AlfalfaGEDB | Msa1066050

M. sativa cultivar zhongmu-4 / Msa1066050

KEGG: K00924|K03083
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009741 GO:0009987 GO:0010033 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0033993 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050896 GO:0071704 GO:0140096 GO:1901564 GO:1901700
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066050	XP_003623822.1	98.938	471	4	1	1	471	1	470	0.0	975

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066050	sp\|Q96287\|KSG8_ARATH	75.532	470	111	3	1	470	1	466	0.0	750

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066050	G7L1H1	98.938	471	4	1	1	471	1	470	0.0	975

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1027590	Msa1066050	0.946852	2.090019e-105	-8.615850e-47
Msa1066050	Msa1155070	0.894321	2.752227e-75	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066050	MtrunA17_Chr7g0246591	98.938	471	4	1	1	471	1	470	0.0	975
Msa1066050	MtrunA17_Chr8g0350311	81.144	472	84	4	1	471	1	468	0.0	811
Msa1066050	MtrunA17_Chr1g0193081	77.461	386	80	1	85	470	25	403	0.0	644
Msa1066050	MtrunA17_Chr7g0270371	73.956	407	92	3	65	470	10	403	0.0	644
Msa1066050	MtrunA17_Chr1g0194201	73.750	400	98	1	71	470	11	403	0.0	634
Msa1066050	MtrunA17_Chr2g0320011	73.784	370	96	1	101	470	47	415	0.0	608
Msa1066050	MtrunA17_Chr2g0277011	73.901	364	95	0	107	470	55	418	0.0	599
Msa1066050	MtrunA17_Chr2g0284091	72.329	365	100	1	106	470	4	367	0.0	596
Msa1066050	MtrunA17_Chr4g0045841	35.616	292	173	7	148	431	61	345	2.21e-55	189
Msa1066050	MtrunA17_Chr4g0030151	34.603	315	188	9	148	453	45	350	5.00e-53	182

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1066050	AT4G00720.1	75.532	470	111	3	1	470	1	466	750
Msa1066050	AT5G14640.1	77.979	386	78	1	85	470	24	402	652
Msa1066050	AT5G14640.2	77.979	386	78	1	85	470	24	402	652
Msa1066050	AT5G26751.1	76.982	391	83	1	80	470	14	397	651
Msa1066050	AT3G05840.2	77.461	386	80	1	85	470	23	401	650
Msa1066050	AT3G05840.1	77.461	386	80	1	85	470	23	401	650
Msa1066050	AT3G61160.2	67.021	470	122	4	1	470	1	437	647
Msa1066050	AT3G61160.1	66.383	470	118	4	1	470	1	430	638
Msa1066050	AT1G57870.1	72.589	394	107	1	77	470	18	410	617
Msa1066050	AT1G57870.4	72.589	394	107	1	77	470	18	410	617

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Find 126 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCACTGTTTCTACTCGA+CGG	0.646352	7_2:-59996303	Msa1066050:CDS
AACGGAAATGGAACGGAAAC+TGG	0.361591	7_2:-59996904	Msa1066050:CDS
AACTACTAACCTTGTGCCAT+GGG	0.484175	7_2:+59995185	None:intergenic
AACTTAAATTCACTGTAATT+TGG	0.156371	7_2:+59995226	None:intergenic
AAGAAACAAACCGTGTTGAG+AGG	0.508397	7_2:-59997212	Msa1066050:CDS
AAGCAGTGGATCTTGTGTCG+AGG	0.709078	7_2:-59995055	Msa1066050:CDS
AAGTTTGATTTAGAAACTGA+AGG	0.565858	7_2:-59997246	Msa1066050:CDS
AATCAACTCCTTGCATGCTC+AGG	0.445757	7_2:+59994652	None:intergenic
AATTATGTACTATAGATCTT+GGG	0.521137	7_2:-59995283	Msa1066050:intron
AATTATTTGCATCATGTGGT+TGG	0.484000	7_2:-59996052	Msa1066050:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTCCCATAGACAAATTA+AGG	+	chr7_2:59994747-59994766	None:intergenic	30.0%
AATTATGGCAAGTGCTAAAT+AGG	-	chr7_2:59995131-59995150	Msa1066050:intron	30.0%
ATTATGGCAAGTGCTAAATA+GGG	-	chr7_2:59995132-59995151	Msa1066050:intron	30.0%
TGATTTAGAAACTGAAGGAA+AGG	-	chr7_2:59995309-59995328	Msa1066050:intron	30.0%
GTAATTACAACTTCTATTGG+TGG	-	chr7_2:59995673-59995692	Msa1066050:CDS	30.0%
GGAGAATTGTATAGCAATTA+AGG	+	chr7_2:59995739-59995758	None:intergenic	30.0%
TTCATACAACAGTGAAACAA+AGG	+	chr7_2:59996057-59996076	None:intergenic	30.0%
CAAACTCCAAAACTAAGTTA+AGG	+	chr7_2:59996292-59996311	None:intergenic	30.0%
CATAAATTATAGGCATGTGT+TGG	+	chr7_2:59996361-59996380	None:intergenic	30.0%
TGTGCAACTATATACATATC+AGG	-	chr7_2:59996379-59996398	Msa1066050:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	59994180	59998392	59994180	ID=Msa1066050;Name=Msa1066050
chr7_2	mRNA	59994180	59998392	59994180	ID=Msa1066050-mRNA-1;Parent=Msa1066050;Name=Msa1066050-mRNA-1;_AED=0.11;_eAED=0.11;_QI=265\|1\|1\|1\|1\|1\|13\|474\|471
chr7_2	exon	59994180	59994715	59994180	ID=Msa1066050-mRNA-1:exon:11669;Parent=Msa1066050-mRNA-1
chr7_2	exon	59994795	59994897	59994795	ID=Msa1066050-mRNA-1:exon:11668;Parent=Msa1066050-mRNA-1
chr7_2	exon	59995019	59995102	59995019	ID=Msa1066050-mRNA-1:exon:11667;Parent=Msa1066050-mRNA-1
chr7_2	exon	59995195	59995290	59995195	ID=Msa1066050-mRNA-1:exon:11666;Parent=Msa1066050-mRNA-1
chr7_2	exon	59995394	59995444	59995394	ID=Msa1066050-mRNA-1:exon:11665;Parent=Msa1066050-mRNA-1
chr7_2	exon	59995626	59995766	59995626	ID=Msa1066050-mRNA-1:exon:11664;Parent=Msa1066050-mRNA-1
chr7_2	exon	59995852	59995908	59995852	ID=Msa1066050-mRNA-1:exon:11663;Parent=Msa1066050-mRNA-1
chr7_2	exon	59996015	59996089	59996015	ID=Msa1066050-mRNA-1:exon:11662;Parent=Msa1066050-mRNA-1
chr7_2	exon	59996172	59996444	59996172	ID=Msa1066050-mRNA-1:exon:11661;Parent=Msa1066050-mRNA-1
chr7_2	exon	59996570	59996629	59996570	ID=Msa1066050-mRNA-1:exon:11660;Parent=Msa1066050-mRNA-1
chr7_2	exon	59996855	59996947	59996855	ID=Msa1066050-mRNA-1:exon:11659;Parent=Msa1066050-mRNA-1
chr7_2	exon	59997038	59997295	59997038	ID=Msa1066050-mRNA-1:exon:11658;Parent=Msa1066050-mRNA-1
chr7_2	exon	59998065	59998392	59998065	ID=Msa1066050-mRNA-1:exon:11657;Parent=Msa1066050-mRNA-1
chr7_2	five_prime_UTR	59998128	59998392	59998128	ID=Msa1066050-mRNA-1:five_prime_utr;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59998065	59998127	59998065	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59997038	59997295	59997038	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59996855	59996947	59996855	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59996570	59996629	59996570	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59996172	59996444	59996172	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59996015	59996089	59996015	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59995852	59995908	59995852	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59995626	59995766	59995626	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59995394	59995444	59995394	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59995195	59995290	59995195	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59995019	59995102	59995019	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59994795	59994897	59994795	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	CDS	59994654	59994715	59994654	ID=Msa1066050-mRNA-1:cds;Parent=Msa1066050-mRNA-1
chr7_2	three_prime_UTR	59994180	59994653	59994180	ID=Msa1066050-mRNA-1:three_prime_utr;Parent=Msa1066050-mRNA-1

Gene Sequence

>Msa1066050

ATGAATATGATGAGAAGGTTGAAAAGCATTGCTTCTGGTAGAACCTCTGTTTCATCAGATCCTGGAGGGGATTCTACAACAAAAAGAGCCAAGTTTGATTTAGAAACTGAAGGAAAGGAAAATGAAGAAACAAACCGTGTTGAGAGGGGTGGAAATAATCAGGATAAGTATGTAGATGCATCACAAGAAGAGACATCTAGTTCTTGTAATTCTAATGTTTCTGCGGTGGCTAAAACCGAGAAGTCAGGTTTTGATCAGCTTCCAAAAGAATTACACGAAATGAAAATTAAAGATGATAAAAGCAAAAACAACAATGAAAAGGATATGGAGGCAACTGTTGTGAACGGAAATGGAACGGAAACTGGTCAAGTAATTACAACTTCTATTGGTGGTCGAGATGGACAACCAAAGAGGATAATCTCATACATGGCAGAACGTGTCGTTGGTACCGGTTCTTTTGGCGTTGTCTATCAGGCTAAGTGCGTTGAAACTGGCGAATCTGTTGCAATAAAGAAAGTCTTACAAGACAAGAGATATAAAAACAGGGAACTCCAGGTTATGCGTATGCTTGAACATACTAATGTTCTTAAACTAAAGCACTGTTTCTACTCGACGGCTGAAAAAGATGAGGTGTACCTTAACTTAGTTTTGGAGTTTGTACCTGAAACTGTCTACAGAGTTTCAAAGCACTATGTCAGGATGCACCAACACATGCCTATAATTTATGTGCAACTATATACATATCAGATATGCCGCGGTTTAAATTATTTGCATCATGTGGTTGGAGTCTGTCACCGTGACATAAAACCTCAGAATCTATTGGTTAATCCCGTGTGTCATCAGTTAAAGATATGTGATTTTGGTAGTGCAAAAATGTTGTTGCCCGGTGAACCCAACATATCGTACATATGTTCGCGGTATTATAGAGCTCCTGAACTTATATTTGGTGCAACTGAATACACAACTGCTATTGATGTTTGGTCTGCTGGCTGTGTTTTGGCTGAGCTTCTTCTAGGACAGCCGTTGTTTCCCGGAGAGAGTGGGGTTGATCAGTTGGTAGAGATCATTAAGATCTTGGGAACACCTACCAGAGAAGAAATAAAGTGCATGAATCCAAATTACAGTGAATTTAAGTTTCCACAGATTAAAGCTCACCCATGGCACAAGATCTTTCACAAGAGCATGCCTTCTGAAGCAGTGGATCTTGTGTCGAGGATGCTTCAATATTCTCCAAATCTACGCTGCACTGCTTTGGATGCATGTGCACATTCCTTCTTTGATGACCTTCGGGACCCAAACGCCCGGTTACCGAATGGACAAGAACTTCCCCCTTTGTTTGATTTCACAGCCCAAGAGTTAGCAGGCGCACCTGATGAGTTGCGTCGACGTCTAATTCCTGAGCATGCAAGGAGTTGA

Protein sequence

>Msa1066050

MNMMRRLKSIASGRTSVSSDPGGDSTTKRAKFDLETEGKENEETNRVERGGNNQDKYVDASQEETSSSCNSNVSAVAKTEKSGFDQLPKELHEMKIKDDKSKNNNEKDMEATVVNGNGTETGQVITTSIGGRDGQPKRIISYMAERVVGTGSFGVVYQAKCVETGESVAIKKVLQDKRYKNRELQVMRMLEHTNVLKLKHCFYSTAEKDEVYLNLVLEFVPETVYRVSKHYVRMHQHMPIIYVQLYTYQICRGLNYLHHVVGVCHRDIKPQNLLVNPVCHQLKICDFGSAKMLLPGEPNISYICSRYYRAPELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYSEFKFPQIKAHPWHKIFHKSMPSEAVDLVSRMLQYSPNLRCTALDACAHSFFDDLRDPNARLPNGQELPPLFDFTAQELAGAPDELRRRLIPEHARS*