Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0960690 | KEH26731.1 | 78.571 | 56 | 8 | 1 | 4 | 55 | 50 | 105 | 7.67e-19 | 84.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0960690 | A0A072UBA3 | 78.571 | 56 | 8 | 1 | 4 | 55 | 50 | 105 | 3.67e-19 | 84.0 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0960690 | Msa0973600 | 0.809341 | 1.984578e-50 | -8.615850e-47 |
| Msa0960690 | Msa1163540 | 0.805135 | 1.544802e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0960690 | MtrunA17_Chr6g0479011 | 78.571 | 56 | 8 | 1 | 4 | 55 | 50 | 105 | 7.06e-23 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 3 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAAATTCAAGCAATATC+TGG | 0.402067 | 6_3:-80584132 | Msa0960690:CDS |
| TTACGAGGAATACTTGTAAG+AGG | 0.513515 | 6_3:+80585009 | None:intergenic |
| ATTACTAATTACTGATGCAA+AGG | 0.561735 | 6_3:-80585146 | Msa0960690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGAAATTTGAAACTATTAA+TGG | - | chr6_3:80584686-80584705 | Msa0960690:intron | 15.0% |
| !! | AAATATTACACTATGAATTA+GGG | - | chr6_3:80584885-80584904 | Msa0960690:intron | 15.0% |
| !!! | TTGAAATTTGAAACTATTAA+TGG | - | chr6_3:80584686-80584705 | Msa0960690:intron | 15.0% |
| !! | AAATATTACACTATGAATTA+GGG | - | chr6_3:80584885-80584904 | Msa0960690:intron | 15.0% |
| !!! | TAATTAGTAATCTTTTTGGT+GGG | + | chr6_3:80584115-80584134 | None:intergenic | 20.0% |
| !!! | TCAGTAATTAGTAATCTTTT+TGG | + | chr6_3:80584119-80584138 | None:intergenic | 20.0% |
| !! | TATTGAAAAATTTCATCTGA+TGG | - | chr6_3:80584597-80584616 | Msa0960690:intron | 20.0% |
| !! | GAAATATTACACTATGAATT+AGG | - | chr6_3:80584884-80584903 | Msa0960690:intron | 20.0% |
| !! | AATATTACACTATGAATTAG+GGG | - | chr6_3:80584886-80584905 | Msa0960690:intron | 20.0% |
| !! | TATGTAAATATGTACTGAAA+AGG | + | chr6_3:80585009-80585028 | None:intergenic | 20.0% |
| !!! | TTAGAAGTACATGTAAATTA+TGG | + | chr6_3:80585064-80585083 | None:intergenic | 20.0% |
| !!! | ATTTACATGTACTTCTAAAA+TGG | - | chr6_3:80585066-80585085 | Msa0960690:CDS | 20.0% |
| !!! | TAATTAGTAATCTTTTTGGT+GGG | + | chr6_3:80584115-80584134 | None:intergenic | 20.0% |
| !!! | TCAGTAATTAGTAATCTTTT+TGG | + | chr6_3:80584119-80584138 | None:intergenic | 20.0% |
| !! | TATTGAAAAATTTCATCTGA+TGG | - | chr6_3:80584597-80584616 | Msa0960690:intron | 20.0% |
| !! | GAAATATTACACTATGAATT+AGG | - | chr6_3:80584884-80584903 | Msa0960690:intron | 20.0% |
| !! | AATATTACACTATGAATTAG+GGG | - | chr6_3:80584886-80584905 | Msa0960690:intron | 20.0% |
| !! | TATGTAAATATGTACTGAAA+AGG | + | chr6_3:80585009-80585028 | None:intergenic | 20.0% |
| !!! | TTAGAAGTACATGTAAATTA+TGG | + | chr6_3:80585064-80585083 | None:intergenic | 20.0% |
| !!! | ATTTACATGTACTTCTAAAA+TGG | - | chr6_3:80585066-80585085 | Msa0960690:CDS | 20.0% |
| !! | GTAATTAGTAATCTTTTTGG+TGG | + | chr6_3:80584116-80584135 | None:intergenic | 25.0% |
| ! | ATTACTAATTACTGATGCAA+AGG | - | chr6_3:80584124-80584143 | Msa0960690:CDS | 25.0% |
| !! | GTGGATTTTATCTTGAATTT+TGG | + | chr6_3:80584196-80584215 | None:intergenic | 25.0% |
| !!! | TGATTTTGTAGTTTTAGATG+TGG | + | chr6_3:80584215-80584234 | None:intergenic | 25.0% |
| ! | ATTTGCAACAAATTAACAAG+TGG | - | chr6_3:80584506-80584525 | Msa0960690:intron | 25.0% |
| ! | AAATTAACAAGTGGATATGT+TGG | - | chr6_3:80584515-80584534 | Msa0960690:intron | 25.0% |
| !!! | AGTTATTTCTGTTATTGACT+CGG | + | chr6_3:80584714-80584733 | None:intergenic | 25.0% |
| !!! | ATCAATATTTGTTTTCCAGT+AGG | + | chr6_3:80584826-80584845 | None:intergenic | 25.0% |
| !! | GTAATTAGTAATCTTTTTGG+TGG | + | chr6_3:80584116-80584135 | None:intergenic | 25.0% |
| ! | ATTACTAATTACTGATGCAA+AGG | - | chr6_3:80584124-80584143 | Msa0960690:CDS | 25.0% |
| !! | GTGGATTTTATCTTGAATTT+TGG | + | chr6_3:80584196-80584215 | None:intergenic | 25.0% |
| !!! | TGATTTTGTAGTTTTAGATG+TGG | + | chr6_3:80584215-80584234 | None:intergenic | 25.0% |
| ! | ATTTGCAACAAATTAACAAG+TGG | - | chr6_3:80584506-80584525 | Msa0960690:intron | 25.0% |
| ! | AAATTAACAAGTGGATATGT+TGG | - | chr6_3:80584515-80584534 | Msa0960690:intron | 25.0% |
| !!! | AGTTATTTCTGTTATTGACT+CGG | + | chr6_3:80584714-80584733 | None:intergenic | 25.0% |
| !!! | ATCAATATTTGTTTTCCAGT+AGG | + | chr6_3:80584826-80584845 | None:intergenic | 25.0% |
| TTGTTGCAAATAGCTTAAAG+TGG | + | chr6_3:80584497-80584516 | None:intergenic | 30.0% | |
| ! | AAAAGAATGCTAGTTTACTC+AGG | - | chr6_3:80584556-80584575 | Msa0960690:intron | 30.0% |
| GAAAAATTTCATCTGATGGA+AGG | - | chr6_3:80584601-80584620 | Msa0960690:intron | 30.0% | |
| TCTATTCTGTATAATGCAGA+TGG | - | chr6_3:80584640-80584659 | Msa0960690:intron | 30.0% | |
| ! | ACAGATCTAAAATATAAGCC+AGG | + | chr6_3:80584667-80584686 | None:intergenic | 30.0% |
| GAAATTACAGAAACTACATG+AGG | + | chr6_3:80584857-80584876 | None:intergenic | 30.0% | |
| ACATTCAAATACATTATCCG+AGG | - | chr6_3:80585031-80585050 | Msa0960690:CDS | 30.0% | |
| GAAGTACATGTAAATTATGG+AGG | + | chr6_3:80585061-80585080 | None:intergenic | 30.0% | |
| CATCAAATTCAAGCAATATC+TGG | - | chr6_3:80585138-80585157 | Msa0960690:CDS | 30.0% | |
| TTGTTGCAAATAGCTTAAAG+TGG | + | chr6_3:80584497-80584516 | None:intergenic | 30.0% | |
| ! | AAAAGAATGCTAGTTTACTC+AGG | - | chr6_3:80584556-80584575 | Msa0960690:intron | 30.0% |
| GAAAAATTTCATCTGATGGA+AGG | - | chr6_3:80584601-80584620 | Msa0960690:intron | 30.0% | |
| TCTATTCTGTATAATGCAGA+TGG | - | chr6_3:80584640-80584659 | Msa0960690:intron | 30.0% | |
| ! | ACAGATCTAAAATATAAGCC+AGG | + | chr6_3:80584667-80584686 | None:intergenic | 30.0% |
| GAAATTACAGAAACTACATG+AGG | + | chr6_3:80584857-80584876 | None:intergenic | 30.0% | |
| ACATTCAAATACATTATCCG+AGG | - | chr6_3:80585031-80585050 | Msa0960690:CDS | 30.0% | |
| GAAGTACATGTAAATTATGG+AGG | + | chr6_3:80585061-80585080 | None:intergenic | 30.0% | |
| CATCAAATTCAAGCAATATC+TGG | - | chr6_3:80585138-80585157 | Msa0960690:CDS | 30.0% | |
| TTACGAGGAATACTTGTAAG+AGG | + | chr6_3:80584264-80584283 | None:intergenic | 35.0% | |
| TTATTGTGTGTGTACAACTC+AGG | - | chr6_3:80584358-80584377 | Msa0960690:intron | 35.0% | |
| GTACTGAAAAGGCAAATATC+AGG | + | chr6_3:80584998-80585017 | None:intergenic | 35.0% | |
| TCAAATACATTATCCGAGGT+TGG | - | chr6_3:80585035-80585054 | Msa0960690:CDS | 35.0% | |
| TTACGAGGAATACTTGTAAG+AGG | + | chr6_3:80584264-80584283 | None:intergenic | 35.0% | |
| TTATTGTGTGTGTACAACTC+AGG | - | chr6_3:80584358-80584377 | Msa0960690:intron | 35.0% | |
| GTACTGAAAAGGCAAATATC+AGG | + | chr6_3:80584998-80585017 | None:intergenic | 35.0% | |
| TCAAATACATTATCCGAGGT+TGG | - | chr6_3:80585035-80585054 | Msa0960690:CDS | 35.0% | |
| TACGAGGAACATAACTTACG+AGG | + | chr6_3:80584279-80584298 | None:intergenic | 40.0% | |
| TGTGTAACGAGTAACTAAGC+AGG | + | chr6_3:80584314-80584333 | None:intergenic | 40.0% | |
| CTCAGGACATACATCATATG+TGG | - | chr6_3:80584573-80584592 | Msa0960690:intron | 40.0% | |
| ACTTGAAGAGAGAGGATTCA+AGG | + | chr6_3:80584740-80584759 | None:intergenic | 40.0% | |
| !! | ATCCAGACTACTTTTCCTAC+TGG | - | chr6_3:80584808-80584827 | Msa0960690:intron | 40.0% |
| TTCCAGTAGGAAAAGTAGTC+TGG | + | chr6_3:80584813-80584832 | None:intergenic | 40.0% | |
| TAAATTATGGAGGCCAACCT+CGG | + | chr6_3:80585051-80585070 | None:intergenic | 40.0% | |
| TTTCGTTCCTGTACGTTTAG+AGG | + | chr6_3:80585117-80585136 | None:intergenic | 40.0% | |
| TACGAGGAACATAACTTACG+AGG | + | chr6_3:80584279-80584298 | None:intergenic | 40.0% | |
| TGTGTAACGAGTAACTAAGC+AGG | + | chr6_3:80584314-80584333 | None:intergenic | 40.0% | |
| CTCAGGACATACATCATATG+TGG | - | chr6_3:80584573-80584592 | Msa0960690:intron | 40.0% | |
| ACTTGAAGAGAGAGGATTCA+AGG | + | chr6_3:80584740-80584759 | None:intergenic | 40.0% | |
| !! | ATCCAGACTACTTTTCCTAC+TGG | - | chr6_3:80584808-80584827 | Msa0960690:intron | 40.0% |
| TTCCAGTAGGAAAAGTAGTC+TGG | + | chr6_3:80584813-80584832 | None:intergenic | 40.0% | |
| TAAATTATGGAGGCCAACCT+CGG | + | chr6_3:80585051-80585070 | None:intergenic | 40.0% | |
| TTTCGTTCCTGTACGTTTAG+AGG | + | chr6_3:80585117-80585136 | None:intergenic | 40.0% | |
| CAGGTCATGAAGTTGTTACG+AGG | + | chr6_3:80584295-80584314 | None:intergenic | 45.0% | |
| !! | GCAGCAGCAAGAGTATTCTA+AGG | - | chr6_3:80584419-80584438 | Msa0960690:intron | 45.0% |
| CTGTATAATGCAGATGGTCC+TGG | - | chr6_3:80584646-80584665 | Msa0960690:intron | 45.0% | |
| ATGCATGCCTCTAAACGTAC+AGG | - | chr6_3:80585107-80585126 | Msa0960690:intron | 45.0% | |
| CAGGTCATGAAGTTGTTACG+AGG | + | chr6_3:80584295-80584314 | None:intergenic | 45.0% | |
| !! | GCAGCAGCAAGAGTATTCTA+AGG | - | chr6_3:80584419-80584438 | Msa0960690:intron | 45.0% |
| CTGTATAATGCAGATGGTCC+TGG | - | chr6_3:80584646-80584665 | Msa0960690:intron | 45.0% | |
| ATGCATGCCTCTAAACGTAC+AGG | - | chr6_3:80585107-80585126 | Msa0960690:intron | 45.0% | |
| GTGGCATGACTTGAAGAGAG+AGG | + | chr6_3:80584748-80584767 | None:intergenic | 50.0% | |
| ATCACTAGCAGAGTGCAGTG+TGG | + | chr6_3:80584767-80584786 | None:intergenic | 50.0% | |
| GTGGCATGACTTGAAGAGAG+AGG | + | chr6_3:80584748-80584767 | None:intergenic | 50.0% | |
| ATCACTAGCAGAGTGCAGTG+TGG | + | chr6_3:80584767-80584786 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 80584095 | 80585197 | 80584095 | ID=Msa0960690;Name=Msa0960690 |
| chr6_3 | mRNA | 80584095 | 80585197 | 80584095 | ID=Msa0960690-mRNA-1;Parent=Msa0960690;Name=Msa0960690-mRNA-1;_AED=0.53;_eAED=0.53;_QI=0|0|0|0.66|0|0|3|0|78 |
| chr6_3 | exon | 80585135 | 80585197 | 80585135 | ID=Msa0960690-mRNA-1:exon:14471;Parent=Msa0960690-mRNA-1 |
| chr6_3 | exon | 80585013 | 80585117 | 80585013 | ID=Msa0960690-mRNA-1:exon:14470;Parent=Msa0960690-mRNA-1 |
| chr6_3 | exon | 80584095 | 80584163 | 80584095 | ID=Msa0960690-mRNA-1:exon:14469;Parent=Msa0960690-mRNA-1 |
| chr6_3 | CDS | 80585135 | 80585197 | 80585135 | ID=Msa0960690-mRNA-1:cds;Parent=Msa0960690-mRNA-1 |
| chr6_3 | CDS | 80585013 | 80585117 | 80585013 | ID=Msa0960690-mRNA-1:cds;Parent=Msa0960690-mRNA-1 |
| chr6_3 | CDS | 80584095 | 80584163 | 80584095 | ID=Msa0960690-mRNA-1:cds;Parent=Msa0960690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
