Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0961110 | AES98942.1 | 87.500 | 48 | 6 | 0 | 15 | 62 | 105 | 152 | 1.23e-19 | 89.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0961110 | G7KFL1 | 87.500 | 48 | 6 | 0 | 15 | 62 | 105 | 152 | 5.89e-20 | 89.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0828560 | Msa0961110 | 0.806680 | 7.315018e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTAATTATTTGTTTCCT+AGG | 0.318105 | 6_3:+81890270 | None:intergenic |
| ATATTCCTTGCATCATGGTT+GGG | 0.382746 | 6_3:-81890497 | Msa0961110:CDS |
| TATATTCCTTGCATCATGGT+TGG | 0.414367 | 6_3:-81890498 | Msa0961110:CDS |
| ATGCAGGGATATGCTATTGT+CGG | 0.496724 | 6_3:-81890549 | Msa0961110:CDS |
| GTTGTATATTCCTTGCATCA+TGG | 0.504508 | 6_3:-81890502 | Msa0961110:CDS |
| GCATCAAAATCGAACACAAA+GGG | 0.538533 | 6_3:+81890568 | None:intergenic |
| GTTTCCTAGGAAATATTCCT+CGG | 0.538581 | 6_3:+81890283 | None:intergenic |
| GGCTGTTGTTGATCCTGCAG+TGG | 0.590761 | 6_3:-81890620 | Msa0961110:CDS |
| CAAGACCCAACCATGATGCA+AGG | 0.629871 | 6_3:+81890492 | None:intergenic |
| TGCATCAAAATCGAACACAA+AGG | 0.645880 | 6_3:+81890567 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAATTAACATAATTTTAG+GGG | - | chr6_3:81890684-81890703 | Msa0961110:intron | 10.0% |
| !!! | GAATATATTTTCCGTTTATT+TGG | - | chr6_3:81890626-81890645 | Msa0961110:CDS | 20.0% |
| !! | ATTTCCAATATCCAAATAAA+CGG | + | chr6_3:81890640-81890659 | None:intergenic | 20.0% |
| !!! | ATGATGCTTTAGATAGTTTT+AGG | - | chr6_3:81890326-81890345 | Msa0961110:intron | 25.0% |
| !!! | GAGAGTTTTAAAGTACAAAA+GGG | + | chr6_3:81890600-81890619 | None:intergenic | 25.0% |
| !! | TTTTCCGTTTATTTGGATAT+TGG | - | chr6_3:81890633-81890652 | Msa0961110:CDS | 25.0% |
| !!! | TATTTTTTAGTAGTTTTCCG+AGG | - | chr6_3:81890732-81890751 | Msa0961110:intron | 25.0% |
| !!! | ATAGTTTTAGGCTAGTTTTG+TGG | - | chr6_3:81890338-81890357 | Msa0961110:intron | 30.0% |
| !! | GCATTTATGCTCTTTTGTTT+TGG | - | chr6_3:81890391-81890410 | Msa0961110:intron | 30.0% |
| !!! | GCAGTTTTTAGGTTCTATTT+TGG | - | chr6_3:81890434-81890453 | Msa0961110:intron | 30.0% |
| !!! | TTTTTAGGTTCTATTTTGGC+TGG | - | chr6_3:81890438-81890457 | Msa0961110:intron | 30.0% |
| !!! | CGAGAGTTTTAAAGTACAAA+AGG | + | chr6_3:81890601-81890620 | None:intergenic | 30.0% |
| ! | GCATTTATGCTCTTCTGTTT+TGG | - | chr6_3:81890298-81890317 | Msa0961110:intron | 35.0% |
| GCATCAAAATCGAACACAAA+GGG | + | chr6_3:81890467-81890486 | None:intergenic | 35.0% | |
| TGCATCAAAATCGAACACAA+AGG | + | chr6_3:81890468-81890487 | None:intergenic | 35.0% | |
| !! | TTTGTGTTCGATTTTGATGC+AGG | - | chr6_3:81890467-81890486 | Msa0961110:intron | 35.0% |
| !! | TTGTGTTCGATTTTGATGCA+GGG | - | chr6_3:81890468-81890487 | Msa0961110:intron | 35.0% |
| GTTGTATATTCCTTGCATCA+TGG | - | chr6_3:81890530-81890549 | Msa0961110:CDS | 35.0% | |
| TATATTCCTTGCATCATGGT+TGG | - | chr6_3:81890534-81890553 | Msa0961110:CDS | 35.0% | |
| ATATTCCTTGCATCATGGTT+GGG | - | chr6_3:81890535-81890554 | Msa0961110:CDS | 35.0% | |
| ! | TTTTCCGAGGAATATTTCCT+AGG | - | chr6_3:81890745-81890764 | Msa0961110:intron | 35.0% |
| GTTTCCTAGGAAATATTCCT+CGG | + | chr6_3:81890752-81890771 | None:intergenic | 35.0% | |
| !!! | TTAGGTTCTATTTTGGCTGG+TGG | - | chr6_3:81890441-81890460 | Msa0961110:intron | 40.0% |
| ATGCAGGGATATGCTATTGT+CGG | - | chr6_3:81890483-81890502 | Msa0961110:intron | 40.0% | |
| !!! | TCATGGTTGGGTCTTGTTTT+TGG | - | chr6_3:81890547-81890566 | Msa0961110:CDS | 40.0% |
| !!! | CATGGTTGGGTCTTGTTTTT+GGG | - | chr6_3:81890548-81890567 | Msa0961110:CDS | 40.0% |
| !!! | GGGTCTTGTTTTTGGGTTTT+AGG | - | chr6_3:81890555-81890574 | Msa0961110:CDS | 40.0% |
| TTAGGTGTGCCGCATATATA+CGG | - | chr6_3:81890573-81890592 | Msa0961110:CDS | 40.0% | |
| CAAAAGGGACCGTATATATG+CGG | + | chr6_3:81890585-81890604 | None:intergenic | 40.0% | |
| ! | ATCCTGCAGTGGCAGTTTTT+AGG | - | chr6_3:81890423-81890442 | Msa0961110:intron | 45.0% |
| AACCTAAAAACTGCCACTGC+AGG | + | chr6_3:81890428-81890447 | None:intergenic | 45.0% | |
| !! | ATTGTCGGCATCGTTTTTGC+TGG | - | chr6_3:81890498-81890517 | Msa0961110:CDS | 45.0% |
| !!! | TATTTAATTAACATAATTTT+AGG | - | chr6_3:81890682-81890701 | Msa0961110:intron | 5.0% |
| !!! | ATTTAATTAACATAATTTTA+GGG | - | chr6_3:81890683-81890702 | Msa0961110:intron | 5.0% |
| !! | GGCATCGTTTTTGCTGGTTG+TGG | - | chr6_3:81890504-81890523 | Msa0961110:CDS | 50.0% |
| CAAGACCCAACCATGATGCA+AGG | + | chr6_3:81890543-81890562 | None:intergenic | 50.0% | |
| GGCTGTTGTTGATCCTGCAG+TGG | - | chr6_3:81890412-81890431 | Msa0961110:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 81890277 | 81890777 | 81890277 | ID=Msa0961110;Name=Msa0961110 |
| chr6_3 | mRNA | 81890277 | 81890777 | 81890277 | ID=Msa0961110-mRNA-1;Parent=Msa0961110;Name=Msa0961110-mRNA-1;_AED=0.54;_eAED=0.64;_QI=0|0|0|0.66|0|0|3|0|72 |
| chr6_3 | exon | 81890770 | 81890777 | 81890770 | ID=Msa0961110-mRNA-1:exon:14687;Parent=Msa0961110-mRNA-1 |
| chr6_3 | exon | 81890485 | 81890662 | 81890485 | ID=Msa0961110-mRNA-1:exon:14686;Parent=Msa0961110-mRNA-1 |
| chr6_3 | exon | 81890277 | 81890309 | 81890277 | ID=Msa0961110-mRNA-1:exon:14685;Parent=Msa0961110-mRNA-1 |
| chr6_3 | CDS | 81890770 | 81890777 | 81890770 | ID=Msa0961110-mRNA-1:cds;Parent=Msa0961110-mRNA-1 |
| chr6_3 | CDS | 81890485 | 81890662 | 81890485 | ID=Msa0961110-mRNA-1:cds;Parent=Msa0961110-mRNA-1 |
| chr6_3 | CDS | 81890277 | 81890309 | 81890277 | ID=Msa0961110-mRNA-1:cds;Parent=Msa0961110-mRNA-1 |
| Gene Sequence |
| Protein sequence |
