Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1218890 | KEH32347.1 | 95.522 | 67 | 3 | 0 | 4 | 70 | 14 | 80 | 7.03e-39 | 133 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1218890 | A0A072URR6 | 95.522 | 67 | 3 | 0 | 4 | 70 | 14 | 80 | 3.36e-39 | 133 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0517720 | Msa1218890 | 0.801813 | 7.542190e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGACCACCACCTTCATTT+TGG | 0.215725 | 8_1:-81573035 | None:intergenic |
| AACTCATGAATGCAAATATA+TGG | 0.283539 | 8_1:+81572994 | Msa1218890:CDS |
| ATAGTGAAATCTCAACAATT+AGG | 0.406454 | 8_1:+81572963 | Msa1218890:CDS |
| TATGGATTACATGTCACTTT+GGG | 0.416476 | 8_1:+81573012 | Msa1218890:CDS |
| CACTGTCTGGCTCAGCACTA+AGG | 0.437237 | 8_1:-81572940 | None:intergenic |
| GAGATTTCACTATCACTGTC+TGG | 0.441520 | 8_1:-81572953 | None:intergenic |
| ATATGGATTACATGTCACTT+TGG | 0.465764 | 8_1:+81573011 | Msa1218890:CDS |
| ACTGTCTGGCTCAGCACTAA+GGG | 0.489883 | 8_1:-81572939 | None:intergenic |
| TCAAATCTGTTGCTTAAAGA+AGG | 0.495285 | 8_1:-81573064 | None:intergenic |
| CAGATGTTGCAGCTTTCAAA+CGG | 0.520476 | 8_1:-81573102 | None:intergenic |
| GTCACTTTGGGCCAAAATGA+AGG | 0.521305 | 8_1:+81573024 | Msa1218890:CDS |
| TTTGCATTCATGAGTTACTT+CGG | 0.543915 | 8_1:-81572987 | None:intergenic |
| GCCAAAATGAAGGTGGTGGT+CGG | 0.582464 | 8_1:+81573034 | Msa1218890:CDS |
| TTGGGCCAAAATGAAGGTGG+TGG | 0.598688 | 8_1:+81573030 | Msa1218890:CDS |
| CTGTTGCTTAAAGAAGGTAG+AGG | 0.637500 | 8_1:-81573058 | None:intergenic |
| TCGTTGAGTTTAAGGCGAGG+CGG | 0.721081 | 8_1:-81573131 | None:intergenic |
| ACTTCGTTGAGTTTAAGGCG+AGG | 0.734213 | 8_1:-81573134 | None:intergenic |
| ACTTTGGGCCAAAATGAAGG+TGG | 0.741537 | 8_1:+81573027 | Msa1218890:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTGATTGTCTAAATTAA+TGG | + | chr8_1:81572821-81572840 | Msa1218890:intron | 15.0% |
| !! | ACAATCAATATAGTTTCATA+TGG | - | chr8_1:81572812-81572831 | None:intergenic | 20.0% |
| ! | GAAATCATATAATGTGCTTA+AGG | - | chr8_1:81572770-81572789 | None:intergenic | 25.0% |
| ! | GCACATTATATGATTTCATT+AGG | + | chr8_1:81572773-81572792 | Msa1218890:intron | 25.0% |
| ! | ATAGTGAAATCTCAACAATT+AGG | + | chr8_1:81572963-81572982 | Msa1218890:CDS | 25.0% |
| ! | AACTCATGAATGCAAATATA+TGG | + | chr8_1:81572994-81573013 | Msa1218890:CDS | 25.0% |
| GAAATAATTGCTGTCATCTT+TGG | - | chr8_1:81572748-81572767 | None:intergenic | 30.0% | |
| AATATAGTTTCATATGGTGC+TGG | - | chr8_1:81572806-81572825 | None:intergenic | 30.0% | |
| TTTGCATTCATGAGTTACTT+CGG | - | chr8_1:81572990-81573009 | None:intergenic | 30.0% | |
| ! | ATATGGATTACATGTCACTT+TGG | + | chr8_1:81573011-81573030 | Msa1218890:CDS | 30.0% |
| !! | TATGGATTACATGTCACTTT+GGG | + | chr8_1:81573012-81573031 | Msa1218890:CDS | 30.0% |
| ! | TCAAATCTGTTGCTTAAAGA+AGG | - | chr8_1:81573067-81573086 | None:intergenic | 30.0% |
| CCTATGTTACATGACGATCT+GGG | + | chr8_1:81572878-81572897 | Msa1218890:intron | 40.0% | |
| GAGATTTCACTATCACTGTC+TGG | - | chr8_1:81572956-81572975 | None:intergenic | 40.0% | |
| ! | CTGTTGCTTAAAGAAGGTAG+AGG | - | chr8_1:81573061-81573080 | None:intergenic | 40.0% |
| CAGATGTTGCAGCTTTCAAA+CGG | - | chr8_1:81573105-81573124 | None:intergenic | 40.0% | |
| GCCTATGTTACATGACGATC+TGG | + | chr8_1:81572877-81572896 | Msa1218890:intron | 45.0% | |
| CCCAGATCGTCATGTAACAT+AGG | - | chr8_1:81572881-81572900 | None:intergenic | 45.0% | |
| ! | GTCACTTTGGGCCAAAATGA+AGG | + | chr8_1:81573024-81573043 | Msa1218890:CDS | 45.0% |
| ACTTTGGGCCAAAATGAAGG+TGG | + | chr8_1:81573027-81573046 | Msa1218890:CDS | 45.0% | |
| AGGGGTCAATACACTTCGCA+AGG | - | chr8_1:81572914-81572933 | None:intergenic | 50.0% | |
| ! | ACTGTCTGGCTCAGCACTAA+GGG | - | chr8_1:81572942-81572961 | None:intergenic | 50.0% |
| TTGGGCCAAAATGAAGGTGG+TGG | + | chr8_1:81573030-81573049 | Msa1218890:CDS | 50.0% | |
| ! | GCCAAAATGAAGGTGGTGGT+CGG | + | chr8_1:81573034-81573053 | Msa1218890:CDS | 50.0% |
| ! | ACCGACCACCACCTTCATTT+TGG | - | chr8_1:81573038-81573057 | None:intergenic | 50.0% |
| TCAGCACTAAGGGCTGTGAG+GGG | - | chr8_1:81572932-81572951 | None:intergenic | 55.0% | |
| CTCAGCACTAAGGGCTGTGA+GGG | - | chr8_1:81572933-81572952 | None:intergenic | 55.0% | |
| ! | CACTGTCTGGCTCAGCACTA+AGG | - | chr8_1:81572943-81572962 | None:intergenic | 55.0% |
| GCTCAGCACTAAGGGCTGTG+AGG | - | chr8_1:81572934-81572953 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 81572667 | 81573144 | 81572667 | ID=Msa1218890;Name=Msa1218890 |
| chr8_1 | mRNA | 81572667 | 81573144 | 81572667 | ID=Msa1218890-mRNA-1;Parent=Msa1218890;Name=Msa1218890-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|0.5|0|0|2|0|70 |
| chr8_1 | exon | 81572667 | 81572673 | 81572667 | ID=Msa1218890-mRNA-1:exon:28903;Parent=Msa1218890-mRNA-1 |
| chr8_1 | exon | 81572939 | 81573144 | 81572939 | ID=Msa1218890-mRNA-1:exon:28904;Parent=Msa1218890-mRNA-1 |
| chr8_1 | CDS | 81572667 | 81572673 | 81572667 | ID=Msa1218890-mRNA-1:cds;Parent=Msa1218890-mRNA-1 |
| chr8_1 | CDS | 81572939 | 81573144 | 81572939 | ID=Msa1218890-mRNA-1:cds;Parent=Msa1218890-mRNA-1 |
| Gene Sequence |
| Protein sequence |
