Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1073690 | ACJ84052.1 | 96.296 | 108 | 4 | 0 | 1 | 108 | 1 | 108 | 1.48e-70 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1073690 | sp|O48917|SQD1_ARATH | 50.000 | 114 | 49 | 4 | 1 | 110 | 1 | 110 | 4.35e-21 | 90.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1073690 | B7FH23 | 96.296 | 108 | 4 | 0 | 1 | 108 | 1 | 108 | 7.05e-71 | 216 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0980440 | Msa1073690 | 0.802849 | 4.614886e-49 | -8.615850e-47 |
| Msa0589150 | Msa1073690 | 0.822259 | 2.607309e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1073690 | MtrunA17_Chr7g0256731 | 96.364 | 110 | 4 | 0 | 1 | 110 | 1 | 110 | 1.45e-72 | 223 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1073690 | AT4G33030.1 | 50.000 | 114 | 49 | 4 | 1 | 110 | 1 | 110 | 4.43e-22 | 90.5 |
Find 31 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACTCGCTTCGGTTTAAA+AGG | 0.187741 | 7_2:-71320629 | None:intergenic |
| CGCAAGAAAAGCCGTCTAAA+TGG | 0.294684 | 7_2:-71320515 | None:intergenic |
| TAGTTTGAACCGGGGCTTCC+TGG | 0.305214 | 7_2:-71320595 | None:intergenic |
| GCCCCGGTTCAAACTAGTTC+TGG | 0.371819 | 7_2:+71320602 | Msa1073690:CDS |
| ACAGAAACCAAGGAAAAGTT+TGG | 0.427205 | 7_2:+71320541 | Msa1073690:CDS |
| AAATTCAAGTCTCAAAATCT+TGG | 0.433501 | 7_2:-71320868 | None:intergenic |
| CCTCGTAACCTTTGTTAGAA+AGG | 0.451936 | 7_2:-71320697 | None:intergenic |
| CCAATGACCATTACTCGCTT+CGG | 0.452722 | 7_2:-71320638 | None:intergenic |
| GATGTTGAGCATTGGTGAAA+AGG | 0.453224 | 7_2:-71320455 | None:intergenic |
| TCGCCAGAACTAGTTTGAAC+CGG | 0.465526 | 7_2:-71320605 | None:intergenic |
| TTCTACAGTTTCAACTGGCC+AGG | 0.466150 | 7_2:+71320577 | Msa1073690:CDS |
| AATGCTTCTACAGTTTCAAC+TGG | 0.486284 | 7_2:+71320572 | Msa1073690:CDS |
| CCGAAGCGAGTAATGGTCAT+TGG | 0.499010 | 7_2:+71320638 | Msa1073690:CDS |
| CGCCAGAACTAGTTTGAACC+GGG | 0.501193 | 7_2:-71320604 | None:intergenic |
| AACCTTTGTTAGAAAGGTGG+AGG | 0.503426 | 7_2:-71320691 | None:intergenic |
| GTAATGGTCATTGGTGGAGA+TGG | 0.511804 | 7_2:+71320647 | Msa1073690:CDS |
| TTCAACTGGCCAGGAAGCCC+CGG | 0.518120 | 7_2:+71320586 | Msa1073690:CDS |
| TTACTCGCTTCGGTTTAAAA+GGG | 0.531134 | 7_2:-71320628 | None:intergenic |
| TGGAGATGGATACTGTGGTT+GGG | 0.542951 | 7_2:+71320661 | Msa1073690:CDS |
| TAAACAAGGTTTGTTTGAAG+CGG | 0.550343 | 7_2:-71320429 | None:intergenic |
| GTGGAGATGGATACTGTGGT+TGG | 0.563462 | 7_2:+71320660 | Msa1073690:CDS |
| CGTAACCTTTGTTAGAAAGG+TGG | 0.578750 | 7_2:-71320694 | None:intergenic |
| TAGAAAATGATGTTGAGCAT+TGG | 0.583068 | 7_2:-71320463 | None:intergenic |
| AAGCGAGTAATGGTCATTGG+TGG | 0.585308 | 7_2:+71320641 | Msa1073690:CDS |
| CCTTTCTAACAAAGGTTACG+AGG | 0.587127 | 7_2:+71320697 | Msa1073690:CDS |
| GTTTGAAGCGGAGAAAGTCA+AGG | 0.597796 | 7_2:-71320417 | None:intergenic |
| GCCCTCCACCTTTCTAACAA+AGG | 0.602875 | 7_2:+71320689 | Msa1073690:CDS |
| ACCTTTGTTAGAAAGGTGGA+GGG | 0.612052 | 7_2:-71320690 | None:intergenic |
| TCTTGCGAGAACAGAAACCA+AGG | 0.641034 | 7_2:+71320531 | Msa1073690:CDS |
| ATTGGTGGAGATGGATACTG+TGG | 0.674313 | 7_2:+71320656 | Msa1073690:CDS |
| GCCAGAACTAGTTTGAACCG+GGG | 0.700751 | 7_2:-71320603 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAACAAGGTTTGTTTGAAG+CGG | - | chr7_2:71320432-71320451 | None:intergenic | 30.0% |
| !!! | TGGTGAAAAGGTTTTAAACA+AGG | - | chr7_2:71320446-71320465 | None:intergenic | 30.0% |
| TAGAAAATGATGTTGAGCAT+TGG | - | chr7_2:71320466-71320485 | None:intergenic | 30.0% | |
| ! | CTCAACATCATTTTCTAACA+CGG | + | chr7_2:71320469-71320488 | Msa1073690:CDS | 30.0% |
| ACTCCAAAAATCCATTTAGA+CGG | + | chr7_2:71320504-71320523 | Msa1073690:CDS | 30.0% | |
| !! | AAGCCGTCTAAATGGATTTT+TGG | - | chr7_2:71320510-71320529 | None:intergenic | 35.0% |
| ACAGAAACCAAGGAAAAGTT+TGG | + | chr7_2:71320541-71320560 | Msa1073690:CDS | 35.0% | |
| AATGCTTCTACAGTTTCAAC+TGG | + | chr7_2:71320572-71320591 | Msa1073690:CDS | 35.0% | |
| TTACTCGCTTCGGTTTAAAA+GGG | - | chr7_2:71320631-71320650 | None:intergenic | 35.0% | |
| ATTACTCGCTTCGGTTTAAA+AGG | - | chr7_2:71320632-71320651 | None:intergenic | 35.0% | |
| ! | TTTTAAACCGAAGCGAGTAA+TGG | + | chr7_2:71320631-71320650 | Msa1073690:CDS | 35.0% |
| !! | GATGTTGAGCATTGGTGAAA+AGG | - | chr7_2:71320458-71320477 | None:intergenic | 40.0% |
| ! | ACAACTGCCAAACTTTTCCT+TGG | - | chr7_2:71320551-71320570 | None:intergenic | 40.0% |
| ACCTTTGTTAGAAAGGTGGA+GGG | - | chr7_2:71320693-71320712 | None:intergenic | 40.0% | |
| AACCTTTGTTAGAAAGGTGG+AGG | - | chr7_2:71320694-71320713 | None:intergenic | 40.0% | |
| CGTAACCTTTGTTAGAAAGG+TGG | - | chr7_2:71320697-71320716 | None:intergenic | 40.0% | |
| CCTCGTAACCTTTGTTAGAA+AGG | - | chr7_2:71320700-71320719 | None:intergenic | 40.0% | |
| CCTTTCTAACAAAGGTTACG+AGG | + | chr7_2:71320697-71320716 | Msa1073690:CDS | 40.0% | |
| ! | GTTTGAAGCGGAGAAAGTCA+AGG | - | chr7_2:71320420-71320439 | None:intergenic | 45.0% |
| CGCAAGAAAAGCCGTCTAAA+TGG | - | chr7_2:71320518-71320537 | None:intergenic | 45.0% | |
| TCTTGCGAGAACAGAAACCA+AGG | + | chr7_2:71320531-71320550 | Msa1073690:CDS | 45.0% | |
| TTCTACAGTTTCAACTGGCC+AGG | + | chr7_2:71320577-71320596 | Msa1073690:CDS | 45.0% | |
| ! | TCGCCAGAACTAGTTTGAAC+CGG | - | chr7_2:71320608-71320627 | None:intergenic | 45.0% |
| CCAATGACCATTACTCGCTT+CGG | - | chr7_2:71320641-71320660 | None:intergenic | 45.0% | |
| AAGCGAGTAATGGTCATTGG+TGG | + | chr7_2:71320641-71320660 | Msa1073690:CDS | 45.0% | |
| GTAATGGTCATTGGTGGAGA+TGG | + | chr7_2:71320647-71320666 | Msa1073690:CDS | 45.0% | |
| ATTGGTGGAGATGGATACTG+TGG | + | chr7_2:71320656-71320675 | Msa1073690:CDS | 45.0% | |
| TGGAGATGGATACTGTGGTT+GGG | + | chr7_2:71320661-71320680 | Msa1073690:CDS | 45.0% | |
| ! | GCCAGAACTAGTTTGAACCG+GGG | - | chr7_2:71320606-71320625 | None:intergenic | 50.0% |
| ! | CGCCAGAACTAGTTTGAACC+GGG | - | chr7_2:71320607-71320626 | None:intergenic | 50.0% |
| CCGAAGCGAGTAATGGTCAT+TGG | + | chr7_2:71320638-71320657 | Msa1073690:CDS | 50.0% | |
| GTGGAGATGGATACTGTGGT+TGG | + | chr7_2:71320660-71320679 | Msa1073690:CDS | 50.0% | |
| GCCCTCCACCTTTCTAACAA+AGG | + | chr7_2:71320689-71320708 | Msa1073690:CDS | 50.0% | |
| TAGTTTGAACCGGGGCTTCC+TGG | - | chr7_2:71320598-71320617 | None:intergenic | 55.0% | |
| GCCCCGGTTCAAACTAGTTC+TGG | + | chr7_2:71320602-71320621 | Msa1073690:CDS | 55.0% | |
| CTCGAAGTGATTCTCCCACC+AGG | - | chr7_2:71320834-71320853 | None:intergenic | 55.0% | |
| TTCAACTGGCCAGGAAGCCC+CGG | + | chr7_2:71320586-71320605 | Msa1073690:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 71320389 | 71320886 | 71320389 | ID=Msa1073690;Name=Msa1073690 |
| chr7_2 | mRNA | 71320389 | 71320886 | 71320389 | ID=Msa1073690-mRNA-1;Parent=Msa1073690;Name=Msa1073690-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0|0|0|0.5|1|1|2|0|124 |
| chr7_2 | exon | 71320389 | 71320718 | 71320389 | ID=Msa1073690-mRNA-1:exon:16137;Parent=Msa1073690-mRNA-1 |
| chr7_2 | exon | 71320842 | 71320886 | 71320842 | ID=Msa1073690-mRNA-1:exon:16138;Parent=Msa1073690-mRNA-1 |
| chr7_2 | CDS | 71320389 | 71320718 | 71320389 | ID=Msa1073690-mRNA-1:cds;Parent=Msa1073690-mRNA-1 |
| chr7_2 | CDS | 71320842 | 71320886 | 71320842 | ID=Msa1073690-mRNA-1:cds;Parent=Msa1073690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
