AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0933700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGAAGTTTACCAAATTT+AGG 0.154830 6_3:-2308419 Msa0933700:three_prime_UTR
GCAAATGTTCACAAAATATT+TGG 0.192290 6_3:-2308904 Msa0933700:three_prime_UTR
AAGAAGCGTGAATGGGTTTC+TGG 0.220020 6_3:-2311753 Msa0933700:CDS
ACAATAGGAAGCCCTAAATT+TGG 0.227301 6_3:+2308407 None:intergenic
TAAACTTCAATTTATTTAAT+AGG 0.229927 6_3:+2308430 None:intergenic
ATTGAAGTTTACCAAATTTA+GGG 0.238132 6_3:-2308418 Msa0933700:three_prime_UTR
TTGTTGGAGCTAGTTTAATT+TGG 0.276803 6_3:+2308476 None:intergenic
GCAAACTTATGAGGCTCTTC+TGG 0.277156 6_3:+2308925 None:intergenic
AACAATTCAATCTTCTTTGA+TGG 0.280338 6_3:-2311828 Msa0933700:CDS
GGCTCTTCTGGTGGGAAATA+AGG 0.304156 6_3:+2308937 None:intergenic
GGTTCTTCTTCTCTTGGTCT+TGG 0.305065 6_3:-2308673 Msa0933700:three_prime_UTR
CATCAAGGTTCTTCTTCTCT+TGG 0.331910 6_3:-2308679 Msa0933700:three_prime_UTR
GGATATTTAAGCTTAGATTA+TGG 0.336382 6_3:-2309037 Msa0933700:intron
AATATTCAATACTTTACAAT+AGG 0.346079 6_3:+2308392 None:intergenic
TTATATATCACCTTTGTTGT+AGG 0.350339 6_3:+2308585 None:intergenic
ATGGAATGTACCTTCCAAAA+TGG 0.361658 6_3:-2309792 Msa0933700:CDS
TGAACATTTGCAAACTTATG+AGG 0.383834 6_3:+2308916 None:intergenic
GAATCATTGCTCAAATTAGA+TGG 0.418926 6_3:+2308619 None:intergenic
ATCTACAACCTTCTTAATCA+TGG 0.419380 6_3:+2311787 None:intergenic
AAAAGAGGAAGAAGCGTGAA+TGG 0.424416 6_3:-2311761 Msa0933700:CDS
CAATCTTCTTTGATGGTGCA+TGG 0.426992 6_3:-2311821 Msa0933700:CDS
TATTGAGGCATATGTCTTGT+TGG 0.441039 6_3:+2308460 None:intergenic
ATGATTTAAGCGAATCAAAT+CGG 0.450299 6_3:+2308699 None:intergenic
GTAGTGAATATGGACGTTGC+AGG 0.453956 6_3:-2308506 Msa0933700:three_prime_UTR
ATTTGAGCAATGATTCTTGT+GGG 0.463152 6_3:-2308612 Msa0933700:three_prime_UTR
GGTATGCAATTCGGGGTGAA+TGG 0.471187 6_3:-2309811 Msa0933700:CDS
GATTGATTCCAACGCTTCAC+AGG 0.486227 6_3:-2311998 Msa0933700:intron
TTGATTCGCTTAAATCATCA+AGG 0.486236 6_3:-2308694 Msa0933700:three_prime_UTR
AATCTTCTTTGATGGTGCAT+GGG 0.491436 6_3:-2311820 Msa0933700:CDS
TTAATAGGAGACTAATATTG+AGG 0.497879 6_3:+2308445 None:intergenic
AAAGAGGAAGAAGCGTGAAT+GGG 0.500677 6_3:-2311760 Msa0933700:CDS
ACAACTCATTAAGCTTCAAA+AGG 0.501141 6_3:-2308740 Msa0933700:three_prime_UTR
ATTTGCAACCATGATTAAGA+AGG 0.504021 6_3:-2311795 Msa0933700:CDS
AGAAGCGTGAATGGGTTTCT+GGG 0.507262 6_3:-2311752 Msa0933700:intron
TGTGGGGATTCCTACAACAA+AGG 0.509877 6_3:-2308595 Msa0933700:three_prime_UTR
ATCTTCTTTGATGGTGCATG+GGG 0.520548 6_3:-2311819 Msa0933700:CDS
AGGATTAAAGATCCAGTGTA+TGG 0.525146 6_3:-2308793 Msa0933700:three_prime_UTR
AACTTATGAGGCTCTTCTGG+TGG 0.540828 6_3:+2308928 None:intergenic
AATTTGAGCAATGATTCTTG+TGG 0.553273 6_3:-2308613 Msa0933700:three_prime_UTR
AAGGTTGTAGATGAGAAAAG+AGG 0.556261 6_3:-2311776 Msa0933700:CDS
ACTGTATCTTCTCTTTGATG+AGG 0.567592 6_3:+2308844 None:intergenic
GATCCAGTGTATGGTTGTGT+TGG 0.578362 6_3:-2308784 Msa0933700:three_prime_UTR
TTTGTGTACCTGTGAAGCGT+TGG 0.586701 6_3:+2311990 None:intergenic
AGAAACTTGCAAGCAGCACA+TGG 0.591215 6_3:+2308994 None:intergenic
TAATGTTTCAAAGAGTCTAG+AGG 0.606434 6_3:-2308552 Msa0933700:three_prime_UTR
ACTTATGAGGCTCTTCTGGT+GGG 0.610191 6_3:+2308929 None:intergenic
TTTGAGCAATGATTCTTGTG+GGG 0.613138 6_3:-2308611 Msa0933700:three_prime_UTR
AGAAACTTCCACATGTCTAG+TGG 0.624168 6_3:-2309753 Msa0933700:intron
TAGCTTATGAAGCTGAAGCA+AGG 0.625096 6_3:-2308813 Msa0933700:three_prime_UTR
TGGAGAATTTGAGTAGCTTG+AGG 0.626001 6_3:+2309014 None:intergenic
GCTCCAACACAACCATACAC+TGG 0.629286 6_3:+2308781 None:intergenic
CACACCTACCACTAGACATG+TGG 0.632123 6_3:+2309745 None:intergenic
TTTGGAAGGTATGCAATTCG+GGG 0.646604 6_3:-2309818 Msa0933700:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATACGAAAATTTAAAAT+TGG - chr6_3:2308434-2308453 Msa0933700:three_prime_UTR 10.0%
!! ATCAAAATATTTATAACTTT+CGG - chr6_3:2308972-2308991 Msa0933700:three_prime_UTR 10.0%
!!! GCATTAAAAATTTAAAATTA+TGG + chr6_3:2309200-2309219 None:intergenic 10.0%
!!! ATGCAATAATATTTTTTTAA+TGG + chr6_3:2309668-2309687 None:intergenic 10.0%
!! ACTTAAATATATATAATTCT+TGG + chr6_3:2310823-2310842 None:intergenic 10.0%
!!! TTTCAATTATAATTTTGTTA+AGG - chr6_3:2310906-2310925 Msa0933700:intron 10.0%
!! AATCTAAATGAAAAAATTAA+TGG + chr6_3:2311168-2311187 None:intergenic 10.0%
!! TAAACTTCAATTTATTTAAT+AGG + chr6_3:2311937-2311956 None:intergenic 10.0%
!!! TAAAGTGTTTTTTAATCATT+TGG - chr6_3:2308680-2308699 Msa0933700:three_prime_UTR 15.0%
!!! TTAATAGTATTTTAATTGGA+TGG + chr6_3:2308775-2308794 None:intergenic 15.0%
!!! GGATTTAATAGTATTTTAAT+TGG + chr6_3:2308779-2308798 None:intergenic 15.0%
!! TTAAAATACTATTAAATCCA+TGG - chr6_3:2308780-2308799 Msa0933700:three_prime_UTR 15.0%
!!! TTCATTTATTTTTTGACATT+AGG - chr6_3:2309299-2309318 Msa0933700:intron 15.0%
!!! TTTTTGTTACATAAGATTTA+GGG + chr6_3:2309869-2309888 None:intergenic 15.0%
!!! TTTTTTGTTACATAAGATTT+AGG + chr6_3:2309870-2309889 None:intergenic 15.0%
!!! AAGGTACTTAAATTTTTTTT+GGG + chr6_3:2309970-2309989 None:intergenic 15.0%
!!! AAAGGTACTTAAATTTTTTT+TGG + chr6_3:2309971-2309990 None:intergenic 15.0%
!! AATAAATTACAAGTTAAACT+TGG - chr6_3:2310087-2310106 Msa0933700:intron 15.0%
!! AATCACAAAAAAAAATTTGA+GGG + chr6_3:2310885-2310904 None:intergenic 15.0%
!! AAATCACAAAAAAAAATTTG+AGG + chr6_3:2310886-2310905 None:intergenic 15.0%
!! CATAATATCAAATAAAAACA+GGG + chr6_3:2311224-2311243 None:intergenic 15.0%
!! TCATAATATCAAATAAAAAC+AGG + chr6_3:2311225-2311244 None:intergenic 15.0%
!!! TTGTCAATTTAAATAGTTTT+TGG - chr6_3:2311257-2311276 Msa0933700:intron 15.0%
!!! AATTTAAATAGTTTTTGGTT+TGG - chr6_3:2311262-2311281 Msa0933700:intron 15.0%
!!! ATAATTGTGTTTTAATTTGT+TGG - chr6_3:2311296-2311315 Msa0933700:intron 15.0%
!!! TAATTGTGTTTTAATTTGTT+GGG - chr6_3:2311297-2311316 Msa0933700:intron 15.0%
!!! TATTTTAAATGTAGTGAATA+TGG - chr6_3:2311848-2311867 Msa0933700:CDS 15.0%
!! AATATTCAATACTTTACAAT+AGG + chr6_3:2311975-2311994 None:intergenic 15.0%
!!! AATATTTTGTGTAATATACA+AGG - chr6_3:2311989-2312008 Msa0933700:intron 15.0%
!!! TTCGTATTATTATCAAGTTT+TGG + chr6_3:2308424-2308443 None:intergenic 20.0%
!! ATGATGCTAATTATATTTAG+TGG + chr6_3:2309162-2309181 None:intergenic 20.0%
!! TATCTAAATTAAACTCATTG+AGG - chr6_3:2309260-2309279 Msa0933700:intron 20.0%
!! ATCTAAATTAAACTCATTGA+GGG - chr6_3:2309261-2309280 Msa0933700:intron 20.0%
!! ATAGTTAAAGAATAAGAGTT+TGG - chr6_3:2309339-2309358 Msa0933700:intron 20.0%
!!! TATTTATTAACTTGAGTACA+AGG - chr6_3:2309467-2309486 Msa0933700:intron 20.0%
!!! ATTTATTAACTTGAGTACAA+GGG - chr6_3:2309468-2309487 Msa0933700:intron 20.0%
!!! ATTTGATCTGAATTTTGTAT+TGG + chr6_3:2309504-2309523 None:intergenic 20.0%
!!! AGAATATTGTTTTCTCTAAT+TGG + chr6_3:2309557-2309576 None:intergenic 20.0%
!!! TAATTTTCTACTCTTTAATG+AGG + chr6_3:2309767-2309786 None:intergenic 20.0%
!! TTAGTATATAAACTAGCAAT+TGG - chr6_3:2310030-2310049 Msa0933700:intron 20.0%
!! TAGTATATAAACTAGCAATT+GGG - chr6_3:2310031-2310050 Msa0933700:intron 20.0%
!!! TGGTTTTTGATTTCTTTTTT+TGG - chr6_3:2310528-2310547 Msa0933700:intron 20.0%
!!! TTTTGATTTCTTTTTTTGGA+AGG - chr6_3:2310532-2310551 Msa0933700:intron 20.0%
!! TATATCTCTAAACTAAACTA+GGG + chr6_3:2310743-2310762 None:intergenic 20.0%
!! ATATATCTCTAAACTAAACT+AGG + chr6_3:2310744-2310763 None:intergenic 20.0%
!!! CTTTTTTTTTTTGAAACAGA+GGG + chr6_3:2310852-2310871 None:intergenic 20.0%
!!! ACTTTTTTTTTTTGAAACAG+AGG + chr6_3:2310853-2310872 None:intergenic 20.0%
!!! TTGTTAAGGATTTTTTTTAG+AGG - chr6_3:2310920-2310939 Msa0933700:intron 20.0%
!!! TGTTAAGGATTTTTTTTAGA+GGG - chr6_3:2310921-2310940 Msa0933700:intron 20.0%
!! AGGGAAAAATATCAAAATAA+TGG + chr6_3:2311205-2311224 None:intergenic 20.0%
!! AATTGAAGTTTACCAAATTT+AGG - chr6_3:2311945-2311964 Msa0933700:intron 20.0%
!! ATTGAAGTTTACCAAATTTA+GGG - chr6_3:2311946-2311965 Msa0933700:intron 20.0%
! AGAGTATGAATCAAATTGAA+TGG - chr6_3:2308457-2308476 Msa0933700:three_prime_UTR 25.0%
! ATCAAATTGAATGGTGATAA+AGG - chr6_3:2308466-2308485 Msa0933700:three_prime_UTR 25.0%
! AACAATTCAATCTTCTTTGA+TGG - chr6_3:2308536-2308555 Msa0933700:three_prime_UTR 25.0%
!!! AGTGTTTTTTAATCATTTGG+TGG - chr6_3:2308683-2308702 Msa0933700:three_prime_UTR 25.0%
!!! GTGTTTTTTAATCATTTGGT+GGG - chr6_3:2308684-2308703 Msa0933700:three_prime_UTR 25.0%
!!! TGTTTTTTAATCATTTGGTG+GGG - chr6_3:2308685-2308704 Msa0933700:three_prime_UTR 25.0%
! TGATTTCTTATACCCAATAA+AGG + chr6_3:2308956-2308975 None:intergenic 25.0%
! TGACGTTAACTATTTAATTG+TGG + chr6_3:2309092-2309111 None:intergenic 25.0%
! TGCTAATTATATTTAGTGGA+TGG + chr6_3:2309158-2309177 None:intergenic 25.0%
!! ATATTGTTTTCTCTAATTGG+TGG + chr6_3:2309554-2309573 None:intergenic 25.0%
! ATAAGATTAAGCGAATTTCA+TGG + chr6_3:2309616-2309635 None:intergenic 25.0%
! TTATCTAATTTGGCTAACTA+CGG + chr6_3:2309712-2309731 None:intergenic 25.0%
! TTGTAAAGGAAAAAAATGGT+AGG + chr6_3:2309743-2309762 None:intergenic 25.0%
! AGGTTTGTAAAGGAAAAAAA+TGG + chr6_3:2309747-2309766 None:intergenic 25.0%
!!! AAAATTTTAAGTGGATGGAT+TGG + chr6_3:2309823-2309842 None:intergenic 25.0%
! TTGTAGTCTATTTCAACTTT+AGG + chr6_3:2309920-2309939 None:intergenic 25.0%
!!! TAAGTACCTTTTAAGATAAC+CGG - chr6_3:2309980-2309999 Msa0933700:intron 25.0%
! AGTATATAAACTAGCAATTG+GGG - chr6_3:2310032-2310051 Msa0933700:intron 25.0%
! AAAAGACAATGTGAAAAAGA+AGG + chr6_3:2310169-2310188 None:intergenic 25.0%
! AGGTAGTTATAAATAGTTGA+AGG + chr6_3:2310288-2310307 None:intergenic 25.0%
! TCAACTATTTATAACTACCT+AGG - chr6_3:2310288-2310307 Msa0933700:intron 25.0%
! CAACTATTTATAACTACCTA+GGG - chr6_3:2310289-2310308 Msa0933700:intron 25.0%
! TTTATGAATATTAGAACCCT+AGG + chr6_3:2310308-2310327 None:intergenic 25.0%
!! TTTGAGATGAAAATTATACC+TGG + chr6_3:2310447-2310466 None:intergenic 25.0%
! AACTTCAACTAAAAACCAAA+GGG + chr6_3:2310485-2310504 None:intergenic 25.0%
! AAACTTCAACTAAAAACCAA+AGG + chr6_3:2310486-2310505 None:intergenic 25.0%
!! TTTAGTTGAAGTTTTGCTTT+TGG - chr6_3:2310492-2310511 Msa0933700:intron 25.0%
!! ATTTTCAACTCTTTCCATTT+TGG + chr6_3:2310589-2310608 None:intergenic 25.0%
!!! TTTTAGAGGGAATTTTGTTA+AGG - chr6_3:2310934-2310953 Msa0933700:intron 25.0%
!!! AACCAATTGTTTTTTGTGTA+GGG + chr6_3:2311110-2311129 None:intergenic 25.0%
!!! AAACCAATTGTTTTTTGTGT+AGG + chr6_3:2311111-2311130 None:intergenic 25.0%
!! AAACAATTGGTTTCAATTGT+TGG - chr6_3:2311118-2311137 Msa0933700:intron 25.0%
!! AACAATTGGTTTCAATTGTT+GGG - chr6_3:2311119-2311138 Msa0933700:intron 25.0%
! GGATATTTAAGCTTAGATTA+TGG - chr6_3:2311327-2311346 Msa0933700:intron 25.0%
! GCAAATGTTCACAAAATATT+TGG - chr6_3:2311460-2311479 Msa0933700:intron 25.0%
! ATGATTTAAGCGAATCAAAT+CGG + chr6_3:2311668-2311687 None:intergenic 25.0%
!! TTATATATCACCTTTGTTGT+AGG + chr6_3:2311782-2311801 None:intergenic 25.0%
! TTAATAGGAGACTAATATTG+AGG + chr6_3:2311922-2311941 None:intergenic 25.0%
ATTGAATGGTGATAAAGGTT+TGG - chr6_3:2308471-2308490 Msa0933700:three_prime_UTR 30.0%
ATTTGCAACCATGATTAAGA+AGG - chr6_3:2308569-2308588 Msa0933700:three_prime_UTR 30.0%
ATCTACAACCTTCTTAATCA+TGG + chr6_3:2308580-2308599 None:intergenic 30.0%
! TAATTGTTGCAACTTTTTGG+TGG + chr6_3:2308734-2308753 None:intergenic 30.0%
! TGGTAATTGTTGCAACTTTT+TGG + chr6_3:2308737-2308756 None:intergenic 30.0%
TATTAAATCCATGGCTGTAA+AGG - chr6_3:2308789-2308808 Msa0933700:three_prime_UTR 30.0%
ATTAAATCCATGGCTGTAAA+GGG - chr6_3:2308790-2308809 Msa0933700:three_prime_UTR 30.0%
!!! AAACTCTTTTTTCCCATGAT+GGG + chr6_3:2308822-2308841 None:intergenic 30.0%
TTTCTTATACCCAATAAAGG+TGG + chr6_3:2308953-2308972 None:intergenic 30.0%
TGATAAGGATGTTTGTACAA+GGG + chr6_3:2309035-2309054 None:intergenic 30.0%
TTGATAAGGATGTTTGTACA+AGG + chr6_3:2309036-2309055 None:intergenic 30.0%
CAAACATCCTTATCAAACTT+TGG - chr6_3:2309040-2309059 Msa0933700:intron 30.0%
TTATGTGCCAAAGTTTGATA+AGG + chr6_3:2309050-2309069 None:intergenic 30.0%
! AGAATAAGAGTTTGGTTTAG+TGG - chr6_3:2309347-2309366 Msa0933700:intron 30.0%
AGAGATTTGCATTTCTTGAA+GGG + chr6_3:2309424-2309443 None:intergenic 30.0%
AAGAGATTTGCATTTCTTGA+AGG + chr6_3:2309425-2309444 None:intergenic 30.0%
TCTTGGTTCATTAATAGAGA+AGG + chr6_3:2309593-2309612 None:intergenic 30.0%
TGGAAAAGTAGTGAATATTG+CGG + chr6_3:2309648-2309667 None:intergenic 30.0%
AAAGGAAAAAAATGGTAGGA+AGG + chr6_3:2309739-2309758 None:intergenic 30.0%
CTCTTTAATGAGGTTTGTAA+AGG + chr6_3:2309757-2309776 None:intergenic 30.0%
! TTTGGGAGCAAAATTTTAAG+TGG + chr6_3:2309832-2309851 None:intergenic 30.0%
! GGGAGAGAGTATAAAAATTT+GGG + chr6_3:2309849-2309868 None:intergenic 30.0%
! AGGGAGAGAGTATAAAAATT+TGG + chr6_3:2309850-2309869 None:intergenic 30.0%
!!! TAGGTCGTATTTTTTAGTTG+TGG + chr6_3:2309901-2309920 None:intergenic 30.0%
!! TTTTTGGAAGGTATGCAATT+CGG - chr6_3:2310544-2310563 Msa0933700:intron 30.0%
! TCTCCTTTTCTTTCTTTTTG+TGG + chr6_3:2310683-2310702 None:intergenic 30.0%
TCTAAACTAAACTAGGGTTA+TGG + chr6_3:2310737-2310756 None:intergenic 30.0%
!!! ACCAATTGTTTTTTGTGTAG+GGG + chr6_3:2311109-2311128 None:intergenic 30.0%
!! TTTAATTTGTTGGGTTGTGA+TGG - chr6_3:2311306-2311325 Msa0933700:intron 30.0%
TGAACATTTGCAAACTTATG+AGG + chr6_3:2311451-2311470 None:intergenic 30.0%
ACAACTCATTAAGCTTCAAA+AGG - chr6_3:2311624-2311643 Msa0933700:intron 30.0%
! TTGATTCGCTTAAATCATCA+AGG - chr6_3:2311670-2311689 Msa0933700:intron 30.0%
GAATCATTGCTCAAATTAGA+TGG + chr6_3:2311748-2311767 None:intergenic 30.0%
AATTTGAGCAATGATTCTTG+TGG - chr6_3:2311751-2311770 Msa0933700:intron 30.0%
ATTTGAGCAATGATTCTTGT+GGG - chr6_3:2311752-2311771 Msa0933700:intron 30.0%
TAATGTTTCAAAGAGTCTAG+AGG - chr6_3:2311812-2311831 Msa0933700:CDS 30.0%
! TTGTTGGAGCTAGTTTAATT+TGG + chr6_3:2311891-2311910 None:intergenic 30.0%
GATAAAGGTTTGGATTTGAG+TGG - chr6_3:2308481-2308500 Msa0933700:three_prime_UTR 35.0%
! GTTTGGATTTGAGTGGATTT+AGG - chr6_3:2308488-2308507 Msa0933700:three_prime_UTR 35.0%
!! AATCTTCTTTGATGGTGCAT+GGG - chr6_3:2308544-2308563 Msa0933700:three_prime_UTR 35.0%
AAGGTTGTAGATGAGAAAAG+AGG - chr6_3:2308588-2308607 Msa0933700:three_prime_UTR 35.0%
!! TTTTAATCATTTGGTGGGGT+TGG - chr6_3:2308689-2308708 Msa0933700:three_prime_UTR 35.0%
GATGGTTGAGATCTTTAACT+TGG + chr6_3:2308757-2308776 None:intergenic 35.0%
!!! CAAACTCTTTTTTCCCATGA+TGG + chr6_3:2308823-2308842 None:intergenic 35.0%
! CAAGGGGTTGAATAAACTTT+TGG + chr6_3:2309018-2309037 None:intergenic 35.0%
GATAAGGATGTTTGTACAAG+GGG + chr6_3:2309034-2309053 None:intergenic 35.0%
TTAAGCGAATTTCATGGTCT+TGG + chr6_3:2309610-2309629 None:intergenic 35.0%
AAAAGTAGTGAATATTGCGG+AGG + chr6_3:2309645-2309664 None:intergenic 35.0%
!! GGAAGGTGTCTTATCTAATT+TGG + chr6_3:2309722-2309741 None:intergenic 35.0%
ATTGGATTGCAACAGATTAG+AGG + chr6_3:2309805-2309824 None:intergenic 35.0%
!! GGAGCAAAATTTTAAGTGGA+TGG + chr6_3:2309828-2309847 None:intergenic 35.0%
ATATAAACTAGCAATTGGGG+TGG - chr6_3:2310035-2310054 Msa0933700:intron 35.0%
! TGACAACATGAGAGTGTATA+GGG + chr6_3:2310237-2310256 None:intergenic 35.0%
TCATGTTGTCACTATACTTG+CGG - chr6_3:2310246-2310265 Msa0933700:intron 35.0%
ATCTTTCCAAGATCTACTCT+TGG + chr6_3:2310352-2310371 None:intergenic 35.0%
ATAAAACCTAGTTTCAAGCC+AGG - chr6_3:2310426-2310445 Msa0933700:intron 35.0%
! ATTATACCTGGCTTGAAACT+AGG + chr6_3:2310435-2310454 None:intergenic 35.0%
! ACTGTATGTTTTTCTCCCTT+TGG - chr6_3:2310467-2310486 Msa0933700:intron 35.0%
!!! GTTTTGCTTTTGGTTCATCA+TGG - chr6_3:2310502-2310521 Msa0933700:intron 35.0%
!!! CTTTTGGTTCATCATGGTTT+TGG - chr6_3:2310508-2310527 Msa0933700:intron 35.0%
!! TTTTGGAAGGTATGCAATTC+GGG - chr6_3:2310545-2310564 Msa0933700:intron 35.0%
ATGGAATGTACCTTCCAAAA+TGG - chr6_3:2310572-2310591 Msa0933700:intron 35.0%
! TCAACTCTTTCCATTTTGGA+AGG + chr6_3:2310585-2310604 None:intergenic 35.0%
GCACCACAAAAAGAAAGAAA+AGG - chr6_3:2310677-2310696 Msa0933700:intron 35.0%
TCCCCTACACAAAAAACAAT+TGG - chr6_3:2311105-2311124 Msa0933700:intron 35.0%
AAGGTGCAAAAATGCAATCT+TGG + chr6_3:2311411-2311430 None:intergenic 35.0%
ACTGTATCTTCTCTTTGATG+AGG + chr6_3:2311523-2311542 None:intergenic 35.0%
AGGATTAAAGATCCAGTGTA+TGG - chr6_3:2311571-2311590 Msa0933700:intron 35.0%
TTTGAGCAATGATTCTTGTG+GGG - chr6_3:2311753-2311772 Msa0933700:CDS 35.0%
TATTGAGGCATATGTCTTGT+TGG + chr6_3:2311907-2311926 None:intergenic 35.0%
ACAATAGGAAGCCCTAAATT+TGG + chr6_3:2311960-2311979 None:intergenic 35.0%
!! CAATCTTCTTTGATGGTGCA+TGG - chr6_3:2308543-2308562 Msa0933700:three_prime_UTR 40.0%
!! ATCTTCTTTGATGGTGCATG+GGG - chr6_3:2308545-2308564 Msa0933700:three_prime_UTR 40.0%
! AAAAGAGGAAGAAGCGTGAA+TGG - chr6_3:2308603-2308622 Msa0933700:three_prime_UTR 40.0%
! AAAGAGGAAGAAGCGTGAAT+GGG - chr6_3:2308604-2308623 Msa0933700:three_prime_UTR 40.0%
TCAACCTCAGCTATAACAGA+AGG + chr6_3:2308864-2308883 None:intergenic 40.0%
! AGCTGAGGTTGATTAATGTG+AGG - chr6_3:2308872-2308891 Msa0933700:three_prime_UTR 40.0%
! GCTGAGGTTGATTAATGTGA+GGG - chr6_3:2308873-2308892 Msa0933700:three_prime_UTR 40.0%
AACAGTCAAACAGAGAGAGT+GGG - chr6_3:2308908-2308927 Msa0933700:three_prime_UTR 40.0%
CAATAAAGGTGGACAAGCAT+GGG + chr6_3:2308942-2308961 None:intergenic 40.0%
CATGCTTGTCCACCTTTATT+GGG - chr6_3:2308941-2308960 Msa0933700:three_prime_UTR 40.0%
ACTACGGTGTGGTGTATATT+TGG + chr6_3:2309696-2309715 None:intergenic 40.0%
TAATTTGGCTAACTACGGTG+TGG + chr6_3:2309707-2309726 None:intergenic 40.0%
TGAGAGCCGGTTATCTTAAA+AGG + chr6_3:2309989-2310008 None:intergenic 40.0%
! TTTCTGGTGGTGAGAAAGAA+TGG + chr6_3:2310202-2310221 None:intergenic 40.0%
! GTGACAACATGAGAGTGTAT+AGG + chr6_3:2310238-2310257 None:intergenic 40.0%
GAAAGATCTAGCCTAGCTAA+GGG - chr6_3:2310365-2310384 Msa0933700:intron 40.0%
! TTTGGAAGGTATGCAATTCG+GGG - chr6_3:2310546-2310565 Msa0933700:intron 40.0%
AGAAACTTCCACATGTCTAG+TGG - chr6_3:2310611-2310630 Msa0933700:intron 40.0%
TCACATGGAAGTGGCTAAAA+AGG + chr6_3:2310657-2310676 None:intergenic 40.0%
! TTCTTTTTGTGGTGCTCACA+TGG + chr6_3:2310672-2310691 None:intergenic 40.0%
TGGAGAATTTGAGTAGCTTG+AGG + chr6_3:2311353-2311372 None:intergenic 40.0%
TAGCTTATGAAGCTGAAGCA+AGG - chr6_3:2311551-2311570 Msa0933700:intron 40.0%
CATCAAGGTTCTTCTTCTCT+TGG - chr6_3:2311685-2311704 Msa0933700:intron 40.0%
! GATTGATTCCAACGCTTCAC+AGG - chr6_3:2308366-2308385 Msa0933700:three_prime_UTR 45.0%
TTTGTGTACCTGTGAAGCGT+TGG + chr6_3:2308377-2308396 None:intergenic 45.0%
! AAGAAGCGTGAATGGGTTTC+TGG - chr6_3:2308611-2308630 Msa0933700:three_prime_UTR 45.0%
! AGAAGCGTGAATGGGTTTCT+GGG - chr6_3:2308612-2308631 Msa0933700:three_prime_UTR 45.0%
!! TAAAGGGCACTTTCCCATCA+TGG - chr6_3:2308806-2308825 Msa0933700:three_prime_UTR 45.0%
!! AAAGGGCACTTTCCCATCAT+GGG - chr6_3:2308807-2308826 Msa0933700:three_prime_UTR 45.0%
CAGAAGGTGACGTTTAGCAT+GGG + chr6_3:2308848-2308867 None:intergenic 45.0%
ACAGAAGGTGACGTTTAGCA+TGG + chr6_3:2308849-2308868 None:intergenic 45.0%
GTCACCTTCTGTTATAGCTG+AGG - chr6_3:2308857-2308876 Msa0933700:three_prime_UTR 45.0%
GAACAGTCAAACAGAGAGAG+TGG - chr6_3:2308907-2308926 Msa0933700:three_prime_UTR 45.0%
ACAGTCAAACAGAGAGAGTG+GGG - chr6_3:2308909-2308928 Msa0933700:three_prime_UTR 45.0%
CCAATAAAGGTGGACAAGCA+TGG + chr6_3:2308943-2308962 None:intergenic 45.0%
CCATGCTTGTCCACCTTTAT+TGG - chr6_3:2308940-2308959 Msa0933700:three_prime_UTR 45.0%
!! TTTCTTGAAGGGCACTAACG+AGG + chr6_3:2309413-2309432 None:intergenic 45.0%
AGTAGTGAATATTGCGGAGG+TGG + chr6_3:2309642-2309661 None:intergenic 45.0%
CTGAGATTGTAGTTGAGAGC+CGG + chr6_3:2310002-2310021 None:intergenic 45.0%
! CAACATGAGAGTGTATAGGG+AGG + chr6_3:2310234-2310253 None:intergenic 45.0%
AAGATCTACTCTTGGAGAGG+GGG + chr6_3:2310344-2310363 None:intergenic 45.0%
CAAGATCTACTCTTGGAGAG+GGG + chr6_3:2310345-2310364 None:intergenic 45.0%
CCAAGATCTACTCTTGGAGA+GGG + chr6_3:2310346-2310365 None:intergenic 45.0%
TCCAAGATCTACTCTTGGAG+AGG + chr6_3:2310347-2310366 None:intergenic 45.0%
GGAAAGATCTAGCCTAGCTA+AGG - chr6_3:2310364-2310383 Msa0933700:intron 45.0%
! TACCTTTTCCACCCTTAGCT+AGG + chr6_3:2310379-2310398 None:intergenic 45.0%
ACTTCCACATGTCTAGTGGT+AGG - chr6_3:2310615-2310634 Msa0933700:intron 45.0%
AGAAACTTGCAAGCAGCACA+TGG + chr6_3:2311373-2311392 None:intergenic 45.0%
ACTTATGAGGCTCTTCTGGT+GGG + chr6_3:2311438-2311457 None:intergenic 45.0%
AACTTATGAGGCTCTTCTGG+TGG + chr6_3:2311439-2311458 None:intergenic 45.0%
GCAAACTTATGAGGCTCTTC+TGG + chr6_3:2311442-2311461 None:intergenic 45.0%
GATCCAGTGTATGGTTGTGT+TGG - chr6_3:2311580-2311599 Msa0933700:intron 45.0%
GGTTCTTCTTCTCTTGGTCT+TGG - chr6_3:2311691-2311710 Msa0933700:intron 45.0%
TGTGGGGATTCCTACAACAA+AGG - chr6_3:2311769-2311788 Msa0933700:CDS 45.0%
GTAGTGAATATGGACGTTGC+AGG - chr6_3:2311858-2311877 Msa0933700:intron 45.0%
! TGAATGGGTTTCTGGGTAGG+TGG - chr6_3:2308619-2308638 Msa0933700:three_prime_UTR 50.0%
GGAAGAAGCAGGGAAAGAGA+AGG - chr6_3:2308640-2308659 Msa0933700:three_prime_UTR 50.0%
!! GAAAGTGCCCTTTACAGCCA+TGG + chr6_3:2308800-2308819 None:intergenic 50.0%
!! GAGGCAAAGAGCATTTCTGG+TGG + chr6_3:2310215-2310234 None:intergenic 50.0%
!! AGGGAGGCAAAGAGCATTTC+TGG + chr6_3:2310218-2310237 None:intergenic 50.0%
CCCTCTCCAAGAGTAGATCT+TGG - chr6_3:2310343-2310362 Msa0933700:intron 50.0%
AGATCTAGCCTAGCTAAGGG+TGG - chr6_3:2310368-2310387 Msa0933700:intron 50.0%
AGCCTAGCTAAGGGTGGAAA+AGG - chr6_3:2310374-2310393 Msa0933700:intron 50.0%
GGTATGCAATTCGGGGTGAA+TGG - chr6_3:2310553-2310572 Msa0933700:intron 50.0%
CACACCTACCACTAGACATG+TGG + chr6_3:2310622-2310641 None:intergenic 50.0%
TTGTGGTGCTCACATGGAAG+TGG + chr6_3:2310666-2310685 None:intergenic 50.0%
GGCTCTTCTGGTGGGAAATA+AGG + chr6_3:2311430-2311449 None:intergenic 50.0%
GCTCCAACACAACCATACAC+TGG + chr6_3:2311586-2311605 None:intergenic 50.0%
! GCGTGAATGGGTTTCTGGGT+AGG - chr6_3:2308616-2308635 Msa0933700:three_prime_UTR 55.0%
TCTGGGTAGGTGGAAGAAGC+AGG - chr6_3:2308629-2308648 Msa0933700:three_prime_UTR 55.0%
CTGGGTAGGTGGAAGAAGCA+GGG - chr6_3:2308630-2308649 Msa0933700:three_prime_UTR 55.0%
AGCAGGGAAAGAGAAGGCAG+TGG - chr6_3:2308646-2308665 Msa0933700:three_prime_UTR 55.0%
TACTCTTGGAGAGGGGGAGA+GGG + chr6_3:2310338-2310357 None:intergenic 55.0%
CTACTCTTGGAGAGGGGGAG+AGG + chr6_3:2310339-2310358 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 2308346 2312040 2308346 ID=Msa0933700;Name=Msa0933700
chr6_3 mRNA 2308346 2312040 2308346 ID=Msa0933700-mRNA-1;Parent=Msa0933700;Name=Msa0933700-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0.33|0.5|0.5|0.66|0.5|4|705|80
chr6_3 exon 2308346 2309048 2308346 ID=Msa0933700-mRNA-1:exon:921;Parent=Msa0933700-mRNA-1
chr6_3 exon 2309754 2309832 2309754 ID=Msa0933700-mRNA-1:exon:920;Parent=Msa0933700-mRNA-1
chr6_3 exon 2311753 2311876 2311753 ID=Msa0933700-mRNA-1:exon:919;Parent=Msa0933700-mRNA-1
chr6_3 exon 2311999 2312040 2311999 ID=Msa0933700-mRNA-1:exon:918;Parent=Msa0933700-mRNA-1
chr6_3 CDS 2311999 2312040 2311999 ID=Msa0933700-mRNA-1:cds;Parent=Msa0933700-mRNA-1
chr6_3 CDS 2311753 2311876 2311753 ID=Msa0933700-mRNA-1:cds;Parent=Msa0933700-mRNA-1
chr6_3 CDS 2309756 2309832 2309756 ID=Msa0933700-mRNA-1:cds;Parent=Msa0933700-mRNA-1
chr6_3 three_prime_UTR 2309754 2309755 2309754 ID=Msa0933700-mRNA-1:three_prime_utr;Parent=Msa0933700-mRNA-1
chr6_3 three_prime_UTR 2308346 2309048 2308346 ID=Msa0933700-mRNA-1:three_prime_utr;Parent=Msa0933700-mRNA-1
Gene Sequence

>Msa0933700

ATGAACAACAGATCAATATCGATTGATTCCAACGCTTCACAGGAAAATAAAGAATTCGAAATTGAAAGAACAATTCAATCTTCTTTGATGGTGCATGGGGTATTTGCAACCATGATTAAGAAGGTTGTAGATGAGAAAAGAGGAAGAAGCGTGAATGGGTTTCTGGGTATGCAATTCGGGGTGAATGGAATGTACCTTCCAAAATGGAAAGAGTTGAAAATTGAGAAACTTCCACATGTCTAG
Protein sequence

>Msa0933700

MNNRSISIDSNASQENKEFEIERTIQSSLMVHGVFATMIKKVVDEKRGRSVNGFLGMQFGVNGMYLPKWKELKIEKLPHV*