AlfalfaGEDB | Msa1183510

M. sativa cultivar zhongmu-4 / Msa1183510

KEGG: K11340
GO: GO:0000003 GO:0003006 GO:0003674 GO:0005198 GO:0005200 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006325 GO:0006996 GO:0007010 GO:0007275 GO:0007276 GO:0008150 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009791 GO:0009987 GO:0010228 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0048229 GO:0048232 GO:0048235 GO:0048571 GO:0048573 GO:0048574 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0051276 GO:0051704 GO:0055046 GO:0061458 GO:0070013 GO:0071840
PFAM: PF00022.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183510	sp\|Q84M92\|ARP4_ARATH	78.125	448	88	4	1	445	1	441	0.0	747

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183510	G7KBM8	99.326	445	3	0	1	445	1	445	0.0	914

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1183510	Msa1271860	0.866242	3.290335e-65	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183510	MtrunA17_Chr5g0427591	99.101	445	3	1	1	445	1	444	0.0	907
Msa1183510	MtrunA17_Chr8g0361001	99.101	445	3	1	1	445	1	444	0.0	907
Msa1183510	MtrunA17_Chr1g0175391	38.031	447	203	12	1	444	1	376	2.16e-93	286
Msa1183510	MtrunA17_Chr2g0328091	37.360	447	206	11	1	444	1	376	6.03e-93	285
Msa1183510	MtrunA17_Chr6g0448881	37.584	447	205	12	1	444	1	376	3.62e-92	283
Msa1183510	MtrunA17_Chr7g0258721	37.136	447	207	12	1	444	1	376	3.27e-91	281
Msa1183510	MtrunA17_Chr3g0129641	37.360	447	206	12	1	444	1	376	1.34e-90	280
Msa1183510	MtrunA17_Chr7g0223901	37.360	447	206	12	1	444	1	376	1.49e-90	279
Msa1183510	MtrunA17_Chr2g0278591	36.465	447	210	12	1	444	1	376	9.15e-90	277
Msa1183510	MtrunA17_Chr8g0336071	97.938	97	2	0	131	227	1	97	1.75e-61	194

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183510	AT1G18450.1	78.125	448	88	4	1	445	1	441	0.0	747
Msa1183510	AT3G53750.1	37.360	447	206	11	1	444	1	376	1.68e-93	287
Msa1183510	AT3G53750.2	37.360	447	206	11	1	444	1	376	1.68e-93	287
Msa1183510	AT2G37620.1	37.360	447	206	11	1	444	1	376	1.68e-93	287
Msa1183510	AT2G37620.3	37.360	447	206	11	1	444	1	376	1.68e-93	287
Msa1183510	AT2G37620.2	37.360	447	206	11	1	444	1	376	1.68e-93	287
Msa1183510	AT2G37620.4	37.360	447	206	11	1	444	1	376	1.68e-93	287
Msa1183510	AT3G12110.1	38.031	447	203	12	1	444	1	376	5.17e-93	286
Msa1183510	AT5G09810.1	38.255	447	202	12	1	444	1	376	1.27e-92	285
Msa1183510	AT1G49240.1	37.808	447	204	10	1	444	1	376	5.04e-92	283

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Find 157 sgRNAs with CRISPR-Local

Find 1013 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTCTACGGTAGCCCA+AGG	0.674998	8_1:-18757317	None:intergenic
AAACCATCATATGGAGTATC+TGG	0.319612	8_1:-18764519	None:intergenic
AAATCGCTCCATCAGTTCGT+GGG	0.616076	8_1:+18766354	Msa1183510:CDS
AACAATATTAGAAATGTTGA+TGG	0.388533	8_1:+18757250	Msa1183510:CDS
AAGCTACAAGCTCTACTCCC+AGG	0.608537	8_1:+18764384	Msa1183510:CDS
AATACGCCCTGGAGAGTTTC+AGG	0.253290	8_1:+18763062	Msa1183510:CDS
AATCTGACAGACTGTAGACT+TGG	0.573313	8_1:+18764342	Msa1183510:intron
ACAATTAGACAGAGATCCAA+TGG	0.531915	8_1:-18769873	None:intergenic
ACAGAAGGAAACACAGCTTT+GGG	0.447748	8_1:-18756191	None:intergenic
ACAGTTGCACCGGTACATGA+TGG	0.606325	8_1:+18761602	Msa1183510:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAGCTATAGTAATTGATC+TGG	+	chr8_1:18756127-18756146	Msa1183510:CDS	30.0%
TCAGCTATAGTAATTGATCT+GGG	+	chr8_1:18756128-18756147	Msa1183510:CDS	30.0%
TTGTGCTTAATTCACTTTGT+TGG	-	chr8_1:18756529-18756548	None:intergenic	30.0%
CAACATTATAGAGAGAATTC+GGG	+	chr8_1:18756610-18756629	Msa1183510:intron	30.0%
TCTTTCTTAGTAGTTTCTCT+AGG	-	chr8_1:18756751-18756770	None:intergenic	30.0%
GATAGTGTAATTAGGTGTAA+TGG	+	chr8_1:18757041-18757060	Msa1183510:intron	30.0%
TTACCTCCATAAAATCTCTA+CGG	-	chr8_1:18757330-18757349	None:intergenic	30.0%
TCAAGAAGAACGTTACAAAA+GGG	-	chr8_1:18757353-18757372	None:intergenic	30.0%
TTCAAGAAGAACGTTACAAA+AGG	-	chr8_1:18757354-18757373	None:intergenic	30.0%
AGCTTAATTTATATGTGGCT+AGG	-	chr8_1:18757742-18757761	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	18755838	18770247	18755838	ID=Msa1183510;Name=Msa1183510
chr8_1	mRNA	18755838	18770247	18755838	ID=Msa1183510-mRNA-1;Parent=Msa1183510;Name=Msa1183510-mRNA-1;_AED=0.13;_eAED=0.13;_QI=180\|0.94\|0.95\|1\|0.89\|0.9\|20\|455\|445
chr8_1	exon	18755838	18756030	18755838	ID=Msa1183510-mRNA-1:exon:7188;Parent=Msa1183510-mRNA-1
chr8_1	exon	18756120	18756211	18756120	ID=Msa1183510-mRNA-1:exon:7189;Parent=Msa1183510-mRNA-1
chr8_1	exon	18757172	18757345	18757172	ID=Msa1183510-mRNA-1:exon:7190;Parent=Msa1183510-mRNA-1
chr8_1	exon	18757446	18757519	18757446	ID=Msa1183510-mRNA-1:exon:7191;Parent=Msa1183510-mRNA-1
chr8_1	exon	18759996	18760079	18759996	ID=Msa1183510-mRNA-1:exon:7192;Parent=Msa1183510-mRNA-1
chr8_1	exon	18760168	18760228	18760168	ID=Msa1183510-mRNA-1:exon:7193;Parent=Msa1183510-mRNA-1
chr8_1	exon	18760330	18760379	18760330	ID=Msa1183510-mRNA-1:exon:7194;Parent=Msa1183510-mRNA-1
chr8_1	exon	18761586	18761640	18761586	ID=Msa1183510-mRNA-1:exon:7195;Parent=Msa1183510-mRNA-1
chr8_1	exon	18762852	18762929	18762852	ID=Msa1183510-mRNA-1:exon:7196;Parent=Msa1183510-mRNA-1
chr8_1	exon	18763030	18763083	18763030	ID=Msa1183510-mRNA-1:exon:7197;Parent=Msa1183510-mRNA-1
chr8_1	exon	18764352	18764405	18764352	ID=Msa1183510-mRNA-1:exon:7198;Parent=Msa1183510-mRNA-1
chr8_1	exon	18764477	18764537	18764477	ID=Msa1183510-mRNA-1:exon:7199;Parent=Msa1183510-mRNA-1
chr8_1	exon	18764858	18764909	18764858	ID=Msa1183510-mRNA-1:exon:7200;Parent=Msa1183510-mRNA-1
chr8_1	exon	18765096	18765162	18765096	ID=Msa1183510-mRNA-1:exon:7201;Parent=Msa1183510-mRNA-1
chr8_1	exon	18766326	18766388	18766326	ID=Msa1183510-mRNA-1:exon:7202;Parent=Msa1183510-mRNA-1
chr8_1	exon	18766714	18766776	18766714	ID=Msa1183510-mRNA-1:exon:7203;Parent=Msa1183510-mRNA-1
chr8_1	exon	18767294	18767356	18767294	ID=Msa1183510-mRNA-1:exon:7204;Parent=Msa1183510-mRNA-1
chr8_1	exon	18768676	18768740	18768676	ID=Msa1183510-mRNA-1:exon:7205;Parent=Msa1183510-mRNA-1
chr8_1	exon	18769546	18769614	18769546	ID=Msa1183510-mRNA-1:exon:7206;Parent=Msa1183510-mRNA-1
chr8_1	exon	18769747	18770247	18769747	ID=Msa1183510-mRNA-1:exon:7207;Parent=Msa1183510-mRNA-1
chr8_1	five_prime_UTR	18755838	18756017	18755838	ID=Msa1183510-mRNA-1:five_prime_utr;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18756018	18756030	18756018	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18756120	18756211	18756120	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18757172	18757345	18757172	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18757446	18757519	18757446	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18759996	18760079	18759996	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18760168	18760228	18760168	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18760330	18760379	18760330	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18761586	18761640	18761586	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18762852	18762929	18762852	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18763030	18763083	18763030	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18764352	18764405	18764352	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18764477	18764537	18764477	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18764858	18764909	18764858	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18765096	18765162	18765096	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18766326	18766388	18766326	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18766714	18766776	18766714	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18767294	18767356	18767294	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18768676	18768740	18768676	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18769546	18769614	18769546	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	CDS	18769747	18769792	18769747	ID=Msa1183510-mRNA-1:cds;Parent=Msa1183510-mRNA-1
chr8_1	three_prime_UTR	18769793	18770247	18769793	ID=Msa1183510-mRNA-1:three_prime_utr;Parent=Msa1183510-mRNA-1

Gene Sequence

>Msa1183510

ATGTACGGTGGTGATGAAGTTTCAGCTATAGTAATTGATCTGGGTTCACATACTTGTAAAGCTGGTTATGCTGGTGAAGATGCTCCCAAAGCTGTGTTTCCTTCTGTTGTTGGAGCGATTGATCAAATGGATGTTGATGAATCAGACGAGGCTGAAAAGAACTCTGGCTCTGGCGAATCAAAGAACAATATTAGAAATGTTGATGGTGATAAAGCCAAGGGAAAGAGAAAATTATATGTAGGATCTCAGTCCTTGGGCTACCGTAGAGATTTTATGGAGGTGGTGTCTCCATTAAAGGATGGAATTGTTGCTGACTGGGATATTGTTGACAGCATTTGGGATCATGCCTTCAGTAGGGAGTGCCTATTGATTGATCCTAAAGAGCATCCCATGCTCCTTGCAGAGCCTTCTTCTAATGCTCAACAACAGAGAGAGAGGGCAGCAGAGCTTATGTTTGAGAAGTACAAAGCACCTGCATTATTTTTGGCAAAAAATGCTGTTTTGACTTCTTTTGCATCAGGGCGTGCCACATCATTAGTTGTTGATTGTGGTGGAGGATCAACTACAGTTGCACCGGTACATGATGGCTATGTTCTTCAGAAGGCTGTGGCAACGTCTCCAATTGGAGGAGAATTTCTTACAGATTGCTTGATGAAAAGCCTGGAAAGCAAGGGTATTGCGATGAAACCTCGGTATTCCTTTAGGAGAAAGGAAATACGCCCTGGAGAGTTTCAGACTGTAGACTTGGATTTTCCTAATACATCTGAAAGCTACAAGCTCTACTCCCAGAGGGTGATTGCTAGTGATATCAAGGAATGTGTATGCCGTGCTCCAGATACTCCATATGATGAGAGTGCATATTCAAACATCCCAACGACATCGTATGAGCTACCTGATGGCCAGACAATTGAAATTGGAGCTGACAGATTTAAGATTCCAGATGTTCTTTTCAATCCATCCCTGGCTCAGTCAATTCCGGGCATGGAGAGCTTTGCAGAAATCGCTCCATCAGTTCGTGGGCTGCCTCAAATGGTTATCGAAAGCATTAATAAGTGTGATGTAGACATTCGAAGAGAATTATTTAGTAGCATACTGCTTGCTGGTGGTACGGCTTCAATGCAACAACTGAAGGAACGCCTTGAGAAAGACTTGCTAGAGGAATCCCCTCAGGCTGCTAGAGTAAAGGTATTGGCCAGTGGAAATGCTACTGAAAGGAGGTTTAGTGTCTGGATTGGAGGCAGTATATTGGCTTCCCTTGGTTCCTTCCAACAAATGTGGTTCTCCAAATCCGAATATGAAGAGCATGGAGCTTCATATATCCAAAGGAAGTGTCCTTGA

Protein sequence

>Msa1183510

MYGGDEVSAIVIDLGSHTCKAGYAGEDAPKAVFPSVVGAIDQMDVDESDEAEKNSGSGESKNNIRNVDGDKAKGKRKLYVGSQSLGYRRDFMEVVSPLKDGIVADWDIVDSIWDHAFSRECLLIDPKEHPMLLAEPSSNAQQQRERAAELMFEKYKAPALFLAKNAVLTSFASGRATSLVVDCGGGSTTVAPVHDGYVLQKAVATSPIGGEFLTDCLMKSLESKGIAMKPRYSFRRKEIRPGEFQTVDLDFPNTSESYKLYSQRVIASDIKECVCRAPDTPYDESAYSNIPTTSYELPDGQTIEIGADRFKIPDVLFNPSLAQSIPGMESFAEIAPSVRGLPQMVIESINKCDVDIRRELFSSILLAGGTASMQQLKERLEKDLLEESPQAARVKVLASGNATERRFSVWIGGSILASLGSFQQMWFSKSEYEEHGASYIQRKCP*