Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0938030 | sp|Q39131|LAML_ARATH | 56.364 | 110 | 44 | 3 | 2 | 108 | 64 | 172 | 1.11e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0938030 | Q2HW94 | 96.262 | 107 | 4 | 0 | 2 | 108 | 73 | 179 | 1.85e-72 | 222 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0180930 | Msa0938030 | 0.800118 | 1.674267e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0938030 | MtrunA17_Chr6g0453921 | 96.262 | 107 | 4 | 0 | 2 | 108 | 73 | 179 | 3.56e-76 | 222 |
| Msa0938030 | MtrunA17_Chr6g0453801 | 83.099 | 71 | 12 | 0 | 2 | 72 | 70 | 140 | 4.13e-41 | 133 |
| Msa0938030 | MtrunA17_Chr3g0144921 | 44.118 | 102 | 52 | 3 | 2 | 101 | 66 | 164 | 1.52e-19 | 78.6 |
| Msa0938030 | MtrunA17_Chr5g0440791 | 47.500 | 80 | 41 | 1 | 2 | 80 | 20 | 99 | 2.14e-19 | 77.0 |
| Msa0938030 | MtrunA17_Chr5g0440804 | 50.000 | 76 | 37 | 1 | 6 | 80 | 69 | 144 | 2.51e-18 | 75.5 |
| Msa0938030 | MtrunA17_Chr5g0440711 | 51.351 | 74 | 35 | 1 | 6 | 78 | 69 | 142 | 8.80e-18 | 73.6 |
| Msa0938030 | MtrunA17_Chr6g0453791 | 40.566 | 106 | 59 | 2 | 2 | 107 | 66 | 167 | 2.25e-16 | 70.5 |
| Msa0938030 | MtrunA17_Chr4g0001571 | 37.113 | 97 | 52 | 2 | 2 | 91 | 64 | 158 | 3.28e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0938030 | AT5G15350.1 | 56.364 | 110 | 44 | 3 | 2 | 108 | 64 | 172 | 1.13e-40 | 132 |
| Msa0938030 | AT4G12880.2 | 58.730 | 63 | 26 | 0 | 2 | 64 | 26 | 88 | 7.34e-23 | 85.5 |
| Msa0938030 | AT4G12880.1 | 58.730 | 63 | 26 | 0 | 2 | 64 | 64 | 126 | 4.05e-22 | 84.7 |
| Msa0938030 | AT3G01070.1 | 60.000 | 100 | 35 | 4 | 2 | 96 | 62 | 161 | 4.13e-20 | 80.1 |
| Msa0938030 | AT2G27035.1 | 45.570 | 79 | 40 | 2 | 2 | 80 | 64 | 139 | 1.91e-16 | 70.9 |
Find 14 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGATTCATTCATTTATTTA+TGG | 0.202648 | 6_3:-9541956 | Msa0938030:CDS |
| TCACTTCTAGTACATTCATT+TGG | 0.385696 | 6_3:+9542249 | None:intergenic |
| CAGACCATCCACTTCACAAT+TGG | 0.402160 | 6_3:-9542199 | Msa0938030:CDS |
| CCACTTCACAATTGGACAAC+TGG | 0.404662 | 6_3:-9542191 | Msa0938030:CDS |
| GCAACATTCCTTTCTTCTAA+AGG | 0.414486 | 6_3:-9542023 | Msa0938030:CDS |
| AGATTTGTCCTTTAGAAGAA+AGG | 0.430408 | 6_3:+9542015 | None:intergenic |
| CACAATTGGACAACTGGAGC+TGG | 0.466848 | 6_3:-9542185 | Msa0938030:CDS |
| TACTATTTCATCAGTGGCAA+AGG | 0.473899 | 6_3:-9542128 | Msa0938030:CDS |
| TAATGGCGAGTCACGTTCAA+TGG | 0.474693 | 6_3:+9542149 | None:intergenic |
| CGCCATTACTATTTCATCAG+TGG | 0.560423 | 6_3:-9542134 | Msa0938030:CDS |
| ACCACCACCAAAAGCTGCAC+CGG | 0.566963 | 6_3:-9542057 | Msa0938030:CDS |
| TGCCACTGATGAAATAGTAA+TGG | 0.590760 | 6_3:+9542132 | None:intergenic |
| CCAGTTGTCCAATTGTGAAG+TGG | 0.610427 | 6_3:+9542191 | None:intergenic |
| TTGTCCAATTGTGAAGTGGA+TGG | 0.656337 | 6_3:+9542195 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAAATATTTTCTTCTTT+AGG | - | chr6_3:9542113-9542132 | Msa0938030:CDS | 10.0% |
| !!! | ATTAAATATTTTCTTCTTTA+GGG | - | chr6_3:9542114-9542133 | Msa0938030:CDS | 10.0% |
| !! | GTTTGATATATATATTATAA+CGG | - | chr6_3:9542312-9542331 | Msa0938030:intron | 10.0% |
| !!! | AATTAAATATTTTCTTCTTT+AGG | - | chr6_3:9542113-9542132 | Msa0938030:CDS | 10.0% |
| !!! | ATTAAATATTTTCTTCTTTA+GGG | - | chr6_3:9542114-9542133 | Msa0938030:CDS | 10.0% |
| !! | GTTTGATATATATATTATAA+CGG | - | chr6_3:9542312-9542331 | Msa0938030:intron | 10.0% |
| !!! | AATCACCTTTTTTAACAAAT+AGG | + | chr6_3:9542140-9542159 | None:intergenic | 20.0% |
| !! | AAAACTGAAAAATGAAATCA+AGG | + | chr6_3:9542533-9542552 | None:intergenic | 20.0% |
| !!! | AATCACCTTTTTTAACAAAT+AGG | + | chr6_3:9542140-9542159 | None:intergenic | 20.0% |
| !! | AAAACTGAAAAATGAAATCA+AGG | + | chr6_3:9542533-9542552 | None:intergenic | 20.0% |
| ! | AAGTAATGAAAGTCAACATT+TGG | + | chr6_3:9542022-9542041 | None:intergenic | 25.0% |
| ! | GTGATTTATCATTACTCTTA+AGG | - | chr6_3:9542050-9542069 | Msa0938030:CDS | 25.0% |
| ! | TTAAGACTATGACCAAAATT+AGG | + | chr6_3:9542077-9542096 | None:intergenic | 25.0% |
| !!! | AAATTAAGCGCAATTTGTTA+CGG | + | chr6_3:9542361-9542380 | None:intergenic | 25.0% |
| !! | TTTCAGTTTTTGTGTATGAT+AGG | - | chr6_3:9542543-9542562 | Msa0938030:intron | 25.0% |
| ! | GGGATTCATTCATTTATTTA+TGG | - | chr6_3:9542861-9542880 | Msa0938030:intron | 25.0% |
| ! | AAGTAATGAAAGTCAACATT+TGG | + | chr6_3:9542022-9542041 | None:intergenic | 25.0% |
| ! | GTGATTTATCATTACTCTTA+AGG | - | chr6_3:9542050-9542069 | Msa0938030:CDS | 25.0% |
| ! | TTAAGACTATGACCAAAATT+AGG | + | chr6_3:9542077-9542096 | None:intergenic | 25.0% |
| !!! | AAATTAAGCGCAATTTGTTA+CGG | + | chr6_3:9542361-9542380 | None:intergenic | 25.0% |
| !! | TTTCAGTTTTTGTGTATGAT+AGG | - | chr6_3:9542543-9542562 | Msa0938030:intron | 25.0% |
| ! | GGGATTCATTCATTTATTTA+TGG | - | chr6_3:9542861-9542880 | Msa0938030:intron | 25.0% |
| TTCATTTGCAAATTAAGCCA+TGG | + | chr6_3:9541989-9542008 | None:intergenic | 30.0% | |
| ! | TACTCTTAAGGTCCTAATTT+TGG | - | chr6_3:9542062-9542081 | Msa0938030:CDS | 30.0% |
| ! | TAGGGCCTATTTGTTAAAAA+AGG | - | chr6_3:9542132-9542151 | Msa0938030:CDS | 30.0% |
| AATCTTTCAAGCATTCTCAA+GGG | + | chr6_3:9542458-9542477 | None:intergenic | 30.0% | |
| TAATCTTTCAAGCATTCTCA+AGG | + | chr6_3:9542459-9542478 | None:intergenic | 30.0% | |
| TCACTTCTAGTACATTCATT+TGG | + | chr6_3:9542571-9542590 | None:intergenic | 30.0% | |
| ! | AGATTTGTCCTTTAGAAGAA+AGG | + | chr6_3:9542805-9542824 | None:intergenic | 30.0% |
| !!! | ATGCCTATTGTTTTTGCTAT+TGG | - | chr6_3:9542830-9542849 | Msa0938030:intron | 30.0% |
| TTCATTTGCAAATTAAGCCA+TGG | + | chr6_3:9541989-9542008 | None:intergenic | 30.0% | |
| ! | TACTCTTAAGGTCCTAATTT+TGG | - | chr6_3:9542062-9542081 | Msa0938030:CDS | 30.0% |
| ! | TAGGGCCTATTTGTTAAAAA+AGG | - | chr6_3:9542132-9542151 | Msa0938030:CDS | 30.0% |
| AATCTTTCAAGCATTCTCAA+GGG | + | chr6_3:9542458-9542477 | None:intergenic | 30.0% | |
| TAATCTTTCAAGCATTCTCA+AGG | + | chr6_3:9542459-9542478 | None:intergenic | 30.0% | |
| TCACTTCTAGTACATTCATT+TGG | + | chr6_3:9542571-9542590 | None:intergenic | 30.0% | |
| ! | AGATTTGTCCTTTAGAAGAA+AGG | + | chr6_3:9542805-9542824 | None:intergenic | 30.0% |
| !!! | ATGCCTATTGTTTTTGCTAT+TGG | - | chr6_3:9542830-9542849 | Msa0938030:intron | 30.0% |
| TATGTCCTAAGTACACTTGT+TGG | - | chr6_3:9542388-9542407 | Msa0938030:intron | 35.0% | |
| TGCCACTGATGAAATAGTAA+TGG | + | chr6_3:9542688-9542707 | None:intergenic | 35.0% | |
| TACTATTTCATCAGTGGCAA+AGG | - | chr6_3:9542689-9542708 | Msa0938030:intron | 35.0% | |
| ! | TGGTAGATTTTCAACACGAA+CGG | + | chr6_3:9542741-9542760 | None:intergenic | 35.0% |
| !!! | AATGTTGCACTAGCTTTTTC+CGG | + | chr6_3:9542782-9542801 | None:intergenic | 35.0% |
| GCAACATTCCTTTCTTCTAA+AGG | - | chr6_3:9542794-9542813 | Msa0938030:intron | 35.0% | |
| GCACCAATAGCAAAAACAAT+AGG | + | chr6_3:9542836-9542855 | None:intergenic | 35.0% | |
| TATGTCCTAAGTACACTTGT+TGG | - | chr6_3:9542388-9542407 | Msa0938030:intron | 35.0% | |
| TGCCACTGATGAAATAGTAA+TGG | + | chr6_3:9542688-9542707 | None:intergenic | 35.0% | |
| TACTATTTCATCAGTGGCAA+AGG | - | chr6_3:9542689-9542708 | Msa0938030:intron | 35.0% | |
| ! | TGGTAGATTTTCAACACGAA+CGG | + | chr6_3:9542741-9542760 | None:intergenic | 35.0% |
| !!! | AATGTTGCACTAGCTTTTTC+CGG | + | chr6_3:9542782-9542801 | None:intergenic | 35.0% |
| GCAACATTCCTTTCTTCTAA+AGG | - | chr6_3:9542794-9542813 | Msa0938030:intron | 35.0% | |
| GCACCAATAGCAAAAACAAT+AGG | + | chr6_3:9542836-9542855 | None:intergenic | 35.0% | |
| ! | ATTAAGCCATGGATTGAGGA+GGG | + | chr6_3:9541978-9541997 | None:intergenic | 40.0% |
| ! | AATTAAGCCATGGATTGAGG+AGG | + | chr6_3:9541979-9541998 | None:intergenic | 40.0% |
| ! | GCAAATTAAGCCATGGATTG+AGG | + | chr6_3:9541982-9542001 | None:intergenic | 40.0% |
| CTTATTCCACCAAGTAGGAT+CGG | - | chr6_3:9542265-9542284 | Msa0938030:intron | 40.0% | |
| GAATGCCAACAAGTGTACTT+AGG | + | chr6_3:9542396-9542415 | None:intergenic | 40.0% | |
| TTTCAAGCATTCTCAAGGGT+TGG | + | chr6_3:9542454-9542473 | None:intergenic | 40.0% | |
| TTGTCCAATTGTGAAGTGGA+TGG | + | chr6_3:9542625-9542644 | None:intergenic | 40.0% | |
| CGCCATTACTATTTCATCAG+TGG | - | chr6_3:9542683-9542702 | Msa0938030:intron | 40.0% | |
| ! | AGTGGCAAAGGATTTTGCTT+TGG | - | chr6_3:9542701-9542720 | Msa0938030:intron | 40.0% |
| !!! | TTTTTGCTATTGGTGCAGCA+TGG | - | chr6_3:9542840-9542859 | Msa0938030:intron | 40.0% |
| !!! | TTTTGCTATTGGTGCAGCAT+GGG | - | chr6_3:9542841-9542860 | Msa0938030:intron | 40.0% |
| ! | ATTAAGCCATGGATTGAGGA+GGG | + | chr6_3:9541978-9541997 | None:intergenic | 40.0% |
| ! | AATTAAGCCATGGATTGAGG+AGG | + | chr6_3:9541979-9541998 | None:intergenic | 40.0% |
| ! | GCAAATTAAGCCATGGATTG+AGG | + | chr6_3:9541982-9542001 | None:intergenic | 40.0% |
| CTTATTCCACCAAGTAGGAT+CGG | - | chr6_3:9542265-9542284 | Msa0938030:intron | 40.0% | |
| GAATGCCAACAAGTGTACTT+AGG | + | chr6_3:9542396-9542415 | None:intergenic | 40.0% | |
| TTTCAAGCATTCTCAAGGGT+TGG | + | chr6_3:9542454-9542473 | None:intergenic | 40.0% | |
| TTGTCCAATTGTGAAGTGGA+TGG | + | chr6_3:9542625-9542644 | None:intergenic | 40.0% | |
| CGCCATTACTATTTCATCAG+TGG | - | chr6_3:9542683-9542702 | Msa0938030:intron | 40.0% | |
| ! | AGTGGCAAAGGATTTTGCTT+TGG | - | chr6_3:9542701-9542720 | Msa0938030:intron | 40.0% |
| !!! | TTTTTGCTATTGGTGCAGCA+TGG | - | chr6_3:9542840-9542859 | Msa0938030:intron | 40.0% |
| !!! | TTTTGCTATTGGTGCAGCAT+GGG | - | chr6_3:9542841-9542860 | Msa0938030:intron | 40.0% |
| ! | CATGGATTGAGGAGGGAAAT+TGG | + | chr6_3:9541971-9541990 | None:intergenic | 45.0% |
| CAATTTCCCTCCTCAATCCA+TGG | - | chr6_3:9541969-9541988 | Msa0938030:CDS | 45.0% | |
| ! | CTACTTGGTGGAATAAGGCT+TGG | + | chr6_3:9542262-9542281 | None:intergenic | 45.0% |
| !! | CAAGCCTTATTCCACCAAGT+AGG | - | chr6_3:9542260-9542279 | Msa0938030:intron | 45.0% |
| ! | CGATCCTACTTGGTGGAATA+AGG | + | chr6_3:9542267-9542286 | None:intergenic | 45.0% |
| ! | ATCCATGTACCGATCCTACT+TGG | + | chr6_3:9542277-9542296 | None:intergenic | 45.0% |
| AGCATTCTCAAGGGTTGGAT+TGG | + | chr6_3:9542449-9542468 | None:intergenic | 45.0% | |
| CAGACCATCCACTTCACAAT+TGG | - | chr6_3:9542618-9542637 | Msa0938030:intron | 45.0% | |
| CCAGTTGTCCAATTGTGAAG+TGG | + | chr6_3:9542629-9542648 | None:intergenic | 45.0% | |
| CCACTTCACAATTGGACAAC+TGG | - | chr6_3:9542626-9542645 | Msa0938030:intron | 45.0% | |
| TAATGGCGAGTCACGTTCAA+TGG | + | chr6_3:9542671-9542690 | None:intergenic | 45.0% | |
| ! | GGCAAAGGATTTTGCTTTGG+TGG | - | chr6_3:9542704-9542723 | Msa0938030:intron | 45.0% |
| ! | CATGGATTGAGGAGGGAAAT+TGG | + | chr6_3:9541971-9541990 | None:intergenic | 45.0% |
| CAATTTCCCTCCTCAATCCA+TGG | - | chr6_3:9541969-9541988 | Msa0938030:CDS | 45.0% | |
| ! | CTACTTGGTGGAATAAGGCT+TGG | + | chr6_3:9542262-9542281 | None:intergenic | 45.0% |
| !! | CAAGCCTTATTCCACCAAGT+AGG | - | chr6_3:9542260-9542279 | Msa0938030:intron | 45.0% |
| ! | CGATCCTACTTGGTGGAATA+AGG | + | chr6_3:9542267-9542286 | None:intergenic | 45.0% |
| ! | ATCCATGTACCGATCCTACT+TGG | + | chr6_3:9542277-9542296 | None:intergenic | 45.0% |
| AGCATTCTCAAGGGTTGGAT+TGG | + | chr6_3:9542449-9542468 | None:intergenic | 45.0% | |
| CAGACCATCCACTTCACAAT+TGG | - | chr6_3:9542618-9542637 | Msa0938030:intron | 45.0% | |
| CCAGTTGTCCAATTGTGAAG+TGG | + | chr6_3:9542629-9542648 | None:intergenic | 45.0% | |
| CCACTTCACAATTGGACAAC+TGG | - | chr6_3:9542626-9542645 | Msa0938030:intron | 45.0% | |
| TAATGGCGAGTCACGTTCAA+TGG | + | chr6_3:9542671-9542690 | None:intergenic | 45.0% | |
| ! | GGCAAAGGATTTTGCTTTGG+TGG | - | chr6_3:9542704-9542723 | Msa0938030:intron | 45.0% |
| ! | CATGTACCGATCCTACTTGG+TGG | + | chr6_3:9542274-9542293 | None:intergenic | 50.0% |
| CACCAAGTAGGATCGGTACA+TGG | - | chr6_3:9542272-9542291 | Msa0938030:intron | 50.0% | |
| CACAATTGGACAACTGGAGC+TGG | - | chr6_3:9542632-9542651 | Msa0938030:intron | 50.0% | |
| ! | TTTTCCGGTGCAGCTTTTGG+TGG | + | chr6_3:9542767-9542786 | None:intergenic | 50.0% |
| !! | GCTTTTTCCGGTGCAGCTTT+TGG | + | chr6_3:9542770-9542789 | None:intergenic | 50.0% |
| ! | CATGTACCGATCCTACTTGG+TGG | + | chr6_3:9542274-9542293 | None:intergenic | 50.0% |
| CACCAAGTAGGATCGGTACA+TGG | - | chr6_3:9542272-9542291 | Msa0938030:intron | 50.0% | |
| CACAATTGGACAACTGGAGC+TGG | - | chr6_3:9542632-9542651 | Msa0938030:intron | 50.0% | |
| ! | TTTTCCGGTGCAGCTTTTGG+TGG | + | chr6_3:9542767-9542786 | None:intergenic | 50.0% |
| !! | GCTTTTTCCGGTGCAGCTTT+TGG | + | chr6_3:9542770-9542789 | None:intergenic | 50.0% |
| ACCACCACCAAAAGCTGCAC+CGG | - | chr6_3:9542760-9542779 | Msa0938030:intron | 55.0% | |
| ACCACCACCAAAAGCTGCAC+CGG | - | chr6_3:9542760-9542779 | Msa0938030:intron | 55.0% | |
| !!! | GGTGCAGCTTTTGGTGGTGG+TGG | + | chr6_3:9542761-9542780 | None:intergenic | 60.0% |
| ! | TCCGGTGCAGCTTTTGGTGG+TGG | + | chr6_3:9542764-9542783 | None:intergenic | 60.0% |
| !!! | GGTGCAGCTTTTGGTGGTGG+TGG | + | chr6_3:9542761-9542780 | None:intergenic | 60.0% |
| ! | TCCGGTGCAGCTTTTGGTGG+TGG | + | chr6_3:9542764-9542783 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 9541953 | 9542886 | 9541953 | ID=Msa0938030;Name=Msa0938030 |
| chr6_3 | mRNA | 9541953 | 9542886 | 9541953 | ID=Msa0938030-mRNA-1;Parent=Msa0938030;Name=Msa0938030-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|0.5|1|1|2|0|108 |
| chr6_3 | exon | 9542882 | 9542886 | 9542882 | ID=Msa0938030-mRNA-1:exon:3087;Parent=Msa0938030-mRNA-1 |
| chr6_3 | exon | 9541953 | 9542274 | 9541953 | ID=Msa0938030-mRNA-1:exon:3086;Parent=Msa0938030-mRNA-1 |
| chr6_3 | CDS | 9542882 | 9542886 | 9542882 | ID=Msa0938030-mRNA-1:cds;Parent=Msa0938030-mRNA-1 |
| chr6_3 | CDS | 9541953 | 9542274 | 9541953 | ID=Msa0938030-mRNA-1:cds;Parent=Msa0938030-mRNA-1 |
| Gene Sequence |
| Protein sequence |
