AlfalfaGEDB | Msa0968310

M. sativa cultivar zhongmu-4 / Msa0968310

KEGG: -
GO: -
PFAM: PF04689.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968310	sp\|Q7XLX6\|S1FA2_ORYSJ	80.328	61	12	0	24	84	20	80	1.18e-14	65.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968310	G7L1S4	92.857	84	6	0	1	84	1	84	5.37e-26	100

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TFs/TRs:

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Gene ID	Type	Classification
Msa0968310	TF	S1Fa-like

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0968310	Msa1169270	0.844031	9.721102e-59	-8.615850e-47
Msa0968310	Msa1170030	0.995806	4.270113e-220	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968310	MtrunA17_Chr7g0263831	92.857	84	6	0	1	84	1	84	1.03e-29	100
Msa0968310	MtrunA17_Chr7g0263871	89.474	76	3	1	9	84	3	73	1.59e-16	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968310	AT2G37120.1	78.689	61	13	0	24	84	16	76	6.03e-15	63.5
Msa0968310	AT3G53370.1	80.000	60	12	0	25	84	17	76	7.50e-13	58.2

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Find 35 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCAAGGAGTCTCTGCACC+TGG	0.523372	6_3:+98642567	Msa0968310:CDS
AATCTATTTAAAACAAACCG+TGG	0.654277	6_3:+98641960	Msa0968310:five_prime_UTR
ACAAACCGTGGTGCAAACTT+TGG	0.386650	6_3:+98641972	Msa0968310:five_prime_UTR
ACAGCAACCCACCAATAACC+AGG	0.598951	6_3:-98642437	None:intergenic
ACCAGGAGAACTATTAAGCC+TGG	0.450176	6_3:-98642420	None:intergenic
ACCATGCGATCAAAGGAAGG+AGG	0.683503	6_3:-98642075	None:intergenic
ACTTTCTGTCAATAACACAC+AGG	0.703987	6_3:-98642690	None:intergenic
AGAGTGCATAATTTCCTATG+AGG	0.578802	6_3:-98642467	None:intergenic
AGTACCATGCGATCAAAGGA+AGG	0.619049	6_3:-98642078	None:intergenic
AGTCTCTGCACCTGGAGAGT+AGG	0.526480	6_3:+98642575	Msa0968310:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTTTAGATTAGACATGTCT+TGG	+	chr6_3:98642175-98642194	Msa0968310:intron	30.0%
CAAGAGCAAAAACTTGATTA+AGG	-	chr6_3:98642363-98642382	None:intergenic	30.0%
TTAATAGTTCTCCTGGTTAT+TGG	+	chr6_3:98642426-98642445	Msa0968310:CDS	30.0%
TCTTTAGATTAGACATGTCT+TGG	+	chr6_3:98642175-98642194	Msa0968310:intron	30.0%
CAAGAGCAAAAACTTGATTA+AGG	-	chr6_3:98642363-98642382	None:intergenic	30.0%
TTAATAGTTCTCCTGGTTAT+TGG	+	chr6_3:98642426-98642445	Msa0968310:CDS	30.0%
ATCACCGAATTCAAAGTCAT+CGG	-	chr6_3:98642049-98642068	None:intergenic	35.0%
TTTAGAAAGCTGGATCTCTA+GGG	+	chr6_3:98642391-98642410	Msa0968310:intron	35.0%
AGAGTGCATAATTTCCTATG+AGG	-	chr6_3:98642470-98642489	None:intergenic	35.0%
CTCAAATGTTCTATGGAATG+TGG	+	chr6_3:98642607-98642626	Msa0968310:three_prime_UTR	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	98641951	98642828	98641951	ID=Msa0968310;Name=Msa0968310
chr6_3	mRNA	98641951	98642828	98641951	ID=Msa0968310-mRNA-1;Parent=Msa0968310;Name=Msa0968310-mRNA-1;_AED=0.02;_eAED=0.02;_QI=91\|1\|1\|1\|1\|1\|2\|232\|84
chr6_3	exon	98641951	98642095	98641951	ID=Msa0968310-mRNA-1:exon:18535;Parent=Msa0968310-mRNA-1
chr6_3	exon	98642396	98642828	98642396	ID=Msa0968310-mRNA-1:exon:18536;Parent=Msa0968310-mRNA-1
chr6_3	five_prime_UTR	98641951	98642041	98641951	ID=Msa0968310-mRNA-1:five_prime_utr;Parent=Msa0968310-mRNA-1
chr6_3	CDS	98642042	98642095	98642042	ID=Msa0968310-mRNA-1:cds;Parent=Msa0968310-mRNA-1
chr6_3	CDS	98642396	98642596	98642396	ID=Msa0968310-mRNA-1:cds;Parent=Msa0968310-mRNA-1
chr6_3	three_prime_UTR	98642597	98642828	98642597	ID=Msa0968310-mRNA-1:three_prime_utr;Parent=Msa0968310-mRNA-1

Gene Sequence	>Msa0968310 ATGGCCGATGACTTTGAATTCGGTGATAAAGTTCCTCCTTCCTTTGATCGCATGAAAGCTGGATCTCTAGGGTTCAACCCAGGCTTAATAGTTCTCCTGGTTATTGGTGGGTTGCTGTTGACATTCCTCATAGGAAATTATGCACTCTACGTTTATGCACAGAAGACCCTCCCTCCTAGAAAAAAGAAGCCAGTCTCAAAAAAGAAGATGAAAAAGGAGAGACTGAAGCAAGGAGTCTCTGCACCTGGAGAGTAG
Protein sequence	>Msa0968310 MADDFEFGDKVPPSFDRMKAGSLGFNPGLIVLLVIGGLLLTFLIGNYALYVYAQKTLPPRKKKPVSKKKMKKERLKQGVSAPGE*