AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1232190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1232190 MtrunA17_Chr8g0341111 86.441 236 32 0 1 236 6 241 1.25e-150 438
Msa1232190 MtrunA17_Chr8g0341061 83.613 238 39 0 2 239 11 248 2.00e-144 421
Msa1232190 MtrunA17_Chr8g0341091 79.498 239 47 1 1 239 6 242 8.66e-136 395
Msa1232190 MtrunA17_Chr8g0341101 70.763 236 69 0 1 236 9 244 3.13e-111 340
Msa1232190 MtrunA17_Chr2g0330611 41.885 191 96 3 44 232 22 199 6.91e-41 140
Msa1232190 MtrunA17_Chr5g0423881 42.623 183 102 3 30 209 42 224 1.31e-40 144
Msa1232190 MtrunA17_Chr2g0330591 41.969 193 94 4 44 232 22 200 1.49e-37 139
Msa1232190 MtrunA17_Chr6g0487561 43.915 189 91 5 50 232 37 216 2.60e-37 134
Msa1232190 MtrunA17_Chr8g0340931 41.667 192 99 4 43 232 22 202 7.97e-37 137
Msa1232190 MtrunA17_Chr8g0340881 42.708 192 96 4 43 232 26 205 9.41e-37 137
Msa1232190 MtrunA17_Chr6g0487571 43.005 193 93 6 47 232 25 207 3.80e-36 135
Msa1232190 MtrunA17_Chr8g0340871 40.625 192 101 4 43 232 28 208 1.87e-34 130
Msa1232190 MtrunA17_Chr8g0341231 37.179 234 122 6 1 232 11 221 2.49e-34 129
Msa1232190 MtrunA17_Chr8g0340891 40.104 192 102 4 43 232 26 206 1.13e-33 128
Msa1232190 MtrunA17_Chr8g0340941 43.787 169 86 4 43 208 19 181 1.20e-33 127
Msa1232190 MtrunA17_Chr8g0341011 41.714 175 93 3 43 215 26 193 1.35e-33 127
Msa1232190 MtrunA17_Chr8g0347661 39.583 192 100 6 43 231 26 204 1.95e-33 127
Msa1232190 MtrunA17_Chr8g0341351 38.424 203 112 4 8 208 5 196 5.86e-33 123
Msa1232190 MtrunA17_Chr8g0340911 40.223 179 97 3 43 219 26 196 1.18e-32 125
Msa1232190 MtrunA17_Chr8g0341151 41.420 169 90 4 43 208 16 178 3.73e-32 124
Msa1232190 MtrunA17_Chr8g0341141 40.104 192 102 5 43 232 21 201 1.65e-31 120
Msa1232190 MtrunA17_Chr8g0358831 41.317 167 90 4 43 206 34 195 6.34e-31 120
Msa1232190 MtrunA17_Chr8g0340961 36.269 193 88 5 43 232 22 182 9.75e-27 105
Msa1232190 MtrunA17_Chr8g0341271 41.791 134 71 2 99 232 6 132 3.07e-24 98.2
Msa1232190 MtrunA17_Chr6g0479311 37.654 162 87 6 51 210 26 175 1.02e-21 93.2
Msa1232190 MtrunA17_Chr7g0263591 38.650 163 90 6 50 211 32 185 6.78e-21 91.3
Msa1232190 MtrunA17_Chr1g0181731 36.196 163 90 5 49 208 25 176 6.14e-20 88.6
Msa1232190 MtrunA17_Chr8g0341201 48.315 89 46 0 127 215 23 111 6.18e-20 83.6
Msa1232190 MtrunA17_Chr3g0123141 32.298 161 98 5 51 208 25 177 6.86e-16 76.6
Msa1232190 MtrunA17_Chr7g0236881 30.570 193 119 9 43 232 19 199 1.27e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1232190 AT4G29990.1 42.021 188 97 3 47 232 23 200 1.16e-39 145
Msa1232190 AT4G29990.2 42.021 188 97 3 47 232 23 200 1.52e-39 145
Msa1232190 AT1G05700.1 46.914 162 75 3 47 205 27 180 3.68e-38 141
Msa1232190 AT1G05700.2 46.914 162 75 3 47 205 27 180 4.14e-38 141
Msa1232190 AT4G29180.1 46.667 165 85 2 50 211 27 191 4.24e-38 141
Msa1232190 AT4G29180.3 46.667 165 85 2 50 211 27 191 5.29e-38 140
Msa1232190 AT4G29180.2 46.667 165 85 2 50 211 27 191 5.67e-38 140
Msa1232190 AT2G19230.1 40.722 194 103 4 43 234 22 205 1.08e-37 140
Msa1232190 AT2G19230.2 40.722 194 103 4 43 234 22 205 1.42e-37 139
Msa1232190 AT5G59650.1 45.732 164 76 4 51 208 29 185 2.42e-37 139
Msa1232190 AT5G59650.2 45.732 164 76 4 51 208 29 185 2.60e-37 139
Msa1232190 AT3G46330.1 44.242 165 78 4 50 208 30 186 1.97e-36 136
Msa1232190 AT3G46350.1 43.605 172 86 4 43 208 22 188 2.04e-36 133
Msa1232190 AT3G46330.2 44.242 165 78 4 50 208 30 186 2.40e-36 136
Msa1232190 AT2G19210.1 39.583 192 104 3 43 232 23 204 5.11e-36 135
Msa1232190 AT3G46420.1 41.624 197 96 5 19 211 7 188 1.94e-35 133
Msa1232190 AT2G28990.2 41.279 172 93 3 43 211 17 183 3.37e-35 132
Msa1232190 AT2G28990.1 41.279 172 93 3 43 211 17 183 3.37e-35 132
Msa1232190 AT4G29450.1 44.186 172 93 3 43 211 19 190 5.10e-35 132
Msa1232190 AT1G51870.2 39.196 199 100 4 13 208 4 184 5.10e-35 132
Msa1232190 AT1G51870.1 39.196 199 100 4 13 208 4 184 5.98e-35 132
Msa1232190 AT5G16900.1 43.452 168 85 4 50 213 23 184 8.34e-35 132
Msa1232190 AT5G16900.3 43.452 168 85 4 50 213 23 184 8.34e-35 132
Msa1232190 AT5G16900.2 43.452 168 85 4 50 213 23 184 9.68e-35 131
Msa1232190 AT1G51890.1 42.353 170 88 4 43 208 17 180 2.01e-34 130
Msa1232190 AT1G51860.1 42.697 178 92 4 38 211 16 187 2.02e-34 130
Msa1232190 AT1G51890.2 42.353 170 88 4 43 208 17 180 2.06e-34 130
Msa1232190 AT1G51890.3 42.353 170 88 4 43 208 17 180 2.57e-34 130
Msa1232190 AT5G59680.1 41.104 163 87 4 50 208 28 185 2.93e-34 130
Msa1232190 AT2G04300.1 45.143 175 84 5 42 211 25 192 4.59e-34 129
Msa1232190 AT2G04300.2 45.143 175 84 5 42 211 25 192 5.54e-34 129
Msa1232190 AT1G07560.1 40.449 178 94 4 39 211 18 188 5.99e-34 129
Msa1232190 AT1G51840.2 42.424 165 83 4 50 211 32 187 7.34e-34 121
Msa1232190 AT1G51840.5 42.424 165 83 4 50 211 32 187 7.34e-34 121
Msa1232190 AT1G51840.4 42.424 165 83 4 50 211 32 187 7.34e-34 121
Msa1232190 AT1G51840.3 42.424 165 83 4 50 211 32 187 7.34e-34 121
Msa1232190 AT1G51840.1 42.424 165 83 4 50 211 32 187 7.34e-34 121
Msa1232190 AT1G49100.1 39.796 196 95 4 16 208 10 185 9.75e-34 129
Msa1232190 AT3G46400.1 41.463 164 86 4 50 208 27 185 1.31e-33 128
Msa1232190 AT3G21340.1 44.242 165 84 3 50 211 32 191 2.98e-33 127
Msa1232190 AT3G21340.2 44.242 165 84 3 50 211 32 191 3.23e-33 127
Msa1232190 AT1G51910.1 44.242 165 84 3 50 211 28 187 4.74e-33 126
Msa1232190 AT5G59670.2 41.379 174 87 6 42 208 18 183 1.25e-32 125
Msa1232190 AT5G59670.1 41.379 174 87 6 42 208 18 183 1.25e-32 125
Msa1232190 AT1G51800.1 41.885 191 93 6 47 234 25 200 4.25e-32 124
Msa1232190 AT1G51850.2 43.030 165 82 4 50 211 25 180 9.96e-32 122
Msa1232190 AT1G51880.2 41.566 166 87 4 50 211 46 205 1.04e-31 122
Msa1232190 AT1G51880.3 41.566 166 87 4 50 211 46 205 1.13e-31 122
Msa1232190 AT1G51880.1 41.566 166 87 4 50 211 28 187 1.27e-31 122
Msa1232190 AT2G29000.1 41.379 174 89 6 42 211 20 184 1.50e-31 122
Msa1232190 AT2G28960.2 35.981 214 106 6 1 211 1 186 4.98e-31 120
Msa1232190 AT1G51790.2 42.424 165 87 3 50 211 28 187 7.00e-31 120
Msa1232190 AT1G51790.3 42.424 165 87 3 50 211 28 187 7.32e-31 120
Msa1232190 AT1G51790.1 42.424 165 87 3 50 211 28 187 7.32e-31 120
Msa1232190 AT4G20450.2 39.500 200 96 9 50 228 29 224 2.08e-30 119
Msa1232190 AT4G20450.1 39.500 200 96 9 50 228 29 224 2.26e-30 119
Msa1232190 AT1G07550.1 39.766 171 91 4 38 203 16 179 2.90e-30 118
Msa1232190 AT2G19190.1 38.596 171 97 3 43 209 22 188 6.44e-30 117
Msa1232190 AT2G14510.1 34.158 202 107 6 11 208 7 186 1.13e-29 117
Msa1232190 AT2G14510.2 34.158 202 107 6 11 208 15 194 1.17e-29 117
Msa1232190 AT1G51805.2 40.341 176 79 5 43 208 18 177 2.19e-29 116
Msa1232190 AT1G51805.1 40.341 176 79 5 43 208 18 177 2.38e-29 116
Msa1232190 AT1G51820.1 42.424 165 83 4 50 211 25 180 3.20e-29 115
Msa1232190 AT3G46340.1 37.278 169 97 3 44 208 25 188 3.13e-28 112
Msa1232190 AT2G14440.1 34.466 206 102 7 11 208 7 187 4.54e-27 109
Msa1232190 AT1G51850.1 38.788 165 69 4 50 211 25 160 3.35e-24 101
Msa1232190 AT3G46370.2 49.462 93 44 2 112 203 2 92 9.44e-21 91.3
Msa1232190 AT3G46370.1 49.462 93 44 2 112 203 2 92 9.44e-21 91.3
Msa1232190 AT2G37050.3 36.527 167 95 6 44 208 21 178 9.64e-21 91.3
Msa1232190 AT2G37050.1 36.527 167 95 6 44 208 21 178 9.82e-21 91.3
Msa1232190 AT2G37050.2 36.527 167 95 6 44 208 21 178 1.04e-20 90.9
Msa1232190 AT2G37050.4 36.527 167 95 6 44 208 21 178 1.07e-20 90.9
Msa1232190 AT3G46270.2 34.545 165 93 6 52 210 38 193 1.66e-19 87.0
Msa1232190 AT3G46270.1 34.545 165 93 6 52 210 30 185 1.75e-19 87.0
Msa1232190 AT3G46260.1 36.747 166 89 7 52 210 29 185 9.31e-19 84.7
Msa1232190 AT3G46280.1 36.145 166 89 6 52 210 25 180 1.21e-18 84.7
Msa1232190 AT3G46240.1 35.036 137 81 3 52 188 6 134 1.71e-17 81.3
Msa1232190 AT1G24485.4 33.333 186 114 6 27 210 4 181 5.56e-16 77.0
Msa1232190 AT1G24485.1 33.333 186 114 6 27 210 4 181 6.48e-16 76.6
Msa1232190 AT1G24485.2 35.152 165 97 6 48 210 25 181 7.67e-16 76.6
Msa1232190 AT1G24485.3 33.333 186 114 6 27 210 4 181 9.39e-16 76.3
Msa1232190 AT3G19230.1 33.714 175 95 8 50 215 22 184 2.86e-14 72.0
Msa1232190 AT1G67720.1 30.189 159 103 4 51 208 26 177 7.99e-14 70.9
Msa1232190 AT5G59660.2 47.619 63 31 1 103 165 41 101 2.71e-11 63.5

Find 68 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCAGTTTCTATCTCCTTC+TGG 0.190843 8_2:+8460230 None:intergenic
TTCAAGATGATACCGGAATT+TGG 0.225860 8_2:-8460364 Msa1232190:CDS
GCTTGACTATTGCAAATAAA+TGG 0.233035 8_2:+8462407 None:intergenic
TATCTGTTGATTCTGATAAA+TGG 0.234928 8_2:+8459935 None:intergenic
TACTATTTATTACTCTTATA+CGG 0.253645 8_2:-8460063 Msa1232190:CDS
AATTCCTTGCATTTCTTCAT+TGG 0.272462 8_2:-8459979 Msa1232190:CDS
CTTATAAATAAGTCTATAAT+AGG 0.305087 8_2:+8459712 None:intergenic
TGTATCTTGGAGTTGATCTT+TGG 0.307863 8_2:-8460106 Msa1232190:CDS
TTTGTCTAGTTGTTCTGAAT+TGG 0.320853 8_2:+8462385 None:intergenic
TTACTGGAAATTATATATAT+AGG 0.322385 8_2:+8462712 None:intergenic
TAACATAAAGATGACTATCT+TGG 0.322580 8_2:+8462457 None:intergenic
TACTGGAAATTATATATATA+GGG 0.343762 8_2:+8462713 None:intergenic
CAACATTGGTTGTGGATCAT+TGG 0.361589 8_2:+8459905 None:intergenic
CAATGTTGGAAATGGATGTT+AGG 0.366840 8_2:-8459890 Msa1232190:CDS
GAGTCGAGTTAGGATTTATA+AGG 0.368940 8_2:-8459758 Msa1232190:three_prime_UTR
TGGGAATTTCGAGTCGAGTT+AGG 0.376880 8_2:-8459768 Msa1232190:three_prime_UTR
GACGAAGGGTTTGTGGAAAC+AGG 0.382152 8_2:-8460332 Msa1232190:CDS
GAAGAAATGCAAGGAATTGT+TGG 0.396607 8_2:+8459984 None:intergenic
CTGTTTGTATTCGCAGAACT+TGG 0.397616 8_2:-8462602 Msa1232190:five_prime_UTR
TGATGATTACTTGGTTGAAC+AGG 0.407678 8_2:+8459848 None:intergenic
AGGATAGTTTAGGTTGATAG+TGG 0.423035 8_2:+8460289 None:intergenic
TCCCAACGTAAGGATAGTTT+AGG 0.426555 8_2:+8460279 None:intergenic
TCCATTTCCAACATTGGTTG+TGG 0.426966 8_2:+8459897 None:intergenic
AACATAAAGATGACTATCTT+GGG 0.434653 8_2:+8462458 None:intergenic
TTATAAGGTTAATGTCTTGT+TGG 0.439510 8_2:-8459743 Msa1232190:three_prime_UTR
TCCACAACCAATGTTGGAAA+TGG 0.440606 8_2:-8459898 Msa1232190:CDS
TATATATATAATTTCCAGTA+AGG 0.442714 8_2:-8462710 Msa1232190:five_prime_UTR
TATATATAGGGACAGGTCAC+AGG 0.453700 8_2:+8462725 None:intergenic
ATAATAAAGCATTGTAATCA+AGG 0.456485 8_2:+8459822 None:intergenic
ACCTAAACTATCCTTACGTT+GGG 0.469535 8_2:-8460280 Msa1232190:CDS
GTTAATTGTTTCCCAACGTA+AGG 0.473123 8_2:+8460269 None:intergenic
AGCAGTTTCTATCTCCTTCT+GGG 0.473728 8_2:+8460231 None:intergenic
TAAAACAGACGAAGGGTTTG+TGG 0.479119 8_2:-8460339 Msa1232190:CDS
TCAAGGTGTTGATGATTACT+TGG 0.481257 8_2:+8459839 None:intergenic
CTAACATCCATTTCCAACAT+TGG 0.483680 8_2:+8459891 None:intergenic
ACATTTATAGTGTCAGTTGA+AGG 0.493589 8_2:+8460026 None:intergenic
TTCCTTGCAGACAGATTACA+TGG 0.496809 8_2:-8462492 Msa1232190:exon
CATGTCAAAGTGTAATGAGT+AGG 0.498566 8_2:-8459793 Msa1232190:three_prime_UTR
GATTACTTTCAAGATGATAC+CGG 0.513295 8_2:-8460371 Msa1232190:CDS
TCGTGAGTTCGTATATGTCA+TGG 0.529394 8_2:-8462556 Msa1232190:five_prime_UTR
AACCTAAACTATCCTTACGT+TGG 0.532473 8_2:-8460281 Msa1232190:CDS
GAAATTATATATATAGGGAC+AGG 0.555025 8_2:+8462718 None:intergenic
CAAAGTGTAATGAGTAGGGG+TGG 0.555966 8_2:-8459788 Msa1232190:three_prime_UTR
CAATGATCCACAACCAATGT+TGG 0.556170 8_2:-8459904 Msa1232190:CDS
TTACTTGGTTGAACAGGGCG+AGG 0.557991 8_2:+8459854 None:intergenic
ATGTCAAAGTGTAATGAGTA+GGG 0.572602 8_2:-8459792 Msa1232190:three_prime_UTR
TACTTGGTTGAACAGGGCGA+GGG 0.573954 8_2:+8459855 None:intergenic
TTCCATGTAATCTGTCTGCA+AGG 0.578350 8_2:+8462490 None:intergenic
AATGTGTGCCTTGTGAAGAC+TGG 0.580671 8_2:-8460008 Msa1232190:CDS
TACACATTGAAACCAAAAGA+AGG 0.581443 8_2:-8460209 Msa1232190:CDS
AGCGTGCGTAGTGCTTGCCT+TGG 0.582155 8_2:-8462432 Msa1232190:CDS
AAAGTGTAATGAGTAGGGGT+GGG 0.589814 8_2:-8459787 Msa1232190:three_prime_UTR
ATTGCTAACAACGAAAATCC+AGG 0.593689 8_2:-8462347 Msa1232190:intron
TATATATATACAAGGACAAG+TGG 0.596739 8_2:+8462645 None:intergenic
ATGGATGTTAGGTTCGATGT+TGG 0.598239 8_2:-8459879 Msa1232190:CDS
ATATATATAATTTCCAGTAA+GGG 0.603085 8_2:-8462709 Msa1232190:five_prime_UTR
ACATTGAGATGTTTCCCAGA+AGG 0.609996 8_2:-8460245 Msa1232190:CDS
TTTGGTATAAAACAGACGAA+GGG 0.616236 8_2:-8460346 Msa1232190:CDS
ACTTGGTTGAACAGGGCGAG+GGG 0.616784 8_2:+8459856 None:intergenic
ATTTGGTATAAAACAGACGA+AGG 0.617389 8_2:-8460347 Msa1232190:CDS
TACATTGGTATATATATACA+AGG 0.619664 8_2:+8462637 None:intergenic
TTGTTGGACCAGTCTTCACA+AGG 0.630039 8_2:+8460000 None:intergenic
AATTCCAATGAAGAAATGCA+AGG 0.633362 8_2:+8459975 None:intergenic
GATGATTACTTGGTTGAACA+GGG 0.637683 8_2:+8459849 None:intergenic
ATATATAGGGACAGGTCACA+GGG 0.640525 8_2:+8462726 None:intergenic
ATATAATTTCCAGTAAGGGA+CGG 0.656484 8_2:-8462705 Msa1232190:five_prime_UTR
ATTGCAAATAAATGGCACCA+AGG 0.700311 8_2:+8462415 None:intergenic
TGTCAAAGTGTAATGAGTAG+GGG 0.740378 8_2:-8459791 Msa1232190:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTAAATACTTTTAAAT+AGG - chr8_2:8461426-8461445 Msa1232190:intron 10.0%
!!! TTAAAATATTCATGATTTTA+TGG + chr8_2:8461568-8461587 None:intergenic 10.0%
!!! AAATCATGAATATTTTAAAA+AGG - chr8_2:8461570-8461589 Msa1232190:intron 10.0%
!! TACTGGAAATTATATATATA+GGG + chr8_2:8459680-8459699 None:intergenic 15.0%
!! TTACTGGAAATTATATATAT+AGG + chr8_2:8459681-8459700 None:intergenic 15.0%
!! TATATATATAATTTCCAGTA+AGG - chr8_2:8459680-8459699 Msa1232190:three_prime_UTR 15.0%
!! ATATATATAATTTCCAGTAA+GGG - chr8_2:8459681-8459700 Msa1232190:three_prime_UTR 15.0%
!! ATATATACCTACTATAATTA+TGG - chr8_2:8460297-8460316 Msa1232190:CDS 15.0%
!!! CACAAAGTAATTTTTTTTAA+AGG + chr8_2:8460420-8460439 None:intergenic 15.0%
!! AGAGACAATAATAATAATAT+GGG - chr8_2:8460554-8460573 Msa1232190:intron 15.0%
!!! AGGAAAGATAAATATTTTTA+GGG + chr8_2:8460759-8460778 None:intergenic 15.0%
!!! AAGGAAAGATAAATATTTTT+AGG + chr8_2:8460760-8460779 None:intergenic 15.0%
!!! AATGTACAATATTGATTTAT+TGG - chr8_2:8460902-8460921 Msa1232190:intron 15.0%
!!! GAGAAAAAATAGATTTTTTT+TGG + chr8_2:8461023-8461042 None:intergenic 15.0%
!!! GAACTCATTTTTTTAAAAAA+TGG - chr8_2:8461207-8461226 Msa1232190:intron 15.0%
!! TTCCCTAAAAAAAAAAAAAA+TGG - chr8_2:8461462-8461481 Msa1232190:intron 15.0%
!!! GACCATTTTTTTTTTTTTTA+GGG + chr8_2:8461467-8461486 None:intergenic 15.0%
!!! ATAGTGATTGTTATTATATA+AGG + chr8_2:8461665-8461684 None:intergenic 15.0%
!! ATAGTAAATAGTTAAAGATA+GGG + chr8_2:8461697-8461716 None:intergenic 15.0%
!! GTAATGTATAATTAACTTAT+AGG - chr8_2:8461965-8461984 Msa1232190:intron 15.0%
!!! TTTTTATTTTTTCCTTCTTT+TGG + chr8_2:8462196-8462215 None:intergenic 15.0%
!! TACTATTTATTACTCTTATA+CGG - chr8_2:8462327-8462346 Msa1232190:intron 15.0%
!! CTTATAAATAAGTCTATAAT+AGG + chr8_2:8462681-8462700 None:intergenic 15.0%
!!! TGAAATATAAGTTTTTAAGT+AGG + chr8_2:8462745-8462764 None:intergenic 15.0%
!! TTGACGTCAAAAAAAAAAAA+AGG + chr8_2:8459715-8459734 None:intergenic 20.0%
!!! TTTTTTTTTTGACGTCAAAA+AGG - chr8_2:8459717-8459736 Msa1232190:three_prime_UTR 20.0%
!! TACATTGGTATATATATACA+AGG + chr8_2:8459756-8459775 None:intergenic 20.0%
!! ATAATCAAGTAAGCAAAATA+AGG - chr8_2:8460099-8460118 Msa1232190:CDS 20.0%
!! TAATCAAGTAAGCAAAATAA+GGG - chr8_2:8460100-8460119 Msa1232190:CDS 20.0%
!!! AATCATAATGTTTAGGATAT+TGG - chr8_2:8460138-8460157 Msa1232190:CDS 20.0%
!!! GTTTAAGAAACTACAAATTT+TGG - chr8_2:8460160-8460179 Msa1232190:CDS 20.0%
!! AAAGTTACCATAATTATAGT+AGG + chr8_2:8460307-8460326 None:intergenic 20.0%
!! AAAAAAATTACTTTGTGAAG+AGG - chr8_2:8460423-8460442 Msa1232190:intron 20.0%
!!! TTTTTTGTCAAGTAATTTAG+TGG - chr8_2:8460467-8460486 Msa1232190:intron 20.0%
!! CAGAGACAATAATAATAATA+TGG - chr8_2:8460553-8460572 Msa1232190:intron 20.0%
!!! ATGGTTATAATTTCCTTTTT+TGG + chr8_2:8460618-8460637 None:intergenic 20.0%
!!! ACTATATAATTTCCGTTTTT+AGG - chr8_2:8460696-8460715 Msa1232190:intron 20.0%
!! GAGAATATTTCAATAAAACT+AGG + chr8_2:8460872-8460891 None:intergenic 20.0%
!!! TCATTTTTAAGTGTTTGTTT+AGG - chr8_2:8461061-8461080 Msa1232190:intron 20.0%
!!! ATCTATTTTTTTCAACAACA+AGG - chr8_2:8461163-8461182 Msa1232190:intron 20.0%
!!! TTTTTAGGGAAGAAATTTTA+TGG + chr8_2:8461453-8461472 None:intergenic 20.0%
!!! GGACCATTTTTTTTTTTTTT+AGG + chr8_2:8461468-8461487 None:intergenic 20.0%
!! AACAGCATATTATATTGAAA+AGG + chr8_2:8461520-8461539 None:intergenic 20.0%
!!! GCTGTAATAAAATTTTAAGT+TGG - chr8_2:8461599-8461618 Msa1232190:intron 20.0%
!! TTATATAAGGACATAAATGA+TGG + chr8_2:8461652-8461671 None:intergenic 20.0%
!! AATAACAATCACTATCAATT+AGG - chr8_2:8461670-8461689 Msa1232190:intron 20.0%
!! GATAGTAAATAGTTAAAGAT+AGG + chr8_2:8461698-8461717 None:intergenic 20.0%
!! CTATAAGAAGAAAAAAGAAA+AGG + chr8_2:8461891-8461910 None:intergenic 20.0%
!!! TTTTCATATGGAAATTATGA+TGG - chr8_2:8462235-8462254 Msa1232190:intron 20.0%
!!! ATAATAAAGCATTGTAATCA+AGG + chr8_2:8462571-8462590 None:intergenic 20.0%
! GAAATTATATATATAGGGAC+AGG + chr8_2:8459675-8459694 None:intergenic 25.0%
! ACGTCAAAAAAAAAAAAAGG+CGG + chr8_2:8459712-8459731 None:intergenic 25.0%
! TATATATATACAAGGACAAG+TGG + chr8_2:8459748-8459767 None:intergenic 25.0%
! AACATAAAGATGACTATCTT+GGG + chr8_2:8459935-8459954 None:intergenic 25.0%
! TAACATAAAGATGACTATCT+TGG + chr8_2:8459936-8459955 None:intergenic 25.0%
! TCATCAATAAATATTGTACC+TGG + chr8_2:8460064-8460083 None:intergenic 25.0%
! ACACTACAATCATAATGTTT+AGG - chr8_2:8460131-8460150 Msa1232190:CDS 25.0%
!! AAAAACTTAGTTTGGAAACT+AGG - chr8_2:8460360-8460379 Msa1232190:CDS 25.0%
!! GTCAAAAATGTCTTCTTTTA+TGG + chr8_2:8460637-8460656 None:intergenic 25.0%
!! GTCAAACAATGTTTTCTTTA+AGG + chr8_2:8460779-8460798 None:intergenic 25.0%
!! GAAAAAAATCTGAAACAAAC+AGG + chr8_2:8461001-8461020 None:intergenic 25.0%
!!! CCTAATAGCTTTACATTTTA+GGG + chr8_2:8461127-8461146 None:intergenic 25.0%
!!! TCCTAATAGCTTTACATTTT+AGG + chr8_2:8461128-8461147 None:intergenic 25.0%
! ATGACAATCAGCTAAATTAA+GGG + chr8_2:8461351-8461370 None:intergenic 25.0%
! AATGACAATCAGCTAAATTA+AGG + chr8_2:8461352-8461371 None:intergenic 25.0%
! TAATTTAGCTGATTGTCATT+CGG - chr8_2:8461352-8461371 Msa1232190:intron 25.0%
!! ACAAAGACAGAGAATTTTTA+TGG + chr8_2:8461489-8461508 None:intergenic 25.0%
! ATAAAAATTCTCTGTCTTTG+TGG - chr8_2:8461488-8461507 Msa1232190:intron 25.0%
! TAAAAATTCTCTGTCTTTGT+GGG - chr8_2:8461489-8461508 Msa1232190:intron 25.0%
!!! TCTCTAACATGTTTTTAGAT+TGG - chr8_2:8461722-8461741 Msa1232190:intron 25.0%
! AAAGTCAAGAACTAATTTAG+TGG - chr8_2:8461808-8461827 Msa1232190:intron 25.0%
!!! CAATCTTTTGATTTGTATCT+TGG - chr8_2:8462271-8462290 Msa1232190:intron 25.0%
!!! TATCTGTTGATTCTGATAAA+TGG + chr8_2:8462458-8462477 None:intergenic 25.0%
! TTATAAGGTTAATGTCTTGT+TGG - chr8_2:8462647-8462666 Msa1232190:five_prime_UTR 25.0%
!!! TTTTTAAAGCTTGTTATGAG+AGG - chr8_2:8462715-8462734 Msa1232190:five_prime_UTR 25.0%
ATATAATTTCCAGTAAGGGA+CGG - chr8_2:8459685-8459704 Msa1232190:three_prime_UTR 30.0%
!! CAGCTTTTAGAGATATACAT+TGG + chr8_2:8459771-8459790 None:intergenic 30.0%
GCTTGACTATTGCAAATAAA+TGG + chr8_2:8459986-8460005 None:intergenic 30.0%
! TTTGTCTAGTTGTTCTGAAT+TGG + chr8_2:8460008-8460027 None:intergenic 30.0%
GCTTACTTGATTATGTTATG+TGG + chr8_2:8460093-8460112 None:intergenic 30.0%
AAGATGAGAAGAAAACTTTG+AGG - chr8_2:8460387-8460406 Msa1232190:CDS 30.0%
TCACTTAAACACCTTGAAAA+AGG + chr8_2:8460503-8460522 None:intergenic 30.0%
TTGGTTAAGGATCCTAAAAA+CGG + chr8_2:8460711-8460730 None:intergenic 30.0%
! TGATTACAACCGTTTAACTT+AGG - chr8_2:8460975-8460994 Msa1232190:intron 30.0%
CCCTAAAATGTAAAGCTATT+AGG - chr8_2:8461124-8461143 Msa1232190:intron 30.0%
AACTGATAAGTGGCTAAATT+TGG + chr8_2:8461628-8461647 None:intergenic 30.0%
AAATGATGGTAACTGATAAG+TGG + chr8_2:8461638-8461657 None:intergenic 30.0%
TACATACTCTCATAAGAACT+TGG - chr8_2:8461928-8461947 Msa1232190:intron 30.0%
!!! GGTTTTATAAGCATTGATTG+TGG - chr8_2:8461986-8462005 Msa1232190:intron 30.0%
GATTACTTTCAAGATGATAC+CGG - chr8_2:8462019-8462038 Msa1232190:intron 30.0%
! GTCTGTTTTATACCAAATTC+CGG + chr8_2:8462041-8462060 None:intergenic 30.0%
ATTTGGTATAAAACAGACGA+AGG - chr8_2:8462043-8462062 Msa1232190:intron 30.0%
TTTGGTATAAAACAGACGAA+GGG - chr8_2:8462044-8462063 Msa1232190:intron 30.0%
TACACATTGAAACCAAAAGA+AGG - chr8_2:8462181-8462200 Msa1232190:intron 30.0%
! ATTCGTGCTTTCTTTTCATA+TGG - chr8_2:8462223-8462242 Msa1232190:intron 30.0%
ACATTTATAGTGTCAGTTGA+AGG + chr8_2:8462367-8462386 None:intergenic 30.0%
AATTCCTTGCATTTCTTCAT+TGG - chr8_2:8462411-8462430 Msa1232190:CDS 30.0%
AATTCCAATGAAGAAATGCA+AGG + chr8_2:8462418-8462437 None:intergenic 30.0%
ATGTCAAAGTGTAATGAGTA+GGG - chr8_2:8462598-8462617 Msa1232190:five_prime_UTR 30.0%
ATTGCAAATAAATGGCACCA+AGG + chr8_2:8459978-8459997 None:intergenic 35.0%
ATTGCTAACAACGAAAATCC+AGG - chr8_2:8460043-8460062 Msa1232190:CDS 35.0%
CGCTCTGTAAAAACTTAGTT+TGG - chr8_2:8460352-8460371 Msa1232190:CDS 35.0%
! ATGAGAAGAAAACTTTGAGG+AGG - chr8_2:8460390-8460409 Msa1232190:CDS 35.0%
!! GCTAGAATTCACCTTTTTCA+AGG - chr8_2:8460489-8460508 Msa1232190:intron 35.0%
! TTTCAAGGTGTTTAAGTGAG+AGG - chr8_2:8460504-8460523 Msa1232190:intron 35.0%
ATAATATGGGTCATGCTAAC+CGG - chr8_2:8460567-8460586 Msa1232190:intron 35.0%
!!! TCCTTTTTTGGCAACCTTAA+CGG + chr8_2:8460606-8460625 None:intergenic 35.0%
TATTGGTGTATCTCAAGCAA+AGG - chr8_2:8460919-8460938 Msa1232190:intron 35.0%
ACAAACAGGCCTAAGTTAAA+CGG + chr8_2:8460987-8461006 None:intergenic 35.0%
ATTGTCATTCGGAAAGAGAA+AGG - chr8_2:8461363-8461382 Msa1232190:intron 35.0%
AAACTATCGTGCAAAAGTCA+AGG + chr8_2:8461411-8461430 None:intergenic 35.0%
TTCAAGATGATACCGGAATT+TGG - chr8_2:8462026-8462045 Msa1232190:intron 35.0%
!! AGGATAGTTTAGGTTGATAG+TGG + chr8_2:8462104-8462123 None:intergenic 35.0%
AACCTAAACTATCCTTACGT+TGG - chr8_2:8462109-8462128 Msa1232190:intron 35.0%
ACCTAAACTATCCTTACGTT+GGG - chr8_2:8462110-8462129 Msa1232190:intron 35.0%
GTTAATTGTTTCCCAACGTA+AGG + chr8_2:8462124-8462143 None:intergenic 35.0%
! TGTATCTTGGAGTTGATCTT+TGG - chr8_2:8462284-8462303 Msa1232190:intron 35.0%
GAAGAAATGCAAGGAATTGT+TGG + chr8_2:8462409-8462428 None:intergenic 35.0%
CTAACATCCATTTCCAACAT+TGG + chr8_2:8462502-8462521 None:intergenic 35.0%
CAATGTTGGAAATGGATGTT+AGG - chr8_2:8462500-8462519 Msa1232190:five_prime_UTR 35.0%
GATGATTACTTGGTTGAACA+GGG + chr8_2:8462544-8462563 None:intergenic 35.0%
TGATGATTACTTGGTTGAAC+AGG + chr8_2:8462545-8462564 None:intergenic 35.0%
!! TCAAGGTGTTGATGATTACT+TGG + chr8_2:8462554-8462573 None:intergenic 35.0%
CATGTCAAAGTGTAATGAGT+AGG - chr8_2:8462597-8462616 Msa1232190:five_prime_UTR 35.0%
TGTCAAAGTGTAATGAGTAG+GGG - chr8_2:8462599-8462618 Msa1232190:five_prime_UTR 35.0%
GAGTCGAGTTAGGATTTATA+AGG - chr8_2:8462632-8462651 Msa1232190:five_prime_UTR 35.0%
ATATATAGGGACAGGTCACA+GGG + chr8_2:8459667-8459686 None:intergenic 40.0%
TATATATAGGGACAGGTCAC+AGG + chr8_2:8459668-8459687 None:intergenic 40.0%
CTGTTTGTATTCGCAGAACT+TGG - chr8_2:8459788-8459807 Msa1232190:three_prime_UTR 40.0%
TCGTGAGTTCGTATATGTCA+TGG - chr8_2:8459834-8459853 Msa1232190:three_prime_UTR 40.0%
TTCCTTGCAGACAGATTACA+TGG - chr8_2:8459898-8459917 Msa1232190:CDS 40.0%
TTCCATGTAATCTGTCTGCA+AGG + chr8_2:8459903-8459922 None:intergenic 40.0%
TGAGCATAGTTCAGTTAGCA+TGG + chr8_2:8460533-8460552 None:intergenic 40.0%
!!! CCTTTTTTGGCAACCTTAAC+GGG + chr8_2:8460605-8460624 None:intergenic 40.0%
TAGGATCCTTAACCAATGTC+CGG - chr8_2:8460715-8460734 Msa1232190:intron 40.0%
TAAAACAGACGAAGGGTTTG+TGG - chr8_2:8462051-8462070 Msa1232190:intron 40.0%
TCCCAACGTAAGGATAGTTT+AGG + chr8_2:8462114-8462133 None:intergenic 40.0%
ACATTGAGATGTTTCCCAGA+AGG - chr8_2:8462145-8462164 Msa1232190:intron 40.0%
AGCAGTTTCTATCTCCTTCT+GGG + chr8_2:8462162-8462181 None:intergenic 40.0%
TAGCAGTTTCTATCTCCTTC+TGG + chr8_2:8462163-8462182 None:intergenic 40.0%
! CAACATTGGTTGTGGATCAT+TGG + chr8_2:8462488-8462507 None:intergenic 40.0%
CAATGATCCACAACCAATGT+TGG - chr8_2:8462486-8462505 Msa1232190:exon 40.0%
TCCACAACCAATGTTGGAAA+TGG - chr8_2:8462492-8462511 Msa1232190:exon 40.0%
! TCCATTTCCAACATTGGTTG+TGG + chr8_2:8462496-8462515 None:intergenic 40.0%
ATGGATGTTAGGTTCGATGT+TGG - chr8_2:8462511-8462530 Msa1232190:five_prime_UTR 40.0%
AAAGTGTAATGAGTAGGGGT+GGG - chr8_2:8462603-8462622 Msa1232190:five_prime_UTR 40.0%
CCCGTTAAGGTTGCCAAAAA+AGG - chr8_2:8460602-8460621 Msa1232190:intron 45.0%
AGGATCCTTAACCAATGTCC+GGG - chr8_2:8460716-8460735 Msa1232190:intron 45.0%
AATGTGTGCCTTGTGAAGAC+TGG - chr8_2:8462382-8462401 Msa1232190:CDS 45.0%
TTGTTGGACCAGTCTTCACA+AGG + chr8_2:8462393-8462412 None:intergenic 45.0%
CAAAGTGTAATGAGTAGGGG+TGG - chr8_2:8462602-8462621 Msa1232190:five_prime_UTR 45.0%
! TGGGAATTTCGAGTCGAGTT+AGG - chr8_2:8462622-8462641 Msa1232190:five_prime_UTR 45.0%
!! AAAAAAAAAAAAAAAAAAAC+AGG - chr8_2:8461541-8461560 Msa1232190:intron 5.0%
GGATCCTTAACCAATGTCCG+GGG - chr8_2:8460717-8460736 Msa1232190:intron 50.0%
GATCCTTAACCAATGTCCGG+GGG - chr8_2:8460718-8460737 Msa1232190:intron 50.0%
GACGAAGGGTTTGTGGAAAC+AGG - chr8_2:8462058-8462077 Msa1232190:intron 50.0%
TACTTGGTTGAACAGGGCGA+GGG + chr8_2:8462538-8462557 None:intergenic 50.0%
TTACTTGGTTGAACAGGGCG+AGG + chr8_2:8462539-8462558 None:intergenic 50.0%
! ATGCCCCCGGACATTGGTTA+AGG + chr8_2:8460724-8460743 None:intergenic 55.0%
GAAATGCTAGTCGATGCCCC+CGG + chr8_2:8460737-8460756 None:intergenic 55.0%
ACTTGGTTGAACAGGGCGAG+GGG + chr8_2:8462537-8462556 None:intergenic 55.0%
AAAGGCGGACCGTCCCTTAC+TGG + chr8_2:8459697-8459716 None:intergenic 60.0%
AGCGTGCGTAGTGCTTGCCT+TGG - chr8_2:8459958-8459977 Msa1232190:CDS 60.0%
! TGGCAACCTTAACGGGTGCC+CGG + chr8_2:8460598-8460617 None:intergenic 60.0%
TAGTCGATGCCCCCGGACAT+TGG + chr8_2:8460730-8460749 None:intergenic 60.0%
GTCATGCTAACCGGTGCCCC+CGG - chr8_2:8460576-8460595 Msa1232190:intron 65.0%
TCATGCTAACCGGTGCCCCC+GGG - chr8_2:8460577-8460596 Msa1232190:intron 65.0%
! CAACCTTAACGGGTGCCCGG+GGG + chr8_2:8460595-8460614 None:intergenic 65.0%
! GCAACCTTAACGGGTGCCCG+GGG + chr8_2:8460596-8460615 None:intergenic 65.0%
! GGCAACCTTAACGGGTGCCC+GGG + chr8_2:8460597-8460616 None:intergenic 65.0%
! TAACGGGTGCCCGGGGGCAC+CGG + chr8_2:8460589-8460608 None:intergenic 75.0%
GTGCCCCCGGGCACCCGTTA+AGG - chr8_2:8460589-8460608 Msa1232190:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 8459647 8462765 8459647 ID=Msa1232190;Name=Msa1232190
chr8_2 mRNA 8459647 8462765 8459647 ID=Msa1232190-mRNA-1;Parent=Msa1232190;Name=Msa1232190-mRNA-1;_AED=0.28;_eAED=0.28;_QI=270|1|1|1|1|1|2|207|239
chr8_2 exon 8459647 8460425 8459647 ID=Msa1232190-mRNA-1:exon:3326;Parent=Msa1232190-mRNA-1
chr8_2 exon 8462348 8462765 8462348 ID=Msa1232190-mRNA-1:exon:3325;Parent=Msa1232190-mRNA-1
chr8_2 five_prime_UTR 8462496 8462765 8462496 ID=Msa1232190-mRNA-1:five_prime_utr;Parent=Msa1232190-mRNA-1
chr8_2 CDS 8462348 8462495 8462348 ID=Msa1232190-mRNA-1:cds;Parent=Msa1232190-mRNA-1
chr8_2 CDS 8459854 8460425 8459854 ID=Msa1232190-mRNA-1:cds;Parent=Msa1232190-mRNA-1
chr8_2 three_prime_UTR 8459647 8459853 8459647 ID=Msa1232190-mRNA-1:three_prime_utr;Parent=Msa1232190-mRNA-1
Gene Sequence

>Msa1232190

ATGGAAAGAAAACTACCCAAGATAGTCATCTTTATGTTAAAAGCGTGCGTAGTGCTTGCCTTGGTGCCATTTATTTGCAATAGTCAAGCCAATTCAGAACAACTAGACAAATCTGTTTCAAGAGAAATTGCTAACAACGAAAATCCAGGTTTTATAAGCATTGATTGTGGATCAAAAGTTGATTACTTTCAAGATGATACCGGAATTTGGTATAAAACAGACGAAGGGTTTGTGGAAACAGGAGAGAATCATGTCACTTCATCCACTATCAACCTAAACTATCCTTACGTTGGGAAACAATTAACAACATTGAGATGTTTCCCAGAAGGAGATAGAAACTGCTACACATTGAAACCAAAAGAAGGAAAAAATAAAAAGTATTTGATTCGTGCTTTCTTTTCATATGGAAATTATGATGGAAAAAATCAAACTCAATCTTTTGATTTGTATCTTGGAGTTGATCTTTGGAAAAAAATCAATTTCACTAATACTATTTATTACTCTTATACGGAGATCATTCACACTCCTTCAACTGACACTATAAATGTGTGCCTTGTGAAGACTGGTCCAACAATTCCTTGCATTTCTTCATTGGAATTGAGACTTTTAAACAACTCCATTTATCAGAATCAACAGATAATCTCTGCCAATGATCCACAACCAATGTTGGAAATGGATGTTAGGTTCGATGTTGGCCCCTCGCCCTGTTCAACCAAGTAA
Protein sequence

>Msa1232190

MERKLPKIVIFMLKACVVLALVPFICNSQANSEQLDKSVSREIANNENPGFISIDCGSKVDYFQDDTGIWYKTDEGFVETGENHVTSSTINLNYPYVGKQLTTLRCFPEGDRNCYTLKPKEGKNKKYLIRAFFSYGNYDGKNQTQSFDLYLGVDLWKKINFTNTIYYSYTEIIHTPSTDTINVCLVKTGPTIPCISSLELRLLNNSIYQNQQIISANDPQPMLEMDVRFDVGPSPCSTK*