Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006670 | GAU25981.1 | 81.967 | 122 | 22 | 0 | 1 | 122 | 371 | 492 | 8.36e-62 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006670 | sp|Q9LP24|Y1571_ARATH | 42.391 | 92 | 48 | 2 | 1 | 90 | 1031 | 1119 | 1.09e-16 | 78.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006670 | A0A2Z6M1H9 | 81.967 | 122 | 22 | 0 | 1 | 122 | 371 | 492 | 3.99e-62 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0902020 | Msa1006670 | 0.812650 | 3.809688e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006670 | MtrunA17_Chr7g0217061 | 83.607 | 122 | 20 | 0 | 1 | 122 | 669 | 790 | 1.86e-65 | 211 |
| Msa1006670 | MtrunA17_Chr7g0217081 | 81.148 | 122 | 23 | 0 | 1 | 122 | 746 | 867 | 1.62e-61 | 201 |
| Msa1006670 | MtrunA17_Chr7g0215681 | 80.328 | 122 | 24 | 0 | 1 | 122 | 666 | 787 | 3.04e-60 | 197 |
| Msa1006670 | MtrunA17_Chr7g0215061 | 77.869 | 122 | 27 | 0 | 1 | 122 | 707 | 828 | 1.01e-58 | 193 |
| Msa1006670 | MtrunA17_Chr7g0215651 | 77.049 | 122 | 28 | 0 | 1 | 122 | 619 | 740 | 1.22e-58 | 192 |
| Msa1006670 | MtrunA17_Chr7g0215641 | 75.410 | 122 | 30 | 0 | 1 | 122 | 653 | 774 | 9.31e-58 | 190 |
| Msa1006670 | MtrunA17_Chr7g0215631 | 75.410 | 122 | 30 | 0 | 1 | 122 | 628 | 749 | 1.36e-57 | 189 |
| Msa1006670 | MtrunA17_Chr7g0215671 | 76.230 | 122 | 29 | 0 | 1 | 122 | 635 | 756 | 1.14e-52 | 176 |
| Msa1006670 | MtrunA17_Chr8g0357171 | 77.869 | 122 | 27 | 0 | 1 | 122 | 831 | 952 | 3.47e-52 | 176 |
| Msa1006670 | MtrunA17_Chr8g0357191 | 77.049 | 122 | 28 | 0 | 1 | 122 | 875 | 996 | 7.03e-52 | 175 |
| Msa1006670 | MtrunA17_Chr7g0218991 | 71.311 | 122 | 35 | 0 | 1 | 122 | 642 | 763 | 1.28e-51 | 173 |
| Msa1006670 | MtrunA17_Chr7g0218871 | 68.333 | 120 | 38 | 0 | 1 | 120 | 105 | 224 | 2.18e-51 | 162 |
| Msa1006670 | MtrunA17_Chr7g0215011 | 75.833 | 120 | 29 | 0 | 1 | 120 | 666 | 785 | 1.14e-49 | 168 |
| Msa1006670 | MtrunA17_Chr8g0357151 | 76.230 | 122 | 29 | 0 | 1 | 122 | 959 | 1080 | 1.37e-49 | 168 |
| Msa1006670 | MtrunA17_Chr7g0250431 | 51.456 | 103 | 41 | 3 | 1 | 97 | 92 | 191 | 1.11e-24 | 93.2 |
| Msa1006670 | MtrunA17_Chr7g0217041 | 41.284 | 109 | 64 | 0 | 3 | 111 | 705 | 813 | 1.52e-23 | 94.4 |
| Msa1006670 | MtrunA17_Chr7g0250401 | 51.456 | 103 | 41 | 3 | 1 | 97 | 487 | 586 | 2.03e-23 | 94.0 |
| Msa1006670 | MtrunA17_Chr7g0250491 | 53.684 | 95 | 39 | 2 | 1 | 91 | 964 | 1057 | 2.35e-23 | 94.0 |
| Msa1006670 | MtrunA17_Chr1g0165191 | 54.737 | 95 | 33 | 3 | 1 | 88 | 1056 | 1147 | 4.80e-23 | 92.8 |
| Msa1006670 | MtrunA17_Chr5g0439291 | 45.536 | 112 | 56 | 2 | 3 | 110 | 922 | 1032 | 4.99e-23 | 92.8 |
| Msa1006670 | MtrunA17_Chr1g0165181 | 52.688 | 93 | 34 | 3 | 3 | 88 | 194 | 283 | 5.38e-23 | 90.5 |
| Msa1006670 | MtrunA17_Chr7g0250381 | 46.087 | 115 | 53 | 3 | 1 | 109 | 949 | 1060 | 8.51e-22 | 89.4 |
| Msa1006670 | MtrunA17_Chr7g0250351 | 49.515 | 103 | 43 | 3 | 1 | 97 | 741 | 840 | 8.62e-22 | 89.4 |
| Msa1006670 | MtrunA17_Chr4g0019371 | 46.667 | 90 | 47 | 1 | 1 | 90 | 848 | 936 | 1.30e-21 | 89.0 |
| Msa1006670 | MtrunA17_Chr7g0232171 | 52.222 | 90 | 37 | 3 | 3 | 90 | 997 | 1082 | 1.33e-21 | 89.0 |
| Msa1006670 | MtrunA17_Chr1g0165221 | 48.515 | 101 | 39 | 3 | 1 | 91 | 1032 | 1129 | 3.99e-21 | 87.4 |
| Msa1006670 | MtrunA17_Chr4g0019041 | 45.455 | 88 | 47 | 1 | 1 | 88 | 853 | 939 | 4.98e-21 | 87.0 |
| Msa1006670 | MtrunA17_Chr7g0232131 | 50.000 | 90 | 42 | 2 | 3 | 90 | 1092 | 1180 | 5.01e-21 | 87.0 |
| Msa1006670 | MtrunA17_Chr1g0165981 | 46.875 | 96 | 43 | 1 | 4 | 91 | 479 | 574 | 7.28e-21 | 86.7 |
| Msa1006670 | MtrunA17_Chr8g0355401 | 48.421 | 95 | 39 | 3 | 1 | 88 | 63 | 154 | 7.39e-21 | 82.4 |
| Msa1006670 | MtrunA17_Chr7g0250471 | 43.966 | 116 | 56 | 3 | 1 | 110 | 947 | 1059 | 7.85e-21 | 86.7 |
| Msa1006670 | MtrunA17_Chr7g0231541 | 50.000 | 90 | 42 | 2 | 3 | 90 | 625 | 713 | 7.89e-21 | 86.7 |
| Msa1006670 | MtrunA17_Chr1g0165951 | 47.525 | 101 | 40 | 3 | 1 | 91 | 905 | 1002 | 8.28e-21 | 86.7 |
| Msa1006670 | MtrunA17_Chr1g0165261 | 46.535 | 101 | 41 | 3 | 1 | 91 | 497 | 594 | 8.32e-21 | 86.7 |
| Msa1006670 | MtrunA17_Chr7g0232211 | 48.889 | 90 | 43 | 2 | 3 | 90 | 985 | 1073 | 2.77e-20 | 85.1 |
| Msa1006670 | MtrunA17_Chr1g0165211 | 47.525 | 101 | 40 | 3 | 1 | 91 | 1075 | 1172 | 2.87e-20 | 85.1 |
| Msa1006670 | MtrunA17_Chr1g0165141 | 50.526 | 95 | 37 | 3 | 1 | 88 | 1033 | 1124 | 5.43e-20 | 84.3 |
| Msa1006670 | MtrunA17_Chr1g0165971 | 47.525 | 101 | 40 | 3 | 1 | 91 | 960 | 1057 | 6.14e-20 | 84.0 |
| Msa1006670 | MtrunA17_Chr1g0165231 | 46.535 | 101 | 41 | 3 | 1 | 91 | 1058 | 1155 | 6.67e-20 | 84.0 |
| Msa1006670 | MtrunA17_Chr1g0165301 | 46.535 | 101 | 41 | 3 | 1 | 91 | 1058 | 1155 | 6.67e-20 | 84.0 |
| Msa1006670 | MtrunA17_Chr1g0165891 | 45.545 | 101 | 42 | 3 | 1 | 91 | 575 | 672 | 1.40e-19 | 83.2 |
| Msa1006670 | MtrunA17_Chr7g0232451 | 49.485 | 97 | 43 | 3 | 1 | 92 | 906 | 1001 | 2.62e-19 | 82.4 |
| Msa1006670 | MtrunA17_Chr1g0165901 | 46.535 | 101 | 41 | 3 | 1 | 91 | 1143 | 1240 | 3.77e-19 | 82.0 |
| Msa1006670 | MtrunA17_Chr7g0261331 | 42.609 | 115 | 57 | 4 | 1 | 109 | 1108 | 1219 | 4.61e-19 | 81.6 |
| Msa1006670 | MtrunA17_Chr7g0232631 | 48.454 | 97 | 44 | 3 | 1 | 92 | 898 | 993 | 6.58e-19 | 81.3 |
| Msa1006670 | MtrunA17_Chr7g0216271 | 78.571 | 42 | 9 | 0 | 1 | 42 | 1 | 42 | 1.55e-17 | 70.9 |
| Msa1006670 | MtrunA17_Chr7g0233841 | 44.898 | 98 | 41 | 3 | 1 | 88 | 216 | 310 | 7.16e-17 | 74.7 |
| Msa1006670 | MtrunA17_Chr1g0165281 | 43.878 | 98 | 45 | 3 | 1 | 91 | 824 | 918 | 6.23e-16 | 72.8 |
| Msa1006670 | MtrunA17_Chr1g0165241 | 43.878 | 98 | 45 | 3 | 1 | 91 | 824 | 918 | 6.23e-16 | 72.8 |
| Msa1006670 | MtrunA17_Chr1g0165311 | 43.878 | 98 | 45 | 3 | 1 | 91 | 824 | 918 | 6.23e-16 | 72.8 |
| Msa1006670 | MtrunA17_Chr7g0233881 | 41.414 | 99 | 48 | 2 | 1 | 90 | 848 | 945 | 4.84e-15 | 70.1 |
| Msa1006670 | MtrunA17_Chr7g0217101 | 100.000 | 30 | 0 | 0 | 1 | 30 | 501 | 530 | 3.87e-14 | 67.4 |
| Msa1006670 | MtrunA17_Chr7g0232611 | 46.341 | 82 | 38 | 3 | 1 | 77 | 100 | 180 | 4.09e-14 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1006670 | AT1G35710.1 | 42.391 | 92 | 48 | 2 | 1 | 90 | 1031 | 1119 | 1.11e-17 | 78.2 |
| Msa1006670 | AT4G08850.1 | 41.758 | 91 | 51 | 2 | 1 | 90 | 955 | 1044 | 8.46e-17 | 75.5 |
Find 19 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGACACACACTTCATTTC+TGG | 0.121979 | 7_1:-5450508 | Msa1006660:CDS |
| AACTAAAACAATCATGTTAT+TGG | 0.192505 | 7_1:-5450425 | Msa1006660:CDS |
| CCACTCAAAGGAATGCTTAA+TGG | 0.256958 | 7_1:-5450553 | Msa1006660:CDS |
| AGTGGCACTTGAAACTTTAA+TGG | 0.283958 | 7_1:+5450309 | Msa1006670:CDS |
| ACTAAAACAATCATGTTATT+GGG | 0.383181 | 7_1:-5450424 | Msa1006660:CDS |
| ACAATCATGTTATTGGGAAA+TGG | 0.389994 | 7_1:-5450418 | Msa1006660:CDS |
| TATATTGTCATCACTTCAAT+TGG | 0.416298 | 7_1:+5450348 | Msa1006670:CDS |
| GTGGCACTTGAAACTTTAAT+GGG | 0.449262 | 7_1:+5450310 | Msa1006670:CDS |
| AAATGTGATGTATATAGCTT+TGG | 0.479124 | 7_1:+5450283 | Msa1006670:CDS |
| TTCATTTCTGGTCGAGAGCG+AGG | 0.513389 | 7_1:-5450496 | Msa1006660:CDS |
| TGTATATAGCTTTGGAGTAG+TGG | 0.533127 | 7_1:+5450291 | Msa1006670:CDS |
| CCATTAAGCATTCCTTTGAG+TGG | 0.533842 | 7_1:+5450553 | Msa1006670:CDS |
| TAAGCAAGCAAATGCGACTA+CGG | 0.548524 | 7_1:-5450464 | Msa1006660:CDS |
| ACTTTAATGGGAAGACACCC+TGG | 0.558199 | 7_1:+5450322 | Msa1006670:CDS |
| TCATTTCTGGTCGAGAGCGA+GGG | 0.559593 | 7_1:-5450495 | Msa1006660:CDS |
| TGCACTGAAATTCCACTCAA+AGG | 0.575318 | 7_1:-5450565 | Msa1006660:CDS |
| AGTGATGACAATATATCACC+AGG | 0.591738 | 7_1:-5450340 | Msa1006660:intron |
| TATTGGGAAATGGAAGACGT+TGG | 0.666264 | 7_1:-5450408 | Msa1006660:intron |
| GTGATGACAATATATCACCA+GGG | 0.698225 | 7_1:-5450339 | Msa1006660:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAACATTGTTTCATATT+GGG | + | chr7_1:5450608-5450627 | Msa1006670:CDS | 15.0% |
| !! | ACTAAAACAATCATGTTATT+GGG | - | chr7_1:5450427-5450446 | Msa1006660:CDS | 20.0% |
| !! | AACTAAAACAATCATGTTAT+TGG | - | chr7_1:5450428-5450447 | Msa1006660:CDS | 20.0% |
| !! | CTAAAAACATTGTTTCATAT+TGG | + | chr7_1:5450607-5450626 | Msa1006670:CDS | 20.0% |
| ! | AAATGTGATGTATATAGCTT+TGG | + | chr7_1:5450283-5450302 | Msa1006670:CDS | 25.0% |
| ! | TATATTGTCATCACTTCAAT+TGG | + | chr7_1:5450348-5450367 | Msa1006670:CDS | 25.0% |
| ACAATCATGTTATTGGGAAA+TGG | - | chr7_1:5450421-5450440 | Msa1006660:CDS | 30.0% | |
| !! | TGTATATAGCTTTGGAGTAG+TGG | + | chr7_1:5450291-5450310 | Msa1006670:CDS | 35.0% |
| AGTGGCACTTGAAACTTTAA+TGG | + | chr7_1:5450309-5450328 | Msa1006670:CDS | 35.0% | |
| GTGGCACTTGAAACTTTAAT+GGG | + | chr7_1:5450310-5450329 | Msa1006670:CDS | 35.0% | |
| GTGATGACAATATATCACCA+GGG | - | chr7_1:5450342-5450361 | Msa1006660:intron | 35.0% | |
| AGTGATGACAATATATCACC+AGG | - | chr7_1:5450343-5450362 | Msa1006660:intron | 35.0% | |
| TATTGGGAAATGGAAGACGT+TGG | - | chr7_1:5450411-5450430 | Msa1006660:intron | 40.0% | |
| TAAGCAAGCAAATGCGACTA+CGG | - | chr7_1:5450467-5450486 | Msa1006660:CDS | 40.0% | |
| TTCGACACACACTTCATTTC+TGG | - | chr7_1:5450511-5450530 | Msa1006660:CDS | 40.0% | |
| CCACTCAAAGGAATGCTTAA+TGG | - | chr7_1:5450556-5450575 | Msa1006660:CDS | 40.0% | |
| CCATTAAGCATTCCTTTGAG+TGG | + | chr7_1:5450553-5450572 | Msa1006670:CDS | 40.0% | |
| TGCACTGAAATTCCACTCAA+AGG | - | chr7_1:5450568-5450587 | Msa1006660:CDS | 40.0% | |
| ACTTTAATGGGAAGACACCC+TGG | + | chr7_1:5450322-5450341 | Msa1006670:CDS | 45.0% | |
| !! | TCATTTCTGGTCGAGAGCGA+GGG | - | chr7_1:5450498-5450517 | Msa1006660:CDS | 50.0% |
| !! | TTCATTTCTGGTCGAGAGCG+AGG | - | chr7_1:5450499-5450518 | Msa1006660:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 5450268 | 5450639 | 5450268 | ID=Msa1006670;Name=Msa1006670 |
| chr7_1 | mRNA | 5450268 | 5450639 | 5450268 | ID=Msa1006670-mRNA-1;Parent=Msa1006670;Name=Msa1006670-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|123 |
| chr7_1 | exon | 5450268 | 5450639 | 5450268 | ID=Msa1006670-mRNA-1:exon:1765;Parent=Msa1006670-mRNA-1 |
| chr7_1 | CDS | 5450268 | 5450639 | 5450268 | ID=Msa1006670-mRNA-1:cds;Parent=Msa1006670-mRNA-1 |
| Gene Sequence |
| Protein sequence |
