Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0997160 | Msa0997170 | 0.807381 | 5.196731e-50 | -8.615850e-47 |
| Msa0997160 | Msa1111750 | 0.805064 | 1.598891e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTTTCATTAGGTTGTCTT+TGG | 0.265716 | 6_4:+76224633 | None:intergenic |
| CGTTCCGTTGACAAATTTAC+AGG | 0.346825 | 6_4:+76223905 | None:intergenic |
| ACATTCTGTTCAGTTTCATT+AGG | 0.355174 | 6_4:+76224623 | None:intergenic |
| AGTGGACAGTTCAATTTCAT+TGG | 0.382294 | 6_4:+76223871 | None:intergenic |
| TCTTTGAAAATCAAGTTAGA+AGG | 0.418814 | 6_4:+76223932 | None:intergenic |
| TTCATTAGGTTGTCTTTGGT+AGG | 0.467818 | 6_4:+76224637 | None:intergenic |
| TCATTAGGTTGTCTTTGGTA+GGG | 0.479170 | 6_4:+76224638 | None:intergenic |
| TCAAGTTAGAAGGTACATCT+TGG | 0.484761 | 6_4:+76223942 | None:intergenic |
| ATGCACAAACAAGCTAGTAT+TGG | 0.526027 | 6_4:+76224679 | None:intergenic |
| AGCAGATGTAAGTTGTTCAG+TGG | 0.618860 | 6_4:+76223853 | None:intergenic |
| TGGACCTGTAAATTTGTCAA+CGG | 0.637837 | 6_4:-76223909 | Msa0997160:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTCATTTTGTATAAAAC+TGG | + | chr6_4:76224023-76224042 | None:intergenic | 15.0% |
| !!! | ATGTTTTAATGATAGTAATA+AGG | - | chr6_4:76224374-76224393 | Msa0997160:intron | 15.0% |
| !!! | ATTTTCATTTTGTATAAAAC+TGG | + | chr6_4:76224023-76224042 | None:intergenic | 15.0% |
| !!! | ATGTTTTAATGATAGTAATA+AGG | - | chr6_4:76224374-76224393 | Msa0997160:intron | 15.0% |
| !!! | TTTTGAATGTGTTTTGAAAA+TGG | - | chr6_4:76223843-76223862 | Msa0997160:CDS | 20.0% |
| !!! | TTTGAATGTGTTTTGAAAAT+GGG | - | chr6_4:76223844-76223863 | Msa0997160:CDS | 20.0% |
| !! | TGCATAAAACTTGTAAAAAA+AGG | + | chr6_4:76224120-76224139 | None:intergenic | 20.0% |
| !! | CAAATCATATTATTCAAATC+AGG | + | chr6_4:76224210-76224229 | None:intergenic | 20.0% |
| !!! | CTGATTTGAATAATATGATT+TGG | - | chr6_4:76224208-76224227 | Msa0997160:intron | 20.0% |
| !!! | TGATAGTGTTTTTTTCTTTT+TGG | - | chr6_4:76224238-76224257 | Msa0997160:intron | 20.0% |
| !!! | TTTTTTTCTTTTTGGAATAC+CGG | - | chr6_4:76224246-76224265 | Msa0997160:intron | 20.0% |
| !!! | AGAAAGTAAATGTGATTTTT+TGG | - | chr6_4:76224486-76224505 | Msa0997160:intron | 20.0% |
| !!! | TTTTGAATGTGTTTTGAAAA+TGG | - | chr6_4:76223843-76223862 | Msa0997160:CDS | 20.0% |
| !!! | TTTGAATGTGTTTTGAAAAT+GGG | - | chr6_4:76223844-76223863 | Msa0997160:CDS | 20.0% |
| !! | TGCATAAAACTTGTAAAAAA+AGG | + | chr6_4:76224120-76224139 | None:intergenic | 20.0% |
| !! | CAAATCATATTATTCAAATC+AGG | + | chr6_4:76224210-76224229 | None:intergenic | 20.0% |
| !!! | CTGATTTGAATAATATGATT+TGG | - | chr6_4:76224208-76224227 | Msa0997160:intron | 20.0% |
| !!! | TGATAGTGTTTTTTTCTTTT+TGG | - | chr6_4:76224238-76224257 | Msa0997160:intron | 20.0% |
| !!! | TTTTTTTCTTTTTGGAATAC+CGG | - | chr6_4:76224246-76224265 | Msa0997160:intron | 20.0% |
| !!! | AGAAAGTAAATGTGATTTTT+TGG | - | chr6_4:76224486-76224505 | Msa0997160:intron | 20.0% |
| ! | AAATTTAACAGTTGCGAATT+TGG | - | chr6_4:76224039-76224058 | Msa0997160:intron | 25.0% |
| !! | TGCGAATTTGGAAAATTTTA+AGG | - | chr6_4:76224051-76224070 | Msa0997160:intron | 25.0% |
| ! | ATAACAAAAAAATGGCAGTT+TGG | + | chr6_4:76224077-76224096 | None:intergenic | 25.0% |
| ! | ACAGCATCATAACAAAAAAA+TGG | + | chr6_4:76224085-76224104 | None:intergenic | 25.0% |
| !!! | AAATGTGATTTTTTGGAAAC+TGG | - | chr6_4:76224493-76224512 | Msa0997160:intron | 25.0% |
| ! | AAAATAAATATAGCAGAACC+AGG | + | chr6_4:76224554-76224573 | None:intergenic | 25.0% |
| !! | TCCTTTTGAATGTTGATATA+AGG | - | chr6_4:76224610-76224629 | Msa0997160:intron | 25.0% |
| !! | CCTTTTGAATGTTGATATAA+GGG | - | chr6_4:76224611-76224630 | Msa0997160:intron | 25.0% |
| !! | CTTTTGAATGTTGATATAAG+GGG | - | chr6_4:76224612-76224631 | Msa0997160:intron | 25.0% |
| !! | TCTTTGAAAATCAAGTTAGA+AGG | + | chr6_4:76224657-76224676 | None:intergenic | 25.0% |
| !! | TCTAACTTGATTTTCAAAGA+TGG | - | chr6_4:76224657-76224676 | Msa0997160:CDS | 25.0% |
| ! | AAATTTAACAGTTGCGAATT+TGG | - | chr6_4:76224039-76224058 | Msa0997160:intron | 25.0% |
| !! | TGCGAATTTGGAAAATTTTA+AGG | - | chr6_4:76224051-76224070 | Msa0997160:intron | 25.0% |
| ! | ATAACAAAAAAATGGCAGTT+TGG | + | chr6_4:76224077-76224096 | None:intergenic | 25.0% |
| ! | ACAGCATCATAACAAAAAAA+TGG | + | chr6_4:76224085-76224104 | None:intergenic | 25.0% |
| !!! | AAATGTGATTTTTTGGAAAC+TGG | - | chr6_4:76224493-76224512 | Msa0997160:intron | 25.0% |
| ! | AAAATAAATATAGCAGAACC+AGG | + | chr6_4:76224554-76224573 | None:intergenic | 25.0% |
| !! | TCCTTTTGAATGTTGATATA+AGG | - | chr6_4:76224610-76224629 | Msa0997160:intron | 25.0% |
| !! | CCTTTTGAATGTTGATATAA+GGG | - | chr6_4:76224611-76224630 | Msa0997160:intron | 25.0% |
| !! | CTTTTGAATGTTGATATAAG+GGG | - | chr6_4:76224612-76224631 | Msa0997160:intron | 25.0% |
| !! | TCTTTGAAAATCAAGTTAGA+AGG | + | chr6_4:76224657-76224676 | None:intergenic | 25.0% |
| !! | TCTAACTTGATTTTCAAAGA+TGG | - | chr6_4:76224657-76224676 | Msa0997160:CDS | 25.0% |
| ACATTCTGTTCAGTTTCATT+AGG | + | chr6_4:76223966-76223985 | None:intergenic | 30.0% | |
| TGTCTAAACTAAGATCCAAT+GGG | + | chr6_4:76224172-76224191 | None:intergenic | 30.0% | |
| TTGTCTAAACTAAGATCCAA+TGG | + | chr6_4:76224173-76224192 | None:intergenic | 30.0% | |
| ATTGGATCTTAGTTTAGACA+AGG | - | chr6_4:76224172-76224191 | Msa0997160:intron | 30.0% | |
| TTGGATCTTAGTTTAGACAA+GGG | - | chr6_4:76224173-76224192 | Msa0997160:intron | 30.0% | |
| CTGAAAATAAAACACACTAC+CGG | + | chr6_4:76224268-76224287 | None:intergenic | 30.0% | |
| GTTTACATTTACCTACCATA+TGG | + | chr6_4:76224335-76224354 | None:intergenic | 30.0% | |
| AAAGAATAGCAGTTGTCAAA+AGG | + | chr6_4:76224528-76224547 | None:intergenic | 30.0% | |
| ! | TTTTGACAACTGCTATTCTT+TGG | - | chr6_4:76224527-76224546 | Msa0997160:intron | 30.0% |
| TTTGACAACTGCTATTCTTT+GGG | - | chr6_4:76224528-76224547 | Msa0997160:intron | 30.0% | |
| CCCTTATATCAACATTCAAA+AGG | + | chr6_4:76224614-76224633 | None:intergenic | 30.0% | |
| ACATTCTGTTCAGTTTCATT+AGG | + | chr6_4:76223966-76223985 | None:intergenic | 30.0% | |
| TGTCTAAACTAAGATCCAAT+GGG | + | chr6_4:76224172-76224191 | None:intergenic | 30.0% | |
| TTGTCTAAACTAAGATCCAA+TGG | + | chr6_4:76224173-76224192 | None:intergenic | 30.0% | |
| ATTGGATCTTAGTTTAGACA+AGG | - | chr6_4:76224172-76224191 | Msa0997160:intron | 30.0% | |
| TTGGATCTTAGTTTAGACAA+GGG | - | chr6_4:76224173-76224192 | Msa0997160:intron | 30.0% | |
| CTGAAAATAAAACACACTAC+CGG | + | chr6_4:76224268-76224287 | None:intergenic | 30.0% | |
| GTTTACATTTACCTACCATA+TGG | + | chr6_4:76224335-76224354 | None:intergenic | 30.0% | |
| AAAGAATAGCAGTTGTCAAA+AGG | + | chr6_4:76224528-76224547 | None:intergenic | 30.0% | |
| ! | TTTTGACAACTGCTATTCTT+TGG | - | chr6_4:76224527-76224546 | Msa0997160:intron | 30.0% |
| TTTGACAACTGCTATTCTTT+GGG | - | chr6_4:76224528-76224547 | Msa0997160:intron | 30.0% | |
| CCCTTATATCAACATTCAAA+AGG | + | chr6_4:76224614-76224633 | None:intergenic | 30.0% | |
| ATGCACAAACAAGCTAGTAT+TGG | + | chr6_4:76223910-76223929 | None:intergenic | 35.0% | |
| ! | TCATTAGGTTGTCTTTGGTA+GGG | + | chr6_4:76223951-76223970 | None:intergenic | 35.0% |
| ! | TTCATTAGGTTGTCTTTGGT+AGG | + | chr6_4:76223952-76223971 | None:intergenic | 35.0% |
| ! | CAGTTTCATTAGGTTGTCTT+TGG | + | chr6_4:76223956-76223975 | None:intergenic | 35.0% |
| GGACAAGAAACAAAGATGAA+CGG | + | chr6_4:76224151-76224170 | None:intergenic | 35.0% | |
| ! | ATCTTTGTTTCTTGTCCCAT+TGG | - | chr6_4:76224154-76224173 | Msa0997160:intron | 35.0% |
| TGGATCTTAGTTTAGACAAG+GGG | - | chr6_4:76224174-76224193 | Msa0997160:intron | 35.0% | |
| ! | CTGTGATTTGTGATTCCATA+TGG | - | chr6_4:76224317-76224336 | Msa0997160:intron | 35.0% |
| GATTTGTGATTCCATATGGT+AGG | - | chr6_4:76224321-76224340 | Msa0997160:intron | 35.0% | |
| AGCGTTAAGCATGTTATACT+TGG | - | chr6_4:76224439-76224458 | Msa0997160:intron | 35.0% | |
| ! | TCAAGTTAGAAGGTACATCT+TGG | + | chr6_4:76224647-76224666 | None:intergenic | 35.0% |
| TGGACCTGTAAATTTGTCAA+CGG | - | chr6_4:76224677-76224696 | Msa0997160:CDS | 35.0% | |
| AGTGGACAGTTCAATTTCAT+TGG | + | chr6_4:76224718-76224737 | None:intergenic | 35.0% | |
| ATGCACAAACAAGCTAGTAT+TGG | + | chr6_4:76223910-76223929 | None:intergenic | 35.0% | |
| ! | TCATTAGGTTGTCTTTGGTA+GGG | + | chr6_4:76223951-76223970 | None:intergenic | 35.0% |
| ! | TTCATTAGGTTGTCTTTGGT+AGG | + | chr6_4:76223952-76223971 | None:intergenic | 35.0% |
| ! | CAGTTTCATTAGGTTGTCTT+TGG | + | chr6_4:76223956-76223975 | None:intergenic | 35.0% |
| GGACAAGAAACAAAGATGAA+CGG | + | chr6_4:76224151-76224170 | None:intergenic | 35.0% | |
| ! | ATCTTTGTTTCTTGTCCCAT+TGG | - | chr6_4:76224154-76224173 | Msa0997160:intron | 35.0% |
| TGGATCTTAGTTTAGACAAG+GGG | - | chr6_4:76224174-76224193 | Msa0997160:intron | 35.0% | |
| ! | CTGTGATTTGTGATTCCATA+TGG | - | chr6_4:76224317-76224336 | Msa0997160:intron | 35.0% |
| GATTTGTGATTCCATATGGT+AGG | - | chr6_4:76224321-76224340 | Msa0997160:intron | 35.0% | |
| AGCGTTAAGCATGTTATACT+TGG | - | chr6_4:76224439-76224458 | Msa0997160:intron | 35.0% | |
| ! | TCAAGTTAGAAGGTACATCT+TGG | + | chr6_4:76224647-76224666 | None:intergenic | 35.0% |
| TGGACCTGTAAATTTGTCAA+CGG | - | chr6_4:76224677-76224696 | Msa0997160:CDS | 35.0% | |
| AGTGGACAGTTCAATTTCAT+TGG | + | chr6_4:76224718-76224737 | None:intergenic | 35.0% | |
| AGCATAGAGTACATGAGCAT+AGG | + | chr6_4:76224299-76224318 | None:intergenic | 40.0% | |
| CGTTCCGTTGACAAATTTAC+AGG | + | chr6_4:76224684-76224703 | None:intergenic | 40.0% | |
| AGCAGATGTAAGTTGTTCAG+TGG | + | chr6_4:76224736-76224755 | None:intergenic | 40.0% | |
| AGCATAGAGTACATGAGCAT+AGG | + | chr6_4:76224299-76224318 | None:intergenic | 40.0% | |
| CGTTCCGTTGACAAATTTAC+AGG | + | chr6_4:76224684-76224703 | None:intergenic | 40.0% | |
| AGCAGATGTAAGTTGTTCAG+TGG | + | chr6_4:76224736-76224755 | None:intergenic | 40.0% | |
| CAACTGCTATTCTTTGGGCC+TGG | - | chr6_4:76224533-76224552 | Msa0997160:intron | 50.0% | |
| CAACTGCTATTCTTTGGGCC+TGG | - | chr6_4:76224533-76224552 | Msa0997160:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 76223838 | 76224770 | 76223838 | ID=Msa0997160;Name=Msa0997160 |
| chr6_4 | mRNA | 76223838 | 76224770 | 76223838 | ID=Msa0997160-mRNA-1;Parent=Msa0997160;Name=Msa0997160-mRNA-1;_AED=0.57;_eAED=0.75;_QI=0|0|0|1|0|0|2|0|94 |
| chr6_4 | exon | 76224625 | 76224770 | 76224625 | ID=Msa0997160-mRNA-1:exon:14358;Parent=Msa0997160-mRNA-1 |
| chr6_4 | exon | 76223838 | 76223976 | 76223838 | ID=Msa0997160-mRNA-1:exon:14357;Parent=Msa0997160-mRNA-1 |
| chr6_4 | CDS | 76224625 | 76224770 | 76224625 | ID=Msa0997160-mRNA-1:cds;Parent=Msa0997160-mRNA-1 |
| chr6_4 | CDS | 76223838 | 76223976 | 76223838 | ID=Msa0997160-mRNA-1:cds;Parent=Msa0997160-mRNA-1 |
| Gene Sequence |
| Protein sequence |
