AlfalfaGEDB | Msa1069080

M. sativa cultivar zhongmu-4 / Msa1069080

KEGG: K04733
GO: -
PFAM: PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069080	XP_003624353.1	88.283	862	67	2	1	828	1	862	0.0	1484

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069080	sp\|P43298\|TMK1_ARATH	34.931	793	427	25	27	754	26	794	5.13e-121	391

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069080	G7KUC1	88.283	862	67	2	1	828	1	862	0.0	1484

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1030140	Msa1069080	0.805440	1.333412e-49	-8.615850e-47
Msa1069080	Msa1156950	0.916634	1.406659e-85	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069080	MtrunA17_Chr7g0250931	88.283	862	67	2	1	828	1	862	0.0	1484
Msa1069080	MtrunA17_Chr7g0250741	56.214	861	313	14	7	826	9	846	0.0	881
Msa1069080	MtrunA17_Chr4g0022331	56.014	848	314	13	1	811	4	829	0.0	879
Msa1069080	MtrunA17_Chr7g0238401	54.030	670	229	14	183	808	1	635	0.0	624
Msa1069080	MtrunA17_Chr7g0251071	67.237	409	92	7	459	826	259	666	0.0	538
Msa1069080	MtrunA17_Chr1g0212821	66.667	381	91	4	481	826	15	394	7.01e-178	517
Msa1069080	MtrunA17_Chr7g0251051	68.490	384	83	4	480	826	243	625	3.77e-176	522
Msa1069080	MtrunA17_Chr7g0251001	68.817	372	77	4	493	826	2	372	8.93e-176	511
Msa1069080	MtrunA17_Chr7g0251101	65.327	398	86	5	480	826	275	671	1.62e-173	516
Msa1069080	MtrunA17_Chr7g0251061	66.842	380	90	4	480	824	280	658	2.05e-171	510

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1069080	AT1G66150.1	34.931	793	427	25	27	754	26	794	5.22e-122	391
Msa1069080	AT2G01820.1	33.375	806	456	21	8	754	8	791	2.14e-118	381
Msa1069080	AT4G18250.1	47.739	398	142	10	480	828	300	680	9.77e-114	362
Msa1069080	AT1G66920.2	49.740	384	135	7	478	820	245	611	2.92e-111	353
Msa1069080	AT1G66920.1	49.738	382	134	7	480	820	239	603	5.26e-111	352
Msa1069080	AT1G66910.2	48.259	402	154	9	460	821	264	651	6.08e-110	351
Msa1069080	AT1G70250.1	53.453	333	109	6	533	826	444	769	1.22e-109	354
Msa1069080	AT5G39020.1	50.000	376	136	6	488	828	449	807	2.27e-109	354
Msa1069080	AT1G66980.4	51.988	327	115	4	533	821	773	1095	5.17e-109	360
Msa1069080	AT1G66980.1	51.988	327	115	4	533	821	790	1112	8.34e-109	359

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Find 194 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTATTGACAACAATG+TGG	0.676647	7_2:-64603442	Msa1069080:CDS
AAAATTATAAATGAGTCAAA+GGG	0.524654	7_2:-64603261	Msa1069080:CDS
AAAGTATTAGAAATGCTACA+AGG	0.661359	7_2:-64602545	Msa1069080:CDS
AAATATGAAGTTTGCAATGA+TGG	0.410678	7_2:+64602467	None:intergenic
AAATTAGGTCAAGGAGGATA+TGG	0.363645	7_2:-64603330	Msa1069080:CDS
AACACAACTGCTTCTCGTGA+TGG	0.532304	7_2:-64604551	Msa1069080:CDS
AACCATGTGAATACTTCTGA+TGG	0.560353	7_2:+64605394	None:intergenic
AACCGTGACAATGATGATGA+TGG	0.575761	7_2:+64605891	None:intergenic
AACTCGTTCCGAGATAAATT+AGG	0.446841	7_2:-64603345	Msa1069080:CDS
AACTTATCCAATGATCCTTT+GGG	0.400145	7_2:+64603120	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTGAAGTTATTGTAACCA+AGG	+	chr7_2:64602992-64603011	None:intergenic	30.0%
TGATATAACATCAATTGCAC+CGG	+	chr7_2:64603318-64603337	None:intergenic	30.0%
CTTCAATCAAATTCCTTAAC+TGG	-	chr7_2:64603434-64603453	Msa1069080:CDS	30.0%
ATCAAATTCCTTAACTGGTT+TGG	-	chr7_2:64603439-64603458	Msa1069080:CDS	30.0%
ACATTAAGAAGTTGAAGGTT+TGG	+	chr7_2:64603878-64603897	None:intergenic	30.0%
CAGAAACATTAAGAAGTTGA+AGG	+	chr7_2:64603883-64603902	None:intergenic	30.0%
GTTTCTGATAATAATCTGTC+TGG	-	chr7_2:64603896-64603915	Msa1069080:intron	30.0%
GAAATATTTAACCCAAGCAA+AGG	+	chr7_2:64603971-64603990	None:intergenic	30.0%
AATATTTCCCAACAATTAGG+TGG	-	chr7_2:64603983-64604002	Msa1069080:intron	30.0%
GTGGCAAATATGTATATACT+TGG	-	chr7_2:64604136-64604155	Msa1069080:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	64602427	64606159	64602427	ID=Msa1069080;Name=Msa1069080
chr7_2	mRNA	64602427	64606159	64602427	ID=Msa1069080-mRNA-1;Parent=Msa1069080;Name=Msa1069080-mRNA-1;_AED=0.06;_eAED=0.06;_QI=206\|0.5\|0.33\|1\|1\|1\|3\|0\|828
chr7_2	exon	64602427	64602587	64602427	ID=Msa1069080-mRNA-1:exon:13493;Parent=Msa1069080-mRNA-1
chr7_2	exon	64602683	64603564	64602683	ID=Msa1069080-mRNA-1:exon:13492;Parent=Msa1069080-mRNA-1
chr7_2	exon	64604510	64606159	64604510	ID=Msa1069080-mRNA-1:exon:13491;Parent=Msa1069080-mRNA-1
chr7_2	five_prime_UTR	64605954	64606159	64605954	ID=Msa1069080-mRNA-1:five_prime_utr;Parent=Msa1069080-mRNA-1
chr7_2	CDS	64604510	64605953	64604510	ID=Msa1069080-mRNA-1:cds;Parent=Msa1069080-mRNA-1
chr7_2	CDS	64602683	64603564	64602683	ID=Msa1069080-mRNA-1:cds;Parent=Msa1069080-mRNA-1
chr7_2	CDS	64602427	64602587	64602427	ID=Msa1069080-mRNA-1:cds;Parent=Msa1069080-mRNA-1

Gene Sequence

>Msa1069080

ATGAAAACATTGTCCCAACAATCAATTTTGTACTATTTTTCCATCATCATCATTGTCACGGTTACTATAACTTATGGAGAGAAAGATGCAGATTACATGTCCCAACTTATGAAAGCACTGACCCCAACTCCCAAAGGCTGGTCTAGCAACATTCACTACTGCAAATGGAACGGCATCATGTGCGACCAATCTAATCAAGTTGTCACCGCCATCAAGCTTCCATCAAGTTCACTCACTGGAACAATCCCTCAGAATTTCAACTCTCTCAATAATCTCACCGACATCGATCTTCATAACAACTCACTCAACGGTCCTTTGCCTGATTTGTCTTTCCTAAGCGCGCTCCAAACCGTGAACCTTGGTTACAATAACTTCACTTCAATCCCAGACTTTTGCTTTGGACCCCTACCAAATCTAACAACCCTCAACCTTAGCAATAACTTGAATCTCATGCCATGGTTATTTCATGATGAATTGTCCGCTTCCTCGTTGATACATACCCTCGATCTCGAAGCAACAAATCTCATAGGTCCATTACCATCAGAAGTATTCACATGGTTTCCGCGTTTGCATACTGTTTTTCTTTCTCACAACAACTTAAGTGGAACTCTTCCCCCTTCTCTTGGGGAATCTTCAATAAGGTATTTGCGACTCAATGACCAAGGGCCGTTTAGTGGGTTTACCGGTGCAATTGATGTTATATCATCTATGAGGTTCTTGTCTCAGGCGTGGCTTAACAACAACAACTTCGCAGGTCCAATTCCAAACATGTCTAGTTCCACTAATCTGTTTGATCTTCAGCTTCAATCAAATTCCTTAACTGGTTTGGTTCCACATTCGCTATGCACTCTCTCTAGCTTGAAGAACATCTCTTTGGACAGCAATTATCTTCAAGGACCGATACCTGTGTTTCACGAGGGTGTTAATGCAAGTTGGAAAGGAAATTACTTTTGTCGAAATGATGTTGGACCTTGTGATCCCCAAGTTACAATTTTGCTGGAGATTCTTGGAGCTGTTGGGTATCCTTCTTTTATGTCAAATTATGAAGGGAACAAAAACGATGCTTGTTCAGGAGACGAATATTTAAAGTGTTCAAAAGGGAAGATTGTGGTCTTTCATTTGGACGGTCTGCATCTAGGCGGAACAATCTCTCCAGCGTTTTCTGGTTTATCAAGTTTAGTGAACTTGACTCTTGGTAGCAATAATTTAACGGGTTCAATACCACATAGTCTTACAACTTTGCCAAACCTTCAACTTCTTAATGTTTCTGATAATAATCTGTCTGGACAAGTTCCCAAATTTTCTTCAAATGTTAAGTTAATTATAAGTGGCAATCCTTTGCTTGGGTTAAATATTTCCCAACAATTAGGTGGAGGAGAAAACACAACTGCTTCTCGTGATGGAGGCTTATCTAAGACAACAATTGAACCTGCTTGGATTGTTGGGGCGTCTTTGCTTAGTGTTGGGTTTGTAACACTAATTGTGATTCTTTGTCGTAAGAGAGATCTTATTTTAGTACAAAGGTGGATCTTGAGGAAAACAACAAAATCTATTGACAACAATGTGGAGGATTTTATACAGACTTATAATTTGTCTGTGCCAATAAAGCAATATAGATATGCAGAAGTGAAAAGAATGACAAACTCGTTCCGAGATAAATTAGGTCAAGGAGGATATGGTGTTGTTTACAAAGCAAACTTACCTGATGGTCGTCAAGTAGCAGTGAAAATTATAAATGAGTCAAAGGGAAATGGAGAAGATTTCATAAATGAAGTTGCTAGCATTAGTAGAACGTCACATGTGAACATCGTCTCGCTTTTGGGATTTTGTTATGAGAACAAAAGAGCACTAATATATGAGTTCTTGCCCAAAGGATCATTGGATAAGTTCATTCTTAAAAGTGGATTTCATGATGCTATTTGTAGTTTGGATTGGAACACATTGTACCAAATTGCAATCGGAATTGCTCGAGGACTAGAATACTTGCATCAAGGATGTACTTCAAGAATTTTGCATCTTGATATCAAACCACAAAACATTCTTTTGGATGAAAATTTTTGCCCCAAAATCTCTGATTTTGGACTAGCTAAAGTATGCCAAAGGAATGATAGTATTGTGTCTTTACTTGGCACAAGAGGAACTATAGGATATATAGCACCAGAAGTATTCAGCCGAACATATGGTGGAGTTTCTCACAAGTCTGATGTATATAGTTATGGTATGTTAATTCTAGAAATGGTTGGGGGAAGAAAGAATTATGACACTGGAGGGTCATGTACTTCTGAAATGTGTTTTCCAGATTGGATTTATAAGGATCTTGAGCAAGCTGATAGACCATCGATGAGTAAAGTATTAGAAATGCTACAAGGACCACTTCAGTCAGTGCCATATCCTCCAAAGCCTTTCTTATATTCTTCTGAAATGCCATCATTGCAAACTTCATATTTGTCTTCCAGCAATTTGTTAGAGACAAACTCTTCATCCTAA

Protein sequence

>Msa1069080

MKTLSQQSILYYFSIIIIVTVTITYGEKDADYMSQLMKALTPTPKGWSSNIHYCKWNGIMCDQSNQVVTAIKLPSSSLTGTIPQNFNSLNNLTDIDLHNNSLNGPLPDLSFLSALQTVNLGYNNFTSIPDFCFGPLPNLTTLNLSNNLNLMPWLFHDELSASSLIHTLDLEATNLIGPLPSEVFTWFPRLHTVFLSHNNLSGTLPPSLGESSIRYLRLNDQGPFSGFTGAIDVISSMRFLSQAWLNNNNFAGPIPNMSSSTNLFDLQLQSNSLTGLVPHSLCTLSSLKNISLDSNYLQGPIPVFHEGVNASWKGNYFCRNDVGPCDPQVTILLEILGAVGYPSFMSNYEGNKNDACSGDEYLKCSKGKIVVFHLDGLHLGGTISPAFSGLSSLVNLTLGSNNLTGSIPHSLTTLPNLQLLNVSDNNLSGQVPKFSSNVKLIISGNPLLGLNISQQLGGGENTTASRDGGLSKTTIEPAWIVGASLLSVGFVTLIVILCRKRDLILVQRWILRKTTKSIDNNVEDFIQTYNLSVPIKQYRYAEVKRMTNSFRDKLGQGGYGVVYKANLPDGRQVAVKIINESKGNGEDFINEVASISRTSHVNIVSLLGFCYENKRALIYEFLPKGSLDKFILKSGFHDAICSLDWNTLYQIAIGIARGLEYLHQGCTSRILHLDIKPQNILLDENFCPKISDFGLAKVCQRNDSIVSLLGTRGTIGYIAPEVFSRTYGGVSHKSDVYSYGMLILEMVGGRKNYDTGGSCTSEMCFPDWIYKDLEQADRPSMSKVLEMLQGPLQSVPYPPKPFLYSSEMPSLQTSYLSSSNLLETNSSS*