AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1164980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1164980 MtrunA17_Chr7g0273111 94.521 73 4 0 1 73 1 73 2.54e-47 145
Msa1164980 MtrunA17_Chr1g0191461 67.742 62 20 0 11 72 7 68 5.95e-24 92.4
Msa1164980 MtrunA17_Chr1g0191481 48.077 52 27 0 8 59 3 54 5.40e-13 58.2
Msa1164980 MtrunA17_Chr7g0273101 58.824 51 19 2 23 72 17 66 3.73e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1164980 AT1G54790.2 52.632 57 27 0 16 72 12 68 1.13e-15 70.1
Msa1164980 AT1G54790.1 52.632 57 27 0 16 72 12 68 1.17e-15 70.1
Msa1164980 AT3G26430.1 48.438 64 30 2 11 72 5 67 1.59e-11 58.5
Msa1164980 AT3G05180.1 54.000 50 17 1 32 75 33 82 7.60e-11 56.6

Find 110 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTTCTCCTTTGGGATTC+AGG 0.044235 7_4:-56824923 Msa1164980:three_prime_UTR
GCCATTCTTAAATGCATATT+TGG 0.136590 7_4:-56825034 Msa1164980:three_prime_UTR
TTACAACCTTTCCTAAAATT+TGG 0.148502 7_4:+56824997 None:intergenic
CCCTCTGCCCTTTCTCCTTT+GGG 0.151136 7_4:-56824930 Msa1164980:three_prime_UTR
AATAAGCACATTATTATTCT+TGG 0.193684 7_4:+56826629 None:intergenic
TGATGGATCAGTTCCAAATT+TGG 0.197023 7_4:+56824403 None:intergenic
GCTCAGAATGTAGCCAAATT+TGG 0.208634 7_4:-56824416 Msa1164980:three_prime_UTR
TCCCTCTGCCCTTTCTCCTT+TGG 0.229352 7_4:-56824931 Msa1164980:three_prime_UTR
AGAATTTGAATCTGGAATTA+AGG 0.237994 7_4:-56824614 Msa1164980:intron
TTTCAGCTTGTTCCACCTTA+TGG 0.261204 7_4:-56826480 Msa1164980:CDS
AGCATGAAGTTGTAGGTTAA+AGG 0.261879 7_4:+56824331 None:intergenic
CCTAGTTCATCAAGCTTTGA+TGG 0.273723 7_4:+56824386 None:intergenic
CGCGCTCTTGAATTGCTTGC+AGG 0.276885 7_4:-56824874 Msa1164980:three_prime_UTR
TACGTATCCGCCACTTAATT+TGG 0.306008 7_4:-56826713 Msa1164980:five_prime_UTR
AAATGCATATTTGGATTCAT+TGG 0.318406 7_4:-56825025 Msa1164980:three_prime_UTR
TAGTATAGTATAAATATAAA+AGG 0.319886 7_4:-56826771 Msa1164980:five_prime_UTR
TGCTCTGTGTAGTAAATTGC+AGG 0.331048 7_4:-56824312 Msa1164980:three_prime_UTR
TATCCACAACATGCCATAAT+AGG 0.340753 7_4:+56823906 None:intergenic
TCAAATTCTAAAAGAATTGT+TGG 0.351758 7_4:+56824628 None:intergenic
ATTTGTACATTAATACATTC+AGG 0.356143 7_4:-56823482 Msa1164980:three_prime_UTR
TGTAATTGTCGTTCCATTGA+AGG 0.359086 7_4:+56823830 None:intergenic
CAACTTAGGTGATTCAAATT+CGG 0.362793 7_4:-56826532 Msa1164980:CDS
ATTATGGCATGTTGTGGATA+TGG 0.374728 7_4:-56823903 Msa1164980:three_prime_UTR
CACTTAATTTGGTTGATAGT+TGG 0.375833 7_4:-56826702 Msa1164980:five_prime_UTR
AATACATTCAGGAGGCTTCA+TGG 0.378589 7_4:-56823471 Msa1164980:three_prime_UTR
CACCGCTCTGTATTCCTATC+AGG 0.382976 7_4:+56823373 None:intergenic
AGGGTTTGAACAACCTATTA+TGG 0.383539 7_4:-56823919 Msa1164980:three_prime_UTR
CTTTATGTGCTTGTATTGTA+TGG 0.384323 7_4:-56823611 Msa1164980:three_prime_UTR
GCCATCACAGCCCTTCCATT+TGG 0.390999 7_4:+56826438 None:intergenic
AATGCATATTTGGATTCATT+GGG 0.397716 7_4:-56825024 Msa1164980:three_prime_UTR
ATGCATGGAAAATGCCTGAT+AGG 0.405670 7_4:-56823387 Msa1164980:three_prime_UTR
ATGCACCAGCAAGATCATTC+TGG 0.412020 7_4:+56824688 None:intergenic
CTTGAATTGCTTGCAGGAAA+AGG 0.426571 7_4:-56824868 Msa1164980:intron
TTGAAGTAGTTCTGTCCATA+AGG 0.431121 7_4:+56826465 None:intergenic
TTTGAAAGTATGAGATGAGT+TGG 0.433100 7_4:+56826674 None:intergenic
ACATATGTGACATTTGAATC+TGG 0.436387 7_4:+56824281 None:intergenic
GGTTGTAACTTTGCAGCAGC+TGG 0.442366 7_4:-56824982 Msa1164980:three_prime_UTR
TCACCTAAGTTGAAAACTGC+TGG 0.446971 7_4:+56826543 None:intergenic
AACTACTTCAAAACTCCAAA+TGG 0.448519 7_4:-56826453 Msa1164980:CDS
CCATCAAAGCTTGATGAACT+AGG 0.448934 7_4:-56824386 Msa1164980:three_prime_UTR
GATACACAATACAGGTCCTC+TGG 0.451058 7_4:-56824447 Msa1164980:three_prime_UTR
TACAGGTCCTCTGGGATGCT+TGG 0.454524 7_4:-56824438 Msa1164980:three_prime_UTR
TCCAAATATGCATTTAAGAA+TGG 0.454805 7_4:+56825033 None:intergenic
ACAGTTCTGAGTATATCAAC+TGG 0.456549 7_4:-56823791 Msa1164980:three_prime_UTR
TACACAGAGCATGAAGTTGT+AGG 0.457023 7_4:+56824324 None:intergenic
CATCTCATACTTTCAAACTA+AGG 0.459964 7_4:-56826668 Msa1164980:five_prime_UTR
GGGAACACAAAGACCTTCAA+TGG 0.460647 7_4:-56823843 Msa1164980:three_prime_UTR
GGTGAACTTACAGTTGGGTT+GGG 0.464328 7_4:-56826504 Msa1164980:CDS
ATTGCAAATTATTCTAGATA+CGG 0.471729 7_4:-56824230 Msa1164980:intron
GGAACGACAATTACAGCAAA+AGG 0.471811 7_4:-56823822 Msa1164980:three_prime_UTR
CAGAGGGATGATGCAGTGGC+TGG 0.475339 7_4:+56824946 None:intergenic
ACCTGAATCCCAAAGGAGAA+AGG 0.487971 7_4:+56824922 None:intergenic
TTGGAATGGAGGCAAGTACT+TGG 0.488366 7_4:+56824647 None:intergenic
ATTGGCCAGAATGATCTTGC+TGG 0.494393 7_4:-56824693 Msa1164980:three_prime_UTR
GTTGTTTGCAGTGGATGCAT+TGG 0.498025 7_4:-56825061 Msa1164980:intron
TGGTTCACATGTCTATGATA+AGG 0.501007 7_4:-56823451 Msa1164980:three_prime_UTR
TTTCCTTCAAAATCTATGCA+TGG 0.506879 7_4:-56823402 Msa1164980:three_prime_UTR
GACACAGGTGAACTTACAGT+TGG 0.510158 7_4:-56826510 Msa1164980:CDS
GAGCGCGAGCTTTGAACTTG+AGG 0.512637 7_4:+56824890 None:intergenic
TCCAAATGGAAGGGCTGTGA+TGG 0.516616 7_4:-56826439 Msa1164980:CDS
AACTTGAGCTTGAGAAGGAA+TGG 0.518487 7_4:+56823675 None:intergenic
CCTGAATCCCAAAGGAGAAA+GGG 0.521210 7_4:+56824923 None:intergenic
CAACTATCAACCAAATTAAG+TGG 0.523169 7_4:+56826703 None:intergenic
AGGTGAACTTACAGTTGGGT+TGG 0.523219 7_4:-56826505 Msa1164980:CDS
GGATTATACATGTTTGACAT+TGG 0.523669 7_4:-56824711 Msa1164980:three_prime_UTR
GATGAACTAGGATGTGTTAG+TGG 0.524106 7_4:-56824374 Msa1164980:three_prime_UTR
TTCTAAAAGAATTGTTGGAA+TGG 0.529731 7_4:+56824633 None:intergenic
TAAAAGAATTGTTGGAATGG+AGG 0.531513 7_4:+56824636 None:intergenic
ATCTCATACTTTCAAACTAA+GGG 0.533073 7_4:-56826667 Msa1164980:five_prime_UTR
ACTCTACTGTCATAGTTCAA+TGG 0.536947 7_4:+56823873 None:intergenic
ATGACAGTAGAGTGACTTGT+GGG 0.545670 7_4:-56823863 Msa1164980:three_prime_UTR
TATTTCTGGATACACAATAC+AGG 0.545778 7_4:-56824455 Msa1164980:three_prime_UTR
GTTGCATCACAGATTCTCAC+TGG 0.548587 7_4:-56823732 Msa1164980:three_prime_UTR
ATCTAGAATAATTTGCAATG+AGG 0.555564 7_4:+56824234 None:intergenic
TTTAGAACTTGAGCTTGAGA+AGG 0.563689 7_4:+56823670 None:intergenic
ACACAGGTGAACTTACAGTT+GGG 0.567683 7_4:-56826509 Msa1164980:CDS
TCTGAGTATATCAACTGGGA+TGG 0.570331 7_4:-56823786 Msa1164980:three_prime_UTR
TTCAAACTAAGGGCCATGGC+GGG 0.575921 7_4:-56826657 Msa1164980:exon
TGATAGAGACTATATGATGA+GGG 0.576750 7_4:-56824485 Msa1164980:intron
GGGATGATGCAGTGGCTGGA+AGG 0.577213 7_4:+56824950 None:intergenic
ACTTCAAAACTCCAAATGGA+AGG 0.581393 7_4:-56826449 Msa1164980:CDS
AAGTAGTTCTGTCCATAAGG+TGG 0.589355 7_4:+56826468 None:intergenic
GGTGATTCAAATTCGGACAC+AGG 0.594936 7_4:-56826525 Msa1164980:CDS
TTGATAGAGACTATATGATG+AGG 0.597221 7_4:-56824486 Msa1164980:intron
ATTCTTGGAATCACCCGCCA+TGG 0.603699 7_4:+56826644 None:intergenic
ATATATGAATATGATGCTCA+AGG 0.606900 7_4:+56826793 None:intergenic
TACTTTCAAACTAAGGGCCA+TGG 0.609071 7_4:-56826661 Msa1164980:exon
CAACCTATTATGGCATGTTG+TGG 0.613380 7_4:-56823909 Msa1164980:three_prime_UTR
CAGTTCTGAGTATATCAACT+GGG 0.617548 7_4:-56823790 Msa1164980:three_prime_UTR
ATACACAATACAGGTCCTCT+GGG 0.618984 7_4:-56824446 Msa1164980:three_prime_UTR
CCCAAAGGAGAAAGGGCAGA+GGG 0.623206 7_4:+56824930 None:intergenic
AGGGCAGAGGGATGATGCAG+TGG 0.626862 7_4:+56824942 None:intergenic
TCCCAAAGGAGAAAGGGCAG+AGG 0.629994 7_4:+56824929 None:intergenic
ATAGAGACTATATGATGAGG+GGG 0.637313 7_4:-56824483 Msa1164980:intron
TGTACATTAATACATTCAGG+AGG 0.638548 7_4:-56823479 Msa1164980:three_prime_UTR
CTTCAAAACTCCAAATGGAA+GGG 0.639200 7_4:-56826448 Msa1164980:CDS
TTTCAAACTAAGGGCCATGG+CGG 0.642095 7_4:-56826658 Msa1164980:exon
CTACTGTCATAGTTCAATGG+TGG 0.651617 7_4:+56823876 None:intergenic
GATAGAGACTATATGATGAG+GGG 0.655430 7_4:-56824484 Msa1164980:intron
CTATCAACCAAATTAAGTGG+CGG 0.658914 7_4:+56826706 None:intergenic
TATGACAGTAGAGTGACTTG+TGG 0.659401 7_4:-56823864 Msa1164980:three_prime_UTR
GCTCTGTGTAGTAAATTGCA+GGG 0.661282 7_4:-56824311 Msa1164980:three_prime_UTR
CTGAGACACCTGAATCCCAA+AGG 0.666075 7_4:+56824915 None:intergenic
ATGAACTAGGATGTGTTAGT+GGG 0.666553 7_4:-56824373 Msa1164980:three_prime_UTR
TTAGGAAGTCAACAATGAGA+CGG 0.673079 7_4:+56826416 None:intergenic
ATGGCATGTTGTGGATATGG+AGG 0.676401 7_4:-56823900 Msa1164980:three_prime_UTR
TCACTAATGTCAAATTGACA+TGG 0.677077 7_4:-56823534 Msa1164980:three_prime_UTR
AATTCTTTAACATCATCACA+CGG 0.695860 7_4:+56823502 None:intergenic
TTCTGAGCCAAGCATCCCAG+AGG 0.701144 7_4:+56824431 None:intergenic
TGCCTGATAGGAATACAGAG+CGG 0.807319 7_4:-56823375 Msa1164980:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTATAGTATAAATATAAA+AGG - chr7_4:56823397-56823416 Msa1164980:three_prime_UTR 10.0%
!!! ACTTTGTTTTTTTAAAATAA+AGG - chr7_4:56823906-56823925 Msa1164980:three_prime_UTR 10.0%
!!! AATTAATGTATTTTTTTTAC+AGG - chr7_4:56824231-56824250 Msa1164980:three_prime_UTR 10.0%
!!! ATCTTATTTAAATATGTTTT+TGG + chr7_4:56824316-56824335 None:intergenic 10.0%
!! TGATTTATGATATAATATTA+TGG - chr7_4:56824355-56824374 Msa1164980:three_prime_UTR 10.0%
!! TAAATATCATAACATAAATA+AGG - chr7_4:56824455-56824474 Msa1164980:three_prime_UTR 10.0%
!!! AAAAAACATATTTCTTTTAC+TGG + chr7_4:56823947-56823966 None:intergenic 15.0%
!! TACAATGTAAAATATATTTC+AGG + chr7_4:56824020-56824039 None:intergenic 15.0%
!! ATATTACACACATTATAAAT+TGG - chr7_4:56824099-56824118 Msa1164980:intron 15.0%
!! TAAAAGTAAAATGTATTTGT+AGG - chr7_4:56824289-56824308 Msa1164980:three_prime_UTR 15.0%
!! TTTAGGTAAGTATAATAATT+TGG - chr7_4:56824492-56824511 Msa1164980:intron 15.0%
!! ATTGTTAGATAGAAAAATAA+TGG - chr7_4:56824673-56824692 Msa1164980:three_prime_UTR 15.0%
!!! AATGCAAACTTTTTAATTTA+CGG + chr7_4:56825048-56825067 None:intergenic 15.0%
!! AAAATTCAAAACTAAATTAG+TGG + chr7_4:56825335-56825354 None:intergenic 15.0%
!!! TATTACATTTATCTTTTTCA+CGG - chr7_4:56825975-56825994 Msa1164980:intron 15.0%
!! TGTAATATTTATATATTTGC+AGG - chr7_4:56826229-56826248 Msa1164980:intron 15.0%
!! GTAATATTTATATATTTGCA+GGG - chr7_4:56826230-56826249 Msa1164980:intron 15.0%
!! AATAAGCACATTATTATTCT+TGG + chr7_4:56823542-56823561 None:intergenic 20.0%
!! AAAGAAAAAAAAAAGTTGCA+AGG + chr7_4:56823972-56823991 None:intergenic 20.0%
!!! GCAACTTTTTTTTTTCTTTT+TGG - chr7_4:56823973-56823992 Msa1164980:intron 20.0%
!!! TTCATTTTAGTCATTATGTT+TGG + chr7_4:56824080-56824099 None:intergenic 20.0%
!! AAGTATAATAATTTGGACAA+AGG - chr7_4:56824499-56824518 Msa1164980:intron 20.0%
!! AATAATCAATTAATTAGCCA+TGG - chr7_4:56824779-56824798 Msa1164980:intron 20.0%
!! TATGCAAATTATACATATGA+TGG + chr7_4:56824808-56824827 None:intergenic 20.0%
!!! TCAAATTCTAAAAGAATTGT+TGG + chr7_4:56825543-56825562 None:intergenic 20.0%
!!! ATTCTTTTAGAATTTGAATC+TGG - chr7_4:56825546-56825565 Msa1164980:intron 20.0%
!!! GCTTTCATTTTGTTTATTTT+TGG - chr7_4:56825594-56825613 Msa1164980:intron 20.0%
!!! TGAGAATTCTTTTATGTTAA+TGG + chr7_4:56825646-56825665 None:intergenic 20.0%
!! ATTGCAAATTATTCTAGATA+CGG - chr7_4:56825938-56825957 Msa1164980:intron 20.0%
!!! ATTACATTTATCTTTTTCAC+GGG - chr7_4:56825976-56825995 Msa1164980:intron 20.0%
!! AATCATAATACAAACTCTAA+AGG + chr7_4:56826007-56826026 None:intergenic 20.0%
!!! ATTTTATACATATACATCGT+CGG - chr7_4:56826024-56826043 Msa1164980:intron 20.0%
!! ATTACAAATTTAACAACTCT+TGG + chr7_4:56826215-56826234 None:intergenic 20.0%
!! ATTTGTACATTAATACATTC+AGG - chr7_4:56826686-56826705 Msa1164980:five_prime_UTR 20.0%
! ATATATGAATATGATGCTCA+AGG + chr7_4:56823378-56823397 None:intergenic 25.0%
! TACCATCAAAAAAAGTAAGT+TGG - chr7_4:56823432-56823451 Msa1164980:three_prime_UTR 25.0%
!!! TACCAACTTACTTTTTTTGA+TGG + chr7_4:56823437-56823456 None:intergenic 25.0%
! ATCTCATACTTTCAAACTAA+GGG - chr7_4:56823501-56823520 Msa1164980:three_prime_UTR 25.0%
!! TTTTAGTCATTATGTTTGGA+AGG + chr7_4:56824076-56824095 None:intergenic 25.0%
!! CAACATGAACAAATTTTACA+AGG - chr7_4:56824129-56824148 Msa1164980:intron 25.0%
! ACCAATACTTTCAATTTGAT+TGG - chr7_4:56824169-56824188 Msa1164980:intron 25.0%
! CCAATACTTTCAATTTGATT+GGG - chr7_4:56824170-56824189 Msa1164980:intron 25.0%
!!! CAAGTTTTATTCCATTAAGT+AGG + chr7_4:56824405-56824424 None:intergenic 25.0%
! CTACTTAATGGAATAAAACT+TGG - chr7_4:56824403-56824422 Msa1164980:three_prime_UTR 25.0%
! CATAAATAAGGACATATGAT+AGG - chr7_4:56824467-56824486 Msa1164980:three_prime_UTR 25.0%
! TTGATGTTTCTTTGTATGTT+TGG + chr7_4:56824654-56824673 None:intergenic 25.0%
!! AAAATCTAAATGGATGGATT+CGG + chr7_4:56824859-56824878 None:intergenic 25.0%
! TAAGAAAATCAATGATGAGA+CGG + chr7_4:56825010-56825029 None:intergenic 25.0%
! TCCAAATATGCATTTAAGAA+TGG + chr7_4:56825138-56825157 None:intergenic 25.0%
! AAATGCATATTTGGATTCAT+TGG - chr7_4:56825143-56825162 Msa1164980:intron 25.0%
! AATGCATATTTGGATTCATT+GGG - chr7_4:56825144-56825163 Msa1164980:intron 25.0%
! TTACAACCTTTCCTAAAATT+TGG + chr7_4:56825174-56825193 None:intergenic 25.0%
!! GTGCATTTTATTCAAAACTT+TGG - chr7_4:56825497-56825516 Msa1164980:intron 25.0%
!! TTCTAAAAGAATTGTTGGAA+TGG + chr7_4:56825538-56825557 None:intergenic 25.0%
!! AGAATTTGAATCTGGAATTA+AGG - chr7_4:56825554-56825573 Msa1164980:intron 25.0%
!! ATCTAGAATAATTTGCAATG+AGG + chr7_4:56825937-56825956 None:intergenic 25.0%
! AATTCTTTAACATCATCACA+CGG + chr7_4:56826669-56826688 None:intergenic 25.0%
CAACTATCAACCAAATTAAG+TGG + chr7_4:56823468-56823487 None:intergenic 30.0%
!! CACTTAATTTGGTTGATAGT+TGG - chr7_4:56823466-56823485 Msa1164980:three_prime_UTR 30.0%
! TTTGAAAGTATGAGATGAGT+TGG + chr7_4:56823497-56823516 None:intergenic 30.0%
CATCTCATACTTTCAAACTA+AGG - chr7_4:56823500-56823519 Msa1164980:three_prime_UTR 30.0%
!!! TGTGTTATTTTGCACATGTT+TGG - chr7_4:56823573-56823592 Msa1164980:three_prime_UTR 30.0%
CAACTTAGGTGATTCAAATT+CGG - chr7_4:56823636-56823655 Msa1164980:three_prime_UTR 30.0%
AACTACTTCAAAACTCCAAA+TGG - chr7_4:56823715-56823734 Msa1164980:three_prime_UTR 30.0%
ACAAGGACTAAAAAACATCA+AGG - chr7_4:56824146-56824165 Msa1164980:intron 30.0%
! CCCAATCAAATTGAAAGTAT+TGG + chr7_4:56824173-56824192 None:intergenic 30.0%
TAATTTGGACAAAGGAATCT+TGG - chr7_4:56824507-56824526 Msa1164980:intron 30.0%
TTCAACAACATTGCACTATT+TGG + chr7_4:56824584-56824603 None:intergenic 30.0%
TGTTTCTTTGTATGTTTGGT+GGG + chr7_4:56824650-56824669 None:intergenic 30.0%
ATGTTTCTTTGTATGTTTGG+TGG + chr7_4:56824651-56824670 None:intergenic 30.0%
GCAAATTATACATATGATGG+TGG + chr7_4:56824805-56824824 None:intergenic 30.0%
AAACTGCTGGAAAATCTAAA+TGG + chr7_4:56824869-56824888 None:intergenic 30.0%
! TTTTCCTAATGATGTTGTCT+TGG - chr7_4:56825082-56825101 Msa1164980:intron 30.0%
GCCATTCTTAAATGCATATT+TGG - chr7_4:56825134-56825153 Msa1164980:intron 30.0%
TTCTCAAAAATATCTTCGCT+CGG + chr7_4:56825436-56825455 None:intergenic 30.0%
!! AGCGAAGATATTTTTGAGAA+AGG - chr7_4:56825436-56825455 Msa1164980:intron 30.0%
GGATTATACATGTTTGACAT+TGG - chr7_4:56825457-56825476 Msa1164980:intron 30.0%
TAAAAGAATTGTTGGAATGG+AGG + chr7_4:56825535-56825554 None:intergenic 30.0%
! TTTGAATCTGGAATTAAGGT+TGG - chr7_4:56825558-56825577 Msa1164980:intron 30.0%
TTGATAGAGACTATATGATG+AGG - chr7_4:56825682-56825701 Msa1164980:intron 30.0%
TGATAGAGACTATATGATGA+GGG - chr7_4:56825683-56825702 Msa1164980:intron 30.0%
!! TATTTCTGGATACACAATAC+AGG - chr7_4:56825713-56825732 Msa1164980:intron 30.0%
ACATATGTGACATTTGAATC+TGG + chr7_4:56825890-56825909 None:intergenic 30.0%
CTTTATGTGCTTGTATTGTA+TGG - chr7_4:56826557-56826576 Msa1164980:CDS 30.0%
TCACTAATGTCAAATTGACA+TGG - chr7_4:56826634-56826653 Msa1164980:CDS 30.0%
TGTACATTAATACATTCAGG+AGG - chr7_4:56826689-56826708 Msa1164980:five_prime_UTR 30.0%
TTTCCTTCAAAATCTATGCA+TGG - chr7_4:56826766-56826785 Msa1164980:five_prime_UTR 30.0%
! TTTCCATGCATAGATTTTGA+AGG + chr7_4:56826772-56826791 None:intergenic 30.0%
CTATCAACCAAATTAAGTGG+CGG + chr7_4:56823465-56823484 None:intergenic 35.0%
! ATTTTGCACATGTTTGGAAG+TGG - chr7_4:56823579-56823598 Msa1164980:three_prime_UTR 35.0%
! TTTCCAGCAGTTTTCAACTT+AGG - chr7_4:56823622-56823641 Msa1164980:three_prime_UTR 35.0%
! TTGAAGTAGTTCTGTCCATA+AGG + chr7_4:56823706-56823725 None:intergenic 35.0%
ACTTCAAAACTCCAAATGGA+AGG - chr7_4:56823719-56823738 Msa1164980:three_prime_UTR 35.0%
CTTCAAAACTCCAAATGGAA+GGG - chr7_4:56823720-56823739 Msa1164980:three_prime_UTR 35.0%
TTAGGAAGTCAACAATGAGA+CGG + chr7_4:56823755-56823774 None:intergenic 35.0%
GGAACTAAAAGATACACAGA+GGG + chr7_4:56823999-56824018 None:intergenic 35.0%
AGGAACTAAAAGATACACAG+AGG + chr7_4:56824000-56824019 None:intergenic 35.0%
ATGTAGCTGATCCTACTTAA+TGG - chr7_4:56824391-56824410 Msa1164980:three_prime_UTR 35.0%
AGGACATATGATAGGAGTTT+AGG - chr7_4:56824475-56824494 Msa1164980:three_prime_UTR 35.0%
ACAACATTGCACTATTTGGA+TGG + chr7_4:56824580-56824599 None:intergenic 35.0%
TTTCAAAAACAAGCAAACCC+TGG - chr7_4:56824727-56824746 Msa1164980:three_prime_UTR 35.0%
!! TATGTGTTTTAAGCCTTCCA+GGG + chr7_4:56824747-56824766 None:intergenic 35.0%
GGCACAAGGTAATAAGAATA+TGG + chr7_4:56824838-56824857 None:intergenic 35.0%
TGCTGGAAAATCTAAATGGA+TGG + chr7_4:56824865-56824884 None:intergenic 35.0%
! TTTCCAGCAGTTTTCAACTT+TGG - chr7_4:56823622-56823641 Msa1164980:three_prime_UTR 35.0%
TGAAAGTAAGTGTGTCCATT+TGG + chr7_4:56824960-56824979 None:intergenic 35.0%
ACTTACTTTCATCTACCATC+AGG - chr7_4:56824969-56824988 Msa1164980:three_prime_UTR 35.0%
CTTACTTTCATCTACCATCA+GGG - chr7_4:56824970-56824989 Msa1164980:three_prime_UTR 35.0%
ACAACCAAGACAACATCATT+AGG + chr7_4:56825089-56825108 None:intergenic 35.0%
! CATTGGGTTTGCCAAATTTT+AGG - chr7_4:56825160-56825179 Msa1164980:intron 35.0%
!! GGTTTGCCAAATTTTAGGAA+AGG - chr7_4:56825165-56825184 Msa1164980:intron 35.0%
TTCACTCTTTCAAGCATTTC+AGG - chr7_4:56825388-56825407 Msa1164980:intron 35.0%
TCTCAAAAATATCTTCGCTC+GGG + chr7_4:56825435-56825454 None:intergenic 35.0%
GATAGAGACTATATGATGAG+GGG - chr7_4:56825684-56825703 Msa1164980:intron 35.0%
ATAGAGACTATATGATGAGG+GGG - chr7_4:56825685-56825704 Msa1164980:intron 35.0%
! TGATGGATCAGTTCCAAATT+TGG + chr7_4:56825768-56825787 None:intergenic 35.0%
ATGAACTAGGATGTGTTAGT+GGG - chr7_4:56825795-56825814 Msa1164980:intron 35.0%
AGCATGAAGTTGTAGGTTAA+AGG + chr7_4:56825840-56825859 None:intergenic 35.0%
TCTAGATACGGTTAGTAACT+TGG - chr7_4:56825950-56825969 Msa1164980:intron 35.0%
TCACAATCAATCATACGTGT+GGG + chr7_4:56826121-56826140 None:intergenic 35.0%
ATCACAATCAATCATACGTG+TGG + chr7_4:56826122-56826141 None:intergenic 35.0%
AGGGTTTGAACAACCTATTA+TGG - chr7_4:56826249-56826268 Msa1164980:intron 35.0%
TATCCACAACATGCCATAAT+AGG + chr7_4:56826265-56826284 None:intergenic 35.0%
ATTATGGCATGTTGTGGATA+TGG - chr7_4:56826265-56826284 Msa1164980:intron 35.0%
ACTCTACTGTCATAGTTCAA+TGG + chr7_4:56826298-56826317 None:intergenic 35.0%
TGTAATTGTCGTTCCATTGA+AGG + chr7_4:56826341-56826360 None:intergenic 35.0%
!! ACAGTTCTGAGTATATCAAC+TGG - chr7_4:56826377-56826396 Msa1164980:intron 35.0%
!! CAGTTCTGAGTATATCAACT+GGG - chr7_4:56826378-56826397 Msa1164980:intron 35.0%
! AATGGCATTTTGTCTGAGAA+AGG + chr7_4:56826478-56826497 None:intergenic 35.0%
! TTTAGAACTTGAGCTTGAGA+AGG + chr7_4:56826501-56826520 None:intergenic 35.0%
TGGTTCACATGTCTATGATA+AGG - chr7_4:56826717-56826736 Msa1164980:five_prime_UTR 35.0%
TACGTATCCGCCACTTAATT+TGG - chr7_4:56823455-56823474 Msa1164980:three_prime_UTR 40.0%
TACTTTCAAACTAAGGGCCA+TGG - chr7_4:56823507-56823526 Msa1164980:three_prime_UTR 40.0%
! TCACCTAAGTTGAAAACTGC+TGG + chr7_4:56823628-56823647 None:intergenic 40.0%
ACACAGGTGAACTTACAGTT+GGG - chr7_4:56823659-56823678 Msa1164980:three_prime_UTR 40.0%
! TTTCAGCTTGTTCCACCTTA+TGG - chr7_4:56823688-56823707 Msa1164980:three_prime_UTR 40.0%
! AAGTAGTTCTGTCCATAAGG+TGG + chr7_4:56823703-56823722 None:intergenic 40.0%
! ATCTTGGTTCTCTCTAGATC+AGG - chr7_4:56824523-56824542 Msa1164980:intron 40.0%
TAAAAGTGAGTCATGCGACA+AGG - chr7_4:56824549-56824568 Msa1164980:intron 40.0%
AAAAACAAGCAAACCCTGGA+AGG - chr7_4:56824731-56824750 Msa1164980:three_prime_UTR 40.0%
!! GTATGTGTTTTAAGCCTTCC+AGG + chr7_4:56824748-56824767 None:intergenic 40.0%
!! TATACATATGATGGTGGCCA+TGG + chr7_4:56824799-56824818 None:intergenic 40.0%
TCACCAAAGTTGAAAACTGC+TGG + chr7_4:56824882-56824901 None:intergenic 40.0%
TTCAAAACCAGCGGTAACAA+GGG + chr7_4:56824925-56824944 None:intergenic 40.0%
TTTCAAAACCAGCGGTAACA+AGG + chr7_4:56824926-56824945 None:intergenic 40.0%
GTAAAGGCTTTCAAAACCAG+CGG + chr7_4:56824934-56824953 None:intergenic 40.0%
AGTAAGTGTGTCCATTTGGT+GGG + chr7_4:56824956-56824975 None:intergenic 40.0%
AAGTAAGTGTGTCCATTTGG+TGG + chr7_4:56824957-56824976 None:intergenic 40.0%
CTTGAATTGCTTGCAGGAAA+AGG - chr7_4:56825300-56825319 Msa1164980:intron 40.0%
ATACACAATACAGGTCCTCT+GGG - chr7_4:56825722-56825741 Msa1164980:intron 40.0%
GCTCAGAATGTAGCCAAATT+TGG - chr7_4:56825752-56825771 Msa1164980:intron 40.0%
! CCTAGTTCATCAAGCTTTGA+TGG + chr7_4:56825785-56825804 None:intergenic 40.0%
CCATCAAAGCTTGATGAACT+AGG - chr7_4:56825782-56825801 Msa1164980:intron 40.0%
GATGAACTAGGATGTGTTAG+TGG - chr7_4:56825794-56825813 Msa1164980:intron 40.0%
!! GGTTAAAGGTTTTAGCAGCT+TGG + chr7_4:56825826-56825845 None:intergenic 40.0%
TACACAGAGCATGAAGTTGT+AGG + chr7_4:56825847-56825866 None:intergenic 40.0%
TGCTCTGTGTAGTAAATTGC+AGG - chr7_4:56825856-56825875 Msa1164980:intron 40.0%
GCTCTGTGTAGTAAATTGCA+GGG - chr7_4:56825857-56825876 Msa1164980:intron 40.0%
CAACCTATTATGGCATGTTG+TGG - chr7_4:56826259-56826278 Msa1164980:intron 40.0%
CTACTGTCATAGTTCAATGG+TGG + chr7_4:56826295-56826314 None:intergenic 40.0%
TATGACAGTAGAGTGACTTG+TGG - chr7_4:56826304-56826323 Msa1164980:intron 40.0%
! ATGACAGTAGAGTGACTTGT+GGG - chr7_4:56826305-56826324 Msa1164980:intron 40.0%
GGAACGACAATTACAGCAAA+AGG - chr7_4:56826346-56826365 Msa1164980:intron 40.0%
TCTGAGTATATCAACTGGGA+TGG - chr7_4:56826382-56826401 Msa1164980:intron 40.0%
! GCATTTTGTCTGAGAAAGGA+GGG + chr7_4:56826474-56826493 None:intergenic 40.0%
AACTTGAGCTTGAGAAGGAA+TGG + chr7_4:56826496-56826515 None:intergenic 40.0%
AATACATTCAGGAGGCTTCA+TGG - chr7_4:56826697-56826716 Msa1164980:five_prime_UTR 40.0%
ATGCATGGAAAATGCCTGAT+AGG - chr7_4:56826781-56826800 Msa1164980:five_prime_UTR 40.0%
TTTCAAACTAAGGGCCATGG+CGG - chr7_4:56823510-56823529 Msa1164980:three_prime_UTR 45.0%
GGTGATTCAAATTCGGACAC+AGG - chr7_4:56823643-56823662 Msa1164980:three_prime_UTR 45.0%
GACACAGGTGAACTTACAGT+TGG - chr7_4:56823658-56823677 Msa1164980:three_prime_UTR 45.0%
AGGTGAACTTACAGTTGGGT+TGG - chr7_4:56823663-56823682 Msa1164980:three_prime_UTR 45.0%
GGTGAACTTACAGTTGGGTT+GGG - chr7_4:56823664-56823683 Msa1164980:three_prime_UTR 45.0%
! AAATGGATGGATTCGGCACA+AGG + chr7_4:56824852-56824871 None:intergenic 45.0%
GGTGATTCAAATTCCGACAC+CGG - chr7_4:56824897-56824916 Msa1164980:three_prime_UTR 45.0%
GAAAGCCTTTACCCACCAAA+TGG - chr7_4:56824942-56824961 Msa1164980:three_prime_UTR 45.0%
! TGTGTCCATTTGGTGGGTAA+AGG + chr7_4:56824950-56824969 None:intergenic 45.0%
!! GTCTTGGTTGTTGTTTGCAG+TGG - chr7_4:56825098-56825117 Msa1164980:intron 45.0%
GTTGTTTGCAGTGGATGCAT+TGG - chr7_4:56825107-56825126 Msa1164980:intron 45.0%
CCTGAATCCCAAAGGAGAAA+GGG + chr7_4:56825248-56825267 None:intergenic 45.0%
ACCTGAATCCCAAAGGAGAA+AGG + chr7_4:56825249-56825268 None:intergenic 45.0%
ATTGGCCAGAATGATCTTGC+TGG - chr7_4:56825475-56825494 Msa1164980:intron 45.0%
ATGCACCAGCAAGATCATTC+TGG + chr7_4:56825483-56825502 None:intergenic 45.0%
TTGGAATGGAGGCAAGTACT+TGG + chr7_4:56825524-56825543 None:intergenic 45.0%
ATGAGGGGGCTAGATATTTC+TGG - chr7_4:56825699-56825718 Msa1164980:intron 45.0%
GATACACAATACAGGTCCTC+TGG - chr7_4:56825721-56825740 Msa1164980:intron 45.0%
ATGGCATGTTGTGGATATGG+AGG - chr7_4:56826268-56826287 Msa1164980:intron 45.0%
GGGAACACAAAGACCTTCAA+TGG - chr7_4:56826325-56826344 Msa1164980:intron 45.0%
GTTGCATCACAGATTCTCAC+TGG - chr7_4:56826436-56826455 Msa1164980:CDS 45.0%
! GGCATTTTGTCTGAGAAAGG+AGG + chr7_4:56826475-56826494 None:intergenic 45.0%
TGCCTGATAGGAATACAGAG+CGG - chr7_4:56826793-56826812 Msa1164980:five_prime_UTR 45.0%
!! ATAAAATAAAAATTATACTT+AGG + chr7_4:56823773-56823792 None:intergenic 5.0%
!! TAATATTAATAATCTATAAA+AGG + chr7_4:56823848-56823867 None:intergenic 5.0%
!! GTTAATAATTATAAATATAA+AGG - chr7_4:56823875-56823894 Msa1164980:three_prime_UTR 5.0%
!!! CTTTTATTTTTTATTTTTTA+AGG + chr7_4:56824275-56824294 None:intergenic 5.0%
TTCAAACTAAGGGCCATGGC+GGG - chr7_4:56823511-56823530 Msa1164980:three_prime_UTR 50.0%
ATTCTTGGAATCACCCGCCA+TGG + chr7_4:56823527-56823546 None:intergenic 50.0%
TCCAAATGGAAGGGCTGTGA+TGG - chr7_4:56823729-56823748 Msa1164980:three_prime_UTR 50.0%
! CCATCAGAGTATCTCCCTGA+TGG + chr7_4:56824987-56825006 None:intergenic 50.0%
CCATCAGGGAGATACTCTGA+TGG - chr7_4:56824984-56825003 Msa1164980:three_prime_UTR 50.0%
GGTTGTAACTTTGCAGCAGC+TGG - chr7_4:56825186-56825205 Msa1164980:intron 50.0%
!! CCCTTTCTCCTTTGGGATTC+AGG - chr7_4:56825245-56825264 Msa1164980:intron 50.0%
CTGAGACACCTGAATCCCAA+AGG + chr7_4:56825256-56825275 None:intergenic 50.0%
CACCGCTCTGTATTCCTATC+AGG + chr7_4:56826798-56826817 None:intergenic 50.0%
GCCATCACAGCCCTTCCATT+TGG + chr7_4:56823733-56823752 None:intergenic 55.0%
TCCCTCTGCCCTTTCTCCTT+TGG - chr7_4:56825237-56825256 Msa1164980:intron 55.0%
CCCAAAGGAGAAAGGGCAGA+GGG + chr7_4:56825241-56825260 None:intergenic 55.0%
CCCTCTGCCCTTTCTCCTTT+GGG - chr7_4:56825238-56825257 Msa1164980:intron 55.0%
TCCCAAAGGAGAAAGGGCAG+AGG + chr7_4:56825242-56825261 None:intergenic 55.0%
!! GAGCGCGAGCTTTGAACTTG+AGG + chr7_4:56825281-56825300 None:intergenic 55.0%
CGCGCTCTTGAATTGCTTGC+AGG - chr7_4:56825294-56825313 Msa1164980:intron 55.0%
!! TACAGGTCCTCTGGGATGCT+TGG - chr7_4:56825730-56825749 Msa1164980:intron 55.0%
TTCTGAGCCAAGCATCCCAG+AGG + chr7_4:56825740-56825759 None:intergenic 55.0%
!! GGTAACAAGGGTGCCGGTGT+CGG + chr7_4:56824913-56824932 None:intergenic 60.0%
! ACCAGCGGTAACAAGGGTGC+CGG + chr7_4:56824919-56824938 None:intergenic 60.0%
GGGATGATGCAGTGGCTGGA+AGG + chr7_4:56825221-56825240 None:intergenic 60.0%
CAGAGGGATGATGCAGTGGC+TGG + chr7_4:56825225-56825244 None:intergenic 60.0%
AGGGCAGAGGGATGATGCAG+TGG + chr7_4:56825229-56825248 None:intergenic 60.0%
ACCGGCACCCTTGTTACCGC+TGG - chr7_4:56824915-56824934 Msa1164980:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 56823355 56826835 56823355 ID=Msa1164980;Name=Msa1164980
chr7_4 mRNA 56823355 56826835 56823355 ID=Msa1164980-mRNA-1;Parent=Msa1164980;Name=Msa1164980-mRNA-1;_AED=0.30;_eAED=0.30;_QI=171|1|1|1|0.5|0.6|5|1226|82
chr7_4 exon 56823355 56823939 56823355 ID=Msa1164980-mRNA-1:exon:17046;Parent=Msa1164980-mRNA-1
chr7_4 exon 56824231 56824501 56824231 ID=Msa1164980-mRNA-1:exon:17045;Parent=Msa1164980-mRNA-1
chr7_4 exon 56824615 56824780 56824615 ID=Msa1164980-mRNA-1:exon:17044;Parent=Msa1164980-mRNA-1
chr7_4 exon 56824869 56825072 56824869 ID=Msa1164980-mRNA-1:exon:17043;Parent=Msa1164980-mRNA-1
chr7_4 exon 56826416 56826835 56826416 ID=Msa1164980-mRNA-1:exon:17042;Parent=Msa1164980-mRNA-1
chr7_4 five_prime_UTR 56826665 56826835 56826665 ID=Msa1164980-mRNA-1:five_prime_utr;Parent=Msa1164980-mRNA-1
chr7_4 CDS 56826416 56826664 56826416 ID=Msa1164980-mRNA-1:cds;Parent=Msa1164980-mRNA-1
chr7_4 three_prime_UTR 56824869 56825072 56824869 ID=Msa1164980-mRNA-1:three_prime_utr;Parent=Msa1164980-mRNA-1
chr7_4 three_prime_UTR 56824615 56824780 56824615 ID=Msa1164980-mRNA-1:three_prime_utr;Parent=Msa1164980-mRNA-1
chr7_4 three_prime_UTR 56824231 56824501 56824231 ID=Msa1164980-mRNA-1:three_prime_utr;Parent=Msa1164980-mRNA-1
chr7_4 three_prime_UTR 56823355 56823939 56823355 ID=Msa1164980-mRNA-1:three_prime_utr;Parent=Msa1164980-mRNA-1
Gene Sequence

>Msa1164980

ATGGCGGGTGATTCCAAGAATAATAATGTGCTTATTATTATTCATGTTGTGTTATTTTGCACATGTTTGGAAGTGGCAAATTCAGTTGAGTTCAATTTTCCAGCAGTTTTCAACTTAGGTGATTCAAATTCGGACACAGGTGAACTTACAGTTGGGTTGGGCTTTCAGCTTGTTCCACCTTATGGACAGAACTACTTCAAAACTCCAAATGGAAGGGCTGTGATGGCCGTCTCATTGTTGACTTCCTAA
Protein sequence

>Msa1164980

MAGDSKNNNVLIIIHVVLFCTCLEVANSVEFNFPAVFNLGDSNSDTGELTVGLGFQLVPPYGQNYFKTPNGRAVMAVSLLTS*