Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0976630 | RHN50275.1 | 29.314 | 539 | 324 | 18 | 156 | 655 | 334 | 854 | 1.23e-22 | 116 |
| Msa0976630 | RHN50275.1 | 81.625 | 800 | 95 | 4 | 1 | 748 | 1 | 800 | 0.0 | 1229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0976630 | sp|Q6JN46|EIX2_SOLLC | 34.994 | 843 | 416 | 18 | 23 | 747 | 27 | 855 | 2.76e-126 | 404 |
| Msa0976630 | sp|Q6JN46|EIX2_SOLLC | 27.326 | 677 | 332 | 21 | 99 | 652 | 268 | 907 | 7.61e-31 | 133 |
| Msa0976630 | sp|Q6JN46|EIX2_SOLLC | 26.562 | 512 | 310 | 18 | 99 | 560 | 415 | 910 | 8.09e-18 | 92.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0976630 | A0A396HG56 | 29.314 | 539 | 324 | 18 | 156 | 655 | 334 | 854 | 5.90e-23 | 116 |
| Msa0976630 | A0A396HG56 | 81.625 | 800 | 95 | 4 | 1 | 748 | 1 | 800 | 0.0 | 1229 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0907320 | Msa0976630 | 0.823386 | 1.424474e-53 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0976630 | MtrunA17_Chr6g0455701 | 81.625 | 800 | 95 | 4 | 1 | 748 | 1 | 800 | 0.0 | 1229 |
| Msa0976630 | MtrunA17_Chr6g0455701 | 29.314 | 539 | 324 | 18 | 156 | 655 | 334 | 854 | 1.14e-26 | 116 |
| Msa0976630 | MtrunA17_Chr6g0455691 | 83.742 | 775 | 98 | 3 | 1 | 747 | 1 | 775 | 0.0 | 1210 |
| Msa0976630 | MtrunA17_Chr6g0455691 | 27.822 | 629 | 303 | 22 | 91 | 582 | 191 | 805 | 1.67e-22 | 103 |
| Msa0976630 | MtrunA17_Chr6g0455691 | 28.571 | 525 | 286 | 19 | 295 | 748 | 75 | 581 | 8.62e-13 | 72.0 |
| Msa0976630 | MtrunA17_Chr6g0455671 | 79.124 | 776 | 128 | 4 | 1 | 748 | 1 | 770 | 0.0 | 1159 |
| Msa0976630 | MtrunA17_Chr6g0455671 | 28.543 | 501 | 330 | 13 | 93 | 582 | 339 | 822 | 1.10e-21 | 100 |
| Msa0976630 | MtrunA17_Chr6g0455721 | 79.949 | 788 | 118 | 5 | 1 | 748 | 1 | 788 | 0.0 | 1155 |
| Msa0976630 | MtrunA17_Chr6g0455721 | 28.650 | 548 | 307 | 17 | 93 | 586 | 349 | 866 | 1.07e-21 | 100 |
| Msa0976630 | MtrunA17_Chr6g0455761 | 79.871 | 775 | 128 | 3 | 1 | 747 | 1 | 775 | 0.0 | 1147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0976630 | AT2G34930.1 | 33.255 | 848 | 426 | 23 | 30 | 747 | 32 | 869 | 3.19e-104 | 340 |
| Msa0976630 | AT2G34930.1 | 29.421 | 622 | 364 | 21 | 96 | 656 | 270 | 877 | 7.28e-35 | 143 |
| Msa0976630 | AT2G34930.1 | 25.788 | 539 | 348 | 12 | 85 | 589 | 361 | 881 | 2.00e-25 | 113 |
| Msa0976630 | AT2G34930.1 | 27.381 | 420 | 278 | 13 | 96 | 506 | 492 | 893 | 7.79e-17 | 85.5 |
| Msa0976630 | AT3G05660.1 | 32.334 | 767 | 398 | 26 | 31 | 748 | 30 | 724 | 6.95e-72 | 252 |
| Msa0976630 | AT3G11010.3 | 32.225 | 782 | 426 | 26 | 31 | 748 | 38 | 779 | 9.98e-72 | 252 |
| Msa0976630 | AT3G11010.3 | 30.153 | 524 | 282 | 20 | 104 | 566 | 339 | 839 | 2.58e-24 | 109 |
| Msa0976630 | AT3G11010.2 | 31.638 | 806 | 423 | 25 | 31 | 748 | 38 | 803 | 1.05e-71 | 252 |
| Msa0976630 | AT3G11010.2 | 30.153 | 524 | 282 | 20 | 104 | 566 | 363 | 863 | 2.98e-24 | 109 |
| Msa0976630 | AT4G13920.1 | 31.078 | 798 | 436 | 26 | 8 | 748 | 10 | 750 | 2.23e-71 | 251 |
Find 132 sgRNAs with CRISPR-Local
Find 342 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTTAAACTAAGAGGTG+AGG | 0.630238 | 6_4:+10806609 | None:intergenic |
| AAACAATCAACCTACGAAGA+TGG | 0.529333 | 6_4:+10806323 | None:intergenic |
| AACTTGCAATTAATTGATGT+TGG | 0.535546 | 6_4:-10806385 | Msa0976630:CDS |
| AAGGAAATATTGACATTGAA+AGG | 0.520101 | 6_4:-10806070 | Msa0976630:CDS |
| AAGGTCAAGAGTTTGTAAAT+TGG | 0.339448 | 6_4:+10807716 | None:intergenic |
| AAGGTGAAGAATGTTGCAAA+TGG | 0.525590 | 6_4:-10807980 | Msa0976630:CDS |
| AAGTGGTTCCATCAATGACA+AGG | 0.639575 | 6_4:-10806089 | Msa0976630:CDS |
| AAGTTGATAGAGTTGAAGCT+TGG | 0.616994 | 6_4:-10807786 | Msa0976630:CDS |
| AAGTTTGCCAGACAATTGAT+TGG | 0.451713 | 6_4:+10807461 | None:intergenic |
| AATTTACAAACTCTTGACCT+TGG | 0.490993 | 6_4:-10807714 | Msa0976630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGATAGCATCTCTAAATTTG+AGG | + | chr6_4:10806044-10806063 | None:intergenic | 30.0% | |
| GAGATGCTATCAATGTTAAT+AGG | - | chr6_4:10806052-10806071 | Msa0976630:CDS | 30.0% | |
| CAAGAGTTTGTAAATTGGAA+AGG | + | chr6_4:10806356-10806375 | None:intergenic | 30.0% | |
| AAGGTCAAGAGTTTGTAAAT+TGG | + | chr6_4:10806361-10806380 | None:intergenic | 30.0% | |
| AATTTACAAACTCTTGACCT+TGG | - | chr6_4:10806360-10806379 | Msa0976630:CDS | 30.0% | |
| GATTGACATATGATAGATCA+AGG | + | chr6_4:10806449-10806468 | None:intergenic | 30.0% | |
| CAGACAATTGATTGGAATTA+AGG | + | chr6_4:10806608-10806627 | None:intergenic | 30.0% | |
| CTTAATTCCAATCAATTGTC+TGG | - | chr6_4:10806606-10806625 | Msa0976630:CDS | 30.0% | |
| GTTCCGCAAAAAATGATTTA+AGG | - | chr6_4:10806658-10806677 | Msa0976630:CDS | 30.0% | |
| AAACATGAAAAATCTGGAAG+TGG | + | chr6_4:10806720-10806739 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 10805925 | 10808171 | 10805925 | ID=Msa0976630;Name=Msa0976630 |
| chr6_4 | mRNA | 10805925 | 10808171 | 10805925 | ID=Msa0976630-mRNA-1;Parent=Msa0976630;Name=Msa0976630-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|-1|0|1|-1|1|1|0|748 |
| chr6_4 | exon | 10805925 | 10808171 | 10805925 | ID=Msa0976630-mRNA-1:exon:3911;Parent=Msa0976630-mRNA-1 |
| chr6_4 | CDS | 10805925 | 10808171 | 10805925 | ID=Msa0976630-mRNA-1:cds;Parent=Msa0976630-mRNA-1 |
| Gene Sequence |
>Msa0976630 ATGCTTTATTGTGGGTATGATTTTCTATTTTGTTTGGTGGCCATTCTATGCATCAACTGGTCATGTGCAGAAGATTTTCATACAAACAAGTGTGTGGAGAAAGAGAGGAGAGCTCTCCTCAAATTTAGAGATGCTATCAATGTTAATAGGGATGGCATTTCATCATGGAAAGGTGAAGAATGTTGCAAATGGGAAGGAATTACATGTGATAATTTCACTCATCATGTAACCAGTTTGGAACTCTCTTTTGGATTGGGAGGTAAGTTAGATTCTTCAATATGTGAACTTCAACATCTTACTTCTTTAAATCTTGCTTATATTGGTTTAGAAGGAAAGATACCAAAGTGTATTGGTTCACTTGGTAAGTTGATAGAGTTGAAGCTTGGTGATAATTATTTTGTTGGTGTCATTCCTCCTAGTCTAGGAAACCTTTCCAATTTACAAACTCTTGACCTTGGAATGAATGATCACTTGACTGCAAATGACCTTGAATGGCTTTCTCATCTCTCTAATTTGAGATACCTTGATCTATCATATGTCAATCTCCATCAAGTCAATCCTAAGTCCATTCCCCTCCTGAATACTTCTTTTTCTCTGAAATCTCTCGATCTCACATATAATGAACTCCAGAGTAGCATACTAAAATCTTTTAGGAATATGTCTCAACTGCAAGTGTTATACCTTAATTCCAATCAATTGTCTGGCAAACTTAGTGACAACTTACAACAATTGTGTTCCGCAAAAAATGATTTAAGGAATTTGGATCTAAGCAATAACCCATTCAATGTGATGCCACTTCCAGATTTTTCATGTTTTCCATTTTTGGAGGCATTATATCTTGGAAATACCAATGTTGTTAGTCCATTTCCAAAATCACTTGTTCATTTGTCTTCCCTTTCATTTTTAGATCTCGGTTTTAACCAATTGAATGGATCACAACCATTACTTGAGATAACTAAACTCTCCTCATTAAAAACACTATATCTCTCCCATAATAACTTAAGTGGGCCATTTCCACATACAATTGGCCAACTTTCTCATCTAAATGAGTTACGTCTCTCTTCAAATAAGTTAAATGGCACCATTGATGAAACACATCTATCAAACTTATATGAATTGAAATATTTTGATGTGACTCAAAATTCACTTTCATTCAACTTGAGTTCAAAATGGGTTCCCCCTTTCAAACTAGAAACACTACATGCATCATCGTGCACATTGGGTCCTAAATTTCCATTATGGCTCAAACATCAAAGCGGGATTACTGATCTAAATATCTCTAATTGTGGTATTTCAGACACGTTTCCTAAATGGTTCTGGAATTTATCTTCAAGTTTGACATACTTGGATGTTTCACATAACAAATTCAATGGACCCTTGCCAAAATCATTACCAAGTTCGAAAGTAAATGATTATTATATCCGAGTGTGGGATTTTAGTTTCAACAATTTGAATGGTTCATTGCCTCCTTTTCCAAAGTTGGACGCTCTATTTCTCTCAAATAATATGTTTACAGGGTCCTTGTCTTCTTTTTGTACATCCTCACCTCTTAGTTTAAGTTTTTTGGACTTGTCTAGTAATTTGCTAGCAGGGAAACTTTCAGATTGTTGGAAGAAGTTTCAATCATTAGAAGTTCTAAATTTGGCAAATAACAATTTATCAGGAAAGTTGCCTAACTCACTTGGCGCTTTGCGACAAATTGAATCGCTTCATTTAAACAACAACAAATTCTCCGGTGAAATTCCATCTTTGATCCATTGTCACAACTTGCAATTAATTGATGTTGGTGATAACAATCTCCAAGGATCATTACCTATGTGGATAGGCCATCTTCGTAGGTTGATTGTTTTACGTCTGCGGGCAAATAAGTTCCAAGGAAGCATTCCTACAAGTATGTGCAATCTATCACTTCTTCAAATATTGGATCTCTCTCAAAACAATATAACAGGGGGAATAACACAATGCTTGGGTCACACAGTTGCTTTATCAAATTTGAAGTCTCCAAGAAACATATTTCACTATTGGTTAGCCTCGGGCTCTGATGGCAGTGAAAGTTATGAAAGTGGTTCCATCAATGACAAGGAAATATTGACATTGAAAGGATACAGCAGAGAATATGAGACAAATCTTGGATATTGGACAACCATTGATCTTTCTTGCAACCATTTAACAGGAGAGATACCCCAAAGTATAACAAAACTCGTGGCATTAGCTGGTTTAAACCTTTCATGGAATAATCTCACATGA |
| Protein sequence |
>Msa0976630 MLYCGYDFLFCLVAILCINWSCAEDFHTNKCVEKERRALLKFRDAINVNRDGISSWKGEECCKWEGITCDNFTHHVTSLELSFGLGGKLDSSICELQHLTSLNLAYIGLEGKIPKCIGSLGKLIELKLGDNYFVGVIPPSLGNLSNLQTLDLGMNDHLTANDLEWLSHLSNLRYLDLSYVNLHQVNPKSIPLLNTSFSLKSLDLTYNELQSSILKSFRNMSQLQVLYLNSNQLSGKLSDNLQQLCSAKNDLRNLDLSNNPFNVMPLPDFSCFPFLEALYLGNTNVVSPFPKSLVHLSSLSFLDLGFNQLNGSQPLLEITKLSSLKTLYLSHNNLSGPFPHTIGQLSHLNELRLSSNKLNGTIDETHLSNLYELKYFDVTQNSLSFNLSSKWVPPFKLETLHASSCTLGPKFPLWLKHQSGITDLNISNCGISDTFPKWFWNLSSSLTYLDVSHNKFNGPLPKSLPSSKVNDYYIRVWDFSFNNLNGSLPPFPKLDALFLSNNMFTGSLSSFCTSSPLSLSFLDLSSNLLAGKLSDCWKKFQSLEVLNLANNNLSGKLPNSLGALRQIESLHLNNNKFSGEIPSLIHCHNLQLIDVGDNNLQGSLPMWIGHLRRLIVLRLRANKFQGSIPTSMCNLSLLQILDLSQNNITGGITQCLGHTVALSNLKSPRNIFHYWLASGSDGSESYESGSINDKEILTLKGYSREYETNLGYWTTIDLSCNHLTGEIPQSITKLVALAGLNLSWNNLT* |
