AlfalfaGEDB | Msa0984260

M. sativa cultivar zhongmu-4 / Msa0984260

KEGG: K08269
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008589 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010646 GO:0010647 GO:0010648 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023056 GO:0023057 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045879 GO:0045880 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050794 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0984260	XP_003602555.1	92.683	287	13	1	2	280	4	290	0.0	548

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0984260	sp\|F4I1N8\|ATG1T_ARATH	56.863	255	102	1	23	269	7	261	3.54e-100	301

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0984260	G7JAY4	92.683	287	13	1	2	280	4	290	0.0	548

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0473780	Msa0984260	0.993798	2.673662e-202	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0984260	MtrunA17_Chr3g0129721	92.683	287	13	1	2	280	4	290	0.0	548
Msa0984260	MtrunA17_Chr4g0008561	49.807	259	121	2	23	273	19	276	9.37e-84	266
Msa0984260	MtrunA17_Chr8g0345801	45.714	245	125	1	23	259	15	259	1.69e-81	246
Msa0984260	MtrunA17_Chr5g0441011	37.308	260	138	6	23	267	13	262	1.27e-45	159
Msa0984260	MtrunA17_Chr3g0129701	90.411	73	7	0	181	253	14	86	3.75e-45	147
Msa0984260	MtrunA17_Chr8g0346071	34.091	264	153	6	23	274	17	271	4.82e-42	149
Msa0984260	MtrunA17_Chr5g0432491	36.226	265	148	6	23	274	23	279	5.05e-42	149
Msa0984260	MtrunA17_Chr2g0304111	33.212	274	158	6	16	274	16	279	1.15e-41	149
Msa0984260	MtrunA17_Chr1g0203881	32.443	262	165	5	23	274	41	300	2.14e-41	149
Msa0984260	MtrunA17_Chr5g0428031	34.831	267	150	7	23	273	9	267	2.43e-41	147

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0984260	AT1G49180.2	56.863	255	102	1	23	269	7	261	8.49e-102	302
Msa0984260	AT1G49180.1	56.863	255	102	1	23	269	7	261	3.60e-101	301
Msa0984260	AT2G37840.1	48.855	262	125	2	23	276	12	272	2.30e-84	268
Msa0984260	AT3G53930.1	48.092	262	127	2	23	276	20	280	1.54e-81	259
Msa0984260	AT3G53930.2	48.092	262	127	2	23	276	20	280	1.65e-81	259
Msa0984260	AT3G61960.2	46.538	260	131	1	23	274	10	269	1.45e-80	254
Msa0984260	AT3G61960.1	46.538	260	131	1	23	274	10	269	2.28e-80	254
Msa0984260	AT1G30270.2	35.206	267	148	6	23	274	31	287	8.03e-48	166
Msa0984260	AT1G30270.1	35.206	267	148	6	23	274	31	287	9.26e-48	166
Msa0984260	AT1G12580.1	35.249	261	157	5	23	273	44	302	1.22e-46	164

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Find 74 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACCTTCTCCAATTT+TGG	0.070296	6_4:-28096562	None:intergenic
AAAATTGATCTCACAGTCCA+AGG	0.551415	6_4:-28096679	None:intergenic
AAACCAGGGGATTGCTGTCA+AGG	0.499609	6_4:-28098539	None:intergenic
AAAGCAGAACAAAGACCAAG+TGG	0.701880	6_4:+28096597	Msa0984260:CDS
AACATTAGTTAAAATAACAT+CGG	0.621971	6_4:+28096400	None:intergenic
AAGTTGCTTCATGAATCTTC+TGG	0.468798	6_4:-28096926	None:intergenic
AATAAAATTCATCAAAAGAG+AGG	0.573975	6_4:-28098484	None:intergenic
AATAATTACAGGCAGACATG+TGG	0.624194	6_4:+28098089	Msa0984260:intron
AATATACATATAGTACATGA+AGG	0.607531	6_4:-28098818	None:intergenic
ACATTATTTCTGCCGTTAAA+AGG	0.253516	6_4:-28098154	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTCTGTCTTTGTAAAAATGT+TGG	+	chr6_4:28096435-28096454	Msa0984260:five_prime_UTR	30.0%
TTAAAGTCCAAAATTGGAGA+AGG	+	chr6_4:28096555-28096574	Msa0984260:CDS	30.0%
TGAAGAAGGTGAACAATATT+AGG	-	chr6_4:28096723-28096742	None:intergenic	30.0%
TCAAGGTAGTGTATAAGTTA+AGG	+	chr6_4:28097497-28097516	Msa0984260:intron	30.0%
CAAGGTAGTGTATAAGTTAA+GGG	+	chr6_4:28097498-28097517	Msa0984260:intron	30.0%
ATTTAGAAGCTCAAAAAGCA+TGG	-	chr6_4:28098125-28098144	None:intergenic	30.0%
TTAATATCAGCAGTAATCCT+AGG	-	chr6_4:28098225-28098244	None:intergenic	30.0%
TCTATTCAATCAACTACTTG+TGG	+	chr6_4:28098408-28098427	Msa0984260:intron	30.0%
GCTAAGGAAATAATAAAACC+AGG	-	chr6_4:28098557-28098576	None:intergenic	30.0%
ATGAAGTACATAGGATCAAT+AGG	-	chr6_4:28098852-28098871	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	28096414	28098921	28096414	ID=Msa0984260;Name=Msa0984260
chr6_4	mRNA	28096414	28098921	28096414	ID=Msa0984260-mRNA-1;Parent=Msa0984260;Name=Msa0984260-mRNA-1;_AED=0.14;_eAED=0.14;_QI=69\|0.85\|0.87\|1\|1\|1\|8\|377\|280
chr6_4	exon	28096414	28096752	28096414	ID=Msa0984260-mRNA-1:exon:7950;Parent=Msa0984260-mRNA-1
chr6_4	exon	28096832	28096949	28096832	ID=Msa0984260-mRNA-1:exon:7951;Parent=Msa0984260-mRNA-1
chr6_4	exon	28097088	28097146	28097088	ID=Msa0984260-mRNA-1:exon:7952;Parent=Msa0984260-mRNA-1
chr6_4	exon	28097437	28097491	28097437	ID=Msa0984260-mRNA-1:exon:7953;Parent=Msa0984260-mRNA-1
chr6_4	exon	28097629	28097708	28097629	ID=Msa0984260-mRNA-1:exon:7954;Parent=Msa0984260-mRNA-1
chr6_4	exon	28098100	28098180	28098100	ID=Msa0984260-mRNA-1:exon:7955;Parent=Msa0984260-mRNA-1
chr6_4	exon	28098260	28098371	28098260	ID=Msa0984260-mRNA-1:exon:7956;Parent=Msa0984260-mRNA-1
chr6_4	exon	28098477	28098921	28098477	ID=Msa0984260-mRNA-1:exon:7957;Parent=Msa0984260-mRNA-1
chr6_4	five_prime_UTR	28096414	28096482	28096414	ID=Msa0984260-mRNA-1:five_prime_utr;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28096483	28096752	28096483	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28096832	28096949	28096832	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28097088	28097146	28097088	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28097437	28097491	28097437	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28097629	28097708	28097629	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28098100	28098180	28098100	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28098260	28098371	28098260	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	CDS	28098477	28098544	28098477	ID=Msa0984260-mRNA-1:cds;Parent=Msa0984260-mRNA-1
chr6_4	three_prime_UTR	28098545	28098921	28098545	ID=Msa0984260-mRNA-1:three_prime_utr;Parent=Msa0984260-mRNA-1

Gene Sequence

>Msa0984260

ATGGAATATGATCGTGATTTTGAAGAAAAGTTGGTAAGAGAAGAAGGAAACAAAAAAAACTACTACTATGTTTTAAAGTCCAAAATTGGAGAAGGTTCTTTTTCCACAGTGTGGAAAGCAGAACAAAGACCAAGTGGTGAAGATGTTGCGGTGAAGCAAGTCTTTCTCTCTAAACTCAATCCTCGCCTCAAAGCTTCCTTGGACTGTGAGATCAATTTTTTGTCTTCTGTTAATCATCCTAATATTGTTCACCTTCTTCACTTCTTCCAGGGTAATGGATGTGTTTACTTGGTCCTTGAGTTTTGTGCTGGAGGGAATCTAGCTTCTTATATTCGATGCCATGGAAGAGTTCATCAACTAACCGCCAGAAGATTCATGAAGCAACTTGGCTCTGGTTTAAAAGTACTACACTCTCATGGCATCATTCATAGAGACTTGAAACCCGAGAACATTTTGCTATCAAGCCATGGTGCCGATGCAGTGCTGAAGATAGCTGATTTTGGTGAGTATGTTGAGACAGTTTGTGGAACTCCATCGTACATGGCTCCTGAAGTTCTTCAGTTCCAGAGATATGATCACAAGGCAGACATGTGGAGTGTCGGGGCCATGCTTTTTGAGCTTCTAAATGGTTATCCACCTTTTAACGGCAGAAATAATGTTCAGGTGTTGAAAAACATCAGATCATGTACCTGTCTTCCATTTTCTCAATCGATTCTTTATGGATTGGATTCTGCCTGTCTTGATATTTGTAAAAGACTACTGTGCTTAAATCCAGTTGAGCGCCTCTCTTTTGATGAATTTTATTTCCATAGCTTTCTGCGAGGAAAATCACTGGGACCTTGA

Protein sequence

>Msa0984260

MEYDRDFEEKLVREEGNKKNYYYVLKSKIGEGSFSTVWKAEQRPSGEDVAVKQVFLSKLNPRLKASLDCEINFLSSVNHPNIVHLLHFFQGNGCVYLVLEFCAGGNLASYIRCHGRVHQLTARRFMKQLGSGLKVLHSHGIIHRDLKPENILLSSHGADAVLKIADFGEYVETVCGTPSYMAPEVLQFQRYDHKADMWSVGAMLFELLNGYPPFNGRNNVQVLKNIRSCTCLPFSQSILYGLDSACLDICKRLLCLNPVERLSFDEFYFHSFLRGKSLGP*