AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1187080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 100 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCCAAGGATGCTGATTT+TGG 0.130542 8_1:+26402366 Msa1187080:five_prime_UTR
TGATGGTCATCAACAGAATT+TGG 0.184073 8_1:+26400846 Msa1187080:five_prime_UTR
TGATGGTCAACAACAGAATT+TGG 0.195932 8_1:+26402338 Msa1187080:five_prime_UTR
CGATGAGAATCTATTGATTC+TGG 0.202073 8_1:-26400439 None:intergenic
GCATATTTGGAATTGTTATT+TGG 0.208392 8_1:+26400705 Msa1187080:five_prime_UTR
AACTATCCCTATAGAAGATT+TGG 0.244456 8_1:-26402912 None:intergenic
CATAAGATACTAACTCTATT+TGG 0.262711 8_1:-26402251 None:intergenic
AGTTAGTATCTTATGTTTGA+TGG 0.280561 8_1:+26402259 Msa1187080:five_prime_UTR
TGAACTCTTGTTAAGAGAAT+TGG 0.288367 8_1:+26402641 Msa1187080:CDS
TTTGGTGTTCTTATGTTTGA+TGG 0.291886 8_1:+26400747 Msa1187080:five_prime_UTR
TTGTTGAGGGTTTGCATATT+TGG 0.292299 8_1:+26400692 Msa1187080:five_prime_UTR
AGGGATTAGTTGTTGGTTAG+AGG 0.295085 8_1:-26400925 None:intergenic
TCTTCCCCAAATCTTCTATA+GGG 0.306626 8_1:+26402906 Msa1187080:three_prime_UTR
TTCTTCCCCAAATCTTCTAT+AGG 0.308021 8_1:+26402905 Msa1187080:three_prime_UTR
CTTTGCAGAAACAATGATTA+AGG 0.317528 8_1:+26402730 Msa1187080:exon
CTCCATGCCGCTTGCTTCGC+TGG 0.321401 8_1:+26400946 Msa1187080:five_prime_UTR
GATGGTCATCAACAGAATTT+GGG 0.329683 8_1:+26400847 Msa1187080:five_prime_UTR
ATTCACATGGTCTTCATTGC+AGG 0.331338 8_1:+26400989 Msa1187080:five_prime_UTR
ACTATCCCTATAGAAGATTT+GGG 0.333353 8_1:-26402911 None:intergenic
GATGGTCAACAACAGAATTT+GGG 0.349213 8_1:+26402339 Msa1187080:five_prime_UTR
TATGATATTGTAAGTAACTT+TGG 0.357091 8_1:+26403008 Msa1187080:three_prime_UTR
ATTGTTTCTGCAAAGTGTAT+TGG 0.357614 8_1:-26402722 None:intergenic
AGCTGAGAGGTTCGAAATGA+TGG 0.370975 8_1:-26400332 None:intergenic
TTACCATGCACTCTTCTCAT+TGG 0.399767 8_1:+26402863 Msa1187080:three_prime_UTR
TTTCATATCAAACCTGTCAT+TGG 0.401205 8_1:-26403048 None:intergenic
TTGGAATTGTTATTTGGAAT+GGG 0.402943 8_1:+26400711 Msa1187080:five_prime_UTR
TGGTTAGAGGAAACTAATAT+TGG 0.404306 8_1:-26400912 None:intergenic
ATCTCACTAGACCTCATCCT+TGG 0.406098 8_1:-26400878 None:intergenic
TGAATGTATTTGGCTAAGAA+TGG 0.418759 8_1:+26402832 Msa1187080:three_prime_UTR
ATCTTCTATAGGGATAGTTT+AGG 0.422496 8_1:+26402916 Msa1187080:three_prime_UTR
TTTGGAATTGTTATTTGGAA+TGG 0.438457 8_1:+26400710 Msa1187080:five_prime_UTR
ATTCACATGGTCTTCATTGA+AGG 0.443441 8_1:+26402491 Msa1187080:CDS
CATCCCAAAATCAGCATCCT+TGG 0.443573 8_1:-26402370 None:intergenic
TGAGCGAATAATACCTCAAT+CGG 0.446299 8_1:-26400479 None:intergenic
GGCATGGAGGGATTAGTTGT+TGG 0.447957 8_1:-26400932 None:intergenic
GATGAGAATCTATTGATTCT+GGG 0.448633 8_1:-26400438 None:intergenic
AGTATGTCAAGGCTTCAAAT+TGG 0.456191 8_1:+26402784 Msa1187080:three_prime_UTR
CCATGCACTCTTCTCATTGG+TGG 0.456340 8_1:+26402866 Msa1187080:three_prime_UTR
GATTGAGATCTGCTTGTTGA+GGG 0.461078 8_1:+26400679 Msa1187080:five_prime_UTR
AAGCATAGTTATTGACATAG+AGG 0.464267 8_1:-26402956 None:intergenic
TAAGAGAATTGGTTAGAAGA+TGG 0.465771 8_1:+26402652 Msa1187080:CDS
AAGATTTGGGGAAGAAAGGA+AGG 0.479820 8_1:-26402898 None:intergenic
TGGTCAGAGGAAACTGATAT+TGG 0.481865 8_1:-26402417 None:intergenic
TCTCACTAGACCTCATCCTT+GGG 0.484127 8_1:-26400877 None:intergenic
ATAGAAGATTTGGGGAAGAA+AGG 0.497353 8_1:-26402902 None:intergenic
TAGAGGAAACTAATATTGGT+TGG 0.501315 8_1:-26400908 None:intergenic
TTTGATACTGTACCAATGAC+AGG 0.509351 8_1:+26403036 Msa1187080:three_prime_UTR
ATCTATTGATTCTGGGTCTG+TGG 0.520253 8_1:-26400431 None:intergenic
AAATCACAAAGATATGAGAT+TGG 0.521836 8_1:+26402677 Msa1187080:CDS
AGATAAACTCTCGAGAGAAA+AGG 0.523303 8_1:-26400208 None:intergenic
GGATTGAGATCTGCTTGTTG+AGG 0.527378 8_1:+26400678 Msa1187080:five_prime_UTR
TGCGGTGGTCGCAAGAGAGA+GGG 0.528744 8_1:-26400285 None:intergenic
CAGAGGAAACTGATATTGGT+TGG 0.530736 8_1:-26402413 None:intergenic
TACCTCAATCGGAAAACTAA+AGG 0.533584 8_1:-26400468 None:intergenic
ATTCTACCGGCGAAGTAGCA+TGG 0.534707 8_1:-26402453 None:intergenic
GAAGGATGGATCTCGAAATG+AGG 0.535143 8_1:-26400365 None:intergenic
AGCGAAGCAAGCGGCATGGA+GGG 0.543053 8_1:-26400944 None:intergenic
GGGGAATGAACCCAAGGATG+AGG 0.555484 8_1:+26400867 Msa1187080:five_prime_UTR
ATGCGGTGGTCGCAAGAGAG+AGG 0.556199 8_1:-26400286 None:intergenic
ACTCATCGCAAGCTGAAGGA+TGG 0.565811 8_1:-26400379 None:intergenic
GACTACAGCTGAAATTCTAC+CGG 0.568069 8_1:-26402466 None:intergenic
CAGCTGTAGTCTCATTCACA+TGG 0.568370 8_1:+26402478 Msa1187080:CDS
ATCCCTCCATGCTACTTCGC+CGG 0.573209 8_1:+26402447 Msa1187080:CDS
AACTAATATTGGTTGGAGCG+TGG 0.574255 8_1:-26400901 None:intergenic
ATGTCGAGTCGAGTATGTCA+AGG 0.574844 8_1:+26402773 Msa1187080:three_prime_UTR
AAGTAGCATGGAGGGATTAG+CGG 0.575147 8_1:-26402441 None:intergenic
TCTAGTGGTGGTGATGATGA+TGG 0.581366 8_1:+26402321 Msa1187080:five_prime_UTR
AGCATGGAGGGATTAGCGGC+TGG 0.584463 8_1:-26402437 None:intergenic
AAGAGTGCATGGTAAGACCT+TGG 0.591898 8_1:-26402855 None:intergenic
AGACACTCATCGCAAGCTGA+AGG 0.595339 8_1:-26400383 None:intergenic
AAGGATGGATCTCGAAATGA+GGG 0.596043 8_1:-26400364 None:intergenic
TACCAGCGAAGCAAGCGGCA+TGG 0.596697 8_1:-26400948 None:intergenic
CGGTGGTCGCAAGAGAGAGG+GGG 0.599107 8_1:-26400283 None:intergenic
AGTAGAGGAATTGTGTCTAG+TGG 0.600835 8_1:+26402306 Msa1187080:five_prime_UTR
GAATTTGGGGAATGAATCCA+AGG 0.600921 8_1:+26402353 Msa1187080:five_prime_UTR
ATTTGGGGAAGAAAGGAAGG+AGG 0.603147 8_1:-26402895 None:intergenic
CAGCGAAGCAAGCGGCATGG+AGG 0.605849 8_1:-26400945 None:intergenic
ATTTGATGAATCTGAACGAA+GGG 0.613459 8_1:-26400408 None:intergenic
TACCGGCGAAGTAGCATGGA+GGG 0.614416 8_1:-26402449 None:intergenic
AGGGATTAGCGGCTGGTCAG+AGG 0.617741 8_1:-26402430 None:intergenic
TATTTGGCTAAGAATGGCCA+AGG 0.618221 8_1:+26402838 Msa1187080:three_prime_UTR
GCGGTGGTCGCAAGAGAGAG+GGG 0.619645 8_1:-26400284 None:intergenic
GAATTTGGGGAATGAACCCA+AGG 0.632299 8_1:+26400861 Msa1187080:five_prime_UTR
AGGTGTCAAAATGTTCGTCA+AGG 0.633094 8_1:+26400791 Msa1187080:five_prime_UTR
TCAATTATATCTCATTCACA+TGG 0.636110 8_1:+26400976 Msa1187080:five_prime_UTR
AGTAGATGAACTATGTCTAG+TGG 0.637170 8_1:+26400814 Msa1187080:five_prime_UTR
CCACCAATGAGAAGAGTGCA+TGG 0.637746 8_1:-26402866 None:intergenic
AGAGGAATTGTGTCTAGTGG+TGG 0.650380 8_1:+26402309 Msa1187080:five_prime_UTR
GATGGATCTCGAAATGAGGG+AGG 0.651913 8_1:-26400361 None:intergenic
CTATCCCTATAGAAGATTTG+GGG 0.654217 8_1:-26402910 None:intergenic
AATTCTACCAGCGAAGCAAG+CGG 0.658478 8_1:-26400953 None:intergenic
TTCTAGCTGAAAGCATGCGG+TGG 0.666544 8_1:-26400300 None:intergenic
TCTAGTGGTGATGAAGATGA+TGG 0.668407 8_1:+26400829 Msa1187080:five_prime_UTR
ATGGATCTCGAAATGAGGGA+GGG 0.668738 8_1:-26400360 None:intergenic
AGGGAGGGTCGCGAGCTGAG+AGG 0.675163 8_1:-26400345 None:intergenic
GATTTGATGAATCTGAACGA+AGG 0.677141 8_1:-26400409 None:intergenic
AGATTCTAGCTGAAAGCATG+CGG 0.680718 8_1:-26400303 None:intergenic
ATGGTCAACAACAGAATTTG+GGG 0.683744 8_1:+26402340 Msa1187080:five_prime_UTR
ATGGTCATCAACAGAATTTG+GGG 0.685770 8_1:+26400848 Msa1187080:five_prime_UTR
CTACCGGCGAAGTAGCATGG+AGG 0.727997 8_1:-26402450 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGCTTTTTATTTTTATTT+TGG + chr8_1:26400635-26400654 Msa1187080:intron 10.0%
!!! ATAATTTAATGATTCAATTT+TGG + chr8_1:26401559-26401578 Msa1187080:intron 10.0%
!!! TAATTTAATGATTCAATTTT+GGG + chr8_1:26401560-26401579 Msa1187080:intron 10.0%
!!! TAATTTTAAAAATAGTTTGT+TGG + chr8_1:26401648-26401667 Msa1187080:intron 10.0%
!! CAATATTCTTAAAAATAATC+TGG - chr8_1:26400547-26400566 None:intergenic 15.0%
!!! AAGAATATTGTCATTTAATA+TGG + chr8_1:26400557-26400576 Msa1187080:intron 15.0%
!!! AGAATATTGTCATTTAATAT+GGG + chr8_1:26400558-26400577 Msa1187080:intron 15.0%
!! AATAATTATGTTATAGTTGA+TGG + chr8_1:26401705-26401724 Msa1187080:intron 15.0%
!! AAAATAAATTTGAATTAGCA+AGG - chr8_1:26402210-26402229 None:intergenic 15.0%
!!! GCTAATTCAAATTTATTTTT+AGG + chr8_1:26402211-26402230 Msa1187080:intron 15.0%
!!! AATTCAAATTTATTTTTAGG+TGG + chr8_1:26402214-26402233 Msa1187080:intron 15.0%
!!! TTTTGATATAAACAGTTTTA+AGG + chr8_1:26402283-26402302 Msa1187080:five_prime_UTR 15.0%
!!! ATCTTTATTTTGAATGTATT+TGG + chr8_1:26402822-26402841 Msa1187080:three_prime_UTR 15.0%
!!! TATTGTTGTTTAAATTGATC+AGG + chr8_1:26400603-26400622 Msa1187080:intron 20.0%
!!! ATTGTTGTTTAAATTGATCA+GGG + chr8_1:26400604-26400623 Msa1187080:intron 20.0%
!! ACATATGCAAAATAAAATCA+GGG - chr8_1:26401353-26401372 None:intergenic 20.0%
!! AACATATGCAAAATAAAATC+AGG - chr8_1:26401354-26401373 None:intergenic 20.0%
!!! TTTTTCCTTTATTCTAATTC+AGG + chr8_1:26401397-26401416 Msa1187080:intron 20.0%
!!! ATTTTCCGTTTTTTTATTGA+CGG + chr8_1:26401733-26401752 Msa1187080:intron 20.0%
!! TTATTCCGTCAATAAAAAAA+CGG - chr8_1:26401741-26401760 None:intergenic 20.0%
!! ATAAATCAGTAACTAATGAA+AGG - chr8_1:26401935-26401954 None:intergenic 20.0%
!!! TTACTTTTTGTTATCATGAA+AGG + chr8_1:26401965-26401984 Msa1187080:intron 20.0%
!! GCAATAAACAATATATGATA+TGG + chr8_1:26402049-26402068 Msa1187080:intron 20.0%
!!! GCATTGAATTATTGTATTTT+TGG + chr8_1:26402091-26402110 Msa1187080:intron 20.0%
!! GTTCATGATAACAAAAAAAT+TGG - chr8_1:26402188-26402207 None:intergenic 20.0%
!!! ATAGTTTTAAATTACAGTTG+CGG - chr8_1:26402551-26402570 None:intergenic 20.0%
!! AGACATGATTAAAACTAATA+TGG + chr8_1:26402574-26402593 Msa1187080:intron 20.0%
!! TATGATATTGTAAGTAACTT+TGG + chr8_1:26403008-26403027 Msa1187080:three_prime_UTR 20.0%
! TCTGGAAATAAAACATGAAA+AGG - chr8_1:26400529-26400548 None:intergenic 25.0%
!!! ATTTTGGTTTTGCTTTTGTT+AGG + chr8_1:26400651-26400670 Msa1187080:intron 25.0%
!!! TTTTGGTTTTGCTTTTGTTA+GGG + chr8_1:26400652-26400671 Msa1187080:intron 25.0%
!! GCATATTTGGAATTGTTATT+TGG + chr8_1:26400705-26400724 Msa1187080:five_prime_UTR 25.0%
!!! TTTGGAATTGTTATTTGGAA+TGG + chr8_1:26400710-26400729 Msa1187080:five_prime_UTR 25.0%
!!! TTGGAATTGTTATTTGGAAT+GGG + chr8_1:26400711-26400730 Msa1187080:five_prime_UTR 25.0%
!! TTTTGATATGAACAATGGTA+AGG + chr8_1:26400771-26400790 Msa1187080:five_prime_UTR 25.0%
! TCAATTATATCTCATTCACA+TGG + chr8_1:26400976-26400995 Msa1187080:five_prime_UTR 25.0%
!! GGTATCTTCTTTATTTGATA+TGG + chr8_1:26401010-26401029 Msa1187080:intron 25.0%
!! ATTTTCCTGCATATATTCAA+AGG + chr8_1:26401033-26401052 Msa1187080:intron 25.0%
!! TTTTCCTGCATATATTCAAA+GGG + chr8_1:26401034-26401053 Msa1187080:intron 25.0%
!! CTGTGATTGTTTAGTTATTT+TGG + chr8_1:26401095-26401114 Msa1187080:intron 25.0%
! TTGGTTGTTCATTATTAAGT+TGG + chr8_1:26401143-26401162 Msa1187080:intron 25.0%
! TGGTTGTTCATTATTAAGTT+GGG + chr8_1:26401144-26401163 Msa1187080:intron 25.0%
!!! CATGTTTTTTAGTTAAGCTA+TGG + chr8_1:26401180-26401199 Msa1187080:intron 25.0%
!!! TTTTATTGATTGAATGGTGA+AGG - chr8_1:26401313-26401332 None:intergenic 25.0%
!!! CATATGTTTTGAAAAGTGTT+AGG + chr8_1:26401366-26401385 Msa1187080:intron 25.0%
! CTCACAACTTTAATTAATCT+TGG + chr8_1:26401420-26401439 Msa1187080:intron 25.0%
! ATAATAAATGCACGAAATGA+AGG + chr8_1:26401457-26401476 Msa1187080:intron 25.0%
! ATGAAAATATTAGCCCAAAA+GGG - chr8_1:26401541-26401560 None:intergenic 25.0%
! TATGAAAATATTAGCCCAAA+AGG - chr8_1:26401542-26401561 None:intergenic 25.0%
!!! TTACTTTTTCATACGATTGT+TGG + chr8_1:26401778-26401797 Msa1187080:intron 25.0%
! CATATAAAAAAAACAAGCTC+AGG - chr8_1:26402150-26402169 None:intergenic 25.0%
! CATAAGATACTAACTCTATT+TGG - chr8_1:26402254-26402273 None:intergenic 25.0%
! AGTTAGTATCTTATGTTTGA+TGG + chr8_1:26402259-26402278 Msa1187080:five_prime_UTR 25.0%
!! GGTATCTTCTTTATTTGATA+TGG + chr8_1:26401010-26401029 Msa1187080:intron 25.0%
! AAATCACAAAGATATGAGAT+TGG + chr8_1:26402677-26402696 Msa1187080:CDS 25.0%
ATTTGATGAATCTGAACGAA+GGG - chr8_1:26400411-26400430 None:intergenic 30.0%
!! GATGAGAATCTATTGATTCT+GGG - chr8_1:26400441-26400460 None:intergenic 30.0%
! TGTCATTTAATATGGGTTTG+TGG + chr8_1:26400565-26400584 Msa1187080:intron 30.0%
!! TTTGGTGTTCTTATGTTTGA+TGG + chr8_1:26400747-26400766 Msa1187080:five_prime_UTR 30.0%
! ATGGCTTTTGATATGAACAA+TGG + chr8_1:26400766-26400785 Msa1187080:five_prime_UTR 30.0%
TAGAGGAAACTAATATTGGT+TGG - chr8_1:26400911-26400930 None:intergenic 30.0%
TGGTTAGAGGAAACTAATAT+TGG - chr8_1:26400915-26400934 None:intergenic 30.0%
TTTCCTGCATATATTCAAAG+GGG + chr8_1:26401035-26401054 Msa1187080:intron 30.0%
AATAAACACAACACAGTCAA+GGG - chr8_1:26401064-26401083 None:intergenic 30.0%
TTAAGTAGATCCATGAAGAT+TGG + chr8_1:26401118-26401137 Msa1187080:intron 30.0%
! GGTTGTTCATTATTAAGTTG+GGG + chr8_1:26401145-26401164 Msa1187080:intron 30.0%
!! TCATTATTAAGTTGGGGAAT+TGG + chr8_1:26401151-26401170 Msa1187080:intron 30.0%
! GGTAAGCTTAAATTTTCTCA+GGG - chr8_1:26401259-26401278 None:intergenic 30.0%
! TGGTAAGCTTAAATTTTCTC+AGG - chr8_1:26401260-26401279 None:intergenic 30.0%
!! GTAGGCTTTTATTGATTGAA+TGG - chr8_1:26401319-26401338 None:intergenic 30.0%
TGAAAATATTAGCCCAAAAG+GGG - chr8_1:26401540-26401559 None:intergenic 30.0%
TTGACGGAATAAAAGAAGAA+AGG + chr8_1:26401749-26401768 Msa1187080:intron 30.0%
! TTCCTTCTATAGCTTGATTT+TGG + chr8_1:26401885-26401904 Msa1187080:intron 30.0%
TTCCAAAATCAAGCTATAGA+AGG - chr8_1:26401890-26401909 None:intergenic 30.0%
!!! TTATTTTTAGGTGGAAGACT+TGG + chr8_1:26402223-26402242 Msa1187080:intron 30.0%
!!! TAAACAGTTTTAAGGAGTAG+AGG + chr8_1:26402291-26402310 Msa1187080:five_prime_UTR 30.0%
! TCTTTATTTGATATGGACCT+TGG + chr8_1:26402519-26402538 Msa1187080:intron 30.0%
AATATGGATTTCTTATCGCT+AGG + chr8_1:26402590-26402609 Msa1187080:intron 30.0%
TGAACTCTTGTTAAGAGAAT+TGG + chr8_1:26402641-26402660 Msa1187080:CDS 30.0%
TAAGAGAATTGGTTAGAAGA+TGG + chr8_1:26402652-26402671 Msa1187080:CDS 30.0%
!! ATTGTTTCTGCAAAGTGTAT+TGG - chr8_1:26402725-26402744 None:intergenic 30.0%
CTTTGCAGAAACAATGATTA+AGG + chr8_1:26402730-26402749 Msa1187080:exon 30.0%
TGAATGTATTTGGCTAAGAA+TGG + chr8_1:26402832-26402851 Msa1187080:three_prime_UTR 30.0%
ACTATCCCTATAGAAGATTT+GGG - chr8_1:26402914-26402933 None:intergenic 30.0%
AACTATCCCTATAGAAGATT+TGG - chr8_1:26402915-26402934 None:intergenic 30.0%
ATCTTCTATAGGGATAGTTT+AGG + chr8_1:26402916-26402935 Msa1187080:three_prime_UTR 30.0%
AAGCATAGTTATTGACATAG+AGG - chr8_1:26402959-26402978 None:intergenic 30.0%
TTTCATATCAAACCTGTCAT+TGG - chr8_1:26403051-26403070 None:intergenic 30.0%
AGATAAACTCTCGAGAGAAA+AGG - chr8_1:26400211-26400230 None:intergenic 35.0%
GATTTGATGAATCTGAACGA+AGG - chr8_1:26400412-26400431 None:intergenic 35.0%
!! CGATGAGAATCTATTGATTC+TGG - chr8_1:26400442-26400461 None:intergenic 35.0%
!!! TTCCTTTAGTTTTCCGATTG+AGG + chr8_1:26400466-26400485 Msa1187080:five_prime_UTR 35.0%
TACCTCAATCGGAAAACTAA+AGG - chr8_1:26400471-26400490 None:intergenic 35.0%
TGAGCGAATAATACCTCAAT+CGG - chr8_1:26400482-26400501 None:intergenic 35.0%
!! GTTTTGCTTTTGTTAGGGTT+TGG + chr8_1:26400657-26400676 Msa1187080:intron 35.0%
TTGTTGAGGGTTTGCATATT+TGG + chr8_1:26400692-26400711 Msa1187080:five_prime_UTR 35.0%
!! TATTTGGAATGGGTTTTTCG+CGG + chr8_1:26400721-26400740 Msa1187080:five_prime_UTR 35.0%
AGTAGATGAACTATGTCTAG+TGG + chr8_1:26400814-26400833 Msa1187080:five_prime_UTR 35.0%
TGATGGTCATCAACAGAATT+TGG + chr8_1:26400846-26400865 Msa1187080:five_prime_UTR 35.0%
GATGGTCATCAACAGAATTT+GGG + chr8_1:26400847-26400866 Msa1187080:five_prime_UTR 35.0%
ATGGTCATCAACAGAATTTG+GGG + chr8_1:26400848-26400867 Msa1187080:five_prime_UTR 35.0%
AAACCCCTTTGAATATATGC+AGG - chr8_1:26401041-26401060 None:intergenic 35.0%
CAATAAACACAACACAGTCA+AGG - chr8_1:26401065-26401084 None:intergenic 35.0%
ACTTGTAACATGTTGATCCA+TGG + chr8_1:26401220-26401239 Msa1187080:intron 35.0%
! ATCAGGGCATTAATAACAGT+AGG - chr8_1:26401337-26401356 None:intergenic 35.0%
GTGAGCCTGAATTAGAATAA+AGG - chr8_1:26401405-26401424 None:intergenic 35.0%
AGTTGTTCTCGTAACATTGT+AGG + chr8_1:26401499-26401518 Msa1187080:intron 35.0%
GAAAATATTAGCCCAAAAGG+GGG - chr8_1:26401539-26401558 None:intergenic 35.0%
! CAATTTTGGGCTAACATTGT+AGG + chr8_1:26401608-26401627 Msa1187080:intron 35.0%
TAGTGCCTCAAATTCCTTAT+TGG + chr8_1:26401861-26401880 Msa1187080:intron 35.0%
GGAAACCAATAAGGAATTTG+AGG - chr8_1:26401869-26401888 None:intergenic 35.0%
GCTATAGAAGGAAACCAATA+AGG - chr8_1:26401878-26401897 None:intergenic 35.0%
TTCTAAGTCAATGTCTAGGT+AGG + chr8_1:26401990-26402009 Msa1187080:intron 35.0%
TGATGTCAAGTACTACTAGT+AGG - chr8_1:26402029-26402048 None:intergenic 35.0%
AATGCCATCCAACAATAACA+GGG - chr8_1:26402076-26402095 None:intergenic 35.0%
!! TTTTTGGCACATTTGAACCT+TGG + chr8_1:26402107-26402126 Msa1187080:intron 35.0%
TGATGGTCAACAACAGAATT+TGG + chr8_1:26402338-26402357 Msa1187080:five_prime_UTR 35.0%
GATGGTCAACAACAGAATTT+GGG + chr8_1:26402339-26402358 Msa1187080:five_prime_UTR 35.0%
ATGGTCAACAACAGAATTTG+GGG + chr8_1:26402340-26402359 Msa1187080:five_prime_UTR 35.0%
!!! AATCCAAGGATGCTGATTTT+GGG + chr8_1:26402367-26402386 Msa1187080:five_prime_UTR 35.0%
ATTCACATGGTCTTCATTGA+AGG + chr8_1:26402491-26402510 Msa1187080:CDS 35.0%
! TCTTTCTTTTCTCGCAAAGA+TGG - chr8_1:26402621-26402640 None:intergenic 35.0%
AGTATGTCAAGGCTTCAAAT+TGG + chr8_1:26402784-26402803 Msa1187080:three_prime_UTR 35.0%
ATAGAAGATTTGGGGAAGAA+AGG - chr8_1:26402905-26402924 None:intergenic 35.0%
TTCTTCCCCAAATCTTCTAT+AGG + chr8_1:26402905-26402924 Msa1187080:three_prime_UTR 35.0%
TCTTCCCCAAATCTTCTATA+GGG + chr8_1:26402906-26402925 Msa1187080:three_prime_UTR 35.0%
CTATCCCTATAGAAGATTTG+GGG - chr8_1:26402913-26402932 None:intergenic 35.0%
TTTGATACTGTACCAATGAC+AGG + chr8_1:26403036-26403055 Msa1187080:three_prime_UTR 35.0%
! AGATTCTAGCTGAAAGCATG+CGG - chr8_1:26400306-26400325 None:intergenic 40.0%
AAGGATGGATCTCGAAATGA+GGG - chr8_1:26400367-26400386 None:intergenic 40.0%
!! ATCTATTGATTCTGGGTCTG+TGG - chr8_1:26400434-26400453 None:intergenic 40.0%
! GATTGAGATCTGCTTGTTGA+GGG + chr8_1:26400679-26400698 Msa1187080:five_prime_UTR 40.0%
AGGTGTCAAAATGTTCGTCA+AGG + chr8_1:26400791-26400810 Msa1187080:five_prime_UTR 40.0%
! TCTAGTGGTGATGAAGATGA+TGG + chr8_1:26400829-26400848 Msa1187080:five_prime_UTR 40.0%
! AACTAATATTGGTTGGAGCG+TGG - chr8_1:26400904-26400923 None:intergenic 40.0%
!! AGGGATTAGTTGTTGGTTAG+AGG - chr8_1:26400928-26400947 None:intergenic 40.0%
ATTCACATGGTCTTCATTGC+AGG + chr8_1:26400989-26401008 Msa1187080:five_prime_UTR 40.0%
AGATCCATGAAGATTGGTGT+TGG + chr8_1:26401124-26401143 Msa1187080:intron 40.0%
ACAACCAACACCAATCTTCA+TGG - chr8_1:26401131-26401150 None:intergenic 40.0%
GTTGATCCATGGTTACTACA+AGG + chr8_1:26401231-26401250 Msa1187080:intron 40.0%
TCGTAACATTGTAGGTCTTG+TGG + chr8_1:26401507-26401526 Msa1187080:intron 40.0%
! CGTAACATTGTAGGTCTTGT+GGG + chr8_1:26401508-26401527 Msa1187080:intron 40.0%
!! GTTTGTTGGCTTGAAATGCA+AGG + chr8_1:26401662-26401681 Msa1187080:intron 40.0%
! TGAGGCACTAATGTAGCTAT+GGG - chr8_1:26401851-26401870 None:intergenic 40.0%
! TTGAGGCACTAATGTAGCTA+TGG - chr8_1:26401852-26401871 None:intergenic 40.0%
GGACTTCTAAGTCAATGTCT+AGG + chr8_1:26401986-26402005 Msa1187080:intron 40.0%
GATATGGTCCCTGTTATTGT+TGG + chr8_1:26402065-26402084 Msa1187080:intron 40.0%
CAATGCCATCCAACAATAAC+AGG - chr8_1:26402077-26402096 None:intergenic 40.0%
CTCAGGAATATGAACAACAC+GGG - chr8_1:26402133-26402152 None:intergenic 40.0%
GCTCAGGAATATGAACAACA+CGG - chr8_1:26402134-26402153 None:intergenic 40.0%
AGTAGAGGAATTGTGTCTAG+TGG + chr8_1:26402306-26402325 Msa1187080:five_prime_UTR 40.0%
GAATTTGGGGAATGAATCCA+AGG + chr8_1:26402353-26402372 Msa1187080:five_prime_UTR 40.0%
!!! GAATCCAAGGATGCTGATTT+TGG + chr8_1:26402366-26402385 Msa1187080:five_prime_UTR 40.0%
CAGAGGAAACTGATATTGGT+TGG - chr8_1:26402416-26402435 None:intergenic 40.0%
TGGTCAGAGGAAACTGATAT+TGG - chr8_1:26402420-26402439 None:intergenic 40.0%
GACTACAGCTGAAATTCTAC+CGG - chr8_1:26402469-26402488 None:intergenic 40.0%
TTTGATATGGACCTTGGCAA+TGG + chr8_1:26402525-26402544 Msa1187080:intron 40.0%
TATTTGGCTAAGAATGGCCA+AGG + chr8_1:26402838-26402857 Msa1187080:three_prime_UTR 40.0%
TTACCATGCACTCTTCTCAT+TGG + chr8_1:26402863-26402882 Msa1187080:three_prime_UTR 40.0%
AAGATTTGGGGAAGAAAGGA+AGG - chr8_1:26402901-26402920 None:intergenic 40.0%
AGCTGAGAGGTTCGAAATGA+TGG - chr8_1:26400335-26400354 None:intergenic 45.0%
ATGGATCTCGAAATGAGGGA+GGG - chr8_1:26400363-26400382 None:intergenic 45.0%
GAAGGATGGATCTCGAAATG+AGG - chr8_1:26400368-26400387 None:intergenic 45.0%
! GGATTGAGATCTGCTTGTTG+AGG + chr8_1:26400678-26400697 Msa1187080:five_prime_UTR 45.0%
! ATGGGTTTTTCGCGGCAATT+TGG + chr8_1:26400729-26400748 Msa1187080:five_prime_UTR 45.0%
GAATTTGGGGAATGAACCCA+AGG + chr8_1:26400861-26400880 Msa1187080:five_prime_UTR 45.0%
TCTCACTAGACCTCATCCTT+GGG - chr8_1:26400880-26400899 None:intergenic 45.0%
ATCTCACTAGACCTCATCCT+TGG - chr8_1:26400881-26400900 None:intergenic 45.0%
AATTCTACCAGCGAAGCAAG+CGG - chr8_1:26400956-26400975 None:intergenic 45.0%
AGGGAACCTTGTAGTAACCA+TGG - chr8_1:26401240-26401259 None:intergenic 45.0%
AGGTCAACAAGGTGAACATG+TGG - chr8_1:26401280-26401299 None:intergenic 45.0%
GATTGTGCTGAAGGTCAACA+AGG - chr8_1:26401291-26401310 None:intergenic 45.0%
ATGGTGAAGGATTGTGCTGA+AGG - chr8_1:26401300-26401319 None:intergenic 45.0%
TGGTCCCTGTTATTGTTGGA+TGG + chr8_1:26402069-26402088 Msa1187080:intron 45.0%
AATATGAACAACACGGGCCA+AGG - chr8_1:26402127-26402146 None:intergenic 45.0%
AGAGGAATTGTGTCTAGTGG+TGG + chr8_1:26402309-26402328 Msa1187080:five_prime_UTR 45.0%
! TCTAGTGGTGGTGATGATGA+TGG + chr8_1:26402321-26402340 Msa1187080:five_prime_UTR 45.0%
CATCCCAAAATCAGCATCCT+TGG - chr8_1:26402373-26402392 None:intergenic 45.0%
!!! CAAGGATGCTGATTTTGGGA+TGG + chr8_1:26402371-26402390 Msa1187080:exon 45.0%
AAGTAGCATGGAGGGATTAG+CGG - chr8_1:26402444-26402463 None:intergenic 45.0%
CAGCTGTAGTCTCATTCACA+TGG + chr8_1:26402478-26402497 Msa1187080:CDS 45.0%
ATGTCGAGTCGAGTATGTCA+AGG + chr8_1:26402773-26402792 Msa1187080:three_prime_UTR 45.0%
! AAGAGTGCATGGTAAGACCT+TGG - chr8_1:26402858-26402877 None:intergenic 45.0%
ATTTGGGGAAGAAAGGAAGG+AGG - chr8_1:26402898-26402917 None:intergenic 45.0%
! TTCTAGCTGAAAGCATGCGG+TGG - chr8_1:26400303-26400322 None:intergenic 50.0%
GATGGATCTCGAAATGAGGG+AGG - chr8_1:26400364-26400383 None:intergenic 50.0%
ACTCATCGCAAGCTGAAGGA+TGG - chr8_1:26400382-26400401 None:intergenic 50.0%
AGACACTCATCGCAAGCTGA+AGG - chr8_1:26400386-26400405 None:intergenic 50.0%
!! GGCATGGAGGGATTAGTTGT+TGG - chr8_1:26400935-26400954 None:intergenic 50.0%
! TTGTGGGTTTCACCCCCTTT+TGG + chr8_1:26401524-26401543 Msa1187080:intron 50.0%
! TGTGGGTTTCACCCCCTTTT+GGG + chr8_1:26401525-26401544 Msa1187080:intron 50.0%
GGCACTAATGTAGCTATGGG+AGG - chr8_1:26401848-26401867 None:intergenic 50.0%
ATTCTACCGGCGAAGTAGCA+TGG - chr8_1:26402456-26402475 None:intergenic 50.0%
! CCACCAATGAGAAGAGTGCA+TGG - chr8_1:26402869-26402888 None:intergenic 50.0%
CCATGCACTCTTCTCATTGG+TGG + chr8_1:26402866-26402885 Msa1187080:three_prime_UTR 50.0%
GGGGAATGAACCCAAGGATG+AGG + chr8_1:26400867-26400886 Msa1187080:five_prime_UTR 55.0%
! ATCCCTCCATGCTACTTCGC+CGG + chr8_1:26402447-26402466 Msa1187080:CDS 55.0%
TACCGGCGAAGTAGCATGGA+GGG - chr8_1:26402452-26402471 None:intergenic 55.0%
TACAGTTGCGGCCATTGCCA+AGG - chr8_1:26402539-26402558 None:intergenic 55.0%
AGGGGGAGGGGAACTCGTTA+GGG - chr8_1:26400269-26400288 None:intergenic 60.0%
TGCGGTGGTCGCAAGAGAGA+GGG - chr8_1:26400288-26400307 None:intergenic 60.0%
ATGCGGTGGTCGCAAGAGAG+AGG - chr8_1:26400289-26400308 None:intergenic 60.0%
AGCGAAGCAAGCGGCATGGA+GGG - chr8_1:26400947-26400966 None:intergenic 60.0%
TACCAGCGAAGCAAGCGGCA+TGG - chr8_1:26400951-26400970 None:intergenic 60.0%
! AGGGATTAGCGGCTGGTCAG+AGG - chr8_1:26402433-26402452 None:intergenic 60.0%
! AGCATGGAGGGATTAGCGGC+TGG - chr8_1:26402440-26402459 None:intergenic 60.0%
CTACCGGCGAAGTAGCATGG+AGG - chr8_1:26402453-26402472 None:intergenic 60.0%
GAGGGGGAGGGGAACTCGTT+AGG - chr8_1:26400270-26400289 None:intergenic 65.0%
GTCGCAAGAGAGAGGGGGAG+GGG - chr8_1:26400281-26400300 None:intergenic 65.0%
GGTCGCAAGAGAGAGGGGGA+GGG - chr8_1:26400282-26400301 None:intergenic 65.0%
TGGTCGCAAGAGAGAGGGGG+AGG - chr8_1:26400283-26400302 None:intergenic 65.0%
CGGTGGTCGCAAGAGAGAGG+GGG - chr8_1:26400286-26400305 None:intergenic 65.0%
GCGGTGGTCGCAAGAGAGAG+GGG - chr8_1:26400287-26400306 None:intergenic 65.0%
CAGCGAAGCAAGCGGCATGG+AGG - chr8_1:26400948-26400967 None:intergenic 65.0%
! CTCCATGCCGCTTGCTTCGC+TGG + chr8_1:26400946-26400965 Msa1187080:five_prime_UTR 65.0%
AGGGAGGGTCGCGAGCTGAG+AGG - chr8_1:26400348-26400367 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 26400202 26403080 26400202 ID=Msa1187080;Name=Msa1187080
chr8_1 mRNA 26400202 26403080 26400202 ID=Msa1187080-mRNA-1;Parent=Msa1187080;Name=Msa1187080-mRNA-1;_AED=0.26;_eAED=0.26;_QI=781|1|1|1|0.33|0.25|4|334|85
chr8_1 exon 26400202 26400487 26400202 ID=Msa1187080-mRNA-1:exon:9316;Parent=Msa1187080-mRNA-1
chr8_1 exon 26400673 26401010 26400673 ID=Msa1187080-mRNA-1:exon:9317;Parent=Msa1187080-mRNA-1
chr8_1 exon 26402233 26402512 26402233 ID=Msa1187080-mRNA-1:exon:9318;Parent=Msa1187080-mRNA-1
chr8_1 exon 26402612 26403080 26402612 ID=Msa1187080-mRNA-1:exon:9319;Parent=Msa1187080-mRNA-1
chr8_1 five_prime_UTR 26400202 26400487 26400202 ID=Msa1187080-mRNA-1:five_prime_utr;Parent=Msa1187080-mRNA-1
chr8_1 five_prime_UTR 26400673 26401010 26400673 ID=Msa1187080-mRNA-1:five_prime_utr;Parent=Msa1187080-mRNA-1
chr8_1 five_prime_UTR 26402233 26402389 26402233 ID=Msa1187080-mRNA-1:five_prime_utr;Parent=Msa1187080-mRNA-1
chr8_1 CDS 26402390 26402512 26402390 ID=Msa1187080-mRNA-1:cds;Parent=Msa1187080-mRNA-1
chr8_1 CDS 26402612 26402746 26402612 ID=Msa1187080-mRNA-1:cds;Parent=Msa1187080-mRNA-1
chr8_1 three_prime_UTR 26402747 26403080 26402747 ID=Msa1187080-mRNA-1:three_prime_utr;Parent=Msa1187080-mRNA-1
Gene Sequence

>Msa1187080

ATGGTTCTGATGAGATTCACGCTCCAACCAATATCAGTTTCCTCTGACCAGCCGCTAATCCCTCCATGCTACTTCGCCGGTAGAATTTCAGCTGTAGTCTCATTCACATGGTCTTCATTGAAGGTTCTTCCATCTTTGCGAGAAAAGAAAGATGAACTCTTGTTAAGAGAATTGGTTAGAAGATGGTCAAATCACAAAGATATGAGATTGGAGAAAGTACAGAAAAATACGATCCAATACACTTTGCAGAAACAATGA
Protein sequence

>Msa1187080

MVLMRFTLQPISVSSDQPLIPPCYFAGRISAVVSFTWSSLKVLPSLREKKDELLLRELVRRWSNHKDMRLEKVQKNTIQYTLQKQ*