Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007530 | XP_003621282.2 | 75.862 | 145 | 16 | 2 | 1 | 127 | 1 | 144 | 8.61e-28 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007530 | G7L237 | 75.862 | 145 | 16 | 2 | 1 | 127 | 1 | 144 | 4.11e-28 | 112 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1007530 | Msa1136290 | 0.804714 | 1.892152e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007530 | MtrunA17_Chr7g0217851 | 70.175 | 114 | 16 | 1 | 32 | 127 | 5 | 118 | 3.37e-22 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTTTCTTCTTCTTAGACT+TGG | 0.357409 | 7_1:-7068036 | None:intergenic |
| TTATGATCAGATTTATCATC+AGG | 0.427079 | 7_1:+7068198 | Msa1007530:CDS |
| CTCTTATTCTAAATTCACTC+TGG | 0.436351 | 7_1:+7068648 | Msa1007530:CDS |
| TTATGTCTAAGTAATGTTGT+GGG | 0.494117 | 7_1:-7070570 | None:intergenic |
| TTTATGTCTAAGTAATGTTG+TGG | 0.498207 | 7_1:-7070571 | None:intergenic |
| TCGTTCGTTCCCATGGGTGG+AGG | 0.527930 | 7_1:+7067987 | None:intergenic |
| AGCCTCTTCTGCTAATCGAC+GGG | 0.530366 | 7_1:-7068519 | None:intergenic |
| ACAGGCCCGCAAAGCAAAAG+AGG | 0.538823 | 7_1:+7068613 | Msa1007530:CDS |
| AACTCGTTCGTTCCCATGGG+TGG | 0.547111 | 7_1:+7067984 | None:intergenic |
| AGCCCGTCGATTAGCAAAAG+AGG | 0.550604 | 7_1:+7068571 | Msa1007530:CDS |
| AGCCCGTCGATTAGCAGAAG+AGG | 0.554670 | 7_1:+7068517 | Msa1007530:CDS |
| TCTTATTCTAAATTCACTCT+GGG | 0.558794 | 7_1:+7068649 | Msa1007530:CDS |
| CTGTGTTTGTAATGTTTGCA+AGG | 0.567137 | 7_1:-7068736 | None:intergenic |
| TCGTGTGTGGGCAGTAAAGA+CGG | 0.585395 | 7_1:-7068691 | None:intergenic |
| GAGCCTCTTCTGCTAATCGA+CGG | 0.589148 | 7_1:-7068520 | None:intergenic |
| GTGTTTCTCTAGTCGTGTGT+GGG | 0.609787 | 7_1:-7068703 | None:intergenic |
| AGTGTTTCTCTAGTCGTGTG+TGG | 0.609826 | 7_1:-7068704 | None:intergenic |
| ACGGTGCATTGCATCAGCAG+TGG | 0.666153 | 7_1:-7068672 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CGTAAAATAAAAATTTATTT+TGG | - | chr7_1:7069037-7069056 | None:intergenic | 10.0% |
| !! | TTACTTAAAAAAAATGTAAT+AGG | + | chr7_1:7069355-7069374 | Msa1007530:intron | 10.0% |
| !! | TATGATATGAATTGTTAATT+TGG | + | chr7_1:7068335-7068354 | Msa1007530:intron | 15.0% |
| !! | AAATACAAATACAAATACAA+TGG | - | chr7_1:7068449-7068468 | None:intergenic | 15.0% |
| !! | ATAATTTAATTCTATTACAG+AGG | + | chr7_1:7068912-7068931 | Msa1007530:intron | 15.0% |
| !! | AAAAAAAAAAACAAAGAACT+AGG | + | chr7_1:7069594-7069613 | Msa1007530:intron | 15.0% |
| !!! | CTTTAAAAAAAGAAAATGAA+TGG | + | chr7_1:7069739-7069758 | Msa1007530:intron | 15.0% |
| !!! | TTTTAACACTCTAATTATAT+GGG | + | chr7_1:7070380-7070399 | Msa1007530:intron | 15.0% |
| !! | CTAGTAAATGTTATGAAAAT+TGG | - | chr7_1:7068112-7068131 | None:intergenic | 20.0% |
| !!! | TTTTAACTAACTTGTTATGT+TGG | + | chr7_1:7068230-7068249 | Msa1007530:intron | 20.0% |
| !! | ATAAAATGATACTACTAATC+TGG | + | chr7_1:7068276-7068295 | Msa1007530:intron | 20.0% |
| !!! | ATGAATTGTTAATTTGGTTA+TGG | + | chr7_1:7068341-7068360 | Msa1007530:intron | 20.0% |
| !! | GTATTTGTATTTGTATTTGT+AGG | + | chr7_1:7068451-7068470 | Msa1007530:intron | 20.0% |
| !! | ATTATAGACCAAATAGTTAA+GGG | - | chr7_1:7068853-7068872 | None:intergenic | 20.0% |
| !! | AATTATAGACCAAATAGTTA+AGG | - | chr7_1:7068854-7068873 | None:intergenic | 20.0% |
| !! | TATGCGTATGAAAAAATATT+TGG | + | chr7_1:7068886-7068905 | Msa1007530:intron | 20.0% |
| !! | ATAGCTATAGATTAACAATT+TGG | - | chr7_1:7069332-7069351 | None:intergenic | 20.0% |
| !! | ACTACTAATTCAATTGTATT+AGG | + | chr7_1:7069858-7069877 | Msa1007530:intron | 20.0% |
| !!! | TTGTTATGTAATATTTTGTG+AGG | - | chr7_1:7069884-7069903 | None:intergenic | 20.0% |
| !!! | CTTTTAACACTCTAATTATA+TGG | + | chr7_1:7070379-7070398 | Msa1007530:intron | 20.0% |
| !! | GAATCTTAATGTTATCATAT+TGG | + | chr7_1:7070506-7070525 | Msa1007530:intron | 20.0% |
| !!! | ATGTTATCATATTGGTTAAT+AGG | + | chr7_1:7070514-7070533 | Msa1007530:intron | 20.0% |
| !!! | ACTTGGATTTCTTTTTTGTT+CGG | - | chr7_1:7068022-7068041 | None:intergenic | 25.0% |
| ! | ATAAATAGATAGATAGAGAG+AGG | - | chr7_1:7068070-7068089 | None:intergenic | 25.0% |
| ! | TTATGATCAGATTTATCATC+AGG | + | chr7_1:7068198-7068217 | Msa1007530:CDS | 25.0% |
| ! | TAAAAAAAAAGCAGAAGAAG+AGG | + | chr7_1:7068547-7068566 | Msa1007530:CDS | 25.0% |
| ! | TCTTATTCTAAATTCACTCT+GGG | + | chr7_1:7068649-7068668 | Msa1007530:CDS | 25.0% |
| !!! | GCAATTTTTAAGAAAACCTA+CGG | - | chr7_1:7068762-7068781 | None:intergenic | 25.0% |
| !! | GTTTTCTTAAAAATTGCTCT+AGG | + | chr7_1:7068765-7068784 | Msa1007530:intron | 25.0% |
| !! | GCTTTAACTTCAAATGTTAA+AGG | + | chr7_1:7069110-7069129 | Msa1007530:intron | 25.0% |
| ! | CACATAATAGTTAAGTTCTT+TGG | - | chr7_1:7069211-7069230 | None:intergenic | 25.0% |
| ! | AAGAACTTAACTATTATGTG+CGG | + | chr7_1:7069211-7069230 | Msa1007530:intron | 25.0% |
| ! | TGTAATAGGTAATTGAGAAT+TGG | + | chr7_1:7069369-7069388 | Msa1007530:intron | 25.0% |
| !!! | GTAGCATGACATTTTTTTTT+AGG | - | chr7_1:7069558-7069577 | None:intergenic | 25.0% |
| ! | AATCGAGATCTTCAATTTAA+AGG | + | chr7_1:7069797-7069816 | Msa1007530:intron | 25.0% |
| ! | ATATATTTGCTTGAGAAACA+AGG | + | chr7_1:7070200-7070219 | Msa1007530:intron | 25.0% |
| ! | TATATTTGCTTGAGAAACAA+GGG | + | chr7_1:7070201-7070220 | Msa1007530:intron | 25.0% |
| !! | TATGCTTTTATAGAGAGAAT+CGG | + | chr7_1:7070233-7070252 | Msa1007530:intron | 25.0% |
| AATGATACTACTAATCTGGA+TGG | + | chr7_1:7068280-7068299 | Msa1007530:intron | 30.0% | |
| ! | TAACATGATGATGATGATCT+CGG | + | chr7_1:7068377-7068396 | Msa1007530:intron | 30.0% |
| TCTTGCTCAAAAAAAACTAC+AGG | + | chr7_1:7068595-7068614 | Msa1007530:CDS | 30.0% | |
| CTCTTATTCTAAATTCACTC+TGG | + | chr7_1:7068648-7068667 | Msa1007530:CDS | 30.0% | |
| TTATAAAAATGCTATGCCAG+TGG | + | chr7_1:7069065-7069084 | Msa1007530:intron | 30.0% | |
| GCTATAGATTAACAATTTGG+AGG | - | chr7_1:7069329-7069348 | None:intergenic | 30.0% | |
| ATTGGTTAATCTCTCAAAGT+AGG | + | chr7_1:7069387-7069406 | Msa1007530:intron | 30.0% | |
| TATTAAAATTAAAGCTGCCG+TGG | + | chr7_1:7070340-7070359 | Msa1007530:intron | 30.0% | |
| ATTAAAATTAAAGCTGCCGT+GGG | + | chr7_1:7070341-7070360 | Msa1007530:intron | 30.0% | |
| !! | ACTACTCTAGAAATAGCTTT+TGG | + | chr7_1:7070460-7070479 | Msa1007530:intron | 30.0% |
| !! | CTACTCTAGAAATAGCTTTT+GGG | + | chr7_1:7070461-7070480 | Msa1007530:intron | 30.0% |
| CAGTTTCTTCTTCTTAGACT+TGG | - | chr7_1:7068039-7068058 | None:intergenic | 35.0% | |
| !!! | CTCTTCTTCTGCTACTTTTT+TGG | - | chr7_1:7068495-7068514 | None:intergenic | 35.0% |
| CAAAAAAGTAGCAGAAGAAG+AGG | + | chr7_1:7068493-7068512 | Msa1007530:CDS | 35.0% | |
| CTGTGTTTGTAATGTTTGCA+AGG | - | chr7_1:7068739-7068758 | None:intergenic | 35.0% | |
| !! | CGTTGTTTCTTAGTAAGGAA+AGG | - | chr7_1:7068801-7068820 | None:intergenic | 35.0% |
| !! | AAGCACGTTGTTTCTTAGTA+AGG | - | chr7_1:7068806-7068825 | None:intergenic | 35.0% |
| TGATCACAAAAACAAGGTTG+TGG | + | chr7_1:7069088-7069107 | Msa1007530:intron | 35.0% | |
| !!! | TTTTCCATGTACTGGTTTTG+GGG | - | chr7_1:7069286-7069305 | None:intergenic | 35.0% |
| !!! | GTTTTCCATGTACTGGTTTT+GGG | - | chr7_1:7069287-7069306 | None:intergenic | 35.0% |
| !!! | AGTTTTCCATGTACTGGTTT+TGG | - | chr7_1:7069288-7069307 | None:intergenic | 35.0% |
| !! | GAATTAGTAGTCTTGTCCTT+GGG | - | chr7_1:7069849-7069868 | None:intergenic | 35.0% |
| !! | TGAATTAGTAGTCTTGTCCT+TGG | - | chr7_1:7069850-7069869 | None:intergenic | 35.0% |
| TGATGTAATTGTCAAGCATG+AGG | + | chr7_1:7069984-7070003 | Msa1007530:intron | 35.0% | |
| ! | AATACCTATGCATCTAGCTT+AGG | + | chr7_1:7070421-7070440 | Msa1007530:intron | 35.0% |
| TTATCCTAAGCTAGATGCAT+AGG | - | chr7_1:7070428-7070447 | None:intergenic | 35.0% | |
| AACATTACAAACACAGCCGT+AGG | + | chr7_1:7068743-7068762 | Msa1007530:intron | 40.0% | |
| CTTTGACGCCCTTAACTATT+TGG | + | chr7_1:7068842-7068861 | Msa1007530:intron | 40.0% | |
| !!! | CTTGTTTTTGTGATCACCAC+TGG | - | chr7_1:7069084-7069103 | None:intergenic | 40.0% |
| CAGTGGTGATCACAAAAACA+AGG | + | chr7_1:7069082-7069101 | Msa1007530:intron | 40.0% | |
| GCATTGCATATATACCCATG+TGG | - | chr7_1:7069682-7069701 | None:intergenic | 40.0% | |
| TAAGCATGTGCTTCGTACTA+TGG | - | chr7_1:7070315-7070334 | None:intergenic | 40.0% | |
| ATGATCTCGGCTCTCTGTAT+AGG | + | chr7_1:7068390-7068409 | Msa1007530:intron | 45.0% | |
| ! | AGCCTCTTTTGCTAATCGAC+GGG | - | chr7_1:7068576-7068595 | None:intergenic | 45.0% |
| ! | GAGCCTCTTTTGCTAATCGA+CGG | - | chr7_1:7068577-7068596 | None:intergenic | 45.0% |
| GTGTTTCTCTAGTCGTGTGT+GGG | - | chr7_1:7068706-7068725 | None:intergenic | 45.0% | |
| AGTGTTTCTCTAGTCGTGTG+TGG | - | chr7_1:7068707-7068726 | None:intergenic | 45.0% | |
| ! | GTATTTTGCATGCGCAACTG+CGG | - | chr7_1:7068946-7068965 | None:intergenic | 45.0% |
| CAAACCCCAAAACCAGTACA+TGG | + | chr7_1:7069279-7069298 | Msa1007530:intron | 45.0% | |
| ! | CTGGCAAGTTTTCCATGTAC+TGG | - | chr7_1:7069294-7069313 | None:intergenic | 45.0% |
| TCTACAGAGGAGATCCACAT+GGG | + | chr7_1:7069665-7069684 | Msa1007530:intron | 45.0% | |
| TTGCTTGTCGTCACTAGAGT+CGG | + | chr7_1:7069823-7069842 | Msa1007530:intron | 45.0% | |
| !! | AAAAGCCGCTTGTTGTGAGA+GGG | - | chr7_1:7070136-7070155 | None:intergenic | 45.0% |
| !! | AAAAAGCCGCTTGTTGTGAG+AGG | - | chr7_1:7070137-7070156 | None:intergenic | 45.0% |
| AAATTAAAGCTGCCGTGGGA+TGG | + | chr7_1:7070345-7070364 | Msa1007530:intron | 45.0% | |
| !! | TACAAAATAAAAATAAAAAA+TGG | - | chr7_1:7068155-7068174 | None:intergenic | 5.0% |
| !!! | GTAAAATAAAAATTTATTTT+GGG | - | chr7_1:7069036-7069055 | None:intergenic | 5.0% |
| !!! | CAAAATAAATTTTTATTTTA+CGG | + | chr7_1:7069035-7069054 | Msa1007530:intron | 5.0% |
| !!! | TTTTTATAATAAAATTTACT+TGG | - | chr7_1:7069241-7069260 | None:intergenic | 5.0% |
| !!! | ATTTTAAATTAAATTTTTTG+AGG | + | chr7_1:7070044-7070063 | Msa1007530:intron | 5.0% |
| AGCCTCTTCTGCTAATCGAC+GGG | - | chr7_1:7068522-7068541 | None:intergenic | 50.0% | |
| GAGCCTCTTCTGCTAATCGA+CGG | - | chr7_1:7068523-7068542 | None:intergenic | 50.0% | |
| ! | AGCCCGTCGATTAGCAAAAG+AGG | + | chr7_1:7068571-7068590 | Msa1007530:CDS | 50.0% |
| ! | TGCTGCCTCTTTTGCTTTGC+GGG | - | chr7_1:7068621-7068640 | None:intergenic | 50.0% |
| ! | CTGCTGCCTCTTTTGCTTTG+CGG | - | chr7_1:7068622-7068641 | None:intergenic | 50.0% |
| TCGTGTGTGGGCAGTAAAGA+CGG | - | chr7_1:7068694-7068713 | None:intergenic | 50.0% | |
| !! | TTGGAGGAGTACTCTTCAGC+TGG | - | chr7_1:7069313-7069332 | None:intergenic | 50.0% |
| ACAACGTACCATCCCCAACA+TGG | + | chr7_1:7069631-7069650 | Msa1007530:intron | 50.0% | |
| AGTGAACGTGCACCATGTTG+GGG | - | chr7_1:7069646-7069665 | None:intergenic | 50.0% | |
| GAGTGAACGTGCACCATGTT+GGG | - | chr7_1:7069647-7069666 | None:intergenic | 50.0% | |
| AGAGTGAACGTGCACCATGT+TGG | - | chr7_1:7069648-7069667 | None:intergenic | 50.0% | |
| CTCTACAGAGGAGATCCACA+TGG | + | chr7_1:7069664-7069683 | Msa1007530:intron | 50.0% | |
| !! | TGAGAGGGTGTGATAGTGGA+CGG | - | chr7_1:7070121-7070140 | None:intergenic | 50.0% |
| ! | GTTGTGAGAGGGTGTGATAG+TGG | - | chr7_1:7070125-7070144 | None:intergenic | 50.0% |
| TCACACCCTCTCACAACAAG+CGG | + | chr7_1:7070128-7070147 | Msa1007530:intron | 50.0% | |
| GCAACGAAAATGCCATCCCA+CGG | - | chr7_1:7070360-7070379 | None:intergenic | 50.0% | |
| ! | AGCCCGTCGATTAGCAGAAG+AGG | + | chr7_1:7068517-7068536 | Msa1007530:CDS | 55.0% |
| ACAGGCCCGCAAAGCAAAAG+AGG | + | chr7_1:7068613-7068632 | Msa1007530:CDS | 55.0% | |
| ACGGTGCATTGCATCAGCAG+TGG | - | chr7_1:7068675-7068694 | None:intergenic | 55.0% | |
| AACGTGCACCATGTTGGGGA+TGG | - | chr7_1:7069642-7069661 | None:intergenic | 55.0% | |
| GGTGCACGTTCACTCTACAG+AGG | + | chr7_1:7069652-7069671 | Msa1007530:intron | 55.0% | |
| TCGTCACTAGAGTCGGCCCA+AGG | + | chr7_1:7069830-7069849 | Msa1007530:intron | 60.0% | |
| !! | GAGGGTGTGATAGTGGACGG+CGG | - | chr7_1:7070118-7070137 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 7067999 | 7070586 | 7067999 | ID=Msa1007530;Name=Msa1007530 |
| chr7_1 | mRNA | 7067999 | 7070586 | 7067999 | ID=Msa1007530-mRNA-1;Parent=Msa1007530;Name=Msa1007530-mRNA-1;_AED=0.57;_eAED=0.57;_QI=0|0|0|0.25|1|1|4|0|147 |
| chr7_1 | exon | 7067999 | 7068059 | 7067999 | ID=Msa1007530-mRNA-1:exon:2137;Parent=Msa1007530-mRNA-1 |
| chr7_1 | exon | 7068176 | 7068219 | 7068176 | ID=Msa1007530-mRNA-1:exon:2138;Parent=Msa1007530-mRNA-1 |
| chr7_1 | exon | 7068473 | 7068760 | 7068473 | ID=Msa1007530-mRNA-1:exon:2139;Parent=Msa1007530-mRNA-1 |
| chr7_1 | exon | 7070536 | 7070586 | 7070536 | ID=Msa1007530-mRNA-1:exon:2140;Parent=Msa1007530-mRNA-1 |
| chr7_1 | CDS | 7067999 | 7068059 | 7067999 | ID=Msa1007530-mRNA-1:cds;Parent=Msa1007530-mRNA-1 |
| chr7_1 | CDS | 7068176 | 7068219 | 7068176 | ID=Msa1007530-mRNA-1:cds;Parent=Msa1007530-mRNA-1 |
| chr7_1 | CDS | 7068473 | 7068760 | 7068473 | ID=Msa1007530-mRNA-1:cds;Parent=Msa1007530-mRNA-1 |
| chr7_1 | CDS | 7070536 | 7070586 | 7070536 | ID=Msa1007530-mRNA-1:cds;Parent=Msa1007530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
