AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1185010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGCATCATTAGGAATTT+TGG 0.125595 8_1:+21478254 Msa1185010:three_prime_UTR
TTTCTATTACAGTCTGTTAA+AGG 0.222179 8_1:+21478461 Msa1185010:three_prime_UTR
TTGGATCTTCTTGCATCATT+AGG 0.284219 8_1:+21478246 Msa1185010:three_prime_UTR
AACTCGCACTCGTTGCTCTT+TGG 0.301276 8_1:+21478227 Msa1185010:three_prime_UTR
AAACATTCAGATAAATGTTA+TGG 0.381557 8_1:+21478091 Msa1185010:three_prime_UTR
ACGAGTGCGAGTTAAATAAA+AGG 0.384482 8_1:-21478217 None:intergenic
AAATTCAATGTTGCCCTTCA+CGG 0.440501 8_1:+21478036 Msa1185010:CDS
GGCACAGTGGAATGTGGTTC+AGG 0.440587 8_1:+21475635 Msa1185010:CDS
AATTCAATGTTGCCCTTCAC+GGG 0.451120 8_1:+21478037 Msa1185010:CDS
TTCAGGACGGCTTGGAGTGT+TGG 0.487719 8_1:+21475652 Msa1185010:CDS
TTTGGATTTGCAGGAGCTGA+AGG 0.529082 8_1:+21477996 Msa1185010:intron
ATCACTCAACCTGAATTAGC+GGG 0.548872 8_1:+21478120 Msa1185010:three_prime_UTR
GAATGTGGTTCAGGACGGCT+TGG 0.549931 8_1:+21475644 Msa1185010:CDS
CTAGTGGGACGTGGCTATTG+TGG 0.559389 8_1:+21475614 Msa1185010:CDS
GCTTCTGCTTAAACTCAACT+AGG 0.581029 8_1:-21478360 None:intergenic
ATTTAGAAACAAGTTGAAGA+GGG 0.581599 8_1:+21475688 Msa1185010:CDS
TAAACAAAGAATGCCTCTAG+TGG 0.582286 8_1:+21475598 None:intergenic
TATTTAGAAACAAGTTGAAG+AGG 0.583872 8_1:+21475687 Msa1185010:CDS
AAGCGGTAGCAGTTGAGCAT+AGG 0.584941 8_1:+21478286 Msa1185010:three_prime_UTR
TATCACTCAACCTGAATTAG+CGG 0.592021 8_1:+21478119 Msa1185010:three_prime_UTR
CAATAGCCACGTCCCACTAG+AGG 0.623055 8_1:-21475611 None:intergenic
CTTGCAAAGCTGCAATCCAA+AGG 0.625966 8_1:-21478496 None:intergenic
CAGTGGAATGTGGTTCAGGA+CGG 0.631782 8_1:+21475639 Msa1185010:CDS
TATTGTGGCACAGTGGAATG+TGG 0.632981 8_1:+21475629 Msa1185010:CDS
AAACAAAGAATGCCTCTAGT+GGG 0.633215 8_1:+21475599 None:intergenic
AGAATGCCTCTAGTGGGACG+TGG 0.644063 8_1:+21475605 None:intergenic
TAGCAGTTGAGCATAGGCAT+AGG 0.647103 8_1:+21478292 Msa1185010:three_prime_UTR
AAGAAAAGTATATCTAAACA+TGG 0.669848 8_1:-21478403 None:intergenic
ACGTGGCTATTGTGGCACAG+TGG 0.762674 8_1:+21475622 Msa1185010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTGTATTAGAATTTTA+GGG - chr8_1:21476524-21476543 None:intergenic 10.0%
!!! TTAATTTGTATTAGAATTTT+AGG - chr8_1:21476525-21476544 None:intergenic 10.0%
!! ACAATTTATTTAAATGTTCT+GGG - chr8_1:21477181-21477200 None:intergenic 15.0%
!! AACAATTTATTTAAATGTTC+TGG - chr8_1:21477182-21477201 None:intergenic 15.0%
!!! TTTTTGTTACATTTTTATTG+AGG - chr8_1:21477216-21477235 None:intergenic 15.0%
!!! TATTTTTGCTGATTAATTAT+AGG + chr8_1:21477439-21477458 Msa1185010:intron 15.0%
!!! TGATTTTCTCAAATTATTAT+CGG + chr8_1:21477611-21477630 Msa1185010:intron 15.0%
!!! ATTGTTTTATTTCTTCCTTA+GGG - chr8_1:21476486-21476505 None:intergenic 20.0%
!!! TAATACCATTTTAGAATTTG+TGG + chr8_1:21476854-21476873 Msa1185010:intron 20.0%
!! CTATTAAATAATGCAGATAA+AGG - chr8_1:21477367-21477386 None:intergenic 20.0%
!! TATATTCAAATATGTTTGAC+TGG + chr8_1:21477407-21477426 Msa1185010:intron 20.0%
!!! CGTAAAAGTATTTATAGTTT+AGG + chr8_1:21477472-21477491 Msa1185010:intron 20.0%
!! ATATAACTCAGTGTAATTAT+AGG - chr8_1:21477592-21477611 None:intergenic 20.0%
!! TACTTAAACATAATTATGCA+CGG - chr8_1:21477769-21477788 None:intergenic 20.0%
!! AGTAATTAAGTTATGCATTA+TGG + chr8_1:21477785-21477804 Msa1185010:intron 20.0%
!!! TTTTGAATTTGATTTTGCAT+AGG + chr8_1:21477857-21477876 Msa1185010:intron 20.0%
!! AAACATTCAGATAAATGTTA+TGG + chr8_1:21478091-21478110 Msa1185010:three_prime_UTR 20.0%
!!! AAGAAAAGTATATCTAAACA+TGG - chr8_1:21478406-21478425 None:intergenic 20.0%
!! TATTTAGAAACAAGTTGAAG+AGG + chr8_1:21475687-21475706 Msa1185010:CDS 25.0%
!! ATTTAGAAACAAGTTGAAGA+GGG + chr8_1:21475688-21475707 Msa1185010:CDS 25.0%
! AAGCAACTCATAAAATTTAG+TGG + chr8_1:21475734-21475753 Msa1185010:intron 25.0%
! AACTCACTTTCATAATTGTA+TGG + chr8_1:21475810-21475829 Msa1185010:intron 25.0%
! TTCATAATTGTATGGAAGAA+AGG + chr8_1:21475818-21475837 Msa1185010:intron 25.0%
!!! TCTGGATTTTAAAGAATTGT+CGG - chr8_1:21475985-21476004 None:intergenic 25.0%
! ACACATAATGAAAGAAATTC+TGG - chr8_1:21476003-21476022 None:intergenic 25.0%
!!! TCTGAAACTGATATTTTTGA+AGG - chr8_1:21476130-21476149 None:intergenic 25.0%
!!! ATATTTGTTTTTCTGTCATG+AGG - chr8_1:21476446-21476465 None:intergenic 25.0%
!!! CATTGTTTTATTTCTTCCTT+AGG - chr8_1:21476487-21476506 None:intergenic 25.0%
!!! TTGTGTCATCTCATTTTTTT+TGG - chr8_1:21476638-21476657 None:intergenic 25.0%
! TAAACAAGTGTTTATTAGTG+GGG - chr8_1:21476729-21476748 None:intergenic 25.0%
! CTAAACAAGTGTTTATTAGT+GGG - chr8_1:21476730-21476749 None:intergenic 25.0%
! TCTAAACAAGTGTTTATTAG+TGG - chr8_1:21476731-21476750 None:intergenic 25.0%
!! TTCAACCACAAATTCTAAAA+TGG - chr8_1:21476862-21476881 None:intergenic 25.0%
!! AAACTTGTCCTAAAGATTTT+AGG - chr8_1:21477029-21477048 None:intergenic 25.0%
!! TGTTACATTTTTATTGAGGA+GGG - chr8_1:21477212-21477231 None:intergenic 25.0%
!! TTGTTACATTTTTATTGAGG+AGG - chr8_1:21477213-21477232 None:intergenic 25.0%
! AGATAAAGGCAAGAAAAAAT+GGG - chr8_1:21477353-21477372 None:intergenic 25.0%
! TAAATCACTCTTTAACTATG+AGG - chr8_1:21477683-21477702 None:intergenic 25.0%
! AGTGATTTATGACTCTAAAT+TGG + chr8_1:21477694-21477713 Msa1185010:intron 25.0%
! GTGATTTATGACTCTAAATT+GGG + chr8_1:21477695-21477714 Msa1185010:intron 25.0%
!!! CTAAATTGGGTTATTTTTTC+AGG + chr8_1:21477708-21477727 Msa1185010:intron 25.0%
!! ACTAAGAAGTATACTTTATG+AGG + chr8_1:21477811-21477830 Msa1185010:intron 25.0%
!! CTAAGAAGTATACTTTATGA+GGG + chr8_1:21477812-21477831 Msa1185010:intron 25.0%
!!! TTTAGTTTTGCTGCAAAATT+TGG + chr8_1:21477950-21477969 Msa1185010:intron 25.0%
! TTTCTATTACAGTCTGTTAA+AGG + chr8_1:21478461-21478480 Msa1185010:three_prime_UTR 25.0%
! AAGGAAAAAATAAAGACCTT+TGG + chr8_1:21478480-21478499 Msa1185010:three_prime_UTR 25.0%
! CAATCAATCGATTTCTATGA+TGG + chr8_1:21475766-21475785 Msa1185010:intron 30.0%
AATATCAGTTTCAGAGTCAA+AGG + chr8_1:21476135-21476154 Msa1185010:intron 30.0%
ATATCAGTTTCAGAGTCAAA+GGG + chr8_1:21476136-21476155 Msa1185010:intron 30.0%
TACCAAAAGACAACACTTAA+GGG - chr8_1:21476564-21476583 None:intergenic 30.0%
!!! TTTTTTTGGAACTCACAAAC+CGG - chr8_1:21476624-21476643 None:intergenic 30.0%
!!! CTCTCTTTTAGTTTAACTCT+CGG + chr8_1:21476658-21476677 Msa1185010:intron 30.0%
AAACAAGTGTTTATTAGTGG+GGG - chr8_1:21476728-21476747 None:intergenic 30.0%
CTAAAACAATCCTACAAGAT+CGG + chr8_1:21476883-21476902 Msa1185010:intron 30.0%
!! ACCTGAAAAGTGTTATAAGT+GGG - chr8_1:21476929-21476948 None:intergenic 30.0%
GCAACAATCCTAAAATCTTT+AGG + chr8_1:21477018-21477037 Msa1185010:intron 30.0%
TATCTTCATCCTCACTAAAT+AGG + chr8_1:21477308-21477327 Msa1185010:intron 30.0%
CAGATAAAGGCAAGAAAAAA+TGG - chr8_1:21477354-21477373 None:intergenic 30.0%
GAAAGAAAAACGAAAAACAC+CGG - chr8_1:21477905-21477924 None:intergenic 30.0%
!! TTTTCGTTTTTCTTTCTGAG+AGG + chr8_1:21477909-21477928 Msa1185010:intron 30.0%
! TCTTGCATCATTAGGAATTT+TGG + chr8_1:21478254-21478273 Msa1185010:three_prime_UTR 30.0%
!! AATTTTGGAGAATGTTGAAG+CGG + chr8_1:21478269-21478288 Msa1185010:three_prime_UTR 30.0%
! TCAATCGATTTCTATGATGG+AGG + chr8_1:21475769-21475788 Msa1185010:intron 35.0%
GGAAGTTTAACAAACACATG+TGG - chr8_1:21475848-21475867 None:intergenic 35.0%
AAGGCCAAAACAAATTCTTG+GGG - chr8_1:21475870-21475889 None:intergenic 35.0%
CAAGGCCAAAACAAATTCTT+GGG - chr8_1:21475871-21475890 None:intergenic 35.0%
AAGTAAAATCGTACACGTAC+GGG - chr8_1:21475894-21475913 None:intergenic 35.0%
CAAGTAAAATCGTACACGTA+CGG - chr8_1:21475895-21475914 None:intergenic 35.0%
AACATCTGTACCATGACAAT+CGG + chr8_1:21476087-21476106 Msa1185010:intron 35.0%
TAACCTATACCAATCTGATG+CGG + chr8_1:21476306-21476325 Msa1185010:intron 35.0%
TCTTGTCTTGTCAATCTCTT+GGG - chr8_1:21476384-21476403 None:intergenic 35.0%
ATCTTGTCTTGTCAATCTCT+TGG - chr8_1:21476385-21476404 None:intergenic 35.0%
ATAGTGTTAGCAAAACCCTA+AGG + chr8_1:21476468-21476487 Msa1185010:intron 35.0%
ACCAAAAGACAACACTTAAG+GGG - chr8_1:21476563-21476582 None:intergenic 35.0%
CTACCAAAAGACAACACTTA+AGG - chr8_1:21476565-21476584 None:intergenic 35.0%
!! AACTCACAAACCGGTTTTAT+GGG - chr8_1:21476615-21476634 None:intergenic 35.0%
TAGTTTAACTCTCGGATTTG+TGG + chr8_1:21476666-21476685 Msa1185010:intron 35.0%
CTCTACCATTGAACAATATC+AGG + chr8_1:21476751-21476770 Msa1185010:intron 35.0%
! CGATCTTGTAGGATTGTTTT+AGG - chr8_1:21476885-21476904 None:intergenic 35.0%
!! AAAAGTGTTATAAGTGGGAG+TGG - chr8_1:21476924-21476943 None:intergenic 35.0%
! TCCCACTTATAACACTTTTC+AGG + chr8_1:21476925-21476944 Msa1185010:intron 35.0%
!! GACCTGAAAAGTGTTATAAG+TGG - chr8_1:21476930-21476949 None:intergenic 35.0%
! AGGTCATGTCTTATTCGATA+CGG + chr8_1:21476945-21476964 Msa1185010:intron 35.0%
!! CTCTCTCTCGTGTTTTAAAT+AGG - chr8_1:21477056-21477075 None:intergenic 35.0%
TATTGATCAATCAACCTTCC+AGG + chr8_1:21477260-21477279 Msa1185010:intron 35.0%
ATTGATCAATCAACCTTCCA+GGG + chr8_1:21477261-21477280 Msa1185010:intron 35.0%
AGGATGAAGATAAAGACTTG+CGG - chr8_1:21477300-21477319 None:intergenic 35.0%
CAGAGAATACCTATTTAGTG+AGG - chr8_1:21477320-21477339 None:intergenic 35.0%
! TGCAAAATTTGGAGTCTAAC+AGG + chr8_1:21477961-21477980 Msa1185010:intron 35.0%
AAATTCAATGTTGCCCTTCA+CGG + chr8_1:21478036-21478055 Msa1185010:CDS 35.0%
TATCACTCAACCTGAATTAG+CGG + chr8_1:21478119-21478138 Msa1185010:three_prime_UTR 35.0%
! CTTTCCTCTCTTTTTCTTCA+AGG - chr8_1:21478160-21478179 None:intergenic 35.0%
ACGAGTGCGAGTTAAATAAA+AGG - chr8_1:21478220-21478239 None:intergenic 35.0%
TTGGATCTTCTTGCATCATT+AGG + chr8_1:21478246-21478265 Msa1185010:three_prime_UTR 35.0%
!! CTTCCCCCAAGAATTTGTTT+TGG + chr8_1:21475863-21475882 Msa1185010:intron 40.0%
AGGCCAAAACAAATTCTTGG+GGG - chr8_1:21475869-21475888 None:intergenic 40.0%
GCAAGGCCAAAACAAATTCT+TGG - chr8_1:21475872-21475891 None:intergenic 40.0%
! CGTACGTGTACGATTTTACT+TGG + chr8_1:21475893-21475912 Msa1185010:intron 40.0%
! GTACGATTTTACTTGGTCTG+CGG + chr8_1:21475900-21475919 Msa1185010:intron 40.0%
TCACCATGTGATTAAGTGGA+CGG - chr8_1:21475941-21475960 None:intergenic 40.0%
CTGATCACCATGTGATTAAG+TGG - chr8_1:21475945-21475964 None:intergenic 40.0%
CTTAATCACATGGTGATCAG+AGG + chr8_1:21475945-21475964 Msa1185010:intron 40.0%
TGTAGATTGTCGTGAAGTTG+AGG + chr8_1:21476047-21476066 Msa1185010:intron 40.0%
TACCATGACAATCGGCAATA+TGG + chr8_1:21476095-21476114 Msa1185010:intron 40.0%
! AACCATATTGCCGATTGTCA+TGG - chr8_1:21476100-21476119 None:intergenic 40.0%
ATGACAATCGGCAATATGGT+TGG + chr8_1:21476099-21476118 Msa1185010:intron 40.0%
AGAGCAATTGACGCAACAAT+TGG - chr8_1:21476180-21476199 None:intergenic 40.0%
GCATTGAATCGCGTTATCTA+GGG + chr8_1:21476235-21476254 Msa1185010:intron 40.0%
ATGACATCTTTGTAGCAGCA+TGG + chr8_1:21476268-21476287 Msa1185010:intron 40.0%
GGGTATTCATAGAATCAACG+AGG - chr8_1:21476364-21476383 None:intergenic 40.0%
CCAAAAGACAACACTTAAGG+GGG - chr8_1:21476562-21476581 None:intergenic 40.0%
!! ACTCACAAACCGGTTTTATG+GGG - chr8_1:21476614-21476633 None:intergenic 40.0%
!! GAACTCACAAACCGGTTTTA+TGG - chr8_1:21476616-21476635 None:intergenic 40.0%
CAAGCATGTTTGTGTGTGTT+AGG - chr8_1:21476696-21476715 None:intergenic 40.0%
CCAAGCCTGATATTGTTCAA+TGG - chr8_1:21476759-21476778 None:intergenic 40.0%
CCATTGAACAATATCAGGCT+TGG + chr8_1:21476756-21476775 Msa1185010:intron 40.0%
TGGTGCATGGATATAATGGT+AGG + chr8_1:21476776-21476795 Msa1185010:intron 40.0%
TGCATGGATATAATGGTAGG+TGG + chr8_1:21476779-21476798 Msa1185010:intron 40.0%
!! TGCTATCATATTTCTGGTGC+CGG - chr8_1:21476806-21476825 None:intergenic 40.0%
ACCAGAAATATGATAGCAGG+TGG + chr8_1:21476808-21476827 Msa1185010:intron 40.0%
ACCACCTGCTATCATATTTC+TGG - chr8_1:21476812-21476831 None:intergenic 40.0%
TATGATAGCAGGTGGTCTAA+TGG + chr8_1:21476816-21476835 Msa1185010:intron 40.0%
CGGGTTCAAACTTGGAATAT+TGG - chr8_1:21476987-21477006 None:intergenic 40.0%
ATTGTTGCATGTCTAAGCTC+GGG - chr8_1:21477006-21477025 None:intergenic 40.0%
GATTGTTGCATGTCTAAGCT+CGG - chr8_1:21477007-21477026 None:intergenic 40.0%
TTGATCAATCAACCTTCCAG+GGG + chr8_1:21477262-21477281 Msa1185010:intron 40.0%
TAAAGACTTGCGGTGCATAA+AGG - chr8_1:21477290-21477309 None:intergenic 40.0%
!! CTGATGTTTGCTATGAAGCA+CGG + chr8_1:21477516-21477535 Msa1185010:intron 40.0%
!! GTAATTATAGGCGTTGGTGT+TGG - chr8_1:21477580-21477599 None:intergenic 40.0%
CTCAGTGTAATTATAGGCGT+TGG - chr8_1:21477586-21477605 None:intergenic 40.0%
CACTCTTTAACTATGAGGCA+CGG - chr8_1:21477678-21477697 None:intergenic 40.0%
!! GAGTGACACTGACTATTTTC+CGG + chr8_1:21477883-21477902 Msa1185010:intron 40.0%
! AACAGGAAACCTTGTTGCTT+TGG + chr8_1:21477978-21477997 Msa1185010:intron 40.0%
AATTCAATGTTGCCCTTCAC+GGG + chr8_1:21478037-21478056 Msa1185010:CDS 40.0%
! GCTTTTCATTTTCCCGTGAA+GGG - chr8_1:21478052-21478071 None:intergenic 40.0%
ATCACTCAACCTGAATTAGC+GGG + chr8_1:21478120-21478139 Msa1185010:three_prime_UTR 40.0%
! TTTTCATGTCCCGCTAATTC+AGG - chr8_1:21478132-21478151 None:intergenic 40.0%
GAGACCTTGAAGAAAAAGAG+AGG + chr8_1:21478153-21478172 Msa1185010:three_prime_UTR 40.0%
GCTTCTGCTTAAACTCAACT+AGG - chr8_1:21478363-21478382 None:intergenic 40.0%
TATTGTGGCACAGTGGAATG+TGG + chr8_1:21475629-21475648 Msa1185010:CDS 45.0%
! TAAGTGGACGGACTCAAGAT+TGG - chr8_1:21475929-21475948 None:intergenic 45.0%
AGTCCGTCCACTTAATCACA+TGG + chr8_1:21475935-21475954 Msa1185010:intron 45.0%
ACTTCACGACAATCTACACG+CGG - chr8_1:21476045-21476064 None:intergenic 45.0%
GATTGTCGTGAAGTTGAGGT+TGG + chr8_1:21476051-21476070 Msa1185010:intron 45.0%
!! AGTCAAAGGGTGTCAACCAT+TGG + chr8_1:21476149-21476168 Msa1185010:intron 45.0%
GCAACAATTGGCTACTCCAA+TGG - chr8_1:21476168-21476187 None:intergenic 45.0%
CTCTACCTGCAAATGCAACT+AGG + chr8_1:21476196-21476215 Msa1185010:intron 45.0%
AATCGCCTAGTTGCATTTGC+AGG - chr8_1:21476204-21476223 None:intergenic 45.0%
AGATAACGCGATTCAATGCG+AGG - chr8_1:21476234-21476253 None:intergenic 45.0%
CGCATTGAATCGCGTTATCT+AGG + chr8_1:21476234-21476253 Msa1185010:intron 45.0%
ACACCGCATCAGATTGGTAT+AGG - chr8_1:21476312-21476331 None:intergenic 45.0%
TCACATACACCGCATCAGAT+TGG - chr8_1:21476318-21476337 None:intergenic 45.0%
ATCTGATGCGGTGTATGTGA+TGG + chr8_1:21476318-21476337 Msa1185010:intron 45.0%
!! CCCCCTTAAGTGTTGTCTTT+TGG + chr8_1:21476559-21476578 Msa1185010:intron 45.0%
TTTATGGGGTTGTGATGCAG+TGG - chr8_1:21476600-21476619 None:intergenic 45.0%
GCATCACAACCCCATAAAAC+CGG + chr8_1:21476602-21476621 Msa1185010:intron 45.0%
CACACACAAACATGCTTGTG+AGG + chr8_1:21476698-21476717 Msa1185010:intron 45.0%
!! ACAATATCAGGCTTGGTGCA+TGG + chr8_1:21476763-21476782 Msa1185010:intron 45.0%
!! GGCTTGGTGCATGGATATAA+TGG + chr8_1:21476772-21476791 Msa1185010:intron 45.0%
!! GCTATCATATTTCTGGTGCC+GGG - chr8_1:21476805-21476824 None:intergenic 45.0%
GGCACCAGAAATATGATAGC+AGG + chr8_1:21476805-21476824 Msa1185010:intron 45.0%
!! TCCTACAAGATCGGCTTTTG+AGG + chr8_1:21476892-21476911 Msa1185010:intron 45.0%
ACCTCAAAAGCCGATCTTGT+AGG - chr8_1:21476896-21476915 None:intergenic 45.0%
TCTAAGCTCGGGTTCAAACT+TGG - chr8_1:21476995-21477014 None:intergenic 45.0%
GAGAGAGAGAGAGATAGATC+AGG + chr8_1:21477099-21477118 Msa1185010:intron 45.0%
AGCTGGACACTATAGGATTC+AGG + chr8_1:21477123-21477142 Msa1185010:intron 45.0%
CCTGCAAATCCAAAGCAACA+AGG - chr8_1:21477990-21478009 None:intergenic 45.0%
!! CCTTGTTGCTTTGGATTTGC+AGG + chr8_1:21477987-21478006 Msa1185010:intron 45.0%
TTTGGATTTGCAGGAGCTGA+AGG + chr8_1:21477996-21478015 Msa1185010:intron 45.0%
! CGCTTTTCATTTTCCCGTGA+AGG - chr8_1:21478053-21478072 None:intergenic 45.0%
TAGCAGTTGAGCATAGGCAT+AGG + chr8_1:21478292-21478311 Msa1185010:three_prime_UTR 45.0%
CAGTGGAATGTGGTTCAGGA+CGG + chr8_1:21475639-21475658 Msa1185010:CDS 50.0%
AAATCGTACACGTACGGGCA+AGG - chr8_1:21475889-21475908 None:intergenic 50.0%
GGATATAATGGTAGGTGGCC+CGG + chr8_1:21476784-21476803 Msa1185010:intron 50.0%
AGAGAGATAGATCAGGCAGC+TGG + chr8_1:21477106-21477125 Msa1185010:intron 50.0%
ATCAGGCAGCTGGACACTAT+AGG + chr8_1:21477116-21477135 Msa1185010:intron 50.0%
TGCATAAAGGAACCCCTGGA+AGG - chr8_1:21477277-21477296 None:intergenic 50.0%
! GTGTCGACACGTCTAATCTC+AGG - chr8_1:21477547-21477566 None:intergenic 50.0%
!! CGTTGGTGTTGGACACTAAC+AGG - chr8_1:21477569-21477588 None:intergenic 50.0%
ATGAGGCACGGATTCAGACA+CGG - chr8_1:21477666-21477685 None:intergenic 50.0%
AACTCGCACTCGTTGCTCTT+TGG + chr8_1:21478227-21478246 Msa1185010:three_prime_UTR 50.0%
AAGCGGTAGCAGTTGAGCAT+AGG + chr8_1:21478286-21478305 Msa1185010:three_prime_UTR 50.0%
CAATAGCCACGTCCCACTAG+AGG - chr8_1:21475614-21475633 None:intergenic 55.0%
CTAGTGGGACGTGGCTATTG+TGG + chr8_1:21475614-21475633 Msa1185010:CDS 55.0%
ACGTGGCTATTGTGGCACAG+TGG + chr8_1:21475622-21475641 Msa1185010:CDS 55.0%
GGCACAGTGGAATGTGGTTC+AGG + chr8_1:21475635-21475654 Msa1185010:CDS 55.0%
GAATGTGGTTCAGGACGGCT+TGG + chr8_1:21475644-21475663 Msa1185010:CDS 55.0%
! TTCAGGACGGCTTGGAGTGT+TGG + chr8_1:21475652-21475671 Msa1185010:CDS 55.0%
! GTGGACGGACTCAAGATTGG+TGG - chr8_1:21475926-21475945 None:intergenic 55.0%
CTGCGTGTCAGTGTTGTGTC+TGG + chr8_1:21477637-21477656 Msa1185010:intron 55.0%
GCGGTGCATAAAGGAACCCC+TGG - chr8_1:21477281-21477300 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 21475608 21478508 21475608 ID=Msa1185010;Name=Msa1185010
chr8_1 mRNA 21475608 21478508 21475608 ID=Msa1185010-mRNA-1;Parent=Msa1185010;Name=Msa1185010-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0.5|0.5|1|1|2|425|64
chr8_1 exon 21475608 21475727 21475608 ID=Msa1185010-mRNA-1:exon:8111;Parent=Msa1185010-mRNA-1
chr8_1 exon 21478009 21478508 21478009 ID=Msa1185010-mRNA-1:exon:8112;Parent=Msa1185010-mRNA-1
chr8_1 CDS 21475608 21475727 21475608 ID=Msa1185010-mRNA-1:cds;Parent=Msa1185010-mRNA-1
chr8_1 CDS 21478009 21478083 21478009 ID=Msa1185010-mRNA-1:cds;Parent=Msa1185010-mRNA-1
chr8_1 three_prime_UTR 21478084 21478508 21478084 ID=Msa1185010-mRNA-1:three_prime_utr;Parent=Msa1185010-mRNA-1
Gene Sequence

>Msa1185010

ATGCCTCTAGTGGGACGTGGCTATTGTGGCACAGTGGAATGTGGTTCAGGACGGCTTGGAGTGTTGGTAGTCACTGATATATTTAGAAACAAGTTGAAGAGGGAACTCTGTAGTTTCAGTGAGCTGAAGGATTTTGAGAAGATGAAAAAATTCAATGTTGCCCTTCACGGGAAAATGAAAAGCGAGCATCACTGA
Protein sequence

>Msa1185010

MPLVGRGYCGTVECGSGRLGVLVVTDIFRNKLKRELCSFSELKDFEKMKKFNVALHGKMKSEHH*