AlfalfaGEDB | Msa1187440

M. sativa cultivar zhongmu-4 / Msa1187440

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187440	XP_003628039.1	98.667	150	2	0	1	150	503	652	1.74e-100	311

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187440	sp\|O65468\|CRK8_ARATH	52.564	156	67	3	1	150	522	676	2.60e-44	157

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0374040	Msa1187440	0.802722	4.901799e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187440	MtrunA17_Chr8g0354351	98.667	150	2	0	1	150	503	652	1.60e-104	311
Msa1187440	MtrunA17_Chr8g0354391	76.159	151	35	1	1	150	104	254	3.27e-83	244
Msa1187440	MtrunA17_Chr8g0354281	80.000	150	29	1	1	150	505	653	2.63e-81	252
Msa1187440	MtrunA17_Chr8g0354331	74.000	150	39	0	1	150	270	419	9.00e-79	239
Msa1187440	MtrunA17_Chr8g0354421	77.333	150	34	0	1	150	533	682	1.27e-78	245
Msa1187440	MtrunA17_Chr8g0354441	74.667	150	38	0	1	150	520	669	1.84e-76	239
Msa1187440	MtrunA17_Chr8g0354431	75.333	150	37	0	1	150	512	661	4.78e-75	236
Msa1187440	MtrunA17_Chr3g0081001	67.333	150	49	0	1	150	513	662	1.06e-64	208
Msa1187440	MtrunA17_Chr3g0081011	66.667	150	50	0	1	150	509	658	6.14e-64	206
Msa1187440	MtrunA17_Chr3g0082941	63.333	150	46	1	1	150	343	483	1.99e-60	193

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187440	AT4G23160.1	52.564	156	67	3	1	150	528	682	2.27e-45	157
Msa1187440	AT4G23140.1	50.968	155	71	1	1	150	520	674	8.83e-45	155
Msa1187440	AT4G23140.2	50.968	155	71	1	1	150	526	680	9.39e-45	155
Msa1187440	AT4G23180.1	49.020	153	75	2	1	150	517	669	2.51e-44	154
Msa1187440	AT1G11410.2	51.572	159	63	4	1	150	577	730	4.72e-44	154
Msa1187440	AT1G11410.4	51.572	159	63	4	1	150	699	852	5.51e-44	154
Msa1187440	AT1G11410.1	51.572	159	63	4	1	150	692	845	5.77e-44	154
Msa1187440	AT1G11410.3	51.572	159	63	4	1	150	668	821	5.78e-44	154
Msa1187440	AT4G23150.1	51.799	139	61	1	1	133	505	643	7.84e-44	152
Msa1187440	AT1G65790.1	48.718	156	74	2	1	150	688	843	1.66e-43	152

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Find 34 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCCGATGTTTATAGCTT+TGG	0.359237	8_1:-27143584	Msa1187440:CDS
AACTCCAAAGCTATAAACAT+CGG	0.613550	8_1:+27143580	None:intergenic
AACTGAAAATGCTGGTGGCT+TGG	0.445077	8_1:+27142966	None:intergenic
AATCGGGCTATAAACTATCT+AGG	0.337963	8_1:+27142859	None:intergenic
AATGCTGGTGGCTTGGGATT+TGG	0.308851	8_1:+27142973	None:intergenic
AATTCTTTCTCTCAGACCAT+AGG	0.532039	8_1:-27143520	Msa1187440:CDS
ACAACAACGGTGGACATTGT+AGG	0.501919	8_1:+27143024	None:intergenic
ACAAGGGTTTCCGACGAAGA+AGG	0.551792	8_1:-27142943	Msa1187440:CDS
ACATGGAGACTTTGGTGTGA+AGG	0.581529	8_1:-27143159	Msa1187440:CDS
ACCCTTGTAACTGAAAATGC+TGG	0.590189	8_1:+27142958	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCCGATGTTTATAGCTT+TGG	-	chr8_1:27142912-27142931	Msa1187440:CDS	30.0%
AACTCCAAAGCTATAAACAT+CGG	+	chr8_1:27142919-27142938	None:intergenic	30.0%
ATATAAGGTTCATATCCAAG+AGG	+	chr8_1:27143151-27143170	None:intergenic	30.0%
ATGTATGATTCTTTGTGGAT+TGG	+	chr8_1:27143385-27143404	None:intergenic	30.0%
CTTCAATGTATGATTCTTTG+TGG	+	chr8_1:27143390-27143409	None:intergenic	30.0%
CACAAAGAATCATACATTGA+AGG	-	chr8_1:27143388-27143407	Msa1187440:intron	30.0%
GTAATGAAATGCATACACAT+AGG	-	chr8_1:27143415-27143434	Msa1187440:intron	30.0%
AAATCCGATGTTTATAGCTT+TGG	-	chr8_1:27142912-27142931	Msa1187440:CDS	30.0%
AACTCCAAAGCTATAAACAT+CGG	+	chr8_1:27142919-27142938	None:intergenic	30.0%
ATATAAGGTTCATATCCAAG+AGG	+	chr8_1:27143151-27143170	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	27142873	27143645	27142873	ID=Msa1187440;Name=Msa1187440
chr8_1	mRNA	27142873	27143645	27142873	ID=Msa1187440-mRNA-1;Parent=Msa1187440;Name=Msa1187440-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0\|0\|0\|1\|1\|1\|2\|0\|150
chr8_1	exon	27143502	27143645	27143502	ID=Msa1187440-mRNA-1:exon:9546;Parent=Msa1187440-mRNA-1
chr8_1	exon	27142873	27143181	27142873	ID=Msa1187440-mRNA-1:exon:9545;Parent=Msa1187440-mRNA-1
chr8_1	CDS	27143502	27143645	27143502	ID=Msa1187440-mRNA-1:cds;Parent=Msa1187440-mRNA-1
chr8_1	CDS	27142873	27143181	27142873	ID=Msa1187440-mRNA-1:cds;Parent=Msa1187440-mRNA-1

Gene Sequence

>Msa1187440

ATGGCTCCAGAATATGCTATGGTTGGACTGTTTTCAGTTAAATCCGATGTTTATAGCTTTGGAGTTCTTATATTAGAAATCATTTATGGAAAAAGAAATGGTGAATTCTTTCTCTCAGACCATAGGCAAAGTCTTCTATTACATACATGGAGACTTTGGTGTGAAGGAAAATGCTTGGAAAAAATCCATCCAATCCACAAAGAATCATACATTGAAGGTGAAGTAATGAAATGCATACACATAGGTTTATTGTGTGTACAAGAAGATGCAGCAGATCGACCTACAATGTCCACCGTTGTTGTCATGCTAGGTAGTGACACAATAACCCTTCCAAATCCCAAGCCACCAGCATTTTCAGTTACAAGGGTTTCCGACGAAGAAGGTACAACTTCAAAAAGTTCTAAGGATAATTATGTTAATGAAGTACCCATAACTATCGTCTCCCCTAGATAG

Protein sequence

>Msa1187440

MAPEYAMVGLFSVKSDVYSFGVLILEIIYGKRNGEFFLSDHRQSLLLHTWRLWCEGKCLEKIHPIHKESYIEGEVMKCIHIGLLCVQEDAADRPTMSTVVVMLGSDTITLPNPKPPAFSVTRVSDEEGTTSKSSKDNYVNEVPITIVSPR*