AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1074410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1074410 MtrunA17_Chr7g0257651 97.500 80 2 0 1 80 1 80 5.13e-56 167
Msa1074410 MtrunA17_Chr4g0054381 95.000 80 4 0 1 80 1 80 7.40e-55 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1074410 AT1G01170.1 69.737 76 23 0 4 79 8 83 1.22e-34 113
Msa1074410 AT1G01170.2 69.737 76 23 0 4 79 8 83 1.22e-34 113
Msa1074410 AT4G00860.1 58.667 75 31 0 5 79 6 80 9.97e-30 100

Find 41 sgRNAs with CRISPR-Local

Find 301 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATCGTAGCTTCCTTAAT+TGG 0.167088 7_2:-72231261 Msa1074410:CDS
GTCTTAATTTGGTTGTTTAT+TGG 0.189602 7_2:-72230952 Msa1074410:exon
TTGCAGTTCAATTACTGTTT+GGG 0.235784 7_2:-72230856 Msa1074410:three_prime_UTR
TAAGATTAGCAGAGTTGTTT+AGG 0.262770 7_2:-72230650 Msa1074410:three_prime_UTR
ATGAAATTCTGGTGACTAAT+AGG 0.263292 7_2:+72230993 None:intergenic
CTTGCAGTTCAATTACTGTT+TGG 0.267490 7_2:-72230857 Msa1074410:three_prime_UTR
TCTCGCCCTGAGTCTTAATT+TGG 0.281308 7_2:-72230963 Msa1074410:exon
GCTTCCTTAATTGGATCATT+TGG 0.303375 7_2:-72231252 Msa1074410:CDS
ATAAAATGTTGAAGCAATCT+TGG 0.343315 7_2:-72230800 Msa1074410:three_prime_UTR
AATTCCAAATGATCCAATTA+AGG 0.363857 7_2:+72231248 None:intergenic
GAGACTTCACAATGAAATTC+TGG 0.366406 7_2:+72230982 None:intergenic
AAGATTAGCAGAGTTGTTTA+GGG 0.371807 7_2:-72230649 Msa1074410:three_prime_UTR
CAGTCTCTTCTTGCCATGCC+GGG 0.386027 7_2:+72231069 None:intergenic
TCAGTCTCTTCTTGCCATGC+CGG 0.397382 7_2:+72231068 None:intergenic
ACAAACAGATCTCAAGCTTC+AGG 0.408727 7_2:-72232790 Msa1074410:intron
AAGACAAACATCATTTAGAA+AGG 0.416503 7_2:-72230576 Msa1074410:three_prime_UTR
GCTACGAGCAGCACGAATCT+TGG 0.422689 7_2:-72230741 Msa1074410:three_prime_UTR
AATGCATGGCCTCGCACTGC+TGG 0.442810 7_2:-72231035 Msa1074410:CDS
TAATTGGTTCACCTTGGTCA+TGG 0.497418 7_2:-72230921 Msa1074410:three_prime_UTR
AGACAAACATCATTTAGAAA+GGG 0.501260 7_2:-72230575 Msa1074410:three_prime_UTR
TTGGATCATTTGGAATTGCA+TGG 0.501667 7_2:-72231242 Msa1074410:CDS
TGCAGTTCAATTACTGTTTG+GGG 0.504345 7_2:-72230855 Msa1074410:three_prime_UTR
AAACAACCAAATTAAGACTC+AGG 0.506665 7_2:+72230957 None:intergenic
AGCAAAGAAAATCAATGTAA+AGG 0.515402 7_2:+72230616 None:intergenic
TTGAATTAATTGGTTCACCT+TGG 0.519994 7_2:-72230927 Msa1074410:three_prime_UTR
TTAGAAAGGGCATTCACCAT+GGG 0.521658 7_2:-72230562 Msa1074410:three_prime_UTR
CTAATAGGATTCATGACGAC+TGG 0.528713 7_2:+72231008 None:intergenic
CCATGCCGGGTTTGAAGCTG+TGG 0.542307 7_2:+72231082 None:intergenic
TTTAGAAAGGGCATTCACCA+TGG 0.552149 7_2:-72230563 Msa1074410:three_prime_UTR
AGCTGAAACAATGACTGTAA+AGG 0.553557 7_2:+72230701 None:intergenic
CCACAGCTTCAAACCCGGCA+TGG 0.563894 7_2:-72231082 Msa1074410:CDS
GCAGTTCAATTACTGTTTGG+GGG 0.574197 7_2:-72230854 Msa1074410:three_prime_UTR
AACAACCAAATTAAGACTCA+GGG 0.584833 7_2:+72230958 None:intergenic
TTTCTGCTCCGAGAGAGCCA+GGG 0.606937 7_2:+72232852 None:intergenic
CTGTGCCACAGCTTCAAACC+CGG 0.607354 7_2:-72231087 Msa1074410:CDS
ATAGAACTTCAAAATAACAT+TGG 0.618235 7_2:+72230677 None:intergenic
CTGACAAGAAGTTTAATGCA+TGG 0.646285 7_2:-72231049 Msa1074410:CDS
GTTGACATATTCCATGACCA+AGG 0.646594 7_2:+72230910 None:intergenic
ATAGGATTCATGACGACTGG+AGG 0.649182 7_2:+72231011 None:intergenic
GCAAAGGGAATGTCACCCCA+TGG 0.664932 7_2:+72230546 None:intergenic
TAGAAAGGGCATTCACCATG+GGG 0.664984 7_2:-72230561 Msa1074410:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATTGATATTGGAATTT+AGG - chr7_2:72230870-72230889 Msa1074410:three_prime_UTR 15.0%
!! AGATTTAATATCATAGATTT+CGG - chr7_2:72231872-72231891 Msa1074410:intron 15.0%
!! AATGTCTTTATCTATTAAAA+TGG - chr7_2:72232062-72232081 Msa1074410:intron 15.0%
!! TAATTTCATATTCTTTCTTT+AGG - chr7_2:72232287-72232306 Msa1074410:intron 15.0%
!!! TTTATTGGTTTTGAATTAAT+TGG - chr7_2:72232466-72232485 Msa1074410:intron 15.0%
!! TTTAATTGATATTGGAATTT+AGG - chr7_2:72230870-72230889 Msa1074410:three_prime_UTR 15.0%
!! AGATTTAATATCATAGATTT+CGG - chr7_2:72231872-72231891 Msa1074410:intron 15.0%
!! AATGTCTTTATCTATTAAAA+TGG - chr7_2:72232062-72232081 Msa1074410:intron 15.0%
!! TAATTTCATATTCTTTCTTT+AGG - chr7_2:72232287-72232306 Msa1074410:intron 15.0%
!!! TTTATTGGTTTTGAATTAAT+TGG - chr7_2:72232466-72232485 Msa1074410:intron 15.0%
!! AATATCTAACTCTGTTAATT+CGG - chr7_2:72230690-72230709 Msa1074410:three_prime_UTR 20.0%
!! TTATAACGAACATTAAGAAA+TGG + chr7_2:72230784-72230803 None:intergenic 20.0%
!! ACTATGTGTTTAATTGATAT+TGG - chr7_2:72230862-72230881 Msa1074410:three_prime_UTR 20.0%
!!! TTTTTTTCACTTACTTTGTA+GGG - chr7_2:72231087-72231106 Msa1074410:CDS 20.0%
!! AAAAACATTTCACAATTATG+CGG - chr7_2:72231342-72231361 Msa1074410:intron 20.0%
!!! TTGTTGTGTTTTTAGTTTTT+AGG - chr7_2:72231821-72231840 Msa1074410:intron 20.0%
!! TAACAAAAAAAACATTCACA+TGG + chr7_2:72232581-72232600 None:intergenic 20.0%
!! ATAGAACTTCAAAATAACAT+TGG + chr7_2:72232729-72232748 None:intergenic 20.0%
!! AATATCTAACTCTGTTAATT+CGG - chr7_2:72230690-72230709 Msa1074410:three_prime_UTR 20.0%
!! TTATAACGAACATTAAGAAA+TGG + chr7_2:72230784-72230803 None:intergenic 20.0%
!! ACTATGTGTTTAATTGATAT+TGG - chr7_2:72230862-72230881 Msa1074410:three_prime_UTR 20.0%
!!! TTTTTTTCACTTACTTTGTA+GGG - chr7_2:72231087-72231106 Msa1074410:CDS 20.0%
!! AAAAACATTTCACAATTATG+CGG - chr7_2:72231342-72231361 Msa1074410:intron 20.0%
!!! TTGTTGTGTTTTTAGTTTTT+AGG - chr7_2:72231821-72231840 Msa1074410:intron 20.0%
!! TAACAAAAAAAACATTCACA+TGG + chr7_2:72232581-72232600 None:intergenic 20.0%
!! ATAGAACTTCAAAATAACAT+TGG + chr7_2:72232729-72232748 None:intergenic 20.0%
! ATTTCACAAAAATCAACGAA+GGG + chr7_2:72230936-72230955 None:intergenic 25.0%
! AATTTCACAAAAATCAACGA+AGG + chr7_2:72230937-72230956 None:intergenic 25.0%
!!! GTTTTTTTCACTTACTTTGT+AGG - chr7_2:72231086-72231105 Msa1074410:CDS 25.0%
! AAAACATTTCACAATTATGC+GGG - chr7_2:72231343-72231362 Msa1074410:intron 25.0%
! TTAGTTAATGTTTCAGTTTC+TGG - chr7_2:72231370-72231389 Msa1074410:intron 25.0%
! TTGCTACATGCAATATATTT+AGG - chr7_2:72231409-72231428 Msa1074410:intron 25.0%
! TGCTACATGCAATATATTTA+GGG - chr7_2:72231410-72231429 Msa1074410:intron 25.0%
! TTTGGATATAGTGTGTTAAA+TGG - chr7_2:72231491-72231510 Msa1074410:intron 25.0%
! TTCAATGTAATCATATGTGT+TGG - chr7_2:72231731-72231750 Msa1074410:intron 25.0%
! ATATTAAATCTACGTACTAG+CGG + chr7_2:72231863-72231882 None:intergenic 25.0%
! TGTTATGTTCATGAGTTAAT+AGG - chr7_2:72231896-72231915 Msa1074410:intron 25.0%
!!! TTTATCATTTGTTTTCCGTA+TGG - chr7_2:72232014-72232033 Msa1074410:intron 25.0%
! TAGATAAAGACATTGACAAA+AGG + chr7_2:72232056-72232075 None:intergenic 25.0%
!!! AGTTTAAGTTTTCATCATGT+CGG - chr7_2:72232114-72232133 Msa1074410:intron 25.0%
! AATTCCAAATGATCCAATTA+AGG + chr7_2:72232158-72232177 None:intergenic 25.0%
! GTTTCAGACAAAAAGATATT+TGG - chr7_2:72232199-72232218 Msa1074410:intron 25.0%
!! GTCTTAATTTGGTTGTTTAT+TGG - chr7_2:72232451-72232470 Msa1074410:intron 25.0%
! ATAAAATGTTGAAGCAATCT+TGG - chr7_2:72232603-72232622 Msa1074410:intron 25.0%
! AGCAAAGAAAATCAATGTAA+AGG + chr7_2:72232790-72232809 None:intergenic 25.0%
! AAGACAAACATCATTTAGAA+AGG - chr7_2:72232827-72232846 Msa1074410:five_prime_UTR 25.0%
! AGACAAACATCATTTAGAAA+GGG - chr7_2:72232828-72232847 Msa1074410:five_prime_UTR 25.0%
! ATTTCACAAAAATCAACGAA+GGG + chr7_2:72230936-72230955 None:intergenic 25.0%
! AATTTCACAAAAATCAACGA+AGG + chr7_2:72230937-72230956 None:intergenic 25.0%
!!! GTTTTTTTCACTTACTTTGT+AGG - chr7_2:72231086-72231105 Msa1074410:CDS 25.0%
! AAAACATTTCACAATTATGC+GGG - chr7_2:72231343-72231362 Msa1074410:intron 25.0%
! TTAGTTAATGTTTCAGTTTC+TGG - chr7_2:72231370-72231389 Msa1074410:intron 25.0%
! TTGCTACATGCAATATATTT+AGG - chr7_2:72231409-72231428 Msa1074410:intron 25.0%
! TGCTACATGCAATATATTTA+GGG - chr7_2:72231410-72231429 Msa1074410:intron 25.0%
! TTTGGATATAGTGTGTTAAA+TGG - chr7_2:72231491-72231510 Msa1074410:intron 25.0%
! TTCAATGTAATCATATGTGT+TGG - chr7_2:72231731-72231750 Msa1074410:intron 25.0%
! ATATTAAATCTACGTACTAG+CGG + chr7_2:72231863-72231882 None:intergenic 25.0%
! TGTTATGTTCATGAGTTAAT+AGG - chr7_2:72231896-72231915 Msa1074410:intron 25.0%
!!! TTTATCATTTGTTTTCCGTA+TGG - chr7_2:72232014-72232033 Msa1074410:intron 25.0%
! TAGATAAAGACATTGACAAA+AGG + chr7_2:72232056-72232075 None:intergenic 25.0%
!!! AGTTTAAGTTTTCATCATGT+CGG - chr7_2:72232114-72232133 Msa1074410:intron 25.0%
! AATTCCAAATGATCCAATTA+AGG + chr7_2:72232158-72232177 None:intergenic 25.0%
! GTTTCAGACAAAAAGATATT+TGG - chr7_2:72232199-72232218 Msa1074410:intron 25.0%
!! GTCTTAATTTGGTTGTTTAT+TGG - chr7_2:72232451-72232470 Msa1074410:intron 25.0%
! ATAAAATGTTGAAGCAATCT+TGG - chr7_2:72232603-72232622 Msa1074410:intron 25.0%
! AGCAAAGAAAATCAATGTAA+AGG + chr7_2:72232790-72232809 None:intergenic 25.0%
! AAGACAAACATCATTTAGAA+AGG - chr7_2:72232827-72232846 Msa1074410:five_prime_UTR 25.0%
! AGACAAACATCATTTAGAAA+GGG - chr7_2:72232828-72232847 Msa1074410:five_prime_UTR 25.0%
CGATTCTGTTAAGAACTAAT+AGG - chr7_2:72230756-72230775 Msa1074410:three_prime_UTR 30.0%
ACATTTCACAATTATGACAG+TGG - chr7_2:72230963-72230982 Msa1074410:exon 30.0%
AATTGTTCCTATTGCAAGTT+TGG - chr7_2:72231275-72231294 Msa1074410:CDS 30.0%
ATTGTTCCTATTGCAAGTTT+GGG - chr7_2:72231276-72231295 Msa1074410:CDS 30.0%
TGAAACAGCAATAATTAGAG+AGG + chr7_2:72231304-72231323 None:intergenic 30.0%
GCTACATGCAATATATTTAG+GGG - chr7_2:72231411-72231430 Msa1074410:intron 30.0%
! AATGAAGGAAAGTGTAAAAG+GGG + chr7_2:72231546-72231565 None:intergenic 30.0%
! GAATGAAGGAAAGTGTAAAA+GGG + chr7_2:72231547-72231566 None:intergenic 30.0%
! TGAATGAAGGAAAGTGTAAA+AGG + chr7_2:72231548-72231567 None:intergenic 30.0%
TGACAATATGAACAAACCAT+GGG + chr7_2:72231574-72231593 None:intergenic 30.0%
ATGACAATATGAACAAACCA+TGG + chr7_2:72231575-72231594 None:intergenic 30.0%
TCATGAGTTAATAGGATTAG+AGG - chr7_2:72231904-72231923 Msa1074410:intron 30.0%
AATTGTGACATCAACAATCA+GGG + chr7_2:72231984-72232003 None:intergenic 30.0%
!! GTTTAAGTTTTCATCATGTC+GGG - chr7_2:72232115-72232134 Msa1074410:intron 30.0%
TATATCGTAGCTTCCTTAAT+TGG - chr7_2:72232142-72232161 Msa1074410:intron 30.0%
TCAGACAAAAAGATATTTGG+AGG - chr7_2:72232202-72232221 Msa1074410:intron 30.0%
GGTAAGTTATGCTTCTTTAT+AGG - chr7_2:72232223-72232242 Msa1074410:intron 30.0%
ATGAAATTCTGGTGACTAAT+AGG + chr7_2:72232413-72232432 None:intergenic 30.0%
AACAACCAAATTAAGACTCA+GGG + chr7_2:72232448-72232467 None:intergenic 30.0%
AAACAACCAAATTAAGACTC+AGG + chr7_2:72232449-72232468 None:intergenic 30.0%
! TTGAATTAATTGGTTCACCT+TGG - chr7_2:72232476-72232495 Msa1074410:intron 30.0%
TTGCAGTTCAATTACTGTTT+GGG - chr7_2:72232547-72232566 Msa1074410:intron 30.0%
TAAGATTAGCAGAGTTGTTT+AGG - chr7_2:72232753-72232772 Msa1074410:intron 30.0%
AAGATTAGCAGAGTTGTTTA+GGG - chr7_2:72232754-72232773 Msa1074410:intron 30.0%
TTGTCTTGAAAAAACTGGTA+TGG + chr7_2:72232814-72232833 None:intergenic 30.0%
GATGTTTGTCTTGAAAAAAC+TGG + chr7_2:72232819-72232838 None:intergenic 30.0%
CGATTCTGTTAAGAACTAAT+AGG - chr7_2:72230756-72230775 Msa1074410:three_prime_UTR 30.0%
ACATTTCACAATTATGACAG+TGG - chr7_2:72230963-72230982 Msa1074410:exon 30.0%
AATTGTTCCTATTGCAAGTT+TGG - chr7_2:72231275-72231294 Msa1074410:CDS 30.0%
ATTGTTCCTATTGCAAGTTT+GGG - chr7_2:72231276-72231295 Msa1074410:CDS 30.0%
TGAAACAGCAATAATTAGAG+AGG + chr7_2:72231304-72231323 None:intergenic 30.0%
GCTACATGCAATATATTTAG+GGG - chr7_2:72231411-72231430 Msa1074410:intron 30.0%
! AATGAAGGAAAGTGTAAAAG+GGG + chr7_2:72231546-72231565 None:intergenic 30.0%
! GAATGAAGGAAAGTGTAAAA+GGG + chr7_2:72231547-72231566 None:intergenic 30.0%
! TGAATGAAGGAAAGTGTAAA+AGG + chr7_2:72231548-72231567 None:intergenic 30.0%
TGACAATATGAACAAACCAT+GGG + chr7_2:72231574-72231593 None:intergenic 30.0%
ATGACAATATGAACAAACCA+TGG + chr7_2:72231575-72231594 None:intergenic 30.0%
TCATGAGTTAATAGGATTAG+AGG - chr7_2:72231904-72231923 Msa1074410:intron 30.0%
AATTGTGACATCAACAATCA+GGG + chr7_2:72231984-72232003 None:intergenic 30.0%
!! GTTTAAGTTTTCATCATGTC+GGG - chr7_2:72232115-72232134 Msa1074410:intron 30.0%
TATATCGTAGCTTCCTTAAT+TGG - chr7_2:72232142-72232161 Msa1074410:intron 30.0%
TCAGACAAAAAGATATTTGG+AGG - chr7_2:72232202-72232221 Msa1074410:intron 30.0%
GGTAAGTTATGCTTCTTTAT+AGG - chr7_2:72232223-72232242 Msa1074410:intron 30.0%
ATGAAATTCTGGTGACTAAT+AGG + chr7_2:72232413-72232432 None:intergenic 30.0%
AACAACCAAATTAAGACTCA+GGG + chr7_2:72232448-72232467 None:intergenic 30.0%
AAACAACCAAATTAAGACTC+AGG + chr7_2:72232449-72232468 None:intergenic 30.0%
! TTGAATTAATTGGTTCACCT+TGG - chr7_2:72232476-72232495 Msa1074410:intron 30.0%
TTGCAGTTCAATTACTGTTT+GGG - chr7_2:72232547-72232566 Msa1074410:intron 30.0%
TAAGATTAGCAGAGTTGTTT+AGG - chr7_2:72232753-72232772 Msa1074410:intron 30.0%
AAGATTAGCAGAGTTGTTTA+GGG - chr7_2:72232754-72232773 Msa1074410:intron 30.0%
TTGTCTTGAAAAAACTGGTA+TGG + chr7_2:72232814-72232833 None:intergenic 30.0%
GATGTTTGTCTTGAAAAAAC+TGG + chr7_2:72232819-72232838 None:intergenic 30.0%
TTTCACAATTATGACAGTGG+AGG - chr7_2:72230966-72230985 Msa1074410:exon 35.0%
AACAGTACGCTAGATACAAA+AGG + chr7_2:72231189-72231208 None:intergenic 35.0%
! TTTACTGGTGTGTGACTTTT+AGG - chr7_2:72231242-72231261 Msa1074410:CDS 35.0%
! TTACTGGTGTGTGACTTTTA+GGG - chr7_2:72231243-72231262 Msa1074410:CDS 35.0%
TATCCAAAATGTTAGCAGCA+GGG + chr7_2:72231479-72231498 None:intergenic 35.0%
ATATCCAAAATGTTAGCAGC+AGG + chr7_2:72231480-72231499 None:intergenic 35.0%
TTTCCTTCATTCAAATCCCA+TGG - chr7_2:72231555-72231574 Msa1074410:intron 35.0%
AAACCATGGGATTTGAATGA+AGG + chr7_2:72231561-72231580 None:intergenic 35.0%
ATAGATTCCAACACACACAA+TGG + chr7_2:72231654-72231673 None:intergenic 35.0%
TATGAACCACATATACTCCT+CGG + chr7_2:72231786-72231805 None:intergenic 35.0%
AGTATATGTGGTTCATAGGT+TGG - chr7_2:72231789-72231808 Msa1074410:intron 35.0%
CAATTGTGACATCAACAATC+AGG + chr7_2:72231985-72232004 None:intergenic 35.0%
!! ATTTGTTTTCCGTATGGAGA+TGG - chr7_2:72232020-72232039 Msa1074410:intron 35.0%
GAGAAATATCCATCTCCATA+CGG + chr7_2:72232032-72232051 None:intergenic 35.0%
GCTTCCTTAATTGGATCATT+TGG - chr7_2:72232151-72232170 Msa1074410:intron 35.0%
! TTGGATCATTTGGAATTGCA+TGG - chr7_2:72232161-72232180 Msa1074410:intron 35.0%
CTGACAAGAAGTTTAATGCA+TGG - chr7_2:72232354-72232373 Msa1074410:intron 35.0%
GAGACTTCACAATGAAATTC+TGG + chr7_2:72232424-72232443 None:intergenic 35.0%
CTTGCAGTTCAATTACTGTT+TGG - chr7_2:72232546-72232565 Msa1074410:intron 35.0%
TGCAGTTCAATTACTGTTTG+GGG - chr7_2:72232548-72232567 Msa1074410:intron 35.0%
AGCTGAAACAATGACTGTAA+AGG + chr7_2:72232705-72232724 None:intergenic 35.0%
TTTCACAATTATGACAGTGG+AGG - chr7_2:72230966-72230985 Msa1074410:exon 35.0%
AACAGTACGCTAGATACAAA+AGG + chr7_2:72231189-72231208 None:intergenic 35.0%
! TTTACTGGTGTGTGACTTTT+AGG - chr7_2:72231242-72231261 Msa1074410:CDS 35.0%
! TTACTGGTGTGTGACTTTTA+GGG - chr7_2:72231243-72231262 Msa1074410:CDS 35.0%
TATCCAAAATGTTAGCAGCA+GGG + chr7_2:72231479-72231498 None:intergenic 35.0%
ATATCCAAAATGTTAGCAGC+AGG + chr7_2:72231480-72231499 None:intergenic 35.0%
TTTCCTTCATTCAAATCCCA+TGG - chr7_2:72231555-72231574 Msa1074410:intron 35.0%
AAACCATGGGATTTGAATGA+AGG + chr7_2:72231561-72231580 None:intergenic 35.0%
ATAGATTCCAACACACACAA+TGG + chr7_2:72231654-72231673 None:intergenic 35.0%
TATGAACCACATATACTCCT+CGG + chr7_2:72231786-72231805 None:intergenic 35.0%
AGTATATGTGGTTCATAGGT+TGG - chr7_2:72231789-72231808 Msa1074410:intron 35.0%
CAATTGTGACATCAACAATC+AGG + chr7_2:72231985-72232004 None:intergenic 35.0%
!! ATTTGTTTTCCGTATGGAGA+TGG - chr7_2:72232020-72232039 Msa1074410:intron 35.0%
GAGAAATATCCATCTCCATA+CGG + chr7_2:72232032-72232051 None:intergenic 35.0%
GCTTCCTTAATTGGATCATT+TGG - chr7_2:72232151-72232170 Msa1074410:intron 35.0%
! TTGGATCATTTGGAATTGCA+TGG - chr7_2:72232161-72232180 Msa1074410:intron 35.0%
CTGACAAGAAGTTTAATGCA+TGG - chr7_2:72232354-72232373 Msa1074410:intron 35.0%
GAGACTTCACAATGAAATTC+TGG + chr7_2:72232424-72232443 None:intergenic 35.0%
CTTGCAGTTCAATTACTGTT+TGG - chr7_2:72232546-72232565 Msa1074410:intron 35.0%
TGCAGTTCAATTACTGTTTG+GGG - chr7_2:72232548-72232567 Msa1074410:intron 35.0%
AGCTGAAACAATGACTGTAA+AGG + chr7_2:72232705-72232724 None:intergenic 35.0%
ACAAACAGATCTCAAGCTTC+AGG - chr7_2:72230613-72230632 Msa1074410:three_prime_UTR 40.0%
AGAGAAGATTGCTAGGGTTA+GGG + chr7_2:72230645-72230664 None:intergenic 40.0%
AGGTTACTCAATTTGTGCGT+TGG - chr7_2:72230890-72230909 Msa1074410:three_prime_UTR 40.0%
!! TGTGAGGGGTTTTGGTAAAT+TGG - chr7_2:72230993-72231012 Msa1074410:CDS 40.0%
!! GTTTTGGTAAATTGGCTGTG+TGG - chr7_2:72231001-72231020 Msa1074410:CDS 40.0%
! ATTGCTCCTTTTCTTTGTGC+TGG - chr7_2:72231126-72231145 Msa1074410:intron 40.0%
TCTAGCGTACTGTTCTTGTT+TGG - chr7_2:72231195-72231214 Msa1074410:intron 40.0%
! CTAGCGTACTGTTCTTGTTT+GGG - chr7_2:72231196-72231215 Msa1074410:intron 40.0%
! TCTTGTTTGGGAGTTGTAAC+AGG - chr7_2:72231208-72231227 Msa1074410:CDS 40.0%
ATCCAAAATGTTAGCAGCAG+GGG + chr7_2:72231478-72231497 None:intergenic 40.0%
GTAAAAGGGGTTTGCAGTTT+GGG + chr7_2:72231533-72231552 None:intergenic 40.0%
TGTAAAAGGGGTTTGCAGTT+TGG + chr7_2:72231534-72231553 None:intergenic 40.0%
TACATAGCCATTGTGTGTGT+TGG - chr7_2:72231644-72231663 Msa1074410:intron 40.0%
GAATCTATGAAGCACAGACA+CGG - chr7_2:72231666-72231685 Msa1074410:intron 40.0%
CACATATACTCCTCGGATTT+AGG + chr7_2:72231779-72231798 None:intergenic 40.0%
! CTAAATCCGAGGAGTATATG+TGG - chr7_2:72231777-72231796 Msa1074410:intron 40.0%
! GAGGAGTATATGTGGTTCAT+AGG - chr7_2:72231785-72231804 Msa1074410:intron 40.0%
! TTGGGTAATGGTCTATGGTT+GGG - chr7_2:72231951-72231970 Msa1074410:intron 40.0%
CTAATAGGATTCATGACGAC+TGG + chr7_2:72232398-72232417 None:intergenic 40.0%
! TAATTGGTTCACCTTGGTCA+TGG - chr7_2:72232482-72232501 Msa1074410:intron 40.0%
GTTGACATATTCCATGACCA+AGG + chr7_2:72232496-72232515 None:intergenic 40.0%
GCAGTTCAATTACTGTTTGG+GGG - chr7_2:72232549-72232568 Msa1074410:intron 40.0%
!! TTTAGAAAGGGCATTCACCA+TGG - chr7_2:72232840-72232859 Msa1074410:five_prime_UTR 40.0%
!! TTAGAAAGGGCATTCACCAT+GGG - chr7_2:72232841-72232860 Msa1074410:five_prime_UTR 40.0%
ACAAACAGATCTCAAGCTTC+AGG - chr7_2:72230613-72230632 Msa1074410:three_prime_UTR 40.0%
AGAGAAGATTGCTAGGGTTA+GGG + chr7_2:72230645-72230664 None:intergenic 40.0%
AGGTTACTCAATTTGTGCGT+TGG - chr7_2:72230890-72230909 Msa1074410:three_prime_UTR 40.0%
!! TGTGAGGGGTTTTGGTAAAT+TGG - chr7_2:72230993-72231012 Msa1074410:CDS 40.0%
!! GTTTTGGTAAATTGGCTGTG+TGG - chr7_2:72231001-72231020 Msa1074410:CDS 40.0%
! ATTGCTCCTTTTCTTTGTGC+TGG - chr7_2:72231126-72231145 Msa1074410:intron 40.0%
TCTAGCGTACTGTTCTTGTT+TGG - chr7_2:72231195-72231214 Msa1074410:intron 40.0%
! CTAGCGTACTGTTCTTGTTT+GGG - chr7_2:72231196-72231215 Msa1074410:intron 40.0%
! TCTTGTTTGGGAGTTGTAAC+AGG - chr7_2:72231208-72231227 Msa1074410:CDS 40.0%
ATCCAAAATGTTAGCAGCAG+GGG + chr7_2:72231478-72231497 None:intergenic 40.0%
GTAAAAGGGGTTTGCAGTTT+GGG + chr7_2:72231533-72231552 None:intergenic 40.0%
TGTAAAAGGGGTTTGCAGTT+TGG + chr7_2:72231534-72231553 None:intergenic 40.0%
TACATAGCCATTGTGTGTGT+TGG - chr7_2:72231644-72231663 Msa1074410:intron 40.0%
GAATCTATGAAGCACAGACA+CGG - chr7_2:72231666-72231685 Msa1074410:intron 40.0%
CACATATACTCCTCGGATTT+AGG + chr7_2:72231779-72231798 None:intergenic 40.0%
! CTAAATCCGAGGAGTATATG+TGG - chr7_2:72231777-72231796 Msa1074410:intron 40.0%
! GAGGAGTATATGTGGTTCAT+AGG - chr7_2:72231785-72231804 Msa1074410:intron 40.0%
! TTGGGTAATGGTCTATGGTT+GGG - chr7_2:72231951-72231970 Msa1074410:intron 40.0%
CTAATAGGATTCATGACGAC+TGG + chr7_2:72232398-72232417 None:intergenic 40.0%
! TAATTGGTTCACCTTGGTCA+TGG - chr7_2:72232482-72232501 Msa1074410:intron 40.0%
GTTGACATATTCCATGACCA+AGG + chr7_2:72232496-72232515 None:intergenic 40.0%
GCAGTTCAATTACTGTTTGG+GGG - chr7_2:72232549-72232568 Msa1074410:intron 40.0%
!! TTTAGAAAGGGCATTCACCA+TGG - chr7_2:72232840-72232859 Msa1074410:five_prime_UTR 40.0%
!! TTAGAAAGGGCATTCACCAT+GGG - chr7_2:72232841-72232860 Msa1074410:five_prime_UTR 40.0%
GAGAGAAGATTGCTAGGGTT+AGG + chr7_2:72230646-72230665 None:intergenic 45.0%
GCGTAGAGAGAAGATTGCTA+GGG + chr7_2:72230651-72230670 None:intergenic 45.0%
TGCGTAGAGAGAAGATTGCT+AGG + chr7_2:72230652-72230671 None:intergenic 45.0%
! TGTGGCGCAGCGTATTTTAT+AGG - chr7_2:72231019-72231038 Msa1074410:CDS 45.0%
! GTGGCGCAGCGTATTTTATA+GGG - chr7_2:72231020-72231039 Msa1074410:CDS 45.0%
! CGCAGCGTATTTTATAGGGA+GGG - chr7_2:72231024-72231043 Msa1074410:CDS 45.0%
! ATTTTATAGGGAGGGTCTGC+TGG - chr7_2:72231032-72231051 Msa1074410:CDS 45.0%
TGTATCAGCGAAAGGATGAG+AGG + chr7_2:72231152-72231171 None:intergenic 45.0%
CTCATCCTTTCGCTGATACA+AGG - chr7_2:72231152-72231171 Msa1074410:intron 45.0%
! CAGGTGCTCTTGTGTTTTAC+TGG - chr7_2:72231227-72231246 Msa1074410:CDS 45.0%
!! CATATGTGTTGGTGTCGTCT+TGG - chr7_2:72231742-72231761 Msa1074410:intron 45.0%
GTTGGATAAGCCTAAATCCG+AGG - chr7_2:72231766-72231785 Msa1074410:intron 45.0%
! GTTGGGTAATGGTCTATGGT+TGG - chr7_2:72231950-72231969 Msa1074410:intron 45.0%
ATAGGATTCATGACGACTGG+AGG + chr7_2:72232395-72232414 None:intergenic 45.0%
TCTCGCCCTGAGTCTTAATT+TGG - chr7_2:72232440-72232459 Msa1074410:intron 45.0%
!! TAGAAAGGGCATTCACCATG+GGG - chr7_2:72232842-72232861 Msa1074410:five_prime_UTR 45.0%
GAGAGAAGATTGCTAGGGTT+AGG + chr7_2:72230646-72230665 None:intergenic 45.0%
GCGTAGAGAGAAGATTGCTA+GGG + chr7_2:72230651-72230670 None:intergenic 45.0%
TGCGTAGAGAGAAGATTGCT+AGG + chr7_2:72230652-72230671 None:intergenic 45.0%
! TGTGGCGCAGCGTATTTTAT+AGG - chr7_2:72231019-72231038 Msa1074410:CDS 45.0%
! GTGGCGCAGCGTATTTTATA+GGG - chr7_2:72231020-72231039 Msa1074410:CDS 45.0%
! CGCAGCGTATTTTATAGGGA+GGG - chr7_2:72231024-72231043 Msa1074410:CDS 45.0%
! ATTTTATAGGGAGGGTCTGC+TGG - chr7_2:72231032-72231051 Msa1074410:CDS 45.0%
TGTATCAGCGAAAGGATGAG+AGG + chr7_2:72231152-72231171 None:intergenic 45.0%
CTCATCCTTTCGCTGATACA+AGG - chr7_2:72231152-72231171 Msa1074410:intron 45.0%
! CAGGTGCTCTTGTGTTTTAC+TGG - chr7_2:72231227-72231246 Msa1074410:CDS 45.0%
!! CATATGTGTTGGTGTCGTCT+TGG - chr7_2:72231742-72231761 Msa1074410:intron 45.0%
GTTGGATAAGCCTAAATCCG+AGG - chr7_2:72231766-72231785 Msa1074410:intron 45.0%
! GTTGGGTAATGGTCTATGGT+TGG - chr7_2:72231950-72231969 Msa1074410:intron 45.0%
ATAGGATTCATGACGACTGG+AGG + chr7_2:72232395-72232414 None:intergenic 45.0%
TCTCGCCCTGAGTCTTAATT+TGG - chr7_2:72232440-72232459 Msa1074410:intron 45.0%
!! TAGAAAGGGCATTCACCATG+GGG - chr7_2:72232842-72232861 Msa1074410:five_prime_UTR 45.0%
! GCGCAGCGTATTTTATAGGG+AGG - chr7_2:72231023-72231042 Msa1074410:CDS 50.0%
!!! GGAGGGTCTGCTGGTTTTTT+AGG - chr7_2:72231041-72231060 Msa1074410:CDS 50.0%
GAGAGGCCAGCACAAAGAAA+AGG + chr7_2:72231135-72231154 None:intergenic 50.0%
CACGTCCTTGTATCAGCGAA+AGG + chr7_2:72231160-72231179 None:intergenic 50.0%
! GAGGCACCCAAACTTGCAAT+AGG + chr7_2:72231285-72231304 None:intergenic 50.0%
AAATGTTAGCAGCAGGGGCA+GGG + chr7_2:72231473-72231492 None:intergenic 50.0%
AAAATGTTAGCAGCAGGGGC+AGG + chr7_2:72231474-72231493 None:intergenic 50.0%
! TGCCCCTGCTGCTAACATTT+TGG - chr7_2:72231473-72231492 Msa1074410:intron 50.0%
!! TGTTGGTGTCGTCTTGGTGT+TGG - chr7_2:72231748-72231767 Msa1074410:intron 50.0%
!!! ATCTTGTTTTCCTGCCACGC+TGG - chr7_2:72231928-72231947 Msa1074410:intron 50.0%
! GCTGGTTGGGTAATGGTCTA+TGG - chr7_2:72231946-72231965 Msa1074410:intron 50.0%
TCAGTCTCTTCTTGCCATGC+CGG + chr7_2:72232338-72232357 None:intergenic 50.0%
! GCGCAGCGTATTTTATAGGG+AGG - chr7_2:72231023-72231042 Msa1074410:CDS 50.0%
!!! GGAGGGTCTGCTGGTTTTTT+AGG - chr7_2:72231041-72231060 Msa1074410:CDS 50.0%
GAGAGGCCAGCACAAAGAAA+AGG + chr7_2:72231135-72231154 None:intergenic 50.0%
CACGTCCTTGTATCAGCGAA+AGG + chr7_2:72231160-72231179 None:intergenic 50.0%
! GAGGCACCCAAACTTGCAAT+AGG + chr7_2:72231285-72231304 None:intergenic 50.0%
AAATGTTAGCAGCAGGGGCA+GGG + chr7_2:72231473-72231492 None:intergenic 50.0%
AAAATGTTAGCAGCAGGGGC+AGG + chr7_2:72231474-72231493 None:intergenic 50.0%
! TGCCCCTGCTGCTAACATTT+TGG - chr7_2:72231473-72231492 Msa1074410:intron 50.0%
!! TGTTGGTGTCGTCTTGGTGT+TGG - chr7_2:72231748-72231767 Msa1074410:intron 50.0%
!!! ATCTTGTTTTCCTGCCACGC+TGG - chr7_2:72231928-72231947 Msa1074410:intron 50.0%
! GCTGGTTGGGTAATGGTCTA+TGG - chr7_2:72231946-72231965 Msa1074410:intron 50.0%
TCAGTCTCTTCTTGCCATGC+CGG + chr7_2:72232338-72232357 None:intergenic 50.0%
TGACAGTGGAGGCTGTTGTG+AGG - chr7_2:72230977-72230996 Msa1074410:CDS 55.0%
GACAGTGGAGGCTGTTGTGA+GGG - chr7_2:72230978-72230997 Msa1074410:CDS 55.0%
ACAGTGGAGGCTGTTGTGAG+GGG - chr7_2:72230979-72230998 Msa1074410:CDS 55.0%
! GAGGCTGTTGTGAGGGGTTT+TGG - chr7_2:72230985-72231004 Msa1074410:CDS 55.0%
TGAAGCACAGACACGGACAC+TGG - chr7_2:72231673-72231692 Msa1074410:intron 55.0%
! TGTTTTCCTGCCACGCTGGT+TGG - chr7_2:72231932-72231951 Msa1074410:intron 55.0%
!! GTTTTCCTGCCACGCTGGTT+GGG - chr7_2:72231933-72231952 Msa1074410:intron 55.0%
AGACCATTACCCAACCAGCG+TGG + chr7_2:72231945-72231964 None:intergenic 55.0%
CTGTGCCACAGCTTCAAACC+CGG - chr7_2:72232316-72232335 Msa1074410:intron 55.0%
CAGTCTCTTCTTGCCATGCC+GGG + chr7_2:72232337-72232356 None:intergenic 55.0%
GCTACGAGCAGCACGAATCT+TGG - chr7_2:72232662-72232681 Msa1074410:intron 55.0%
! TTTTCTGCTCCGAGAGAGCC+AGG + chr7_2:72230555-72230574 None:intergenic 55.0%
TGACAGTGGAGGCTGTTGTG+AGG - chr7_2:72230977-72230996 Msa1074410:CDS 55.0%
GACAGTGGAGGCTGTTGTGA+GGG - chr7_2:72230978-72230997 Msa1074410:CDS 55.0%
ACAGTGGAGGCTGTTGTGAG+GGG - chr7_2:72230979-72230998 Msa1074410:CDS 55.0%
! GAGGCTGTTGTGAGGGGTTT+TGG - chr7_2:72230985-72231004 Msa1074410:CDS 55.0%
TGAAGCACAGACACGGACAC+TGG - chr7_2:72231673-72231692 Msa1074410:intron 55.0%
! TGTTTTCCTGCCACGCTGGT+TGG - chr7_2:72231932-72231951 Msa1074410:intron 55.0%
!! GTTTTCCTGCCACGCTGGTT+GGG - chr7_2:72231933-72231952 Msa1074410:intron 55.0%
AGACCATTACCCAACCAGCG+TGG + chr7_2:72231945-72231964 None:intergenic 55.0%
CTGTGCCACAGCTTCAAACC+CGG - chr7_2:72232316-72232335 Msa1074410:intron 55.0%
CAGTCTCTTCTTGCCATGCC+GGG + chr7_2:72232337-72232356 None:intergenic 55.0%
GCTACGAGCAGCACGAATCT+TGG - chr7_2:72232662-72232681 Msa1074410:intron 55.0%
CATTACCCAACCAGCGTGGC+AGG + chr7_2:72231941-72231960 None:intergenic 60.0%
! CTGCCACGCTGGTTGGGTAA+TGG - chr7_2:72231939-72231958 Msa1074410:intron 60.0%
! CCATGCCGGGTTTGAAGCTG+TGG + chr7_2:72232324-72232343 None:intergenic 60.0%
CCACAGCTTCAAACCCGGCA+TGG - chr7_2:72232321-72232340 Msa1074410:intron 60.0%
AATGCATGGCCTCGCACTGC+TGG - chr7_2:72232368-72232387 Msa1074410:intron 60.0%
CATTACCCAACCAGCGTGGC+AGG + chr7_2:72231941-72231960 None:intergenic 60.0%
! CTGCCACGCTGGTTGGGTAA+TGG - chr7_2:72231939-72231958 Msa1074410:intron 60.0%
! CCATGCCGGGTTTGAAGCTG+TGG + chr7_2:72232324-72232343 None:intergenic 60.0%
CCACAGCTTCAAACCCGGCA+TGG - chr7_2:72232321-72232340 Msa1074410:intron 60.0%
AATGCATGGCCTCGCACTGC+TGG - chr7_2:72232368-72232387 Msa1074410:intron 60.0%
ACTGGAGGACCAGCAGTGCG+AGG + chr7_2:72232380-72232399 None:intergenic 65.0%
ACTGGAGGACCAGCAGTGCG+AGG + chr7_2:72232380-72232399 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 72230553 72232872 72230553 ID=Msa1074410;Name=Msa1074410
chr7_2 mRNA 72230553 72232872 72230553 ID=Msa1074410-mRNA-1;Parent=Msa1074410;Name=Msa1074410-mRNA-1;_AED=0.01;_eAED=0.01;_QI=96|1|1|1|0.5|0.33|3|415|80
chr7_2 exon 72230553 72231116 72230553 ID=Msa1074410-mRNA-1:exon:16580;Parent=Msa1074410-mRNA-1
chr7_2 exon 72231202 72231309 72231202 ID=Msa1074410-mRNA-1:exon:16579;Parent=Msa1074410-mRNA-1
chr7_2 exon 72232791 72232872 72232791 ID=Msa1074410-mRNA-1:exon:16578;Parent=Msa1074410-mRNA-1
chr7_2 five_prime_UTR 72232791 72232872 72232791 ID=Msa1074410-mRNA-1:five_prime_utr;Parent=Msa1074410-mRNA-1
chr7_2 five_prime_UTR 72231296 72231309 72231296 ID=Msa1074410-mRNA-1:five_prime_utr;Parent=Msa1074410-mRNA-1
chr7_2 CDS 72231202 72231295 72231202 ID=Msa1074410-mRNA-1:cds;Parent=Msa1074410-mRNA-1
chr7_2 CDS 72230968 72231116 72230968 ID=Msa1074410-mRNA-1:cds;Parent=Msa1074410-mRNA-1
chr7_2 three_prime_UTR 72230553 72230967 72230553 ID=Msa1074410-mRNA-1:three_prime_utr;Parent=Msa1074410-mRNA-1
Gene Sequence

>Msa1074410

ATGTCGGGCAAGTATATCGTAGCTTCCTTAATTGGATCATTTGGAATTGCATGGATCTGTGATTATTATGTTTCAGACAAAAAGATATTTGGAGGTACTGTCTGTGCCACAGCTTCAAACCCGGCATGGCAAGAAGAGACTGACAAGAAGTTTAATGCATGGCCTCGCACTGCTGGTCCTCCAGTCGTCATGAATCCTATTAGTCACCAGAATTTCATTGTGAAGTCTCGCCCTGAGTCTTAA
Protein sequence

>Msa1074410

MSGKYIVASLIGSFGIAWICDYYVSDKKIFGGTVCATASNPAWQEETDKKFNAWPRTAGPPVVMNPISHQNFIVKSRPES*