AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1176810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 105 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCACATGAACCAATTT+TGG 0.144068 8_1:-9024759 None:intergenic
CTCATAGCTTCTGCCTCTTT+TGG 0.173367 8_1:-9022720 None:intergenic
AACCATTAAGCAACCAAAAT+TGG 0.193707 8_1:+9024746 Msa1176810:three_prime_UTR
AGCGGTCCTTTAGATTATTT+TGG 0.206673 8_1:+9024304 Msa1176810:three_prime_UTR
TCAATTTATTATTATTATTC+AGG 0.218733 8_1:+9021212 Msa1176810:CDS
ACACTTTCCTGATATTATTA+TGG 0.221862 8_1:+9025136 Msa1176810:three_prime_UTR
AGTCTGTATGGCATTGGTTT+TGG 0.247931 8_1:+9024506 Msa1176810:three_prime_UTR
CAACAAAATATATATGGAAT+TGG 0.272940 8_1:+9022627 Msa1176810:CDS
GACCTTCTCAATTTATTATT+CGG 0.285539 8_1:+9022742 Msa1176810:CDS
TTCCTTGCCATAATAATATC+AGG 0.293804 8_1:-9025143 None:intergenic
AATTGTCTGTGCAATCTAAT+TGG 0.303919 8_1:-9020983 None:intergenic
AAGGAGTTGTTTCTTGTGTT+TGG 0.306092 8_1:-9022694 None:intergenic
CCTACGCCTACCAACATTCT+TGG 0.316532 8_1:-9024216 None:intergenic
GTTGTTTGTTTGTTTATAAT+AGG 0.322926 8_1:+9025028 Msa1176810:three_prime_UTR
AATGAGTTGTTTCTTGTGTT+TGG 0.324122 8_1:-9021157 None:intergenic
GGAGGTAGAACCAGAGGATT+TGG 0.334649 8_1:-9021108 None:intergenic
ATGGATGGGAGGATCGATTC+TGG 0.341412 8_1:+9021411 Msa1176810:CDS
ATGGATGGGAGGATCGATTC+TGG 0.341412 8_1:+9022901 Msa1176810:three_prime_UTR
GCTGTTGTTGGTATGTTTGC+AGG 0.349944 8_1:+9024152 Msa1176810:three_prime_UTR
TAGAGCTAAAATTCATAATT+CGG 0.353456 8_1:-9025003 None:intergenic
TTACATTCTATCATCTGTTT+CGG 0.356975 8_1:+9024189 Msa1176810:three_prime_UTR
TGGAGGAGGCGATGGTGTTA+TGG 0.360402 8_1:-9021088 None:intergenic
CTTATGTCAAAACCTTTGTT+AGG 0.362364 8_1:+9024920 Msa1176810:three_prime_UTR
GGTTTCTGCAAGGCTGTTGT+TGG 0.375497 8_1:+9024140 Msa1176810:three_prime_UTR
AAGTGATTGTTGCAGATTTG+TGG 0.395616 8_1:-9022543 None:intergenic
TGCATCCCGTATGATTTCTA+AGG 0.397018 8_1:-9025092 None:intergenic
TGATTGATATGTCCTTACTA+TGG 0.404239 8_1:+9025066 Msa1176810:three_prime_UTR
AAACGCCGATATGTATATAC+AGG 0.414217 8_1:-9024266 None:intergenic
AACAAAATATATATGGAATT+GGG 0.431675 8_1:+9022628 Msa1176810:CDS
TCATCTGAGAGTGTCACTTC+AGG 0.433664 8_1:+9024690 Msa1176810:three_prime_UTR
TCGCAAAATGTTGCAATGGA+TGG 0.472943 8_1:+9021396 Msa1176810:CDS
AAGAGAGAGGGGAGAAGTGG+TGG 0.476430 8_1:-9021129 None:intergenic
AGAGAGGGGAGAAGTGGTGG+AGG 0.479585 8_1:-9021126 None:intergenic
ATCGCCTCCTCCAAATCCTC+TGG 0.490776 8_1:+9021098 Msa1176810:CDS
ACAACTCCTTTCTCCAAAAG+AGG 0.491685 8_1:+9022707 Msa1176810:CDS
CGCAAAATGTTGCAATGGAT+GGG 0.493532 8_1:+9021397 Msa1176810:CDS
AGGCGATGGTGTTATGGTAG+AGG 0.494064 8_1:-9021082 None:intergenic
TATACAGGGCTACAAACAAC+AGG 0.497423 8_1:-9024251 None:intergenic
TGTAAGAGAGAGGGGAGAAG+TGG 0.502957 8_1:-9021132 None:intergenic
GACATGATCATGTGCTCGAA+GGG 0.510217 8_1:-9024548 None:intergenic
AACGATCCAAAATAATCTAA+AGG 0.512793 8_1:-9024310 None:intergenic
TGTTTGGTTTGTAAGAGAGA+GGG 0.514068 8_1:-9021141 None:intergenic
TTTAAACTTGATTTGCATGT+CGG 0.516080 8_1:-9024663 None:intergenic
GTGTTTGGTTTGTAAGAGAG+AGG 0.518181 8_1:-9021142 None:intergenic
CTTAAATGTCGGAGTTACAA+AGG 0.521121 8_1:-9024574 None:intergenic
AGCTTTGCTTGGTTTCTGCA+AGG 0.522263 8_1:+9024130 Msa1176810:three_prime_UTR
TTTGTGAATTAAGCTTTGCT+TGG 0.523241 8_1:+9024119 Msa1176810:three_prime_UTR
TAACAAGACAGCTGGTGGTA+TGG 0.523495 8_1:+9024096 Msa1176810:three_prime_UTR
TGGACTCCTTAGAAATCATA+CGG 0.523502 8_1:+9025086 Msa1176810:three_prime_UTR
GGTAGAACCAGAGGATTTGG+AGG 0.524437 8_1:-9021105 None:intergenic
ATCTTCAATAACAAGACAGC+TGG 0.531340 8_1:+9024088 Msa1176810:three_prime_UTR
GTAGAGGGTTCTGTAGAGAG+AGG 0.536430 8_1:-9021066 None:intergenic
GTTTGGTTTGTAAGAGAGAG+GGG 0.538206 8_1:-9021140 None:intergenic
ACTTGAAGAAAGTCACATGT+TGG 0.542285 8_1:+9022562 Msa1176810:CDS
CTTGAAGAAAGTCACATGTT+GGG 0.545996 8_1:+9022563 Msa1176810:CDS
TTCCTGATATTATTATGGCA+AGG 0.546222 8_1:+9025141 Msa1176810:three_prime_UTR
GTAAAATGCAACGTAGATGC+AGG 0.551145 8_1:+9022588 Msa1176810:CDS
AGAGGATTTGGAGGAGGCGA+TGG 0.551277 8_1:-9021096 None:intergenic
TAATTGGTCAATTGTGGAGT+GGG 0.552664 8_1:-9020967 None:intergenic
CATGCAAATCAAGTTTAAAG+TGG 0.552735 8_1:+9024667 Msa1176810:three_prime_UTR
GTAGCCCTGTATATACATAT+CGG 0.555152 8_1:+9024261 Msa1176810:three_prime_UTR
TTAAATGTCGGAGTTACAAA+GGG 0.556039 8_1:-9024573 None:intergenic
TTTCTAAGGAGTCCATAGTA+AGG 0.559933 8_1:-9025078 None:intergenic
GACGGTGCACACTGCACAAG+AGG 0.568565 8_1:+9020935 Msa1176810:five_prime_UTR
TATCAATTCACATGAAGCAA+TGG 0.573679 8_1:+9024599 Msa1176810:three_prime_UTR
AGAACCAGAGGATTTGGAGG+AGG 0.574524 8_1:-9021102 None:intergenic
TGACATGATCATGTGCTCGA+AGG 0.577214 8_1:-9024549 None:intergenic
GCAATCTAATTGGTCAATTG+TGG 0.577266 8_1:-9020973 None:intergenic
GGCGATGGTGTTATGGTAGA+GGG 0.577702 8_1:-9021081 None:intergenic
TCGCACGATGTAGCAATGGA+TGG 0.578431 8_1:+9022886 Msa1176810:exon
CGCACGATGTAGCAATGGAT+GGG 0.578591 8_1:+9022887 Msa1176810:exon
TTCAATAACAAGACAGCTGG+TGG 0.579642 8_1:+9024091 Msa1176810:three_prime_UTR
GGTGCACACTGCACAAGAGG+TGG 0.588133 8_1:+9020938 Msa1176810:five_prime_UTR
TTCATGCAGCAATAATTATG+TGG 0.590965 8_1:+9024632 Msa1176810:three_prime_UTR
CGTGAAAAGATGTAGAAGTG+AGG 0.591409 8_1:-9021020 None:intergenic
AACGTGATGCATGAAGTTGA+AGG 0.592616 8_1:+9024784 Msa1176810:three_prime_UTR
CCAAGAATGTTGGTAGGCGT+AGG 0.599131 8_1:+9024216 Msa1176810:three_prime_UTR
CACCTCGCACGATGTAGCAA+TGG 0.600042 8_1:+9022882 Msa1176810:exon
ACATGAAGCAATGGAACATG+TGG 0.600181 8_1:+9024608 Msa1176810:three_prime_UTR
AGGAAGAAAAGAAATCACAT+AGG 0.601143 8_1:-9021046 None:intergenic
CATCTCGCAAAATGTTGCAA+TGG 0.602901 8_1:+9021392 Msa1176810:CDS
AGTGGTGGAGGTAGAACCAG+AGG 0.608205 8_1:-9021114 None:intergenic
AACCGAATAATAAATTGAGA+AGG 0.609459 8_1:-9022744 None:intergenic
GTTGTTGGTATGTTTGCAGG+AGG 0.609963 8_1:+9024155 Msa1176810:three_prime_UTR
AAATTCAATACATTGTTGGA+CGG 0.610820 8_1:+9020917 None:intergenic
TTTCGGATGACCAAGAATGT+TGG 0.611670 8_1:+9024206 Msa1176810:three_prime_UTR
TTTGAGTCCTTGGGTGTCCG+TGG 0.624803 8_1:+9024440 Msa1176810:three_prime_UTR
GACAATGTTGCTATTGAGAG+TGG 0.626526 8_1:+9024714 Msa1176810:three_prime_UTR
ATCCATTGCTACATCGTGCG+AGG 0.628145 8_1:-9022884 None:intergenic
CAAAGTACAAGTCGTTGTGG+TGG 0.630808 8_1:+9024026 Msa1176810:three_prime_UTR
GGATGACCAAGAATGTTGGT+AGG 0.631174 8_1:+9024210 Msa1176810:three_prime_UTR
AAAATGTTGCAATGGATGGG+AGG 0.633582 8_1:+9021400 Msa1176810:CDS
AAAACAACCACGGACACCCA+AGG 0.638414 8_1:-9024447 None:intergenic
ACGATGTAGCAATGGATGGG+AGG 0.644267 8_1:+9022890 Msa1176810:exon
GGACTCCTTAGAAATCATAC+GGG 0.644350 8_1:+9025087 Msa1176810:three_prime_UTR
ATTCAAAGTACAAGTCGTTG+TGG 0.644376 8_1:+9024023 Msa1176810:three_prime_UTR
GAATTGATAATCTTAAATGT+CGG 0.646487 8_1:-9024585 None:intergenic
CTAATTGGTCAATTGTGGAG+TGG 0.651379 8_1:-9020968 None:intergenic
TGAATTCAACTCTGTCATTG+TGG 0.661215 8_1:-9022659 None:intergenic
CACACTGCACAAGAGGTGGA+CGG 0.666764 8_1:+9020942 Msa1176810:five_prime_UTR
ACATGATCATGTGCTCGAAG+GGG 0.676858 8_1:-9024547 None:intergenic
AACGCCGATATGTATATACA+GGG 0.683032 8_1:-9024265 None:intergenic
GTAAAATCGACACCTAACAA+AGG 0.692248 8_1:-9024932 None:intergenic
CATGAACCATAAAACAACCA+CGG 0.694607 8_1:-9024457 None:intergenic
AATGTTGCTATTGAGAGTGG+AGG 0.717923 8_1:+9024717 Msa1176810:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATTTATTATTATTATTC+AGG + chr8_1:9021212-9021231 Msa1176810:CDS 10.0%
!!! TTTTATCAACAAAATATATA+TGG + chr8_1:9022621-9022640 Msa1176810:CDS 10.0%
!! TATATATGATATGATAATAT+AGG + chr8_1:9023536-9023555 Msa1176810:intron 10.0%
!! ATAAAGAAGAAAATAAGAAT+GGG - chr8_1:9021768-9021787 None:intergenic 15.0%
!! AATTCAGTTCATATTAAATT+GGG + chr8_1:9022082-9022101 Msa1176810:intron 15.0%
!!! ATATTTTGTTGATAAAATAG+AGG - chr8_1:9022618-9022637 None:intergenic 15.0%
!! AACAAAATATATATGGAATT+GGG + chr8_1:9022628-9022647 Msa1176810:CDS 15.0%
!! AATTATTATTATACCATTCT+TGG + chr8_1:9022968-9022987 Msa1176810:intron 15.0%
!!! TTTTTGTTTTTTCTCTTTAT+TGG + chr8_1:9023006-9023025 Msa1176810:intron 15.0%
!!! TTTTGTTTTTTCTCTTTATT+GGG + chr8_1:9023007-9023026 Msa1176810:intron 15.0%
!! ATCATATCATATATAATGAT+AGG - chr8_1:9023531-9023550 None:intergenic 15.0%
!! TTTCATGAAAATTATAATTG+CGG + chr8_1:9023610-9023629 Msa1176810:intron 15.0%
!! TTATCATGTATGAAAATATA+AGG - chr8_1:9023940-9023959 None:intergenic 15.0%
!! TGAAATAGTATAACTATCAA+TGG - chr8_1:9021301-9021320 None:intergenic 20.0%
!!! ATACTATTTCAATTCGATTT+TGG + chr8_1:9021310-9021329 Msa1176810:intron 20.0%
!!! TATTCATCTACTTTTTTATC+AGG + chr8_1:9021507-9021526 Msa1176810:intron 20.0%
!! AATTTATATAGAGGATAACT+TGG - chr8_1:9021538-9021557 None:intergenic 20.0%
!! AAGTTATCCTCTATATAAAT+TGG + chr8_1:9021537-9021556 Msa1176810:intron 20.0%
!! CATAAAGAAGAAAATAAGAA+TGG - chr8_1:9021769-9021788 None:intergenic 20.0%
!! GAATTCAGTTCATATTAAAT+TGG + chr8_1:9022081-9022100 Msa1176810:intron 20.0%
!!! TTCAACTATTTTTAGAACAT+TGG + chr8_1:9022162-9022181 Msa1176810:intron 20.0%
!! CAACAAAATATATATGGAAT+TGG + chr8_1:9022627-9022646 Msa1176810:CDS 20.0%
!! TCAAATAGTATAACTAACAA+TGG - chr8_1:9022803-9022822 None:intergenic 20.0%
!! ATACTATTTGAATTCGATTT+CGG + chr8_1:9022812-9022831 Msa1176810:intron 20.0%
!!! CATGATTTTTCATTTTTTCT+CGG + chr8_1:9023227-9023246 Msa1176810:intron 20.0%
!! ACAAAATTGAACACAAAAAT+GGG - chr8_1:9023275-9023294 None:intergenic 20.0%
!! TATCATATATAATGATAGGA+AGG - chr8_1:9023527-9023546 None:intergenic 20.0%
!!! GAATTGATAATCTTAAATGT+CGG - chr8_1:9024588-9024607 None:intergenic 20.0%
!! TAGAGCTAAAATTCATAATT+CGG - chr8_1:9025006-9025025 None:intergenic 20.0%
!! GTTGTTTGTTTGTTTATAAT+AGG + chr8_1:9025028-9025047 Msa1176810:three_prime_UTR 20.0%
!!! TTTTGATTCGTTTCTACATT+CGG + chr8_1:9021359-9021378 Msa1176810:intron 25.0%
! TATTATTATCATCCCATTCT+TGG + chr8_1:9021480-9021499 Msa1176810:intron 25.0%
! TATCAAATTCGATTGCTTTA+TGG + chr8_1:9021614-9021633 Msa1176810:intron 25.0%
! GATTGTTCAAAAGAAGATAT+TGG + chr8_1:9021842-9021861 Msa1176810:intron 25.0%
!! CAAAAGAAGATATTGGTAAT+TGG + chr8_1:9021849-9021868 Msa1176810:intron 25.0%
! ACTAAAGCAGAAATTAAATG+AGG + chr8_1:9022005-9022024 Msa1176810:intron 25.0%
! CTAAAGCAGAAATTAAATGA+GGG + chr8_1:9022006-9022025 Msa1176810:intron 25.0%
! TATTAAATTGGGCTTGAATT+AGG + chr8_1:9022093-9022112 Msa1176810:intron 25.0%
!! TTTGAAGTGGAAAAATGAAA+AGG + chr8_1:9022443-9022462 Msa1176810:intron 25.0%
! TTGAAGTGGAAAAATGAAAA+GGG + chr8_1:9022444-9022463 Msa1176810:intron 25.0%
! GACCTTCTCAATTTATTATT+CGG + chr8_1:9022742-9022761 Msa1176810:CDS 25.0%
! AACCGAATAATAAATTGAGA+AGG - chr8_1:9022747-9022766 None:intergenic 25.0%
! AAGTTATCCTCTACATAAAT+TGG + chr8_1:9023037-9023056 Msa1176810:intron 25.0%
! CACAAAATTGAACACAAAAA+TGG - chr8_1:9023276-9023295 None:intergenic 25.0%
!!! ACATCATTTTGATTAAGAGT+TGG - chr8_1:9023723-9023742 None:intergenic 25.0%
!!! AATTTTTTAGATACATGAGG+AGG - chr8_1:9023758-9023777 None:intergenic 25.0%
!!! GTCAATTTTTTAGATACATG+AGG - chr8_1:9023761-9023780 None:intergenic 25.0%
!!! TACTTTTTTACTATGGGAAA+GGG - chr8_1:9023798-9023817 None:intergenic 25.0%
! GTTTCTCAAGATTGAATTTA+AGG + chr8_1:9023876-9023895 Msa1176810:intron 25.0%
! TTACATTCTATCATCTGTTT+CGG + chr8_1:9024189-9024208 Msa1176810:three_prime_UTR 25.0%
! AACGATCCAAAATAATCTAA+AGG - chr8_1:9024313-9024332 None:intergenic 25.0%
!!! TTTAAACTTGATTTGCATGT+CGG - chr8_1:9024666-9024685 None:intergenic 25.0%
! ACACTTTCCTGATATTATTA+TGG + chr8_1:9025136-9025155 Msa1176810:three_prime_UTR 25.0%
!!! GTTTTTTTTTCTTCGAATGA+AGG + chr8_1:9025198-9025217 Msa1176810:three_prime_UTR 25.0%
AATTGTCTGTGCAATCTAAT+TGG - chr8_1:9020986-9021005 None:intergenic 30.0%
AGGAAGAAAAGAAATCACAT+AGG - chr8_1:9021049-9021068 None:intergenic 30.0%
!! AATGAGTTGTTTCTTGTGTT+TGG - chr8_1:9021160-9021179 None:intergenic 30.0%
ACAACTCATTTCTCAAAAAG+AGG + chr8_1:9021170-9021189 Msa1176810:CDS 30.0%
TCAATCTCTCAAAAACACAA+TGG - chr8_1:9021273-9021292 None:intergenic 30.0%
TCAAAAGCAAAAGTCATCAA+TGG - chr8_1:9021345-9021364 None:intergenic 30.0%
GATGAATAAACACCAAGAAT+GGG - chr8_1:9021495-9021514 None:intergenic 30.0%
AGATGAATAAACACCAAGAA+TGG - chr8_1:9021496-9021515 None:intergenic 30.0%
AAGCATGCCAATTTATATAG+AGG - chr8_1:9021547-9021566 None:intergenic 30.0%
AATACTAAAGTGACTGTAGT+AGG + chr8_1:9022205-9022224 Msa1176810:intron 30.0%
ATTGTAGTGTGTCAATAGTT+TGG + chr8_1:9022289-9022308 Msa1176810:intron 30.0%
!! AGATTTGGATGATTTTGAAG+TGG + chr8_1:9022430-9022449 Msa1176810:intron 30.0%
! GAATCAGATTAATTTTCTGC+TGG + chr8_1:9022502-9022521 Msa1176810:intron 30.0%
!!! TTTTGGTTCGTTTCTACATT+AGG + chr8_1:9022849-9022868 Msa1176810:intron 30.0%
AGATGAATAAACACCAAGAA+TGG - chr8_1:9021496-9021515 None:intergenic 30.0%
AAGCATACCAATTTATGTAG+AGG - chr8_1:9023047-9023066 None:intergenic 30.0%
GTTGATATGATTGTAGTTTG+AGG + chr8_1:9023164-9023183 Msa1176810:intron 30.0%
!! TTTAGTTTGATATCCCTGTT+TGG + chr8_1:9023187-9023206 Msa1176810:intron 30.0%
ATGAAAAATCATGCTAGCAA+AGG - chr8_1:9023220-9023239 None:intergenic 30.0%
!! ATATGCGACTGAATTTGTTT+TGG + chr8_1:9023344-9023363 Msa1176810:intron 30.0%
GCATGAATTTGTGTATTTCA+TGG + chr8_1:9023366-9023385 Msa1176810:intron 30.0%
TGAATTTGTGTATTTCATGG+TGG + chr8_1:9023369-9023388 Msa1176810:intron 30.0%
! TCATGGTGGTAATTTTTGTA+AGG + chr8_1:9023383-9023402 Msa1176810:intron 30.0%
! CATGGTGGTAATTTTTGTAA+GGG + chr8_1:9023384-9023403 Msa1176810:intron 30.0%
! ATGGTGGTAATTTTTGTAAG+GGG + chr8_1:9023385-9023404 Msa1176810:intron 30.0%
CTCCAATGTAACAAATGTTA+CGG + chr8_1:9023441-9023460 Msa1176810:intron 30.0%
! AACCGTAACATTTGTTACAT+TGG - chr8_1:9023446-9023465 None:intergenic 30.0%
ATGATAGGAAGGATATTTGA+TGG - chr8_1:9023516-9023535 None:intergenic 30.0%
!!! CTACTTTTTTACTATGGGAA+AGG - chr8_1:9023799-9023818 None:intergenic 30.0%
!!! CAGAGCTACTTTTTTACTAT+GGG - chr8_1:9023804-9023823 None:intergenic 30.0%
!!! TCAGAGCTACTTTTTTACTA+TGG - chr8_1:9023805-9023824 None:intergenic 30.0%
TTTGTGAATTAAGCTTTGCT+TGG + chr8_1:9024119-9024138 Msa1176810:three_prime_UTR 30.0%
!! TATTTTGGATCGTTGTTTTC+TGG + chr8_1:9024319-9024338 Msa1176810:three_prime_UTR 30.0%
! CAGCAAAGAAAAACTTTTTG+CGG - chr8_1:9024407-9024426 None:intergenic 30.0%
!! TTTTTGTTTGTTTGAGTCCT+TGG + chr8_1:9024430-9024449 Msa1176810:three_prime_UTR 30.0%
!! TTTTGTTTGTTTGAGTCCTT+GGG + chr8_1:9024431-9024450 Msa1176810:three_prime_UTR 30.0%
!! TTGTTTTATGGTTCATGCAT+TGG + chr8_1:9024463-9024482 Msa1176810:three_prime_UTR 30.0%
TTAAATGTCGGAGTTACAAA+GGG - chr8_1:9024576-9024595 None:intergenic 30.0%
TATCAATTCACATGAAGCAA+TGG + chr8_1:9024599-9024618 Msa1176810:three_prime_UTR 30.0%
TTCATGCAGCAATAATTATG+TGG + chr8_1:9024632-9024651 Msa1176810:three_prime_UTR 30.0%
CATGCAAATCAAGTTTAAAG+TGG + chr8_1:9024667-9024686 Msa1176810:three_prime_UTR 30.0%
AACCATTAAGCAACCAAAAT+TGG + chr8_1:9024746-9024765 Msa1176810:three_prime_UTR 30.0%
!!! AACCAATTTTGGTTGCTTAA+TGG - chr8_1:9024751-9024770 None:intergenic 30.0%
! ATCTTCACATGAACCAATTT+TGG - chr8_1:9024762-9024781 None:intergenic 30.0%
CTTATGTCAAAACCTTTGTT+AGG + chr8_1:9024920-9024939 Msa1176810:three_prime_UTR 30.0%
TGATTGATATGTCCTTACTA+TGG + chr8_1:9025066-9025085 Msa1176810:three_prime_UTR 30.0%
TTCCTGATATTATTATGGCA+AGG + chr8_1:9025141-9025160 Msa1176810:three_prime_UTR 30.0%
TTCCTTGCCATAATAATATC+AGG - chr8_1:9025146-9025165 None:intergenic 30.0%
!!! TTTTTTTCTTCGAATGAAGG+TGG + chr8_1:9025201-9025220 Msa1176810:three_prime_UTR 30.0%
TAATTGGTCAATTGTGGAGT+GGG - chr8_1:9020970-9020989 None:intergenic 35.0%
GCAATCTAATTGGTCAATTG+TGG - chr8_1:9020976-9020995 None:intergenic 35.0%
!! TGTTTGGTTTGTAAGAGAGA+GGG - chr8_1:9021144-9021163 None:intergenic 35.0%
AAGTAGCTTTCAACATACCA+TGG - chr8_1:9021681-9021700 None:intergenic 35.0%
ACTCTTGAGTGTTCGATAAA+AGG + chr8_1:9021704-9021723 Msa1176810:intron 35.0%
CAGGTAGAACAAAAACAACT+AGG + chr8_1:9021941-9021960 Msa1176810:intron 35.0%
GTAGAACAAAAACAACTAGG+TGG + chr8_1:9021944-9021963 Msa1176810:intron 35.0%
ACAAAAACAACTAGGTGGAA+TGG + chr8_1:9021949-9021968 Msa1176810:intron 35.0%
!!! CTGCTTTAGTTTTTAAGACG+TGG - chr8_1:9021995-9022014 None:intergenic 35.0%
! AACTCGTGTCTACTATTTTG+AGG - chr8_1:9022241-9022260 None:intergenic 35.0%
ATGATGTTGCGATGTAGATT+TGG + chr8_1:9022415-9022434 Msa1176810:intron 35.0%
AAGTGATTGTTGCAGATTTG+TGG - chr8_1:9022546-9022565 None:intergenic 35.0%
ACTTGAAGAAAGTCACATGT+TGG + chr8_1:9022562-9022581 Msa1176810:CDS 35.0%
CTTGAAGAAAGTCACATGTT+GGG + chr8_1:9022563-9022582 Msa1176810:CDS 35.0%
TGAATTCAACTCTGTCATTG+TGG - chr8_1:9022662-9022681 None:intergenic 35.0%
!!! TCTTGTGTTTGGTTTTTGCT+CGG - chr8_1:9022686-9022705 None:intergenic 35.0%
!! AAGGAGTTGTTTCTTGTGTT+TGG - chr8_1:9022697-9022716 None:intergenic 35.0%
AGTATTTGCAATGCTCTCTA+TGG + chr8_1:9023099-9023118 Msa1176810:intron 35.0%
CAATGCTCTCTATGGAATTA+TGG + chr8_1:9023107-9023126 Msa1176810:intron 35.0%
CAAAGGCAAAAATCCAAACA+GGG - chr8_1:9023203-9023222 None:intergenic 35.0%
! CGTAACATTTGTTACATTGG+AGG - chr8_1:9023443-9023462 None:intergenic 35.0%
ACTATACTCGATCACAAGTT+AGG + chr8_1:9023830-9023849 Msa1176810:intron 35.0%
ATTCAAAGTACAAGTCGTTG+TGG + chr8_1:9024023-9024042 Msa1176810:three_prime_UTR 35.0%
! CTACAACTTTTGTCTAGCAA+AGG + chr8_1:9024061-9024080 Msa1176810:three_prime_UTR 35.0%
ATCTTCAATAACAAGACAGC+TGG + chr8_1:9024088-9024107 Msa1176810:three_prime_UTR 35.0%
GTAGCCCTGTATATACATAT+CGG + chr8_1:9024261-9024280 Msa1176810:three_prime_UTR 35.0%
AACGCCGATATGTATATACA+GGG - chr8_1:9024268-9024287 None:intergenic 35.0%
AAACGCCGATATGTATATAC+AGG - chr8_1:9024269-9024288 None:intergenic 35.0%
! AGCGGTCCTTTAGATTATTT+TGG + chr8_1:9024304-9024323 Msa1176810:three_prime_UTR 35.0%
!!! TGCTGCTAATTTTCTGGTTT+TGG + chr8_1:9024368-9024387 Msa1176810:three_prime_UTR 35.0%
!!! TGCTAATTTTCTGGTTTTGG+TGG + chr8_1:9024371-9024390 Msa1176810:three_prime_UTR 35.0%
CATGAACCATAAAACAACCA+CGG - chr8_1:9024460-9024479 None:intergenic 35.0%
!!! CAACCTGTTTTTAGTCTGTA+TGG + chr8_1:9024494-9024513 Msa1176810:three_prime_UTR 35.0%
ATGCCATACAGACTAAAAAC+AGG - chr8_1:9024500-9024519 None:intergenic 35.0%
!!! GTTTTTAGTCTGTATGGCAT+TGG + chr8_1:9024500-9024519 Msa1176810:three_prime_UTR 35.0%
CTTAAATGTCGGAGTTACAA+AGG - chr8_1:9024577-9024596 None:intergenic 35.0%
GTAAAATCGACACCTAACAA+AGG - chr8_1:9024935-9024954 None:intergenic 35.0%
!!! TTGTTAGGTGTCGATTTTAC+TGG + chr8_1:9024935-9024954 Msa1176810:three_prime_UTR 35.0%
! TTTCTAAGGAGTCCATAGTA+AGG - chr8_1:9025081-9025100 None:intergenic 35.0%
TGGACTCCTTAGAAATCATA+CGG + chr8_1:9025086-9025105 Msa1176810:three_prime_UTR 35.0%
CTAATTGGTCAATTGTGGAG+TGG - chr8_1:9020971-9020990 None:intergenic 40.0%
CGTGAAAAGATGTAGAAGTG+AGG - chr8_1:9021023-9021042 None:intergenic 40.0%
!! GTTTGGTTTGTAAGAGAGAG+GGG - chr8_1:9021143-9021162 None:intergenic 40.0%
!! GTGTTTGGTTTGTAAGAGAG+AGG - chr8_1:9021145-9021164 None:intergenic 40.0%
CATCTCGCAAAATGTTGCAA+TGG + chr8_1:9021392-9021411 Msa1176810:CDS 40.0%
TCGCAAAATGTTGCAATGGA+TGG + chr8_1:9021396-9021415 Msa1176810:CDS 40.0%
CGCAAAATGTTGCAATGGAT+GGG + chr8_1:9021397-9021416 Msa1176810:CDS 40.0%
AAAATGTTGCAATGGATGGG+AGG + chr8_1:9021400-9021419 Msa1176810:CDS 40.0%
AGTATGAGAGAGAGATTCCA+TGG + chr8_1:9021661-9021680 Msa1176810:intron 40.0%
AAATCGTTACTGTCAAGTGC+AGG + chr8_1:9021916-9021935 Msa1176810:intron 40.0%
GACGTGGTAATAAGCTTTCT+AGG - chr8_1:9021979-9021998 None:intergenic 40.0%
!!! GTCGCAATGCTGATTTTCTT+TGG + chr8_1:9022360-9022379 Msa1176810:intron 40.0%
! ATTTTCTTTGGTCTGCTCAC+AGG + chr8_1:9022372-9022391 Msa1176810:intron 40.0%
GCTGGAAATTCACCAAATTC+AGG + chr8_1:9022520-9022539 Msa1176810:intron 40.0%
GTAAAATGCAACGTAGATGC+AGG + chr8_1:9022588-9022607 Msa1176810:CDS 40.0%
ACAACTCCTTTCTCCAAAAG+AGG + chr8_1:9022707-9022726 Msa1176810:CDS 40.0%
!!! CGGTTGATGATTTCTGCTTT+TGG + chr8_1:9022832-9022851 Msa1176810:intron 40.0%
GCAAAGGCAAAAATCCAAAC+AGG - chr8_1:9023204-9023223 None:intergenic 40.0%
! AAGGGGCATATGTTACAAGT+AGG + chr8_1:9023402-9023421 Msa1176810:intron 40.0%
! AGGGGCATATGTTACAAGTA+GGG + chr8_1:9023403-9023422 Msa1176810:intron 40.0%
TTCAATAACAAGACAGCTGG+TGG + chr8_1:9024091-9024110 Msa1176810:three_prime_UTR 40.0%
! TATGTTTGCAGGAGGTTTTC+AGG + chr8_1:9024163-9024182 Msa1176810:three_prime_UTR 40.0%
TTTCGGATGACCAAGAATGT+TGG + chr8_1:9024206-9024225 Msa1176810:three_prime_UTR 40.0%
TATACAGGGCTACAAACAAC+AGG - chr8_1:9024254-9024273 None:intergenic 40.0%
! CATATCGGCGTTTTCTTCTT+TGG + chr8_1:9024276-9024295 Msa1176810:three_prime_UTR 40.0%
! TTTTCTTCTTTGGTCACGAG+CGG + chr8_1:9024286-9024305 Msa1176810:three_prime_UTR 40.0%
!!! GGATCGTTGTTTTCTGGTTT+CGG + chr8_1:9024325-9024344 Msa1176810:three_prime_UTR 40.0%
! CTGCTATGCTGCTAATTTTC+TGG + chr8_1:9024362-9024381 Msa1176810:three_prime_UTR 40.0%
!!! AGTCTGTATGGCATTGGTTT+TGG + chr8_1:9024506-9024525 Msa1176810:three_prime_UTR 40.0%
ACATGAAGCAATGGAACATG+TGG + chr8_1:9024608-9024627 Msa1176810:three_prime_UTR 40.0%
! GACAATGTTGCTATTGAGAG+TGG + chr8_1:9024714-9024733 Msa1176810:three_prime_UTR 40.0%
! AATGTTGCTATTGAGAGTGG+AGG + chr8_1:9024717-9024736 Msa1176810:three_prime_UTR 40.0%
AACGTGATGCATGAAGTTGA+AGG + chr8_1:9024784-9024803 Msa1176810:three_prime_UTR 40.0%
GGACTCCTTAGAAATCATAC+GGG + chr8_1:9025087-9025106 Msa1176810:three_prime_UTR 40.0%
TGCATCCCGTATGATTTCTA+AGG - chr8_1:9025095-9025114 None:intergenic 40.0%
GGATCACTAGAACTTACACC+AGG + chr8_1:9021725-9021744 Msa1176810:intron 45.0%
CTTACACCAGGCTTTCTTGA+TGG + chr8_1:9021737-9021756 Msa1176810:intron 45.0%
GACACGAGTTCTATGTTGTG+TGG + chr8_1:9022251-9022270 Msa1176810:intron 45.0%
AAGGGTTTGTCAAAGCTGCA+AGG + chr8_1:9022462-9022481 Msa1176810:intron 45.0%
AGGGTTTGTCAAAGCTGCAA+GGG + chr8_1:9022463-9022482 Msa1176810:intron 45.0%
GCAGATTTGTGGCCTGAATT+TGG - chr8_1:9022535-9022554 None:intergenic 45.0%
!! CTTCTGCCTCTTTTGGAGAA+AGG - chr8_1:9022716-9022735 None:intergenic 45.0%
! CTCATAGCTTCTGCCTCTTT+TGG - chr8_1:9021186-9021205 None:intergenic 45.0%
CAAAGTACAAGTCGTTGTGG+TGG + chr8_1:9024026-9024045 Msa1176810:three_prime_UTR 45.0%
TAACAAGACAGCTGGTGGTA+TGG + chr8_1:9024096-9024115 Msa1176810:three_prime_UTR 45.0%
AGCTTTGCTTGGTTTCTGCA+AGG + chr8_1:9024130-9024149 Msa1176810:three_prime_UTR 45.0%
GCTGTTGTTGGTATGTTTGC+AGG + chr8_1:9024152-9024171 Msa1176810:three_prime_UTR 45.0%
GTTGTTGGTATGTTTGCAGG+AGG + chr8_1:9024155-9024174 Msa1176810:three_prime_UTR 45.0%
GGATGACCAAGAATGTTGGT+AGG + chr8_1:9024210-9024229 Msa1176810:three_prime_UTR 45.0%
!!! GCATTGGTTTTGGCTTTGTG+TGG + chr8_1:9024516-9024535 Msa1176810:three_prime_UTR 45.0%
ACATGATCATGTGCTCGAAG+GGG - chr8_1:9024550-9024569 None:intergenic 45.0%
GACATGATCATGTGCTCGAA+GGG - chr8_1:9024551-9024570 None:intergenic 45.0%
TGACATGATCATGTGCTCGA+AGG - chr8_1:9024552-9024571 None:intergenic 45.0%
!! TCATCTGAGAGTGTCACTTC+AGG + chr8_1:9024690-9024709 Msa1176810:three_prime_UTR 45.0%
GTAGAGGGTTCTGTAGAGAG+AGG - chr8_1:9021069-9021088 None:intergenic 50.0%
!! GGCGATGGTGTTATGGTAGA+GGG - chr8_1:9021084-9021103 None:intergenic 50.0%
!! AGGCGATGGTGTTATGGTAG+AGG - chr8_1:9021085-9021104 None:intergenic 50.0%
AGAACCAGAGGATTTGGAGG+AGG - chr8_1:9021105-9021124 None:intergenic 50.0%
GGTAGAACCAGAGGATTTGG+AGG - chr8_1:9021108-9021127 None:intergenic 50.0%
GGAGGTAGAACCAGAGGATT+TGG - chr8_1:9021111-9021130 None:intergenic 50.0%
TGTAAGAGAGAGGGGAGAAG+TGG - chr8_1:9021135-9021154 None:intergenic 50.0%
! ATGGATGGGAGGATCGATTC+TGG + chr8_1:9021411-9021430 Msa1176810:CDS 50.0%
GACTTGCCATCAAGAAAGCC+TGG - chr8_1:9021746-9021765 None:intergenic 50.0%
TTACTGTCAAGTGCAGGCAC+AGG + chr8_1:9021922-9021941 Msa1176810:intron 50.0%
AAGCTGCAAGGGACATTCTG+AGG + chr8_1:9022474-9022493 Msa1176810:intron 50.0%
! CAAGGGACATTCTGAGGAGT+TGG + chr8_1:9022480-9022499 Msa1176810:intron 50.0%
ATCCATTGCTACATCGTGCG+AGG - chr8_1:9022887-9022906 None:intergenic 50.0%
TCGCACGATGTAGCAATGGA+TGG + chr8_1:9022886-9022905 Msa1176810:exon 50.0%
CGCACGATGTAGCAATGGAT+GGG + chr8_1:9022887-9022906 Msa1176810:exon 50.0%
ACGATGTAGCAATGGATGGG+AGG + chr8_1:9022890-9022909 Msa1176810:exon 50.0%
! ATGGATGGGAGGATCGATTC+TGG + chr8_1:9021411-9021430 Msa1176810:CDS 50.0%
GGTTTCTGCAAGGCTGTTGT+TGG + chr8_1:9024140-9024159 Msa1176810:three_prime_UTR 50.0%
CCTACGCCTACCAACATTCT+TGG - chr8_1:9024219-9024238 None:intergenic 50.0%
! CCAAGAATGTTGGTAGGCGT+AGG + chr8_1:9024216-9024235 Msa1176810:three_prime_UTR 50.0%
AAAACAACCACGGACACCCA+AGG - chr8_1:9024450-9024469 None:intergenic 50.0%
!! GGGTGTCCGTGGTTGTTTTA+TGG + chr8_1:9024451-9024470 Msa1176810:three_prime_UTR 50.0%
CACACTGCACAAGAGGTGGA+CGG + chr8_1:9020942-9020961 Msa1176810:five_prime_UTR 55.0%
! TGGAGGAGGCGATGGTGTTA+TGG - chr8_1:9021091-9021110 None:intergenic 55.0%
AGAGGATTTGGAGGAGGCGA+TGG - chr8_1:9021099-9021118 None:intergenic 55.0%
ATCGCCTCCTCCAAATCCTC+TGG + chr8_1:9021098-9021117 Msa1176810:CDS 55.0%
! AGTGGTGGAGGTAGAACCAG+AGG - chr8_1:9021117-9021136 None:intergenic 55.0%
AAGAGAGAGGGGAGAAGTGG+TGG - chr8_1:9021132-9021151 None:intergenic 55.0%
CACCTCGCACGATGTAGCAA+TGG + chr8_1:9022882-9022901 Msa1176810:exon 55.0%
TTTGAGTCCTTGGGTGTCCG+TGG + chr8_1:9024440-9024459 Msa1176810:three_prime_UTR 55.0%
GACGGTGCACACTGCACAAG+AGG + chr8_1:9020935-9020954 Msa1176810:five_prime_UTR 60.0%
GGTGCACACTGCACAAGAGG+TGG + chr8_1:9020938-9020957 Msa1176810:five_prime_UTR 60.0%
AGAGAGGGGAGAAGTGGTGG+AGG - chr8_1:9021129-9021148 None:intergenic 60.0%
TGGTCTGCTCACAGGCTTGC+AGG + chr8_1:9022380-9022399 Msa1176810:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 9020931 9025305 9020931 ID=Msa1176810;Name=Msa1176810
chr8_1 mRNA 9020931 9025305 9020931 ID=Msa1176810-mRNA-1;Parent=Msa1176810;Name=Msa1176810-mRNA-1;_AED=0.12;_eAED=0.12;_QI=81|0.6|0.66|1|0.2|0.33|6|1319|184
chr8_1 exon 9020931 9021233 9020931 ID=Msa1176810-mRNA-1:exon:3040;Parent=Msa1176810-mRNA-1
chr8_1 exon 9021388 9021444 9021388 ID=Msa1176810-mRNA-1:exon:3041;Parent=Msa1176810-mRNA-1
chr8_1 exon 9022402 9022427 9022402 ID=Msa1176810-mRNA-1:exon:3042;Parent=Msa1176810-mRNA-1
chr8_1 exon 9022542 9022763 9022542 ID=Msa1176810-mRNA-1:exon:3043;Parent=Msa1176810-mRNA-1
chr8_1 exon 9022871 9022934 9022871 ID=Msa1176810-mRNA-1:exon:3044;Parent=Msa1176810-mRNA-1
chr8_1 exon 9024023 9025305 9024023 ID=Msa1176810-mRNA-1:exon:3045;Parent=Msa1176810-mRNA-1
chr8_1 five_prime_UTR 9020931 9021011 9020931 ID=Msa1176810-mRNA-1:five_prime_utr;Parent=Msa1176810-mRNA-1
chr8_1 CDS 9021012 9021233 9021012 ID=Msa1176810-mRNA-1:cds;Parent=Msa1176810-mRNA-1
chr8_1 CDS 9021388 9021444 9021388 ID=Msa1176810-mRNA-1:cds;Parent=Msa1176810-mRNA-1
chr8_1 CDS 9022402 9022427 9022402 ID=Msa1176810-mRNA-1:cds;Parent=Msa1176810-mRNA-1
chr8_1 CDS 9022542 9022763 9022542 ID=Msa1176810-mRNA-1:cds;Parent=Msa1176810-mRNA-1
chr8_1 CDS 9022871 9022898 9022871 ID=Msa1176810-mRNA-1:cds;Parent=Msa1176810-mRNA-1
chr8_1 three_prime_UTR 9022899 9022934 9022899 ID=Msa1176810-mRNA-1:three_prime_utr;Parent=Msa1176810-mRNA-1
chr8_1 three_prime_UTR 9024023 9025305 9024023 ID=Msa1176810-mRNA-1:three_prime_utr;Parent=Msa1176810-mRNA-1
Gene Sequence

>Msa1176810

ATGATACTCCTCACTTCTACATCTTTTCACGTTTCCTATGTGATTTCTTTTCTTCCTCTCTCTACAGAACCCTCTACCATAACACCATCGCCTCCTCCAAATCCTCTGGTTCTACCTCCACCACTTCTCCCCTCTCTCTTACAAACCAAACACAAGAAACAACTCATTTCTCAAAAAGAGGCAGAAGCTATGAGACTTTCTCAATTTATTATTATTATTCAGATCGCATCTCGCAAAATGTTGCAATGGATGGGAGGATCGATTCTGGAAGTTAATGCTGAACAGAAATGCTATGATGTTGCGATGCCACAAATCTGCAACAATCACTTGAAGAAAGTCACATGTTGGGTCGTAAAATGCAACGTAGATGCAGGCACACCTCTATTTTATCAACAAAATATATATGGAATTGGGATATGCATCCACAATGACAGAGTTGAATTCATCCGAGCAAAAACCAAACACAAGAAACAACTCCTTTCTCCAAAAGAGGCAGAAGCTATGAGACCTTCTCAATTTATTATTCGGTTTCAGATCGCACCTCGCACGATGTAG
Protein sequence

>Msa1176810

MILLTSTSFHVSYVISFLPLSTEPSTITPSPPPNPLVLPPPLLPSLLQTKHKKQLISQKEAEAMRLSQFIIIIQIASRKMLQWMGGSILEVNAEQKCYDVAMPQICNNHLKKVTCWVVKCNVDAGTPLFYQQNIYGIGICIHNDRVEFIRAKTKHKKQLLSPKEAEAMRPSQFIIRFQIAPRTM*