AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1229910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1229910 MtrunA17_Chr8g0337651 96.344 547 12 1 1 539 1 547 0.0 1096
Msa1229910 MtrunA17_Chr8g0337661 77.697 547 112 2 1 539 1 545 0.0 882
Msa1229910 MtrunA17_Chr4g0053711 37.774 548 287 16 16 532 14 538 3.20e-102 318
Msa1229910 MtrunA17_Chr1g0205391 29.191 507 285 14 34 520 29 481 1.53e-45 167
Msa1229910 MtrunA17_Chr8g0390621 26.872 454 251 9 4 446 3 386 2.86e-36 142
Msa1229910 MtrunA17_Chr3g0101121 32.056 287 174 5 171 452 100 370 1.87e-33 133
Msa1229910 MtrunA17_Chr5g0445281 25.424 472 274 13 38 499 41 444 7.89e-28 117
Msa1229910 MtrunA17_Chr3g0131341 25.606 289 194 8 175 450 155 435 3.03e-18 88.2
Msa1229910 MtrunA17_Chr2g0325711 27.607 163 105 3 263 417 653 810 4.28e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1229910 AT5G48385.1 59.541 566 194 13 1 539 1 558 0.0 599
Msa1229910 AT3G22440.1 28.358 536 310 13 22 531 15 502 7.49e-55 193
Msa1229910 AT4G14900.1 28.102 548 310 15 18 537 11 502 3.72e-50 181
Msa1229910 AT1G31814.1 30.294 340 191 10 158 472 67 385 2.25e-39 150
Msa1229910 AT5G16320.1 30.921 304 177 6 170 463 99 379 3.36e-37 144
Msa1229910 AT5G27230.1 27.344 256 155 5 200 442 566 803 1.08e-16 84.3
Msa1229910 AT5G27220.1 28.070 171 117 2 268 436 918 1084 4.97e-11 66.2
Msa1229910 AT5G27220.3 28.070 171 117 2 268 436 918 1084 4.97e-11 66.2
Msa1229910 AT5G27220.4 30.405 148 96 2 296 436 882 1029 8.44e-11 65.5
Msa1229910 AT5G27220.2 30.405 148 96 2 296 436 882 1029 8.44e-11 65.5

Find 136 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCTGGAAATGGAAATT+TGG 0.146869 8_2:-5855270 Msa1229910:CDS
CTCAGTTACTTAGTTTAATC+AGG 0.214308 8_2:+5856125 None:intergenic
CTAGTTCTTGACTTCAAAAT+AGG 0.248757 8_2:-5856028 Msa1229910:five_prime_UTR
AAGAAGGATGCTAACTTATT+AGG 0.255479 8_2:-5855041 Msa1229910:CDS
GTAAGAACCTTGCTGCTATA+AGG 0.264350 8_2:-5855166 Msa1229910:CDS
TGAAACAACTACTTAATTTC+AGG 0.266984 8_2:+5855791 None:intergenic
GATTTGGCCATTGTCTCTAA+TGG 0.277108 8_2:-5855377 Msa1229910:CDS
TACAGGAGTAATTGAGGTTT+TGG 0.280053 8_2:-5854442 Msa1229910:intron
AGTGATTTCCTGACCAATTC+AGG 0.281957 8_2:-5854981 Msa1229910:CDS
ATTAGGATTAATAATACATT+TGG 0.284565 8_2:+5855866 None:intergenic
ATGTCAAGAGCATCCAATTT+AGG 0.286497 8_2:+5854891 None:intergenic
AATTCCAAATTTCCATTTCC+AGG 0.306679 8_2:+5855266 None:intergenic
CTTACTGAAGTCTTACTTGC+AGG 0.307360 8_2:-5854343 Msa1229910:CDS
ATACATTTGGGGAACTTATA+CGG 0.311707 8_2:+5855879 None:intergenic
GTTGCCTTTGTAGTATGTTT+TGG 0.337561 8_2:-5853435 Msa1229910:intron
ATGTTTAGTGTTAGATTCTT+TGG 0.338403 8_2:-5855102 Msa1229910:CDS
CTGTTACTCTTAACATGAAA+TGG 0.348629 8_2:-5855643 Msa1229910:CDS
ATTTGGCCATTGTCTCTAAT+GGG 0.353906 8_2:-5855376 Msa1229910:CDS
CATACGCATATTGTGTTTGC+GGG 0.356102 8_2:+5853817 None:intergenic
TTAGGATTAATAATACATTT+GGG 0.361004 8_2:+5855867 None:intergenic
CTGCAATGTGGCTTGCTCCT+TGG 0.363624 8_2:+5855478 None:intergenic
TCAACATACAACATCTTAGA+AGG 0.364709 8_2:+5853600 None:intergenic
GTGAAACTTTCTCCTGGAAA+TGG 0.366686 8_2:-5855278 Msa1229910:CDS
CCGTTAGCACGAGGTCTCTT+TGG 0.370940 8_2:+5853912 None:intergenic
ACCAGATGCAATATTAAAAC+TGG 0.378364 8_2:+5854815 None:intergenic
CATGGAAACTACTTTGGAAA+TGG 0.381276 8_2:-5853711 Msa1229910:CDS
ACCCTAAAATTATACATTGC+CGG 0.383344 8_2:-5855972 Msa1229910:five_prime_UTR
CGTGCTAACGGTGTGGGATA+TGG 0.385558 8_2:-5853900 Msa1229910:CDS
TTCACACTCATAATCTGATT+AGG 0.394043 8_2:+5855849 None:intergenic
CATAACCATGGAAACTACTT+TGG 0.397650 8_2:-5853717 Msa1229910:CDS
TGAAGAATGGAAGCCTAAAT+TGG 0.398597 8_2:-5854904 Msa1229910:CDS
ATTAGAAATACCTCCTGAAT+TGG 0.398777 8_2:+5854968 None:intergenic
ATTCTTGCTCACCTGTCAAA+AGG 0.401992 8_2:-5854311 Msa1229910:CDS
TGGTTGAGGCTTTGTCGCTT+CGG 0.407938 8_2:+5853932 None:intergenic
GCAAATTGACGCTGTTAACT+TGG 0.422848 8_2:-5854406 Msa1229910:CDS
TGTAGAGAGATGGATGTCTC+TGG 0.428452 8_2:-5855215 Msa1229910:CDS
TAAGACTTCAGTAAGGAAAC+AGG 0.428714 8_2:+5854351 None:intergenic
AACGGCATAAATCAGAAGTT+TGG 0.431250 8_2:+5854742 None:intergenic
TAAGAACCTTGCTGCTATAA+GGG 0.437970 8_2:-5855165 Msa1229910:CDS
AACACTAAACATGCAGCATT+AGG 0.444227 8_2:+5855113 None:intergenic
CAATGATTGTCAGTTGGTGC+AGG 0.453613 8_2:+5853777 None:intergenic
GACTAGATATATTAAACAAA+AGG 0.453705 8_2:+5853627 None:intergenic
TCTGATTTATGCCGTTGTCT+TGG 0.456620 8_2:-5854735 Msa1229910:CDS
TTATAGCAGCAAGGTTCTTA+CGG 0.464979 8_2:+5855168 None:intergenic
ACATTCCATCAGCATGATAC+AGG 0.465654 8_2:+5855007 None:intergenic
CAAAGGCAGTTGCTGAAGAA+TGG 0.473483 8_2:-5854917 Msa1229910:CDS
AAATACCTCCTGAATTGGTC+AGG 0.475519 8_2:+5854973 None:intergenic
GGCTAATTCCAATGGTATCT+CGG 0.486974 8_2:-5854770 Msa1229910:CDS
GCACGAGGTCTCTTTGGTTG+AGG 0.488064 8_2:+5853918 None:intergenic
GAACTAGAGGAACACTTTCA+CGG 0.491662 8_2:-5855617 Msa1229910:CDS
TTGATGAGGAAGAGTTATCC+AGG 0.497159 8_2:-5854791 Msa1229910:CDS
TGCTAGCAATGGGAATTCCT+TGG 0.499073 8_2:-5854865 Msa1229910:CDS
TCAGATTGAAGTTAACGAAC+GGG 0.499700 8_2:-5854084 Msa1229910:intron
GTAGAGAGATGGATGTCTCT+GGG 0.500705 8_2:-5855214 Msa1229910:CDS
TACTTAATTTCAGGTTGTGA+AGG 0.500939 8_2:+5855800 None:intergenic
CATTTCCAAAGTAGTTTCCA+TGG 0.501736 8_2:+5853712 None:intergenic
TGGACATACAGGAGTAATTG+AGG 0.502419 8_2:-5854448 Msa1229910:intron
TTTAGTATCGAAAGATATCA+AGG 0.507053 8_2:-5854946 Msa1229910:CDS
GAAGATGTGAAACTTTCTCC+TGG 0.508576 8_2:-5855284 Msa1229910:CDS
TCATACGCATATTGTGTTTG+CGG 0.511288 8_2:+5853816 None:intergenic
CTGCATGGAAGTGTCAAGTC+AGG 0.515574 8_2:-5855069 Msa1229910:CDS
TCGTGAAATGTTGAAGAAGC+AGG 0.518564 8_2:-5855516 Msa1229910:CDS
TCAGTTACTTAGTTTAATCA+GGG 0.522501 8_2:+5856126 None:intergenic
GTTGGTGCAGGGTACATGTA+AGG 0.526176 8_2:+5853789 None:intergenic
GGTAAAACTTTGTAGAGAGA+TGG 0.527204 8_2:-5855225 Msa1229910:CDS
AATGTGGAGCGCCGAGGAGA+GGG 0.528563 8_2:+5853751 None:intergenic
CCGGACCTGTATCATGCTGA+TGG 0.529168 8_2:-5855012 Msa1229910:CDS
AGATACCATTGGAATTAGCC+TGG 0.529323 8_2:+5854773 None:intergenic
TTCTTGCTCACCTGTCAAAA+GGG 0.531130 8_2:-5854310 Msa1229910:intron
CCATCAGCATGATACAGGTC+CGG 0.538378 8_2:+5855012 None:intergenic
TTGACATGGATGCTAGCAAT+GGG 0.538859 8_2:-5854875 Msa1229910:CDS
TTAGTGTTAGATTCTTTGGA+AGG 0.539070 8_2:-5855098 Msa1229910:CDS
AGATTCAGTTGCTACACTGA+TGG 0.539537 8_2:-5855729 Msa1229910:CDS
TATCGAAAGATATCAAGGAC+AGG 0.540207 8_2:-5854941 Msa1229910:CDS
GTCAAGTCAGGATATTAAGA+AGG 0.544894 8_2:-5855057 Msa1229910:CDS
TGTGGAGCGCCGAGGAGAGG+GGG 0.547155 8_2:+5853753 None:intergenic
CTAACTTATTAGGTCTTCGC+CGG 0.548484 8_2:-5855031 Msa1229910:CDS
ATTGATATCTTATCCAGAGC+TGG 0.551416 8_2:-5855246 Msa1229910:CDS
GGCTTGAGAAATCTTTGAAG+AGG 0.552611 8_2:-5855595 Msa1229910:CDS
AACTAGAGGAACACTTTCAC+GGG 0.554095 8_2:-5855616 Msa1229910:CDS
TGGCGACGCCGAGATACCAT+TGG 0.556801 8_2:+5854762 None:intergenic
CTCTTGAAGCCTCTGCAATG+TGG 0.561570 8_2:+5855466 None:intergenic
TAGCAATGGGAATTCCTTGG+AGG 0.561927 8_2:-5854862 Msa1229910:CDS
GGATTATCAGAGAAGATGCC+TGG 0.564850 8_2:-5854714 Msa1229910:intron
TATAAATTCTCTCATAACCA+TGG 0.565246 8_2:-5853729 Msa1229910:CDS
TAAATGCTCGAGAAAAGCAC+AGG 0.567504 8_2:-5855415 Msa1229910:CDS
CTGTCGTATCTTCGAAGACG+TGG 0.569615 8_2:+5855703 None:intergenic
ACTAGTGAAGATATGTAGCT+TGG 0.570101 8_2:+5853679 None:intergenic
CCAAAGAGACCTCGTGCTAA+CGG 0.572390 8_2:-5853912 Msa1229910:CDS
TGAACAACTCGTTTCATGAG+AGG 0.576995 8_2:+5853990 None:intergenic
AGCATTTAAAATGGCAAATG+TGG 0.577117 8_2:+5855430 None:intergenic
CAAGAACAGAATTCATACAT+TGG 0.583075 8_2:+5853480 None:intergenic
AGATATCAAGGACAGGGCAA+AGG 0.587264 8_2:-5854934 Msa1229910:CDS
TTTCTTCCCTTATAGCAGCA+AGG 0.588328 8_2:+5855159 None:intergenic
CCTGTCAGCAACTCTAGCAT+AGG 0.590402 8_2:+5853842 None:intergenic
TTTGGCCATTGTCTCTAATG+GGG 0.593249 8_2:-5855375 Msa1229910:CDS
CCTATGCTAGAGTTGCTGAC+AGG 0.595930 8_2:-5853842 Msa1229910:CDS
ATCAGATTGAAGTTAACGAA+CGG 0.596930 8_2:-5854085 Msa1229910:intron
ACGAGTTGTTCAACTAGAGA+AGG 0.598059 8_2:-5853979 Msa1229910:CDS
AGACCTCGTGCTAACGGTGT+GGG 0.598202 8_2:-5853906 Msa1229910:CDS
CTTGACATGGATGCTAGCAA+TGG 0.604007 8_2:-5854876 Msa1229910:CDS
AAGAGTGAATAGAGACACAT+AGG 0.606292 8_2:-5856206 Msa1229910:five_prime_UTR
CATGAAATGGAAAGAACTAG+AGG 0.606972 8_2:-5855630 Msa1229910:CDS
GTTATCCAGGCTAATTCCAA+TGG 0.607375 8_2:-5854778 Msa1229910:CDS
CTGCAAGTAAGACTTCAGTA+AGG 0.609891 8_2:+5854344 None:intergenic
GAGACCTCGTGCTAACGGTG+TGG 0.612459 8_2:-5853907 Msa1229910:CDS
ATCGAAAGATATCAAGGACA+GGG 0.612465 8_2:-5854940 Msa1229910:CDS
TTTCAGTGTCGCATCAGTGT+CGG 0.612995 8_2:+5856344 None:intergenic
TAATGTGGAGCGCCGAGGAG+AGG 0.618224 8_2:+5853750 None:intergenic
TAGGATTAATAATACATTTG+GGG 0.618610 8_2:+5855868 None:intergenic
ACATTAGAAACAGCATCCAT+CGG 0.621125 8_2:+5855323 None:intergenic
TCATGTTAAGAGTAACAGCT+CGG 0.622402 8_2:+5855648 None:intergenic
TTATGAGAGAATTTATAATG+TGG 0.624170 8_2:+5853735 None:intergenic
GGTTGTGAAGGCATGAACCA+TGG 0.627517 8_2:+5855812 None:intergenic
TAAATTGGATGCTCTTGACA+TGG 0.628213 8_2:-5854889 Msa1229910:CDS
GTGCAGGGTACATGTAAGGT+CGG 0.629550 8_2:+5853793 None:intergenic
TTGGCCATTGTCTCTAATGG+GGG 0.630620 8_2:-5855374 Msa1229910:CDS
ATGTGGAGCGCCGAGGAGAG+GGG 0.632547 8_2:+5853752 None:intergenic
AATGATTGTCAGTTGGTGCA+GGG 0.632680 8_2:+5853778 None:intergenic
AGGAGCAAGCCACATTGCAG+AGG 0.633997 8_2:-5855475 Msa1229910:CDS
AGACGTGAAGGAGAAACCGA+TGG 0.634087 8_2:-5855339 Msa1229910:CDS
GAACAGAATTCATACATTGG+AGG 0.636058 8_2:+5853483 None:intergenic
TCATGGTACATCAGACGTGA+AGG 0.636518 8_2:-5855351 Msa1229910:CDS
GAAGATACGACAGCTGCAGA+AGG 0.636526 8_2:-5855693 Msa1229910:CDS
ACAACCAAAACATACTACAA+AGG 0.637634 8_2:+5853431 None:intergenic
CGTTTCATGAGAGGATCTAG+AGG 0.641564 8_2:+5853999 None:intergenic
GTCGTGTCCGATGTTTGTGT+CGG 0.642528 8_2:-5856262 Msa1229910:five_prime_UTR
GCTGTTTCTAATGTGACAGA+AGG 0.646807 8_2:-5855314 Msa1229910:CDS
TGCTCGAGAAAAGCACAGGA+AGG 0.649104 8_2:-5855411 Msa1229910:CDS
ACTAACACCGACACAAACAT+CGG 0.655127 8_2:+5856255 None:intergenic
ATTTATAATGTGGAGCGCCG+AGG 0.659224 8_2:+5853745 None:intergenic
GAAGTGTATACTATTAACCA+TGG 0.681929 8_2:-5855829 Msa1229910:five_prime_UTR
GATTTCCTGACCAATTCAGG+AGG 0.682560 8_2:-5854978 Msa1229910:CDS
TATCCCACACCGTTAGCACG+AGG 0.694652 8_2:+5853903 None:intergenic
TCTCTGATAATCCAAGACAA+CGG 0.703717 8_2:+5854724 None:intergenic
GAAGGAATGTTAGTGACACG+AGG 0.728712 8_2:+5853876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAATTTTTAAATTACTAT+CGG - chr8_2:5853534-5853553 Msa1229910:three_prime_UTR 10.0%
!! TTAGGATTAATAATACATTT+GGG + chr8_2:5853970-5853989 None:intergenic 15.0%
!! ATTAGGATTAATAATACATT+TGG + chr8_2:5853971-5853990 None:intergenic 15.0%
!!! TGTGTGTATAATTTTAGAAA+TGG - chr8_2:5853652-5853671 Msa1229910:three_prime_UTR 20.0%
!! TAGGATTAATAATACATTTG+GGG + chr8_2:5853969-5853988 None:intergenic 20.0%
!! AAAACATCTAATGATCTAAA+TGG - chr8_2:5855256-5855275 Msa1229910:CDS 20.0%
!! TTATGAGAGAATTTATAATG+TGG + chr8_2:5856102-5856121 None:intergenic 20.0%
!! GACTAGATATATTAAACAAA+AGG + chr8_2:5856210-5856229 None:intergenic 20.0%
! TCAGTTACTTAGTTTAATCA+GGG + chr8_2:5853711-5853730 None:intergenic 25.0%
!!! TTTTAGGGTTTTTGAATTTG+AGG + chr8_2:5853851-5853870 None:intergenic 25.0%
! TGAAACAACTACTTAATTTC+AGG + chr8_2:5854046-5854065 None:intergenic 25.0%
! ATGTTTAGTGTTAGATTCTT+TGG - chr8_2:5854732-5854751 Msa1229910:CDS 25.0%
!! TTTAGTATCGAAAGATATCA+AGG - chr8_2:5854888-5854907 Msa1229910:CDS 25.0%
! AGTCTGATATAATTCTCATA+AGG + chr8_2:5855186-5855205 None:intergenic 25.0%
! TTATGAGAATTATATCAGAC+TGG - chr8_2:5855185-5855204 Msa1229910:CDS 25.0%
! TATGAGAATTATATCAGACT+GGG - chr8_2:5855186-5855205 Msa1229910:CDS 25.0%
! TTTCTAATTTAAGCAAGCTA+TGG - chr8_2:5855646-5855665 Msa1229910:CDS 25.0%
! TTCTAATTTAAGCAAGCTAT+GGG - chr8_2:5855647-5855666 Msa1229910:CDS 25.0%
! TATAAATTCTCTCATAACCA+TGG - chr8_2:5856105-5856124 Msa1229910:five_prime_UTR 25.0%
CTCAGTTACTTAGTTTAATC+AGG + chr8_2:5853712-5853731 None:intergenic 30.0%
! CTAGTTCTTGACTTCAAAAT+AGG - chr8_2:5853806-5853825 Msa1229910:CDS 30.0%
ACCCTAAAATTATACATTGC+CGG - chr8_2:5853862-5853881 Msa1229910:CDS 30.0%
ATACATTTGGGGAACTTATA+CGG + chr8_2:5853958-5853977 None:intergenic 30.0%
TTCACACTCATAATCTGATT+AGG + chr8_2:5853988-5854007 None:intergenic 30.0%
GAAGTGTATACTATTAACCA+TGG - chr8_2:5854005-5854024 Msa1229910:CDS 30.0%
!! TACTTAATTTCAGGTTGTGA+AGG + chr8_2:5854037-5854056 None:intergenic 30.0%
CTGTTACTCTTAACATGAAA+TGG - chr8_2:5854191-5854210 Msa1229910:intron 30.0%
! AGCATTTAAAATGGCAAATG+TGG + chr8_2:5854407-5854426 None:intergenic 30.0%
! CTTTTCTCGAGCATTTAAAA+TGG + chr8_2:5854416-5854435 None:intergenic 30.0%
GAAGGTTTCACAAAAAGATT+TGG - chr8_2:5854441-5854460 Msa1229910:intron 30.0%
AATTCCAAATTTCCATTTCC+AGG + chr8_2:5854571-5854590 None:intergenic 30.0%
TTAGTGTTAGATTCTTTGGA+AGG - chr8_2:5854736-5854755 Msa1229910:CDS 30.0%
AAGAAGGATGCTAACTTATT+AGG - chr8_2:5854793-5854812 Msa1229910:CDS 30.0%
ATTAGAAATACCTCCTGAAT+TGG + chr8_2:5854869-5854888 None:intergenic 30.0%
ACCAGATGCAATATTAAAAC+TGG + chr8_2:5855022-5855041 None:intergenic 30.0%
! TAAATGGCTTTTAACATGCA+AGG - chr8_2:5855272-5855291 Msa1229910:CDS 30.0%
!!! TGATGCTTTGTTTTTCGTTT+TGG - chr8_2:5855366-5855385 Msa1229910:CDS 30.0%
!!! TGTTCTTTTAGGGTATGTTT+GGG - chr8_2:5855601-5855620 Msa1229910:CDS 30.0%
ATCTGATACAAGAAGTTACT+AGG + chr8_2:5855736-5855755 None:intergenic 30.0%
ATCAGATTGAAGTTAACGAA+CGG - chr8_2:5855749-5855768 Msa1229910:exon 30.0%
TCAACATACAACATCTTAGA+AGG + chr8_2:5856237-5856256 None:intergenic 30.0%
CAAGAACAGAATTCATACAT+TGG + chr8_2:5856357-5856376 None:intergenic 30.0%
ACAACCAAAACATACTACAA+AGG + chr8_2:5856406-5856425 None:intergenic 30.0%
AAGAGTGAATAGAGACACAT+AGG - chr8_2:5853628-5853647 Msa1229910:three_prime_UTR 35.0%
! GCCGGCAATGTATAATTTTA+GGG + chr8_2:5853866-5853885 None:intergenic 35.0%
!! CTCACGATCTTTTGAAAACA+AGG + chr8_2:5853888-5853907 None:intergenic 35.0%
!!! CTTTTTCAGCTTCTTAGCAA+TGG - chr8_2:5854075-5854094 Msa1229910:CDS 35.0%
TCATGTTAAGAGTAACAGCT+CGG + chr8_2:5854189-5854208 None:intergenic 35.0%
CATGAAATGGAAAGAACTAG+AGG - chr8_2:5854204-5854223 Msa1229910:intron 35.0%
ATTTGGCCATTGTCTCTAAT+GGG - chr8_2:5854458-5854477 Msa1229910:intron 35.0%
ACATTAGAAACAGCATCCAT+CGG + chr8_2:5854514-5854533 None:intergenic 35.0%
TTCTCCTGGAAATGGAAATT+TGG - chr8_2:5854564-5854583 Msa1229910:intron 35.0%
ATTGATATCTTATCCAGAGC+TGG - chr8_2:5854588-5854607 Msa1229910:intron 35.0%
GGTAAAACTTTGTAGAGAGA+TGG - chr8_2:5854609-5854628 Msa1229910:intron 35.0%
TTATAGCAGCAAGGTTCTTA+CGG + chr8_2:5854669-5854688 None:intergenic 35.0%
TAAGAACCTTGCTGCTATAA+GGG - chr8_2:5854669-5854688 Msa1229910:intron 35.0%
AACACTAAACATGCAGCATT+AGG + chr8_2:5854724-5854743 None:intergenic 35.0%
! TTTGGAAGGATTTTACTGCA+TGG - chr8_2:5854750-5854769 Msa1229910:CDS 35.0%
GTCAAGTCAGGATATTAAGA+AGG - chr8_2:5854777-5854796 Msa1229910:CDS 35.0%
TATCGAAAGATATCAAGGAC+AGG - chr8_2:5854893-5854912 Msa1229910:CDS 35.0%
ATCGAAAGATATCAAGGACA+GGG - chr8_2:5854894-5854913 Msa1229910:CDS 35.0%
TGAAGAATGGAAGCCTAAAT+TGG - chr8_2:5854930-5854949 Msa1229910:CDS 35.0%
!! ATGTCAAGAGCATCCAATTT+AGG + chr8_2:5854946-5854965 None:intergenic 35.0%
TAAATTGGATGCTCTTGACA+TGG - chr8_2:5854945-5854964 Msa1229910:CDS 35.0%
!! GCCAGTTTTAATATTGCATC+TGG - chr8_2:5855018-5855037 Msa1229910:CDS 35.0%
!!! TATTGCATCTGGTTTTGATG+AGG - chr8_2:5855029-5855048 Msa1229910:CDS 35.0%
AACGGCATAAATCAGAAGTT+TGG + chr8_2:5855095-5855114 None:intergenic 35.0%
TCTCTGATAATCCAAGACAA+CGG + chr8_2:5855113-5855132 None:intergenic 35.0%
TAAGTGAACAGATACATACC+AGG + chr8_2:5855141-5855160 None:intergenic 35.0%
CAGAACAAACAGATGCAAAT+CGG + chr8_2:5855348-5855367 None:intergenic 35.0%
!! GTTTTTCGTTTTGGACATAC+AGG - chr8_2:5855375-5855394 Msa1229910:CDS 35.0%
! TACAGGAGTAATTGAGGTTT+TGG - chr8_2:5855392-5855411 Msa1229910:CDS 35.0%
TAAGACTTCAGTAAGGAAAC+AGG + chr8_2:5855486-5855505 None:intergenic 35.0%
! TCTTTCCATCGTGTTCTTTT+AGG - chr8_2:5855590-5855609 Msa1229910:CDS 35.0%
! CTTTCCATCGTGTTCTTTTA+GGG - chr8_2:5855591-5855610 Msa1229910:CDS 35.0%
!!! GTGTTCTTTTAGGGTATGTT+TGG - chr8_2:5855600-5855619 Msa1229910:CDS 35.0%
!!! TTTTAGGGTATGTTTGGGAA+GGG - chr8_2:5855606-5855625 Msa1229910:CDS 35.0%
TAAGCAAGCTATGGGTATAT+TGG - chr8_2:5855655-5855674 Msa1229910:CDS 35.0%
!! CTATGGGTATATTGGTCTTA+TGG - chr8_2:5855663-5855682 Msa1229910:CDS 35.0%
TCAGATTGAAGTTAACGAAC+GGG - chr8_2:5855750-5855769 Msa1229910:exon 35.0%
TCATACGCATATTGTGTTTG+CGG + chr8_2:5856021-5856040 None:intergenic 35.0%
CATAACCATGGAAACTACTT+TGG - chr8_2:5856117-5856136 Msa1229910:five_prime_UTR 35.0%
CATTTCCAAAGTAGTTTCCA+TGG + chr8_2:5856125-5856144 None:intergenic 35.0%
! CATGGAAACTACTTTGGAAA+TGG - chr8_2:5856123-5856142 Msa1229910:five_prime_UTR 35.0%
ACTAGTGAAGATATGTAGCT+TGG + chr8_2:5856158-5856177 None:intergenic 35.0%
GAACAGAATTCATACATTGG+AGG + chr8_2:5856354-5856373 None:intergenic 35.0%
! GTTGCCTTTGTAGTATGTTT+TGG - chr8_2:5856399-5856418 Msa1229910:five_prime_UTR 35.0%
ACTAACACCGACACAAACAT+CGG + chr8_2:5853582-5853601 None:intergenic 40.0%
! GGCCGGCAATGTATAATTTT+AGG + chr8_2:5853867-5853886 None:intergenic 40.0%
!! CGATCTTTTGAAAACAAGGC+CGG + chr8_2:5853884-5853903 None:intergenic 40.0%
AGATTCAGTTGCTACACTGA+TGG - chr8_2:5854105-5854124 Msa1229910:intron 40.0%
GAACTAGAGGAACACTTTCA+CGG - chr8_2:5854217-5854236 Msa1229910:intron 40.0%
AACTAGAGGAACACTTTCAC+GGG - chr8_2:5854218-5854237 Msa1229910:intron 40.0%
! GGCTTGAGAAATCTTTGAAG+AGG - chr8_2:5854239-5854258 Msa1229910:intron 40.0%
TCGTGAAATGTTGAAGAAGC+AGG - chr8_2:5854318-5854337 Msa1229910:CDS 40.0%
! TAAATGCTCGAGAAAAGCAC+AGG - chr8_2:5854419-5854438 Msa1229910:CDS 40.0%
GATTTGGCCATTGTCTCTAA+TGG - chr8_2:5854457-5854476 Msa1229910:intron 40.0%
TTTGGCCATTGTCTCTAATG+GGG - chr8_2:5854459-5854478 Msa1229910:intron 40.0%
!! GCTGTTTCTAATGTGACAGA+AGG - chr8_2:5854520-5854539 Msa1229910:intron 40.0%
GAAGATGTGAAACTTTCTCC+TGG - chr8_2:5854550-5854569 Msa1229910:intron 40.0%
GTGAAACTTTCTCCTGGAAA+TGG - chr8_2:5854556-5854575 Msa1229910:intron 40.0%
! CTACAAAGTTTTACCAGCTC+TGG + chr8_2:5854604-5854623 None:intergenic 40.0%
GTAAGAACCTTGCTGCTATA+AGG - chr8_2:5854668-5854687 Msa1229910:intron 40.0%
TTTCTTCCCTTATAGCAGCA+AGG + chr8_2:5854678-5854697 None:intergenic 40.0%
! GGAGAAGCTTTTAATGCATG+AGG + chr8_2:5854703-5854722 None:intergenic 40.0%
CTAACTTATTAGGTCTTCGC+CGG - chr8_2:5854803-5854822 Msa1229910:CDS 40.0%
ACATTCCATCAGCATGATAC+AGG + chr8_2:5854830-5854849 None:intergenic 40.0%
AGTGATTTCCTGACCAATTC+AGG - chr8_2:5854853-5854872 Msa1229910:CDS 40.0%
AAATACCTCCTGAATTGGTC+AGG + chr8_2:5854864-5854883 None:intergenic 40.0%
TTGACATGGATGCTAGCAAT+GGG - chr8_2:5854959-5854978 Msa1229910:CDS 40.0%
TTGATGAGGAAGAGTTATCC+AGG - chr8_2:5855043-5855062 Msa1229910:CDS 40.0%
GTTATCCAGGCTAATTCCAA+TGG - chr8_2:5855056-5855075 Msa1229910:CDS 40.0%
AGATACCATTGGAATTAGCC+TGG + chr8_2:5855064-5855083 None:intergenic 40.0%
GGCTAATTCCAATGGTATCT+CGG - chr8_2:5855064-5855083 Msa1229910:CDS 40.0%
TCTGATTTATGCCGTTGTCT+TGG - chr8_2:5855099-5855118 Msa1229910:CDS 40.0%
TGGACATACAGGAGTAATTG+AGG - chr8_2:5855386-5855405 Msa1229910:CDS 40.0%
GCAAATTGACGCTGTTAACT+TGG - chr8_2:5855428-5855447 Msa1229910:CDS 40.0%
! CAGGAGAAAACTGTTTTGTC+AGG + chr8_2:5855467-5855486 None:intergenic 40.0%
CTGCAAGTAAGACTTCAGTA+AGG + chr8_2:5855493-5855512 None:intergenic 40.0%
CTTACTGAAGTCTTACTTGC+AGG - chr8_2:5855491-5855510 Msa1229910:CDS 40.0%
ATTCTTGCTCACCTGTCAAA+AGG - chr8_2:5855523-5855542 Msa1229910:CDS 40.0%
TTCTTGCTCACCTGTCAAAA+GGG - chr8_2:5855524-5855543 Msa1229910:CDS 40.0%
! GATGAGTTAACCCTTTTGAC+AGG + chr8_2:5855537-5855556 None:intergenic 40.0%
CATACCCTAAAAGAACACGA+TGG + chr8_2:5855598-5855617 None:intergenic 40.0%
!!! CTTTTAGGGTATGTTTGGGA+AGG - chr8_2:5855605-5855624 Msa1229910:CDS 40.0%
! TTTAGGGTATGTTTGGGAAG+GGG - chr8_2:5855607-5855626 Msa1229910:CDS 40.0%
!! TGGGTATATTGGTCTTATGG+AGG - chr8_2:5855666-5855685 Msa1229910:CDS 40.0%
TGAACAACTCGTTTCATGAG+AGG + chr8_2:5855847-5855866 None:intergenic 40.0%
ACGAGTTGTTCAACTAGAGA+AGG - chr8_2:5855855-5855874 Msa1229910:five_prime_UTR 40.0%
AGGCGAAAGCTGACAAAAAA+AGG - chr8_2:5855875-5855894 Msa1229910:five_prime_UTR 40.0%
GGCGAAAGCTGACAAAAAAA+GGG - chr8_2:5855876-5855895 Msa1229910:five_prime_UTR 40.0%
!! GCATAGGAAGTTTTGTCAGA+AGG + chr8_2:5855979-5855998 None:intergenic 40.0%
CATACGCATATTGTGTTTGC+GGG + chr8_2:5856020-5856039 None:intergenic 40.0%
! AATGATTGTCAGTTGGTGCA+GGG + chr8_2:5856059-5856078 None:intergenic 40.0%
TTTCAGTGTCGCATCAGTGT+CGG + chr8_2:5853493-5853512 None:intergenic 45.0%
!!! GGAAGAAGCTGTTTTTGCCA+AGG - chr8_2:5854339-5854358 Msa1229910:CDS 45.0%
TTGGCCATTGTCTCTAATGG+GGG - chr8_2:5854460-5854479 Msa1229910:intron 45.0%
ATGACCCCCATTAGAGACAA+TGG + chr8_2:5854467-5854486 None:intergenic 45.0%
ATTGTCTCTAATGGGGGTCA+TGG - chr8_2:5854466-5854485 Msa1229910:intron 45.0%
TCATGGTACATCAGACGTGA+AGG - chr8_2:5854483-5854502 Msa1229910:intron 45.0%
TGTAGAGAGATGGATGTCTC+TGG - chr8_2:5854619-5854638 Msa1229910:intron 45.0%
GTAGAGAGATGGATGTCTCT+GGG - chr8_2:5854620-5854639 Msa1229910:intron 45.0%
GATTTCCTGACCAATTCAGG+AGG - chr8_2:5854856-5854875 Msa1229910:CDS 45.0%
AGATATCAAGGACAGGGCAA+AGG - chr8_2:5854900-5854919 Msa1229910:CDS 45.0%
CAAAGGCAGTTGCTGAAGAA+TGG - chr8_2:5854917-5854936 Msa1229910:CDS 45.0%
CTTGACATGGATGCTAGCAA+TGG - chr8_2:5854958-5854977 Msa1229910:CDS 45.0%
TGCTAGCAATGGGAATTCCT+TGG - chr8_2:5854969-5854988 Msa1229910:CDS 45.0%
TAGCAATGGGAATTCCTTGG+AGG - chr8_2:5854972-5854991 Msa1229910:CDS 45.0%
TAAAAACGCATGAGCCTCCA+AGG + chr8_2:5854989-5855008 None:intergenic 45.0%
GGATTATCAGAGAAGATGCC+TGG - chr8_2:5855120-5855139 Msa1229910:CDS 45.0%
!! ATTGAGGTTTTGGCGAACAG+TGG - chr8_2:5855402-5855421 Msa1229910:CDS 45.0%
!! TTGAGGTTTTGGCGAACAGT+GGG - chr8_2:5855403-5855422 Msa1229910:CDS 45.0%
CGTTTCATGAGAGGATCTAG+AGG + chr8_2:5855838-5855857 None:intergenic 45.0%
GAAGGAATGTTAGTGACACG+AGG + chr8_2:5855961-5855980 None:intergenic 45.0%
! CAATGATTGTCAGTTGGTGC+AGG + chr8_2:5856060-5856079 None:intergenic 45.0%
!! ATTTATAATGTGGAGCGCCG+AGG + chr8_2:5856092-5856111 None:intergenic 45.0%
GTCGTGTCCGATGTTTGTGT+CGG - chr8_2:5853572-5853591 Msa1229910:three_prime_UTR 50.0%
! GGTTGTGAAGGCATGAACCA+TGG + chr8_2:5854025-5854044 None:intergenic 50.0%
CTGTCGTATCTTCGAAGACG+TGG + chr8_2:5854134-5854153 None:intergenic 50.0%
GAAGATACGACAGCTGCAGA+AGG - chr8_2:5854141-5854160 Msa1229910:intron 50.0%
CTCTTGAAGCCTCTGCAATG+TGG + chr8_2:5854371-5854390 None:intergenic 50.0%
! TGCTCGAGAAAAGCACAGGA+AGG - chr8_2:5854423-5854442 Msa1229910:CDS 50.0%
AGACGTGAAGGAGAAACCGA+TGG - chr8_2:5854495-5854514 Msa1229910:intron 50.0%
!! CTGCATGGAAGTGTCAAGTC+AGG - chr8_2:5854765-5854784 Msa1229910:CDS 50.0%
CCATCAGCATGATACAGGTC+CGG + chr8_2:5854825-5854844 None:intergenic 50.0%
TAACTCATCTCCCACTGCAC+AGG - chr8_2:5855548-5855567 Msa1229910:CDS 50.0%
CAACAACGAACCTGTGCAGT+GGG + chr8_2:5855561-5855580 None:intergenic 50.0%
TCAACAACGAACCTGTGCAG+TGG + chr8_2:5855562-5855581 None:intergenic 50.0%
! TGGTTGAGGCTTTGTCGCTT+CGG + chr8_2:5855905-5855924 None:intergenic 50.0%
CCAAAGAGACCTCGTGCTAA+CGG - chr8_2:5855922-5855941 Msa1229910:five_prime_UTR 50.0%
CCTGTCAGCAACTCTAGCAT+AGG + chr8_2:5855995-5856014 None:intergenic 50.0%
CCTATGCTAGAGTTGCTGAC+AGG - chr8_2:5855992-5856011 Msa1229910:five_prime_UTR 50.0%
GTGCAGGGTACATGTAAGGT+CGG + chr8_2:5856044-5856063 None:intergenic 50.0%
GTTGGTGCAGGGTACATGTA+AGG + chr8_2:5856048-5856067 None:intergenic 50.0%
GGGGGACAATGATTGTCAGT+TGG + chr8_2:5856066-5856085 None:intergenic 50.0%
AATCATTGTCCCCCTCTCCT+CGG - chr8_2:5856072-5856091 Msa1229910:five_prime_UTR 50.0%
!! CTGCAATGTGGCTTGCTCCT+TGG + chr8_2:5854359-5854378 None:intergenic 55.0%
AGGAGCAAGCCACATTGCAG+AGG - chr8_2:5854359-5854378 Msa1229910:CDS 55.0%
CCGGACCTGTATCATGCTGA+TGG - chr8_2:5854822-5854841 Msa1229910:CDS 55.0%
!! AGGTTTTGGCGAACAGTGGG+AGG - chr8_2:5855406-5855425 Msa1229910:CDS 55.0%
GCACGAGGTCTCTTTGGTTG+AGG + chr8_2:5855919-5855938 None:intergenic 55.0%
CCGTTAGCACGAGGTCTCTT+TGG + chr8_2:5855925-5855944 None:intergenic 55.0%
AGACCTCGTGCTAACGGTGT+GGG - chr8_2:5855928-5855947 Msa1229910:five_prime_UTR 55.0%
TATCCCACACCGTTAGCACG+AGG + chr8_2:5855934-5855953 None:intergenic 55.0%
CGTGCTAACGGTGTGGGATA+TGG - chr8_2:5855934-5855953 Msa1229910:five_prime_UTR 55.0%
TGGCGACGCCGAGATACCAT+TGG + chr8_2:5855075-5855094 None:intergenic 60.0%
GAGACCTCGTGCTAACGGTG+TGG - chr8_2:5855927-5855946 Msa1229910:five_prime_UTR 60.0%
!! AATGTGGAGCGCCGAGGAGA+GGG + chr8_2:5856086-5856105 None:intergenic 60.0%
!! TAATGTGGAGCGCCGAGGAG+AGG + chr8_2:5856087-5856106 None:intergenic 60.0%
!! ATGTGGAGCGCCGAGGAGAG+GGG + chr8_2:5856085-5856104 None:intergenic 65.0%
!! TGTGGAGCGCCGAGGAGAGG+GGG + chr8_2:5856084-5856103 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 5853430 5856426 5853430 ID=Msa1229910;Name=Msa1229910
chr8_2 mRNA 5853430 5856426 5853430 ID=Msa1229910-mRNA-1;Parent=Msa1229910;Name=Msa1229910-mRNA-1;_AED=0.26;_eAED=0.26;_QI=664|0.66|0.75|1|0.66|0.5|4|224|539
chr8_2 exon 5853430 5853453 5853430 ID=Msa1229910-mRNA-1:exon:2089;Parent=Msa1229910-mRNA-1
chr8_2 exon 5853480 5854102 5853480 ID=Msa1229910-mRNA-1:exon:2088;Parent=Msa1229910-mRNA-1
chr8_2 exon 5854311 5854459 5854311 ID=Msa1229910-mRNA-1:exon:2087;Parent=Msa1229910-mRNA-1
chr8_2 exon 5854715 5856426 5854715 ID=Msa1229910-mRNA-1:exon:2086;Parent=Msa1229910-mRNA-1
chr8_2 five_prime_UTR 5855763 5856426 5855763 ID=Msa1229910-mRNA-1:five_prime_utr;Parent=Msa1229910-mRNA-1
chr8_2 CDS 5854715 5855762 5854715 ID=Msa1229910-mRNA-1:cds;Parent=Msa1229910-mRNA-1
chr8_2 CDS 5854311 5854459 5854311 ID=Msa1229910-mRNA-1:cds;Parent=Msa1229910-mRNA-1
chr8_2 CDS 5853680 5854102 5853680 ID=Msa1229910-mRNA-1:cds;Parent=Msa1229910-mRNA-1
chr8_2 three_prime_UTR 5853480 5853679 5853480 ID=Msa1229910-mRNA-1:three_prime_utr;Parent=Msa1229910-mRNA-1
chr8_2 three_prime_UTR 5853430 5853453 5853430 ID=Msa1229910-mRNA-1:three_prime_utr;Parent=Msa1229910-mRNA-1
Gene Sequence

>Msa1229910

ATGGAAGAAACAGATTCAGTTGCTACACTGATGGATTCCACGTCTTCGAAGATACGACAGCTGCAGAAGGCATTTGCTGAACTTGAAAGTTACCGAGCTGTTACTCTTAACATGAAATGGAAAGAACTAGAGGAACACTTTCACGGGCTTGAGAAATCTTTGAAGAGGCGCTTTCATGAATTAGAAGATCATGAAAAAGTGTTTGAGAACAAAACTTTGAAAGCTCGTGAAATGTTGAAGAAGCAGGAAGAAGCTGTTTTTGCCAAGGAGCAAGCCACATTGCAGAGGCTTCAAGAGAAAAGAGATGCTGCCACATTTGCCATTTTAAATGCTCGAGAAAAGCACAGGAAGGTTTCACAAAAAGATTTGGCCATTGTCTCTAATGGGGGTCATGGTACATCAGACGTGAAGGAGAAACCGATGGATGCTGTTTCTAATGTGACAGAAGGTCTTGTAGAAGATGTGAAACTTTCTCCTGGAAATGGAAATTTGGAATTGATATCTTATCCAGAGCTGGTAAAACTTTGTAGAGAGATGGATGTCTCTGGGCTTCACAAATTCATATCTGATAACCGTAAGAACCTTGCTGCTATAAGGGAAGAAATACCTCATGCATTAAAAGCTTCTCCTAATGCTGCATGTTTAGTGTTAGATTCTTTGGAAGGATTTTACTGCATGGAAGTGTCAAGTCAGGATATTAAGAAGGATGCTAACTTATTAGGTCTTCGCCGGACCTGTATCATGCTGATGGAATGTCTGAGTGATTTCCTGACCAATTCAGGAGGTATTTCTAATTTAGTATCGAAAGATATCAAGGACAGGGCAAAGGCAGTTGCTGAAGAATGGAAGCCTAAATTGGATGCTCTTGACATGGATGCTAGCAATGGGAATTCCTTGGAGGCTCATGCGTTTTTACAACTTCTAGCCAGTTTTAATATTGCATCTGGTTTTGATGAGGAAGAGTTATCCAGGCTAATTCCAATGGTATCTCGGCGTCGCCAAACTTCTGATTTATGCCGTTGTCTTGGATTATCAGAGAAGATGCCTGGAGTAATTGAGGTTTTGGCGAACAGTGGGAGGCAAATTGACGCTGTTAACTTGGCTTTCGCATTTGACCTGACAAAACAGTTTTCTCCTGTTTCCTTACTGAAGTCTTACTTGCAGGATGCTAGAAATTCTTGCTCACCTGTCAAAAGGATTGAAGTTAACGAACGGGAGCTAATCGCTCATAAAGCAGTAATCAAGTGCATTGAAGAACGTAAACTTGATGAGCAGTATCCTCTAGATCCTCTCATGAAACGAGTTGTTCAACTAGAGAAGGCGAAAGCTGACAAAAAAAGGGAAACCGAAGCGACAAAGCCTCAACCAAAGAGACCTCGTGCTAACGGTGTGGGATATGGTCCTCGTGTCACTAACATTCCTTCTGACAAAACTTCCTATGCTAGAGTTGCTGACAGGCACCCGCAAACACAATATGCGTATGACCGACCTTACATGTACCCTGCACCAACTGACAATCATTGTCCCCCTCTCCTCGGCGCTCCACATTATAAATTCTCTCATAACCATGGAAACTACTTTGGAAATGGCTATCAGTACCAAGCTACATATCTTCACTAG
Protein sequence

>Msa1229910

MEETDSVATLMDSTSSKIRQLQKAFAELESYRAVTLNMKWKELEEHFHGLEKSLKRRFHELEDHEKVFENKTLKAREMLKKQEEAVFAKEQATLQRLQEKRDAATFAILNAREKHRKVSQKDLAIVSNGGHGTSDVKEKPMDAVSNVTEGLVEDVKLSPGNGNLELISYPELVKLCREMDVSGLHKFISDNRKNLAAIREEIPHALKASPNAACLVLDSLEGFYCMEVSSQDIKKDANLLGLRRTCIMLMECLSDFLTNSGGISNLVSKDIKDRAKAVAEEWKPKLDALDMDASNGNSLEAHAFLQLLASFNIASGFDEEELSRLIPMVSRRRQTSDLCRCLGLSEKMPGVIEVLANSGRQIDAVNLAFAFDLTKQFSPVSLLKSYLQDARNSCSPVKRIEVNERELIAHKAVIKCIEERKLDEQYPLDPLMKRVVQLEKAKADKKRETEATKPQPKRPRANGVGYGPRVTNIPSDKTSYARVADRHPQTQYAYDRPYMYPAPTDNHCPPLLGAPHYKFSHNHGNYFGNGYQYQATYLH*