Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0935530 | A0A072U5H4 | 96.287 | 1589 | 57 | 1 | 1 | 1587 | 1 | 1589 | 0.0 | 3053 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0935530 | Msa0972450 | 0.972085 | 3.408125e-134 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0935530 | MtrunA17_Chr6g0451341 | 96.287 | 1589 | 57 | 1 | 1 | 1587 | 1 | 1589 | 0.0 | 3053 |
| Msa0935530 | MtrunA17_Chr8g0386831 | 34.014 | 147 | 91 | 4 | 39 | 179 | 99 | 245 | 4.30e-17 | 87.0 |
| Msa0935530 | MtrunA17_Chr5g0397411 | 33.333 | 141 | 90 | 3 | 39 | 175 | 96 | 236 | 4.50e-16 | 84.0 |
| Msa0935530 | MtrunA17_Chr6g0474401 | 32.331 | 133 | 86 | 3 | 47 | 175 | 12 | 144 | 3.67e-14 | 74.7 |
| Msa0935530 | MtrunA17_Chr1g0156791 | 32.450 | 151 | 92 | 5 | 254 | 397 | 1 | 148 | 3.13e-13 | 75.1 |
| Msa0935530 | MtrunA17_Chr1g0156791 | 41.935 | 124 | 46 | 5 | 1125 | 1248 | 599 | 696 | 9.75e-11 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0935530 | AT3G48050.1 | 51.880 | 798 | 337 | 18 | 38 | 805 | 46 | 826 | 0.0 | 660 |
| Msa0935530 | AT3G48050.2 | 51.880 | 798 | 337 | 18 | 38 | 805 | 46 | 826 | 0.0 | 660 |
| Msa0935530 | AT3G48060.1 | 51.439 | 799 | 339 | 19 | 38 | 805 | 46 | 826 | 0.0 | 648 |
| Msa0935530 | AT3G48060.1 | 40.830 | 578 | 279 | 19 | 1043 | 1574 | 1046 | 1606 | 1.03e-97 | 348 |
| Msa0935530 | AT3G48060.3 | 51.439 | 799 | 339 | 19 | 38 | 805 | 46 | 826 | 0.0 | 648 |
| Msa0935530 | AT3G48060.3 | 40.830 | 578 | 279 | 19 | 1043 | 1574 | 1046 | 1606 | 1.03e-97 | 348 |
| Msa0935530 | AT3G48060.2 | 51.314 | 799 | 340 | 19 | 38 | 805 | 47 | 827 | 0.0 | 647 |
| Msa0935530 | AT3G48060.2 | 40.830 | 578 | 279 | 19 | 1043 | 1574 | 1047 | 1607 | 9.47e-98 | 348 |
| Msa0935530 | AT4G11560.1 | 36.220 | 127 | 76 | 3 | 54 | 175 | 141 | 267 | 1.04e-15 | 83.2 |
| Msa0935530 | AT4G11560.2 | 36.220 | 127 | 76 | 3 | 54 | 175 | 141 | 267 | 1.21e-15 | 82.4 |
Find 487 sgRNAs with CRISPR-Local
Find 665 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAACCAGTTCTTCAAC+TGG | 0.416688 | 6_3:-6113349 | Msa0935530:CDS |
| AAAAGGAAGGAACCTGATGG+CGG | 0.689775 | 6_3:-6110778 | Msa0935530:CDS |
| AAAATCCATTGCGGAGTAAG+GGG | 0.556892 | 6_3:-6111905 | Msa0935530:CDS |
| AAAATGGGGAAGTCAGAGTT+TGG | 0.408671 | 6_3:-6110941 | Msa0935530:CDS |
| AAACAAAACTCATCTGCAAG+AGG | 0.613981 | 6_3:+6110241 | None:intergenic |
| AAACAGCCTTCATTATCCAG+TGG | 0.636195 | 6_3:-6111561 | Msa0935530:CDS |
| AAACGGTGACTACAGTGGTC+GGG | 0.565568 | 6_3:+6116779 | None:intergenic |
| AAAGAGAGAGCGAGGAGATC+AGG | 0.345060 | 6_3:-6114802 | Msa0935530:CDS |
| AAAGGAGGCGAGTCCTGGGG+AGG | 0.646644 | 6_3:+6115396 | None:intergenic |
| AAAGGAGTCTCATGCAGAGG+CGG | 0.538075 | 6_3:-6112801 | Msa0935530:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCTATAGATAGATCGATGA+GGG | + | chr6_3:6109946-6109965 | None:intergenic | 30.0% | |
| AATCTATAGATAGATCGATG+AGG | + | chr6_3:6109947-6109966 | None:intergenic | 30.0% | |
| CGATCGAATTGAAATGAATT+AGG | + | chr6_3:6110070-6110089 | None:intergenic | 30.0% | |
| TGTGTTTGATTACCTTAATC+TGG | - | chr6_3:6110604-6110623 | Msa0935530:intron | 30.0% | |
| AAAACTTCTCATCCAGATTA+AGG | + | chr6_3:6110619-6110638 | None:intergenic | 30.0% | |
| AAACATGAATACAACAGGTT+TGG | + | chr6_3:6111023-6111042 | None:intergenic | 30.0% | |
| TTATTGAAACTGTGTTCTGA+TGG | - | chr6_3:6111133-6111152 | Msa0935530:CDS | 30.0% | |
| GTTATGGTTAAAACAAACCT+TGG | - | chr6_3:6111261-6111280 | Msa0935530:CDS | 30.0% | |
| AAAGCAAATTGAAGCTCAAA+AGG | + | chr6_3:6111332-6111351 | None:intergenic | 30.0% | |
| CTAATTGGCAAAAACAGTAA+CGG | + | chr6_3:6111524-6111543 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 6109764 | 6117337 | 6109764 | ID=Msa0935530;Name=Msa0935530 |
| chr6_3 | mRNA | 6109764 | 6117337 | 6109764 | ID=Msa0935530-mRNA-1;Parent=Msa0935530;Name=Msa0935530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=471|1|1|1|0.66|0.5|4|668|1587 |
| chr6_3 | exon | 6109764 | 6110388 | 6109764 | ID=Msa0935530-mRNA-1:exon:1892;Parent=Msa0935530-mRNA-1 |
| chr6_3 | exon | 6110683 | 6115003 | 6110683 | ID=Msa0935530-mRNA-1:exon:1891;Parent=Msa0935530-mRNA-1 |
| chr6_3 | exon | 6115089 | 6115460 | 6115089 | ID=Msa0935530-mRNA-1:exon:1890;Parent=Msa0935530-mRNA-1 |
| chr6_3 | exon | 6116753 | 6117337 | 6116753 | ID=Msa0935530-mRNA-1:exon:1889;Parent=Msa0935530-mRNA-1 |
| chr6_3 | five_prime_UTR | 6116867 | 6117337 | 6116867 | ID=Msa0935530-mRNA-1:five_prime_utr;Parent=Msa0935530-mRNA-1 |
| chr6_3 | CDS | 6116753 | 6116866 | 6116753 | ID=Msa0935530-mRNA-1:cds;Parent=Msa0935530-mRNA-1 |
| chr6_3 | CDS | 6115089 | 6115460 | 6115089 | ID=Msa0935530-mRNA-1:cds;Parent=Msa0935530-mRNA-1 |
| chr6_3 | CDS | 6110726 | 6115003 | 6110726 | ID=Msa0935530-mRNA-1:cds;Parent=Msa0935530-mRNA-1 |
| chr6_3 | three_prime_UTR | 6110683 | 6110725 | 6110683 | ID=Msa0935530-mRNA-1:three_prime_utr;Parent=Msa0935530-mRNA-1 |
| chr6_3 | three_prime_UTR | 6109764 | 6110388 | 6109764 | ID=Msa0935530-mRNA-1:three_prime_utr;Parent=Msa0935530-mRNA-1 |
| Gene Sequence |
>Msa0935530 ATGTATGGGTTTGGTGGTGAGAAGAAGTGGAAACACAATCGGCACATGTGGCCTGTACCTTCTAACCCGACCACTGTAGTCACCGTTTCTTCACCGTCAAAATTTATATGCAAAGATGGACGCAAGATTCGTGTTGGTGACTGTGCCCTATTCAAACCTCCCCAGGACTCGCCTCCTTTTATTGGAATAATTCGCAAGTTAATATTTAATAAAGAAGAAAGTCCAAGTTTAGAAGTGAATTGGTTTTACCGACCTGCTGATTTGAAGCTCTCTAAAGGGATTGCAGTGGAAGCTGCACCAAACGAAGTCTTCTACTCCTTTCACAAGGATGAGACACATGCTGCATCATTACTCCATCCTTGTAAAGTCGCATTTCTCCGTAAAGGTGTTGAACTTCCTTCAGGGATATCCGCATTTGTTTGTAGACGAGTGTACGACATTGACAACAATTGTTTACGGTGGCTAACTGATAAGGACTTCGTTAATGATCAGCAGGAAGAAGTTGACCAACTATTAGACAAGACAAAACTAGAAATGCATGGGGCTGTGCAGTCAGGAGGCCGTTCTCCAAAACCCTTGAATGGTCCAACGTCAACACAGTCATTGAAATCTAGTTCTGATAACATTCAAAATAGTTCTTCCTTTGGTGTACAGGGTAAGGGAAAAAAGAGAGAGCGAGGAGATCAGGGATCTGATTCATCTAAAAAAGAGCGATTATTTAAGGTAGAAGTTGGAGATTCTGGTCAGTTTAGACTGGAGATGCTAAGGACCGAGATTGCTAGAATCACTGAAAAAGGCGGGCTTGTGGACTTTGAAGGAGTTGAAAAATTTGTACAGCTTATGCAACCTGATAGTGCTGATAAGAAAATTGATTTGTCTGGGAGAACGATGCTTGTTGACGTCATATCGCTCACGGAACATTATGACTGTCTTGGCTGGTTTGTACAGCTTCGTGGTTTGCCTGTGTTAGATGAATGGCTACAAGAGGTCCATAAGGGGAAAATTGGCGATGGTAATGTTGATAAGTCTGAAGAGTTTTTGTTAGCTTTACTCCGTGCATTGGATAAGCTTCCTGTGAACCTTCATGCACTACAAACCTGCAATGTTGGAAAATCTGTTAATAATTTACGCAGCCACAAAAATTCTGAAATTCAGAGGAAAGCCAGGAGTTTGGTAGACACATGGAAAAAGCGTGTTGAAGCTGAAATGAATATGACTGATTCAAAACCTGGTTCAACCCGTGCTGTTTCTTGGCCCACAAAGCCATCACCGTCAGATGTTTCTCACTCTGGAAATAGGAGAACCGGAGGATCCTCTGAGAATGTTGCGAAGAGCTCTCCTATTCAACCTTCAGCATCCAAAAATTCCCAATCTAAACATAATTCTGGAGAAGTACTATCCAAGTTCTCTTCATCCCCTGGCTCAACTAAATCAATGACTGCCTCAGTAGGCAGCAACACAAAAGATCAAAATGTTAAACTCATTGTTGGTGCAACGACATCAGATCTACCTCTGACACCTATCAAGGAGGAGAGGAGCAGCAGTTCTAGTCAATCTCAAAACAACAGCAGATCTTGTTCTAGTGAACATGCGAAGAGCTCCACTGCTGGGTCAATAAGTGGGAGCAAAGTTCCTGGTAGTGCATCCCGATCTCGAAAGTCGAGTAATGGTATACATGCAGCTGGCGTTGCTGTAGTTCTGAAGGATCATAGCTCTGCCAAGAATTCAACAAGAAATTCATCTTCTGATAAAGTTTCACCGACCAGAATGTCCCATGAAAAACCATCTGATCAGCCCCTTACTGATCAAGGGCATAATCAGCGATTGATTGTGAGGTTGCCGAACACTGGGCGCAGTCCTTCCAGGGGAGCTAGTGGGGGCACTTTTGAAGAACCTGCTATTACATGTGGCAAAACATCACCTCCTACCGACAAGAATGATAATCAGGATAGGAGAGTTAAAGCCAAAACTGATTGTTTGCAAACCCATGTGTCGAATGTGATGAATGATGCATCCAATGCTAAGGAAATTACTGGTTGTGATGAAGCTAAGTGCTCTCCACGAGTGGATGAACGGTGCAGGGCTAATGAAGATGGTGATAAAGTAGCAGAAACGTCAAAAACAACCAGTTCTTCAACTGGATTTGTTTCCAGATCAGGGCAGACTTATGATGCTTCTTTAAGCCCCATGAATGCATTAGTTGAGAGCTGTGTGAAATTTTCCGAAGCAAGTTCCTCTGTGTCCCCTGGAGATGATGGTATGAATCTGCTTGCTTCTGTGGCTGCTGGAGAAATGTCTAGATGTGAAAATGTCTCACCTCTGCCTTCACCCGAGAGGAAGTCTCCTGCAGCTGATGAATCAAGCTCAGGGAATGATCGACATTCTTTTGAAGCAGCTGGTCGCACGCAGAGACAGTCTGATGGTGGGGCTACCGGAGAGCATCCTGTGAATACTTTGCAATTCAAAAATAATTCTAGGCATCTGGTGACTTCAGTATCGCGTGATTTTTCAGATGGTGAAGCCGTTTCATCTAGCTGTGTGGAGAAAACTGGTGATGGTAAAACTCAATTAAATTTCTCTACTACGGACACGATACAGAATGCTGGAGGTCCATGCTTACGGCCTGACACCAAAGAGGATACGTCTGAAACTGTATTTCCAGGCAGAAAGGAGTCTCATGCAGAGGCGGGAGGCGCAGAGGGTTTTCACGAGAGGAGGGAACTGGGAACTCAATGGCCTAAAAGCAGCAGTAGTCCAGGCTCTAAAAAATCGAGGACTTCCTCTTTTGATGATGATCAGAAGACTGATAACATGGATGGCGGTCTCACAGAGCACGGGAAAATGTTAGTTTCAGAAACCGTTGCAAGTGGTATGATTGAAAATGAACCAGGTAAAAAATCCCCCGAGTTATCTTCAGGTGTAGATAGTAAGAGCCAGTTCAGTGCGGAGAAAATGATGGGTAGTGTGCCAATGCAAAAAGGCTCTCCAGTTGCTGATACCTGTGAGTCCATAGATGTGAAAAGGGAGGATGTTATGTTACCCGCTTCTGGTAGTGCTTTGAAAAGGGAGGATGTTGCTAATAATGTTATGGCAGTTGAGATCAAGCCTGCTGAGAAACAAATGGATCTGGATTCAGCAGTTGCAGATGGTGTTGATGAGCGTTGTGAAGGGAATTCTGTTAAAAAAGAGTTCATTGGCGGTTCATCTGTTCCCTCAGATATACCAATTACTTCTGAAAAGGAGAGCGACGTGCCCGAAACATGTGATTCTAATATAGAAGGAAAGAAATCTGTAGTTTCTGCAGAGTTGAATGCGGTCAACACTAATCCATCCACAATTGCTAGTGGTTCTGATGCAGCTGTAAAACTGGACTTTGATTTAAATGAGGGATACCCAGTTGAAGATGCAGGCCAAGGGGATATTGTTAGACAGGAAGATCCTACCAGTTCATCTGCAGTTCATGTTCCTTGCCCATTGCCTTTTCCTATTCCATCCCTCTCTGGGGCCTTCCATGCTTCTATTACAGTGGCTTCTGCTACCAAAGGACCAGTTGTTCTACCAGAAAATCCATTGCGGAGTAAGGGGGAACTTGGATGGAAAGGTTCAGCTGCTACAAGTGCATTCCGGCCAGCTGAACCTAGGAAAAATGCTGAGATGCCATCAAATACTGGTGATATTCCTTCCGTTGAAACAACATCAGTCAAACAAGGCCGTGCTCCTTTAGATTTTGATTTAAATGTGGCAGATGAAATAGGTTTTGAGGACGTTGCTTACCGGGGTTCCTTAGAGTCTGGAGCACATGACCGCAGTGCTGTGGGACTTGATCTTGATCTTAACAGGTTAGATGAGACTCCTGAAGCTGGATCTTTCTCTATGGGTAAAATGGATATTCCTTCTTTACCAAGTAAACAGCCTTCATTATCCAGTGGGTTATCTAATGGCGGAAGTGTCTCAAGAGACTTTGATTTAAATAATGGGCCAGGCCTTGACGAAGTTAGCACTGAAGTCCCTGCTCGTAGTCTGCAGATGAAAGGTGCTGTGCCATTTTCAAATTCTGTTCATGGCACCAGAACAAATAACGCTGAATTTGGGAACTATTCTTCATGGTTTCCTCCAGGTAATTCGTATTCTGCAATTACTGTCCCACCACTTCTGCCTGGTAGAGGGGAACAGAGCTATGTTGGTGCTTCTGGACCACAGCGGATCATAGGTTCGACAGGTAGCTCACCCTTTAGTCCTGAAATGTACAGAGGGCCAGTACTTTCATCCTCTCCTGCTGTTGCATATCCTCCTACAACCCCCTTTCCATATCCAGGATTTCCATTTGAAACCAATTTTCCATTATCTTCAAACGCCTTTTCTGGTTGTTCAACACCATTTATGGATTCATCAACTGTTGGTGGACTTTGCTTTCCTACTATGCCCTCGCAGCCAGTTGGGCCTGGAGGCATTGTTTCATCTACATATCCCCGTCCTTATGTTATGAGTCTTCCAGGCAGTACAAGTAACGTAATTCCCGACAGCAGAAAATGGGGAAGTCAGAGTTTGGATCTTAATTCAGGCCCTGGTGGTACAGACGTCGAGCGCAGAGACGACAGATTGCCTTCAGGGTTGAGGCAGATGCCTGTCCCCAGTCCACAAGCCTTGATGGAGGATCACTTGAAGATGTTTCAGATGGCCGGTGCATTAAAAAGGAAGGAACCTGATGGCGGTTGGGATGGAACTGATAGATTCAGTTACAAACATCCCCCCTCATGGCAGTAG |
| Protein sequence |
>Msa0935530 MYGFGGEKKWKHNRHMWPVPSNPTTVVTVSSPSKFICKDGRKIRVGDCALFKPPQDSPPFIGIIRKLIFNKEESPSLEVNWFYRPADLKLSKGIAVEAAPNEVFYSFHKDETHAASLLHPCKVAFLRKGVELPSGISAFVCRRVYDIDNNCLRWLTDKDFVNDQQEEVDQLLDKTKLEMHGAVQSGGRSPKPLNGPTSTQSLKSSSDNIQNSSSFGVQGKGKKRERGDQGSDSSKKERLFKVEVGDSGQFRLEMLRTEIARITEKGGLVDFEGVEKFVQLMQPDSADKKIDLSGRTMLVDVISLTEHYDCLGWFVQLRGLPVLDEWLQEVHKGKIGDGNVDKSEEFLLALLRALDKLPVNLHALQTCNVGKSVNNLRSHKNSEIQRKARSLVDTWKKRVEAEMNMTDSKPGSTRAVSWPTKPSPSDVSHSGNRRTGGSSENVAKSSPIQPSASKNSQSKHNSGEVLSKFSSSPGSTKSMTASVGSNTKDQNVKLIVGATTSDLPLTPIKEERSSSSSQSQNNSRSCSSEHAKSSTAGSISGSKVPGSASRSRKSSNGIHAAGVAVVLKDHSSAKNSTRNSSSDKVSPTRMSHEKPSDQPLTDQGHNQRLIVRLPNTGRSPSRGASGGTFEEPAITCGKTSPPTDKNDNQDRRVKAKTDCLQTHVSNVMNDASNAKEITGCDEAKCSPRVDERCRANEDGDKVAETSKTTSSSTGFVSRSGQTYDASLSPMNALVESCVKFSEASSSVSPGDDGMNLLASVAAGEMSRCENVSPLPSPERKSPAADESSSGNDRHSFEAAGRTQRQSDGGATGEHPVNTLQFKNNSRHLVTSVSRDFSDGEAVSSSCVEKTGDGKTQLNFSTTDTIQNAGGPCLRPDTKEDTSETVFPGRKESHAEAGGAEGFHERRELGTQWPKSSSSPGSKKSRTSSFDDDQKTDNMDGGLTEHGKMLVSETVASGMIENEPGKKSPELSSGVDSKSQFSAEKMMGSVPMQKGSPVADTCESIDVKREDVMLPASGSALKREDVANNVMAVEIKPAEKQMDLDSAVADGVDERCEGNSVKKEFIGGSSVPSDIPITSEKESDVPETCDSNIEGKKSVVSAELNAVNTNPSTIASGSDAAVKLDFDLNEGYPVEDAGQGDIVRQEDPTSSSAVHVPCPLPFPIPSLSGAFHASITVASATKGPVVLPENPLRSKGELGWKGSAATSAFRPAEPRKNAEMPSNTGDIPSVETTSVKQGRAPLDFDLNVADEIGFEDVAYRGSLESGAHDRSAVGLDLDLNRLDETPEAGSFSMGKMDIPSLPSKQPSLSSGLSNGGSVSRDFDLNNGPGLDEVSTEVPARSLQMKGAVPFSNSVHGTRTNNAEFGNYSSWFPPGNSYSAITVPPLLPGRGEQSYVGASGPQRIIGSTGSSPFSPEMYRGPVLSSSPAVAYPPTTPFPYPGFPFETNFPLSSNAFSGCSTPFMDSSTVGGLCFPTMPSQPVGPGGIVSSTYPRPYVMSLPGSTSNVIPDSRKWGSQSLDLNSGPGGTDVERRDDRLPSGLRQMPVPSPQALMEDHLKMFQMAGALKRKEPDGGWDGTDRFSYKHPPSWQ* |
