Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077100 | XP_013449901.1 | 91.102 | 1225 | 107 | 2 | 1 | 1224 | 1 | 1224 | 0.0 | 2154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077100 | sp|Q8VZG8|MIK2_ARATH | 42.430 | 971 | 541 | 12 | 239 | 1200 | 84 | 1045 | 0.0 | 741 |
| Msa1077100 | sp|Q8VZG8|MIK2_ARATH | 33.037 | 675 | 395 | 12 | 1 | 648 | 19 | 663 | 1.51e-80 | 290 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077100 | A0A072U2L6 | 91.102 | 1225 | 107 | 2 | 1 | 1224 | 1 | 1224 | 0.0 | 2154 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1036770 | Msa1077100 | 0.853519 | 2.264943e-61 | -8.615850e-47 |
| Msa1077100 | Msa1163840 | 0.833541 | 5.027182e-56 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077100 | MtrunA17_Chr7g0261331 | 91.102 | 1225 | 107 | 2 | 1 | 1224 | 1 | 1224 | 0.0 | 2154 |
| Msa1077100 | MtrunA17_Chr7g0261901 | 87.053 | 587 | 75 | 1 | 324 | 910 | 4 | 589 | 0.0 | 990 |
| Msa1077100 | MtrunA17_Chr7g0261901 | 32.064 | 499 | 315 | 2 | 198 | 696 | 23 | 497 | 8.05e-57 | 208 |
| Msa1077100 | MtrunA17_Chr7g0261901 | 33.680 | 481 | 288 | 7 | 100 | 553 | 22 | 498 | 4.80e-54 | 199 |
| Msa1077100 | MtrunA17_Chr7g0261901 | 32.530 | 498 | 307 | 4 | 125 | 597 | 1 | 494 | 5.47e-54 | 199 |
| Msa1077100 | MtrunA17_Chr7g0261901 | 31.265 | 419 | 260 | 5 | 86 | 503 | 106 | 497 | 2.17e-46 | 177 |
| Msa1077100 | MtrunA17_Chr7g0261901 | 29.915 | 234 | 160 | 4 | 79 | 311 | 268 | 498 | 5.94e-19 | 92.4 |
| Msa1077100 | MtrunA17_Chr1g0165901 | 42.272 | 1197 | 648 | 15 | 10 | 1199 | 79 | 1239 | 0.0 | 845 |
| Msa1077100 | MtrunA17_Chr1g0165211 | 41.319 | 1198 | 658 | 14 | 10 | 1199 | 11 | 1171 | 0.0 | 845 |
| Msa1077100 | MtrunA17_Chr7g0232131 | 42.177 | 1176 | 635 | 15 | 31 | 1199 | 43 | 1180 | 0.0 | 803 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077100 | AT4G08850.1 | 42.430 | 971 | 541 | 12 | 239 | 1200 | 84 | 1045 | 0.0 | 741 |
| Msa1077100 | AT4G08850.1 | 33.037 | 675 | 395 | 12 | 1 | 648 | 19 | 663 | 1.54e-81 | 290 |
| Msa1077100 | AT1G35710.1 | 39.443 | 1042 | 563 | 15 | 211 | 1200 | 95 | 1120 | 0.0 | 721 |
| Msa1077100 | AT5G44700.1 | 35.581 | 1231 | 708 | 25 | 29 | 1199 | 27 | 1232 | 0.0 | 660 |
| Msa1077100 | AT4G20140.1 | 35.294 | 1224 | 723 | 26 | 31 | 1199 | 26 | 1235 | 0.0 | 659 |
| Msa1077100 | AT4G08850.2 | 42.153 | 873 | 489 | 11 | 239 | 1102 | 84 | 949 | 0.0 | 647 |
| Msa1077100 | AT4G08850.2 | 32.296 | 675 | 424 | 9 | 1 | 672 | 19 | 663 | 1.42e-81 | 289 |
| Msa1077100 | AT5G63930.2 | 37.476 | 1022 | 612 | 14 | 188 | 1196 | 4 | 1011 | 0.0 | 620 |
| Msa1077100 | AT5G63930.2 | 34.604 | 656 | 397 | 7 | 68 | 720 | 1 | 627 | 1.73e-87 | 307 |
| Msa1077100 | AT5G63930.2 | 32.895 | 456 | 273 | 10 | 30 | 480 | 201 | 628 | 3.31e-44 | 175 |
Find 262 sgRNAs with CRISPR-Local
Find 299 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACACCCTGGAGAATTCT+TGG | 0.353746 | 7_2:-75652012 | Msa1077100:CDS |
| AAAAGTTTGATCCAAGTTCA+AGG | 0.459368 | 7_2:+75655044 | None:intergenic |
| AAACAAGTCTTTAGCATCAA+TGG | 0.352774 | 7_2:-75651976 | Msa1077100:CDS |
| AAACACCCTGGAGAATTCTT+GGG | 0.507248 | 7_2:-75652011 | Msa1077100:CDS |
| AAAGGCTTCCACCTCCAACA+GGG | 0.688512 | 7_2:-75651926 | Msa1077100:CDS |
| AAAGGTTGGTAAGATTGGTG+AGG | 0.636158 | 7_2:+75655407 | None:intergenic |
| AAATCCACTGATTGAAGGCT+AGG | 0.479716 | 7_2:+75653171 | None:intergenic |
| AAATCTAACTTTCCTAAGCT+TGG | 0.486841 | 7_2:-75654546 | Msa1077100:CDS |
| AAATTACCATAAAAGGATTC+TGG | 0.356220 | 7_2:+75654860 | None:intergenic |
| AACCTCAATAGCAACAGGTT+TGG | 0.485637 | 7_2:-75655256 | Msa1077100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGTTTGATCCAAGTTCA+AGG | + | chr7_2:75652273-75652292 | None:intergenic | 30.0% | |
| TGAATATTGAGACCAATCAA+GGG | + | chr7_2:75652305-75652324 | None:intergenic | 30.0% | |
| TTGAATATTGAGACCAATCA+AGG | + | chr7_2:75652306-75652325 | None:intergenic | 30.0% | |
| ATGCTTTCCTTAAATTACCT+TGG | - | chr7_2:75652328-75652347 | Msa1077100:CDS | 30.0% | |
| TTCCTTAAATTACCTTGGTT+TGG | - | chr7_2:75652333-75652352 | Msa1077100:CDS | 30.0% | |
| TTGGAGGAAAATGAATTTAC+TGG | - | chr7_2:75652352-75652371 | Msa1077100:CDS | 30.0% | |
| AAATCTAACTTTCCTAAGCT+TGG | - | chr7_2:75652768-75652787 | Msa1077100:CDS | 30.0% | |
| AGAAAATAGGTTAACAGGAA+TGG | - | chr7_2:75652939-75652958 | Msa1077100:CDS | 30.0% | |
| TGAAGAGTTAGGAAACTTAA+AGG | - | chr7_2:75653038-75653057 | Msa1077100:CDS | 30.0% | |
| TATGTTTGTGAGATTCCAAA+TGG | + | chr7_2:75653118-75653137 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 75651746 | 75655590 | 75651746 | ID=Msa1077100;Name=Msa1077100 |
| chr7_2 | mRNA | 75651746 | 75655590 | 75651746 | ID=Msa1077100-mRNA-1;Parent=Msa1077100;Name=Msa1077100-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|1224 |
| chr7_2 | exon | 75652284 | 75655590 | 75652284 | ID=Msa1077100-mRNA-1:exon:18075;Parent=Msa1077100-mRNA-1 |
| chr7_2 | exon | 75651746 | 75652113 | 75651746 | ID=Msa1077100-mRNA-1:exon:18074;Parent=Msa1077100-mRNA-1 |
| chr7_2 | CDS | 75652284 | 75655590 | 75652284 | ID=Msa1077100-mRNA-1:cds;Parent=Msa1077100-mRNA-1 |
| chr7_2 | CDS | 75651746 | 75652113 | 75651746 | ID=Msa1077100-mRNA-1:cds;Parent=Msa1077100-mRNA-1 |
| Gene Sequence |
>Msa1077100 ATGGCAACTATTCAAAAGGTTAATGCTCTTGTCTTCTATATACTGCTTATATCTTTGCTTCCATTGAATATCACAGCATCACTAAAAACAGAAGCAGAAGCTCTTGTCAAATGGAAAAACAGTCTAACCTCTCCTTTGCCTTCCCCTCTCAACTCATGGTCCCTCACCAATCTTACCAACCTTTGCAGTTGGAATGCTGTTGTTTGTGACAACACCAATACAACTGTCTTAATCATAAACTTGTCTAATGCCAATCTCAGTGGAACACTCACTGACTTGGATTTTGCATCATTCCCAAACCTCACCCTCCTCAACCTCAATAGCAACAGGTTTGGTGGATCAATGCCATCATCAATTGGCAAACTCTCCAAGCTCAGTTTCTTAGACTTGGGAAACAATTTATTTGAAGGCACGCTACCGTCTGAGCTAGGCCAGCTAAGGGAACTTCAATATGTTAGTTTTTACAACAATAATCTCAATGGTACCATTCCCTATCAGCTCACTAATCTCCCCAAGGTAAGTTACCTTGAACTTGGATCAAACTTTTTTGTATCTTCCCTTGATTGGTCTCAATATTCAAACATGCTTTCCTTAAATTACCTTGGTTTGGAGGAAAATGAATTTACTGGTGACTTTCCAAGTTTCATACTTGAGTGCAAGAATTTAACATATCTTGACCTCTCTCAAAACACTTGGAATGGTACAATACCAGAATCCTTTTATGGTAATTTAGGAAAGCTTGAATATCTCAACCTCACCAACTGTGGACTAGAAGGAAAACTATCATCCAACTTGTCCCTTCTCTCGAATCTCAAAGATCTTCGCATTGGTAATAACATGTTTAATAGTCATATTCCAACAGAGATAGGATTGATATCTAAGCTTCAATTTCTTGAGCTGAACAACATTTCAGCTCATGGGGAAATTCCTTCTTCCATAGGTCAACTAAAGGAGTTAGTGCATCTTGATCTCAGTGCTAACTTTTTGAACTCTAAAGTTCCTTCTGAGCTTGGCCTATGCACAAATCTAACTTTCCTAAGCTTGGCAGTAAATAACCTGACAGGTTCTTTGCCTTTGTCATTAGCAAAGTTGACTAAATTATCAGAACTAGGATTATCAGATAATTCTTTTTCTGGTCAAATTTCAGCTTCATTGATCTCTAATTGGACTGAGTTAACTTCTTTGCAAGTCCAAGAAAATAGGTTAACAGGAATGGTACCTCCACAAATTGGTTTGTTGAAAAAAATTAAAATCCTTTTTCTCTTCAAAAATCTTTTCTCCGGTCCCATTCCTGAAGAGTTAGGAAACTTAAAGGAAATGACAGAGTTAGACCTTTCTGGAAACCATTTCTCTGGTCCAATTCCACCAACCATTTGGAATCTCACAAACATAACAGTCATAAATCTTTTTTTCAACAACCTCTCTGGAAACATTCCACTGGATATTGGAAACTTAACCTCACTACAAATTTTTGATGTCAACAATAATAACTTGGATGGAGAATTGCCACAAACAATTTCTCATCTAACTTCCTTAACCTATTTTTCTGTGTTCACCAATAACTTCTCAGGTAACATTTCCAGAGACTTTGGAAAGAATAGTCCTTCTTTGACTCGTGTCTACTTTTCAAACAATAGTTTCTCAGGAGAACTTCCTTCTGAATTATGCAGTGGCCACAATCTAGTTGTTTTGGCAGTAAATAACAACAGCTTTTCAGGGCCATTGCCGAAATCTTTGAGAAATTGTTCATCATTTATAAGAATTAGGCTTGATGATAACCAGTTCAATGGAAATATCACAGAAGCATTTGGGATTTATCCAAATCTTAGTTTCATCTCACTTAGCAGAAATCGTCTCGTTGGTTATCTCTCTCCAGATTGGGGTAAATGTATCAGCTTAACTGAGATGGAAATGAGTGGAAACAAACTTTCTGGAAAAATTCCAACCGAGCTAAATAAGTTGAGTAAATTACAATTTCTTAGCCTGCACTCCAACGAATTCACCGGAAATATCCCACATGAAATTGGAAATATAAGTCTACTCTTCATGTTGAACTTGAGCAGGAACCATTTATCAGGAGAAATCCCAAACAGTATTGGAAGATTAGCTCAACTTAATATTGTTGATTTATCAGATAATAACTTCAGTGGAAGCATTCCTAAAGAGCTCGGTAATTGTAATCGGTTACTAAGCATGAACTTAAGTCATAATGATCTATCTGGCATGATGCCGTATGAACTAGGCAATTTGTATTCACTCCAATCCTTGTTAGATCTTAGCAGCAACAACCTTTCGGGAGAAATTCCTCAAAATCTTCAAAAGTTAGTATCATTGGAGATCCTCAATGTCTCACACAACAATCTATCTGGAACAATCCCGCAATCCTTTTCAAGTATGCCTAGCCTTCAATCAGTGGATTTTTCTTACAATAACTTGTCAGGTTCGATCCCAACAGGTGGTGTTTTTCAAACAGAAACTGCTGAAGCTTTTGTCGGAAACTCAGGTTTGTGTGGTGATGTAAAAGGATTAAGATGTGCCACAATATCGTCTCAAGAAGGTTCTGGAGAGGCCAACAAAAAGGTACTTCTTGGTGTTACTATATCAGTTGGTGGAGTACTATTTATAGGGATGATTTGTGCTGGAATCTTATTCTTTCAAAGGCAAGCCAAAAAACACGTTGAAAAATCCAAAAACATTGAAGACAATGATCAGAGTACTTTGTGCATGGTATGGGGAAGAGATGGAAAATTCACATTTTCTGATCTTGTTAAAGCAACCAATGATTTCAACGAAAAGTACTGCATTGGAAAAGGAGGATTTGGAAGTGTTTATAGAGCAGAACTACTTACAGGTCAGGTAGTTGCTATCAAAAGACTCAATATATCAGACTCTGATGATATTCCAGAAGTGAACCGCATGAGCTTCATGAATGAGATAAGCACGCTAACAGAAGTGAGGCACCGGAATATAATAAAGCTTTATGGTTTCTGTTCTATGAGGAGACAAATGTTCCTTGTTTATGAACATGTAGAGAAAGGAAGCTTGGGAAAAGTTTTGTATGCAGAAGGAAAATTGGAGCTAAGTTGGAGTACAAGGGTGGAAATTGTGCAAGGAATAGCACATTCAATTGCTTACCTGCATAGTGATTGCTCTCCACCAATTGTCCACAGAGATATAACATTGAATAACATACTACTTGATTCAGATTATGTACCTCATCTTGCAGATTTTGGCACTGCAAAGCTGCTAAGCTCTAACAATTCAACTTGGACCTCAGTTGCTGGATCCTATGGCTACATGGCTCCAGAGCTAGCGCAAACTATGAGGGTGACTGAAAAGTGTGATGTGTATAGTTTTGGAGTGGTGGTGTTAGAGATAATGATGGGAAAACACCCTGGAGAATTCTTGGGCACCCTAAATTCAAACAAGTCTTTAGCATCAATGGAAGTGCTTGTGAAGGATGTCGTGGACCAAAGGCTTCCACCTCCAACAGGGAAACTAGCTGAAACAATAGTGTTCGCCATGAATGTAGCTTTAGCATGCACACGCGCTGCTCCTGAGTCTAGACCCATGATGCGTTCTGTTGCACAAGAATTATCTGCTACCACACCGGCTCGTCTCTCTCAGCCATTCAGTATGATAACTGTAAGCAAGCTTACAGGATTCCATAAATAG |
| Protein sequence |
>Msa1077100 MATIQKVNALVFYILLISLLPLNITASLKTEAEALVKWKNSLTSPLPSPLNSWSLTNLTNLCSWNAVVCDNTNTTVLIINLSNANLSGTLTDLDFASFPNLTLLNLNSNRFGGSMPSSIGKLSKLSFLDLGNNLFEGTLPSELGQLRELQYVSFYNNNLNGTIPYQLTNLPKVSYLELGSNFFVSSLDWSQYSNMLSLNYLGLEENEFTGDFPSFILECKNLTYLDLSQNTWNGTIPESFYGNLGKLEYLNLTNCGLEGKLSSNLSLLSNLKDLRIGNNMFNSHIPTEIGLISKLQFLELNNISAHGEIPSSIGQLKELVHLDLSANFLNSKVPSELGLCTNLTFLSLAVNNLTGSLPLSLAKLTKLSELGLSDNSFSGQISASLISNWTELTSLQVQENRLTGMVPPQIGLLKKIKILFLFKNLFSGPIPEELGNLKEMTELDLSGNHFSGPIPPTIWNLTNITVINLFFNNLSGNIPLDIGNLTSLQIFDVNNNNLDGELPQTISHLTSLTYFSVFTNNFSGNISRDFGKNSPSLTRVYFSNNSFSGELPSELCSGHNLVVLAVNNNSFSGPLPKSLRNCSSFIRIRLDDNQFNGNITEAFGIYPNLSFISLSRNRLVGYLSPDWGKCISLTEMEMSGNKLSGKIPTELNKLSKLQFLSLHSNEFTGNIPHEIGNISLLFMLNLSRNHLSGEIPNSIGRLAQLNIVDLSDNNFSGSIPKELGNCNRLLSMNLSHNDLSGMMPYELGNLYSLQSLLDLSSNNLSGEIPQNLQKLVSLEILNVSHNNLSGTIPQSFSSMPSLQSVDFSYNNLSGSIPTGGVFQTETAEAFVGNSGLCGDVKGLRCATISSQEGSGEANKKVLLGVTISVGGVLFIGMICAGILFFQRQAKKHVEKSKNIEDNDQSTLCMVWGRDGKFTFSDLVKATNDFNEKYCIGKGGFGSVYRAELLTGQVVAIKRLNISDSDDIPEVNRMSFMNEISTLTEVRHRNIIKLYGFCSMRRQMFLVYEHVEKGSLGKVLYAEGKLELSWSTRVEIVQGIAHSIAYLHSDCSPPIVHRDITLNNILLDSDYVPHLADFGTAKLLSSNNSTWTSVAGSYGYMAPELAQTMRVTEKCDVYSFGVVVLEIMMGKHPGEFLGTLNSNKSLASMEVLVKDVVDQRLPPPTGKLAETIVFAMNVALACTRAAPESRPMMRSVAQELSATTPARLSQPFSMITVSKLTGFHK* |
