Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1069200 | AES80585.2 | 95.251 | 358 | 17 | 0 | 1 | 358 | 293 | 650 | 0.0 | 714 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1069200 | sp|Q9FF29|PR5K_ARATH | 58.104 | 327 | 124 | 4 | 12 | 335 | 309 | 625 | 6.89e-134 | 399 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1069200 | Msa1157080 | 0.864146 | 1.499904e-64 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1069200 | MtrunA17_Chr7g0251071 | 95.251 | 358 | 17 | 0 | 1 | 358 | 314 | 671 | 0.0 | 714 |
| Msa1069200 | MtrunA17_Chr7g0251051 | 91.808 | 354 | 28 | 1 | 1 | 354 | 274 | 626 | 0.0 | 669 |
| Msa1069200 | MtrunA17_Chr7g0251001 | 87.606 | 355 | 44 | 0 | 3 | 357 | 22 | 376 | 0.0 | 656 |
| Msa1069200 | MtrunA17_Chr7g0251101 | 85.994 | 357 | 49 | 1 | 2 | 358 | 321 | 676 | 0.0 | 642 |
| Msa1069200 | MtrunA17_Chr7g0251061 | 86.592 | 358 | 47 | 1 | 1 | 358 | 309 | 665 | 0.0 | 642 |
| Msa1069200 | MtrunA17_Chr1g0212821 | 85.154 | 357 | 52 | 1 | 2 | 358 | 44 | 399 | 0.0 | 632 |
| Msa1069200 | MtrunA17_Chr7g0250931 | 78.693 | 352 | 72 | 3 | 3 | 353 | 511 | 860 | 0.0 | 580 |
| Msa1069200 | MtrunA17_Chr4g0022331 | 79.217 | 332 | 68 | 1 | 3 | 334 | 495 | 825 | 0.0 | 569 |
| Msa1069200 | MtrunA17_Chr7g0250741 | 75.630 | 357 | 85 | 2 | 3 | 358 | 496 | 851 | 0.0 | 563 |
| Msa1069200 | MtrunA17_Chr7g0251081 | 81.081 | 333 | 46 | 2 | 1 | 333 | 86 | 401 | 0.0 | 553 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1069200 | AT5G38280.1 | 58.104 | 327 | 124 | 4 | 12 | 335 | 309 | 625 | 7.01e-135 | 399 |
| Msa1069200 | AT5G38280.2 | 58.104 | 327 | 124 | 4 | 12 | 335 | 309 | 625 | 1.28e-134 | 399 |
| Msa1069200 | AT1G70250.1 | 59.032 | 310 | 119 | 4 | 28 | 333 | 447 | 752 | 7.06e-128 | 385 |
| Msa1069200 | AT5G38260.1 | 52.089 | 359 | 150 | 6 | 3 | 354 | 285 | 628 | 2.30e-127 | 379 |
| Msa1069200 | AT4G18250.1 | 52.222 | 360 | 147 | 8 | 6 | 350 | 326 | 675 | 1.54e-126 | 379 |
| Msa1069200 | AT5G39020.1 | 55.385 | 325 | 133 | 3 | 6 | 328 | 466 | 780 | 2.50e-126 | 382 |
| Msa1069200 | AT1G66980.4 | 56.634 | 309 | 121 | 4 | 28 | 330 | 776 | 1077 | 4.98e-125 | 385 |
| Msa1069200 | AT1G66980.1 | 56.634 | 309 | 121 | 4 | 28 | 330 | 793 | 1094 | 9.54e-125 | 385 |
| Msa1069200 | AT5G38240.3 | 52.836 | 335 | 144 | 3 | 1 | 333 | 234 | 556 | 1.56e-124 | 370 |
| Msa1069200 | AT1G66980.2 | 56.634 | 309 | 121 | 4 | 28 | 330 | 822 | 1123 | 2.06e-124 | 385 |
Find 65 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACACGTTGACAACAATG+TGG | 0.720631 | 7_2:-64715524 | Msa1069200:CDS |
| AAACGAGTCAAAAGGAAACG+GGG | 0.713273 | 7_2:-64715338 | Msa1069200:CDS |
| AAAGTAATAAACGAGTCAAA+AGG | 0.516105 | 7_2:-64715346 | Msa1069200:CDS |
| AAAGTAATAGAAATGCTGCA+AGG | 0.659872 | 7_2:-64714620 | Msa1069200:CDS |
| AAATCTGATGTATATAGTTA+TGG | 0.448856 | 7_2:-64714872 | Msa1069200:CDS |
| AAATTAGGTCACGGAGGATA+TGG | 0.347673 | 7_2:-64715415 | Msa1069200:CDS |
| AAATTGTTTGACAATCTCTG+AGG | 0.700315 | 7_2:-64714726 | Msa1069200:CDS |
| AACTTATCCAGGGATCCTTT+GGG | 0.441285 | 7_2:+64715202 | None:intergenic |
| AATGCTCTACTAAATACTTC+TGG | 0.418272 | 7_2:+64714911 | None:intergenic |
| AATTCATTTCGAGATAAATT+AGG | 0.325082 | 7_2:-64715430 | Msa1069200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTCGAGATAAATTAGGTCA+CGG | - | chr7_2:64714616-64714635 | Msa1069200:CDS | 30.0% | |
| TGTGTACAAAGCATGTTTAA+CGG | - | chr7_2:64714651-64714670 | Msa1069200:CDS | 30.0% | |
| ATAAACGAGTCAAAAGGAAA+CGG | - | chr7_2:64714700-64714719 | Msa1069200:CDS | 30.0% | |
| ATATATGAATACATGCCCAA+AGG | - | chr7_2:64714823-64714842 | Msa1069200:CDS | 30.0% | |
| CTTATAGATGAACTTATCCA+GGG | + | chr7_2:64714851-64714870 | None:intergenic | 30.0% | |
| TCTTATAGATGAACTTATCC+AGG | + | chr7_2:64714852-64714871 | None:intergenic | 30.0% | |
| GATAAGTTCATCTATAAGAG+TGG | - | chr7_2:64714853-64714872 | Msa1069200:CDS | 30.0% | |
| ATTTCAATTGGAATTGCTAG+AGG | - | chr7_2:64714922-64714941 | Msa1069200:CDS | 30.0% | |
| TGCTAAAATATGTCAAAGGA+AGG | - | chr7_2:64715056-64715075 | Msa1069200:CDS | 30.0% | |
| AATGCTCTACTAAATACTTC+TGG | + | chr7_2:64715132-64715151 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 64714493 | 64715569 | 64714493 | ID=Msa1069200;Name=Msa1069200 |
| chr7_2 | mRNA | 64714493 | 64715569 | 64714493 | ID=Msa1069200-mRNA-1;Parent=Msa1069200;Name=Msa1069200-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|358 |
| chr7_2 | exon | 64714493 | 64715569 | 64714493 | ID=Msa1069200-mRNA-1:exon:13521;Parent=Msa1069200-mRNA-1 |
| chr7_2 | CDS | 64714493 | 64715569 | 64714493 | ID=Msa1069200-mRNA-1:cds;Parent=Msa1069200-mRNA-1 |
| Gene Sequence |
>Msa1069200 ATGGCAATTTTCAGGAAAAGAAGAAAACACGTTGACAACAATGTGGAGGTTTTCATGCAAAGTTACAATTTATCGATTGCGAGAAGATACAATTATACAGAAGTAAAAAGGATCACAAATTCATTTCGAGATAAATTAGGTCACGGAGGATATGGTGTTGTGTACAAAGCATGTTTAACGGATGGTCGTCAAGTGGCAGTGAAAGTAATAAACGAGTCAAAAGGAAACGGGGAAGAGTTCATAAATGAAGTTGCTAGCATTAGTAGAACATCACACATGAACATTGTGTCACTTTTAGGTTATTGTTATGAGGTGAATAAAAGAGCGCTAATATATGAATACATGCCCAAAGGATCCCTGGATAAGTTCATCTATAAGAGTGGATTTCCTGACGCTGTTTGTGATTTTGATTGGAACACTTTGTTCCAAATTTCAATTGGAATTGCTAGAGGACTAGAGTACTTGCATCAAGGATGTAGTTCAAGGATTTTGCATCTTGATATCAAACCTCAAAACATTCTTTTGGATGAAGACTTTTGTCCAAAAATATCTGATTTTGGACTTGCTAAAATATGTCAAAGGAAGGACAGTACTGTGTCAATATTGGGGACACGAGGAACTATAGGATATATGGCGCCAGAAGTATTTAGTAGAGCATTTGGTGCAGTTTCTTACAAATCTGATGTATATAGTTATGGCATGTTGATTCTTGAAATGATCGGAGGAAGGAAGAACTATGACACTGGAGGGTCATGTACTTCTGAAATGTACTTTCCAGATTGGATTTATAAGGATCTCGAACAAGGTAGCCATACTGTTTTAAATTGTTTGACAATCTCTGAGGAAGAAAATGATATGGTTAGAAAGATTACTATGGTGAGTCTATGGTGCATTCAAACGAATCCATCAGATAGACCTCCAATGAATAAAGTAATAGAAATGCTGCAAGGACCACTCTCATCTGTGTCATATCCTCCAAAGCCTGTCTTATTTTCTCCAGAAAGGCCTCCACAACAATTGTCATATATGTCTTCATCTGATTTTTATGAGACAAATTCAATTACACTGTCAAAATAA |
| Protein sequence |
>Msa1069200 MAIFRKRRKHVDNNVEVFMQSYNLSIARRYNYTEVKRITNSFRDKLGHGGYGVVYKACLTDGRQVAVKVINESKGNGEEFINEVASISRTSHMNIVSLLGYCYEVNKRALIYEYMPKGSLDKFIYKSGFPDAVCDFDWNTLFQISIGIARGLEYLHQGCSSRILHLDIKPQNILLDEDFCPKISDFGLAKICQRKDSTVSILGTRGTIGYMAPEVFSRAFGAVSYKSDVYSYGMLILEMIGGRKNYDTGGSCTSEMYFPDWIYKDLEQGSHTVLNCLTISEEENDMVRKITMVSLWCIQTNPSDRPPMNKVIEMLQGPLSSVSYPPKPVLFSPERPPQQLSYMSSSDFYETNSITLSK* |
