AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1034660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1034660 MtrunA17_Chr7g0257681 97.388 268 7 0 1 268 1 268 0.0 540
Msa1034660 MtrunA17_Chr1g0173421 67.596 287 72 4 1 268 1 285 2.02e-133 378
Msa1034660 MtrunA17_Chr6g0451591 42.675 157 82 3 2 154 50 202 3.12e-36 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1034660 AT2G41835.1 58.803 284 94 5 1 268 1 277 4.56e-112 324
Msa1034660 AT3G57480.2 58.209 268 91 4 1 268 1 247 1.45e-103 301
Msa1034660 AT3G57480.1 58.209 268 91 4 1 268 1 247 1.45e-103 301
Msa1034660 AT3G28210.2 44.872 156 77 4 2 154 5 154 7.15e-36 127
Msa1034660 AT3G28210.1 44.872 156 77 4 2 154 5 154 7.15e-36 127
Msa1034660 AT5G48205.1 55.046 109 21 3 1 106 1 84 9.01e-29 108

Find 104 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCCCATTCCTGGATTTC+TGG -0.027304 7_1:-70202196 Msa1034660:five_prime_UTR
GTTCATTAAATAATTTATTT+TGG 0.197602 7_1:+70202276 None:intergenic
TGACGTGGTTGAAGTTAAAT+TGG 0.210618 7_1:+70200322 None:intergenic
AATTGTTAGAGCGTGGAATT+AGG 0.227594 7_1:-70202231 Msa1034660:five_prime_UTR
TTGGATCTTGGTCTGGAATT+AGG 0.236801 7_1:+70200609 None:intergenic
TAATTAGAGAATCATAATTT+TGG 0.240465 7_1:+70202254 None:intergenic
AGGAGTGGCGGCAACCAATA+TGG 0.258450 7_1:-70200134 Msa1034660:CDS
CTACAACTCATCGTTGTCTT+TGG 0.272183 7_1:-70202310 Msa1034660:five_prime_UTR
AATGAATAGCACTTTCTTGA+TGG 0.275542 7_1:-70199982 Msa1034660:three_prime_UTR
TCCTGGATTTCTGGTTGGTT+GGG 0.282507 7_1:-70202187 Msa1034660:five_prime_UTR
ACCAAGATCCAAACATAGTT+TGG 0.297216 7_1:-70200598 Msa1034660:CDS
AGACGATTCCCTCCCATTCC+TGG 0.309154 7_1:-70202204 Msa1034660:five_prime_UTR
ACATGCTCTACAAGGGAAAC+TGG 0.328561 7_1:+70200056 None:intergenic
AGGATTTCGTTTCTTCTTTA+CGG 0.334683 7_1:-70202066 Msa1034660:five_prime_UTR
CCCAAACTATGTTTGGATCT+TGG 0.346442 7_1:+70200597 None:intergenic
GACTTCAAGCCTTCTTGACT+TGG 0.353989 7_1:-70200291 Msa1034660:CDS
TTCCTGGATTTCTGGTTGGT+TGG 0.357981 7_1:-70202188 Msa1034660:five_prime_UTR
TTGCCTAGATCTGGGAATTC+AGG 0.358612 7_1:+70202020 None:intergenic
CACTTGTGATCGTTGCAATC+AGG 0.365381 7_1:-70201952 Msa1034660:intron
GAACAATGCTTGCCTAGATC+TGG 0.368668 7_1:+70202011 None:intergenic
TTCATACATACACCGATATA+CGG 0.368726 7_1:-70199821 Msa1034660:three_prime_UTR
CCCATTCCTGGATTTCTGGT+TGG 0.369171 7_1:-70202192 Msa1034660:five_prime_UTR
AACGGTGACATCTTGTTTGT+TGG 0.370225 7_1:+70200664 None:intergenic
TATGTCCACTTTGTGCTAAA+GGG 0.378295 7_1:-70200637 Msa1034660:CDS
CAGAAAACCTTTACTGCACT+TGG 0.385251 7_1:+70200082 None:intergenic
GGTCCAGGACATTTGTGATC+AGG 0.386396 7_1:+70200416 None:intergenic
ACACCTTCTTTGCCCCATAT+TGG 0.386967 7_1:+70200120 None:intergenic
TTGTGAATTACGCGGATATA+GGG 0.387535 7_1:+70202148 None:intergenic
GGAGTGGCGGCAACCAATAT+GGG 0.400426 7_1:-70200133 Msa1034660:CDS
TGACGCTCCCAAACTATGTT+TGG 0.404477 7_1:+70200590 None:intergenic
CATTGTTTGAAGCACAGGTT+TGG 0.404986 7_1:-70200440 Msa1034660:CDS
ACTAAGGTCTCTCTGCATCC+TGG 0.410212 7_1:+70200503 None:intergenic
ATATGTCCACTTTGTGCTAA+AGG 0.412254 7_1:-70200638 Msa1034660:CDS
TTGACCATTGTTTGAAGCAC+AGG 0.415680 7_1:-70200445 Msa1034660:CDS
TCATCGTTGTCTTTGGCAAA+TGG 0.418030 7_1:-70202303 Msa1034660:five_prime_UTR
ATATTAGATTTATTTGATCA+TGG 0.419688 7_1:-70199887 Msa1034660:three_prime_UTR
AGAAGAATCAATCCGTATAT+CGG 0.427532 7_1:+70199809 None:intergenic
TCGGTGTATGTATGAATTGT+AGG 0.428418 7_1:+70199828 None:intergenic
CTAAGGTCTCTCTGCATCCT+GGG 0.434135 7_1:+70200504 None:intergenic
GACGTGGTTGAAGTTAAATT+GGG 0.435317 7_1:+70200323 None:intergenic
ACTATGTTTGGATCTTGGTC+TGG 0.435501 7_1:+70200602 None:intergenic
ATTGTTAGAGCGTGGAATTA+GGG 0.437851 7_1:-70202230 Msa1034660:five_prime_UTR
ACGGTGACATCTTGTTTGTT+GGG 0.438220 7_1:+70200665 None:intergenic
CCCTCTCAACATGCTCTACA+AGG 0.443451 7_1:+70200048 None:intergenic
ATTGTGAATTACGCGGATAT+AGG 0.451113 7_1:+70202147 None:intergenic
ACGGTTTCAGCTTGGATCAT+GGG 0.451301 7_1:-70202047 Msa1034660:exon
TACGGTTTCAGCTTGGATCA+TGG 0.451686 7_1:-70202048 Msa1034660:exon
CCAAGATCCAAACATAGTTT+GGG 0.458482 7_1:-70200597 Msa1034660:CDS
GTCTCTCTGCATCCTGGGGC+AGG 0.461132 7_1:+70200509 None:intergenic
CTTCTTTGTTGTAATGCTGT+TGG 0.468468 7_1:-70199791 Msa1034660:three_prime_UTR
TCTTCTTTACGGTTTCAGCT+TGG 0.473269 7_1:-70202055 Msa1034660:five_prime_UTR
TGGTACTAGTAATAATCAAC+TGG 0.476635 7_1:-70200231 Msa1034660:CDS
GATTGTGTTTGAGAATACTA+AGG 0.477004 7_1:+70200487 None:intergenic
GCATGCCCCAAGTGCAGTAA+AGG 0.479086 7_1:-70200089 Msa1034660:CDS
TATTAGATTTATTTGATCAT+GGG 0.481987 7_1:-70199886 Msa1034660:three_prime_UTR
GCTTCTAAATTGAGTGGGAT+AGG 0.483203 7_1:-70200269 Msa1034660:CDS
ACTCCTGAATTCCCAGATCT+AGG 0.493436 7_1:-70202023 Msa1034660:CDS
TCCCAACCAACCAGAAATCC+AGG 0.504931 7_1:+70202186 None:intergenic
ATCCACCAAACTCGTGGCCG+AGG 0.511045 7_1:+70200178 None:intergenic
CAACCAATATGGGGCAAAGA+AGG 0.517022 7_1:-70200123 Msa1034660:CDS
TTCCTCGGCCACGAGTTTGG+TGG 0.518637 7_1:-70200180 Msa1034660:CDS
CTCAAACACAATCAAGTGTA+GGG 0.519840 7_1:-70200477 Msa1034660:CDS
ATTTAGAAGCCAAGTCAAGA+AGG 0.521688 7_1:+70200282 None:intergenic
CCAACCAGAAATCCAGGAAT+GGG 0.522636 7_1:+70202192 None:intergenic
GGACCTGATCACAAATGTCC+TGG 0.523810 7_1:-70200419 Msa1034660:CDS
AGAAAACCTTTACTGCACTT+GGG 0.532761 7_1:+70200083 None:intergenic
CATGCTCTACAAGGGAAACT+GGG 0.533711 7_1:+70200057 None:intergenic
ACTTGGCTTCTAAATTGAGT+GGG 0.535363 7_1:-70200274 Msa1034660:CDS
AACAATGCTTGCCTAGATCT+GGG 0.537422 7_1:+70202012 None:intergenic
CTAATTAAATTGTTAGAGCG+TGG 0.540106 7_1:-70202238 Msa1034660:five_prime_UTR
CTTCAACCACGTCATCTAAG+TGG 0.540578 7_1:-70200313 Msa1034660:CDS
CCTTGTAGAGCATGTTGAGA+GGG 0.543468 7_1:-70200048 Msa1034660:CDS
ACCAACCAGAAATCCAGGAA+TGG 0.544610 7_1:+70202191 None:intergenic
GTTGTTCTTGAGAATTGTTG+TGG 0.549442 7_1:-70200016 Msa1034660:exon
GACTTGGCTTCTAAATTGAG+TGG 0.550800 7_1:-70200275 Msa1034660:CDS
CGGTTTCAGCTTGGATCATG+GGG 0.551498 7_1:-70202046 Msa1034660:exon
GTTAGAGCGTGGAATTAGGG+AGG 0.551696 7_1:-70202227 Msa1034660:five_prime_UTR
CAAACCTGTGCTTCAAACAA+TGG 0.566927 7_1:+70200441 None:intergenic
GAGCATGTTGAGAGGGATCA+TGG 0.569049 7_1:-70200041 Msa1034660:CDS
ATGTCCACTTTGTGCTAAAG+GGG 0.570588 7_1:-70200636 Msa1034660:CDS
GAAAACCTTTACTGCACTTG+GGG 0.571579 7_1:+70200084 None:intergenic
TCTCAAACACAATCAAGTGT+AGG 0.571728 7_1:-70200478 Msa1034660:CDS
ATAGGACAGAGCAGTGGCAG+TGG 0.577401 7_1:-70200251 Msa1034660:CDS
ACCAGAAATCCAGGAATGGG+AGG 0.581773 7_1:+70202195 None:intergenic
CGCATGATCCACCAAACTCG+TGG 0.584791 7_1:+70200172 None:intergenic
ATTGCAACGATCACAAGTGA+AGG 0.586850 7_1:+70201956 None:intergenic
AGTCTTTGGAATATGAGTAA+TGG 0.594698 7_1:-70200362 Msa1034660:CDS
AATTATGCTCAATATAACTT+CGG 0.599455 7_1:+70200696 None:intergenic
CCCTTGTAGAGCATGTTGAG+AGG 0.611040 7_1:-70200049 Msa1034660:CDS
GAAAACTTAGCATCACATAG+TGG 0.613925 7_1:+70200200 None:intergenic
CGAACCCCTTTAGCACAAAG+TGG 0.621156 7_1:+70200632 None:intergenic
CCAGAAATCCAGGAATGGGA+GGG 0.629567 7_1:+70202196 None:intergenic
AAAGTTCATCAAAGGAGTGG+CGG 0.638449 7_1:-70200146 Msa1034660:CDS
TGAAGTCCACTTAGATGACG+TGG 0.643218 7_1:+70200307 None:intergenic
AGAAACGAAATCCTTCCAGA+AGG 0.643633 7_1:+70202075 None:intergenic
TTGCAACGATCACAAGTGAA+GGG 0.653221 7_1:+70201957 None:intergenic
CCTCTCAACATGCTCTACAA+GGG 0.654661 7_1:+70200049 None:intergenic
TAAGGTCTCTCTGCATCCTG+GGG 0.659577 7_1:+70200505 None:intergenic
GAGTGGCGGCAACCAATATG+GGG 0.665147 7_1:-70200132 Msa1034660:CDS
AATCAAGTGTAGGGACTGTG+AGG 0.678787 7_1:-70200468 Msa1034660:CDS
AGTGGGATAGGACAGAGCAG+TGG 0.703021 7_1:-70200257 Msa1034660:CDS
GTGGAGAATTGTGAATTACG+CGG 0.729104 7_1:+70202140 None:intergenic
ACAAAGTGGACATATAACAA+CGG 0.739755 7_1:+70200646 None:intergenic
CATGTTGAGAGGGATCATGG+TGG 0.808965 7_1:-70200038 Msa1034660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTCATTAAATAATTTATTT+TGG + chr7_1:70199914-70199933 None:intergenic 10.0%
!! AAAAGAAAAAAAAAAAATAG+TGG + chr7_1:70200069-70200088 None:intergenic 10.0%
!!! AAATTTTTTTCAAAAAGAAA+TGG - chr7_1:70200801-70200820 Msa1034660:intron 10.0%
!!! GTTATTTTCATAAATTATTT+CGG - chr7_1:70201194-70201213 Msa1034660:intron 10.0%
!!! TAATTAGAGAATCATAATTT+TGG + chr7_1:70199936-70199955 None:intergenic 15.0%
!! TTGTTTCGAAATTAATTTAT+TGG - chr7_1:70200292-70200311 Msa1034660:CDS 15.0%
!! AGAAAATAATAAAATGAAAC+TGG + chr7_1:70200342-70200361 None:intergenic 15.0%
!!! ATTTAAAGTAAATCATTTAC+TGG - chr7_1:70200941-70200960 Msa1034660:intron 15.0%
!! ATATTAGATTTATTTGATCA+TGG - chr7_1:70202300-70202319 Msa1034660:five_prime_UTR 15.0%
!! TATTAGATTTATTTGATCAT+GGG - chr7_1:70202301-70202320 Msa1034660:five_prime_UTR 15.0%
!!! AAGCAATGTTTTTAATCAAA+AGG + chr7_1:70200393-70200412 None:intergenic 20.0%
!! GATTGTATATATACTACTAT+TGG - chr7_1:70200743-70200762 Msa1034660:intron 20.0%
!!! AAACACAAATTTCTAAAAGT+TGG - chr7_1:70200849-70200868 Msa1034660:intron 20.0%
!!! AACACAAATTTCTAAAAGTT+GGG - chr7_1:70200850-70200869 Msa1034660:intron 20.0%
!!! TATCATCTATATAGAGTATT+CGG - chr7_1:70201069-70201088 Msa1034660:intron 20.0%
!! AATCATTTACATAAACTGTT+CGG + chr7_1:70201262-70201281 None:intergenic 20.0%
!! AATTATGCTCAATATAACTT+CGG + chr7_1:70201494-70201513 None:intergenic 20.0%
!!! CAATATTTTGCTATCAAATA+GGG + chr7_1:70202258-70202277 None:intergenic 20.0%
!!! ACAATATTTTGCTATCAAAT+AGG + chr7_1:70202259-70202278 None:intergenic 20.0%
! CAATATCGATATTGTTAGTT+TGG - chr7_1:70200453-70200472 Msa1034660:CDS 25.0%
!! TTGCTAGTGTTTGATTTATA+GGG - chr7_1:70200542-70200561 Msa1034660:CDS 25.0%
!!! TATGTTTTTTTCTCGTTTTC+AGG + chr7_1:70200634-70200653 None:intergenic 25.0%
!! ATAGAATTGACTTTGAAAGT+TGG - chr7_1:70200721-70200740 Msa1034660:intron 25.0%
! GTTTCACAAAACTATAGTAA+TGG + chr7_1:70200833-70200852 None:intergenic 25.0%
!! TGGTTATTTCATTTGAGAAA+TGG - chr7_1:70200899-70200918 Msa1034660:intron 25.0%
! AAACTATACAATAACCTTAC+TGG + chr7_1:70201050-70201069 None:intergenic 25.0%
! GTAACTTAATTAAGTGCTTA+CGG - chr7_1:70201096-70201115 Msa1034660:intron 25.0%
! TGAATTTATTGAAACAAGCT+AGG - chr7_1:70201217-70201236 Msa1034660:intron 25.0%
!!! AAACTTGTTTCCAACTTTTT+GGG + chr7_1:70201795-70201814 None:intergenic 25.0%
!!! AAAACTTGTTTCCAACTTTT+TGG + chr7_1:70201796-70201815 None:intergenic 25.0%
!! GTTTTTCATTTATGAGTCTT+TGG - chr7_1:70201811-70201830 Msa1034660:intron 25.0%
CTAATTAAATTGTTAGAGCG+TGG - chr7_1:70199949-70199968 Msa1034660:three_prime_UTR 30.0%
AGAAGGAAAAAAATGAGGAA+TGG + chr7_1:70200098-70200117 None:intergenic 30.0%
!!! ATTCCTCATTTTTTTCCTTC+TGG - chr7_1:70200097-70200116 Msa1034660:CDS 30.0%
AGGATTTCGTTTCTTCTTTA+CGG - chr7_1:70200121-70200140 Msa1034660:CDS 30.0%
! GTTGCTAGTGTTTGATTTAT+AGG - chr7_1:70200541-70200560 Msa1034660:CDS 30.0%
! TGCTAGTGTTTGATTTATAG+GGG - chr7_1:70200543-70200562 Msa1034660:CDS 30.0%
ATGAACTTAAGTGATTAAGC+TGG - chr7_1:70200879-70200898 Msa1034660:intron 30.0%
TTACTGGAAATAATGAACTG+AGG - chr7_1:70200957-70200976 Msa1034660:intron 30.0%
GCTTAATAGAAACTTCAATG+AGG + chr7_1:70201008-70201027 None:intergenic 30.0%
TGAGGATGATGTACATATAT+TGG - chr7_1:70201372-70201391 Msa1034660:intron 30.0%
AACAATATACCTGCAAAAGA+TGG + chr7_1:70201464-70201483 None:intergenic 30.0%
! TCTTTTGCAGGTATATTGTT+TGG - chr7_1:70201464-70201483 Msa1034660:intron 30.0%
! CTTTTGCAGGTATATTGTTT+GGG - chr7_1:70201465-70201484 Msa1034660:intron 30.0%
! TTTTGCAGGTATATTGTTTG+GGG - chr7_1:70201466-70201485 Msa1034660:intron 30.0%
ACAAAGTGGACATATAACAA+CGG + chr7_1:70201544-70201563 None:intergenic 30.0%
!!! GTGGCTTTTTCATAATTTGA+TGG + chr7_1:70201642-70201661 None:intergenic 30.0%
GATTGTGTTTGAGAATACTA+AGG + chr7_1:70201703-70201722 None:intergenic 30.0%
!! AGTCTTTGGAATATGAGTAA+TGG - chr7_1:70201825-70201844 Msa1034660:intron 30.0%
TGGTACTAGTAATAATCAAC+TGG - chr7_1:70201956-70201975 Msa1034660:CDS 30.0%
ATGCGAAAAAAGTTCATCAA+AGG - chr7_1:70202033-70202052 Msa1034660:exon 30.0%
AAAAAAGTTCATCAAAGGAG+TGG - chr7_1:70202038-70202057 Msa1034660:exon 30.0%
AATGAATAGCACTTTCTTGA+TGG - chr7_1:70202205-70202224 Msa1034660:five_prime_UTR 30.0%
GCTCACAAATAACGAAAAAT+AGG + chr7_1:70202340-70202359 None:intergenic 30.0%
TTCATACATACACCGATATA+CGG - chr7_1:70202366-70202385 Msa1034660:five_prime_UTR 30.0%
AGAAGAATCAATCCGTATAT+CGG + chr7_1:70202381-70202400 None:intergenic 30.0%
AATTGTTAGAGCGTGGAATT+AGG - chr7_1:70199956-70199975 Msa1034660:three_prime_UTR 35.0%
ATTGTTAGAGCGTGGAATTA+GGG - chr7_1:70199957-70199976 Msa1034660:three_prime_UTR 35.0%
TTGTGAATTACGCGGATATA+GGG + chr7_1:70200042-70200061 None:intergenic 35.0%
ATTGTGAATTACGCGGATAT+AGG + chr7_1:70200043-70200062 None:intergenic 35.0%
CTTCCAGAAGGAAAAAAATG+AGG + chr7_1:70200103-70200122 None:intergenic 35.0%
!!! CTCATTTTTTTCCTTCTGGA+AGG - chr7_1:70200101-70200120 Msa1034660:CDS 35.0%
! AGTGTTTGATTTATAGGGGT+TGG - chr7_1:70200547-70200566 Msa1034660:CDS 35.0%
! GTGTTTGATTTATAGGGGTT+GGG - chr7_1:70200548-70200567 Msa1034660:CDS 35.0%
TGAGCTAAAGGTTAATGAGT+TGG - chr7_1:70200592-70200611 Msa1034660:CDS 35.0%
!!! TTTTGACTTCTCCAGTTCTT+CGG - chr7_1:70201319-70201338 Msa1034660:intron 35.0%
! AATTTTCAAACCCGAAGAAC+TGG + chr7_1:70201333-70201352 None:intergenic 35.0%
GATGTACATATATTGGATCG+TGG - chr7_1:70201379-70201398 Msa1034660:intron 35.0%
GAGTTGCTTCATTGAAAACA+AGG + chr7_1:70201416-70201435 None:intergenic 35.0%
AATGAAGCAACTCTTTGTGT+AGG - chr7_1:70201423-70201442 Msa1034660:intron 35.0%
ATATGTCCACTTTGTGCTAA+AGG - chr7_1:70201549-70201568 Msa1034660:intron 35.0%
TATGTCCACTTTGTGCTAAA+GGG - chr7_1:70201550-70201569 Msa1034660:intron 35.0%
ACCAAGATCCAAACATAGTT+TGG - chr7_1:70201589-70201608 Msa1034660:intron 35.0%
CCAAGATCCAAACATAGTTT+GGG - chr7_1:70201590-70201609 Msa1034660:intron 35.0%
!!! AGGACACTTTTTCTTCTTTG+TGG + chr7_1:70201661-70201680 None:intergenic 35.0%
TCTCAAACACAATCAAGTGT+AGG - chr7_1:70201709-70201728 Msa1034660:intron 35.0%
CTCAAACACAATCAAGTGTA+GGG - chr7_1:70201710-70201729 Msa1034660:intron 35.0%
GACGTGGTTGAAGTTAAATT+GGG + chr7_1:70201867-70201886 None:intergenic 35.0%
TGACGTGGTTGAAGTTAAAT+TGG + chr7_1:70201868-70201887 None:intergenic 35.0%
ATTTAGAAGCCAAGTCAAGA+AGG + chr7_1:70201908-70201927 None:intergenic 35.0%
ACTTGGCTTCTAAATTGAGT+GGG - chr7_1:70201913-70201932 Msa1034660:intron 35.0%
GAAAACTTAGCATCACATAG+TGG + chr7_1:70201990-70202009 None:intergenic 35.0%
! ATGTGATGCTAAGTTTTCCT+CGG - chr7_1:70201992-70202011 Msa1034660:CDS 35.0%
AGAAAACCTTTACTGCACTT+GGG + chr7_1:70202107-70202126 None:intergenic 35.0%
GTTGTTCTTGAGAATTGTTG+TGG - chr7_1:70202171-70202190 Msa1034660:five_prime_UTR 35.0%
TCGGTGTATGTATGAATTGT+AGG + chr7_1:70202362-70202381 None:intergenic 35.0%
! CTTCTTTGTTGTAATGCTGT+TGG - chr7_1:70202396-70202415 Msa1034660:five_prime_UTR 35.0%
CTACAACTCATCGTTGTCTT+TGG - chr7_1:70199877-70199896 Msa1034660:three_prime_UTR 40.0%
TCATCGTTGTCTTTGGCAAA+TGG - chr7_1:70199884-70199903 Msa1034660:three_prime_UTR 40.0%
GTGGAGAATTGTGAATTACG+CGG + chr7_1:70200050-70200069 None:intergenic 40.0%
AGAAACGAAATCCTTCCAGA+AGG + chr7_1:70200115-70200134 None:intergenic 40.0%
TCTTCTTTACGGTTTCAGCT+TGG - chr7_1:70200132-70200151 Msa1034660:CDS 40.0%
AACAATGCTTGCCTAGATCT+GGG + chr7_1:70200178-70200197 None:intergenic 40.0%
TTGCAACGATCACAAGTGAA+GGG + chr7_1:70200233-70200252 None:intergenic 40.0%
ATTGCAACGATCACAAGTGA+AGG + chr7_1:70200234-70200253 None:intergenic 40.0%
GTTTGGATACATCTCGAAGT+TGG - chr7_1:70200470-70200489 Msa1034660:CDS 40.0%
ATTTATAGGGGTTGGGTAGT+GGG - chr7_1:70200555-70200574 Msa1034660:CDS 40.0%
GCTTAATGCAAAAGCCAGTA+AGG - chr7_1:70201033-70201052 Msa1034660:intron 40.0%
! TTTGACTTCTCCAGTTCTTC+GGG - chr7_1:70201320-70201339 Msa1034660:intron 40.0%
! GTTGCTGTGATAGACTTTTG+AGG - chr7_1:70201354-70201373 Msa1034660:intron 40.0%
! ATATCAGCTCCATCTTTTGC+AGG - chr7_1:70201452-70201471 Msa1034660:intron 40.0%
! ACGGTGACATCTTGTTTGTT+GGG + chr7_1:70201525-70201544 None:intergenic 40.0%
! AACGGTGACATCTTGTTTGT+TGG + chr7_1:70201526-70201545 None:intergenic 40.0%
ATGTCCACTTTGTGCTAAAG+GGG - chr7_1:70201551-70201570 Msa1034660:intron 40.0%
! TTGGATCTTGGTCTGGAATT+AGG + chr7_1:70201581-70201600 None:intergenic 40.0%
ACTATGTTTGGATCTTGGTC+TGG + chr7_1:70201588-70201607 None:intergenic 40.0%
CCCAAACTATGTTTGGATCT+TGG + chr7_1:70201593-70201612 None:intergenic 40.0%
! TTGACCATTGTTTGAAGCAC+AGG - chr7_1:70201742-70201761 Msa1034660:intron 40.0%
CAAACCTGTGCTTCAAACAA+TGG + chr7_1:70201749-70201768 None:intergenic 40.0%
! CATTGTTTGAAGCACAGGTT+TGG - chr7_1:70201747-70201766 Msa1034660:intron 40.0%
GACTTGGCTTCTAAATTGAG+TGG - chr7_1:70201912-70201931 Msa1034660:intron 40.0%
GCTTCTAAATTGAGTGGGAT+AGG - chr7_1:70201918-70201937 Msa1034660:intron 40.0%
AAAGTTCATCAAAGGAGTGG+CGG - chr7_1:70202041-70202060 Msa1034660:exon 40.0%
GAAAACCTTTACTGCACTTG+GGG + chr7_1:70202106-70202125 None:intergenic 40.0%
CAGAAAACCTTTACTGCACT+TGG + chr7_1:70202108-70202127 None:intergenic 40.0%
! CAAGTGCAGTAAAGGTTTTC+TGG - chr7_1:70202106-70202125 Msa1034660:five_prime_UTR 40.0%
CCAACCAGAAATCCAGGAAT+GGG + chr7_1:70199998-70200017 None:intergenic 45.0%
ACCAACCAGAAATCCAGGAA+TGG + chr7_1:70199999-70200018 None:intergenic 45.0%
!! TTCCTGGATTTCTGGTTGGT+TGG - chr7_1:70199999-70200018 Msa1034660:three_prime_UTR 45.0%
!! TCCTGGATTTCTGGTTGGTT+GGG - chr7_1:70200000-70200019 Msa1034660:three_prime_UTR 45.0%
TACGGTTTCAGCTTGGATCA+TGG - chr7_1:70200139-70200158 Msa1034660:CDS 45.0%
ACGGTTTCAGCTTGGATCAT+GGG - chr7_1:70200140-70200159 Msa1034660:CDS 45.0%
ACTCCTGAATTCCCAGATCT+AGG - chr7_1:70200164-70200183 Msa1034660:CDS 45.0%
TTGCCTAGATCTGGGAATTC+AGG + chr7_1:70200170-70200189 None:intergenic 45.0%
GAACAATGCTTGCCTAGATC+TGG + chr7_1:70200179-70200198 None:intergenic 45.0%
CACTTGTGATCGTTGCAATC+AGG - chr7_1:70200235-70200254 Msa1034660:CDS 45.0%
!! CATCTCGAAGTTGGTTTGTG+TGG - chr7_1:70200479-70200498 Msa1034660:CDS 45.0%
GATTTATAGGGGTTGGGTAG+TGG - chr7_1:70200554-70200573 Msa1034660:CDS 45.0%
! GCTCAACTAGTCTGAGCTAA+AGG - chr7_1:70200580-70200599 Msa1034660:CDS 45.0%
! GGTTAATGAGTTGGAAGTCC+AGG - chr7_1:70200601-70200620 Msa1034660:CDS 45.0%
!! TCGTTTTCAGGACTTGAACC+TGG + chr7_1:70200622-70200641 None:intergenic 45.0%
CTGTTCGGCTCATGTTCATA+GGG + chr7_1:70201247-70201266 None:intergenic 45.0%
ACTGTTCGGCTCATGTTCAT+AGG + chr7_1:70201248-70201267 None:intergenic 45.0%
TGACGCTCCCAAACTATGTT+TGG + chr7_1:70201600-70201619 None:intergenic 45.0%
AATCAAGTGTAGGGACTGTG+AGG - chr7_1:70201719-70201738 Msa1034660:intron 45.0%
ATGTCCTGGACCCAAAAAGT+TGG - chr7_1:70201782-70201801 Msa1034660:intron 45.0%
!!! GTTTCCAACTTTTTGGGTCC+AGG + chr7_1:70201789-70201808 None:intergenic 45.0%
CTTCAACCACGTCATCTAAG+TGG - chr7_1:70201874-70201893 Msa1034660:intron 45.0%
TGAAGTCCACTTAGATGACG+TGG + chr7_1:70201883-70201902 None:intergenic 45.0%
GACTTCAAGCCTTCTTGACT+TGG - chr7_1:70201896-70201915 Msa1034660:intron 45.0%
CAACCAATATGGGGCAAAGA+AGG - chr7_1:70202064-70202083 Msa1034660:five_prime_UTR 45.0%
ACACCTTCTTTGCCCCATAT+TGG + chr7_1:70202070-70202089 None:intergenic 45.0%
CATGCTCTACAAGGGAAACT+GGG + chr7_1:70202133-70202152 None:intergenic 45.0%
ACATGCTCTACAAGGGAAAC+TGG + chr7_1:70202134-70202153 None:intergenic 45.0%
CCTCTCAACATGCTCTACAA+GGG + chr7_1:70202141-70202160 None:intergenic 45.0%
CCTTGTAGAGCATGTTGAGA+GGG - chr7_1:70202139-70202158 Msa1034660:five_prime_UTR 45.0%
GTTAGAGCGTGGAATTAGGG+AGG - chr7_1:70199960-70199979 Msa1034660:three_prime_UTR 50.0%
CCAGAAATCCAGGAATGGGA+GGG + chr7_1:70199994-70200013 None:intergenic 50.0%
ACCAGAAATCCAGGAATGGG+AGG + chr7_1:70199995-70200014 None:intergenic 50.0%
!! CCCATTCCTGGATTTCTGGT+TGG - chr7_1:70199995-70200014 Msa1034660:three_prime_UTR 50.0%
TCCCAACCAACCAGAAATCC+AGG + chr7_1:70200004-70200023 None:intergenic 50.0%
CGGTTTCAGCTTGGATCATG+GGG - chr7_1:70200141-70200160 Msa1034660:CDS 50.0%
CGAACCCCTTTAGCACAAAG+TGG + chr7_1:70201558-70201577 None:intergenic 50.0%
!! AAGAAAAAGTGTCCTGCCCC+AGG - chr7_1:70201666-70201685 Msa1034660:intron 50.0%
TAAGGTCTCTCTGCATCCTG+GGG + chr7_1:70201685-70201704 None:intergenic 50.0%
CTAAGGTCTCTCTGCATCCT+GGG + chr7_1:70201686-70201705 None:intergenic 50.0%
ACTAAGGTCTCTCTGCATCC+TGG + chr7_1:70201687-70201706 None:intergenic 50.0%
GGACCTGATCACAAATGTCC+TGG - chr7_1:70201768-70201787 Msa1034660:intron 50.0%
GGTCCAGGACATTTGTGATC+AGG + chr7_1:70201774-70201793 None:intergenic 50.0%
CCCTTGTAGAGCATGTTGAG+AGG - chr7_1:70202138-70202157 Msa1034660:five_prime_UTR 50.0%
CCCTCTCAACATGCTCTACA+AGG + chr7_1:70202142-70202161 None:intergenic 50.0%
GAGCATGTTGAGAGGGATCA+TGG - chr7_1:70202146-70202165 Msa1034660:five_prime_UTR 50.0%
CATGTTGAGAGGGATCATGG+TGG - chr7_1:70202149-70202168 Msa1034660:five_prime_UTR 50.0%
AGACGATTCCCTCCCATTCC+TGG - chr7_1:70199983-70200002 Msa1034660:three_prime_UTR 55.0%
CCCTCCCATTCCTGGATTTC+TGG - chr7_1:70199991-70200010 Msa1034660:three_prime_UTR 55.0%
AGTGGGATAGGACAGAGCAG+TGG - chr7_1:70201930-70201949 Msa1034660:intron 55.0%
ATAGGACAGAGCAGTGGCAG+TGG - chr7_1:70201936-70201955 Msa1034660:intron 55.0%
! GTTTTCCTCGGCCACGAGTT+TGG - chr7_1:70202004-70202023 Msa1034660:CDS 55.0%
CGCATGATCCACCAAACTCG+TGG + chr7_1:70202018-70202037 None:intergenic 55.0%
AGGAGTGGCGGCAACCAATA+TGG - chr7_1:70202053-70202072 Msa1034660:five_prime_UTR 55.0%
GGAGTGGCGGCAACCAATAT+GGG - chr7_1:70202054-70202073 Msa1034660:five_prime_UTR 55.0%
GAGTGGCGGCAACCAATATG+GGG - chr7_1:70202055-70202074 Msa1034660:five_prime_UTR 55.0%
GCATGCCCCAAGTGCAGTAA+AGG - chr7_1:70202098-70202117 Msa1034660:five_prime_UTR 55.0%
TTCCTCGGCCACGAGTTTGG+TGG - chr7_1:70202007-70202026 Msa1034660:CDS 60.0%
ATCCACCAAACTCGTGGCCG+AGG + chr7_1:70202012-70202031 None:intergenic 60.0%
GTCTCTCTGCATCCTGGGGC+AGG + chr7_1:70201681-70201700 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 70199789 70202420 70199789 ID=Msa1034660;Name=Msa1034660
chr7_1 mRNA 70199789 70202420 70199789 ID=Msa1034660-mRNA-1;Parent=Msa1034660;Name=Msa1034660-mRNA-1;_AED=0.03;_eAED=0.03;_QI=369|1|1|1|1|1|2|239|268
chr7_1 exon 70199789 70200735 70199789 ID=Msa1034660-mRNA-1:exon:16751;Parent=Msa1034660-mRNA-1
chr7_1 exon 70201953 70202420 70201953 ID=Msa1034660-mRNA-1:exon:16750;Parent=Msa1034660-mRNA-1
chr7_1 five_prime_UTR 70202052 70202420 70202052 ID=Msa1034660-mRNA-1:five_prime_utr;Parent=Msa1034660-mRNA-1
chr7_1 CDS 70201953 70202051 70201953 ID=Msa1034660-mRNA-1:cds;Parent=Msa1034660-mRNA-1
chr7_1 CDS 70200028 70200735 70200028 ID=Msa1034660-mRNA-1:cds;Parent=Msa1034660-mRNA-1
chr7_1 three_prime_UTR 70199789 70200027 70199789 ID=Msa1034660-mRNA-1:three_prime_utr;Parent=Msa1034660-mRNA-1
Gene Sequence

>Msa1034660

ATGGGGACTCCTGAATTCCCAGATCTAGGCAAGCATTGTTCTGTTTCTGATTGCAGACTCATCGATTTCTTGCCCTTCACTTGTGATCGTTGCAATCAGGTATATTGTTTGGGGCACCGAAGTTATATTGAGCATAATTGTACAAAACCCAACAAACAAGATGTCACCGTTGTTATATGTCCACTTTGTGCTAAAGGGGTTCGCCTAATTCCAGACCAAGATCCAAACATAGTTTGGGAGCGTCATGTGAATACAGACTGTGATCCATCAAATTATGAAAAAGCCACAAAGAAGAAAAAGTGTCCTGCCCCAGGATGCAGAGAGACCTTAGTATTCTCAAACACAATCAAGTGTAGGGACTGTGAGGTTGACCATTGTTTGAAGCACAGGTTTGGACCTGATCACAAATGTCCTGGACCCAAAAAGTTGGAAACAAGTTTTTCATTTATGAGTCTTTGGAATATGAGTAATGGTAAAGAAGAGTCAAAACCCAATTTAACTTCAACCACGTCATCTAAGTGGACTTCAAGCCTTCTTGACTTGGCTTCTAAATTGAGTGGGATAGGACAGAGCAGTGGCAGTGGTACTAGTAATAATCAACTGGAGAAGTGTCCACTATGTGATGCTAAGTTTTCCTCGGCCACGAGTTTGGTGGATCATGCGAAAAAAGTTCATCAAAGGAGTGGCGGCAACCAATATGGGGCAAAGAAGGTGTCGATTTCTGCATGCCCCAAGTGCAGTAAAGGTTTTCTGGACCCAGTTTCCCTTGTAGAGCATGTTGAGAGGGATCATGGTGGTTGTTCTTGA
Protein sequence

>Msa1034660

MGTPEFPDLGKHCSVSDCRLIDFLPFTCDRCNQVYCLGHRSYIEHNCTKPNKQDVTVVICPLCAKGVRLIPDQDPNIVWERHVNTDCDPSNYEKATKKKKCPAPGCRETLVFSNTIKCRDCEVDHCLKHRFGPDHKCPGPKKLETSFSFMSLWNMSNGKEESKPNLTSTTSSKWTSSLLDLASKLSGIGQSSGSGTSNNQLEKCPLCDAKFSSATSLVDHAKKVHQRSGGNQYGAKKVSISACPKCSKGFLDPVSLVEHVERDHGGCS*