AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1200600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1200600 MtrunA17_Chr8g0370111 96.569 204 7 0 1 204 370 573 4.81e-146 418
Msa1200600 MtrunA17_Chr8g0370111 37.241 145 89 1 4 148 258 400 3.60e-17 79.0
Msa1200600 MtrunA17_Chr5g0407461 79.710 207 37 3 1 204 381 585 7.69e-114 336
Msa1200600 MtrunA17_Chr5g0407461 37.324 142 87 1 4 145 292 431 9.52e-20 86.7
Msa1200600 MtrunA17_Chr5g0407461 33.803 142 92 1 4 145 269 408 2.97e-17 79.3
Msa1200600 MtrunA17_Chr4g0054721 67.262 168 55 0 1 168 321 488 2.99e-76 238
Msa1200600 MtrunA17_Chr4g0054721 31.081 148 100 1 1 148 206 351 7.95e-18 80.9
Msa1200600 MtrunA17_Chr4g0058541 41.718 163 94 1 1 162 284 446 1.26e-37 136
Msa1200600 MtrunA17_Chr4g0058541 36.434 129 79 2 23 150 214 340 2.47e-14 70.9
Msa1200600 MtrunA17_Chr5g0412701 41.718 163 94 1 1 162 231 393 5.29e-35 128
Msa1200600 MtrunA17_Chr3g0116991 38.693 199 94 4 1 171 258 456 6.16e-35 129
Msa1200600 MtrunA17_Chr4g0071581 35.897 195 120 2 1 194 108 298 7.62e-31 115
Msa1200600 MtrunA17_Chr2g0298891 37.912 182 107 2 1 181 124 300 4.64e-28 108
Msa1200600 MtrunA17_Chr7g0217081 39.041 146 78 6 3 141 174 315 1.08e-15 75.1
Msa1200600 MtrunA17_Chr7g0217081 37.063 143 85 5 3 141 222 363 3.26e-14 70.5
Msa1200600 MtrunA17_Chr8g0363851 34.375 128 83 1 4 131 644 770 1.39e-15 74.7
Msa1200600 MtrunA17_Chr8g0363851 33.562 146 95 2 1 145 547 691 1.19e-13 68.9
Msa1200600 MtrunA17_Chr8g0363851 30.435 138 92 3 1 136 617 752 1.33e-11 63.2
Msa1200600 MtrunA17_Chr7g0217061 35.664 143 87 5 3 141 141 282 1.72e-15 74.3
Msa1200600 MtrunA17_Chr7g0217061 30.252 119 79 4 26 141 117 234 8.73e-11 60.5
Msa1200600 MtrunA17_Chr7g0215671 35.461 141 87 4 4 141 121 260 4.30e-15 73.2
Msa1200600 MtrunA17_Chr7g0217101 31.928 166 108 5 21 182 3 167 4.72e-15 72.8
Msa1200600 MtrunA17_Chr3g0086371 32.877 146 91 4 1 143 79 220 5.80e-15 71.2
Msa1200600 MtrunA17_Chr3g0086371 28.378 148 104 1 3 150 58 203 7.40e-13 65.5
Msa1200600 MtrunA17_Chr3g0086371 28.676 136 95 1 13 148 45 178 6.82e-11 60.1
Msa1200600 MtrunA17_Chr7g0215631 35.664 143 85 6 4 141 113 253 8.20e-15 72.4
Msa1200600 MtrunA17_Chr7g0215631 34.677 124 76 5 3 122 160 282 2.46e-11 62.4
Msa1200600 MtrunA17_Chr8g0363891 33.824 136 88 2 4 138 179 313 9.56e-15 72.0
Msa1200600 MtrunA17_Chr8g0363891 31.206 141 95 2 1 140 223 362 1.84e-14 71.2
Msa1200600 MtrunA17_Chr3g0089961 35.345 116 73 2 20 134 564 678 1.95e-14 71.2
Msa1200600 MtrunA17_Chr8g0357151 33.133 166 84 5 3 141 379 544 4.12e-14 70.5
Msa1200600 MtrunA17_Chr8g0357151 36.806 144 86 5 3 142 235 377 7.94e-14 69.7
Msa1200600 MtrunA17_Chr8g0357151 34.899 149 90 6 3 145 211 358 4.47e-13 67.4
Msa1200600 MtrunA17_Chr8g0357151 34.722 144 89 5 3 142 163 305 4.77e-13 67.4
Msa1200600 MtrunA17_Chr8g0357151 33.784 148 91 6 4 145 140 286 7.81e-13 66.6
Msa1200600 MtrunA17_Chr8g0357151 31.469 143 93 5 3 141 451 592 6.42e-11 61.2
Msa1200600 MtrunA17_Chr8g0357151 32.886 149 93 6 3 145 187 334 6.99e-11 60.8
Msa1200600 MtrunA17_Chr3g0089991 35.965 114 71 2 24 136 569 681 1.12e-13 69.3
Msa1200600 MtrunA17_Chr3g0089421 32.374 139 88 3 11 148 554 687 2.11e-13 68.2
Msa1200600 MtrunA17_Chr8g0357171 32.164 171 87 6 3 144 176 346 2.43e-13 68.2
Msa1200600 MtrunA17_Chr8g0357171 32.168 143 92 5 3 141 248 389 3.77e-13 67.4
Msa1200600 MtrunA17_Chr3g0089601 34.109 129 79 3 21 148 560 683 3.01e-13 67.8
Msa1200600 MtrunA17_Chr5g0437331 33.562 146 87 8 7 144 343 486 3.42e-13 67.8
Msa1200600 MtrunA17_Chr3g0089591 30.657 137 93 2 1 136 593 728 3.78e-13 67.8
Msa1200600 MtrunA17_Chr3g0089591 33.051 118 77 2 18 134 563 679 6.67e-13 67.0
Msa1200600 MtrunA17_Chr3g0089501 35.043 117 74 2 21 136 560 675 4.66e-13 67.4
Msa1200600 MtrunA17_Chr8g0357191 34.965 143 88 5 4 142 128 269 4.81e-13 67.4
Msa1200600 MtrunA17_Chr8g0357191 32.215 149 94 6 3 145 271 418 1.68e-12 65.9
Msa1200600 MtrunA17_Chr8g0357191 33.566 143 90 5 3 141 367 508 1.41e-11 63.2
Msa1200600 MtrunA17_Chr8g0357191 31.469 143 93 5 3 141 319 460 2.27e-11 62.4
Msa1200600 MtrunA17_Chr8g0357191 33.333 144 91 5 3 142 151 293 2.35e-11 62.4
Msa1200600 MtrunA17_Chr2g0317831 32.192 146 94 4 20 162 437 580 1.64e-12 65.5
Msa1200600 MtrunA17_Chr2g0317831 33.077 130 85 1 14 143 48 175 1.65e-12 65.5
Msa1200600 MtrunA17_Chr7g0215061 33.099 142 90 5 4 141 125 265 3.51e-12 64.7
Msa1200600 MtrunA17_Chr7g0215061 31.757 148 92 6 3 141 172 319 3.27e-11 62.0
Msa1200600 MtrunA17_Chr3g0089911 34.862 109 69 2 15 122 555 662 5.09e-12 64.3
Msa1200600 MtrunA17_Chr3g0089211 32.308 130 82 3 20 148 608 732 5.96e-12 63.9
Msa1200600 MtrunA17_Chr3g0089251 33.051 118 77 2 20 136 590 706 7.86e-12 63.9
Msa1200600 MtrunA17_Chr7g0216261 31.138 167 87 5 3 142 15 180 8.13e-12 63.5
Msa1200600 MtrunA17_Chr3g0089651 32.558 129 81 3 21 148 566 689 9.98e-12 63.5
Msa1200600 MtrunA17_Chr7g0219371 34.043 141 87 5 3 138 268 407 1.08e-11 63.2
Msa1200600 MtrunA17_Chr3g0089471 31.298 131 88 2 7 136 596 725 1.47e-11 62.8
Msa1200600 MtrunA17_Chr3g0089761 31.538 130 83 3 20 148 561 685 1.83e-11 62.8
Msa1200600 MtrunA17_Chr3g0105881 29.054 148 100 2 1 145 189 334 1.99e-11 62.4
Msa1200600 MtrunA17_Chr3g0089781 32.203 118 78 2 20 136 561 677 2.25e-11 62.4
Msa1200600 MtrunA17_Chr2g0309721 32.540 126 79 3 24 148 562 682 2.67e-11 62.4
Msa1200600 MtrunA17_Chr7g0218991 30.282 142 73 4 27 142 127 268 3.35e-11 62.0
Msa1200600 MtrunA17_Chr4g0058861 33.103 145 91 6 3 142 690 833 3.36e-11 62.0
Msa1200600 MtrunA17_Chr8g0374561 37.984 129 76 4 13 138 648 775 4.88e-11 61.2
Msa1200600 MtrunA17_Chr6g0464211 32.298 161 102 6 3 157 467 626 7.14e-11 60.8
Msa1200600 MtrunA17_Chr2g0285811 32.414 145 71 5 3 144 152 272 8.47e-11 60.5
Msa1200600 MtrunA17_Chr7g0232451 30.667 150 97 6 3 146 205 353 8.67e-11 60.8
Msa1200600 MtrunA17_Chr3g0089811 32.787 122 79 3 17 136 557 677 8.85e-11 60.8
Msa1200600 MtrunA17_Chr8g0355271 36.923 130 73 7 27 149 123 250 9.52e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1200600 AT4G35470.1 71.782 202 55 2 1 202 350 549 1.30e-99 299
Msa1200600 AT4G35470.1 36.765 136 84 1 4 139 261 394 2.94e-19 85.5
Msa1200600 AT4G35470.1 32.192 146 97 1 3 148 283 426 6.46e-17 79.0
Msa1200600 AT4G35470.1 34.286 140 88 2 23 162 234 369 2.37e-12 65.5
Msa1200600 AT2G17440.1 69.948 193 57 1 1 193 334 525 4.30e-92 279
Msa1200600 AT2G17440.1 34.559 136 87 1 4 139 245 378 2.81e-15 73.9
Msa1200600 AT2G17440.1 37.008 127 78 1 22 148 217 341 1.15e-12 66.2
Msa1200600 AT4G35470.2 74.233 163 42 0 1 163 350 512 1.90e-81 252
Msa1200600 AT4G35470.2 36.765 136 84 1 4 139 261 394 3.24e-19 85.5
Msa1200600 AT4G35470.2 32.192 146 97 1 3 148 283 426 7.40e-17 78.6
Msa1200600 AT4G35470.2 34.286 140 88 2 23 162 234 369 1.69e-12 65.9
Msa1200600 AT3G11330.1 45.399 163 88 1 1 162 279 441 5.04e-40 142
Msa1200600 AT5G05850.1 44.172 163 90 1 1 162 285 447 3.63e-38 138
Msa1200600 AT3G26500.1 41.615 161 93 1 1 160 241 401 1.14e-36 133
Msa1200600 AT3G26500.1 35.965 114 67 2 24 133 172 283 2.76e-11 62.4
Msa1200600 AT4G26050.1 41.530 183 103 3 1 182 139 318 1.35e-33 124
Msa1200600 AT2G19330.1 39.344 183 107 2 1 182 142 321 4.53e-32 119
Msa1200600 AT1G12970.1 39.306 173 100 2 4 171 245 417 4.74e-32 120
Msa1200600 AT1G12970.1 37.692 130 78 2 22 150 171 298 2.77e-14 70.9
Msa1200600 AT4G29880.1 37.374 198 119 3 1 196 135 329 1.60e-27 107
Msa1200600 AT2G30105.1 33.784 148 93 2 1 145 165 310 6.04e-18 81.3
Msa1200600 AT3G15410.1 35.156 128 81 1 14 141 47 172 4.26e-15 73.6
Msa1200600 AT3G15410.1 27.500 160 111 3 1 159 80 235 1.34e-14 72.0
Msa1200600 AT3G15410.1 28.042 189 107 5 1 162 393 579 2.94e-14 71.2
Msa1200600 AT3G15410.1 30.882 136 85 3 17 148 73 203 1.34e-11 63.2
Msa1200600 AT3G15410.2 35.156 128 81 1 14 141 53 178 4.61e-15 73.6
Msa1200600 AT3G15410.2 27.500 160 111 3 1 159 86 241 1.40e-14 72.0
Msa1200600 AT3G15410.2 28.042 189 107 5 1 162 399 585 2.56e-14 71.2
Msa1200600 AT3G15410.2 30.882 136 85 3 17 148 79 209 1.49e-11 63.2
Msa1200600 AT1G27180.3 31.690 142 95 2 1 141 906 1046 3.91e-13 67.8
Msa1200600 AT1G27180.3 30.147 136 91 3 1 134 812 945 3.23e-11 62.4
Msa1200600 AT1G27180.5 31.690 142 95 2 1 141 904 1044 4.47e-13 67.8
Msa1200600 AT1G27180.5 29.630 135 93 2 1 134 810 943 3.48e-11 62.4
Msa1200600 AT1G27180.8 31.690 142 95 2 1 141 904 1044 4.47e-13 67.8
Msa1200600 AT1G27180.8 29.630 135 93 2 1 134 810 943 3.48e-11 62.4
Msa1200600 AT1G27180.2 31.690 142 95 2 1 141 906 1046 4.52e-13 67.8
Msa1200600 AT1G27180.2 30.147 136 91 3 1 134 812 945 3.45e-11 62.4
Msa1200600 AT1G27180.1 31.690 142 95 2 1 141 1068 1208 4.70e-13 67.8
Msa1200600 AT1G27180.1 29.630 135 93 2 1 134 974 1107 3.80e-11 62.0
Msa1200600 AT1G27180.7 31.690 142 95 2 1 141 730 870 4.94e-13 67.8
Msa1200600 AT1G27180.7 29.630 135 93 2 1 134 636 769 3.88e-11 62.0
Msa1200600 AT1G27180.6 31.690 142 95 2 1 141 730 870 4.94e-13 67.8
Msa1200600 AT1G27180.6 29.630 135 93 2 1 134 636 769 3.88e-11 62.0
Msa1200600 AT1G27180.4 31.690 142 95 2 1 141 730 870 4.94e-13 67.8
Msa1200600 AT1G27180.4 29.630 135 93 2 1 134 636 769 3.88e-11 62.0
Msa1200600 AT2G26330.1 32.394 142 91 5 4 141 371 511 4.49e-12 64.7
Msa1200600 AT3G11080.2 34.028 144 88 6 3 141 140 281 1.50e-11 63.2
Msa1200600 AT3G11080.1 34.028 144 88 6 3 141 140 281 1.50e-11 63.2
Msa1200600 AT5G07910.1 33.113 151 98 2 1 150 80 228 1.55e-11 62.4
Msa1200600 AT5G07910.1 26.207 145 105 1 1 145 57 199 4.50e-11 60.8

Find 40 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCGGCACAAAGCTCTTTA+AGG 0.151289 8_1:-53339830 None:intergenic
GTAGCAAAACACAAACTTTC+AGG 0.205801 8_1:-53340011 None:intergenic
CCTTGAAATGCTTGAGGAAT+TGG 0.240452 8_1:+53340103 Msa1200600:CDS
TTAATTTGTCCTACTGCTTC+AGG 0.268479 8_1:-53339873 None:intergenic
GTCGAATTTGATTAACCTTA+AGG 0.303726 8_1:+53339953 Msa1200600:CDS
TGGTGGGGTTGATTATATTA+AGG 0.311821 8_1:+53341019 Msa1200600:CDS
AGATCATTGCCAAGGTCTAT+TGG 0.337149 8_1:+53340077 Msa1200600:CDS
AACTCACATCAAGTTCCTTA+AGG 0.344086 8_1:-53339968 None:intergenic
AGTGAGCATTCTAAAAGAAT+AGG 0.380907 8_1:-53340156 None:intergenic
AGCTTTGAGCCGATTGTAAT+CGG 0.384731 8_1:-53339847 None:intergenic
CCAATTCCTCAAGCATTTCA+AGG 0.392572 8_1:-53340103 None:intergenic
AAGATTGTTAAGATGAATGT+TGG 0.398024 8_1:+53340035 Msa1200600:CDS
TCCAAGACATGTAGTTGAAA+AGG 0.408070 8_1:+53340223 Msa1200600:CDS
TGGCTGAGCTTGTAGAGAAA+GGG 0.420363 8_1:+53340910 Msa1200600:CDS
GATCATTGCCAAGGTCTATT+GGG 0.425365 8_1:+53340078 Msa1200600:CDS
TTAAATCACAGCCACTAATC+AGG 0.434821 8_1:+53340937 Msa1200600:CDS
GTGAGCATTCTAAAAGAATA+GGG 0.444295 8_1:-53340155 None:intergenic
AGCTTTGTGCCGATTACAAT+CGG 0.464135 8_1:+53339838 Msa1200600:CDS
ATCGGCACAAAGCTCTTTAA+GGG 0.466184 8_1:-53339829 None:intergenic
CCAAGACATGTAGTTGAAAA+GGG 0.496282 8_1:+53340224 Msa1200600:CDS
GAAGAAATTCCACACTCAAT+CGG 0.496855 8_1:+53339795 Msa1200600:CDS
ATGGCTGAGCTTGTAGAGAA+AGG 0.501550 8_1:+53340909 Msa1200600:CDS
AGATTGTTAAGATGAATGTT+GGG 0.528167 8_1:+53340036 Msa1200600:CDS
TCAAGGTTCCCAATAGACCT+TGG 0.544491 8_1:-53340086 None:intergenic
TGGGAACCTTGAAATGCTTG+AGG 0.571340 8_1:+53340097 Msa1200600:CDS
TGTAGTTGAAAAGGGAGCAC+AGG 0.577585 8_1:+53340232 Msa1200600:CDS
CTAACACAAGAAAGCGTGGT+GGG 0.581619 8_1:+53341003 Msa1200600:CDS
ATAGGCAGTTGTGCAGTACA+TGG 0.583186 8_1:+53340890 Msa1200600:intron
TGTCTTGGAGGAACTTCAAG+AGG 0.587769 8_1:-53340209 None:intergenic
GGCTGAGCTTGTAGAGAAAG+GGG 0.595937 8_1:+53340911 Msa1200600:CDS
ATCAAATTCGACATTGTCGT+AGG 0.597782 8_1:-53339942 None:intergenic
CTGATATGAGATCATTGCCA+AGG 0.599896 8_1:+53340069 Msa1200600:CDS
CTACAATGACCGATTGAGTG+TGG 0.621676 8_1:-53339804 None:intergenic
AAAGCTTTGCCTGAAGCAGT+AGG 0.623092 8_1:+53339864 Msa1200600:CDS
TAACACAAGAAAGCGTGGTG+GGG 0.645448 8_1:+53341004 Msa1200600:CDS
ACCTCACAGATAGAATCTCA+AGG 0.658969 8_1:-53339899 None:intergenic
TAGACTTCGAGTATTGAGAG+TGG 0.666026 8_1:+53340178 Msa1200600:CDS
AAGTCTAACACAAGAAAGCG+TGG 0.674581 8_1:+53340999 Msa1200600:CDS
GCCTTGAGATTCTATCTGTG+AGG 0.675179 8_1:+53339898 Msa1200600:CDS
TCTAACACAAGAAAGCGTGG+TGG 0.731457 8_1:+53341002 Msa1200600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTAAATT+TGG + chr8_1:53340325-53340344 Msa1200600:intron 0.0%
!!! TTTTTTTTTTTTTTTAAATT+TGG + chr8_1:53340325-53340344 Msa1200600:intron 0.0%
!!! AAATTTTTAAAAAAGTACAA+AGG + chr8_1:53340508-53340527 Msa1200600:intron 10.0%
!!! CTTTATATTTATTTTTTTTG+TGG - chr8_1:53340676-53340695 None:intergenic 10.0%
!!! AAATTTTTAAAAAAGTACAA+AGG + chr8_1:53340508-53340527 Msa1200600:intron 10.0%
!!! CTTTATATTTATTTTTTTTG+TGG - chr8_1:53340676-53340695 None:intergenic 10.0%
!! AAAAAACGTAAAGTTAATAA+GGG - chr8_1:53340298-53340317 None:intergenic 15.0%
!! AAAAAAACGTAAAGTTAATA+AGG - chr8_1:53340299-53340318 None:intergenic 15.0%
!!! TTGTAAAACTATTTTACAAA+AGG - chr8_1:53340462-53340481 None:intergenic 15.0%
!! TACTATATAAAGATCAAATT+TGG + chr8_1:53340602-53340621 Msa1200600:intron 15.0%
!!! TATATTTATTTTTTTTGTGG+TGG - chr8_1:53340673-53340692 None:intergenic 15.0%
!! CTAAATTTAACCAAAATATT+TGG - chr8_1:53340759-53340778 None:intergenic 15.0%
!! AAAAAACGTAAAGTTAATAA+GGG - chr8_1:53340298-53340317 None:intergenic 15.0%
!! AAAAAAACGTAAAGTTAATA+AGG - chr8_1:53340299-53340318 None:intergenic 15.0%
!!! TTGTAAAACTATTTTACAAA+AGG - chr8_1:53340462-53340481 None:intergenic 15.0%
!! TACTATATAAAGATCAAATT+TGG + chr8_1:53340602-53340621 Msa1200600:intron 15.0%
!!! TATATTTATTTTTTTTGTGG+TGG - chr8_1:53340673-53340692 None:intergenic 15.0%
!! CTAAATTTAACCAAAATATT+TGG - chr8_1:53340759-53340778 None:intergenic 15.0%
!!! TTACAAAAGGCTTTTTTATT+TGG - chr8_1:53340449-53340468 None:intergenic 20.0%
!! TATAAAGATCAAATTTGGTA+AGG + chr8_1:53340607-53340626 Msa1200600:intron 20.0%
!! ATTTAACCAAAATATTTGGT+GGG - chr8_1:53340755-53340774 None:intergenic 20.0%
!! AATTTAACCAAAATATTTGG+TGG - chr8_1:53340756-53340775 None:intergenic 20.0%
!!! TTACAAAAGGCTTTTTTATT+TGG - chr8_1:53340449-53340468 None:intergenic 20.0%
!! TATAAAGATCAAATTTGGTA+AGG + chr8_1:53340607-53340626 Msa1200600:intron 20.0%
!! ATTTAACCAAAATATTTGGT+GGG - chr8_1:53340755-53340774 None:intergenic 20.0%
!! AATTTAACCAAAATATTTGG+TGG - chr8_1:53340756-53340775 None:intergenic 20.0%
! AAGATTGTTAAGATGAATGT+TGG + chr8_1:53340035-53340054 Msa1200600:CDS 25.0%
! AGATTGTTAAGATGAATGTT+GGG + chr8_1:53340036-53340055 Msa1200600:CDS 25.0%
! AATCACATATTTAAGAGACA+CGG + chr8_1:53340707-53340726 Msa1200600:intron 25.0%
! AAGATTGTTAAGATGAATGT+TGG + chr8_1:53340035-53340054 Msa1200600:CDS 25.0%
! AGATTGTTAAGATGAATGTT+GGG + chr8_1:53340036-53340055 Msa1200600:CDS 25.0%
! AATCACATATTTAAGAGACA+CGG + chr8_1:53340707-53340726 Msa1200600:intron 25.0%
GTCGAATTTGATTAACCTTA+AGG + chr8_1:53339953-53339972 Msa1200600:CDS 30.0%
! GTGAGCATTCTAAAAGAATA+GGG - chr8_1:53340158-53340177 None:intergenic 30.0%
! AGTGAGCATTCTAAAAGAAT+AGG - chr8_1:53340159-53340178 None:intergenic 30.0%
AAAGTACAAAGGACTCTTTA+AGG + chr8_1:53340519-53340538 Msa1200600:intron 30.0%
!!! TTTATTTTTTTTGTGGTGGC+CGG - chr8_1:53340669-53340688 None:intergenic 30.0%
TTCACTATTTCATTACGTGT+TGG + chr8_1:53340859-53340878 Msa1200600:intron 30.0%
GTCGAATTTGATTAACCTTA+AGG + chr8_1:53339953-53339972 Msa1200600:CDS 30.0%
! GTGAGCATTCTAAAAGAATA+GGG - chr8_1:53340158-53340177 None:intergenic 30.0%
! AGTGAGCATTCTAAAAGAAT+AGG - chr8_1:53340159-53340178 None:intergenic 30.0%
AAAGTACAAAGGACTCTTTA+AGG + chr8_1:53340519-53340538 Msa1200600:intron 30.0%
!!! TTTATTTTTTTTGTGGTGGC+CGG - chr8_1:53340669-53340688 None:intergenic 30.0%
TTCACTATTTCATTACGTGT+TGG + chr8_1:53340859-53340878 Msa1200600:intron 30.0%
GAAGAAATTCCACACTCAAT+CGG + chr8_1:53339795-53339814 Msa1200600:CDS 35.0%
!! TTAATTTGTCCTACTGCTTC+AGG - chr8_1:53339876-53339895 None:intergenic 35.0%
ATCAAATTCGACATTGTCGT+AGG - chr8_1:53339945-53339964 None:intergenic 35.0%
AACTCACATCAAGTTCCTTA+AGG - chr8_1:53339971-53339990 None:intergenic 35.0%
!! TGATGTGAGTTTTAATGAGC+TGG + chr8_1:53339980-53339999 Msa1200600:CDS 35.0%
GTAGCAAAACACAAACTTTC+AGG - chr8_1:53340014-53340033 None:intergenic 35.0%
! TTTTCAACTACATGTCTTGG+AGG - chr8_1:53340224-53340243 None:intergenic 35.0%
TCCAAGACATGTAGTTGAAA+AGG + chr8_1:53340223-53340242 Msa1200600:CDS 35.0%
CCAAGACATGTAGTTGAAAA+GGG + chr8_1:53340224-53340243 Msa1200600:CDS 35.0%
TGCATAATATATGCAAGGTC+CGG + chr8_1:53340633-53340652 Msa1200600:intron 35.0%
!!! TTATTTTTTTTGTGGTGGCC+GGG - chr8_1:53340668-53340687 None:intergenic 35.0%
ATTTAAGAGACACGGCTTAT+CGG + chr8_1:53340715-53340734 Msa1200600:intron 35.0%
! TACGTGTTGGTGCAATATAT+AGG + chr8_1:53340872-53340891 Msa1200600:intron 35.0%
TTAAATCACAGCCACTAATC+AGG + chr8_1:53340937-53340956 Msa1200600:CDS 35.0%
TAATCAGGCAGAAAAAGAGT+TGG + chr8_1:53340952-53340971 Msa1200600:CDS 35.0%
AATCAGGCAGAAAAAGAGTT+GGG + chr8_1:53340953-53340972 Msa1200600:CDS 35.0%
! TGGTGGGGTTGATTATATTA+AGG + chr8_1:53341019-53341038 Msa1200600:CDS 35.0%
GAAGAAATTCCACACTCAAT+CGG + chr8_1:53339795-53339814 Msa1200600:CDS 35.0%
!! TTAATTTGTCCTACTGCTTC+AGG - chr8_1:53339876-53339895 None:intergenic 35.0%
ATCAAATTCGACATTGTCGT+AGG - chr8_1:53339945-53339964 None:intergenic 35.0%
AACTCACATCAAGTTCCTTA+AGG - chr8_1:53339971-53339990 None:intergenic 35.0%
!! TGATGTGAGTTTTAATGAGC+TGG + chr8_1:53339980-53339999 Msa1200600:CDS 35.0%
GTAGCAAAACACAAACTTTC+AGG - chr8_1:53340014-53340033 None:intergenic 35.0%
! TTTTCAACTACATGTCTTGG+AGG - chr8_1:53340224-53340243 None:intergenic 35.0%
TCCAAGACATGTAGTTGAAA+AGG + chr8_1:53340223-53340242 Msa1200600:CDS 35.0%
CCAAGACATGTAGTTGAAAA+GGG + chr8_1:53340224-53340243 Msa1200600:CDS 35.0%
TGCATAATATATGCAAGGTC+CGG + chr8_1:53340633-53340652 Msa1200600:intron 35.0%
!!! TTATTTTTTTTGTGGTGGCC+GGG - chr8_1:53340668-53340687 None:intergenic 35.0%
ATTTAAGAGACACGGCTTAT+CGG + chr8_1:53340715-53340734 Msa1200600:intron 35.0%
! TACGTGTTGGTGCAATATAT+AGG + chr8_1:53340872-53340891 Msa1200600:intron 35.0%
TTAAATCACAGCCACTAATC+AGG + chr8_1:53340937-53340956 Msa1200600:CDS 35.0%
TAATCAGGCAGAAAAAGAGT+TGG + chr8_1:53340952-53340971 Msa1200600:CDS 35.0%
AATCAGGCAGAAAAAGAGTT+GGG + chr8_1:53340953-53340972 Msa1200600:CDS 35.0%
! TGGTGGGGTTGATTATATTA+AGG + chr8_1:53341019-53341038 Msa1200600:CDS 35.0%
ATCGGCACAAAGCTCTTTAA+GGG - chr8_1:53339832-53339851 None:intergenic 40.0%
AATCGGCACAAAGCTCTTTA+AGG - chr8_1:53339833-53339852 None:intergenic 40.0%
!! AGCTTTGTGCCGATTACAAT+CGG + chr8_1:53339838-53339857 Msa1200600:CDS 40.0%
!! AGCTTTGAGCCGATTGTAAT+CGG - chr8_1:53339850-53339869 None:intergenic 40.0%
ACCTCACAGATAGAATCTCA+AGG - chr8_1:53339902-53339921 None:intergenic 40.0%
CTGATATGAGATCATTGCCA+AGG + chr8_1:53340069-53340088 Msa1200600:CDS 40.0%
AGATCATTGCCAAGGTCTAT+TGG + chr8_1:53340077-53340096 Msa1200600:CDS 40.0%
GATCATTGCCAAGGTCTATT+GGG + chr8_1:53340078-53340097 Msa1200600:CDS 40.0%
CCAATTCCTCAAGCATTTCA+AGG - chr8_1:53340106-53340125 None:intergenic 40.0%
CCTTGAAATGCTTGAGGAAT+TGG + chr8_1:53340103-53340122 Msa1200600:CDS 40.0%
!! TAGACTTCGAGTATTGAGAG+TGG + chr8_1:53340178-53340197 Msa1200600:CDS 40.0%
! CCCTTTTCAACTACATGTCT+TGG - chr8_1:53340227-53340246 None:intergenic 40.0%
!! ACAAGTCTGTCTCTAGTTCT+AGG - chr8_1:53340361-53340380 None:intergenic 40.0%
GGACGTGCATAATATATGCA+AGG + chr8_1:53340628-53340647 Msa1200600:intron 40.0%
GCATAATATATGCAAGGTCC+GGG + chr8_1:53340634-53340653 Msa1200600:intron 40.0%
CATAATATATGCAAGGTCCG+GGG + chr8_1:53340635-53340654 Msa1200600:intron 40.0%
!!! TATTTTTTTTGTGGTGGCCG+GGG - chr8_1:53340667-53340686 None:intergenic 40.0%
! AGCACACCCACCAAATATTT+TGG + chr8_1:53340746-53340765 Msa1200600:intron 40.0%
! CTCTTTTTCTGCCTGATTAG+TGG - chr8_1:53340951-53340970 None:intergenic 40.0%
AAGTCTAACACAAGAAAGCG+TGG + chr8_1:53340999-53341018 Msa1200600:CDS 40.0%
ATCGGCACAAAGCTCTTTAA+GGG - chr8_1:53339832-53339851 None:intergenic 40.0%
AATCGGCACAAAGCTCTTTA+AGG - chr8_1:53339833-53339852 None:intergenic 40.0%
!! AGCTTTGTGCCGATTACAAT+CGG + chr8_1:53339838-53339857 Msa1200600:CDS 40.0%
!! AGCTTTGAGCCGATTGTAAT+CGG - chr8_1:53339850-53339869 None:intergenic 40.0%
ACCTCACAGATAGAATCTCA+AGG - chr8_1:53339902-53339921 None:intergenic 40.0%
CTGATATGAGATCATTGCCA+AGG + chr8_1:53340069-53340088 Msa1200600:CDS 40.0%
AGATCATTGCCAAGGTCTAT+TGG + chr8_1:53340077-53340096 Msa1200600:CDS 40.0%
GATCATTGCCAAGGTCTATT+GGG + chr8_1:53340078-53340097 Msa1200600:CDS 40.0%
CCAATTCCTCAAGCATTTCA+AGG - chr8_1:53340106-53340125 None:intergenic 40.0%
CCTTGAAATGCTTGAGGAAT+TGG + chr8_1:53340103-53340122 Msa1200600:CDS 40.0%
!! TAGACTTCGAGTATTGAGAG+TGG + chr8_1:53340178-53340197 Msa1200600:CDS 40.0%
! CCCTTTTCAACTACATGTCT+TGG - chr8_1:53340227-53340246 None:intergenic 40.0%
!! ACAAGTCTGTCTCTAGTTCT+AGG - chr8_1:53340361-53340380 None:intergenic 40.0%
GGACGTGCATAATATATGCA+AGG + chr8_1:53340628-53340647 Msa1200600:intron 40.0%
GCATAATATATGCAAGGTCC+GGG + chr8_1:53340634-53340653 Msa1200600:intron 40.0%
CATAATATATGCAAGGTCCG+GGG + chr8_1:53340635-53340654 Msa1200600:intron 40.0%
!!! TATTTTTTTTGTGGTGGCCG+GGG - chr8_1:53340667-53340686 None:intergenic 40.0%
! AGCACACCCACCAAATATTT+TGG + chr8_1:53340746-53340765 Msa1200600:intron 40.0%
! CTCTTTTTCTGCCTGATTAG+TGG - chr8_1:53340951-53340970 None:intergenic 40.0%
AAGTCTAACACAAGAAAGCG+TGG + chr8_1:53340999-53341018 Msa1200600:CDS 40.0%
CTACAATGACCGATTGAGTG+TGG - chr8_1:53339807-53339826 None:intergenic 45.0%
AAAGCTTTGCCTGAAGCAGT+AGG + chr8_1:53339864-53339883 Msa1200600:CDS 45.0%
GCCTTGAGATTCTATCTGTG+AGG + chr8_1:53339898-53339917 Msa1200600:CDS 45.0%
TCAAGGTTCCCAATAGACCT+TGG - chr8_1:53340089-53340108 None:intergenic 45.0%
TGGGAACCTTGAAATGCTTG+AGG + chr8_1:53340097-53340116 Msa1200600:CDS 45.0%
TGTCTTGGAGGAACTTCAAG+AGG - chr8_1:53340212-53340231 None:intergenic 45.0%
! TGTAGTTGAAAAGGGAGCAC+AGG + chr8_1:53340232-53340251 Msa1200600:CDS 45.0%
! TTTGGTGGGTGTGCTAAGAT+AGG - chr8_1:53340741-53340760 None:intergenic 45.0%
ATAGGCAGTTGTGCAGTACA+TGG + chr8_1:53340890-53340909 Msa1200600:intron 45.0%
ATGGCTGAGCTTGTAGAGAA+AGG + chr8_1:53340909-53340928 Msa1200600:CDS 45.0%
TGGCTGAGCTTGTAGAGAAA+GGG + chr8_1:53340910-53340929 Msa1200600:CDS 45.0%
! TCTAACACAAGAAAGCGTGG+TGG + chr8_1:53341002-53341021 Msa1200600:CDS 45.0%
! CTAACACAAGAAAGCGTGGT+GGG + chr8_1:53341003-53341022 Msa1200600:CDS 45.0%
! TAACACAAGAAAGCGTGGTG+GGG + chr8_1:53341004-53341023 Msa1200600:CDS 45.0%
CTACAATGACCGATTGAGTG+TGG - chr8_1:53339807-53339826 None:intergenic 45.0%
AAAGCTTTGCCTGAAGCAGT+AGG + chr8_1:53339864-53339883 Msa1200600:CDS 45.0%
GCCTTGAGATTCTATCTGTG+AGG + chr8_1:53339898-53339917 Msa1200600:CDS 45.0%
TCAAGGTTCCCAATAGACCT+TGG - chr8_1:53340089-53340108 None:intergenic 45.0%
TGGGAACCTTGAAATGCTTG+AGG + chr8_1:53340097-53340116 Msa1200600:CDS 45.0%
TGTCTTGGAGGAACTTCAAG+AGG - chr8_1:53340212-53340231 None:intergenic 45.0%
! TGTAGTTGAAAAGGGAGCAC+AGG + chr8_1:53340232-53340251 Msa1200600:CDS 45.0%
! TTTGGTGGGTGTGCTAAGAT+AGG - chr8_1:53340741-53340760 None:intergenic 45.0%
ATAGGCAGTTGTGCAGTACA+TGG + chr8_1:53340890-53340909 Msa1200600:intron 45.0%
ATGGCTGAGCTTGTAGAGAA+AGG + chr8_1:53340909-53340928 Msa1200600:CDS 45.0%
TGGCTGAGCTTGTAGAGAAA+GGG + chr8_1:53340910-53340929 Msa1200600:CDS 45.0%
! TCTAACACAAGAAAGCGTGG+TGG + chr8_1:53341002-53341021 Msa1200600:CDS 45.0%
! CTAACACAAGAAAGCGTGGT+GGG + chr8_1:53341003-53341022 Msa1200600:CDS 45.0%
! TAACACAAGAAAGCGTGGTG+GGG + chr8_1:53341004-53341023 Msa1200600:CDS 45.0%
GGCTGAGCTTGTAGAGAAAG+GGG + chr8_1:53340911-53340930 Msa1200600:CDS 50.0%
GGCTGAGCTTGTAGAGAAAG+GGG + chr8_1:53340911-53340930 Msa1200600:CDS 50.0%
AAGGTCCGGGGTACAAACCC+CGG + chr8_1:53340647-53340666 Msa1200600:intron 60.0%
AAGGTCCGGGGTACAAACCC+CGG + chr8_1:53340647-53340666 Msa1200600:intron 60.0%
GGTGGCCGGGGTTTGTACCC+CGG - chr8_1:53340655-53340674 None:intergenic 70.0%
GGTGGCCGGGGTTTGTACCC+CGG - chr8_1:53340655-53340674 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 53339792 53341046 53339792 ID=Msa1200600;Name=Msa1200600
chr8_1 mRNA 53339792 53341046 53339792 ID=Msa1200600-mRNA-1;Parent=Msa1200600;Name=Msa1200600-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|204
chr8_1 exon 53339792 53340253 53339792 ID=Msa1200600-mRNA-1:exon:17047;Parent=Msa1200600-mRNA-1
chr8_1 exon 53340894 53341046 53340894 ID=Msa1200600-mRNA-1:exon:17048;Parent=Msa1200600-mRNA-1
chr8_1 CDS 53339792 53340253 53339792 ID=Msa1200600-mRNA-1:cds;Parent=Msa1200600-mRNA-1
chr8_1 CDS 53340894 53341046 53340894 ID=Msa1200600-mRNA-1:cds;Parent=Msa1200600-mRNA-1
Gene Sequence

>Msa1200600

TTGGAAGAAATTCCACACTCAATCGGTCATTGTAGTTCCCTTAAAGAGCTTTGTGCCGATTACAATCGGCTCAAAGCTTTGCCTGAAGCAGTAGGACAAATTAAGAGCCTTGAGATTCTATCTGTGAGGTACAACAACATTAAACAATTGCCTACGACAATGTCGAATTTGATTAACCTTAAGGAACTTGATGTGAGTTTTAATGAGCTGGAGTTTGTTCCTGAAAGTTTGTGTTTTGCTACTAAGATTGTTAAGATGAATGTTGGGAACAATTTTGCTGATATGAGATCATTGCCAAGGTCTATTGGGAACCTTGAAATGCTTGAGGAATTGGATATTAGTAATAATCAAATACATGCTCTTCCCTATTCTTTTAGAATGCTCACTAGACTTCGAGTATTGAGAGTGGAAGAAAATCCTCTTGAAGTTCCTCCAAGACATGTAGTTGAAAAGGGAGCACAGGCAGTTGTGCAGTACATGGCTGAGCTTGTAGAGAAAGGGGATGTTAAATCACAGCCACTAATCAGGCAGAAAAAGAGTTGGGCTGATCAGATATGCTTCTTTTCAAAGTCTAACACAAGAAAGCGTGGTGGGGTTGATTATATTAAGGCTTGA
Protein sequence

>Msa1200600

LEEIPHSIGHCSSLKELCADYNRLKALPEAVGQIKSLEILSVRYNNIKQLPTTMSNLINLKELDVSFNELEFVPESLCFATKIVKMNVGNNFADMRSLPRSIGNLEMLEELDISNNQIHALPYSFRMLTRLRVLRVEENPLEVPPRHVVEKGAQAVVQYMAELVEKGDVKSQPLIRQKKSWADQICFFSKSNTRKRGGVDYIKA*