Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0200260 | Msa0958150 | 0.816662 | 4.926636e-52 | -8.615850e-47 |
| Msa0243100 | Msa0958150 | 0.822616 | 2.153551e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0958150 | MtrunA17_Chr4g0036071 | 82.857 | 35 | 6 | 0 | 44 | 78 | 376 | 410 | 5.86e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCTTGGTGATTATGTTAT+AGG | 0.277674 | 6_3:+71297123 | Msa0958150:CDS |
| TGCACAACTTACTTTGTTGC+AGG | 0.302616 | 6_3:+71297657 | Msa0958150:CDS |
| GAGCAAGATGCGATACATTT+CGG | 0.378242 | 6_3:+71297585 | Msa0958150:CDS |
| TTGGTGATTATGTTATAGGA+TGG | 0.539481 | 6_3:+71297127 | Msa0958150:CDS |
| GGGAATGCAACAATCAAGAA+AGG | 0.553987 | 6_3:+71297103 | Msa0958150:CDS |
| TGCAACAATCAAGAAAGGCT+TGG | 0.555852 | 6_3:+71297108 | Msa0958150:CDS |
| AGACTCACGCTGGCATGAAG+TGG | 0.570842 | 6_3:+71297035 | Msa0958150:CDS |
| TTTGTTGTAGAAGATGCAGA+AGG | 0.577392 | 6_3:+71297082 | Msa0958150:CDS |
| TTATAGGATGGAGAGGCCTA+AGG | 0.583526 | 6_3:+71297139 | Msa0958150:CDS |
| CATTTCGGAGCTAGAACACA+AGG | 0.586933 | 6_3:+71297600 | Msa0958150:CDS |
| AGATTTGTGATGGTTCGTGT+TGG | 0.587736 | 6_3:+71297012 | None:intergenic |
| TCTTCTACAACAAAAGTGCA+TGG | 0.635650 | 6_3:-71297073 | None:intergenic |
| GATTATGTTATAGGATGGAG+AGG | 0.635830 | 6_3:+71297132 | Msa0958150:CDS |
| TTGTTGTAGAAGATGCAGAA+GGG | 0.637293 | 6_3:+71297083 | Msa0958150:CDS |
| TTCGTGTTGGAGACTCACGC+TGG | 0.660671 | 6_3:+71297025 | Msa0958150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCAAAAATAAACTTATTC+AGG | + | chr6_3:71297307-71297326 | Msa0958150:intron | 15.0% |
| !!! | TTTTAGTCATTTTAGGATAA+AGG | - | chr6_3:71297207-71297226 | None:intergenic | 20.0% |
| !!! | CATGTATTTTTAGTCATTTT+AGG | - | chr6_3:71297214-71297233 | None:intergenic | 20.0% |
| !!! | TTTATTGACTATAGGTTTTA+GGG | - | chr6_3:71297348-71297367 | None:intergenic | 20.0% |
| !!! | CTTTATTGACTATAGGTTTT+AGG | - | chr6_3:71297349-71297368 | None:intergenic | 25.0% |
| ! | CTAAAACCTATAGTCAATAA+AGG | + | chr6_3:71297347-71297366 | Msa0958150:intron | 25.0% |
| ! | AGAAATGATCAATAGAGATT+TGG | - | chr6_3:71297480-71297499 | None:intergenic | 25.0% |
| ! | GTGTTAAATTATTATGACAG+CGG | + | chr6_3:71297536-71297555 | Msa0958150:intron | 25.0% |
| ! | TTGGTGATTATGTTATAGGA+TGG | + | chr6_3:71297127-71297146 | Msa0958150:CDS | 30.0% |
| !!! | TAGTCATTTTAGGATAAAGG+AGG | - | chr6_3:71297204-71297223 | None:intergenic | 30.0% |
| AAAGAAAATCTTCATCAGTC+AGG | + | chr6_3:71297399-71297418 | Msa0958150:intron | 30.0% | |
| GTTATATCCATGCTTATTAC+TGG | + | chr6_3:71297441-71297460 | Msa0958150:intron | 30.0% | |
| ! | TCTTCTACAACAAAAGTGCA+TGG | - | chr6_3:71297076-71297095 | None:intergenic | 35.0% |
| ! | TTTGTTGTAGAAGATGCAGA+AGG | + | chr6_3:71297082-71297101 | Msa0958150:CDS | 35.0% |
| TTGTTGTAGAAGATGCAGAA+GGG | + | chr6_3:71297083-71297102 | Msa0958150:CDS | 35.0% | |
| !! | AGGCTTGGTGATTATGTTAT+AGG | + | chr6_3:71297123-71297142 | Msa0958150:CDS | 35.0% |
| GATTATGTTATAGGATGGAG+AGG | + | chr6_3:71297132-71297151 | Msa0958150:CDS | 35.0% | |
| TTGTAACTTTCGCCAATTCA+AGG | + | chr6_3:71297235-71297254 | Msa0958150:intron | 35.0% | |
| ! | AGTGGACCTTTATTGACTAT+AGG | - | chr6_3:71297356-71297375 | None:intergenic | 35.0% |
| ATAGTCAATAAAGGTCCACT+TGG | + | chr6_3:71297356-71297375 | Msa0958150:intron | 35.0% | |
| TTTGAGTAATCTATGCCAAG+TGG | - | chr6_3:71297374-71297393 | None:intergenic | 35.0% | |
| CTTAGCTTCTTACTAATCAG+AGG | - | chr6_3:71297511-71297530 | None:intergenic | 35.0% | |
| CTAATGGCACAACAAAATCT+TGG | - | chr6_3:71297565-71297584 | None:intergenic | 35.0% | |
| !! | CAAGATTTTGTTGTGCCATT+AGG | + | chr6_3:71297563-71297582 | Msa0958150:intron | 35.0% |
| GGGAATGCAACAATCAAGAA+AGG | + | chr6_3:71297103-71297122 | Msa0958150:CDS | 40.0% | |
| TGCAACAATCAAGAAAGGCT+TGG | + | chr6_3:71297108-71297127 | Msa0958150:CDS | 40.0% | |
| AAGCAACCATTGTTCACCTT+AGG | - | chr6_3:71297158-71297177 | None:intergenic | 40.0% | |
| AAATGCAGTGAGCCTTGAAT+TGG | - | chr6_3:71297250-71297269 | None:intergenic | 40.0% | |
| TATCCATGCTTATTACTGGC+AGG | + | chr6_3:71297445-71297464 | Msa0958150:intron | 40.0% | |
| GAGCAAGATGCGATACATTT+CGG | + | chr6_3:71297585-71297604 | Msa0958150:CDS | 40.0% | |
| ! | AAGTTGTGCAAAAAGTGCAG+TGG | - | chr6_3:71297647-71297666 | None:intergenic | 40.0% |
| TGCACAACTTACTTTGTTGC+AGG | + | chr6_3:71297657-71297676 | Msa0958150:CDS | 40.0% | |
| TTATAGGATGGAGAGGCCTA+AGG | + | chr6_3:71297139-71297158 | Msa0958150:CDS | 45.0% | |
| GTATCGCATCTTGCTCCTAA+TGG | - | chr6_3:71297581-71297600 | None:intergenic | 45.0% | |
| CATTTCGGAGCTAGAACACA+AGG | + | chr6_3:71297600-71297619 | Msa0958150:CDS | 45.0% | |
| GAGAGGCCTAAGGTGAACAA+TGG | + | chr6_3:71297149-71297168 | Msa0958150:intron | 50.0% | |
| CTGCCTGCCAGTAATAAGCA+TGG | - | chr6_3:71297451-71297470 | None:intergenic | 50.0% | |
| TTCGTGTTGGAGACTCACGC+TGG | + | chr6_3:71297025-71297044 | Msa0958150:CDS | 55.0% | |
| AGACTCACGCTGGCATGAAG+TGG | + | chr6_3:71297035-71297054 | Msa0958150:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 71297021 | 71297684 | 71297021 | ID=Msa0958150;Name=Msa0958150 |
| chr6_3 | mRNA | 71297021 | 71297684 | 71297021 | ID=Msa0958150-mRNA-1;Parent=Msa0958150;Name=Msa0958150-mRNA-1;_AED=0.42;_eAED=0.74;_QI=0|0|0|1|1|1|2|0|79 |
| chr6_3 | exon | 71297021 | 71297160 | 71297021 | ID=Msa0958150-mRNA-1:exon:12990;Parent=Msa0958150-mRNA-1 |
| chr6_3 | exon | 71297585 | 71297684 | 71297585 | ID=Msa0958150-mRNA-1:exon:12991;Parent=Msa0958150-mRNA-1 |
| chr6_3 | CDS | 71297021 | 71297160 | 71297021 | ID=Msa0958150-mRNA-1:cds;Parent=Msa0958150-mRNA-1 |
| chr6_3 | CDS | 71297585 | 71297684 | 71297585 | ID=Msa0958150-mRNA-1:cds;Parent=Msa0958150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
