AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1234640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234640 MtrunA17_Chr8g0344921 98.319 119 2 0 1 119 1 119 5.59e-87 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234640 AT3G59650.1 82.353 119 21 0 1 119 1 119 2.03e-73 214
Msa1234640 AT3G59650.2 67.123 146 21 1 1 119 1 146 1.55e-67 200

Find 69 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTTGATAGGGCATTTA+TGG 0.120191 8_2:-12178217 Msa1234640:intron
GTCGCATTTGCCAGCGTTTA+AGG 0.191698 8_2:-12178193 Msa1234640:CDS
CACACACCACTCGGTCATTA+TGG 0.253183 8_2:+12178011 None:intergenic
GTGAGCTGTGCGGCGGCGTT+AGG 0.286266 8_2:+12180367 None:intergenic
GTAACTATGAATTATTTCTT+TGG 0.303962 8_2:+12177522 None:intergenic
GACAACTGCTTTGAAATATT+AGG 0.339185 8_2:-12177864 Msa1234640:exon
AGATGGCATTGCGAGGAGTT+TGG 0.367673 8_2:-12180294 Msa1234640:CDS
CGTTGTTAGCTATAGCGATT+GGG 0.368754 8_2:-12180257 Msa1234640:CDS
TCCCAGTGCATTCCTTAGCC+TGG 0.373420 8_2:+12177949 None:intergenic
AATTCATAGTTACTATATAA+TGG 0.374144 8_2:-12177512 Msa1234640:three_prime_UTR
AGTGGTGTGTGTGAAGAATA+TGG 0.393785 8_2:-12177999 Msa1234640:CDS
AAAAGGTAGAAACTATATAA+TGG 0.394205 8_2:-12177471 Msa1234640:three_prime_UTR
ACCTTGCACGCTAGGGTGTT+TGG 0.399445 8_2:+12177892 None:intergenic
TGAGCTGTGCGGCGGCGTTA+GGG 0.416592 8_2:+12180368 None:intergenic
TGTTGTTTGGCGCTATTGCC+AGG 0.418369 8_2:-12177747 Msa1234640:three_prime_UTR
GCTTAAGTTCTCATGTTGTT+TGG 0.421272 8_2:-12177760 Msa1234640:three_prime_UTR
CTGCTGACGGTGATCGGAGA+CGG 0.432105 8_2:+12180337 None:intergenic
TTAATCAAATATCTGTGAAC+TGG 0.439984 8_2:-12177786 Msa1234640:three_prime_UTR
GTGGAAGTAGCAAAGGCATT+AGG 0.442216 8_2:-12180234 Msa1234640:intron
ATCTGATGAACGGCGGTTGT+TGG 0.442728 8_2:+12180313 None:intergenic
GTCCATGTACCTTGCACGCT+AGG 0.449503 8_2:+12177884 None:intergenic
TGCTGACGGTGATCGGAGAC+GGG 0.450868 8_2:+12180338 None:intergenic
GCATGCAGAAGTATCTCTTC+CGG 0.466200 8_2:+12177974 None:intergenic
GGAGGCGAACCCTCAGTTAG+AGG 0.466422 8_2:-12178172 Msa1234640:CDS
GTATGTATTAGTCAACAAAA+AGG 0.478181 8_2:-12177488 Msa1234640:three_prime_UTR
TGATTAACTGACCAGAGAAA+AGG 0.500162 8_2:+12177802 None:intergenic
ACCCTAGCGTGCAAGGTACA+TGG 0.511271 8_2:-12177886 Msa1234640:CDS
TCGTTGTTAGCTATAGCGAT+TGG 0.511918 8_2:-12180258 Msa1234640:CDS
TGGTCAGCATCCACATCTAA+AGG 0.514097 8_2:-12178130 Msa1234640:CDS
TTCTGCATGCAACCAGGCTA+AGG 0.518615 8_2:-12177961 Msa1234640:CDS
AACCAGGCTAAGGAATGCAC+TGG 0.528014 8_2:-12177951 Msa1234640:CDS
TACAAACTAACAATATATAA+CGG 0.542968 8_2:+12177635 None:intergenic
AACAACCGCCGTTCATCAGA+TGG 0.544181 8_2:-12180311 Msa1234640:exon
ATGGTGTTTAACTGAAATCC+TGG 0.546587 8_2:+12177729 None:intergenic
GGGTTCGCCTCCTTAAACGC+TGG 0.551134 8_2:+12178183 None:intergenic
TCCATGTACCTTGCACGCTA+GGG 0.551964 8_2:+12177885 None:intergenic
GATTAACTATCAGCTGATTG+TGG 0.553497 8_2:-12177681 Msa1234640:three_prime_UTR
ACCAAACACCCTAGCGTGCA+AGG 0.562815 8_2:-12177893 Msa1234640:CDS
GTTGGACTGCTGACGGTGAT+CGG 0.564998 8_2:+12180331 None:intergenic
TCAGTGTCCACCTCTAACTG+AGG 0.586148 8_2:+12178162 None:intergenic
ACACTTAAAAGACTAAGCTA+TGG 0.587092 8_2:+12177710 None:intergenic
GTGTGTGAAGAATATGGATC+CGG 0.590195 8_2:-12177993 Msa1234640:CDS
GGCTAAGGAATGCACTGGGA+AGG 0.590269 8_2:-12177946 Msa1234640:CDS
GTGGACACTGAAATGATTCG+TGG 0.592382 8_2:-12178150 Msa1234640:CDS
GCTGACGGTGATCGGAGACG+GGG 0.594296 8_2:+12180339 None:intergenic
AGATACTTCTGCATGCAACC+AGG 0.597112 8_2:-12177967 Msa1234640:CDS
CAATTGGAGAAAACTGACGT+GGG 0.605757 8_2:+12180427 None:intergenic
CAGTGTCCACCTCTAACTGA+GGG 0.607705 8_2:+12178163 None:intergenic
GGATCGACTTAGCACTACTA+CGG 0.608740 8_2:+12177823 None:intergenic
GGCGGTTGTTGGACTGCTGA+CGG 0.611773 8_2:+12180324 None:intergenic
TATTCTTCACACACACCACT+CGG 0.614073 8_2:+12178002 None:intergenic
GATTGGGGTGGAAGTAGCAA+AGG 0.624222 8_2:-12180241 Msa1234640:CDS
GGCGAACCCTCAGTTAGAGG+TGG 0.624239 8_2:-12178169 Msa1234640:CDS
TCAATTGGAGAAAACTGACG+TGG 0.630988 8_2:+12180426 None:intergenic
TTTCTTTGGCGAGTAAACCA+TGG 0.636878 8_2:+12177536 None:intergenic
TTATCTTCGCTAGAGAACCA+TGG 0.637249 8_2:-12177553 Msa1234640:three_prime_UTR
GTTGTTAGCTATAGCGATTG+GGG 0.643269 8_2:-12180256 Msa1234640:CDS
GCATTTGCCAGCGTTTAAGG+AGG 0.645640 8_2:-12178190 Msa1234640:CDS
GAAGGAAAGTAATTAAGCTG+AGG 0.646557 8_2:-12177928 Msa1234640:CDS
ACCAGGCTAAGGAATGCACT+GGG 0.650150 8_2:-12177950 Msa1234640:CDS
GGGCTCTGTGAGCTGTGCGG+CGG 0.653718 8_2:+12180360 None:intergenic
TATAACGGAGTAAACAAACT+CGG 0.668327 8_2:+12177650 None:intergenic
GTTAGCTATAGCGATTGGGG+TGG 0.672048 8_2:-12180253 Msa1234640:CDS
TCGCAATGCCATCTGATGAA+CGG 0.675132 8_2:+12180303 None:intergenic
GTTCATCAGATGGCATTGCG+AGG 0.675557 8_2:-12180301 Msa1234640:exon
CTGACGGTGATCGGAGACGG+GGG 0.677896 8_2:+12180340 None:intergenic
AATTGGAGAAAACTGACGTG+GGG 0.687824 8_2:+12180428 None:intergenic
CAATGCCATCTGATGAACGG+CGG 0.721466 8_2:+12180306 None:intergenic
AGAGAACCATAATGACCGAG+TGG 0.735330 8_2:-12178017 Msa1234640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTATAATTTTATTCATTTA+CGG + chr8_2:12177475-12177494 None:intergenic 10.0%
!!! TTATTTTTAATTAAAAGAGT+TGG - chr8_2:12177519-12177538 Msa1234640:three_prime_UTR 10.0%
!!! TATTTTTAATTAAAAGAGTT+GGG - chr8_2:12177520-12177539 Msa1234640:three_prime_UTR 10.0%
!! ATAAAAAAATGAAAACTTTA+GGG + chr8_2:12177842-12177861 None:intergenic 10.0%
!! AATAAAAAAATGAAAACTTT+AGG + chr8_2:12177843-12177862 None:intergenic 10.0%
!!! AAAGTTTTCATTTTTTTATT+TGG - chr8_2:12177843-12177862 Msa1234640:three_prime_UTR 10.0%
!!! AAGTTTTCATTTTTTTATTT+GGG - chr8_2:12177844-12177863 Msa1234640:three_prime_UTR 10.0%
!!! TTTTTAAAAAACTGTTTTTA+TGG - chr8_2:12178666-12178685 Msa1234640:intron 10.0%
!!! TTTTTTTTAAACTGTTTTTA+TGG - chr8_2:12179461-12179480 Msa1234640:intron 10.0%
!!! TTTAGTTTTAAATATTTGAT+AGG - chr8_2:12179752-12179771 Msa1234640:intron 10.0%
!!! TTAGTTTTAAATATTTGATA+GGG - chr8_2:12178950-12178969 Msa1234640:intron 10.0%
!!! ATTTTTTTATTTGGGTATTT+AGG - chr8_2:12177852-12177871 Msa1234640:exon 15.0%
!! AAATCAAACAGATATAATAT+TGG - chr8_2:12178350-12178369 Msa1234640:intron 15.0%
!!! TTTTTTAAAAATTTCTGCAT+CGG + chr8_2:12178657-12178676 None:intergenic 15.0%
!!! CTTAGTTTTAAATATTTGAT+AGG - chr8_2:12178949-12178968 Msa1234640:intron 15.0%
!!! AAATGTTTAGAATGTTATTT+TGG + chr8_2:12179269-12179288 None:intergenic 15.0%
!! TACAAACTAACAATATATAA+CGG + chr8_2:12180347-12180366 None:intergenic 15.0%
!! GATAAAAAAAAAAAGTTCAA+AGG + chr8_2:12180411-12180430 None:intergenic 15.0%
!! AATTCATAGTTACTATATAA+TGG - chr8_2:12180467-12180486 Msa1234640:five_prime_UTR 15.0%
!!! GATTTTGAGAAATTTTCAAT+TGG + chr8_2:12177571-12177590 None:intergenic 20.0%
!!! CTTATATGGTTATTTTTGTA+AGG - chr8_2:12178167-12178186 Msa1234640:CDS 20.0%
!! GTTTATAATTCTAAATGTCT+AGG - chr8_2:12178223-12178242 Msa1234640:intron 20.0%
!!! TAATATTGGTGATATTTTCT+TGG - chr8_2:12178438-12178457 Msa1234640:intron 20.0%
!!! GAAATCATTTAAAATCTGAA+TGG - chr8_2:12178542-12178561 Msa1234640:intron 20.0%
!!! GTAACTATGAATTATTTCTT+TGG + chr8_2:12180460-12180479 None:intergenic 20.0%
! GAAAACTGAAAGAATGTAAA+GGG + chr8_2:12177786-12177805 None:intergenic 25.0%
! TGAAAACTGAAAGAATGTAA+AGG + chr8_2:12177787-12177806 None:intergenic 25.0%
!!! TTAGGTTTGTTGAATTATTG+TGG - chr8_2:12177870-12177889 Msa1234640:CDS 25.0%
!!! TCTTGCTTTTATTCAAATAG+TGG - chr8_2:12177909-12177928 Msa1234640:CDS 25.0%
!! AGATTGATTACAGTCTTATA+TGG - chr8_2:12178153-12178172 Msa1234640:CDS 25.0%
! TTAAAAGGTCTGAAAAGAAT+AGG + chr8_2:12178298-12178317 None:intergenic 25.0%
!!! AAAATAGTTTCTTTTTTCCC+TGG - chr8_2:12178513-12178532 Msa1234640:intron 25.0%
! TAAATGATTTCAAAACTCCA+GGG + chr8_2:12178533-12178552 None:intergenic 25.0%
! TTAAATGATTTCAAAACTCC+AGG + chr8_2:12178534-12178553 None:intergenic 25.0%
!!! AAAACTGTTTTTATGGTTTG+AGG - chr8_2:12178673-12178692 Msa1234640:intron 25.0%
! AATATTTGATAGGCCATTTA+TGG - chr8_2:12178959-12178978 Msa1234640:intron 25.0%
!!! AAAATTGTTGCTTTTTTCCT+TGG - chr8_2:12179200-12179219 Msa1234640:intron 25.0%
! AAAATAGTATGCATCAGATT+CGG + chr8_2:12179438-12179457 None:intergenic 25.0%
! ATTGTTAAAAGGTTGTGATT+TGG + chr8_2:12179568-12179587 None:intergenic 25.0%
! TCTCGAATTTGATTGTTAAA+AGG + chr8_2:12179579-12179598 None:intergenic 25.0%
!! TGAATTTTACCTAGTACTAA+AGG - chr8_2:12179655-12179674 Msa1234640:intron 25.0%
!! AATATTTGATAGGGCATTTA+TGG - chr8_2:12179762-12179781 Msa1234640:intron 25.0%
!! CTTTTTACAGTAAGATTCTT+GGG - chr8_2:12179872-12179891 Msa1234640:intron 25.0%
! TTAATCAAATATCTGTGAAC+TGG - chr8_2:12180193-12180212 Msa1234640:intron 25.0%
! GTATGTATTAGTCAACAAAA+AGG - chr8_2:12180491-12180510 Msa1234640:five_prime_UTR 25.0%
TTGAACAAATCGCTATTGTT+CGG + chr8_2:12178081-12178100 None:intergenic 30.0%
! GTAATTCGAGTTTATTCTCA+CGG + chr8_2:12178196-12178215 None:intergenic 30.0%
TCTAGGTGATATGATAGTAT+AGG - chr8_2:12178240-12178259 Msa1234640:intron 30.0%
AAAAATTTCTGCATCGGATT+CGG + chr8_2:12178651-12178670 None:intergenic 30.0%
TTTGAGGACAATGCTAATAA+TGG - chr8_2:12178689-12178708 Msa1234640:intron 30.0%
GCTAATAATGGCATTGTAAA+TGG - chr8_2:12178701-12178720 Msa1234640:intron 30.0%
TCTCAGACTTGATTGTTAAA+AGG + chr8_2:12178783-12178802 None:intergenic 30.0%
! TGCATTTTACCTAGTACTAA+AGG - chr8_2:12178859-12178878 Msa1234640:intron 30.0%
TATTCATAGCATATTGTTCC+AGG + chr8_2:12178885-12178904 None:intergenic 30.0%
!! TATTCTCTCCTGTTTTGAAT+AGG - chr8_2:12178915-12178934 Msa1234640:intron 30.0%
AACAATCTCCTATTCAAAAC+AGG + chr8_2:12178926-12178945 None:intergenic 30.0%
!! TTTTTCAATGATTGACTCCT+AGG + chr8_2:12179017-12179036 None:intergenic 30.0%
!!! TATTAGTGATTTTGGAGTCA+TGG - chr8_2:12179046-12179065 Msa1234640:intron 30.0%
!!! TGTTATTTTGGTTCTAGCAA+TGG + chr8_2:12179257-12179276 None:intergenic 30.0%
!! TCAGAGGCATTGAAAAAATA+TGG - chr8_2:12179355-12179374 Msa1234640:intron 30.0%
! TATTTTTCCTTTTGCCTGAT+TGG - chr8_2:12179523-12179542 Msa1234640:intron 30.0%
TAACAATCAAATTCGAGAGA+AGG - chr8_2:12179581-12179600 Msa1234640:intron 30.0%
! GCTTTTTACAGTAAGATTCT+TGG - chr8_2:12179871-12179890 Msa1234640:intron 30.0%
! TGTAATTGATGATTCTCTTG+TGG - chr8_2:12179934-12179953 Msa1234640:intron 30.0%
!! GACAACTGCTTTGAAATATT+AGG - chr8_2:12180115-12180134 Msa1234640:intron 30.0%
ACACTTAAAAGACTAAGCTA+TGG + chr8_2:12180272-12180291 None:intergenic 30.0%
! TATAACGGAGTAAACAAACT+CGG + chr8_2:12180332-12180351 None:intergenic 30.0%
ATCTCAATCATTATGTGCGT+GGG + chr8_2:12177947-12177966 None:intergenic 35.0%
AATCTCAATCATTATGTGCG+TGG + chr8_2:12177948-12177967 None:intergenic 35.0%
TTGATCAGTGTTACCAATAG+TGG - chr8_2:12177966-12177985 Msa1234640:CDS 35.0%
! TATTTTCCACGATCCACTAT+TGG + chr8_2:12177982-12178001 None:intergenic 35.0%
CAAATTTCAACTCGATTGCT+AGG - chr8_2:12178463-12178482 Msa1234640:intron 35.0%
TATTCTTCTTGTTGCCTGAT+TGG - chr8_2:12178728-12178747 Msa1234640:intron 35.0%
TAACAATCAAGTCTGAGAGA+AGG - chr8_2:12178785-12178804 Msa1234640:intron 35.0%
TTGCAAGCGTAATAAGGTTT+AGG - chr8_2:12178990-12179009 Msa1234640:intron 35.0%
TGCAAGCGTAATAAGGTTTA+GGG - chr8_2:12178991-12179010 Msa1234640:intron 35.0%
! CTAGCACGTATTAGTGATTT+TGG - chr8_2:12179038-12179057 Msa1234640:intron 35.0%
CTAAGAAGTCAGTAAAACCA+AGG + chr8_2:12179220-12179239 None:intergenic 35.0%
GCTAAGAATAACGTTGTAGA+TGG - chr8_2:12179496-12179515 Msa1234640:intron 35.0%
TCAGGCAAAAGGAAAAATAC+AGG + chr8_2:12179522-12179541 None:intergenic 35.0%
TTTACCTAGTACTAAAGGCT+CGG - chr8_2:12179660-12179679 Msa1234640:intron 35.0%
!! TATTCTCGCCTGTTTTGAAT+AGG - chr8_2:12179718-12179737 Msa1234640:intron 35.0%
!! ATTCTCGCCTGTTTTGAATA+GGG - chr8_2:12179719-12179738 Msa1234640:intron 35.0%
AAGAATCCCCTATTCAAAAC+AGG + chr8_2:12179729-12179748 None:intergenic 35.0%
CTGTAAAAAGCCTTTAGATG+TGG + chr8_2:12179862-12179881 None:intergenic 35.0%
GAAGGAAAGTAATTAAGCTG+AGG - chr8_2:12180051-12180070 Msa1234640:intron 35.0%
TGATTAACTGACCAGAGAAA+AGG + chr8_2:12180180-12180199 None:intergenic 35.0%
GCTTAAGTTCTCATGTTGTT+TGG - chr8_2:12180219-12180238 Msa1234640:intron 35.0%
ATGGTGTTTAACTGAAATCC+TGG + chr8_2:12180253-12180272 None:intergenic 35.0%
GATTAACTATCAGCTGATTG+TGG - chr8_2:12180298-12180317 Msa1234640:exon 35.0%
AATTGGAGAAAACTGACGTG+GGG + chr8_2:12177554-12177573 None:intergenic 40.0%
CAATTGGAGAAAACTGACGT+GGG + chr8_2:12177555-12177574 None:intergenic 40.0%
TCAATTGGAGAAAACTGACG+TGG + chr8_2:12177556-12177575 None:intergenic 40.0%
TCGTTGTTAGCTATAGCGAT+TGG - chr8_2:12177721-12177740 Msa1234640:three_prime_UTR 40.0%
CGTTGTTAGCTATAGCGATT+GGG - chr8_2:12177722-12177741 Msa1234640:three_prime_UTR 40.0%
GTTGTTAGCTATAGCGATTG+GGG - chr8_2:12177723-12177742 Msa1234640:three_prime_UTR 40.0%
AATCATTATGTGCGTGGGTT+GGG + chr8_2:12177942-12177961 None:intergenic 40.0%
! TAGTGGATCGTGGAAAATAG+CGG - chr8_2:12177983-12178002 Msa1234640:CDS 40.0%
! GTGGAAAATAGCGGTTTGTT+TGG - chr8_2:12177992-12178011 Msa1234640:CDS 40.0%
TGCAATGCTATAGCTGTTAC+TGG - chr8_2:12178027-12178046 Msa1234640:intron 40.0%
! GTGTTGTTAAAAAGTGACGC+TGG + chr8_2:12178131-12178150 None:intergenic 40.0%
! TGTCAACGTCGGTGTTTAAA+AGG + chr8_2:12178313-12178332 None:intergenic 40.0%
ATTACTACCAGTGTCAACGT+CGG + chr8_2:12178324-12178343 None:intergenic 40.0%
! ATACCTTTGATCAGAAGTGC+CGG - chr8_2:12178377-12178396 Msa1234640:intron 40.0%
AATCAGAAGCTATGTAGCAC+CGG + chr8_2:12178399-12178418 None:intergenic 40.0%
AGTCAAAGCAACTGAAAGAC+GGG + chr8_2:12178569-12178588 None:intergenic 40.0%
CAGTCAAAGCAACTGAAAGA+CGG + chr8_2:12178570-12178589 None:intergenic 40.0%
! TTTCAGTTGCTTTGACTGAG+AGG - chr8_2:12178572-12178591 Msa1234640:intron 40.0%
! TTCAGTTGCTTTGACTGAGA+GGG - chr8_2:12178573-12178592 Msa1234640:intron 40.0%
TTTACCTAGTACTAAAGGCC+TGG - chr8_2:12178864-12178883 Msa1234640:intron 40.0%
TGCAAATGCGACTCCATAAA+TGG + chr8_2:12178975-12178994 None:intergenic 40.0%
TCGCATTTGCAAGCGTAATA+AGG - chr8_2:12178984-12179003 Msa1234640:intron 40.0%
AGTCAAGGCAACTGAAAGAT+GGG + chr8_2:12179334-12179353 None:intergenic 40.0%
TAGTCAAGGCAACTGAAAGA+TGG + chr8_2:12179335-12179354 None:intergenic 40.0%
TTCAATGCCTCTGATAGTCA+AGG + chr8_2:12179349-12179368 None:intergenic 40.0%
TTGCCACATCAGAATTGTTC+TGG + chr8_2:12179382-12179401 None:intergenic 40.0%
AGATTCGGAGTGTGATTACA+TGG + chr8_2:12179423-12179442 None:intergenic 40.0%
! GACAATCAGAAGTAGCTTTG+TGG + chr8_2:12179633-12179652 None:intergenic 40.0%
!! TTCTCGCCTGTTTTGAATAG+GGG - chr8_2:12179720-12179739 Msa1234640:intron 40.0%
TATTCTTCACACACACCACT+CGG + chr8_2:12179980-12179999 None:intergenic 40.0%
AGTGGTGTGTGTGAAGAATA+TGG - chr8_2:12179980-12179999 Msa1234640:intron 40.0%
GTGTGTGAAGAATATGGATC+CGG - chr8_2:12179986-12180005 Msa1234640:intron 40.0%
TTATCTTCGCTAGAGAACCA+TGG - chr8_2:12180426-12180445 Msa1234640:five_prime_UTR 40.0%
TTTCTTTGGCGAGTAAACCA+TGG + chr8_2:12180446-12180465 None:intergenic 40.0%
TCGCAATGCCATCTGATGAA+CGG + chr8_2:12177679-12177698 None:intergenic 45.0%
!! GTGGAAGTAGCAAAGGCATT+AGG - chr8_2:12177745-12177764 Msa1234640:three_prime_UTR 45.0%
CAATCATTATGTGCGTGGGT+TGG + chr8_2:12177943-12177962 None:intergenic 45.0%
!! GTGTTACCAATAGTGGATCG+TGG - chr8_2:12177973-12177992 Msa1234640:CDS 45.0%
TTAAACACCGACGTTGACAC+TGG - chr8_2:12178314-12178333 Msa1234640:intron 45.0%
! TCAGTTGCTTTGACTGAGAG+GGG - chr8_2:12178574-12178593 Msa1234640:intron 45.0%
GGTCAAGAACAATTCTGCTG+TGG - chr8_2:12178610-12178629 Msa1234640:intron 45.0%
GAACAATTCTGCTGTGGCAA+CGG - chr8_2:12178616-12178635 Msa1234640:intron 45.0%
TGTTCCAGGCCTTTAGTACT+AGG + chr8_2:12178871-12178890 None:intergenic 45.0%
! CGTAATAAGGTTTAGGGCCT+AGG - chr8_2:12178997-12179016 Msa1234640:intron 45.0%
GAAGCCACTTGAAACCTGAA+TGG - chr8_2:12179307-12179326 Msa1234640:intron 45.0%
ACTGAAAGATGGGTCCATTC+AGG + chr8_2:12179324-12179343 None:intergenic 45.0%
TCAGTTGCCTTGACTATCAG+AGG - chr8_2:12179339-12179358 Msa1234640:intron 45.0%
GGTCCAGAACAATTCTGATG+TGG - chr8_2:12179376-12179395 Msa1234640:intron 45.0%
ACATGAAGTCACAGCCAATC+AGG + chr8_2:12179540-12179559 None:intergenic 45.0%
ACTTCCGAGCCTTTAGTACT+AGG + chr8_2:12179667-12179686 None:intergenic 45.0%
AGTACTAAAGGCTCGGAAGT+TGG - chr8_2:12179667-12179686 Msa1234640:intron 45.0%
GTGGACACTGAAATGATTCG+TGG - chr8_2:12179829-12179848 Msa1234640:intron 45.0%
TGGTCAGCATCCACATCTAA+AGG - chr8_2:12179849-12179868 Msa1234640:intron 45.0%
AGAGAACCATAATGACCGAG+TGG - chr8_2:12179962-12179981 Msa1234640:intron 45.0%
!! GCATGCAGAAGTATCTCTTC+CGG + chr8_2:12180008-12180027 None:intergenic 45.0%
AGATACTTCTGCATGCAACC+AGG - chr8_2:12180012-12180031 Msa1234640:intron 45.0%
! GGATCGACTTAGCACTACTA+CGG + chr8_2:12180159-12180178 None:intergenic 45.0%
! GCTAAGTCGATCCTTTTCTC+TGG - chr8_2:12180166-12180185 Msa1234640:intron 45.0%
!!! AATTTTATGAATTATAATAT+TGG - chr8_2:12178424-12178443 Msa1234640:intron 5.0%
ATCTGATGAACGGCGGTTGT+TGG + chr8_2:12177669-12177688 None:intergenic 50.0%
AACAACCGCCGTTCATCAGA+TGG - chr8_2:12177668-12177687 Msa1234640:three_prime_UTR 50.0%
CAATGCCATCTGATGAACGG+CGG + chr8_2:12177676-12177695 None:intergenic 50.0%
GTTCATCAGATGGCATTGCG+AGG - chr8_2:12177678-12177697 Msa1234640:three_prime_UTR 50.0%
AGATGGCATTGCGAGGAGTT+TGG - chr8_2:12177685-12177704 Msa1234640:three_prime_UTR 50.0%
GTTAGCTATAGCGATTGGGG+TGG - chr8_2:12177726-12177745 Msa1234640:three_prime_UTR 50.0%
GATTGGGGTGGAAGTAGCAA+AGG - chr8_2:12177738-12177757 Msa1234640:three_prime_UTR 50.0%
!! GAGAGGGGCGTTGAAAGAAT+TGG - chr8_2:12178589-12178608 Msa1234640:intron 50.0%
ACCTGAAGTCACAGCCAATC+AGG + chr8_2:12178745-12178764 None:intergenic 50.0%
AACCACAACGCTCTTTCTGC+TGG - chr8_2:12178832-12178851 Msa1234640:intron 50.0%
!! AACCAGCAGAAAGAGCGTTG+TGG + chr8_2:12178837-12178856 None:intergenic 50.0%
! GGGTCCATTCAGGTTTCAAG+TGG + chr8_2:12179314-12179333 None:intergenic 50.0%
GTCACAGCCAATCAGGCAAA+AGG + chr8_2:12179533-12179552 None:intergenic 50.0%
GTCGCATTTGCCAGCGTTTA+AGG - chr8_2:12179786-12179805 Msa1234640:intron 50.0%
GCATTTGCCAGCGTTTAAGG+AGG - chr8_2:12179789-12179808 Msa1234640:intron 50.0%
CAGTGTCCACCTCTAACTGA+GGG + chr8_2:12179819-12179838 None:intergenic 50.0%
TCAGTGTCCACCTCTAACTG+AGG + chr8_2:12179820-12179839 None:intergenic 50.0%
CACACACCACTCGGTCATTA+TGG + chr8_2:12179971-12179990 None:intergenic 50.0%
TTCTGCATGCAACCAGGCTA+AGG - chr8_2:12180018-12180037 Msa1234640:intron 50.0%
AACCAGGCTAAGGAATGCAC+TGG - chr8_2:12180028-12180047 Msa1234640:intron 50.0%
ACCAGGCTAAGGAATGCACT+GGG - chr8_2:12180029-12180048 Msa1234640:intron 50.0%
TCCATGTACCTTGCACGCTA+GGG + chr8_2:12180097-12180116 None:intergenic 50.0%
! TGTTGTTTGGCGCTATTGCC+AGG - chr8_2:12180232-12180251 Msa1234640:intron 50.0%
GTTGGACTGCTGACGGTGAT+CGG + chr8_2:12177651-12177670 None:intergenic 55.0%
! GCACCGGCACTTCTGATCAA+AGG + chr8_2:12178383-12178402 None:intergenic 55.0%
! GCCTGATTGGCTGTGACTTC+AGG - chr8_2:12178741-12178760 Msa1234640:intron 55.0%
TCCCAGTGCATTCCTTAGCC+TGG + chr8_2:12180033-12180052 None:intergenic 55.0%
GGCTAAGGAATGCACTGGGA+AGG - chr8_2:12180033-12180052 Msa1234640:intron 55.0%
ACCAAACACCCTAGCGTGCA+AGG - chr8_2:12180086-12180105 Msa1234640:intron 55.0%
! ACCTTGCACGCTAGGGTGTT+TGG + chr8_2:12180090-12180109 None:intergenic 55.0%
ACCCTAGCGTGCAAGGTACA+TGG - chr8_2:12180093-12180112 Msa1234640:intron 55.0%
GTCCATGTACCTTGCACGCT+AGG + chr8_2:12180098-12180117 None:intergenic 55.0%
TGCTGACGGTGATCGGAGAC+GGG + chr8_2:12177644-12177663 None:intergenic 60.0%
CTGCTGACGGTGATCGGAGA+CGG + chr8_2:12177645-12177664 None:intergenic 60.0%
GGCGGTTGTTGGACTGCTGA+CGG + chr8_2:12177658-12177677 None:intergenic 60.0%
GGGTTCGCCTCCTTAAACGC+TGG + chr8_2:12179799-12179818 None:intergenic 60.0%
GGAGGCGAACCCTCAGTTAG+AGG - chr8_2:12179807-12179826 Msa1234640:intron 60.0%
GGCGAACCCTCAGTTAGAGG+TGG - chr8_2:12179810-12179829 Msa1234640:intron 60.0%
TGAGCTGTGCGGCGGCGTTA+GGG + chr8_2:12177614-12177633 None:intergenic 65.0%
CTGACGGTGATCGGAGACGG+GGG + chr8_2:12177642-12177661 None:intergenic 65.0%
GCTGACGGTGATCGGAGACG+GGG + chr8_2:12177643-12177662 None:intergenic 65.0%
GTGAGCTGTGCGGCGGCGTT+AGG + chr8_2:12177615-12177634 None:intergenic 70.0%
GGGCTCTGTGAGCTGTGCGG+CGG + chr8_2:12177622-12177641 None:intergenic 70.0%
CGGGGGCTCTGTGAGCTGTG+CGG + chr8_2:12177625-12177644 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 12177471 12180530 12177471 ID=Msa1234640;Name=Msa1234640
chr8_2 mRNA 12177471 12180530 12177471 ID=Msa1234640-mRNA-1;Parent=Msa1234640;Name=Msa1234640-mRNA-1;_AED=0.02;_eAED=0.02;_QI=216|1|1|1|1|1|3|394|119
chr8_2 exon 12177471 12178037 12177471 ID=Msa1234640-mRNA-1:exon:4726;Parent=Msa1234640-mRNA-1
chr8_2 exon 12178121 12178227 12178121 ID=Msa1234640-mRNA-1:exon:4725;Parent=Msa1234640-mRNA-1
chr8_2 exon 12180235 12180530 12180235 ID=Msa1234640-mRNA-1:exon:4724;Parent=Msa1234640-mRNA-1
chr8_2 five_prime_UTR 12180315 12180530 12180315 ID=Msa1234640-mRNA-1:five_prime_utr;Parent=Msa1234640-mRNA-1
chr8_2 CDS 12180235 12180314 12180235 ID=Msa1234640-mRNA-1:cds;Parent=Msa1234640-mRNA-1
chr8_2 CDS 12178121 12178227 12178121 ID=Msa1234640-mRNA-1:cds;Parent=Msa1234640-mRNA-1
chr8_2 CDS 12177865 12178037 12177865 ID=Msa1234640-mRNA-1:cds;Parent=Msa1234640-mRNA-1
chr8_2 three_prime_UTR 12177471 12177864 12177471 ID=Msa1234640-mRNA-1:three_prime_utr;Parent=Msa1234640-mRNA-1
Gene Sequence

>Msa1234640

ATGGCATTGCGAGGAGTTTGGCAACTGAAGAAGCTCGTTGTTAGCTATAGCGATTGGGGTGGAAGTAGCAAAGGCATTAGGGCATTTATGGAGTCGCATTTGCCAGCGTTTAAGGAGGCGAACCCTCAGTTAGAGGTGGACACTGAAATGATTCGTGGTCAGCATCCACATCTAAAGGCTTTTTACAAGAACCATAATGACCGAGTGGTGTGTGTGAAGAATATGGATCCGGAAGAGATACTTCTGCATGCAACCAGGCTAAGGAATGCACTGGGAAGGAAAGTAATTAAGCTGAGGACAAGACATGTAACCAAACACCCTAGCGTGCAAGGTACATGGACAACTGCTTTGAAATATTAG
Protein sequence

>Msa1234640

MALRGVWQLKKLVVSYSDWGGSSKGIRAFMESHLPAFKEANPQLEVDTEMIRGQHPHLKAFYKNHNDRVVCVKNMDPEEILLHATRLRNALGRKVIKLRTRHVTKHPSVQGTWTTALKY*