AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1238990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1238990 MtrunA17_Chr8g0350741 96.629 178 5 1 1 177 1 178 7.07e-127 353
Msa1238990 MtrunA17_Chr7g0246761 70.455 176 46 3 1 171 1 175 1.97e-85 249
Msa1238990 MtrunA17_Chr1g0198201 41.497 147 84 2 1 146 1 146 2.89e-37 128
Msa1238990 MtrunA17_Chr3g0118071 33.884 121 70 3 17 131 30 146 1.31e-18 79.7
Msa1238990 MtrunA17_Chr4g0013561 53.125 64 28 2 71 134 94 155 3.80e-18 78.6
Msa1238990 MtrunA17_Chr3g0118061 34.454 119 68 3 22 134 35 149 8.98e-18 77.4
Msa1238990 MtrunA17_Chr6g0486651 31.429 175 90 8 8 170 43 199 4.75e-17 77.4
Msa1238990 MtrunA17_Chr1g0184631 39.326 89 48 1 88 176 96 178 5.44e-17 74.3
Msa1238990 MtrunA17_Chr7g0274711 32.934 167 96 4 15 176 22 177 8.04e-17 73.9
Msa1238990 MtrunA17_Chr1g0197021 33.582 134 77 4 4 131 14 141 8.31e-17 73.6
Msa1238990 MtrunA17_Chr6g0451161 44.737 76 41 1 59 134 130 204 1.01e-16 76.6
Msa1238990 MtrunA17_Chr8g0381731 39.362 94 48 2 84 177 169 253 3.23e-16 74.3
Msa1238990 MtrunA17_Chr1g0167811 29.293 198 105 10 3 176 27 213 5.19e-16 73.9
Msa1238990 MtrunA17_Chr8g0364881 32.090 134 83 3 8 135 9 140 6.65e-16 73.9
Msa1238990 MtrunA17_Chr8g0383931 34.579 107 62 2 72 170 124 230 1.68e-15 71.6
Msa1238990 MtrunA17_Chr5g0413841 40.260 77 45 1 59 135 113 188 2.38e-15 72.4
Msa1238990 MtrunA17_Chr3g0128771 32.520 123 75 3 20 134 42 164 2.55e-15 70.1
Msa1238990 MtrunA17_Chr5g0411081 30.657 137 84 4 8 134 58 193 2.60e-15 72.4
Msa1238990 MtrunA17_Chr2g0325141 28.358 134 84 3 8 131 2 133 2.60e-15 69.3
Msa1238990 MtrunA17_Chr1g0197031 31.579 133 84 3 4 131 14 144 2.83e-15 69.7
Msa1238990 MtrunA17_Chr4g0067591 36.765 68 40 1 64 131 82 146 3.55e-15 71.2
Msa1238990 MtrunA17_Chr7g0248831 44.928 69 37 1 64 131 82 150 4.15e-15 69.3
Msa1238990 MtrunA17_Chr8g0383901 37.209 86 51 1 72 154 114 199 4.85e-15 70.1
Msa1238990 MtrunA17_Chr5g0427431 33.000 100 63 2 64 163 136 231 5.76e-15 71.6
Msa1238990 MtrunA17_Chr8g0383881 36.471 85 54 0 56 140 97 181 6.92e-15 69.7
Msa1238990 MtrunA17_Chr8g0366561 30.178 169 106 7 8 169 58 221 7.07e-15 71.2
Msa1238990 MtrunA17_Chr2g0327791 38.750 80 48 1 59 137 104 183 7.71e-15 69.7
Msa1238990 MtrunA17_Chr1g0184171 36.620 71 45 0 64 134 105 175 9.33e-15 70.9
Msa1238990 MtrunA17_Chr5g0409211 60.465 43 17 0 89 131 94 136 1.03e-14 68.6
Msa1238990 MtrunA17_Chr2g0325161 38.356 73 40 1 64 131 93 165 1.15e-14 69.3
Msa1238990 MtrunA17_Chr4g0030831 43.478 69 38 1 64 131 86 154 1.25e-14 68.2
Msa1238990 MtrunA17_Chr1g0186661 37.838 74 41 1 66 134 103 176 1.32e-14 70.5
Msa1238990 MtrunA17_Chr7g0276591 41.096 73 37 2 64 134 78 146 1.43e-14 69.7
Msa1238990 MtrunA17_Chr7g0248841 41.667 72 39 1 66 134 78 149 1.49e-14 67.8
Msa1238990 MtrunA17_Chr3g0144241 44.615 65 34 1 70 134 87 149 2.23e-14 67.8
Msa1238990 MtrunA17_Chr8g0345361 35.185 108 54 3 64 171 94 185 2.31e-14 68.9
Msa1238990 MtrunA17_Chr3g0105441 53.191 47 22 0 88 134 131 177 2.32e-14 68.9
Msa1238990 MtrunA17_Chr5g0426781 52.083 48 23 0 84 131 149 196 2.61e-14 69.7
Msa1238990 MtrunA17_Chr7g0273051 38.356 73 43 1 64 134 93 165 2.78e-14 69.7
Msa1238990 MtrunA17_Chr8g0383861 36.471 85 54 0 56 140 107 191 3.37e-14 68.2
Msa1238990 MtrunA17_Chr6g0470751 40.845 71 41 1 88 157 125 195 4.11e-14 68.9
Msa1238990 MtrunA17_Chr7g0261091 33.784 74 46 1 64 134 72 145 4.14e-14 68.9
Msa1238990 MtrunA17_Chr1g0203541 37.349 83 42 2 64 139 107 186 4.90e-14 67.4
Msa1238990 MtrunA17_Chr5g0407431 32.609 92 52 2 53 134 59 150 6.32e-14 67.0
Msa1238990 MtrunA17_Chr8g0383871 34.884 86 53 1 68 150 118 203 8.14e-14 67.0
Msa1238990 MtrunA17_Chr8g0383921 34.884 86 53 1 68 150 117 202 9.27e-14 66.6
Msa1238990 MtrunA17_Chr7g0274741 52.273 44 21 0 88 131 98 141 9.27e-14 65.9
Msa1238990 MtrunA17_Chr1g0191601 43.243 74 41 1 88 160 126 199 9.69e-14 68.2
Msa1238990 MtrunA17_Chr2g0279431 49.057 53 27 0 84 136 156 208 1.02e-13 68.2
Msa1238990 MtrunA17_Chr4g0059281 35.526 76 48 1 59 134 103 177 1.09e-13 67.4
Msa1238990 MtrunA17_Chr8g0369571 41.026 78 36 2 86 163 87 154 1.19e-13 65.5
Msa1238990 MtrunA17_Chr3g0132491 53.191 47 22 0 88 134 135 181 1.56e-13 67.4
Msa1238990 MtrunA17_Chr3g0113811 33.582 134 74 5 7 134 27 151 1.98e-13 65.1
Msa1238990 MtrunA17_Chr8g0383941 35.514 107 62 2 68 167 113 219 2.07e-13 65.9
Msa1238990 MtrunA17_Chr5g0430781 40.000 80 42 2 52 131 79 152 2.73e-13 65.5
Msa1238990 MtrunA17_Chr7g0275601 62.791 43 16 0 89 131 108 150 2.79e-13 64.3
Msa1238990 MtrunA17_Chr5g0396601 62.791 43 16 0 89 131 350 392 3.20e-13 66.6
Msa1238990 MtrunA17_Chr5g0426881 53.191 47 22 0 88 134 142 188 3.48e-13 66.6
Msa1238990 MtrunA17_Chr3g0126931 41.096 73 40 2 88 159 126 196 3.54e-13 66.2
Msa1238990 MtrunA17_Chr2g0295571 32.927 82 44 2 68 139 99 179 4.31e-13 65.9
Msa1238990 MtrunA17_Chr1g0177041 37.879 66 39 1 68 131 80 145 4.59e-13 63.9
Msa1238990 MtrunA17_Chr5g0409201 55.814 43 19 0 89 131 93 135 4.78e-13 63.5
Msa1238990 MtrunA17_Chr4g0050041 34.568 81 50 2 53 131 115 194 5.05e-13 65.9
Msa1238990 MtrunA17_Chr4g0073141 30.000 100 69 1 59 157 114 213 5.62e-13 66.2
Msa1238990 MtrunA17_Chr3g0120011 41.176 68 38 1 67 134 99 164 5.63e-13 64.3
Msa1238990 MtrunA17_Chr5g0426871 52.174 46 22 0 89 134 137 182 6.72e-13 65.9
Msa1238990 MtrunA17_Chr1g0197011 56.410 39 17 0 93 131 111 149 7.07e-13 63.2
Msa1238990 MtrunA17_Chr8g0336881 33.333 72 46 1 65 134 79 150 9.35e-13 65.1
Msa1238990 MtrunA17_Chr8g0388231 32.031 128 72 2 13 131 258 379 1.13e-12 65.1
Msa1238990 MtrunA17_Chr7g0244851 45.455 55 30 0 80 134 98 152 1.22e-12 62.8
Msa1238990 MtrunA17_Chr8g0348151 55.556 45 20 0 87 131 112 156 1.59e-12 63.2
Msa1238990 MtrunA17_Chr8g0388201 58.140 43 18 0 89 131 347 389 1.64e-12 64.7
Msa1238990 MtrunA17_Chr3g0133001 36.364 66 41 1 66 131 134 198 1.68e-12 64.7
Msa1238990 MtrunA17_Chr3g0118101 47.917 48 25 0 88 135 113 160 1.95e-12 62.4
Msa1238990 MtrunA17_Chr3g0143301 29.268 123 64 4 15 134 14 116 1.98e-12 61.2
Msa1238990 MtrunA17_Chr5g0396611 60.465 43 17 0 89 131 340 382 2.26e-12 64.3
Msa1238990 MtrunA17_Chr1g0196991 53.333 45 21 0 93 137 120 164 2.72e-12 61.6
Msa1238990 MtrunA17_Chr7g0274721 39.062 64 37 1 68 131 74 135 3.59e-12 61.6
Msa1238990 MtrunA17_Chr8g0370171 46.667 45 24 0 87 131 96 140 3.82e-12 62.0
Msa1238990 MtrunA17_Chr8g0347461 37.681 69 41 1 68 134 117 185 3.91e-12 63.2
Msa1238990 MtrunA17_Chr5g0444141 53.488 43 20 0 89 131 220 262 4.16e-12 62.8
Msa1238990 MtrunA17_Chr8g0347101 62.791 43 16 0 89 131 314 356 4.35e-12 63.2
Msa1238990 MtrunA17_Chr4g0027761 39.130 69 38 1 66 134 104 168 4.54e-12 61.6
Msa1238990 MtrunA17_Chr5g0396591 58.140 43 18 0 89 131 240 282 4.91e-12 62.8
Msa1238990 MtrunA17_Chr3g0129671 44.444 54 30 0 87 140 134 187 5.26e-12 62.0
Msa1238990 MtrunA17_Chr3g0081611 52.273 44 21 0 88 131 107 150 6.47e-12 60.8
Msa1238990 MtrunA17_Chr5g0444161 48.837 43 22 0 89 131 185 227 7.09e-12 61.6
Msa1238990 MtrunA17_Chr7g0274731 48.837 43 22 0 89 131 114 156 8.31e-12 60.8
Msa1238990 MtrunA17_Chr5g0437361 39.344 61 37 0 74 134 88 148 1.09e-11 60.1
Msa1238990 MtrunA17_Chr3g0118091 50.000 44 22 0 88 131 66 109 1.47e-11 58.9
Msa1238990 MtrunA17_Chr2g0301871 45.455 44 24 0 88 131 82 125 1.58e-11 61.2
Msa1238990 MtrunA17_Chr1g0157091 48.936 47 24 0 88 134 70 116 1.70e-11 58.9
Msa1238990 MtrunA17_Chr8g0366521 29.231 130 80 4 11 134 28 151 2.65e-11 60.8
Msa1238990 MtrunA17_Chr3g0123641 35.366 82 51 1 88 167 74 155 2.73e-11 60.1
Msa1238990 MtrunA17_Chr5g0406031 25.000 124 78 3 21 131 110 231 3.87e-11 59.7
Msa1238990 MtrunA17_Chr4g0060981 51.111 45 22 0 87 131 172 216 4.29e-11 59.3
Msa1238990 MtrunA17_Chr3g0123631 35.526 76 47 1 88 161 74 149 5.97e-11 58.5
Msa1238990 MtrunA17_Chr2g0297011 39.062 64 38 1 68 131 82 144 7.36e-11 57.8
Msa1238990 MtrunA17_Chr5g0433861 29.771 131 78 4 11 134 28 151 7.47e-11 59.7
Msa1238990 MtrunA17_Chr2g0317671 43.478 46 26 0 86 131 156 201 7.72e-11 58.5
Msa1238990 MtrunA17_Chr3g0117821 40.000 50 30 0 87 136 83 132 8.85e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1238990 AT5G40250.1 33.333 129 63 3 58 169 101 223 4.97e-17 77.8
Msa1238990 AT4G10160.1 52.632 57 26 1 78 134 87 142 7.03e-17 75.5
Msa1238990 AT3G16720.1 42.647 68 37 1 64 131 96 161 1.19e-16 76.3
Msa1238990 AT4G10150.1 56.250 48 21 0 87 134 109 156 1.52e-16 74.7
Msa1238990 AT5G57750.1 28.689 122 87 0 55 176 88 209 1.70e-16 74.3
Msa1238990 AT3G48030.2 30.814 172 102 6 3 160 35 203 2.87e-16 74.7
Msa1238990 AT3G48030.1 30.128 156 93 5 19 160 128 281 3.82e-16 75.1
Msa1238990 AT4G15975.1 31.193 109 64 2 65 173 54 151 9.93e-16 72.8
Msa1238990 AT1G20823.1 29.268 123 82 1 17 134 34 156 1.11e-15 71.6
Msa1238990 AT1G72220.1 28.378 148 99 4 8 149 93 239 1.11e-15 73.9
Msa1238990 AT4G09110.1 51.667 60 29 0 88 147 122 181 1.64e-15 72.8
Msa1238990 AT1G33480.1 46.875 64 32 2 71 134 84 145 1.83e-15 72.4
Msa1238990 AT3G14320.1 30.303 132 66 3 64 171 65 194 2.63e-15 70.9
Msa1238990 AT2G35910.1 65.116 43 15 0 89 131 147 189 4.52e-15 70.5
Msa1238990 AT5G01880.1 50.980 51 25 0 84 134 100 150 5.11e-15 69.3
Msa1238990 AT4G17905.1 49.180 61 30 1 74 134 141 200 6.37e-15 71.2
Msa1238990 AT1G22500.1 44.156 77 36 2 65 140 99 169 6.91e-15 71.6
Msa1238990 AT3G03550.1 38.636 88 50 2 84 170 154 238 8.42e-15 71.2
Msa1238990 AT5G05280.1 43.750 64 34 1 71 134 97 158 8.49e-15 68.9
Msa1238990 AT4G17905.2 49.180 61 30 1 74 134 167 226 9.19e-15 71.2
Msa1238990 AT1G35330.1 57.447 47 20 0 88 134 128 174 1.01e-14 70.9
Msa1238990 AT2G46160.1 41.333 75 37 1 64 131 106 180 1.39e-14 69.3
Msa1238990 AT2G46160.2 41.333 75 37 1 64 131 106 180 1.39e-14 69.3
Msa1238990 AT3G10910.1 46.154 52 28 0 83 134 108 159 1.84e-14 68.2
Msa1238990 AT2G34990.1 32.308 130 81 4 8 134 16 141 2.21e-14 69.7
Msa1238990 AT2G34990.2 32.308 130 81 4 8 134 38 163 2.22e-14 70.1
Msa1238990 AT2G42360.1 34.234 111 66 2 21 131 47 150 2.45e-14 68.9
Msa1238990 AT4G09100.1 54.545 44 20 0 88 131 84 127 2.62e-14 66.6
Msa1238990 AT2G27940.1 35.443 79 49 1 53 131 106 182 3.34e-14 68.6
Msa1238990 AT1G72200.1 42.500 80 39 2 65 143 125 198 3.75e-14 69.7
Msa1238990 AT4G33565.1 40.299 67 39 1 65 131 194 259 4.04e-14 69.3
Msa1238990 AT1G23980.1 29.730 148 87 5 6 137 46 192 4.32e-14 69.3
Msa1238990 AT2G42350.1 40.506 79 43 2 53 131 68 142 4.79e-14 67.8
Msa1238990 AT2G20030.1 35.354 99 60 3 68 165 104 199 4.91e-14 69.3
Msa1238990 AT4G09120.1 57.447 47 20 0 88 134 122 168 6.23e-14 68.9
Msa1238990 AT1G76410.1 41.538 65 38 0 70 134 85 149 6.83e-14 66.6
Msa1238990 AT5G17600.1 34.524 84 54 1 54 137 108 190 6.86e-14 68.9
Msa1238990 AT1G04360.1 39.726 73 38 1 68 134 107 179 1.10e-13 68.2
Msa1238990 AT5G43420.1 34.831 89 45 2 56 131 92 180 1.32e-13 68.2
Msa1238990 AT2G17450.1 49.020 51 26 0 87 137 100 150 1.40e-13 65.9
Msa1238990 AT3G05200.1 49.153 59 29 1 88 145 127 185 1.74e-13 67.8
Msa1238990 AT5G10380.1 46.429 56 30 0 87 142 133 188 1.94e-13 67.0
Msa1238990 AT5G58580.1 35.366 82 52 1 64 144 113 194 2.12e-13 67.0
Msa1238990 AT2G35420.1 35.200 125 68 6 56 171 73 193 2.29e-13 66.6
Msa1238990 AT5G05810.1 31.429 105 58 3 66 163 124 221 2.62e-13 67.4
Msa1238990 AT4G30400.1 35.616 73 47 0 59 131 104 176 2.85e-13 67.4
Msa1238990 AT2G18650.1 34.211 76 50 0 59 134 96 171 3.20e-13 67.0
Msa1238990 AT5G47610.1 29.412 119 76 3 18 134 40 152 3.79e-13 64.3
Msa1238990 AT4G28890.1 40.625 64 37 1 68 131 103 165 3.84e-13 67.0
Msa1238990 AT4G09130.1 57.447 47 20 0 88 134 119 165 3.93e-13 66.6
Msa1238990 AT4G30370.1 32.184 87 58 1 59 145 86 171 5.00e-13 64.3
Msa1238990 AT4G35480.1 50.980 51 25 0 87 137 111 161 5.62e-13 64.7
Msa1238990 AT3G18773.1 32.727 110 71 1 31 140 75 181 7.18e-13 64.7
Msa1238990 AT3G61550.1 31.481 108 64 1 34 131 71 178 7.20e-13 64.7
Msa1238990 AT1G72310.1 40.299 67 38 1 68 134 108 172 8.22e-13 65.5
Msa1238990 AT4G40070.1 38.356 73 43 1 64 134 97 169 9.11e-13 65.5
Msa1238990 AT2G18670.1 38.462 65 40 0 67 131 88 152 9.64e-13 63.5
Msa1238990 AT1G74410.1 48.077 52 27 0 85 136 172 223 1.03e-12 64.3
Msa1238990 AT3G20395.1 45.902 61 30 1 71 131 156 213 1.09e-12 64.3
Msa1238990 AT2G47560.1 53.488 43 20 0 89 131 108 150 1.11e-12 64.3
Msa1238990 AT5G06490.1 53.488 43 20 0 89 131 129 171 1.22e-12 63.5
Msa1238990 AT1G53820.1 41.818 55 32 0 88 142 119 173 1.25e-12 65.1
Msa1238990 AT3G62690.1 50.000 44 22 0 88 131 112 155 1.92e-12 63.9
Msa1238990 AT5G53110.1 62.791 43 16 0 89 131 330 372 2.46e-12 64.3
Msa1238990 AT3G18930.2 45.283 53 29 0 82 134 149 201 2.92e-12 64.3
Msa1238990 AT3G18930.3 45.283 53 29 0 82 134 149 201 2.92e-12 64.3
Msa1238990 AT3G18930.1 45.283 53 29 0 82 134 149 201 2.92e-12 64.3
Msa1238990 AT5G27420.1 46.429 56 30 0 88 143 123 178 3.19e-12 63.9
Msa1238990 AT3G19140.1 36.765 68 41 1 64 131 56 121 3.22e-12 61.2
Msa1238990 AT2G35000.1 48.936 47 24 0 88 134 134 180 3.66e-12 63.9
Msa1238990 AT2G46493.1 55.814 43 19 0 89 131 138 180 3.88e-12 62.0
Msa1238990 AT1G49220.1 47.170 53 28 0 88 140 134 186 9.76e-12 62.0
Msa1238990 AT1G49230.1 46.296 54 29 0 87 140 129 182 1.04e-11 61.6
Msa1238990 AT1G28040.1 58.140 43 18 0 89 131 253 295 1.84e-11 61.6
Msa1238990 AT1G49210.1 47.170 53 28 0 88 140 134 186 1.89e-11 60.8
Msa1238990 AT3G60220.1 32.955 88 54 2 52 134 75 162 2.11e-11 61.6
Msa1238990 AT5G66070.5 25.424 118 81 2 21 131 101 218 2.23e-11 60.5
Msa1238990 AT5G66070.1 25.424 118 81 2 21 131 101 218 2.23e-11 60.5
Msa1238990 AT4G26580.1 55.102 49 21 1 88 136 288 335 2.67e-11 61.2
Msa1238990 AT4G26580.2 55.102 49 21 1 88 136 288 335 2.67e-11 61.2
Msa1238990 AT2G25410.1 58.140 43 18 0 89 131 327 369 2.91e-11 61.2
Msa1238990 AT5G07040.1 53.488 43 20 0 89 131 94 136 3.02e-11 59.3
Msa1238990 AT1G49200.1 45.283 53 29 0 88 140 135 187 3.53e-11 60.1
Msa1238990 AT3G11110.1 33.803 71 47 0 64 134 82 152 3.69e-11 58.9
Msa1238990 AT5G66070.2 44.681 47 26 0 85 131 196 242 3.94e-11 60.1
Msa1238990 AT2G46494.1 55.814 43 19 0 89 131 316 358 4.01e-11 60.8
Msa1238990 AT2G46495.2 55.814 43 19 0 89 131 292 334 4.57e-11 60.8
Msa1238990 AT1G53010.1 33.945 109 66 2 32 134 67 175 4.86e-11 58.9
Msa1238990 AT2G46495.1 55.814 43 19 0 89 131 320 362 5.94e-11 60.5
Msa1238990 AT4G24015.1 53.488 43 20 0 89 131 105 147 7.85e-11 58.5
Msa1238990 AT2G28920.1 28.972 107 73 1 28 131 29 135 9.55e-11 57.4

Find 30 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTGGTTACTACAATTAT+TGG 0.178264 8_2:+20001804 Msa1238990:CDS
GTTACTACAATTATTGGATT+TGG 0.246283 8_2:+20001810 Msa1238990:CDS
GATGAGTTCAAGTAACAATT+TGG 0.262569 8_2:+20001788 Msa1238990:CDS
GGGTCATTACCATGATATTC+TGG 0.303976 8_2:-20002349 None:intergenic
AAATTTACCCGTTCAATATC+TGG 0.310230 8_2:-20001936 None:intergenic
TACAGGTGTGTAATATGTTT+AGG 0.346782 8_2:+20003332 Msa1238990:intron
TGAAAAGTGTGTCCACAATA+AGG 0.364822 8_2:-20003389 None:intergenic
GCAGCAACAAATGCAGGTTC+AGG 0.381451 8_2:-20002370 None:intergenic
ATCTTTCTTGCATTGATATG+TGG 0.409101 8_2:+20003411 Msa1238990:CDS
ATCACGTACTATATATGAAA+TGG 0.409592 8_2:+20001905 Msa1238990:CDS
CTCATCAAGAGATTGTCTTA+TGG 0.419558 8_2:-20003519 None:intergenic
TTTGTGGAAGATTTAGAGAA+AGG 0.422289 8_2:+20001874 Msa1238990:CDS
GAAGTTGATGATATAGAAGT+AGG 0.476212 8_2:+20003606 Msa1238990:exon
GGGATTGCAGCAACAAATGC+AGG 0.491563 8_2:-20002376 None:intergenic
TACTTTACATTTCAATCAAG+AGG 0.498877 8_2:+20002399 Msa1238990:CDS
TCTTGATTGAAATGTAAAGT+AGG 0.503985 8_2:-20002397 None:intergenic
ATCAAGACCAGATATTGAAC+GGG 0.505726 8_2:+20001929 Msa1238990:CDS
TGTAATGTCAAACGACATAC+AGG 0.509118 8_2:-20003455 None:intergenic
TGTCTTATGGTGAATGTCAC+AGG 0.511603 8_2:-20003506 None:intergenic
CTTGATTGAAATGTAAAGTA+GGG 0.516939 8_2:-20002396 None:intergenic
CAGCAACAAATGCAGGTTCA+GGG 0.527717 8_2:-20002369 None:intergenic
GTATGTACAAGATTAATTTG+TGG 0.546575 8_2:+20001858 Msa1238990:CDS
ACATGATAGCCAGAATATCA+TGG 0.564569 8_2:+20002340 Msa1238990:intron
GATGAGTCTAATACTTCAGA+AGG 0.576330 8_2:+20003536 Msa1238990:CDS
AATCAAGACCAGATATTGAA+CGG 0.579390 8_2:+20001928 Msa1238990:CDS
TTGCGCATAATACCTTATTG+TGG 0.599434 8_2:+20003377 Msa1238990:CDS
AGATTTAGAGAAAGGCATGT+GGG 0.648166 8_2:+20001882 Msa1238990:CDS
CAAACGACATACAGGACATG+TGG 0.652651 8_2:-20003447 None:intergenic
AAGATTTAGAGAAAGGCATG+TGG 0.684425 8_2:+20001881 Msa1238990:CDS
TGTACACTGAAGATGACTGA+AGG 0.704247 8_2:-20002422 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTAAAAAAAAAAAA+TGG - chr8_2:20002510-20002529 None:intergenic 0.0%
!!! TAATTTTTAAAAAAAAAAAA+TGG - chr8_2:20002510-20002529 None:intergenic 0.0%
!! AAGAAATATTATATATTAAG+AGG - chr8_2:20002192-20002211 None:intergenic 10.0%
!! GTAAAAAAAAAAAAAATGTT+TGG + chr8_2:20002597-20002616 Msa1238990:intron 10.0%
!!! TTTTCTAAAAAAAATCTAAT+AGG + chr8_2:20003221-20003240 Msa1238990:intron 10.0%
!! ATAAAAAAAATAGCTAAATT+TGG - chr8_2:20003262-20003281 None:intergenic 10.0%
!! AAGAAATATTATATATTAAG+AGG - chr8_2:20002192-20002211 None:intergenic 10.0%
!! GTAAAAAAAAAAAAAATGTT+TGG + chr8_2:20002597-20002616 Msa1238990:intron 10.0%
!!! TTTTCTAAAAAAAATCTAAT+AGG + chr8_2:20003221-20003240 Msa1238990:intron 10.0%
!! ATAAAAAAAATAGCTAAATT+TGG - chr8_2:20003262-20003281 None:intergenic 10.0%
!!! CAAATTTTTTTTGATTTTTC+TGG + chr8_2:20002079-20002098 Msa1238990:intron 15.0%
!!! AATAAAATCTTATTTGAAAC+AGG + chr8_2:20002538-20002557 Msa1238990:intron 15.0%
!!! ATAGATTAGTTAAAAAAGTA+TGG - chr8_2:20002890-20002909 None:intergenic 15.0%
!!! ATTTAGAAAAGAACAAATTT+TGG - chr8_2:20003298-20003317 None:intergenic 15.0%
!!! TTCTTTTCTAAATTTCATTT+TGG + chr8_2:20003305-20003324 Msa1238990:intron 15.0%
!!! CAAATTTTTTTTGATTTTTC+TGG + chr8_2:20002079-20002098 Msa1238990:intron 15.0%
!!! AATAAAATCTTATTTGAAAC+AGG + chr8_2:20002538-20002557 Msa1238990:intron 15.0%
!!! ATAGATTAGTTAAAAAAGTA+TGG - chr8_2:20002890-20002909 None:intergenic 15.0%
!!! ATTTAGAAAAGAACAAATTT+TGG - chr8_2:20003298-20003317 None:intergenic 15.0%
!!! TTCTTTTCTAAATTTCATTT+TGG + chr8_2:20003305-20003324 Msa1238990:intron 15.0%
!!! GTTTTTTGTTCTAAAAAGAT+TGG + chr8_2:20001733-20001752 Msa1238990:five_prime_UTR 20.0%
!! AATTTGGTTACTACAATTAT+TGG + chr8_2:20001804-20001823 Msa1238990:CDS 20.0%
!!! AAAAAAAATGGCTTTTATGA+GGG - chr8_2:20002498-20002517 None:intergenic 20.0%
!!! AAAAAAAAATGGCTTTTATG+AGG - chr8_2:20002499-20002518 None:intergenic 20.0%
!!! GTAATTTGTTTGTAATTTTG+TGG - chr8_2:20002790-20002809 None:intergenic 20.0%
!! TAATCTAATAAGACAAAGTT+AGG + chr8_2:20003151-20003170 Msa1238990:intron 20.0%
!!! AATTTCATTTTGGTTTTTAC+AGG + chr8_2:20003315-20003334 Msa1238990:intron 20.0%
!!! GTTTTTTGTTCTAAAAAGAT+TGG + chr8_2:20001733-20001752 Msa1238990:five_prime_UTR 20.0%
!! AATTTGGTTACTACAATTAT+TGG + chr8_2:20001804-20001823 Msa1238990:CDS 20.0%
!!! AAAAAAAATGGCTTTTATGA+GGG - chr8_2:20002498-20002517 None:intergenic 20.0%
!!! AAAAAAAAATGGCTTTTATG+AGG - chr8_2:20002499-20002518 None:intergenic 20.0%
!!! GTAATTTGTTTGTAATTTTG+TGG - chr8_2:20002790-20002809 None:intergenic 20.0%
!! TAATCTAATAAGACAAAGTT+AGG + chr8_2:20003151-20003170 Msa1238990:intron 20.0%
!!! AATTTCATTTTGGTTTTTAC+AGG + chr8_2:20003315-20003334 Msa1238990:intron 20.0%
! GTTACTACAATTATTGGATT+TGG + chr8_2:20001810-20001829 Msa1238990:CDS 25.0%
!! GTATGTACAAGATTAATTTG+TGG + chr8_2:20001858-20001877 Msa1238990:CDS 25.0%
! ATCACGTACTATATATGAAA+TGG + chr8_2:20001905-20001924 Msa1238990:CDS 25.0%
!!! TTTTGTTGTTGTTTATGTCA+TGG + chr8_2:20002268-20002287 Msa1238990:intron 25.0%
! ATGGTGTAAAATGTTTAAGT+TGG + chr8_2:20002287-20002306 Msa1238990:intron 25.0%
!!! TGTAAAATGTTTAAGTTGGA+TGG + chr8_2:20002291-20002310 Msa1238990:intron 25.0%
! CTTGATTGAAATGTAAAGTA+GGG - chr8_2:20002399-20002418 None:intergenic 25.0%
! TCTTGATTGAAATGTAAAGT+AGG - chr8_2:20002400-20002419 None:intergenic 25.0%
! TACTTTACATTTCAATCAAG+AGG + chr8_2:20002399-20002418 Msa1238990:CDS 25.0%
!!! TTTGGAGTAAACAATCATTT+TGG + chr8_2:20002615-20002634 Msa1238990:intron 25.0%
!!! ATTGGACTTGTATTTTGAAA+CGG - chr8_2:20002831-20002850 None:intergenic 25.0%
! TGTTGAATTTAGATAGGATT+TGG - chr8_2:20003120-20003139 None:intergenic 25.0%
! GTTACTACAATTATTGGATT+TGG + chr8_2:20001810-20001829 Msa1238990:CDS 25.0%
!! GTATGTACAAGATTAATTTG+TGG + chr8_2:20001858-20001877 Msa1238990:CDS 25.0%
! ATCACGTACTATATATGAAA+TGG + chr8_2:20001905-20001924 Msa1238990:CDS 25.0%
!!! TTTTGTTGTTGTTTATGTCA+TGG + chr8_2:20002268-20002287 Msa1238990:intron 25.0%
! ATGGTGTAAAATGTTTAAGT+TGG + chr8_2:20002287-20002306 Msa1238990:intron 25.0%
!!! TGTAAAATGTTTAAGTTGGA+TGG + chr8_2:20002291-20002310 Msa1238990:intron 25.0%
! CTTGATTGAAATGTAAAGTA+GGG - chr8_2:20002399-20002418 None:intergenic 25.0%
! TCTTGATTGAAATGTAAAGT+AGG - chr8_2:20002400-20002419 None:intergenic 25.0%
! TACTTTACATTTCAATCAAG+AGG + chr8_2:20002399-20002418 Msa1238990:CDS 25.0%
!!! TTTGGAGTAAACAATCATTT+TGG + chr8_2:20002615-20002634 Msa1238990:intron 25.0%
!!! ATTGGACTTGTATTTTGAAA+CGG - chr8_2:20002831-20002850 None:intergenic 25.0%
! TGTTGAATTTAGATAGGATT+TGG - chr8_2:20003120-20003139 None:intergenic 25.0%
!!! AAAAGATTGGTTTTGTGAAG+TGG + chr8_2:20001746-20001765 Msa1238990:five_prime_UTR 30.0%
!!! AAAGATTGGTTTTGTGAAGT+GGG + chr8_2:20001747-20001766 Msa1238990:five_prime_UTR 30.0%
GATGAGTTCAAGTAACAATT+TGG + chr8_2:20001788-20001807 Msa1238990:CDS 30.0%
TTTGTGGAAGATTTAGAGAA+AGG + chr8_2:20001874-20001893 Msa1238990:CDS 30.0%
AATCAAGACCAGATATTGAA+CGG + chr8_2:20001928-20001947 Msa1238990:CDS 30.0%
AAATTTACCCGTTCAATATC+TGG - chr8_2:20001939-20001958 None:intergenic 30.0%
TTTGAACTTAGCAATGTTTG+AGG + chr8_2:20002118-20002137 Msa1238990:intron 30.0%
TTAGCAATGTTTGAGGAAAA+AGG + chr8_2:20002125-20002144 Msa1238990:intron 30.0%
TAGCAATGTTTGAGGAAAAA+GGG + chr8_2:20002126-20002145 Msa1238990:intron 30.0%
GTGATGCTTGAGTATTAATT+AGG + chr8_2:20002229-20002248 Msa1238990:intron 30.0%
!!! GTAAACAATCATTTTGGTTC+TGG + chr8_2:20002621-20002640 Msa1238990:intron 30.0%
CAAATGAAAGATACATTTCG+AGG - chr8_2:20002757-20002776 None:intergenic 30.0%
!! CTTGGACATGTATTTTGAAA+CGG + chr8_2:20003053-20003072 Msa1238990:intron 30.0%
TGAATTTAGATAGGATTTGG+AGG - chr8_2:20003117-20003136 None:intergenic 30.0%
TCCTATCTAAATTCAACACA+TGG + chr8_2:20003122-20003141 Msa1238990:intron 30.0%
TCCATGTGTTGAATTTAGAT+AGG - chr8_2:20003126-20003145 None:intergenic 30.0%
TACAGGTGTGTAATATGTTT+AGG + chr8_2:20003332-20003351 Msa1238990:intron 30.0%
! ATCTTTCTTGCATTGATATG+TGG + chr8_2:20003411-20003430 Msa1238990:CDS 30.0%
!! GAAGTTGATGATATAGAAGT+AGG + chr8_2:20003606-20003625 Msa1238990:exon 30.0%
!!! AAAAGATTGGTTTTGTGAAG+TGG + chr8_2:20001746-20001765 Msa1238990:five_prime_UTR 30.0%
!!! AAAGATTGGTTTTGTGAAGT+GGG + chr8_2:20001747-20001766 Msa1238990:five_prime_UTR 30.0%
GATGAGTTCAAGTAACAATT+TGG + chr8_2:20001788-20001807 Msa1238990:CDS 30.0%
TTTGTGGAAGATTTAGAGAA+AGG + chr8_2:20001874-20001893 Msa1238990:CDS 30.0%
AATCAAGACCAGATATTGAA+CGG + chr8_2:20001928-20001947 Msa1238990:CDS 30.0%
AAATTTACCCGTTCAATATC+TGG - chr8_2:20001939-20001958 None:intergenic 30.0%
TTTGAACTTAGCAATGTTTG+AGG + chr8_2:20002118-20002137 Msa1238990:intron 30.0%
TTAGCAATGTTTGAGGAAAA+AGG + chr8_2:20002125-20002144 Msa1238990:intron 30.0%
TAGCAATGTTTGAGGAAAAA+GGG + chr8_2:20002126-20002145 Msa1238990:intron 30.0%
GTGATGCTTGAGTATTAATT+AGG + chr8_2:20002229-20002248 Msa1238990:intron 30.0%
!!! GTAAACAATCATTTTGGTTC+TGG + chr8_2:20002621-20002640 Msa1238990:intron 30.0%
CAAATGAAAGATACATTTCG+AGG - chr8_2:20002757-20002776 None:intergenic 30.0%
!! CTTGGACATGTATTTTGAAA+CGG + chr8_2:20003053-20003072 Msa1238990:intron 30.0%
TGAATTTAGATAGGATTTGG+AGG - chr8_2:20003117-20003136 None:intergenic 30.0%
TCCTATCTAAATTCAACACA+TGG + chr8_2:20003122-20003141 Msa1238990:intron 30.0%
TCCATGTGTTGAATTTAGAT+AGG - chr8_2:20003126-20003145 None:intergenic 30.0%
TACAGGTGTGTAATATGTTT+AGG + chr8_2:20003332-20003351 Msa1238990:intron 30.0%
! ATCTTTCTTGCATTGATATG+TGG + chr8_2:20003411-20003430 Msa1238990:CDS 30.0%
!! GAAGTTGATGATATAGAAGT+AGG + chr8_2:20003606-20003625 Msa1238990:exon 30.0%
!!! AAGATTGGTTTTGTGAAGTG+GGG + chr8_2:20001748-20001767 Msa1238990:five_prime_UTR 35.0%
! AAGATTTAGAGAAAGGCATG+TGG + chr8_2:20001881-20001900 Msa1238990:CDS 35.0%
! AGATTTAGAGAAAGGCATGT+GGG + chr8_2:20001882-20001901 Msa1238990:CDS 35.0%
ATCAAGACCAGATATTGAAC+GGG + chr8_2:20001929-20001948 Msa1238990:CDS 35.0%
ACATGATAGCCAGAATATCA+TGG + chr8_2:20002340-20002359 Msa1238990:intron 35.0%
!! TTCTAGAACCAAACACACTA+AGG - chr8_2:20002709-20002728 None:intergenic 35.0%
!!! GACTTGTATTTTGAAACGGA+GGG - chr8_2:20002827-20002846 None:intergenic 35.0%
ATAGTGCACACTGCAAAAAT+TGG - chr8_2:20002849-20002868 None:intergenic 35.0%
TAACGGTCAAAATTGTGCAT+TGG + chr8_2:20003012-20003031 Msa1238990:intron 35.0%
CATATGTGAAGTGATTGACT+TGG + chr8_2:20003035-20003054 Msa1238990:intron 35.0%
!!! GACATGTATTTTGAAACGGA+GGG + chr8_2:20003057-20003076 Msa1238990:intron 35.0%
TTGCGCATAATACCTTATTG+TGG + chr8_2:20003377-20003396 Msa1238990:CDS 35.0%
! TGAAAAGTGTGTCCACAATA+AGG - chr8_2:20003392-20003411 None:intergenic 35.0%
TGTAATGTCAAACGACATAC+AGG - chr8_2:20003458-20003477 None:intergenic 35.0%
CTCATCAAGAGATTGTCTTA+TGG - chr8_2:20003522-20003541 None:intergenic 35.0%
GATGAGTCTAATACTTCAGA+AGG + chr8_2:20003536-20003555 Msa1238990:CDS 35.0%
!!! AAGATTGGTTTTGTGAAGTG+GGG + chr8_2:20001748-20001767 Msa1238990:five_prime_UTR 35.0%
! AAGATTTAGAGAAAGGCATG+TGG + chr8_2:20001881-20001900 Msa1238990:CDS 35.0%
! AGATTTAGAGAAAGGCATGT+GGG + chr8_2:20001882-20001901 Msa1238990:CDS 35.0%
ATCAAGACCAGATATTGAAC+GGG + chr8_2:20001929-20001948 Msa1238990:CDS 35.0%
ACATGATAGCCAGAATATCA+TGG + chr8_2:20002340-20002359 Msa1238990:intron 35.0%
!! TTCTAGAACCAAACACACTA+AGG - chr8_2:20002709-20002728 None:intergenic 35.0%
!!! GACTTGTATTTTGAAACGGA+GGG - chr8_2:20002827-20002846 None:intergenic 35.0%
ATAGTGCACACTGCAAAAAT+TGG - chr8_2:20002849-20002868 None:intergenic 35.0%
TAACGGTCAAAATTGTGCAT+TGG + chr8_2:20003012-20003031 Msa1238990:intron 35.0%
CATATGTGAAGTGATTGACT+TGG + chr8_2:20003035-20003054 Msa1238990:intron 35.0%
!!! GACATGTATTTTGAAACGGA+GGG + chr8_2:20003057-20003076 Msa1238990:intron 35.0%
TTGCGCATAATACCTTATTG+TGG + chr8_2:20003377-20003396 Msa1238990:CDS 35.0%
! TGAAAAGTGTGTCCACAATA+AGG - chr8_2:20003392-20003411 None:intergenic 35.0%
TGTAATGTCAAACGACATAC+AGG - chr8_2:20003458-20003477 None:intergenic 35.0%
CTCATCAAGAGATTGTCTTA+TGG - chr8_2:20003522-20003541 None:intergenic 35.0%
GATGAGTCTAATACTTCAGA+AGG + chr8_2:20003536-20003555 Msa1238990:CDS 35.0%
!!! AGATTGGTTTTGTGAAGTGG+GGG + chr8_2:20001749-20001768 Msa1238990:five_prime_UTR 40.0%
GGGTCATTACCATGATATTC+TGG - chr8_2:20002352-20002371 None:intergenic 40.0%
TGTACACTGAAGATGACTGA+AGG - chr8_2:20002425-20002444 None:intergenic 40.0%
GAGTCTTTCCTTAGTGTGTT+TGG + chr8_2:20002698-20002717 Msa1238990:intron 40.0%
!!! GGACTTGTATTTTGAAACGG+AGG - chr8_2:20002828-20002847 None:intergenic 40.0%
!!! GGACATGTATTTTGAAACGG+AGG + chr8_2:20003056-20003075 Msa1238990:intron 40.0%
TGTCTTATGGTGAATGTCAC+AGG - chr8_2:20003509-20003528 None:intergenic 40.0%
!!! AGATTGGTTTTGTGAAGTGG+GGG + chr8_2:20001749-20001768 Msa1238990:five_prime_UTR 40.0%
GGGTCATTACCATGATATTC+TGG - chr8_2:20002352-20002371 None:intergenic 40.0%
TGTACACTGAAGATGACTGA+AGG - chr8_2:20002425-20002444 None:intergenic 40.0%
GAGTCTTTCCTTAGTGTGTT+TGG + chr8_2:20002698-20002717 Msa1238990:intron 40.0%
!!! GGACTTGTATTTTGAAACGG+AGG - chr8_2:20002828-20002847 None:intergenic 40.0%
!!! GGACATGTATTTTGAAACGG+AGG + chr8_2:20003056-20003075 Msa1238990:intron 40.0%
TGTCTTATGGTGAATGTCAC+AGG - chr8_2:20003509-20003528 None:intergenic 40.0%
!!! GATTGGTTTTGTGAAGTGGG+GGG + chr8_2:20001750-20001769 Msa1238990:five_prime_UTR 45.0%
CAGCAACAAATGCAGGTTCA+GGG - chr8_2:20002372-20002391 None:intergenic 45.0%
CAAACGACATACAGGACATG+TGG - chr8_2:20003450-20003469 None:intergenic 45.0%
!!! GATTGGTTTTGTGAAGTGGG+GGG + chr8_2:20001750-20001769 Msa1238990:five_prime_UTR 45.0%
CAGCAACAAATGCAGGTTCA+GGG - chr8_2:20002372-20002391 None:intergenic 45.0%
CAAACGACATACAGGACATG+TGG - chr8_2:20003450-20003469 None:intergenic 45.0%
!! TATATAATTAAAGATATTAA+CGG + chr8_2:20002995-20003014 Msa1238990:intron 5.0%
!! TATATAATTAAAGATATTAA+CGG + chr8_2:20002995-20003014 Msa1238990:intron 5.0%
!! TTTTGTGAAGTGGGGGGTGT+GGG + chr8_2:20001756-20001775 Msa1238990:five_prime_UTR 50.0%
GCAGCAACAAATGCAGGTTC+AGG - chr8_2:20002373-20002392 None:intergenic 50.0%
GGGATTGCAGCAACAAATGC+AGG - chr8_2:20002379-20002398 None:intergenic 50.0%
!! TTTTGTGAAGTGGGGGGTGT+GGG + chr8_2:20001756-20001775 Msa1238990:five_prime_UTR 50.0%
GCAGCAACAAATGCAGGTTC+AGG - chr8_2:20002373-20002392 None:intergenic 50.0%
GGGATTGCAGCAACAAATGC+AGG - chr8_2:20002379-20002398 None:intergenic 50.0%
! GTTTTGTGAAGTGGGGGGTG+TGG + chr8_2:20001755-20001774 Msa1238990:five_prime_UTR 55.0%
! GTTTTGTGAAGTGGGGGGTG+TGG + chr8_2:20001755-20001774 Msa1238990:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 20001719 20003629 20001719 ID=Msa1238990;Name=Msa1238990
chr8_2 mRNA 20001719 20003629 20001719 ID=Msa1238990-mRNA-1;Parent=Msa1238990;Name=Msa1238990-mRNA-1;_AED=0.06;_eAED=0.06;_QI=67|1|1|1|0|0|3|22|177
chr8_2 exon 20001719 20001950 20001719 ID=Msa1238990-mRNA-1:exon:7329;Parent=Msa1238990-mRNA-1
chr8_2 exon 20002349 20002446 20002349 ID=Msa1238990-mRNA-1:exon:7330;Parent=Msa1238990-mRNA-1
chr8_2 exon 20003337 20003629 20003337 ID=Msa1238990-mRNA-1:exon:7331;Parent=Msa1238990-mRNA-1
chr8_2 five_prime_UTR 20001719 20001785 20001719 ID=Msa1238990-mRNA-1:five_prime_utr;Parent=Msa1238990-mRNA-1
chr8_2 CDS 20001786 20001950 20001786 ID=Msa1238990-mRNA-1:cds;Parent=Msa1238990-mRNA-1
chr8_2 CDS 20002349 20002446 20002349 ID=Msa1238990-mRNA-1:cds;Parent=Msa1238990-mRNA-1
chr8_2 CDS 20003337 20003607 20003337 ID=Msa1238990-mRNA-1:cds;Parent=Msa1238990-mRNA-1
chr8_2 three_prime_UTR 20003608 20003629 20003608 ID=Msa1238990-mRNA-1:three_prime_utr;Parent=Msa1238990-mRNA-1
Gene Sequence

>Msa1238990

ATGATGAGTTCAAGTAACAATTTGGTTACTACAATTATTGGATTTGGATTGAGTGCAACTTTCATTGTATTTGTATGTACAAGATTAATTTGTGGAAGATTTAGAGAAAGGCATGTGGGATCACGTACTATATATGAAATGGAATCAAGACCAGATATTGAACGGCCAGAATATCATGGTAATGACCCTGAACCTGCATTTGTTGCTGCAATCCCTACTTTACATTTCAATCAAGAGGCCTTCAGTCATCTTCAGTGTACACAGTGTGTAATATGTTTAGGAGAGTACAAAGAAAAAGAAATATTGCGCATAATACCTTATTGTGGACACACTTTTCATCTTTCTTGCATTGATATGTGGCTAAGAAAACAATCCACATGTCCTGTATGTCGTTTGACATTACATAATGTTTGTGAAGAAAAACATGTGAGACCTGTGACATTCACCATAAGACAATCTCTTGATGAGTCTAATACTTCAGAAGGTAATGAGAGACAAGTTGAAGTTAATGCAAGAGAAACAGAATCAAGCTGA
Protein sequence

>Msa1238990

MMSSSNNLVTTIIGFGLSATFIVFVCTRLICGRFRERHVGSRTIYEMESRPDIERPEYHGNDPEPAFVAAIPTLHFNQEAFSHLQCTQCVICLGEYKEKEILRIIPYCGHTFHLSCIDMWLRKQSTCPVCRLTLHNVCEEKHVRPVTFTIRQSLDESNTSEGNERQVEVNARETESS*