AAAGAAATTCGCCATCCATC+AGG | - | chr6_2:74818334-74818353 | Msa0928770:CDS | 40.0% | |
| TCTTTAAACGCTGCTGAAAG+AGG | + | chr6_2:74818349-74818368 | Msa0928760:CDS | 40.0% | |
| GCTTGTTCCATGGAAAATCA+TGG | - | chr6_2:74817394-74817413 | None:intergenic | 40.0% | |
| ! | CTCTTTTGCACATGAACTTC+AGG | - | chr6_2:74817877-74817896 | None:intergenic | 40.0% |
| ! | AACACAAGGCTCTGATACTA+GGG | + | chr6_2:74818269-74818288 | Msa0928760:CDS | 40.0% |
| AAAGAAATTCGCCATCCATC+AGG | - | chr6_2:74818334-74818353 | Msa0928770:CDS | 40.0% | |
| TCTTTAAACGCTGCTGAAAG+AGG | + | chr6_2:74818349-74818368 | Msa0928760:CDS | 40.0% | |
| TCCATGGAACAAGCTTGAGA+TGG | + | chr6_2:74817400-74817419 | Msa0928760:CDS | 45.0% | |
| ! | ACCATCTCAAGCTTGTTCCA+TGG | - | chr6_2:74817404-74817423 | None:intergenic | 45.0% |
| AGCACCGCACATGTTAATGT+TGG | + | chr6_2:74817453-74817472 | Msa0928760:intron | 45.0% | |
| ! | GGTCGACTTTGGATCACATT+TGG | - | chr6_2:74817824-74817843 | None:intergenic | 45.0% |
| ! | GTGTTATAGGTGGTCGACTT+TGG | - | chr6_2:74817835-74817854 | None:intergenic | 45.0% |
| TGTGTTCATCATCAAGCTGG+TGG | - | chr6_2:74818255-74818274 | Msa0928770:CDS | 45.0% | |
| CCTTGTGTTCATCATCAAGC+TGG | - | chr6_2:74818258-74818277 | Msa0928770:CDS | 45.0% | |
| !! | CCAGCTTGATGATGAACACA+AGG | + | chr6_2:74818255-74818274 | Msa0928760:CDS | 45.0% |
| ! | GAACACAAGGCTCTGATACT+AGG | + | chr6_2:74818268-74818287 | Msa0928760:CDS | 45.0% |
| GGTACTAGGACAGTACTTCA+AGG | + | chr6_2:74818300-74818319 | Msa0928760:CDS | 45.0% | |
| AAGTCCAACTGCTCGAACAT+GGG | + | chr6_2:74818372-74818391 | Msa0928760:CDS | 45.0% | |
| TCCATGGAACAAGCTTGAGA+TGG | + | chr6_2:74817400-74817419 | Msa0928760:CDS | 45.0% | |
| ! | ACCATCTCAAGCTTGTTCCA+TGG | - | chr6_2:74817404-74817423 | None:intergenic | 45.0% |
| AGCACCGCACATGTTAATGT+TGG | + | chr6_2:74817453-74817472 | Msa0928760:intron | 45.0% | |
| ! | GGTCGACTTTGGATCACATT+TGG | - | chr6_2:74817824-74817843 | None:intergenic | 45.0% |
| ! | GTGTTATAGGTGGTCGACTT+TGG | - | chr6_2:74817835-74817854 | None:intergenic | 45.0% |
| TGTGTTCATCATCAAGCTGG+TGG | - | chr6_2:74818255-74818274 | Msa0928770:CDS | 45.0% | |
| CCTTGTGTTCATCATCAAGC+TGG | - | chr6_2:74818258-74818277 | Msa0928770:CDS | 45.0% | |
| !! | CCAGCTTGATGATGAACACA+AGG | + | chr6_2:74818255-74818274 | Msa0928760:CDS | 45.0% |
| ! | GAACACAAGGCTCTGATACT+AGG | + | chr6_2:74818268-74818287 | Msa0928760:CDS | 45.0% |
| GGTACTAGGACAGTACTTCA+AGG | + | chr6_2:74818300-74818319 | Msa0928760:CDS | 45.0% | |
| AAGTCCAACTGCTCGAACAT+GGG | + | chr6_2:74818372-74818391 | Msa0928760:CDS | 45.0% | |
| !! | ATCAAGCTGGTGGTACTGGT+TGG | - | chr6_2:74818245-74818264 | Msa0928770:CDS | 50.0% |
| CATCATCAAGCTGGTGGTAC+TGG | - | chr6_2:74818249-74818268 | Msa0928770:CDS | 50.0% | |
| ! | ACAAGGCTCTGATACTAGGG+CGG | + | chr6_2:74818272-74818291 | Msa0928760:CDS | 50.0% |
| GAAGTCCAACTGCTCGAACA+TGG | + | chr6_2:74818371-74818390 | Msa0928760:CDS | 50.0% | |
| ATCTCCCATGTTCGAGCAGT+TGG | - | chr6_2:74818379-74818398 | Msa0928770:CDS | 50.0% | |
| !! | ATCAAGCTGGTGGTACTGGT+TGG | - | chr6_2:74818245-74818264 | Msa0928770:CDS | 50.0% |
| CATCATCAAGCTGGTGGTAC+TGG | - | chr6_2:74818249-74818268 | Msa0928770:CDS | 50.0% | |
| ! | ACAAGGCTCTGATACTAGGG+CGG | + | chr6_2:74818272-74818291 | Msa0928760:CDS | 50.0% |
| GAAGTCCAACTGCTCGAACA+TGG | + | chr6_2:74818371-74818390 | Msa0928760:CDS | 50.0% | |
| ATCTCCCATGTTCGAGCAGT+TGG | - | chr6_2:74818379-74818398 | Msa0928770:CDS | 50.0% | |
| !! | GGTGGTACTGGTTGGAGTGT+GGG | - | chr6_2:74818237-74818256 | Msa0928770:CDS | 55.0% |
| !! | TGGTGGTACTGGTTGGAGTG+TGG | - | chr6_2:74818238-74818257 | Msa0928770:CDS | 55.0% |
| ! | TCTGATACTAGGGCGGACTC+TGG | + | chr6_2:74818279-74818298 | Msa0928760:CDS | 55.0% |
| !! | GGTGGTACTGGTTGGAGTGT+GGG | - | chr6_2:74818237-74818256 | Msa0928770:CDS | 55.0% |
| !! | TGGTGGTACTGGTTGGAGTG+TGG | - | chr6_2:74818238-74818257 | Msa0928770:CDS | 55.0% |
| ! | TCTGATACTAGGGCGGACTC+TGG | + | chr6_2:74818279-74818298 | Msa0928760:CDS | 55.0% |
| ! | CTAGGGCGGACTCTGGTACT+AGG | + | chr6_2:74818286-74818305 | Msa0928760:CDS | 60.0% |
| ! | CTAGGGCGGACTCTGGTACT+AGG | + | chr6_2:74818286-74818305 | Msa0928760:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 74817300 | 74818417 | 74817300 | ID=Msa0928760;Name=Msa0928760 |
| chr6_2 | mRNA | 74817300 | 74818417 | 74817300 | ID=Msa0928760-mRNA-1;Parent=Msa0928760;Name=Msa0928760-mRNA-1;_AED=0.36;_eAED=0.40;_QI=0|0|0|0.5|1|1|2|0|153 |
| chr6_2 | exon | 74817300 | 74817464 | 74817300 | ID=Msa0928760-mRNA-1:exon:12719;Parent=Msa0928760-mRNA-1 |
| chr6_2 | exon | 74818121 | 74818417 | 74818121 | ID=Msa0928760-mRNA-1:exon:12720;Parent=Msa0928760-mRNA-1 |
| chr6_2 | CDS | 74817300 | 74817464 | 74817300 | ID=Msa0928760-mRNA-1:cds;Parent=Msa0928760-mRNA-1 |
| chr6_2 | CDS | 74818121 | 74818417 | 74818121 | ID=Msa0928760-mRNA-1:cds;Parent=Msa0928760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
