AlfalfaGEDB | Msa1195980

M. sativa cultivar zhongmu-4 / Msa1195980

KEGG: K04733|K13420
GO: -
PFAM: PF12799.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195980	XP_024629309.2	32.609	230	148	4	1	225	94	321	6.33e-18	100
Msa1195980	XP_024629309.2	34.158	202	130	2	18	218	138	337	1.64e-15	93.2
Msa1195980	XP_024629309.2	40.323	186	110	1	28	212	100	285	1.41e-22	115
Msa1195980	XP_024629309.2	84.783	598	62	1	1	569	393	990	0.0	1001

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195980	sp\|C0LGP4\|Y3475_ARATH	34.080	625	355	8	1	569	384	1007	1.99e-105	343
Msa1195980	sp\|C0LGP4\|Y3475_ARATH	31.858	226	152	2	6	230	94	318	3.13e-23	108
Msa1195980	sp\|C0LGP4\|Y3475_ARATH	32.973	185	123	1	31	214	71	255	1.25e-20	100
Msa1195980	sp\|C0LGP4\|Y3475_ARATH	29.339	242	140	3	1	211	209	450	2.71e-19	95.9
Msa1195980	sp\|C0LGP4\|Y3475_ARATH	31.606	193	131	1	20	211	330	522	3.28e-18	92.4
Msa1195980	sp\|C0LGP4\|Y3475_ARATH	32.035	231	125	3	12	211	124	353	2.16e-17	89.7
Msa1195980	sp\|C0LGP4\|Y3475_ARATH	36.111	144	92	0	68	211	60	203	1.16e-14	81.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195980	A0A396GNM3	32.609	230	148	4	1	225	94	321	3.02e-18	100
Msa1195980	A0A396GNM3	34.158	202	130	2	18	218	138	337	7.81e-16	93.2
Msa1195980	A0A396GNM3	40.323	186	110	1	28	212	100	285	6.72e-23	115
Msa1195980	A0A396GNM3	84.783	598	62	1	1	569	393	990	0.0	1001

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1107800	Msa1195980	0.807344	5.290771e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195980	MtrunA17_Chr8g0363111	84.783	598	62	1	1	569	393	990	0.0	1001
Msa1195980	MtrunA17_Chr8g0363111	40.323	186	110	1	28	212	100	285	1.29e-26	115
Msa1195980	MtrunA17_Chr8g0363111	32.609	230	148	4	1	225	94	321	5.82e-22	100
Msa1195980	MtrunA17_Chr8g0363111	34.158	202	130	2	18	218	138	337	1.50e-19	93.2
Msa1195980	MtrunA17_Chr8g0363241	76.087	598	114	1	1	569	151	748	0.0	906
Msa1195980	MtrunA17_Chr8g0363241	31.301	246	129	5	1	211	1	241	1.43e-19	92.8
Msa1195980	MtrunA17_Chr8g0363401	77.090	598	108	1	1	569	198	795	0.0	904
Msa1195980	MtrunA17_Chr8g0363401	27.753	227	129	5	45	241	45	266	3.75e-13	72.4
Msa1195980	MtrunA17_Chr8g0362961	76.589	598	111	1	1	569	633	1230	0.0	901
Msa1195980	MtrunA17_Chr8g0362961	33.023	215	140	4	1	212	316	529	3.52e-21	98.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195980	AT3G47570.1	34.080	625	355	8	1	569	384	1007	2.02e-106	343
Msa1195980	AT3G47570.1	31.858	226	152	2	6	230	94	318	3.18e-24	108
Msa1195980	AT3G47570.1	32.973	185	123	1	31	214	71	255	1.27e-21	100
Msa1195980	AT3G47570.1	29.339	242	140	3	1	211	209	450	2.75e-20	95.9
Msa1195980	AT3G47570.1	31.606	193	131	1	20	211	330	522	3.34e-19	92.4
Msa1195980	AT3G47570.1	32.035	231	125	3	12	211	124	353	2.20e-18	89.7
Msa1195980	AT3G47570.1	36.111	144	92	0	68	211	60	203	1.18e-15	81.3
Msa1195980	AT3G47090.1	30.658	623	377	7	1	569	385	1006	5.32e-95	312
Msa1195980	AT3G47090.1	32.110	218	141	2	1	211	258	475	3.23e-22	102
Msa1195980	AT3G47090.1	32.389	247	154	5	1	239	282	523	4.03e-21	98.6

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Find 106 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGTTGACACAACTAA+GGG	0.645428	8_1:-44404051	None:intergenic
AAAGCCTTCTAATGTCTTGT+TGG	0.406579	8_1:+44404588	Msa1195980:CDS
AAAGGAAATGTGTACAGCTA+TGG	0.471295	8_1:+44405389	Msa1195980:CDS
AAAGGAATTGTTTCTGTCAA+AGG	0.461692	8_1:+44405371	Msa1195980:CDS
AAATCTTGATTTCAAATCAT+TGG	0.325276	8_1:+44404401	Msa1195980:CDS
AAATTTAGTCCAACAAATTA+GGG	0.318645	8_1:+44405535	Msa1195980:CDS
AACTGCAATCATCTCACCAT+TGG	0.447215	8_1:-44404268	None:intergenic
AACTTGAAGACATGGATCAG+TGG	0.640025	8_1:+44405476	Msa1195980:CDS
AAGATCTCCATAATTGAATT+AGG	0.390630	8_1:-44405506	None:intergenic
AAGATTCTGTAACTCTTTGA+TGG	0.310197	8_1:-44403332	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATTCTGTAACTCTTTGA+TGG	-	chr8_1:44403335-44403354	None:intergenic	30.0%
TCTCTTAGAAAATTGAACGT+AGG	+	chr8_1:44403493-44403512	Msa1195980:CDS	30.0%
CAAAGTTACTAAGGATCTAA+TGG	-	chr8_1:44403695-44403714	None:intergenic	30.0%
AAAATGGTTGACACAACTAA+GGG	-	chr8_1:44404054-44404073	None:intergenic	30.0%
TCTTAACAAGATTTCGATGT+CGG	-	chr8_1:44404365-44404384	None:intergenic	30.0%
TGATTTCAAATCATTGGTGA+TGG	+	chr8_1:44404407-44404426	Msa1195980:intron	30.0%
TTATCCAACAAGACATTAGA+AGG	-	chr8_1:44404595-44404614	None:intergenic	30.0%
TGAATGTCATACACAAACTT+TGG	+	chr8_1:44404675-44404694	Msa1195980:CDS	30.0%
ATAAAAGAGGAGAAATAACC+TGG	-	chr8_1:44404721-44404740	None:intergenic	30.0%
AAATAACCTTCAAACTGAGT+GGG	-	chr8_1:44405081-44405100	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	44403271	44405897	44403271	ID=Msa1195980;Name=Msa1195980
chr8_1	mRNA	44403271	44405897	44403271	ID=Msa1195980-mRNA-1;Parent=Msa1195980;Name=Msa1195980-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|185\|569
chr8_1	exon	44403271	44404422	44403271	ID=Msa1195980-mRNA-1:exon:14348;Parent=Msa1195980-mRNA-1
chr8_1	exon	44404517	44404721	44404517	ID=Msa1195980-mRNA-1:exon:14349;Parent=Msa1195980-mRNA-1
chr8_1	exon	44405360	44405897	44405360	ID=Msa1195980-mRNA-1:exon:14350;Parent=Msa1195980-mRNA-1
chr8_1	CDS	44403271	44404422	44403271	ID=Msa1195980-mRNA-1:cds;Parent=Msa1195980-mRNA-1
chr8_1	CDS	44404517	44404721	44404517	ID=Msa1195980-mRNA-1:cds;Parent=Msa1195980-mRNA-1
chr8_1	CDS	44405360	44405712	44405360	ID=Msa1195980-mRNA-1:cds;Parent=Msa1195980-mRNA-1
chr8_1	three_prime_UTR	44405713	44405897	44405713	ID=Msa1195980-mRNA-1:three_prime_utr;Parent=Msa1195980-mRNA-1

Gene Sequence

>Msa1195980

ATGACCAACATGTCTTCTTTTTCTATAAATGATAATAATATAAATAGACCAATACCTAGTACCATCAAAGAGTTACAGAATCTTCAAGTGTTGGATCTTGGGAACAATCGACTACAAGGATCATTTATTGATGAGCTCTGTGAACTTCAAGAATTAGGTGAGTTGCATCTAGAAAATAATAAGCTTTCTGGTGTTTTACCAACATGTTTAGGAAATATGATTTCTCTTAGAAAATTGAACGTAGGATCTAACAATTTGAACTCTAAGATACCTTCCTCTTTTTGGAGTCTCAGAGATATCTTAGAGGTAGATTTGTCTTATAATGCTTTCAATGGGAATCTTCCACTTGAGATTGGGAATTTGAGAGCAATTATAGTTTTAGATCTATCAGGAAATAATATTTCAATAAACATTCCTTCAACCATTAGATCCTTAGTAACTTTGCAAAACCTATCATTAGCGCATAACAAACTGAATGGATCAATTCCATCATCACTTGGTGAAATGGTGAGCTTGACCTCCTTGGACTTGTCCCAAAATATGCTAACCGGTATTATTCCAAAATCTTTAGAATCACTTTCGTATCTTGAAAACATCAACTTCTCATATAACAGATTACAAGGAGAGATTCCTGATGGTGGGCCTTTCAAAAATTTCATGGCTGAATCTTTTATTCACAATGGAGCCCTTTGTGGAAATCCTCGCCTGCATATACCTCCGTGTGGGGAACAAGTTAAAAAATGGTCAATGGGAAAGAAGTTGTTATTCAAATGCATCAGTCCCTTAGTTGTGTCAACCATTTTGGTTGTTGCATGCATCATACTTTCAAAGCATAATAAAAGGAAAAAGATTCAAATTACTCTTGAAAGGGGTTTATCAACTTTAGGAGCTCTAAGAAGAATTTCCTATTATGAACTTGTGCAAGCAACTAATGGATTCAATGAGTGTAATTTACTTGGACAGGGGGGCTTTGGTTCTGTATATCGAGGGAACCTCCCCAATGGTGAGATGATTGCAGTTAAAGTAATTGATTTGCAGTCAGAGGCAAAAGTAAAGAGCTTTGATGTAGAATGCAATGCAATGAGAAATCTCCGACATCGAAATCTTGTTAAGATTATTTGTAGTTGTTCAAATCTTGATTTCAAATCATTGAATATAGATGTTGCATACGCATTGGAATATCTCCATCATGGTTCTTCAATACTCGTGGTTCATTGCGATCTAAAGCCTTCTAATGTCTTGTTGGATAAAAATATGGTTGCATATGTTAGTGATTTTGGTATTGCCAAGCTCATCGATGAAGGACAATCTGAATGTCATACACAAACTTTGGCTACTATTGGATACCTTGCACCAGAGTATGGATCTAAAGGAATTGTTTCTGTCAAAGGAAATGTGTACAGCTATGGGATTATGCTAATGGAAATCTTCACAAGAAGAAAGCCAATAGATGATATGTTTGTTGCAGAACTAAACTTGAAGACATGGATCAGTGGATCATTGCCTAATTCAATTATGGAGATCTTGGATTCAAATTTAGTCCAACAAATTAGGGAACAAATTGATGACATATTGACTCACATGTCATCTATTTTTGGTTTAGCCCTCAGTTGTTGTGAAGATTTACCTAAAGCAAGAATTAATATGGCAGATGTTATTACGTCGCTAATCAAAATCAAGACTTTGGTTCTTAGCACAAACAGGGTCTAG

Protein sequence

>Msa1195980

MTNMSSFSINDNNINRPIPSTIKELQNLQVLDLGNNRLQGSFIDELCELQELGELHLENNKLSGVLPTCLGNMISLRKLNVGSNNLNSKIPSSFWSLRDILEVDLSYNAFNGNLPLEIGNLRAIIVLDLSGNNISINIPSTIRSLVTLQNLSLAHNKLNGSIPSSLGEMVSLTSLDLSQNMLTGIIPKSLESLSYLENINFSYNRLQGEIPDGGPFKNFMAESFIHNGALCGNPRLHIPPCGEQVKKWSMGKKLLFKCISPLVVSTILVVACIILSKHNKRKKIQITLERGLSTLGALRRISYYELVQATNGFNECNLLGQGGFGSVYRGNLPNGEMIAVKVIDLQSEAKVKSFDVECNAMRNLRHRNLVKIICSCSNLDFKSLNIDVAYALEYLHHGSSILVVHCDLKPSNVLLDKNMVAYVSDFGIAKLIDEGQSECHTQTLATIGYLAPEYGSKGIVSVKGNVYSYGIMLMEIFTRRKPIDDMFVAELNLKTWISGSLPNSIMEILDSNLVQQIREQIDDILTHMSSIFGLALSCCEDLPKARINMADVITSLIKIKTLVLSTNRV*