AlfalfaGEDB | Msa1000560

M. sativa cultivar zhongmu-4 / Msa1000560

KEGG: K19477
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF00481.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1000560	XP_003620565.2	95.122	1107	19	2	1	1107	1	1072	0.0	2151

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1000560	sp\|Q9SL76\|P2C19_ARATH	64.505	1141	324	14	1	1107	1	1094	0.0	1444

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1000560	G7KNY7	95.122	1107	19	2	1	1107	1	1072	0.0	2151

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0911050	Msa1000560	0.847195	1.348267e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1000560	MtrunA17_Chr6g0486661	95.122	1107	19	2	1	1107	1	1072	0.0	2151
Msa1000560	MtrunA17_Chr4g0066371	58.258	333	109	4	80	412	42	344	4.50e-125	388
Msa1000560	MtrunA17_Chr2g0286951	58.006	331	109	3	80	410	43	343	1.48e-122	382
Msa1000560	MtrunA17_Chr7g0261491	28.249	354	196	11	790	1090	114	462	1.66e-34	140
Msa1000560	MtrunA17_Chr1g0183651	27.811	338	191	9	790	1078	114	447	2.05e-34	140
Msa1000560	MtrunA17_Chr1g0167311	26.984	378	221	10	765	1091	83	456	1.95e-33	136
Msa1000560	MtrunA17_Chr4g0059751	37.209	172	103	3	890	1058	191	360	7.38e-33	132
Msa1000560	MtrunA17_Chr1g0167351	28.571	322	173	10	809	1078	123	439	2.80e-32	133
Msa1000560	MtrunA17_Chr7g0245031	27.419	372	240	10	706	1058	52	412	3.28e-32	132
Msa1000560	MtrunA17_Chr1g0187581	27.726	321	176	10	809	1078	130	445	1.26e-31	131

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1000560	AT2G20050.1	64.505	1141	324	14	1	1107	1	1094	0.0	1444
Msa1000560	AT2G20050.2	64.417	1141	322	15	1	1107	1	1091	0.0	1438
Msa1000560	AT3G06270.1	55.556	333	118	3	80	412	44	346	1.21e-117	367
Msa1000560	AT3G23310.1	25.954	393	233	10	752	1091	76	463	8.57e-34	138
Msa1000560	AT4G14350.2	28.135	327	178	11	809	1084	132	452	1.65e-32	134
Msa1000560	AT4G14350.1	28.135	327	178	11	809	1084	132	452	1.65e-32	134
Msa1000560	AT4G14350.4	28.135	327	178	11	809	1084	132	452	1.65e-32	134
Msa1000560	AT4G14350.3	28.135	327	178	11	809	1084	132	452	1.68e-32	134
Msa1000560	AT5G26010.1	33.871	248	132	5	106	342	56	282	3.89e-32	129
Msa1000560	AT3G08730.1	37.566	189	108	5	890	1072	229	413	9.16e-32	130

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Find 257 sgRNAs with CRISPR-Local

Find 578 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATGGGGAGGTACCTA+GGG	0.581768	6_4:-83495719	Msa1000560:CDS
AAAAGTCACTCATGGTTTAA+CGG	0.515952	6_4:-83491997	Msa1000560:CDS
AAAAGTTATACTTTGCAGGG+TGG	0.716025	6_4:-83496541	Msa1000560:CDS
AAAATCAAATTCTATAATCT+GGG	0.500194	6_4:-83499829	Msa1000560:intron
AAAGAGAAAGATTTGATTAA+GGG	0.304516	6_4:-83493610	Msa1000560:CDS
AAAGAGTTAACGTCGAACCT+GGG	0.598587	6_4:-83496733	Msa1000560:CDS
AACAACTGCTATAACGATTT+TGG	0.168530	6_4:-83499191	Msa1000560:CDS
AACAGAAGCAGCACAATATC+GGG	0.478712	6_4:+83493453	None:intergenic
AACATCATCCTCCGCAACAG+CGG	0.653868	6_4:+83494595	None:intergenic
AACATTCACAATTTGTGGGA+GGG	0.559694	6_4:-83492920	Msa1000560:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAGACACTTAGTTAACAT+AGG	+	chr6_4:83491436-83491455	None:intergenic	30.0%
CAAATCAAATTCTGTCATCT+GGG	-	chr6_4:83491484-83491503	Msa1000560:three_prime_UTR	30.0%
GCAAATCAAATTCTATGATC+TGG	-	chr6_4:83491619-83491638	Msa1000560:three_prime_UTR	30.0%
TATTGATCAGACACCTTATA+GGG	-	chr6_4:83492509-83492528	Msa1000560:CDS	30.0%
CTTACTTTGGATCAAATTGA+AGG	-	chr6_4:83492573-83492592	Msa1000560:intron	30.0%
AGTTCAAAACTAACAATCTC+CGG	+	chr6_4:83492756-83492775	None:intergenic	30.0%
AAGAAAGAAAGTTGTTGGAA+TGG	-	chr6_4:83492973-83492992	Msa1000560:intron	30.0%
TTGTCAATTGCGAAAAATAG+TGG	-	chr6_4:83493027-83493046	Msa1000560:intron	30.0%
ATTAATCCGCAATACAATAG+CGG	+	chr6_4:83493093-83493112	None:intergenic	30.0%
TGATTTAAGTAATCAGGAAC+TGG	-	chr6_4:83493161-83493180	Msa1000560:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	83491260	83500339	83491260	ID=Msa1000560;Name=Msa1000560
chr6_4	mRNA	83491260	83500339	83491260	ID=Msa1000560-mRNA-1;Parent=Msa1000560;Name=Msa1000560-mRNA-1;_AED=0.04;_eAED=0.04;_QI=513\|0.82\|0.88\|0.94\|0.88\|0.83\|18\|568\|1107
chr6_4	exon	83491260	83492073	83491260	ID=Msa1000560-mRNA-1:exon:16225;Parent=Msa1000560-mRNA-1
chr6_4	exon	83492399	83492555	83492399	ID=Msa1000560-mRNA-1:exon:16224;Parent=Msa1000560-mRNA-1
chr6_4	exon	83492862	83492983	83492862	ID=Msa1000560-mRNA-1:exon:16223;Parent=Msa1000560-mRNA-1
chr6_4	exon	83493256	83493321	83493256	ID=Msa1000560-mRNA-1:exon:16222;Parent=Msa1000560-mRNA-1
chr6_4	exon	83493426	83493703	83493426	ID=Msa1000560-mRNA-1:exon:16221;Parent=Msa1000560-mRNA-1
chr6_4	exon	83494020	83494086	83494020	ID=Msa1000560-mRNA-1:exon:16220;Parent=Msa1000560-mRNA-1
chr6_4	exon	83494164	83494239	83494164	ID=Msa1000560-mRNA-1:exon:16219;Parent=Msa1000560-mRNA-1
chr6_4	exon	83494518	83494831	83494518	ID=Msa1000560-mRNA-1:exon:16218;Parent=Msa1000560-mRNA-1
chr6_4	exon	83494907	83495138	83494907	ID=Msa1000560-mRNA-1:exon:16217;Parent=Msa1000560-mRNA-1
chr6_4	exon	83495664	83495746	83495664	ID=Msa1000560-mRNA-1:exon:16216;Parent=Msa1000560-mRNA-1
chr6_4	exon	83496483	83496569	83496483	ID=Msa1000560-mRNA-1:exon:16215;Parent=Msa1000560-mRNA-1
chr6_4	exon	83496715	83496840	83496715	ID=Msa1000560-mRNA-1:exon:16214;Parent=Msa1000560-mRNA-1
chr6_4	exon	83497217	83497447	83497217	ID=Msa1000560-mRNA-1:exon:16213;Parent=Msa1000560-mRNA-1
chr6_4	exon	83497561	83497692	83497561	ID=Msa1000560-mRNA-1:exon:16212;Parent=Msa1000560-mRNA-1
chr6_4	exon	83498284	83498364	83498284	ID=Msa1000560-mRNA-1:exon:16211;Parent=Msa1000560-mRNA-1
chr6_4	exon	83498745	83499797	83498745	ID=Msa1000560-mRNA-1:exon:16210;Parent=Msa1000560-mRNA-1
chr6_4	exon	83499830	83499859	83499830	ID=Msa1000560-mRNA-1:exon:16209;Parent=Msa1000560-mRNA-1
chr6_4	exon	83499884	83500339	83499884	ID=Msa1000560-mRNA-1:exon:16208;Parent=Msa1000560-mRNA-1
chr6_4	five_prime_UTR	83499884	83500339	83499884	ID=Msa1000560-mRNA-1:five_prime_utr;Parent=Msa1000560-mRNA-1
chr6_4	five_prime_UTR	83499830	83499859	83499830	ID=Msa1000560-mRNA-1:five_prime_utr;Parent=Msa1000560-mRNA-1
chr6_4	five_prime_UTR	83499771	83499797	83499771	ID=Msa1000560-mRNA-1:five_prime_utr;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83498745	83499770	83498745	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83498284	83498364	83498284	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83497561	83497692	83497561	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83497217	83497447	83497217	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83496715	83496840	83496715	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83496483	83496569	83496483	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83495664	83495746	83495664	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83494907	83495138	83494907	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83494518	83494831	83494518	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83494164	83494239	83494164	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83494020	83494086	83494020	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83493426	83493703	83493426	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83493256	83493321	83493256	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83492862	83492983	83492862	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83492399	83492555	83492399	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	CDS	83491828	83492073	83491828	ID=Msa1000560-mRNA-1:cds;Parent=Msa1000560-mRNA-1
chr6_4	three_prime_UTR	83491260	83491827	83491260	ID=Msa1000560-mRNA-1:three_prime_utr;Parent=Msa1000560-mRNA-1

Gene Sequence

>Msa1000560

ATGGGATGCATTTATTCAAGAGTATGCATAGGTGAAACATGTAAAGGTTCAAGCATCAATGGTGACCCAATTTCAAGACATGAGCTTCAACAAGCTTTTTCACCATCCTCTGATGGTGGTGAAATTCGAGACCAATTGAATCAGTTAAGCTTAACTAGAGATCCTGAAGCTGGAATAAGAAGACTAGCAAGGGTTTCGTCGCAGTTTCTTCCACCGGATGGTTCGAGGATTGCGAAGGTTGATTCGGAGAAGTATGAATTACGGTATTCGTATCTTTCTCAGCGAGGTTATTATCCTGATGCTCTTGATAAAGCAAATCAAGATAGTTTTTGTATTCACACGCCGTTTGGAACGGATGTGAATGATCATTTTTTCGGTGTTTTTGATGGTCATGGTGAATTTGGGGCGGAGTGTTCGCAGTTTGTGAAGCGTAGGCTTTGTGAGAATTTATTGAGGAATTCTAAGTTTAATGTTGATCCTGTTGAGGCTTGTCATGCGGCTTTTTTAGCTACGAATTCGCAGTTACATGCTGATGTTTTAGATGATAGTATGAGTGGAACAACTGCTATAACGATTTTGGTTAGAGGGAAGACGGTTTATGTAGCGAATTGTGGTGATTCGAGGGCGGTTATAGCGGAGAAGAAAGGGAAAGATGGTGATATTGTTGCAGTTGATTTGTCTATTGATCAGACACCTTATAGGGTCGATGAGTTGGAAAGAGTGAAACTTTGTGGTGCTAGGGTTCTTACTTTGGATCAAATTGAAGGGTTAAAGAATCCTGAAGTTCCGTGTTGGGGTAGCGATGAAGAAGGTGATGATGGTGATCCTCCTAGGTTGTGGGTCCCAAATGGAATGTACCCTGGTACTGCTTTTACAAGGAGTATTGGTGATTCTATTGCCGAGACTATTGGTGTTGTTGCTAATCCGGAGATTGTTAGTTTTGAACTCACACCTAATAATCCATTTTTTGTTATTGCTAGTGATGGAGTTTTTGAGTTTCTCTCAAGCCAAACTGTGGTTGATATGGAAGTAGGATTTAGGAATGAGAAATTTTCGCTTACCATTATGAGAAGCACTTATGCAACAAGTGACTCTTTTGGAAAGCAAGTTGCAAAATTTAAAGATCCACGTGATGCTTGTGCAGCGATAGTGGCAGAATCTTATCGACTATGGCTACAGTACGAGACTCGTACAGATGATATTACAGTCATCATTGTGCATATAAATGGACTAAATGAACCTGCTGTCGCTCACTCAGAAAGTTATCGTGAAATTTTACGAACCCCTGTCCCTCAAGTTGTAGAGGTGACAGGTTCAGAATCTCCTTCCACATTTGGATGGAATGCTAGGAATCATCGTGTAAGGCAAGAATTGTCAAGGGCACGCCTCCGAGCAATTGAAAATTCCCTCGAGAATGGACAAGGTTGGGTTCCTCCATCTTCAGCTCACAGAAAGACATGGGAGGAAGAAGCACACATAGAGCAGGCGTTGAATGATCATTTTCTCTTTCGAAAACTTACCGAATCTCAGTGTCATGTTTTATTGGATTGCATGCAAAGAGTTAACGTCGAACCTGGGGATATTATAGTCAAACAGAGTGACAAAAGTTATACTTTGCAGGGTGGTGAAAGTGACTGTTTTTATGTTGTTGGCAGCGGAGAATTTGAAGTCTTGGCAACTCAGGAAGAAAAAGATGGGGAGGTACCTAGGGTTTTGCAGCGCTACACAGCTGAAAAACTATCATGCTTTGGAGAACTTGCTCTGATGTACAATAAACCACTCCAGGCCTCTGTGCGTGCGGTAACAAGCGGAACTCTTTGGACTTTAAAGCGAGAAGATTTTCGCGGGATTTTAGTGTCAGAATTCTCTAACTTATCATCATTGAAGTTACTTCGGTCCGTAGATCTCCTCTCCAGATTGTCCATTTTACAACTGAGTCAGATCTCCGACTCCCTTTCTGAAGTTACCTTCTCAAGTGGACAGACAATAATCAATAAAAATGAAGTCCTTGCATTGTACATCATCCAAAAGGGACGTGTAAAATTAACTTTCGATGCTGATTTATTGACAAGTCCAAATACCTACAGCCTCAAGTCCGACATCGAAAATGAGGACGATGATGTACAGAGCGGAACAGAACTGTCAATAGAGAAGCCGGAGGGAAGCTATTTCGGTGAATGGTCCCTTCTTGATCAACACATTGGCCCCTTAACCGCTGTTGCGGAGGATGATGTTGAATGTGCTTTACTAACAAAGGACAAACTTGAATCAGTTATTGGCTCCTTGCAAAAGATTTCTCAGGAAGATCACAAGTTGAGGGATAATTCCGAGGAATTGAATAAAAGCTTTGAATTCTCATCGTTTGATAAAGTCCAGCTCTCAGATTTGGAGTGGAGAAAGACGTTATATTCCACTGACTGCTCTGAGATTGGACTTGCAAATTTAAAAGACTCAGAAAATGTGCTTACTTTGAAGAAATTTTCCAAGCCGAAAGTGAAAAGGCTAGGAAAGGAATCACAAGTCTTGAAAGAGAAAGATTTGATTAAGGGTATGAGCTCCTCAGCTTGTGTACCGCAAGTTTTATGCACCTTTGCCGACCGCATATATGCTGGAATACTGCTAAATACACGTCTTGCCTGCCCTTTGTCTTCCATACTATCAACTCCTTTTAGCGAATCCGCGGCCCGATATTGTGCTGCTTCTGTTGTGATTGCCTTAGAAGACTTGCACAAGAATGGTGTTCTGTATAGAGGTGTCTCGCATGATGTTTTGATGTTGGATCAAAGAGGACGTATACAGTTGGTGGACTTCAGATTTGGAAAGAAGCTCTCTGATGAGAGAACATTCACAATTTGTGGGAGGGCAGATTCACTAGCTCCAGAGATTGTTCTTGGAAAAGGCCACAGTTTCCCTGCCGACTGGTGGGCATTGGGAGTTTTGGTATATTTTATGTTATGTGGTGAAATGCCATTTGGATCATGGAGAGAAAATGAACTCGATACAGTTGCCAAAATTGCAAAAAGGAAACTAAATCTTCCTGAAACTTTTAGCCCTGACACTGTTGATCTCATCTCCAAGTTACTTGATGTCGAAGAAAATACTAGACTTGGTAGCCAAGGTTCTGATTCTGTCAAAAGTCACTCATGGTTTAACGGTATCGACTGGGAAGCTCTTAGACACCATACTTTTCCCGTTCCTCCAGAAATTAGTTCTCGTATAACACAATACTTGGAAGTTCGTTACGAGGATTCTTCTGCTTCTGCAGAGTCTCCATTAGAAGAGGTAGAAGAACTTAATGTGCCTGAATGGCTTGAAGATTGGTAG

Protein sequence

>Msa1000560

MGCIYSRVCIGETCKGSSINGDPISRHELQQAFSPSSDGGEIRDQLNQLSLTRDPEAGIRRLARVSSQFLPPDGSRIAKVDSEKYELRYSYLSQRGYYPDALDKANQDSFCIHTPFGTDVNDHFFGVFDGHGEFGAECSQFVKRRLCENLLRNSKFNVDPVEACHAAFLATNSQLHADVLDDSMSGTTAITILVRGKTVYVANCGDSRAVIAEKKGKDGDIVAVDLSIDQTPYRVDELERVKLCGARVLTLDQIEGLKNPEVPCWGSDEEGDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIVSFELTPNNPFFVIASDGVFEFLSSQTVVDMEVGFRNEKFSLTIMRSTYATSDSFGKQVAKFKDPRDACAAIVAESYRLWLQYETRTDDITVIIVHINGLNEPAVAHSESYREILRTPVPQVVEVTGSESPSTFGWNARNHRVRQELSRARLRAIENSLENGQGWVPPSSAHRKTWEEEAHIEQALNDHFLFRKLTESQCHVLLDCMQRVNVEPGDIIVKQSDKSYTLQGGESDCFYVVGSGEFEVLATQEEKDGEVPRVLQRYTAEKLSCFGELALMYNKPLQASVRAVTSGTLWTLKREDFRGILVSEFSNLSSLKLLRSVDLLSRLSILQLSQISDSLSEVTFSSGQTIINKNEVLALYIIQKGRVKLTFDADLLTSPNTYSLKSDIENEDDDVQSGTELSIEKPEGSYFGEWSLLDQHIGPLTAVAEDDVECALLTKDKLESVIGSLQKISQEDHKLRDNSEELNKSFEFSSFDKVQLSDLEWRKTLYSTDCSEIGLANLKDSENVLTLKKFSKPKVKRLGKESQVLKEKDLIKGMSSSACVPQVLCTFADRIYAGILLNTRLACPLSSILSTPFSESAARYCAASVVIALEDLHKNGVLYRGVSHDVLMLDQRGRIQLVDFRFGKKLSDERTFTICGRADSLAPEIVLGKGHSFPADWWALGVLVYFMLCGEMPFGSWRENELDTVAKIAKRKLNLPETFSPDTVDLISKLLDVEENTRLGSQGSDSVKSHSWFNGIDWEALRHHTFPVPPEISSRITQYLEVRYEDSSASAESPLEEVEELNVPEWLEDW*