AlfalfaGEDB | Msa0961830

M. sativa cultivar zhongmu-4 / Msa0961830

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009825 GO:0009835 GO:0009889 GO:0009908 GO:0009987 GO:0010150 GO:0010154 GO:0010468 GO:0010556 GO:0016049 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071695 GO:0080090 GO:0090567 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961830	sp\|Q9FY93\|NAC83_ARATH	73.653	167	42	1	1	165	1	167	7.05e-91	270

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961830	A0A896WEL3	84.348	230	29	2	1	223	1	230	2.23e-134	387

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TFs/TRs:

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Gene ID	Type	Classification
Msa0961830	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0893610	Msa0961830	0.956386	3.330827e-114	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961830	MtrunA17_Chr7g0214071	79.688	256	21	6	1	225	1	256	1.61e-133	375
Msa0961830	MtrunA17_Chr8g0385991	60.795	176	58	3	1	166	1	175	5.85e-74	223
Msa0961830	MtrunA17_Chr3g0131911	58.427	178	62	3	1	166	1	178	1.12e-69	213
Msa0961830	MtrunA17_Chr4g0047841	54.857	175	50	3	13	158	14	188	1.27e-57	186
Msa0961830	MtrunA17_Chr7g0252971	45.946	222	91	7	11	203	5	226	4.33e-57	184
Msa0961830	MtrunA17_Chr5g0417791	56.962	158	56	3	12	159	1	156	1.99e-56	180
Msa0961830	MtrunA17_Chr4g0042561	53.977	176	69	4	14	179	9	182	2.11e-56	180
Msa0961830	MtrunA17_Chr2g0318101	56.173	162	54	2	14	158	15	176	3.56e-56	182
Msa0961830	MtrunA17_Chr7g0263931	47.368	190	89	4	9	188	6	194	9.33e-55	177
Msa0961830	MtrunA17_Chr7g0243371	89.773	88	9	0	59	146	12	99	3.94e-54	169

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961830	AT5G13180.1	73.653	167	42	1	1	165	1	167	7.17e-92	270
Msa0961830	AT2G33480.2	60.000	205	78	3	1	203	1	203	2.07e-84	252
Msa0961830	AT2G33480.1	59.420	207	78	3	1	203	1	205	4.53e-84	251
Msa0961830	AT1G61110.1	56.886	167	53	4	10	158	12	177	3.55e-56	181
Msa0961830	AT1G77450.1	49.746	197	85	4	10	196	6	198	5.21e-56	179
Msa0961830	AT3G04070.2	55.689	167	58	4	8	158	4	170	1.15e-55	181
Msa0961830	AT1G69490.1	57.143	161	56	4	14	164	9	166	1.46e-55	178
Msa0961830	AT5G39610.1	45.714	210	99	5	12	208	18	225	1.82e-54	176
Msa0961830	AT3G04070.1	52.542	177	52	4	14	158	10	186	1.41e-53	176
Msa0961830	AT1G65910.1	45.411	207	95	5	12	210	4	200	2.36e-53	181

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Find 51 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGATGGGATTGAGG+AGG	0.660083	6_3:-84272458	None:intergenic
AAAGAAGCCAAGTATCCAAA+TGG	0.440359	6_3:+84271931	Msa0961830:CDS
AAGAAGCCAAGTATCCAAAT+GGG	0.544681	6_3:+84271932	Msa0961830:CDS
AAGATCAACTTCAGGAATGA+TGG	0.468508	6_3:-84271659	None:intergenic
AAGCATTTCCAAAAGAGGAT+GGG	0.533996	6_3:-84272468	None:intergenic
AATGGAGAGGAGACAGTGAA+AGG	0.524667	6_3:+84272307	Msa0961830:CDS
ACAACAAGTTCTTCATCTGT+TGG	0.464543	6_3:-84271592	None:intergenic
ACTTCAGGAATGATGGAAGC+AGG	0.542259	6_3:-84271652	None:intergenic
AGAAAGACAGACCAATGGGT+TGG	0.571037	6_3:-84272142	None:intergenic
AGAGGATGGGATTGAGGAGG+AGG	0.387672	6_3:-84272455	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGTAGAAGTTATCATGTTC+CGG	-	chr6_3:84271824-84271843	None:intergenic	30.0%
TAGTAGAAGTTATCATGTTC+CGG	-	chr6_3:84271824-84271843	None:intergenic	30.0%
CTTCATCTGTTGGATGAAAA+CGG	-	chr6_3:84271585-84271604	None:intergenic	35.0%
ACAACAAGTTCTTCATCTGT+TGG	-	chr6_3:84271595-84271614	None:intergenic	35.0%
TGTTCAATACTTGAAGCGTA+AGG	+	chr6_3:84271612-84271631	Msa0961830:CDS	35.0%
AAGATCAACTTCAGGAATGA+TGG	-	chr6_3:84271662-84271681	None:intergenic	35.0%
AAAGAAGCCAAGTATCCAAA+TGG	+	chr6_3:84271931-84271950	Msa0961830:CDS	35.0%
AAGAAGCCAAGTATCCAAAT+GGG	+	chr6_3:84271932-84271951	Msa0961830:CDS	35.0%
AGTTTGTAGCTCTCTTAGAT+CGG	-	chr6_3:84271960-84271979	None:intergenic	35.0%
TCAAAGACTCATGAAGTTGT+TGG	+	chr6_3:84272024-84272043	Msa0961830:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	84271529	84272492	84271529	ID=Msa0961830;Name=Msa0961830
chr6_3	mRNA	84271529	84272492	84271529	ID=Msa0961830-mRNA-1;Parent=Msa0961830;Name=Msa0961830-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|3\|0\|225
chr6_3	exon	84271529	84271709	84271529	ID=Msa0961830-mRNA-1:exon:15124;Parent=Msa0961830-mRNA-1
chr6_3	exon	84271896	84272152	84271896	ID=Msa0961830-mRNA-1:exon:15125;Parent=Msa0961830-mRNA-1
chr6_3	exon	84272253	84272492	84272253	ID=Msa0961830-mRNA-1:exon:15126;Parent=Msa0961830-mRNA-1
chr6_3	CDS	84271529	84271709	84271529	ID=Msa0961830-mRNA-1:cds;Parent=Msa0961830-mRNA-1
chr6_3	CDS	84271896	84272152	84271896	ID=Msa0961830-mRNA-1:cds;Parent=Msa0961830-mRNA-1
chr6_3	CDS	84272253	84272492	84272253	ID=Msa0961830-mRNA-1:cds;Parent=Msa0961830-mRNA-1

Gene Sequence

>Msa0961830

ATGGACAAGATGAATTTTGTGAAGAATGGTGAAGTGAGATTGCCACCAGGTTTCCGTTTTCATCCAACAGATGAAGAACTTGTTGTTCAATACTTGAAGCGTAAGGTCTTCTCTTTCCCTTTACCTGCTTCCATCATTCCTGAAGTTGATCTTTGCAAGTCTGATCCTTGGGATTTACCTGGTGATATGGAACAAGAGAGGTACTTTTTCAGTACAAAAGAAGCCAAGTATCCAAATGGGAACCGATCTAAGAGAGCTACAAACTCTGGTTACTGGAAAGCAACTGGTTTGGGCAAACAAATCATGAATTCAAAGACTCATGAAGTTGTTGGCATGAAGAAAACTCTTGTTTTCTACAGAGGCAAACCACCTCATGGTTCCAGAACTGATTGGATCATGCATGAATATCGCCTCACTTCCTCTCACTCCAACCCATTGAATGAAAATTGGGTTCTGTGTCGCATATTTCTGAAGAGGAGAGGTGGTGCTAAAAATGGAGAGGAGACAGTGAAAGGAGTGAAGCCAAGCAATGACAGCAGTTTTGGTGAGGTGAAGAAGAACTCAGCTTCAACTTCAAGTTCAAAAGTGGTTGTTTTCTATGATTTCTTGGCAGAGAAGAAGAAAAATTCTGATGCATCCTCCTCCTCCTCAATCCCATCCTCTTTTGGAAATGCTTAA

Protein sequence

>Msa0961830

MDKMNFVKNGEVRLPPGFRFHPTDEELVVQYLKRKVFSFPLPASIIPEVDLCKSDPWDLPGDMEQERYFFSTKEAKYPNGNRSKRATNSGYWKATGLGKQIMNSKTHEVVGMKKTLVFYRGKPPHGSRTDWIMHEYRLTSSHSNPLNENWVLCRIFLKRRGGAKNGEETVKGVKPSNDSSFGEVKKNSASTSSSKVVVFYDFLAEKKKNSDASSSSSIPSSFGNA*